磷酸化激酶抗体芯片

震惊！畅销款磷酸化激酶抗体芯片原来长这样
蛋白激酶(Protein kinases)是细胞功能的关键调节分子，构成了最大且功能最多样的基因家族之一。

通过添加磷酸酶组到底物蛋白，可指导许多蛋白的活动、定位以及总体功能，并且参与编排几乎所有的细胞活动。

因此，激酶活性的异常是包括癌症在内的许多疾病的基础。

R&D Systems 的磷酸化激酶抗体芯片一直位居芯片销量排行榜榜首，小编日常接到的查询是这样的：
ARY003B多少钱？
ARY003B可以做几个样？
ARY003B 可筛查的因子清单发一个给我…
此芯片包含了人的常见信号通路的43个重要节点，是筛选信号通路的有利工具。

打开R&D Systems 官网，该货号的评分和文献引用数量是这样的：
五星好评！
忍不住再深入了解下
Proteome Profiler Human Phospho-Kinase Array Kit——人磷酸化激酶抗体芯片，包含了8张膜（每组两张膜），可以检测4个样本：
适用的样本类型（具体用量需参考产品详细说明书）：
每张膜复孔点喷45种抗激酶相关抗体：
分析数据结果示例：
Figure 1. MCF-7 human breast cancer cells were either left untreated or exposed to 50 J/m2 of UV light followed by a 4 hour recovery before lysis.
Figure 2. A431 human epithelial carcinoma cells were either left untreated or treated with 200 ng/mL recombinant human (rh) EGF (Catalog # 236-EG) for 5 minutes.
Figure 3. MCF-7 human breast cancer cells were either left untreated or treated with 100 ng/mL
of rhIGF-I (Catalog # 291-G1) for 1 hour.
试剂盒内包含的组分有：
∙8 Array Membranes (4 of membrane A and 4 of membrane B)
∙8-Well Multi-dish
∙Lysis Buffer
∙Array Buffers
∙Wash Buffer
∙Detection Antibody Cocktail A
∙Detection Antibody Cocktail B
∙Streptavidin-HRP
∙Chemiluminescent Detection Reagents
∙Transparency Overlay Template
∙Detailed product datasheet with protocol
操作步骤：1. 加样孵育过夜；2.加检测抗体混合液，孵育2h；3. 加HRP标记的链霉亲和素和化学发光试剂，孵育30min；4. 曝光读取数据。

实验时间仅需5.5个小时。

使用此产品发表的最新文献：
1. Dysregulation of Mir-196b in Head and Neck Cancers Leads to Pleiotropic Effects in the
Tumor Cells and Surrounding Stromal Fibroblasts
Authors: S Álvarez-Te, ST Menéndez, MÁ Villaronga, JP Rodrigo, L Manterola, L de Villala, JC de Vicente, L Alonso-Dur, MP Fernández, CH Lawrie, JM García-Ped
Sci Rep, 2017;7(1):17785. 2017
2. Uric acid priming in human monocytes is driven by the AKT-PRAS40 autophagy pathway
Authors: TO Cri?an, MCP Cleophas, B Novakovic, K Erler, FL van de Vee, HG Stunnenber, MG Netea, CA Dinarello, LAB Joosten
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017
3. EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells
through STAT3 and HIF2�
Authors: II Kuan, KH Liang, YP Wang, TW Kuo, YJ Meir, SC Wu, SC Yang, J Lu, HC Wu Sci Rep, 2017;7(0):41852. 2017
4. Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated
by ADAM10 and ADAM17
Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz J. Biol. Chem, 2017;0(0):. 2017
5. Structural Determinants of the Gain-of-Function Phenotype of Human Leukemia-associated
Mutant CBL Oncogene
Authors: SA Nadeau, W An, BC Mohapatra, I Mushtaq, TA Bielecki, H Luan, N Zutshi, G Ahmad, MD Storck, M Sanada, S Ogawa, V Band, H Band
J. Biol. Chem, 2017;292(9):3666-3682. 2017
6. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors
and Rheumatoid Arthritis Patients.
Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
J Immunol, 2017;198(4):1512-1520. 2017
7. Antagonizing effects of membrane-acting androgens on the eicosanoid receptor OXER1 in
prostate cancer
Authors: K Kalyvianak, V Gebhart, N Peroulis, C Panagiotop, F Kiagiadaki, I Pediaditak, M Aivaliotis, E Moustou, M Tzardi, G Notas, E Castanas, M Kampa
Sci Rep, 2017;7(0):44418. 2017
8. Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration,
and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer.
Authors: Chang C, Yen M, Liao S, Hsu Y, Lai C, Chang K, Hsu Y
Int J Mol Sci, 2017;18(7):. 2017
9. Galectin-3 interacts with the cell surface glycoprotein CD146 (MCAM, MUC18) and induces
secretion of metastasis-promoting cytokines from vascular endothelial cells
Authors: F Colomb, W Wang, D Simpson, M Zafar, R Beynon, JM Rhodes, LG Yu
J. Biol. Chem., 2017;0(0):. 2017
10. The HIV-1 viral synapse signals human foreskin keratinocytes to secrete thymic stromal
lymphopoietin facilitating HIV-1 foreskin entry
Authors: Z Zhou, L Xu, A Sennepin, C Federici, Y Ganor, D Tudor, D Damotte, N Barry Delo, M Zerbib, M Bomsel
Mucosal Immunol, 2017;0(0):. 2017
11. Activation of p38 and Erk Mitogen-Activated Protein Kinases Signaling in Ocular Rosacea
Authors: EJ Wladis, S Swamy, A Herrmann, J Yang, JA Carlson, AP Adam
Invest. Ophthalmol. Vis. Sci, 2017;58(2):843-848. 2017
更多文献请咨询上海优宁维生物科技股份有限公司。