甲状旁腺激素对老年大鼠种植体骨结合的影响及机制研究
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关键词：牙种植，骨内；甲状旁腺素；骨密度；大鼠，Sprague-Dawley；老年；种植体钛钉 中图分类号：R782 文献标志码：A DOI:10.11958/20190675
The effect and mechanism of parathyroid hormone on implant osseointegration in aged rats
People’s Hospital, Zhengzhou 450003, China; 2 Department of Stomatology, General Hospital of Pingmei Group Abstract: Objective To investigate the promoting effect of parathyroid hormone on implant osseointegration in aged rats and its mechanism. Methods Forty-eight aged rats (22-month-old) were divided into the elderly group and the parathyroid hormone group accorded to the random number method, with 24 rats in each group. Twenty-four adult rats (12month-old) were used as control group. Three groups of rats were implanted with titanium nails in the left tibia. Rats in the parathyroid hormone group were injected intraperitoneally with parathyroid hormone (20 µg/kg, qod), and rats in the control group and the elderly group were intraperitoneally injected with the same amount of normal saline for 8 weeks. The maxillary and tibia bone mineral densities were measured by dual-energy X-ray absorptiometry. Eight weeks after implantation, the bone-to-implant contact ration (BIC), trabercular area (TA), thickness of cortical bone (TCB), average trabecular width (TW) and combined bone width (CBLW) were measured. The alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) and interleukin-6 (IL-6) mRNA levels in bone tissues around the implant were detected by real-time quantitative polymerase chain reaction (qPCR). Results Before implantation, compared with the control group, the bone mineral densities of the maxilla and tibia were decreased in the elderly group and the parathyroid hormone group (P＜0.05). After 8week implantation, compared with the elderly group, the bone densities of maxillary and tibia in the parathyroid hormone group were increased (P＜0.05), the values of BIC, TA, TCB, TW and CBLW were increased (P＜0.05), the levels of ALP, OPN and OCN mRNA in the bone tissue around the implant were increased (P＜0.05), and the IL-6 mRNA level was decreased (P＜0.05). In the control group and parathyroid hormone group, the implant bone was well combined. In the elderly group, the implant bone was poorly coupled. Conclusion The bone density of aged rats is reduced, which affects the osseointegration of implants. Parathyroid hormone can increase bone mineral density in old rats and promote bone
1140
实验研究
Tianjin Med J, November 2019, Vol. 47 No. 11
甲状旁腺激素对老年大鼠种植体骨结合的 影响及机制研究
许东亮 1，彭朝晖2
摘要：目的 探讨甲状旁腺激素对老年大鼠种植体骨结合的促进作用及机制。方法 将 48 只 22 月龄老年大鼠 根据随机数字表法分为老年组和甲状旁腺激素组，每组 24 只，将 24 只 12 月龄成年大鼠作为对照组。3 组大鼠左侧胫 骨植入种植钛钉，甲状旁腺激素组大鼠腹腔注射甲状旁腺激素（20 µg/kg），隔日 1 次，对照组和老年组大鼠腹腔注射 等量生理盐水，共 8 周。采用双能 X 线骨密度测量仪测量上颌骨和胫骨骨密度。种植术后 8 周，测量种植体骨结合率 （BIC）、种植体周围松质骨区骨量（TA）、骨皮质厚度（TCB）、骨小梁平均宽度（TW）、结合骨板宽度（CBLW）。采用实 时定量聚合酶链反应（qPCR）种植体周围骨组织中碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OCN）、白细胞介素6（IL-6）mRNA 水平。结果 种植术前，与对照组比较，老年组和甲状旁腺激素组大鼠上颌骨和胫骨骨密度均降低 （P＜0.05）。种植术后 8 周，与老年组比较，甲状旁腺激素组大鼠上颌骨和胫骨骨密度升高（P＜0.05），BIC、TA、TCB、 TW、CBLW 值均升高（P＜0.05），种植体周围骨组织 ALP、OPN、OCN mRNA 水平升高（P＜0.05），IL-6 mRNA 水平降低 （P＜0.05）。对照组和甲状旁腺激素组大鼠种植体骨结合较好；老年组大鼠种植体骨结合差。结论 老年大鼠种植 体骨结合差，甲状旁腺激素可促进老年大鼠种植体骨结合。
XU Dong-liang1, PENG Zhao-hui2 1 Department of Stomatology, Henan Provincial People's Hospital, Zhengzhou University People’s Hospital, Henan University
The effect and mechanism of parathyroid hormone on implant osseointegration in aged rats
People’s Hospital, Zhengzhou 450003, China; 2 Department of Stomatology, General Hospital of Pingmei Group Abstract: Objective To investigate the promoting effect of parathyroid hormone on implant osseointegration in aged rats and its mechanism. Methods Forty-eight aged rats (22-month-old) were divided into the elderly group and the parathyroid hormone group accorded to the random number method, with 24 rats in each group. Twenty-four adult rats (12month-old) were used as control group. Three groups of rats were implanted with titanium nails in the left tibia. Rats in the parathyroid hormone group were injected intraperitoneally with parathyroid hormone (20 µg/kg, qod), and rats in the control group and the elderly group were intraperitoneally injected with the same amount of normal saline for 8 weeks. The maxillary and tibia bone mineral densities were measured by dual-energy X-ray absorptiometry. Eight weeks after implantation, the bone-to-implant contact ration (BIC), trabercular area (TA), thickness of cortical bone (TCB), average trabecular width (TW) and combined bone width (CBLW) were measured. The alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) and interleukin-6 (IL-6) mRNA levels in bone tissues around the implant were detected by real-time quantitative polymerase chain reaction (qPCR). Results Before implantation, compared with the control group, the bone mineral densities of the maxilla and tibia were decreased in the elderly group and the parathyroid hormone group (P＜0.05). After 8week implantation, compared with the elderly group, the bone densities of maxillary and tibia in the parathyroid hormone group were increased (P＜0.05), the values of BIC, TA, TCB, TW and CBLW were increased (P＜0.05), the levels of ALP, OPN and OCN mRNA in the bone tissue around the implant were increased (P＜0.05), and the IL-6 mRNA level was decreased (P＜0.05). In the control group and parathyroid hormone group, the implant bone was well combined. In the elderly group, the implant bone was poorly coupled. Conclusion The bone density of aged rats is reduced, which affects the osseointegration of implants. Parathyroid hormone can increase bone mineral density in old rats and promote bone
1140
实验研究
Tianjin Med J, November 2019, Vol. 47 No. 11
甲状旁腺激素对老年大鼠种植体骨结合的 影响及机制研究
许东亮 1，彭朝晖2
摘要：目的 探讨甲状旁腺激素对老年大鼠种植体骨结合的促进作用及机制。方法 将 48 只 22 月龄老年大鼠 根据随机数字表法分为老年组和甲状旁腺激素组，每组 24 只，将 24 只 12 月龄成年大鼠作为对照组。3 组大鼠左侧胫 骨植入种植钛钉，甲状旁腺激素组大鼠腹腔注射甲状旁腺激素（20 µg/kg），隔日 1 次，对照组和老年组大鼠腹腔注射 等量生理盐水，共 8 周。采用双能 X 线骨密度测量仪测量上颌骨和胫骨骨密度。种植术后 8 周，测量种植体骨结合率 （BIC）、种植体周围松质骨区骨量（TA）、骨皮质厚度（TCB）、骨小梁平均宽度（TW）、结合骨板宽度（CBLW）。采用实 时定量聚合酶链反应（qPCR）种植体周围骨组织中碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OCN）、白细胞介素6（IL-6）mRNA 水平。结果 种植术前，与对照组比较，老年组和甲状旁腺激素组大鼠上颌骨和胫骨骨密度均降低 （P＜0.05）。种植术后 8 周，与老年组比较，甲状旁腺激素组大鼠上颌骨和胫骨骨密度升高（P＜0.05），BIC、TA、TCB、 TW、CBLW 值均升高（P＜0.05），种植体周围骨组织 ALP、OPN、OCN mRNA 水平升高（P＜0.05），IL-6 mRNA 水平降低 （P＜0.05）。对照组和甲状旁腺激素组大鼠种植体骨结合较好；老年组大鼠种植体骨结合差。结论 老年大鼠种植 体骨结合差，甲状旁腺激素可促进老年大鼠种植体骨结合。
XU Dong-liang1, PENG Zhao-hui2 1 Department of Stomatology, Henan Provincial People's Hospital, Zhengzhou University People’s Hospital, Henan University