简单节杆菌groEL基因表达对大肠杆菌抗逆性能的影响

简单节杆菌groEL基因表达对大肠杆菌抗逆性能的影响
王艳霞;王红玲;王敏;骆健美
【期刊名称】《生物技术通报》
【年(卷),期】2016(032)005
【摘要】Arthrobacter simplex,a strain with steroid C1,2 dehydrogenation ability,has been widely used in the steroid industry, usually adding ethanol in the transforming system may catalyze the dissolution of hydrophobic substrates. Our prior work by LC-MS/MS and bioinformatics analysis found that heat shock protein(HSP)GroEL was closely correlated to the cell adaptive behavior under ethanol stress condition. Based on this concept,groEL gene was cloned by PCR while usingA. simplexTCCC 11037 genome as a template. The results showed that the homologies of nucleotide and amino acid sequence of GroEL in the strain were the highest with that fromNocardioides sp. JS614 by the ratio of 89% and 95%,respectively. Then its heterologous expression was conducted in Escherichia coli BL21(DE3)with the vector pET-22b (+),and the results showed that the recombinant strain presented the significant increase on the survival performance under the shock condition with high-concentration of methanol,hypertonicity and superacid,as well as the growth performance under the proper stresses of them. The study was the first report on the cloning and expression of genegroEL fromA. simplex,and also provided fundamental data for exploring the biological
function of HSP protein GroEL from A. simplex.%以简单节杆菌（Arthrobacter simplex）TCCC 11037基因组为模板，通过PCR方法进行groEL基因克隆，结果表明，该菌株中GroEL的核苷酸和氨基酸序列与Nocardioidessp. JS614来源的GroEL同源性最高，分别为89%和95%。

通过pET-22b（+）将其在大肠杆菌BL21（DE3）中进行异源表达，结果表明，重组
菌株在甲醇、高渗和强酸这3种压力高浓度冲击条件下的存活能力和适当压力条
件下的生长性能均明显提高。

【总页数】7页(P180-186)
【作者】王艳霞;王红玲;王敏;骆健美
【作者单位】天津科技大学生物工程学院工业发酵微生物教育部重点实验室天津科技大学天津市工业微生物重点实验室，天津 300457;天津科技大学生物工程学
院工业发酵微生物教育部重点实验室天津科技大学天津市工业微生物重点实验室，天津 300457;天津科技大学生物工程学院工业发酵微生物教育部重点实验室天津科技大学天津市工业微生物重点实验室，天津 300457;天津科技大学生物工程学
院工业发酵微生物教育部重点实验室天津科技大学天津市工业微生物重点实验室，天津 300457
【正文语种】中文
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