腹水脱落细胞学检查的操作流程

腹水脱落细胞学检查的操作流程英文回答：
Procedure for Cytological Examination of Ascitic Fluid.
1. Collection: Ascitic fluid should be collected using aseptic technique, preferably by paracentesis. The sample should be placed in a sterile container and sent to the cytology laboratory as soon as possible.
2. Preparation of Slides:
Direct smear: A drop of ascitic fluid is placed on a glass slide and air-dried.
Cytospin preparation: Ascitic fluid is centrifuged to concentrate the cells, which are then transferred to a slide using a cytospin centrifuge.
3. Staining: The slides are stained using a Romanowsky-
type stain, such as Wright-Giemsa or Papanicolaou stain.
4. Microscopic Examination: The stained slides are examined under a microscope by a trained cytologist. The cytologist evaluates the cellular composition, morphology, and any abnormal findings.
5. Interpretation: The cytological report typically includes a description of the cellular composition, any abnormal findings, and a diagnostic interpretation. The interpretation may include:
Benign: No malignant or premalignant cells are identified.
Malignant: Malignant cells are identified, indicating the presence of cancer.
Suspicious: Atypical or inconclusive cells are present, requiring further evaluation.
Non-diagnostic: The sample is inadequate or the
cellular composition is not interpretable.
中文回答：
腹水脱落细胞学检查操作流程。

1. 采集，腹水应使用无菌技术采集，最好通过腹腔穿刺术。

样品应置于无菌容器中，并尽快送至细胞学实验室。

2. 制片：
直接涂片，将一滴腹水滴在载玻片上，风干。

细胞旋进制备，对腹水进行离心以浓缩细胞，然后使用细胞旋进离心机将细胞转移到载玻片上。

3. 染色，使用罗曼诺夫斯基型染色剂（例如瑞氏-吉姆萨染色或巴氏染色）对载玻片进行染色。

4. 显微检查，由受过训练的细胞学家在显微镜下检查染色后的载玻片。

细胞学家评估细胞组成、形态和任何异常发现。

5. 解释：细胞学报告通常包括细胞组成描述、任何异常发现和诊断解释。

解释可能包括：
良性，未发现恶性或癌前细胞。

恶性，发现了恶性细胞，表明存在癌症。

可疑，存在非典型或不确定的细胞，需要进一步评估。

非诊断性，样本不足或细胞组织不可解释。