ol-37-14-2970 Light sources generating far fields
赛默飞近红外 参数
赛默飞近红外参数【实用版】目录一、赛默飞近红外光谱仪的概述二、赛默飞近红外光谱仪的参数1.Nicolet iS 5N2.Trudefender 手持红外光谱仪三、赛默飞近红外光谱仪的应用领域四、赛默飞近红外光谱仪的优势五、结语正文一、赛默飞近红外光谱仪的概述赛默飞近红外光谱仪是一款高性能的光谱分析仪器,能够对样品进行近红外区域的光谱分析,被广泛应用于各个领域,如化学、生物学、医学、环境监测等。
赛默飞作为全球知名的科学仪器制造商,在近红外光谱仪领域有着丰富的经验和技术积累。
二、赛默飞近红外光谱仪的参数1.Nicolet iS 5Nicolet iS 5N 是赛默飞一款先进的近红外光谱仪,具有高分辨率、高灵敏度和快速扫描等特点。
其主要参数如下:- 波长范围:0.9μm ~ 1.7μm- 分辨率:≤0.5cm^-1- 灵敏度:≤0.001g/mL- 扫描速度:≤1s/点2.Trudefender 手持红外光谱仪Trudefender 是赛默飞一款手持式近红外光谱仪,具有便携、易操作和实时分析等特点。
其主要参数如下:- 波长范围:0.9μm ~ 1.7μm- 分辨率:≤1.5cm^-1- 灵敏度:≤0.01g/mL- 扫描速度:≤2s/点- 重量:≤3kg三、赛默飞近红外光谱仪的应用领域赛默飞近红外光谱仪广泛应用于各种领域,如生物医学、化学化工、食品饮料、环境和材料科学等。
在生物医学领域,可以用于蛋白质结构分析、疾病诊断和生物分子识别等;在化学化工领域,可以用于分子结构分析、化学反应监测和产品质量控制等;在食品饮料领域,可以用于成分分析、品质控制和食品安全监测等。
四、赛默飞近红外光谱仪的优势赛默飞近红外光谱仪具有以下优势:1.高分辨率和高灵敏度,能够对样品进行精确分析。
2.快速扫描和实时分析,能够提高分析效率。
3.便携式设计,便于携带和现场分析。
4.丰富的应用经验和技术支持,确保分析结果的准确性。
五、结语赛默飞近红外光谱仪凭借其优异的性能和广泛的应用领域,在光谱分析领域具有重要地位。
OCT(3种)
SPECIAL NOTE:OCT Microscope NowBeta Testing atSelected Customers.Commercial Availability-January 2006.■Optical Coherence Microscope . . . . . . . . . . . . . . . . . 2■Rapidly Swept T unable Laser■Spectral Radar Optical Coherence TImaging Specifications2D Cross-sectional OCT Imaging Capability Imaging Speed: 25 Frames Per Second (Based on 500 Axial Scans Per Frame)Maximum Image Size: 800 (W) x 512 (H) pixels Maximum Imaging Width: 6mm OCM1300SS Microscope System: Capable of simultaneous cross-sectional OCT imaging and conventional en-face microscope imaging as well as three-dimensional imaging of the sample.Sales: 973-579-7227The OCT system has sensitivity of 108 dB. The coherence length of the laser is measured to be >7 mm, which supports OCT imagingdepth of > 3 mm. The FWHW of the point-spread function of the interference fringes is measured to be ~12 µm for both forward and backward scan, suggest effective axial resolution of ~ 9 µm in tissue.Forward scan point spread function from seven A-scans each with a different delay.Backward scan point spread function from seven A-scans each with a different delay.Sales: 973-579-7227An OCT signal processing board is developed to accelerate the calibration of fringe signals from time to frequency. The clock boardprocesses the MZI clock signal to generate pulses equally spaced in frequency. The digitizer is configured in external clock mode and uses the clock pulses as time base to sample OCT fringe signals with data points linear in frequency.Backward scan point spread function showing the resolution of the system in air.Forward scan point spread function showing the resolution of the system in air.Real time video-rate imagingReal-time video-rate imaging speed with 17-30 frames/second based on 500 axial scans per frame and 1024 points Fast Fourier T ransform(FFT) can be achieved in the swept source OCT system.Results2D tomographic images of in-vivo human skin(d) palm(e) back of handOCT interferencefringes point spread function diagnosis (1D mode).3D volume imaging mode (500 x 500 x 500pixels) with en-face imaging capability.2D cross-sectional imaging mode (500 x 500 pixels)OCT ChannelCCD ChannelSales: 973-579-7227A 3 mm x 3 mm x 3mm volume containing 500 x 500 x 500 pixels3D imaging of the sample (top to bottom: IR card, screw, leaf, skin )Potential Applications1Biology and medical imaging2.3D optical profilometry3.Material inspection and product quality control4.Thin film test and measurement5.Other non-invasive laser imaging applicationsReferencesR. Huber, M. Wojtkowski, J. G. Fujimoto, J. Y. Jiang, and A. E. Cable, “Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm”, submitted to Opt.Exp. 2005.AcknowledgementsWe acknowledge scientific discussions and helpful advice from Dr. Robert Huber and Prof. James G.Fujimoto at Research Laboratory of Electronic of Massachusetts Institute of Technology.Sales: 973-579-7227Swept Source Optical Coherence T omography (SS-OCT) applications require a laser that can be swept over a wide wavelength interval with very high speed. The wide wavelength range is required for obtaining high image resolution, and the high tuning frequency is needed for obtaining video image rates.Thorlabs is pleased to offer a fast sweeping, continuous wavelength, external cavity laser source specifically designed for SS-OCT applications. The standard system sweeps at least 100nm at a 16kHz repetition rate, offers a coherence length of 6mm and delivers more than 12mW of average optical power out of an SMF28 single mode fiber.The laser is available with the following fixed scanning frequency, coherence length and tuning range:■SL1325-P16-16KHz scanning frequency, 6mm coherence length, 120nm tuning rangeThe SL1325 comes with a Mach-Zender interferometer (MZI) with an adjustable free spectral range of 50 to 100 GHz. The MZI is used to digitally resample the raw Optical Coherence T omography (OCT) signal into equally spaced points in frequency. BNC connectors are available to monitor the wavelength sweep direction and laser intensity signals. The SL1325 comes in a 19 inch rack unit configured for 115VAC or 220/240 VAC.OCT is a relatively recent and fundamentally new way of obtaining high-resolution images in turbid media. Various OCT techniques are employed in dermatology, surgery (surgical guidance), and in ophthalmology. One of the driving forces of thedevelopment of the various OCT techniques is to find methods for in-vivo histology. However, the OCT techniques can be used for other types of characterization and visualizing of structures in turbid media, for example, materials research. The development of OCT started in the 1990’s and is a continuously growing field of research and usage.OCT image of in-vivo finger tissue OCT image of in-vivo palm tissueSL1325-P16Sales: 973-579-7227PARAMETERMIN TYPICAL MAX COMMENTSWavelength Range (nm)1265–1385Center Wavelength (nm)131513251340T uning Range (nm) (-10dB cut off point)100–140See models aboveRepetition Rate15–17See models aboveT uning Speed Continuous (nm/ms)–2000–Mean tuning speed around centerwavelengthAverage Optical Output Power (mW)101215Averaged over 1 secondOptical Power Stability (dB)––±0.5Forward to forward scan or backward backward scan during 1 hour usageSignal Source Spontaneous Emission Ratio (SSE) (dB)–25–Optical Isolation (dB)50––Operating Temperature + 10°C –+ 40°C Storage Temperature0°C –+ 70°C Diff. Optical Power at Forward and Back Scan(%)–20–Coherence Length (mm)6–8See models aboveLinear Polarization –>80 : 1–Measured at laser output facet Repetition Rate (kHz)101620Other rates on request Return Loss (dB)–45–Physical Size600 x 450 x 300Width x Depth x HeightSpectrum of the OCT swept laser showing an active wavelength tuning range of 155nm centered around 1325nm.MZI Clock: The frequency clock of the swept laser isfrom a build-in Mach-Zehnder interferometer with balanced detector output. The zero-crossings, as well as maxima and minima, of the interference fringe signals,are equally spaced in frequency and can be used asfrequency clock to synchronize other measurements.For Details on Our Full Lineof OCT ProductsVisit us at An OCT cross-section of a human fingernailSales: 973-579-7227HandheldScanning ProbeImaging Specifications (Other Options Available):■Imaging Speed:. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Frames per Second Image Depth:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.6 mm Photograph A - Spectral Radar OCT Imaging SystemWith our system in mind, for a fixed position of the reference mirror, and a fixedFigure I -Schematic Device100nm FWHMFFTLD 930nmGratingS pectrometerbalances the desire for low scattering losses within biological samples with the need for operating inside the wavelength range of commercial silicon based linear sensor arrays. Many biological samples provide sufficient transparency at this with our standard system is the handheld application system depicted in Photograph B.Figure V – Solid model, cross sectional view of the handheld scanner probe that contains the free-space interferometer and telecentric imaging optics.Figure IV – Photograph of the handheld scanning probe in use imaging a volunteers arm.Fig. VIV – SR-OCT Surface Measurement of a Sinusoidal Survace.In vitro images from porcine retina and nerve head.SR-OCT Image of a thumb.。
Spatiotemporal control of gene expression by a light-switchable transgene system
©2012 N a t u r e A m e r i c a , I n c . A l l r i g h t s r e s e r v e d.nature methods | ADVANCE ONLINE PUBLICATION | and a dimerization domain. Removal of the dimerization domain to create Gal4(65), which contains Gal4 residues 1–65, virtually eliminates binding to its consensus cognate DNA sequence, the upstream activating sequence of Gal (UASG )12. Vivid (VVD), the smallest light-oxygen-voltage (LOV) domain–containing protein, forms a rapidly exchanging dimer upon blue-light activation 13–15. We reasoned that the DNA-binding property of a Gal4(65)-VVD fusion protein would be light-switchable, as light should induce dimerization of the fusion protein, enhance binding to the UASG sequence and activate transcription and removing the light should result in gradual dissociation of the dimers, DNA dissociation and inactivation (Fig. 1a ). Indeed, an electrophoretic mobility shift assay showed that Gal4(65)-VVD dimerized upon 15 W m −2 constant blue-light illumination and bound the UASG sequence (Fig. 1b ).Purified Gal4(65)-VVD had similar spectra to VVD 13 in dark orlight states (Supplementary Fig. 1). These data suggested that the VVD domain in the fusion protein was correctly folded and was bound by flavin adenine dinucleotide (FAD) and that light illumination induced Gal4 dimerization and binding to UASG .To create a system capable of driving light-activated transcrip-tion, we fused different transactivation domains to the C terminus of Gal4(65)-VVD (Fig. 1a and Supplementary Fig. 2). We tested their light-dependent impact on transcriptional activity of a firefly luciferase (Fluc ) reporter driven by Gal4 binding sites upstream of a TATA box after transient transfection in HEK293 cells, illumina-tion with 0.84 W m −2 460 nm peak light from an LED lamp for 22 h and measurement of expression. Transactivators containing the p65 activation domain (GAVP) or the VP16 activation domain (GAVV) both showed marked light-induced reporter gene transcription, but the GAVP transactivator resulted in much greater gene expression under light exposure conditions (Fig. 1c and Supplementary Fig. 3). Mutation of Cys108 in VVD to serine blocked light-inducible gene expression as expected 13 (Fig. 1c and Supplementary Fig. 3). Mutation of Cys71 to valine in VVD is known to enhance the sta-bility of the light-induced VVD dimer 14, and based on the crystal structure of VVD 13 we hypothesized that mutating Gln56 of VVD to lysine would form a salt bridge with Asp68 of the other VVD protein and additionaly stabilize the dimer. Both dimer-enhancing mutations, C71V and N56K, in the VVD domain decreased reporter gene expression in the dark, whereas the N56K,C71Vd ouble mutant (optimized GAVP (GAVPO)) additionally decreased the background gene expression to a minimal level (Fig. 1c and Supplementary Note ). We used GAVPO in all subsequent studies, and we referred to the gene promoter system based on GAVPO as the light-on (LightOn) system.We compared the LightOn system to human cytomegalovirus immediate early promoter (CMV )-based induction of reporterspatiotemporal control of gene expression by a light-switchable transgene systemXue Wang, Xianjun Chen & Yi YangWe developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. the transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. this transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.Regulated transgene systems are indispensable tools in biomedical research and biotechnology. During the past decade, chemically regulated gene expression systems 1,2 have been widely used for the temporal control of gene expression. However, as these small molecular inducers diffuse freely and are hard to remove, it is not possible to precisely switch on and off gene expression at an exact location and time. In contrast to chemicals, light is an ideal inducer of gene expression because it is easy to obtain, highly tunable, non-toxic and, most importantly, has high spatiotemporal resolution. A light-switchable gene expression system could be the most prom-ising tool for precisely controlling spatiotemporal gene expression in multicellular organisms. There have been several efforts to con-trol gene expression using light. Caged transactivator or chemi-cal inducers that are activated by UV light have been developed, allowing study of gene function in developing embryos 3–5. Infrared laser light was used to induce heat shock–mediated expression of transgenes 6. Recently, synthetic approaches have been developed to regulate gene expression by light illumination using genetically encoded light sensors 7–11.Uptake of these methodologies by biolo-gists has been minimal, however, probably because of technical complexities or limitations. We sought to develop a simple robust transgene system that is directly regulated by a single genetically encoded, photosensitive transactivator.To create a light-switchable gene promoter system, it is necessary to first design a DNA-binding domain that is activated by light. The well-characterized DNA-binding domain comprising Gal4 residues 1–147, Gal4(147), consists of a DNA-recognition elementSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China. Correspondence should be addressed to Y.Y. (yiyang@).Received 19 July 2011; accepted 28 decembeR 2011; published online 12 febRuaRy 2012; doi:10.1038/nmeth.1892©2012 N a t u r e A m e r i c a , I n c . A l l r i g h t s r e s e r v e d . | ADVANCE ONLINE PUBLICATION | nature methodsbrief communicationsvectors driving Fluc , Gaussia princeps luciferase (Gluc), humanized recombinant GFP (hrGFP) or monomeric (m)Cherry protein expression by transient transfection of vectors encoding all components into HEK293 cells and light exposure as described above (Fig. 1d and Supplementary Figs. 4 and 5). We con-firmed that light-mediated activation required all components by western blots of Gluc (Fig. 1e ). We routinely observed 200–300-fold induction of Fluc and Gluc in our experiments and a similarly high on/off ratio of gene expression and induction efficiency of LightOn system in other cells lines (Fig. 1f ). The blue-light irradiation had little effect on expression of proteins whose tran-scription was driven by CMV promoter (Fig. 1d ), suggesting that the LightOn system has minimal interference with or toxicity to normal cellular function.We investigated the time course of light-induced Gluc tran-scription using quantitative real-time PCR. Cellular Gluc mRNA amounts increased 13-fold and 81-fold after 0.5 h and 1 h of light induction, with respect to samples not exposed to light (Fig. 2a ) followed by an increase in secreted Gluc protein ~1 h later (Fig. 2b ). Removal of light resulted in a slow decay in the amount of Gluc mRNA and a plateau in the amount of protein when we did not change the medium (Fig. 2a ,b ). Gluc expression depended on the duration of illumination, but a 30-min illumination was suf-ficient for ~25-fold induction of Gluc protein above background expression 10 h after initial illumination (Supplementary Fig. 6). Off expression kinetics of Gluc mRNA suggested that GAVPO was kept in the activated state with a half life of 2 h (Supplementary Fig. 7), which is similar to that of VVD (half life of ~18,000 s)16.GAVP VVD domain mutantsaW i l t y p e C 108N 56C 71N56K ,C7152383831171027652GAVPOGlucActinpGAVPO Light pU5-Gluc Size (kDa) e R e l a t i v e e x p r e s s i o n (%)f +++++––––––––––++++++MC F -7He pG 2MDA -MB -231P C -3dR e l a t i v e e x p r e s s i o n (%)figure | LightOn gene expression system. (a ) Schematic representation of the LightOn system. After light activation, GAVP homodimerizes, interactswith UASG elements (5xUASG) and initiates expression of the gene of interest. (b ) Electrophoretic mobility shift assay of binding between Gal4(65)-VVD (at indicated concentrations) and UASG DNA probe (125 nM)in the dark (left) or under 15 W m −2 constant blue light (right). (c ) Light- dependent activation of Fluc reporters based on GAVP with different mutations to enhance dimerization. (d ) Comparison of induction various reporters betweenthe LightOn system with GAVPO-driven genes and conventional vectors with CMV -driven genes. Fluc activity, hrGFP fluorescence and mCherry fluorescence in cell lysate were measured by chemiluminescence and fluorescence assay,respectively. Gluc activity in cell culture medium was measured by a chemiluminescence assay. The data in c and d were normalized to the expression levels of the same reporter protein expressed from vectors with CMV promotersin the dark. (e ) Western blot of the Gluc expression in HEK293 cells transiently transfected with pGAVPO and pU5-Gluc under light or dark conditions. (f ) Light-switchable Gluc expression from pGAVPO in different cell lines. The data were normalized to the expression of the same reporter protein expressed from the CMV promoter under light-on conditions. (c ,d ,f ) Error bars, mean ± s.e.m. (n = 4 samples) from the same experiment. Six (c ) or ten (d –f ) hours after transfection, cells were illuminated by 0.84 W m −2 blue light or remained in the dark for 22 h before measurements.Time (h)G l u c m R N A f o l d o f i n d u c t i o naTime (h)5.0 × 1071.0 × 1081.5 × 108bG l u c a c t i v i t y (R L U )Number of pulsesG l u c a c t i v i t y (R L U )4.0 × 108.0 × 101.2 × 101.6 × 10cG l u c a c t i v i t y (R L U )Pulse length (s)2 × 104 × 106 × 10dfigure | Time course of light-switchable gene expression using LightOn in HEK293 cells transiently transfected with pGAVPO and pU5-Gluc. (a ) Cellular Gluc mRNA level measured at indicated times in the dark, after illumination under continuous 0.84 W m −2 blue light or after illumination under 0.84 W m −2 blue light for 2 h and then in the dark (light-dark). (b ) Expression kinetics of the Gluc reporter in cell culture medium measured at indicated times in the dark, after illumination under continuous 0.84 W m −2 blue light or after illumination under blue light for 15 h and then in the dark (light-dark).Insets, kinetics of Gluc mRNA (a ) or protein activity (b ) during the 4 h or 3 h after the initial light exposure, respectively. (c ,d ) Gluc activity in medium, measured 4 h after the initial light exposure to blue-light pulses (c ; 10 s pulses, 22 W m −2, 8 min apart) or to a single blue-light pulse of varying duration (22 W m −2). RLU, relative luciferase units. Error bars, mean ± s.e.m. (n = 4 samples) from the same experiment.©2012 N a t u r e A m e r i c a , I n c . A l l r i g h t s r e s e r v e d .nature methods | ADVANCE ONLINE PUBLICATION | brief communicationsThis led to continued mRNA synthesis during the first few hours after turning the light off (Fig. 2a ). The estimated half life of Gluc mRNA was 10 h (Supplementary Fig. 7), which explained the continuous increase in the amount of Gluc in the medium after light was turned off for another 20 h (Fig. 2b ). To increase the off rate of the system we modified the 3′ untranslated region of the Gluc reporter gene by inserting the conserved AU-rich element (ARE) from the gene encoding GM-CSF, which medi-ates selective degradation of mRNA 17. Expression of Gluc-ARE stopped much earlier than did expression of the original Gluc gene (Supplementary Fig. 8). We also investigated the capacity to activate the LightOn system by short pulses of light and observed a strong dose dependence on the number of pulses and the dura-tion of a single pulse, showing that continuous illumination is unnecessary (Fig. 2c ,d ).We next tested the ability of the LightOn system to induce graded protein expression in cells by controlling the irradiance (Fig. 3 and Supplementary Figs. 9 and 10). To spatially control gene expression in cultured cells, we illuminated in a specific pat-tern HEK293 cells transfected with mCherry reporter and GA VPO . The mCherry fluorescence image of the cells had the pattern of the original image used as the mask (Supplementary Fig. 11).These data indicate that the LightOn system can be robustly used to quantitatively, spatially and temporally control gene expression in mammalian cells.Finally, we validated the LightOn system in vivo . We transferred GA VPO and mCherry reporter vector into the livers of mice using a hydrodynamic procedure. Exposure of the mice to blue light from below resulted in the appearance of marked fluorescence from mCherry protein in their livers (Fig. 4a ). Light-dependent transgene expression was limited to the anterior side and poste-rior lining of liver that received sufficient blue light irradiance, in contrast to controls transfected with pcDNA3.1 vector containing the mCherry gene driven by a CMV promoter that resulted in homogenous expression (Fig. 4a ). Light-induced expression of the mCherry gene in the liver was limited to 1 mm or less from the surface (Fig. 4b ). Spatial control of gene expression in the liver was possible with localized illumination using optical fibers (Supplementary Fig. 12).We then used the LightOn system for Cre recombinase–m ediated LacZ activation in Gt(ROSA)26Sor (ROSA26)-LacZ mice transfected with pGAVPO and pU5-Cre vectors. We observed LacZ expression in the liver after illumination with90 mW cm −2 blue light for 22 h and 48 h in the dark but not incontrol mice kept only in the dark (Fig. 4c ). This suggests that light-mediated, tissue-specific expression should be possible. As a very preliminary proof-of-principle demonstration of the poten-tial of our system for regulated gene or cell therapy, we transfected type I diabetic mice with pGAVPO and pU5-insulin vectors and observed that blue-light illumination caused a large drop of blood glucose compared to mice transfected with the vector encoding the nonfunctional GAVPO mutant (Fig. 4d ).An ideal regulated gene expression system should have low background expression, low toxicity, low interference with endogenous proteins or genes and the capacity for temporal and spatial control, and should be easy to manipulate. Most existing systems 3,5–11, however, do not simultaneously satisfy all of these above requirements (Supplementary Table 1). Approaches involv-ing caged activators 3,5 or heating effects 6 are hard to implementLight irradiance (W m –2)0.020.110.210.430.83figure | Graded response of mCherry expression under different blue-light irradiances. Ten hours after transfection of mCherry reporter andGAVPO vectors, cells were illuminated by blue light of indicated irradiances adjusted by neutral density filters for 22 h before determination.Fluorescence images are shown. Scale bar, 0.5 cm.aAnterior viewpcDNA3.1-mCherry; darkpU5-mCherry and pGAVPO; darkNo vector;darkpU5-mCherry and pGAVPO;blue lightFluorescenceFluorescencePosterior view bWhite lightcB l o o d g l u c o s e (m M )dAnterior viewPosterior viewDark LightWhite lightpcDNA3.1-mCherry;darkpU5-mCherry and pGAVPO;blue lightFluorescenceLightfigure 4 | Light-switchable transgene expression in mice. (a ,b ) Light induced mCherry transgene expression inwhole livers or kidneys (a ) or in cryosections (b ). Mice were transfected with no vector, pU5-mCherry andpGAVPO or pcDNA3.1-mCherry; then illuminated with 90 mW cm −2 blue light for 22 h or remained in the dark. Mice were then killed, and their livers and kidneys were dissected for mCherry fluorescence imaging. Scale bar, 1 cm (a ) and 0.5 mm (b ). (c ) Images of whole-mount X-gal staining of the lacZ expression in livers of ROSA26-LacZ reporter mice transfected with pGAVPO and pU5-Cre. Mice were illuminated with 90 mW cm −2 blue light for 22 h and then kept in the dark for another 48 h before measurements. Control mice receivedno light. Scale bar, 0.5 cm. (d ) Diabetic mice induced by streptozotocin were transfected with pU5-insulin together with pGAVPO or pGAVPO(C108S), which encodes the nonfunctional mutant. Mice were illuminatedwith 90 mW cm −2 blue light or kept in dark for 8 h. Glucose levels were measured after the mice rested in the dark for another 4 h with sufficient food. Error bars, s.e.m. (n = 8–10) from two independent experiments; statistics by two-tailed t test. *P < 0.04 versus ‘dark’ control; **P < 0.0002 versus ‘dark’ control.©2012 N a t u r e A m e r i c a , I n c . A l l r i g h t s r e s e r v e d .4 | ADVANCE ONLINE PUBLICATION | nature methodsbrief communicationsand manipulate, and are associated with potential problems of cell injury or side effects resulting from the UV-light irradiance or heat shock used to activate gene expression. The LightOn system reported here is based on a genetically encoded light sensor that uses FAD as a photon acceptor 13 and offers obvious advantages compared to the above techniques. As FAD naturally exists in cells, it is unnecessary to treat cells with extraneous ligands that are required by phytochrome 7 or caged activators 3,5. The single- chain 56-kDa genetically encoded light-switchable transactivator in the LightOn system, which operated through homodimeriza-tion, additionally reduced the complexity of the multicomponent gene expression methodologies based on two-hybrid technologies previously reported for yeast 7,10 or mammalian cells 9. Recently, a well-designed synthetic light-regulated circuit has been reported to regulate gene expression in transgenic cells and blood-glucose homeostasis in mice 11. However, as this technique is based on coupling an exogenously expressed blue light–induced melan-opsin receptor to major existing cellular signaling players such as phospholipase, phosphokinase and calcium, it suffers from low on/off ratio of gene expression and mutual interference with endogenous signaling events that may limit its usage. In contrast, the LightOn system is orthogonal to mammalian cellular signal-ing, which should allow tighter control with minimal perturba-tion of, or from, existing signaling pathways.There are many other advantages of the LightOn gene expres-sion system reported here. LightOn has low background and allows high induction with reasonably fast kinetics and revers-ibility. We showed that continuous illumination was not necessary to activate LightOn, and single brief pulses of light were sufficient. This was possible owing to the high induction level and low back-ground we observed. Because of the unusually stable photoacti-vated state of VVD 16, the LightOn system is extremely sensitive to light, thus minimizing any potential toxicity of blue-light irradi-ance on cells. We observed a fourfold increase in Fluc expression when we irradiated cells with blue light five orders of magnitude lower than the sun’s irradiance. These characteristics provide the capability for gene activation with good spatial, temporal and quantitative control in an easy-to-use and robust system. LightOn should be a powerful yet convenient tool for life science research, allowing spatial and temporal control of gene expression.In the past three years, optogenetics has become a booming field by using genetically encoded light-sensitive proteins to control the behavior of living cells and organisms 18,19. Most of these tools are based on light-gated ion channels, light-switchable enzymes or protein interactions. The LightOn system provides another general way to control biological processes using light-switchable gene expression, thus avoiding the need for case-by-case protein engineering to create light-regulated protein modules. In addi-tion to its use in mammals, the LightOn gene expression system could be used to control gene expression spatiotemporally in other model eukaryotes such as Danio rerio and Drosophilam elanogaster , in which Gal-UAS systems are already widely used to control cell type–specific gene expression. We anticipate thatthe LightOn system will be widely used in many fields of life sci-ence research and biotechnology that have great demand for high- resolution spatial and temporal control of gene expression.methodsMethods and any associated references are available in the online version of the paper at /naturemethods/.Note: Supplementary information is available on the Nature Methods website.acknoWledgmentsWe thank Z.H. Yu and J.Z. Chen for their suggestions, and Z.M. Du, Z.C. Ma, J.H. Wang, W.T. Zhu, X.Y. Feng and Y.Z. Zhao for technical assistance. This work was supported by the National Natural Science Foundation of China (grants 31170815, 31071260 and 90713026), the 863 Program (grant no. 2006AA02Z160), the Fok Ying Tung Education Foundation (grant 111022), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Dawn Program of Shanghai EducationCommission (grant 11SG31), Doctoral Fund of Ministry of Education of China (grant 20100074110010), the Fundamental Research Funds for the Central Universities and the 111 Project (grant B07023).author contributionsY.Y. conceived of the concept; Y.Y., X.W. and X.C. designed the experiments and analysed the data; X.W. preformed the molecular cloning, proteincharacterization and cell culture experiments; X.C. preformed animal studies; and Y.Y. wrote the manuscript.comPeting financial interestsThe authors declare no competing financial interests.Published online at /naturemethods/. reprints and permissions information is available online at /reprints/index.html.1. Braselmann, S., Graninger, P. & Busslinger, M. Proc. Natl. Acad. Sci. USA 90,1657–1661 (1993).2. Gossen, M. et al. Science 68, 1766–1769 (1995).3. Cambridge, S.B., Davis, R.L. & Minden, J.S. Science 77, 825–828(1997).4. Minden, J., Namba, R., Mergliano, J. & Cambridge, S. Sci. STKE 000, l1(2000).5. Cambridge, S.B. et al. Nat. Methods 6, 527–531 (2009).6. Kamei, Y. et al. Nat. Methods 6, 79–81 (2009).7. Shimizu-Sato, S., Huq, E., Tepperman, J.M. & Quail, P.H. Nat. Biotechnol. 0,1041–1044 (2002).8. Levskaya, A. et al. Nature 4 8, 441–442 (2005).9. Yazawa, M., Sadaghiani, A.M., Hsueh, B. & Dolmetsch, R.E. Nat. Biotechnol. 7,941–945 (2009).10. Kennedy, M.J. et al. Nat. Methods 7, 973–975 (2010).11. Ye, H., Daoud-El Baba, M., Peng, R.W. & Fussenegger, M. Science ,1565–1568 (2011).12. Hong, M. et al. Structure 6, 1019–1026 (2008).13. Zoltowski, B.D. et al. Science 6, 1054–1057 (2007).14. Zoltowski, B.D. & Crane, B.R. Biochemistry 47, 7012–7019 (2008).15. Lamb, J.S., Zoltowski, B.D., Pabit, S.A., Crane, B.R. & Pollack, L.J. Am. Chem. Soc. 0, 12226–12227 (2008).16. Zoltowski, B.D., Vaccaro, B. & Crane, B.R. Nat. Chem. Biol. 5, 827–834 (2009).17. Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).18. Deisseroth, K. Nat. Methods 8, 26–29 (2011).19. Fenno, L., Yizhar, O. & Deisseroth, K. Annu. Rev. Neurosci. 4, 389–412(2011).©2012 N a t u r e A m e r i c a , I n c . A l l r i g h t s r e s e r v e d .nature methodsdoi:10.1038/nmeth.1892online methodsDNA cloning. Full length VVD gene was isolated from Neurospora crassa (gift of B. Chen, Guangxi Normal University) genomic DNA with both introns and exons. Introns were removed by reverse PCR. Sequences encoding the Gal4(1–65), and VP16 acti-vation domain was amplified from pBIND and pACT (Promega), respectively. Chimeric fusion construct pGAVV consisting of sequences encoding Gal4(1–65), VVD (37–186) and VP16 acti-vation domain was generated using overlapping PCR and inserted into Eco47III and BsrGI sites in pEGFP-N1 vector. To generate chimeric fusion construct pGAVP , sequence encoding VP16 acti-vation domain of pGAVV was replaced with sequence encoding p65 activation domain residues 286–550, which was isolated from HEK293 cDNA by EcoRI and BsrGI. Site-directed mutagenesis, to generate sequences encoding VVD proteins with mutations C71V , N56K and C108S, was performed on sequence encoding the VVD domain according to the MutanBEST protocol (Takara). The reporter vector pU5-Gluc was generated by overlapping poly(A)–5×UASG -TATA sequence from pG5luc (Promega) and secreted Gluc sequence from pGLuc-basic (NEB), and subsequent ligation into NruI and BamHI sites of pcDNA3.1/Hygro(+) using CloneEZ PCR Cloning kit (Genescript), thereby replacing CMV promoter in pcDNA3.1/Hygro(+) (Invitrogen). The 2×Flag tag was added to C-terminal end of Gluc (added into the gene using BamHI and XbaI) for convenient immunoassay detection. Other reporter vectors including pU5-hrGFP , pU5-Fluc, pU5-mCherry and pU5-Insulin were generated by substituting Gluc with genes encoding humanized recombinant (hr)GFP , Fluc, mCherry and a minimal human proinsulin, respectively. Plasmid pU5-Gluc-ARE was constructed by inserting the ARE of the sequence encoding GM-CSF into 3′ untranslated region of Gluc . A furin consensus cleavage sequence was introduced to the minimal human proin-sulin gene, allowing the translational product to be constitutively processed and secreted in liver cells 20. Genes encoding hrGFP , Fluc, mCherry and insulin were introduced into HindIII and BamHI site of pcDNA3.1/Hygro(+) and Gluc was cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) to obtain CMV promoter–driven genes in expression vectors. To obtain the pU5-Cre vector, the multiple cloning site of pU5-Gluc was replaced, and Cre was introduced into HindIII-Eco47III site of the new vector by sub-stituting Gluc . To construct Escherichia coli expression vector, sequence encoding Gal4(65)-VVD was amplified from pGAVP and inserted into pET-28a(+) using CloneEZ PCR Cloning kit.Protein expression and purification. Gal4(65)-VVD was expressed in E. coli strain JM109 at 18 °C for 24 h under con-stant light in the presence of 0.4 mM IPTG, 10 µM ZnCl 2 and 5 µM FAD. The cell pellet was collected by centrifugation and sonicated in buffer A containing 20 mM Hepes, 0.5 M NaCl, 10 µM ZnCl 2, 20 mM imidazole, 10 mM β-mercaptoethanol and 10% glycerol, pH 7.5. The soluble cell lysate was fractionated by centrifugation. The supernatant was passed over a HisTrap FF column (GE Healthcare) and then washed thoroughly in buffer B containing 20 mM Hepes, 0.5 M NaCl, 50 µM ZnCl 2, 300 mM imidazole, 10 mM β-mercaptoethanol and 10% glycerol, pH 7.5. Proteins were desalted in 20 mM Hepes, 0.15 M NaCl, 20 µM ZnCl 2 and 10% glycerol, pH 7.5, using a HisTrap desalting column (GE Healthcare). After purification, Gal4(65)-VVD was stored at 4 °C and protected from light for recovery in the dark.Electrophoretic mobility shift assay. The probes used were as follows: 5′-TCTTCGGAGGGCTGTCACCCGAATATA-3′ and 5′-ACCGGAGGACAGTCCTCCGG-3′12. All samples containing VVD-derived proteins were prepared under red LED safe light. The DNA was annealed and diluted in 20 mM Hepes and 50 mM NaCl, pH 7.5 (renaturation buffer), to a final reaction DNA duplex concentration of 125 nM. Protein was diluted in renaturation buffer containing 100 µg ml −1 BSA (Jackson ImmunoResearch) by twofold serial dilution from 5.6 µM to 0.34 µM protein in dim red light. Protein and DNA were equilibrated at room tempera-ture (20–25 °C) for 30 min in reaction buffer with an additional5% (w/v) Ficoll either in the dark or with 15 W m −2 constant bluelight. After incubation, the dark and light irradiated samples were separately loaded onto different 6% native polyacrylamide gels in 0.5× Tris-borate buffer and were run at 100 V at 4 °C in the dark or with 15 W m −2 blue-light irradiance, respectively. After electrophoresis, the gel was stained with GelRed nucleic acid gel stain (Biotium) before fluorescence imaging using the In-Vivo Multispectral System FX (Kodak) with 530 nm excitation and 600 nm emission filters. Images of full-length gels from Figure 1b are available in Supplementary Figure 14.Cell culture and blue light irradiation. HEK293, HepG2, MDA-MB-231, MCF7 and PC-3 cells were maintained in high-glucose DMEM (HyClone) supplemented with 10% FBS, penicillin and streptomycin (Invitrogen). Cells were plated in phenol red–free, antibiotic-free high glucose DMEM supplemented with 10% FBS 16 h before transfection. We typically used equal amounts (0.4 µg each) of the light-switchable transactivator and reporter constructs with 2.4 µl Lipofectamine 2000 (Invitrogen) for each well of a 12-well plate according to the manufacturer’s protocol. To estimate the transcription efficiency of the LightOn system, equal amounts of CMV promoter–driven reporter constructs were used to trans-fect the cells as a control. Unless indicated, the transfected cells were kept in the dark for 10 h, and then they were illuminated by0.84 W m –2 (average irradiance) blue light from an LED lamp(460 nm peak) from below or remained in the dark for 22 h before characterization. The LED lamps were controlled with a timer to adjust the overall dose of blue light illumination during the speci-fied period (Supplementary Fig. 13a ). Neutral density filters were used to adjust the light irradiance. To spatially control gene expres-sion in cultured mammalian cells, single layers of HEK293 cells cultured on glass bottom dishes were transiently transfected with an mCherry reporter and the GA VPO transactivator, and then, the cells were illuminated with a spatial pattern using a photomask printed with a specific image for 24 h. LightOn system is sensi-tive to ambient light. One minute exposure to 0.16 W m −2 white fluorescent lamp light lead to substantial induction of gene expres-sion, whereas there was minimal gene induction when cells were illuminated with 630 nm red LED light. In this study, experiment procedures after cell transfection were carried out under red LED light, and cells were cultured inside dedicated CO 2 incubators.Animal experiments. All procedures involving animals were approved by the Institutional Animal Care and Use Committee of Shanghai and were conducted in accordance with the National Research Council Guide for Care and Use of Laboratory Animals. Unless otherwise mentioned, 10 µg of pGAVPO and 300 µg of pU5 vector carrying target gene were transferred into mice by。
Agilent SPME Fiber or Arrow Manual Injection Kit U
12345SPME Fiber or Arrow Manual Injection KitSPME manual samplingThe Agilent manual injection kit will allow the end user to extract samples using SPME fibers or Arrows. They can then inject the samples into a GC inlet.Manual SPME SamplingSPME fibers and Arrowsp/n 5191-58772PAL3 alignment ring (gray) for split/splitless (S/SL) inletManual injectionManual injection guidePAL3 alignment ring (Gray) for S/SL inlet (G7371-67001)The manual injection guide sits on thealignment ring for manual sample injection.3Methodology—manual samplingInstalling a PDMS SPME (100 μm) Arrow into the manual syringeLoosen the cap at the base of the syringe and remove it.Depress the black plunger completely.Screw the hub of an SPME fiber/Arrow into the bottom of the plunger at the end of thesyringe bodyRetract the black plunger and slide the cap over the SPME fiber/Arrow and tighten itonto the syringe.4The extraction guide has two positions where the syringe can be installed.The upper position is used for headspace extraction.The lower position is used for immersion extraction.Incorrect and correct position of the lower locking screw.Do not tighten the screw against the black plunger or you will not be able to move the SPME fiber/Arrow intoposition for sampling.Setting the locking screwsLarge inner diameter (id) locking screwSmall inner diameter (id) locking screwSlide the locking screws onto the syringe from the plunger side (the right side as shown above).• Install the large id locking screw onto the silver body of the syringe.• Install the small id locking screw onto the wider portion of the black plunger.•Tighten the locking screws until finger-tight. Do not overtighten, as they will be adjusted in later steps.5• Raise the syringe plunger to the fully extended position and insert the syringe and lower locking screw into the upper position of the extraction guide.•Lock the syringe into place by rotating it until the locking screw is positioned in the notch.• Adjust the syringe so that the SPME fiber/Arrow is protruding ~1 cm beyond the inner base of the extraction guide (A).• Tighten the lower locking screw securely.•The tip of the SPME fiber/Arrow will be recessed at least 1 mm in from the end ofthe extraction guide (B).A BSetting the locking screws for septum penetration depthPlace the extraction guide (with syringe in place) on a headspace sampling vial and loosen the upper locking screw.Adjust the SPME fiber/Arrow to the desired exposure depth by moving the black plunger.Choose a depth that ensures that the SPME fiber/Arrow will be in the gas phase.Once the SPME fiber/Arrow is at the proper depth, hold the plunger in place and slide the upper locking screw until it is flush against the top of the silver syringe body. Then tighten the upper locking screw securely.Setting the exposure depth for headspace extraction6Fine depth adjustment for direct immersion extractionAdjusting the injector penetration depthInsert the syringe into the lower position of the extraction guide.1. Manual SPME injection guide2. PAL3 alignment ring (gray) forS/SL inlet (G7371-67001)• Carefully insert the syringe into the injection guide.• Use caution to avoid damaging the SPME fiber/Arrow when threading it through the hole in the base of the injection guide.•Lock the syringe into place by rotating it until the locking screw is positioned in the notch.Penetrate a vial and fully expose the SPME fiber/Arrow within the vial.Adjust the lower locking screw and upper locking screw to obtain the desired exposure depth (to ensureimmersion in the sample liquid).127Setting injector penetration depthWith the appropriate GC-specific adaptor cup on the end of the injection guide, measure the distance from the tip of the SPME fiber/Arrow to the groove inside the adaptor cup.Adjust the desorption depth by screwing the body of the injection guide up or down (maximum depth = 67 mm).Twist the locking ring down until it locks on the body of the injection guide./chemDE.3985648148This information is subject to change without notice.© Agilent Technologies, Inc. 2020 Printed in the USA, March 6, 2020 5994-1732ENInjection onto the GC inletRemove the adapter cup from the injection guide.The adapter cup is placed onto the GC inlet to guide the manual injection.Push the plunger down until the top locking screw is resting on the body of the syringe.The sample is then injected.。
电力电气中英文——综合部分
2872.长度length2873.面积area2874.体积(容积)volume2875.质量mass2876.重量weight2877.能量energy2878.形状shape2879.方形square2880.圆形round2881.三角形triangle2882.半径radius2883.直径diameter2884.周长circumference, perimeter2885.参数parameter2886.工况operating condition2887.温度temperature2888.压力pressure2889.力矩moment of force2890.时间常数time constant2891.真空vacuum2892.流量flow, flow capacity2893.比热specific heat2894.比容specific volume2895.热流量heat flow2896.吸热heat absorption, thermal absorption 2897.吸热反应endothermic reaction2898.放热heat liberation, heat release 2899.压缩compression2900.膨胀expansion2901.节流throttle2902.正压positive pressure2903.负压negative pressure2904.起动start-up, starting2905.空转idle running2906.停机shutdown, outage, stoppage 2907.试验test2908.检测detection, test2909.并联parallel connection2910.串联series connection2911.过热overheat2912.再热reheat2913.回热regeneration2914.饱和蒸汽saturated steam2915.允许温度allowable temperature2916.干度dryness2917.水蒸汽steam, water vapor2918.焓enthalpy2919.熵entropy2920.郎肯循环(兰金循环)rankine cycle2921.卡诺循环carnot cycle2922.气体gas2923.液体liquid2924.固体solid2925.海拔altitude2926.标高elevation2927.规程rules, regulation2928.运行规程operation rules(regulations) 2929.工艺规程process rules(regulations)2930.检修规程maintenance rules(regulations) 2931.安全规程safety rules(regulations)2932.反事故措施anti-accident measures2933.防火措施fire-prevention measures2934.防爆措施explosion prevention measure 2935.维护maintenance2936.缺陷处理defect treatment2937.铭牌nameplate2938.合格证书passport, certificate2939.国际单位international unit2940.技术检验记录technical check record2941.技术资料technical information, technical data 2942.检修间repair shop, service shop2943.检修记录repair record, maintenance2944.检查性试验check-up test2945.日常检查daily inspection, routine inspection 2946.定期检查periodic repair2947.解体检查repair with disassembly2948.现场检修on-site repair2949.临时检修temporary repair, running repair 2950.试运行trial operation2951.事故accident, emergency2952.占地指标land allocation2953.绿化面积area of greenery space2954.绿化系数greenery factor, greening factor 2955.发电量power generation, generated energy 2956.装机容量installed capacity2957.额定转速rated speed2958.线损率line loss rate2959.热耗率(热耗)heat consumption rate2960.耗煤率(煤耗)coal consumption rate2961.供电标准耗煤率net standard coal consumption rate2962.发电标准耗煤率gross standard coal consumption rate2963.厂前区面积area of administrative zone2964.建筑系数building coverage2965.场地利用系数site utilization factor2966.百万千瓦耗水指标water consumption per GW2967.发电成本power generation cost2968.单位千瓦造价cost per KW2969.烟气排放标准flue gas emission standard2970.污水排放标准sewage discharge standard2971.职工定员personnel quota2972.运行可用率operation availability2973.运行可靠性operation reliability2974.利用小时数number of utilization hours2975.三材消耗率(钢/水泥/木材)consumption of steel, cement and timber 2976.生活福利区面积living and welfare facility zone area2977.年耗煤量annual coal consumption2978.小时耗煤量hourly coal consumption2979.单元机组联合试运行integral trial operation of unit plant2980.设备效率equipment efficiency2981.设备利用率equipment capacity factor2982.设备缺陷equipment defector2983.热备用状态hot reserve2984.冷备用状态cold reserve2985.设备备用equipment in reserve2986.紧急备用emergency reserve2987.停止备用stop standby2988.凝汽式电厂condensing power plant2989.热电厂cogeneration power plant2990.坑口电厂mine-mouth power plant2991.路口电厂railway-side power plant2992.港口电厂port power plant2993.核电站nuclear power plant2994.水电站hydropower plant(station)2995.抽水蓄能电站pumped storage power station2996.风力发电站wind power station2997.太阳能发电站solar power station2998.地热电站geothermal power station2999.柴油发电厂diesel generating power station3000.燃气蒸汽联合循环电厂steam and gas turbine combined-cycle power plant 3001.燃气轮机电厂gas turbine power plant3002.煤气化联合循环电厂integral gasified combined-cycle power plant3003.自备电站captive power plant3004.峰荷电站peak load power plant(station)3005.基荷电站base load power plant3006.新建电厂newly-built power plant3007.扩建工程extension project3008.技术改造工程technical renovation project3009.初步可行性研究preliminary feasibility study3010.概念设计conceptual design3011.预设计predesign3012.初步设计preliminary design3013.施工图设计detail design3014.施工组织设计construction organization design3015.设计审查design review, design examination3016.设计审批design examination and approval3017.设计回访feedback on design quality3018.计算书calculation sheet3019.技术规范书technical specifications3020.设计说明书design instructions3021.总工程师chief designer3022.设计总工程师project chief engineer3023.主要设计人chief designer3024.设计人designer3025.校核人checker3026.描图号tracer3027.晒图blueprint3028.复印copying3029.装订binding3030.出版publication3031.工地代表site resident representative3032.项目建议书project proposal3033.可研批复comments on and approve of feasibility study report 3034.中外合作设计sino-foreign cooperative design3035.设计联络会design liaison meeting3036.行业标准industrial standard3037.国家标准national standard3038.技术标准technical standard3039.项目咨询project consultation3040.设备选择equipment selection3041.建议规模construction scale3042.工程设想engineering projection3043.电力系统地理接线图geographical system connection diagram 3044.负荷测试load forecast3045.总平面布置图general layout3046.竖向布置图vertical layout drawing3047.厂区地下沟道图plant-area underground piping and culvertsdrawing3048.交通运输communication and transportation3049.煤源coal source3050.水源water source3051.接入系统connection to power system3052.经济效益分析economic benefit analysis3053.热力系统图thermodynamic system diagram3054.主厂房布置图main machine building arrangement drawing3055.燃烧系统图combustion system diagram3056.供水系统图water supply system diagram3057.除灰系统图ash handling system diagram3058.工业水处理系统industrial water system diagram3059.化学水处理系统图chemical water treatment system diagram3060.锅炉点火系统图boiler ignition system diagram3061.电气主接线图main wiring diagram, main electrical connection diagram 3062.单元系统图unit system diagram3063.一次系统图primary system diagram3064.二次系统secondary system diagram3065.励磁系统图excitation system diagram3066.输电电压等级transmission voltage grade(class)3067.回路数loop number3068.纵向布置longitudinal arrangement3069.横向布置transversal arrangement3070.室内布置indoor arrangement3071.露天布置outdoor arrangement, open-air arrangement3072.半露天布置semi-outdoor arrangement, semi-open-air arrangement 3073.环境影响评价报告environmental impact assessment report3074.推荐方案recommended proposal3075.优化方案optimum proposal3076.工程地质engineering geology3077.工程测量engineering survey3078.水文气象hydrometeorology3079.岩土工程rock and earth engineering3080.水文地质hydrogeology3081.物理勘探physical prospecting3082.航测aerial survey3083.地层结构stratum structure3084.地震烈度seismic intensity, earthquake intensity3085.地质断裂geologic fracture, geologic rift3086.地耐力earth bearing capacity3087.天然地基natural foundation3088.人工地基artificial foundation, artificial base3089.软土地基soft soil base3090.地基处理base treatment, foundation treatment3091.桩型选择pile type selection3092.打桩piling, pile driving3093.试桩trial piling3094.预制桩precast pile3095.钢板桩steel sheet pile3096.钢管桩steel pipe pile3097.夯实ramming ,tamping3098.地下水ground water, underground water3099.地表水surface water3100.泉水spring, spring water3101.储量reserve3102.迳流量run-off volume3103.勘测大纲survey outline3104.初步勘测preliminary survey3105.详细勘测detailed survey3106.最终勘测final survey3107.河势分析river potential analysis3108.航道waterway3109.主厂房main machine hall, main power building3110.汽机房turbine hall, turbine house3111.除氧间(除气间)deaerator bay3112.煤仓间bunker bay3113.锅炉房boiler hall, boiler house3114.烟囱chimney, stack3115.单管烟囱single-shaft chimney(stack)3116.多管烟囱multi-shaft chimney(stack)3117.脱硫装置desulphurization equipment3118.铁路专用线plant service railway line, special service railway line 3119.铁路专用线接轨点special service railway coupling point 3120.灰场ash yard3121.山谷灰场valley ash yard3122.渣场slag yard3123.灰坝ash dam3124.事故灰场emergency ash yard3125.近期near future, short-term3126.远期future, long-term3127.屋顶roof3128.屋架roof truss3129.屋架下弦标高roof truss lower chord elevation3130.天窗skylight3131.远转层operation floor3132.底层ground floor3133.皮带层belt conveyor floor3134.地下室basement3135.电缆沟cable trench3136.电缆隧道cable tunnel3137.集水坑water collection pit3138.梁beam3139.柱column, pillar3140.楼板floor slab3141.网格板grating plate3142.平台platform3143.扶梯stair3144.地面ground3145.牛腿bracket3146.下水道sewer3147.设备基础equipment foundation3148.抗震架构shockproof structure, aseis-matic structure 3149.跨度span3150.柱距column space3151.钢结构steel structure3152.钢筋混凝土结构reinforced concrete structure3153.预制构件precast parts, prefabricated parts3154.空心板hollow block3155.压型钢板channeled steel plate3156.沟盖板trench cover plate3157.防浪堤revetment3158.涵洞culvert3159.防火距离fire proof distance3160.装饰标准decoration standard3161.环境保护environmental protection3162.污水处理站sewage treatment station3163.干法脱硫dry desulphurization3164.湿法脱硫wet desulphurization3165.综合利用comprehensive utilization3166.环保检测站environmental monitoring3167.制氢站hydrogen generation station3168.制氧站oxygen generation station3169.变压器检修间transformer maintenance room3170.空压机间air compressor room3171.输煤综合楼coal handling complex building3172.网络控制楼grid control building, network control building 3173.生产办公楼plant service building3174.化学水处理间chemical water treatment room3175.修配厂repair workshop3176.油罐区oil tank zone3177.重油罐heavy oil tank3178.消防器材fire-fighting equipment3179.泡沫消防foam fire extinguishing3180.消防拴fire hyfrant3181.翻车机室wagon tipper house3182.推煤机库coal bulldozer house3183.煤场coal yard3184.干煤棚dry coal shed3185.混煤仓mixed coal silo3186.输煤皮带栈桥coal belt conveyor gallery3187.行政办公楼administrative building3188.传达室reception room3189.单身宿舍楼dormitory3190.食堂dining room, canteen3191.医院hospital3192.俱乐部club3193.礼堂auditorium, assembly hall3194.商店shop, store3195.码头wharf3196.煤码头coal unloading wharf, coal jetty3197.油码头oil unloading wharf3198.大件运输码头heavy goods wharf3199.冷却水塔cooling tower3200.水库reservoir3201.库容reservoir capacity3202.蓄水池water storage pond3203.最高洪水位maximum flood level3204.厂址标高site elevation3205.冲沟rush channel3206.渗漏leakage, seepage3207.排洪渠(沟)flood discharge channel3208.围墙enclosing wall, enclosure3209.主导风向(东、南、西、北)prevailing wind direction(east,west, south, north) 3210.平均风速average wind speed3211.最大风速maximum wind speed3212.上风向upwind direction3213.下风向downwind direction3214.城市规划city planning3215.采暖heating3216.空调air conditioning3217.照明lighting3218.日光灯fluorescent lamp3219.白炽灯incandescent lamp3220.通风ventilation3221.热交换器heat exchange3222.机械排风mechanical ventilation, mechanical 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thermal insulation3259.油漆paint3260.节能energy conservation, energy saving3261.节水water saving3262.节电power saving, power conservation3263.节约用电economizing land in use3264.灭火器fire extinguisher3265.泡沫灭火器foam fire extinguisher3266.土方开挖earth excavation3267.回填土backfilled earth3268.仿真机(模拟机)simulator3269.检修maintenance, repair3270.运行operation, running3271.涉外谈判negotiation with foreigners3272.独立自主maintaining independence and keeping the initiative in one’s own hands 3273.自力更生relying on one’s own efforts3274.沿海地区coastal areas3275.经济特区special economic zone3276.出口贸易export trade3277.商品commodity3278.出口额export volume3279.出口许可证export license3280.法人legal person3281.企业法人legal entity3282.法人代表representative of legal entity, representative of legal person3283.业主owner3284.超额累进税surtax3285.比例税proportional tax3286.承包工程contracting project3287.出口关税export duty3288.注册资本额registered capital amount3289.多边支援multilateral assistance3290.专利权patent, patent right3291.商标权trademark right3292.固定资产fixed assets3293.工业产权industrial property3294.资源开发resources development, resources exploitation3295.子公司subsidiary company, branch company3296.母公司holding company, parent company3297.国际债券international bond3298.生产线production line3299.流动资金current capital, current fund3300.托收collection3301.银行信贷bank credit3302.海运提单ocean bill of lading, ocean waybill3303.签定合同contract signing3304.备用信用证standby letter of credit3305.条款terms and conditions3306.询价inquiry3307.顺汇favorable exchange3308.逆汇reverse remittance3309.相对免赔率franchise3310.汇票draft3311.共同海损general average3312.离岸品质shipping quality3313.到岸品质arrival quality3314.国际投资银行international investment Bank3315.国际金融公司international finance Corporation3316.劳务贸易service trade3317.银团贷款syndicated loan3318.项目选定project identification3319.项目准备project preparation3320.项目评估project appraisal3321.项目谈判project negotiation3322.项目执行与监督project implementation and supervision 3323.项目后评估project evaluation3324.出口信贷export credit3325.利息期interest period3326.软贷款soft loan3327.硬贷款hard loan3328.合同contract3329.混合贷款blend3330.协议agreement3331.意向书letter of intent3332.项目经济评价project economic evaluation3333.资金筹措fund raising3334.比例分配proportional distribution3335.股份share3336.董事会board of directors3337.董事长chairman of the board3338.总经理president, general manager3339.易货贸易barter trade, barter3340.集资fund collection3341.融资financing3342.外汇偿还能力foreign exchange repaying ability 3343.设备费equipment3344.价格price3345.人工费labour cost3346.征地价格land acquisition price3347.进口范围import scope3348.国产设备domestic manufactured equipment3349.建设期贷款利率interest rate during construction 3350.流动资金贷款利率current fund loan interest rate 3351.工程投资估算project cost estimate3352.工程投资概算project budgetary estinate3353.工程投资决算final accounts of project cost3354.调整概算budgetary estimate adjustment3355.工程投资预算project budget3356.设备验收equipment acceptance3357.国家开发银行state development bank3358.商业贷款commercial loan3359.经济用语economic expressions3360.投资回收年限investment recovery period3361.总投资total investment3362.注册资本registered capital3363.信贷资本credit proceeds3364.敏感性分析sensitive analysis3365.电价electricity price, electricity tariff3366.煤价coal price, coal tariff3367.汇率exchange rate3368.静态static status3369.动态dynamic status3370.金融finance3371.资本capital3372.离岸价格FOB price (free on board)3373.到岸价格CIF price (cost, insurance, freight) 3374.运输费freight3375.风险费risk premium3376.保险金insurance premium3377.签字仪式signing ceremony3378.招商inviting businessmen to invest3379.租赁lease3380.证券securities3381.税率taxation rate3382.经营management3383.盈利profiting3384.亏损lossing3385.效益benefit3386.货币currency3387.售电价格electricity selling price3388.市场经济market economy3389.市场竞争market competition3390.计划经济planned economy3391.独立核算independent accounting3392.中外合资企业Sino-foreign joint venture enterprise 3393.固定资产所有权ownership of fixed assets 3394.建议银行construction bank3395.亚洲开发银行Asian development bank3396.世界银行world bank3397.招标inviting bids3398.投标submitting a bid, submitting a tender3399.投标人bidder3400.投标书bid, tender3401.中标人bid winner, successful bidder3402.招标文件bidding document s3403.投标价格bid price3404.售标selling bidding document3405.评标bid evaluation3406.废标bid rejection3407.国际竞争式招标international competitive bidding 3408.国内竞争式招标local competitive bidding3409.有限国内招标limited international bidding 3410.授予合同award of contract3411.投标书有效期validity period of bid3412.投标保证金bid security3413.资格预审prequalification3414.资格后审post qualification3415.标书澄清clarification of bidding document 3416.标书修改amendment of bidding document 3417.重新招标rebidding2418.国务院state council2419.国家计委state planning commission2420.国家经贸委state economic and trade commission 2421.财政部ministry of finance2422.劳动部ministry of labour2423.电力工业部ministry of electric power industry 2424.煤炭工业部ministry of coal industry2425.铁道部ministry of railway2426.省人民政府provin cial people’s government 2427.省计委provincial planning commission2428.省建委provincial construction commission 2429.公安局public security bureau2430.国家土地管理局state land administration3431.规划局planning bureau3432.劳动局labour bureau3433.环保局environmental protection bureau3434.水利局water conservation bureau3435.地震局seismological bureau3436.铁路局railway administration,3437.公路局highway administration, highway bureau 3438.港务局port administration, port authority3439.城建设计院urban construction design institute 3440.航道设计院navigation-way administration 3441.铁道设计院railway design institute3442.电力集团公司power group corporation3443.省电力局provincial electric power bureau3444.供电局power supply bureau3445.电力设计院electric power design institute3446.变电所substation3447.电厂筹建处power plant preparatory office3448.华能国际电力开发公司Huaneng international power development corporation 3449.华能发电公司Huaneng power generation corporation3450.华能集团Huaneng group3451.厂长室plant director office3452.总工室chief-engineer office3453.厂办公室plant general office3454.生技科production technology section3455.安监科safety supervision section3456.科技科science and technology section3457.计划科planning section3458.财务科finance section3459.计算机开发公司computer development company3460.技术服务公司thchnology service company3461.老干部退休办公室retired cadres administration office3462.汽机分场turbine department3463.电气分场electrical department3464.热工分场control and automation department3465.化学分场chemical department3466.修配分场repair and supply workshop3467.修缮分场repair shop3468.通讯分场communication department3469.发电分场power generation department3470.燃料处fuel disation3471.部长minister3472.局长department director3473.厂长plant direction3474.生产副厂长vice director in charge of production3476.运行副总工程师vice chief-engineer in charge of operation3477.检修副总工程师vice chief- engineer in charge of maintenance3478.值长shift chief-operator3479.班长group chief, team chief3480.科长section chief3481.副科长vice section chief3482.主任director3483.副主任vice director3484.单元控制室unit control room3485.网络控制室network control room3486.计算机室computer room3487.安全运行天数number of safe operation days3488.文明生产civilizer production3489.司机driver3490.助手assistant3491.给水值班员attendant of feed pump3492.辅机值班员attendant of auxiliary equipment 3493.岗位post3494.岗位专责post responsibility。
NOVEL LIGHT SOURCES AND METHODS FOR ILLUMINATING P
专利名称:NOVEL LIGHT SOURCES AND METHODS FORILLUMINATING PLANTS TO ACHIEVEEFFECTIVE PLANT GROWTH发明人:John Goeschl申请号:US13490184申请日:20120606公开号:US20130329417A1公开日:20131212专利内容由知识产权出版社提供专利附图:摘要:A light source for growing a plant or its propagule is described. The light source includes: (i) a power component requiring an input power; (ii) a light source receiving theinput power to illuminate, for a period of time, a growth area of the plant/propagule with an incident light having a spectral profile defined by a first (i.e., between about 400 nm and about 470 nm), a second (i.e., between about 526 nm and about 570 nm) and a third (i.e., between about 626 nm and about 700 nm) set of wavelengths, the light source component provides a photosynthetic productivity from the plant/propagule that is greater than that achieved if the same the growth area of the plant/propagule had been illuminated by another incident light for substantially same time period, and the another incident light includes the first and third, but not the second set of wavelengths.申请人:John Goeschl地址:Davis CA US国籍:US更多信息请下载全文后查看。
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》一、引言随着现代生物医学的飞速发展,荧光探针技术在细胞成像、药物传递和生物检测等领域的应用越来越广泛。
近红外荧光探针,尤其是吸收波长大于700nm的荧光材料,因其在生物组织中具有较低的光散射和自体荧光干扰,成为当前研究的热点。
本文旨在研发双通道近红外pH荧光探针,并探讨非对称aza-BODIPY的合成及其在荧光探针中的应用。
二、双通道近红外pH荧光探针的研发背景与意义双通道近红外pH荧光探针的研发对于生物医学研究具有重要意义。
pH值是细胞内环境的重要参数之一,与许多生物过程密切相关。
通过双通道近红外荧光探针,可以实时监测细胞内pH 值的变化,为研究细胞生理和病理过程提供有力工具。
此外,近红外波段的荧光探针在生物成像中具有更好的穿透深度和组织分辨率。
三、非对称aza-BODIPY的合成及其性质研究1. 合成方法:非对称aza-BODIPY是一种新型的近红外荧光染料,其合成主要基于已知的BODIPY合成方法,通过引入不同的取代基和空间结构调整,实现非对称性的构建。
主要步骤包括多步有机合成和金属化反应。
2. 性质研究:合成的非对称aza-BODIPY具有较高的摩尔吸光系数和良好的光稳定性。
其吸收波长大于700nm,属于近红外区域,符合生物成像的需求。
此外,该染料还具有较高的荧光量子产率和较低的细胞毒性。
四、双通道近红外pH荧光探针的设计与制备1. 设计思路:双通道近红外pH荧光探针的设计基于非对称aza-BODIPY的优异性能。
通过引入pH敏感的基团,构建两个不同响应范围的荧光信号通道,以实现更精确的pH值检测。
2. 制备过程:制备过程包括选择合适的pH敏感基团、与非对称aza-BODIPY进行化学连接、优化探针的分子结构等步骤。
通过调整化学结构和反应条件,得到性能稳定的双通道近红外pH荧光探针。
五、实验结果与讨论1. 实验结果:通过合成和优化,成功制备了非对称aza-BODIPY及双通道近红外pH荧光探针。
稳态瞬态荧光光谱仪在荧光探针开发上的应用
稳态瞬态荧光光谱仪在荧光探针开发上的应用下载温馨提示:该文档是我店铺精心编制而成,希望大家下载以后,能够帮助大家解决实际的问题。
文档下载后可定制随意修改,请根据实际需要进行相应的调整和使用,谢谢!并且,本店铺为大家提供各种各样类型的实用资料,如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等,如想了解不同资料格式和写法,敬请关注!Download tips: This document is carefully compiled by theeditor.I hope that after you download them,they can help yousolve practical problems. The document can be customized andmodified after downloading,please adjust and use it according toactual needs, thank you!In addition, our shop provides you with various types ofpractical materials,such as educational essays, diaryappreciation,sentence excerpts,ancient poems,classic articles,topic composition,work summary,word parsing,copy excerpts,other materials and so on,want to know different data formats andwriting methods,please pay attention!稳态瞬态荧光光谱仪在荧光探针开发中的核心作用在现代科学研究中,荧光探针是一种不可或缺的工具,广泛用于生物学、化学、医学和环境科学等领域,它们能够通过荧光信号的变化来揭示特定分子事件或环境条件。
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》一、引言随着生物医学和生物传感技术的快速发展,荧光探针因其高灵敏度、非侵入性和实时监测等优势,在生物成像、药物传递、疾病诊断等领域得到了广泛应用。
其中,近红外荧光探针因其具有较低的生物自体荧光干扰、更深的组织穿透能力及较长的光漂白时间等特点,近年来成为了研究热点。
双通道荧光探针可以提供更丰富的信息,有利于实现复杂环境中更精确的检测和定位。
非对称Aza-BODIPY作为一种新型荧光染料,其独特的化学结构和光学性质使其在近红外荧光探针的研发中具有巨大潜力。
二、双通道近红外pH荧光探针的研发1. 设计思路双通道近红外pH荧光探针的设计需基于可逆pH响应的荧光基团,并确保其在不同pH环境下展现出明显的光谱变化。
设计过程中,应充分考虑分子的电子结构和能级,确保吸收和发射波长落在近红外区域。
2. 合成路径合成路径需选择合适的原料和反应条件,通过多步有机合成实现探针的构建。
关键步骤包括Aza-BODIPY核心结构的合成、pH响应基团的引入以及双通道荧光基团的偶联等。
3. 性能测试性能测试包括光谱分析、灵敏度检测及响应时间测定等。
利用紫外-可见-近红外分光光度计、荧光光谱仪等设备对探针的光学性质进行全面评价。
三、吸收波长大于700nm的非对称Aza-BODIPY的研制1. 结构设计非对称Aza-BODIPY的设计需考虑其共轭结构、电子分布及光学性质。
通过调整取代基的位置和类型,实现对其吸收波长的调控,使其大于700nm。
2. 合成与表征采用合适的合成方法制备非对称Aza-BODIPY,并通过核磁共振、质谱等手段对其结构进行表征,确保其纯度和结构正确性。
3. 光学性质研究研究非对称Aza-BODIPY的光谱性质,包括吸收光谱、发射光谱及量子产率等。
通过比较不同结构染料的性能,优化其光学性质。
四、实验结果与讨论1. 双通道近红外pH荧光探针的实验结果对合成的双通道近红外pH荧光探针进行光谱测试,分析其在不同pH环境下的光谱变化,评估其灵敏度和响应速度。
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》
《双通道近红外pH荧光探针的研发及吸收波长大于700nm的非对称aza-BODIPY的研制》一、引言随着生物医学技术的不断发展,荧光探针在生物成像、药物传递和疾病诊断等领域的应用日益广泛。
其中,近红外pH荧光探针因其对生物组织具有较低的光损伤和较强的穿透能力,成为研究的热点。
本文旨在研发一种双通道近红外pH荧光探针,并针对非对称Aza-BODIPY的合成进行深入研究。
二、双通道近红外pH荧光探针的研发背景及意义近红外荧光探针因其独特的优势在生物医学领域具有广泛的应用前景。
双通道近红外pH荧光探针能够同时监测多个生理参数,如pH值和特定离子的浓度,对于研究细胞内环境和疾病发展具有重要意义。
此外,该类探针的研发也有助于提高生物成像的准确性和灵敏度。
三、非对称Aza-BODIPY的合成与性质研究(一)非对称Aza-BODIPY的合成非对称Aza-BODIPY作为一种重要的荧光染料,其合成是研发双通道近红外pH荧光探针的关键步骤。
通过选择合适的反应条件和原料,采用逐步合成的策略,成功合成出非对称Aza-BODIPY。
(二)非对称Aza-BODIPY的性质研究合成的非对称Aza-BODIPY具有较高的荧光量子产率和良好的光稳定性。
其吸收波长大于700nm,符合近红外区域的荧光探针需求。
此外,该化合物还具有pH响应性,能够在不同pH环境下发出不同强度的荧光。
四、双通道近红外pH荧光探针的设计与制备(一)探针设计思路基于非对称Aza-BODIPY的优良性质,设计了一种双通道近红外pH荧光探针。
该探针包含两个功能部分:一个用于检测pH 值的响应基团和一个用于发出荧光的非对称Aza-BODIPY基团。
(二)制备方法通过化学键合将响应基团与非对称Aza-BODIPY基团连接在一起,制备出双通道近红外pH荧光探针。
该探针具有良好的水溶性,易于与生物分子结合,适用于生物成像和疾病诊断等领域。
五、实验结果与讨论(一)非对称Aza-BODIPY的合成与表征通过核磁共振、紫外-可见吸收光谱和荧光光谱等手段对合成的非对称Aza-BODIPY进行表征。
light of conversion harpia瞬态光谱
light of conversion harpia瞬态光谱
“Light of Conversion harpia瞬态光谱”可能指的是Light Conversion公司的HARPIA-TA型瞬态吸收光谱仪系统。
该系统能够提供泵浦-探测测量功能,具有多种探测光配置和检测选项,包括单波长探测的光电二极管,以及与光谱分辨宽带检测结合使用的白光超连续谱探针。
该系统具有广泛的自动化选项,提供泵浦和探测光束位置跟踪和对齐、泵浦偏振控制、超连续谱发生器切换、样品定位,以及瞬态吸收和瞬态反射测量之间的切换。
瞬态吸收光谱仪主要用于研究瞬态吸收动力学,当与PHAROS或CARBIDE激光器和ORPHEUS系列OPA一起使用时,最高重复率可达1MHz,从而可以在激发脉冲能量低至几纳焦的情况下进行研究。
该系统兼容低温恒温器和蠕动泵,并且可以使用扩展模块来扩展其功能。
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Light sources generating far fieldswith tunable flat profilesSerkan Sahin and Olga Korotkova*Department of Physics,University of Miami,1320Campo Sano Drive,Coral Gables,Florida33146,USA*Corresponding author:korotkova@Received March26,2012;revised May28,2012;accepted May29,2012;posted May30,2012(Doc.ID165410);published July13,2012Planar,scalar,optical Schell-model,and quasi-homogeneous sources with correlations that are Fourier transformsof multi-Gaussian functions are introduced.It is demonstrated that far fields produced by these families of sourcescarry interesting characteristics,being flatlike with adjustable steepness of the edge.Beam conditions for suchsources are also derived.©2012Optical Society of AmericaOCIS codes:030.1640,030.6600,260.0260.It is well known in statistical optics that the structure ofthe correlation function of a field in the source plane isclosely related to the intensity distribution of its far field([1],p.236).Moreover,for some model sources,it is pos-sible to obtain analytic relations between these two quan-tities.For instance,for quasi-homogeneous sources theso-called reciprocity relations are known to be of Fouriertype([1],p.243).For model sources of the Schell type[2],on the other hand,it is possible to obtain formulas for themajor statistical characteristics of the field propagatingto any distance from the source and to analyze howsource correlations gradually affect the field([1],p.276).Surprisingly few analytical models for the planarsource correlation functions have been developed so far:the Gaussian Schell-model(GSM)sources,the J n-Bessel-correlated sources[3],the I n-Bessel-correlated sources[4],the nonuniform GSM sources[5],and the Lambertiansources([1],p.248)might constitute the full list of mod-els.The difficulty in developing new models stems fromthe fact that a genuine correlation function must satisfy anumber of restrictions([1],Subsection4.7.1).To alleviatethis task,a sufficient condition in a simple integral formfor the genuine correlation function was derived in[6](see also[7])and has already led to a model in[5].In this Letter we introduce another model for the cor-relation function of a planar source that is based on themulti-Gaussian family of functions.Such functions havepreviously been employed for modeling of beams[8]andscattering potentials[9].Generally,the multi-Gaussianfunctions make it possible to control the width of the flatcenter of the beam intensity profile and the slope of itsedge by the choice of two parameters.But being employedfor modeling of the correlation,rather than for the field it-self,the multi-Gaussian function can serve as an importanttool for generating far fields with flat intensity profiles.Let us set the spectral degree of coherenceμ ρ1;ρ2;ωat a pair of points in the source plane with position vec-torsρ1andρ2and at the oscillation angular frequencyωin the form(in the following the dependence of all fieldstatistics on the angular frequencyωis implied butomitted for brevity)μ ρ1;ρ2;ω 1C0X Mm 1Mm−1 m−1m exp−jρ2−ρ1j22;(1)where C0PMm 1−1 m−1mMmis the normalization factor,Mmstand for binomial coefficients,andδis a typical cor-relation width.As illustrated in Fig.1,the profile functiondefined by Eq.(1)visually resembles a Bessel-correlatedsource or a Lambertian source(see[1],Figs.5.9and5.10);however,it is given by a different functional form.As in the case of a Bessel function whose Taylor’s expan-sion is a sum of sign-alternating terms,the modifiedmulti-Gaussian function is also represented by a sumof positive and negative exponentials.As is evident from[1,6],not any degree of coherencedefines a physically meaningful random source.For in-stance,for a Schell-model source,for which the cross-spectral density function has the formW 0 ρ1;ρ2S ρ1pS ρ2pμ ρ2−ρ1 ;(2)S ρ being the spectral density atρ,the sufficient condi-tion for the cross-spectral density to be genuine is that itmust be expressed by the integralW 0 ρ1;ρ2Zp υ H ρ1;υ H ρ2;υ d2υ;(3)- 4- 1140.810.60.40.2µ|ρ −ρ |δ12Fig.1.(Color online)Illustration of the degree of coherencecalculated from Eq.(1)as a function of the nondimensionalparameter jρ2−ρ1j∕δfor several values of M:M 1(solidcurve);M 4(dashed curve);M 10(dotted curve),andM 40(dotted–dashed curve).2970OPTICS LETTERS/Vol.37,No.14/July15,20120146-9592/12/142970-03$15.00/0©2012Optical Society of Americawhere H ρ;υ is an arbitrary kernel and p υ is a non-negative,Fourier-transformable function.Following [6]we assume that function H ρ;υ has the formH ρ;υ τ ρ exp −i υ·ρ ;(4)and hence W 0 becomesW 0 ρ1;ρ2 τ ρ1 τ ρ2 ~pρ1−ρ2 ;(5)where τ ρ is a (possibly complex)profile function and the tilde denotes the Fourier transform.The choice of p υ defines a family of sources with dif-ferent correlation functions.On taking the Fourier trans-form of Eq.(1)we arrive atp υδ20X M m 1 −1 m −1 Mm exp −m δ2j υj 2 ;(6)representing a family of flat-top profiles (see Fig.2).While it is a Fourier-transformable function by con-struction,its nonnegativity can be proven as follows.Equation (6)can be represented asp υ −δ2C 0XM m 1 M m−x mδ21− 1−x M ;(7)where x exp −δ2j υj 2∕2 .Function p υ is manifestly nonnegative since 0≤x ≤1.We point out that this form of p υ is particularly simple,since it is clear that 1−x is a saturation function whose slope is controlled by index M .Let us also set the Gaussian profile for function τ:τ ρ exp −j ρj 2∕ 4σ2 :(8)Then,together with the weighting function p υ given by Eq.(6),we obtain on substituting them into Eq.(5)the cross-spectral density function of the formW 0 ρ1;ρ2 10exp −j ρ1j 2 j ρ2j 2×X M m 1 −1 m −1m M m exp −j ρ2−ρ1j 22 ;(9)which may be called multi-Gaussian Schell-model source.We will now determine the cross-spectral density func-tion of the field radiated by the source Eq.(9)to the far zone at two points specified by position vectors r 1 r 1s 1and r 2 r 2s 2,with s 21 s 22 1.The field in the far zone of the source is given by the expression ([1],p.240)W ∞ r 1s 1;r 2s 2 2πk 2cos θ1cos θ2×~W 0 −k s 1⊥;k s 2⊥exp ik r 2−r 1 r 1r 2;(10)where k 2π∕λis the wavenumber of the field,λbeing the wavelength,s ⊥is the projection of s onto the source plane,s ⊥ sin θ,s z cos θ,and~W 0 f 1;f 2 14Z Z W 0 ρ1;ρ2 ×exp −i f 1·ρ1 f 2·ρ2 d 2ρ1d 2ρ2(11)is the four-dimensional Fourier transform.On substitut-ing Eq.(9)first into Eq.(11)and then into Eq.(10),weobtain for the cross-spectral density W ∞ r 1s 1;r 2s 2 in the far field the formula W ∞ r 1;r 21C 0k 2cos θ1cos θ2exp ik r 2−r 1 r 1r 2×X M m 1−1 m −1 M m 1a m −b m ×exp −k 2 αm s 21⊥ αm s 22⊥−2βm s 1⊥·s 2⊥ ;(12)wherea m 1 1212;b m 12;(13)αma m2m 2m ;βmb m2m 2m :(14)The far-field spectral density can be found by the formulaS ∞ r W ∞ r ;r ,and one obtains S ∞ rk 2cos 2θC 0j r j 2×X M m 1−1 m −1 M m exp −2k 2s 2⊥ αm−βm 2m 2m :(15)In order for the function W 0 ρ1;ρ2 to generate abeam,the spectral density in Eq.(15)must be negligible-4-2240.810.60.40.2δ|υ|pFig.2.(Color online)Illustration of the function p calculated from Eq.(6)as a function of nondimensional parameter δj υj for the same values of M as in Fig.1.July 15,2012/Vol.37,No.14/OPTICS LETTERS 2971except for directions within a narrow solid angle about the z axis.This is so if ([1],Eq.[5.6-72])exp −2k 2s 2⊥θ2 αm −βm ≈0(16)for any m 1;…;M ,unless s 2⊥≪1,implying that 2k 2 αm −βm ≪1;17 or,in terms of the source parameters,12 112≪2π22;m 1;…;M:(18)If the inequality holds for m 1,the rest of the set,for m 2;…;M holds automatically.Thus,the beam condi-tion for the multi-Gaussian Schell-model sources is the same as that for the GSM sources ([1],Eq.5.6-73):1 1≪2π2:19Figure 3shows several typical far fields radiated by thesource in Eq.(9).It is clearly seen that a beam with a Gaussian degree of coherence (M 1)in the source plane monotonically decreases with the increase of angle θ,while beams with multi-Gaussian source correlations have flat profiles with different heights and steepness of the edges.Another possible class of sources with the correlation function of the form Eq.(1)can be introduced with thehelp of a quasi-homogeneous ly,the cross-spectral density of such a source is of the formWρ1;ρ2 ≈Sρ1 ρ2μ ρ1−ρ2 ≈Sρ1 ρ22~p ρ1−ρ2 ;(20)under the assumption that function S is a slow function of its argument compared to μ.For this type of field,the far-field spectral density is given by ([1],p.243)S ∞ r12 2πk 2cos 2θ~F 0 ~μ k s ⊥ :(21)We also note that,since the multi-Gaussian Schell-model and multi-Gaussian quasi-homogeneous sourceshave the same correlation function of type Eq.(1),their far fields are qualitatively similar for M >1,especially if δ≪σ.In conclusion,we have introduced a family of func-tions whose Fourier transforms are multi-Gaussian functions for the source degree of coherence and have employed them in the Schell-model and the quasi-homogeneous model.We have confirmed that such sources are physically genuine,determined the far fields they generate,and showed that far fields have desirable features representing flat profiles that are useful in beam shaping,optical trapping,and tweezers.The novel family of correlation functions,while being visually similar to J n -Bessel correlations,result in qualitatively different far-field patterns,possessing a flat intensity profile in the central part with a steep,adjustable drop at its edge.We are indebted to Professor E.Shchepakina and the reviewer for their help in preparation of this Letter.O.Korotkova ’s research is supported by United States Office of Naval Research grant N00189-12-T-0136.References1.L.Mandel and E.Wolf,Optical Coherence and Quantum Optics (Cambridge University,1995).2.A.C.Schell,“The multiple plate antenna,”doctoral disser-tation (MIT,1961),Subsection 7.5.3.F.Gori,G.Guattari,and C.Padovani,mun.64,311(1987).4.S.A.Ponomarenko,J.Opt.Soc.Am.A 18,150(2001).junen and T.Saastamoinen,Opt.Lett.36,4104(2011).6.F.Gori and M.Santarsiero,Opt.Lett.32,3531(2007).7.R.Martinez-Herrero,P.M.Mejias,and F.Gori,Opt.Lett.34,1399(2009).8.F.Gori,mun.107,335(1994).9.S.Sahin,G.Gbur,and O.Korotkova,Opt.Lett.36,3957(2011).-0.004-0.0020.0020.00410.80.60.40.2θ [rad ]S (θ)οο( )Fig.3.(Color online)Far-field spectral density generated by a typical multi-Gaussian Schell-model source,calculated from Eq.(15),as a function of angle θ(in radians),for the same four values of M as in Fig.1and λ 632nm,σ 1mm,δ 0.1mm.2972OPTICS LETTERS /Vol.37,No.14/July 15,2012。