赤霉素对盐胁迫下红砂种子萌发的影响

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第26卷第6期Vol.26,No. 6

草业学报

八CTA PR八丁八C U L T U R八E SIN IC八

89-97

2017年6月

DOI:10. 11686/cyxb2016288 http: //cyxb. lzu. edu. cn 牛宋芳,王利娟,刘秉儒.赤霉素对盐胁迫下红砂种子萌发的影响.草业学报,2017, 26(6): 89-97.

NIU Song-Fang, WANG Li-Juan» LIU Bing-Ru. Effects of gibberellin on the germination of R e a u m u r i a soongorica seeds under salt stress. Acta Prataculturae Sinica, 2017,26(6) :89-97.

赤霉素对盐胁迫下红砂种子萌发的影响

牛宋芳〃,王利娟〃,刘秉儒1>2*

(1.宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,宁夏银川750021; 2.宁夏大学

西北退化生态系统恢复与重建教育部重点实验室,宁夏银川750021)

摘要:超旱生小灌木红砂是干旱半干旱区优势乡土物种,但是种子依靠萌发繁殖活力较低,研究赤霉素与不同浓度

盐交互作用对红砂种子萌发的影响,对于植被恢复和改善我国西部的生态环境具有重要作用。本试验采用培养皿

纸上发芽法,对红砂种子进行不同赤霉素浓度(1〇〇、200、400、600 m g/L)、盐浓度(50、100、150、200、250、300

m m ol/L)以及赤霉素与盐交互作用3种处理,以蒸馏水处理为对照,研究赤霉素对盐胁迫后红砂种子萌发和幼苗

生长情况的影响。结果表明:盐胁迫抑制种子的萌发,红砂种子的发芽率随盐浓度升高逐渐下降,发芽整齐度随浓

度升高而逐渐增大,胚根长与盐浓度具有显著负相关关系O?2=0.9452);使用较高浓度的赤霉素处理红砂种子,发

芽率、发芽指数与发芽速度均随浓度增大而先减小后增大的趋势,阈值为200 m g/L,不同浓度赤霉素处理后的红

砂种子的胚根长及胚芽长之间无显著性差异;赤霉素对较高浓度(>1〇〇m m ol/L)盐胁迫后红砂种子的发芽率有显

著的改善作用,可以提高种子的发芽速度、发芽指数。赤霉素能促进盐胁迫后红砂的种子萌发,促进胚的发育。

关键词:红砂;赤霉素;盐胁迫;发芽率

Effects of gibberellin on the germination of R eaum uria soon gorica seeds under salt stress

N IU Song-Fang1,2,W ANG Li-Juan1,2,LIU Bing-Ru1,2*

1.B reeding Base fo r State K ey Laboratory o f L and D egradation and Ecological Restoration o f N orth-w estern China,N in g x ia U­niversity ■,Yinchuan750021, China',

2. K ey bab fo r Restoration and Reconstruction o f D egraded Ecosystem in N orth-w estern China o f M in istry o f Education, N in g x ia U n iversity,Yinchuan750021, China

A bstract:R ea u m u r ia soon gorica , an extrem e xeric shrub and a dom inant species in desert and sem i-desert are­a s, has low levels of reproductive through germ ination. T his shrub could be an im portant species for vegetation restoration and the im provem ent of eco-environm ents in w estern China. A study has been undertaken to inves­tigate the effect of interactions betw een gibberellin (G A) and different salinity conditions on seed germ ination in R. so ongorica.U sing petri dish techniques, the treatm ents studied w ere germ ination under H20(c o n tro l), various concentrations of NaCl to explore saline stress (100, 200, 400, 600 m g/L;treated group 1) , a range of GA concentrations (50, 100, 150, 200, 250, 300 m m o l/L, treated group 2) and their com bined interaction (treated group 3). T he results showed that seed germ ination percentages continuously decreased w ith increas­ing salinity, while germ ination uniform ity increased w ith rising salt levels. W ith increasing NaCl concentra-

收稿日期:2016-07-18 ;改回日期:2016-09-28

基金项目:宁夏自然科学基金(NZ15001)资助…

作者简介:牛宋芳(1992-),女,安徽阜阳人,在读硕士… E-mail:983557664@qq. com

* 通信作者Corresponding author. E-mail : bingru. liu@163. com

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