Carfilzomib_868540-17-4_DataSheet_MedChemExpress
EPZ015666_DataSheet_MedChemExpress
Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:EPZ015666 is an orally available inhibitor of PRMT5 enzymatic activity in biochemical assays with IC 50 of 22 nM and broad selectivity against a panel of other histone methyltransferases.IC50 & Target: IC50: 22 nM (PRMT5)[1]In Vitro: Treatment of MCL cell lines with EPZ015666 leads to inhibition of SmD3 methylation and cell death, with IC 50 values in the nanomolar range [1]. EPZ015666, a potent peptide–competitive and SAM–cooperative inhibitor with >10,000–fold specificity againstPRMT5 relative to other methyltransferases [2].In Vivo: EPZ015666 is orally bioavailable and amenable to in vivo studies. We performed 21–d efficacy studies in severe combined immunodeficiency (SCID) mice bearing subcutaneous Z–138 and Maver–1 xenografts, with twice–daily (BID) oral dosing on four dose groups: 25, 50, 100 and 200 mg per kilogram of body weight (mg/kg). After 21 d of continuous dosing, animals areeuthanized, and blood and tissues are analyzed to determine the relationship between methyl–mark pharmacodynamics andtumor–growth inhibition (TGI). EPZ015666 showed dose–dependent exposure and TGI after 21 d in both MCL models. Tumors in all EPZ015666 dose groups measured on day 21 showed statistically significant differences in weight, volume and tumor growth compared to vehicle–treated tumors. Dosing at 200 mg/kg BID induced tumor stasis in Z–138 cells, with >93% TGI after 21 d,whereas Maver–1 cells showed >70% TGI. Additionally, a third MCL xenograft is tested using the Granta–519 cell line, afast–growing model that reached endpoint on day 18 and showed dose–dependent efficacy with 45% TGI in the 200 mg/kg group.EPZ015666 is well tolerated in all three models, with minimal bodyweight loss in the 200 mg/kg dose group and no other clinical observations [1].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384–well polypropyleneV–bottom microplate. 3H–SAM is serially diluted twofold in assay buffer for a seven–point dilution series with a top concentration of 700 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 40 nM peptide (final assayconcentrations for both). Reactions are incubated for 60 min and quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration). For the peptide competition, EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384–well polypropylene V–bottom microplate. Peptide is serially diluted twofold in assay buffer for a seven–point dilution series with a top concentration of 480 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 75 nM 3H–SAM (final assay concentrations for both). Reactions are incubated for 60 min, and the reactions are quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration)[1].Cell Assay: EPZ015666 is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.2%)before use [1].[1]Cultured cells in linear/log–phase growth are split to a seeding density of 2×105 cells/mL in 2–20 mL of media,depending on the yield required at the end of the growth period. Compound is diluted in DMSO and added to each culture vesselProduct Name:EPZ015666Cat. No.:HY-12727CAS No.:1616391-65-1Molecular Formula:C 20H 25N 5O 3Molecular Weight:383.44Target:Histone Methyltransferase Pathway:Epigenetics Solubility:DMSOwith a final DMSO concentration of 0.2%. Cells are allowed to grow for 96 h undisturbed. At the conclusion of each treatment period, cells are harvested by centrifugation (5 min, 1,200 rpm), and cell pellets are rinsed once with PBS before being frozen on dry ice pending further processing. Long–term proliferation assays are performed on all MCL lines, with slight adjustments to initial seeding densities, depending on growth characteristics for each cell line. All assays are carried out for 12 d[1].Animal Administration: EPZ015666 is formulated in 20% N–N–dimethylacetamide in water (Mice)[1].[1]Mice[1]Male CD–1 mice (25–40 g; n=6, with 3 per time point) are treated with a single dose of EPZ015666 at 2 mg/kg by intravenoustail–vein injection and 10 mg/kg by oral gavage administration, with both doses formulated in 20% N–N–dimethylacetamide in water. Animals are fasted overnight and weighed before dose administration on the day of dosing. Approximately 30 μL ofblood are taken from animals by submandibular or retro–orbital bleeding at pre–specified time intervals (seven time points). For the last time point (24 h), samples are collected via cardiac puncture while the animals are under anesthesia (70% CO2:30% O2). Blood samples are transferred into K2–EDTA tubes and placed on wet ice before centrifugation at 4°C (3,000g, 15 min) to obtain plasma within 30 min after sample collection. Plasma samples are stored at -70±10°C before protein precipitation and LC–MS/MS analysis. We constructed standard calibration curves by analyzing a series of control plasma aliquots containing 100 ng/mL labetalol as an internal standard and 1–3,000 ng/mL EPZ015666. Four levels of quality control are also included in the analysis (3–2,400 ng/mL EPZ015666). Data are analyzed using Phoenix WinNonlin 6.2.1.References:[1]. Chan–Penebre E, et al. A selective inhibitor of PRMT5 with in vivo and in vitro potency in MCL models. Nat Chem Biol. 2015 Jun;11(6):432–7.[2]. Kryukov GV, et al. MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells. Science. 2016 Mar 11;351(6278):1214–8.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
Carfilzomib_868540-17-4_MSDS_MedChemExpress
Version 2.1 Revision Date: 07/08/2013Print Date: 11/25/2013MSDS1 Composition7 Accident Release MeasureProduct Name:CarfilzomibChemical Name:PROCEDURE(S) OF PERSONAL PRECAUTION(S)-Wear respirator, chemical safety goggles, rubber boots, and heavyrubber gloves.METHODS FOR CLEANING UP-Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area andwash spill site after material pickup is complete.L-Phenylalaninamide, (αS)-α-[[2-(4-morpholinyl)acetyl]amino]benzenebutanoyl-L-leucyl-N-[(1S)-3-methyl-1-[[(2R)-2-methyl-2-oxiranyl]carbonyl]butyl]-CAS No.:868540-17-48 Accident Release MeasureAppearance:white to off-white(solid)Formula:C40H57N5O79 Toxicological InformationSolubility:To the best of our knowledge, the chemical, physical, andtoxicological properties have not been thoroughly investigated.No data available.p p p p DMSO 50 mg/mL2 Handling and Storage10 Regulary Information3 Stability and Reactivity11Disposal ConsiderationsCLASSIFICATION- Substance not yet fully tested.SAFETY PHASES- 26-36 (In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.) 36/37/38 (Irritating to eyes,respiratory system and skin.)STABILITY- Stable under normal handling conditions.HANDLING- Do not breathe dust. Avoid contact with eyes,skin,and clothing.Avoid prolonged or repeated exposure.STORAGE- Please store the product under the recommended conditions in Certificate of Analysis.11 Disposal Considerations 4 Hazards Identification12 Transport Information5First Aid RID/ADR- Non-hazardous for road transport. IMDG- Non-hazardous for sea transport.IATA - Non-hazardous for air transport.As specific country, federal, state and local environmentalregulations vary and change frequently we suggest you contact a local, authorized waste disposal contractor for adequate disposal.Special indication of hazards to humans and the environment.Irritating to eyes, respiratory system and skin.MATERIALS TO AVOID- Strong oxidizing agents.REACTIVITY- May emit toxic gasses like Carbon monoxide,Carbon dioxide, Nitrogen oxides upon thermal decomposition.5 First Aid13 Other InformationThe above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Medchemexpress LLC shall not be held liable for any damage resulting from h dli f t t ith th b d tINHALATION- If inhaled, remove to fresh air. If not breathing give, artificial respiration. If breathing is difficult, give oxygen.SKIN CONTACT- In case of contact, immediately wash skin withsoap and copious amounts of water.EYE CONTACT- In case of contact, immediately flush eyes withcopious amounts of water for at least 15 minutes.INGESTION- If swallowed, wash out mouth with water provided person is conscious. Call a physician.6 Fire Fighting Measureshandling or from contact with the above product.EXTINGUISHING MEDIA Water spray- Carbon dioxide, dry chemical powder, or appropriate foam.SPECIAL RISKS Specific Hazard(s)- Emits toxic fumes under fire conditions. SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS Wear self-contained breathing apparatus and protective clothing Caution: Not fully tested. For research purposes onlyMedchemexpress LLCto prevent contact with skin and eyes.18 W i l k i n s o n W a y , P r i n c e t o n , N J 08540,U S AE m a i l : i n f o @m e d c h e m e x p r e s s .c o m W e b : w w w .m e d c h e m e x p r e s s .c o m。
家禽抗病毒新兽药复方金丝桃素颗粒的研究与应用
安全用药一、国内兽药市场和用药状况长期以来，由于疫病的困扰，抗生素、化学药物、激素类等药物滥用，造成家禽免疫力下降、生产性能下降，另外，由于抗生素、激素、化学药物的滥用，造成家禽交叉感染，耐药性上升，使疾病千变万化，病因也日益复杂，导致家禽发生疾病时必须增大使用剂量或反复多次使用多种药物，甚至延长治疗时间，然而随着大复方药品进入兽药市场，使疾病病因多种多样随后产生不同病因的并发症状，致使药物残留日益严重，不可否认我国动物疾病和药物残留一直存在，药残问题已严重威胁食品安全和国民的身体健康。
此外，抗生素耐药性的危害不仅表现在药物剂量增大、疗程延长、复发率升高等多方面，还表现为引起并发症，使抗生素失去疗效，导致家禽死亡率升高。
这一问题已引起了社会各界的广泛重视，因此向市场提供“绿色安全食品”，严格控制食品中抗生素、激素等药物残留已势在必行。
中国是一个中药大国，具有两千多年来的历史，如何更好的使用中药材，发挥中国中药特色，更好的为禽类养殖者服务，保证人类健康，始终是国家和全民关注的热点话题。
二、病毒性疾病在家禽方面的危害冬春两季是家禽病毒性疾病的高发季节和诱发时段。
近年来，冬季阶段北方各省家禽非典型性流感、强毒性新城疫、病毒性支气管炎等一些病毒病普遍存在，发病率高、流行迅速、混感和继发性感染尤为严重，尤其是蛋鸡表现较为明显，肉鸡方面出现发病率高，死亡率高，给养殖户带来了很大的经济损失和比较头痛的难题。
三、复方抗病毒中兽药中主要成分与药理作用用于禽类抗病毒的中药有黄芪多糖、连翘、板蓝根等，此外还有金丝桃素、紫雏菊提取物等，禽类病毒性疾病主要靠提高机体免疫力或使用抗病毒中药效果更佳，不能滥用国家禁用药品比如金刚烷胺、利巴韦林、金刚乙胺等，在提高家禽机体免疫力方面笔者推荐使用人参茎叶总皂苷，与此同时由于病毒感染、混感或者继发感染家禽发病，症状为高热不退，需要使用强心、镇静、抗炎、抗感染、清热解毒和凉血的中药比如水牛角浓缩粉，一方面杀灭病毒控制病灶病毒复制，另一方清热解毒凉血，从兽医兽药理论上讲标本兼治，而不能一味的屡次大量使用副作用较大的抗生素、激素和安乃近、氨基比林之类的解热镇痛药。
卡非佐米中文说明书
【药物名】Carfilzomib【商品名】Kyprolis【通用名】卡非佐米【美国初次批准】2012年7月20日多发性骨髓瘤【类别】小分子【靶点】蛋白酶体抑制剂【分子结构】分子式：C40H57N5O7分子量：719.9【生产公司】Onyx Pharmaceuticals，Inc 奥尼克斯制药公司【剂型和规格】单次使用小瓶：60 mg无菌冻干粉【本质】注射用KYPROLIS(Carfilzomib)是一种抗肿瘤药物只供利用静脉使用。
KYPROLIS是一种无菌，白色至灰白色冻干粉和可得到单次使用小瓶。
每小瓶KYPROLIS含60 mg的Carfilzomib，3000 mg 磺丁基醚β-环糊精，57.7 mg 枸橼酸，和氢氧化钠为调整pH(目标pH 3.5)。
Carfilzomib是一种修饰的四肽基环氧化物，分离为游离碱结晶。
Carfilzomib是实际上不溶于水，和在酸性条件非常轻微溶解。
【作用机理】Carfilzomib是一种四肽基环氧骨架蛋白酶体抑制剂不可逆地结合至20S蛋白酶体含苏氨酸N-端活性部位，26S蛋白酶体内蛋白水解核心颗粒。
Carfilzomib有抗增殖和凋亡活性在体外在实体和血液学中粒细胞。
在动物中，Carfilzomib抑制蛋白酶体活性在血液和组织和在多发性骨髓瘤，血液学，和实体瘤模型中延迟肿瘤生长。
【适应症和用途】KYPROLIS是适用为多发性骨髓瘤患者的治疗，患者曾接收至少两种既往治疗包括硼替佐米和一种免疫调节药和已证实疾病进展或末次治疗的完成60天内。
批准是根据反应率。
尚未证明临床获益，例如活存或症状改善。
【用法用量】KYPROLIS静脉历时2至10分钟给药，在两连续天，每周共3周(第1，2，8，9，15,和16天)，接着是12天休息期(第17至28天)。
被考虑一个治疗疗程各28天期(表1)。
在疗程1中，KYPROLIS被给予在剂量20 毫克/平方米。
如在疗程1中耐受，在疗程2开始剂量应被递增至27 毫克/平方米和在随后疗程中继续27 毫克/平方米。
抗原恢复缓冲液（100X Tris-EDTA缓冲液，pH 9.0）说明书
Product nameAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)Tested applicationsSuitable for: IHC-P General notes Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed,paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies thatrequire Tris-EDTA buffer (pH 9.0) pretreatment.This product contains detergent for emulsification of the paraffin.1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.Protocol:Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plasticwrap, place in microwave and set high power to boil and then set low power to keep itboiling for 10 min. Let the sections cool in the microwave for at least 20min.Wash sections with hot tap water for 1 minute.Wash sections in buffer for 2x3 minutes.Continue with IHC protocolOther kits and reagents for IHCOther antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes .Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization,counterstaining, and mounting in the IHC kits and reagents guide .FormLiquid Storage instructionsStore at room temperature.Storage buffer pH: 8.7Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA, 5% TrisBuffer 100x concentrated with detergent.Product datasheetAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)ab936843 Abreviews 22 References 3 ImagesOverview PropertiesThe Abpromise guaranteeImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Image from M aniati et al., Cell Rep.;30(2):525-540.e7; doi: 10.1016/j.celrep.2019.12.034. Reproduced under the Creative Commons licensehttps:///licenses/by/4.0/Immunohistochemical for RUNX2 on murine omental metastasis Antigen retrieval: Tris-EDTA, pH;9 (ab93684), Blocking: Normal Goat Serum, Primary: Rabbit monoclonal anti-Runx2 [EPR14334] (ab192256), dilution 1:1000, Secondary: HRP anti-rabbitImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)ab93684 Antigen Retrieval Buffer 100X Tris-EDTA Buffer, pH 9ApplicationsOur Abpromise guarantee covers the use of ab93684 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews NotesIHC-P Use at an assay dependent dilution.ImagesImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).Please note: A ll products are "FOR RESEA RCH USE ONLY. NOT FOR USE IN DIA GNOSTIC PROCEDURES"Our Abpromise to you: Quality guaranteed and expert technical supportReplacement or refund for products not performing as stated on the datasheetValid for 12 months from date of deliveryResponse to your inquiry within 24 hoursWe provide support in Chinese, English, French, German, Japanese and SpanishExtensive multi-media technical resources to help youWe investigate all quality concerns to ensure our products perform to the highest standardsIf the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https:///abpromise or contact our technical team.Terms and conditionsGuarantee only valid for products bought direct from Abcam or one of our authorized distributors。
迪雷尔（Drexel）乙醛三氯尿唑（Chlorpyrifos）15G农业昆虫毒（Agricultur
SAFETY DATA SHEETDREXEL CHLORPYRIFOS 15G AGRICULTURAL INSECTICIDEProduct Name: Drexel Chlorpyrifos 15G Agricultural InsecticideEPA Reg No.: 19713-505CAS NO: 2921-88-2Formula: C9H11ClNO3PSCompany: Drexel Chemical Company1700 Channel AvenueMemphis, TN 38106Synonyms: O,O-Diethyl O-3, 5, 6-trichloropyridin-2-yl phosphorothioateIdentifiers:EINECS: 220-864-4RTECS: TF6300000DOT information: See Section 14 for Transportation InformationEmergency Telephone Number:CHEMTREC Drexel Chemical Co.Tel: 1-800-424-9300 901-774-4370This product is an EPA FIFRA registered pesticide. Some of the classifications on this SDS are not the same as the FIFRA label. Certain sections of this SDS are superseded by federal law governed by EPA for a registered pesticide. Please see Section 15. REGULATORY INFORMATION for explanation.GHS classification:Health hazards: Acute toxicity – inhalation Category 2Skin corrosion/irritation Category 2Eye damage/ irritation Category 2BSpecific target organ toxicity –(single exposure) Category 1Specific target organ toxicity –(repeated exposure) Category 2Aquatic acute toxicity Category 1GHS label elements:Signal Word: DANGERHazard Statements: Toxic if swallowed.Fatal if inhaled.Causes skin irritation.Causes eye irritation.Causes damage to nervous system.May cause damage to nervous system through prolonged or repeated exposure.Very toxic to aquatic life.Precautionary Statements:Prevention: Prevention Wash thoroughly after handling.Do not breathe dust/fume/gas/mist/vapors/spray.Wear protective gloves/protective clothing. Examples of preferred glove barriermaterials include: Neoprene, Nitrile/butadiene rubber (“nitrile” or “NBR”) orPolyvinyl chloride (“PVC” or “vinyl”).Wear respiratory protection. Use a respirator with either an organic vapor-removing cartridge with a pre-filter approved for pesticides (MSHA/NIOSHapproval number prefix TC-23C) or a canister approved for pesticides(MSHA/NIOSH approval number prefix TC-14G).Do not eat, drink or smoke when using this product.Use only outdoors or in well-ventilated area.In case if inadequate ventilation wear respiratory protection.Avoid release to the environment from other than intended use.Response: If swallowed: Immediately call a poison control center/doctor. Rinse mouth. SeeSection 4: Note to Physician.If on skin: Wash with plenty of water. Call a poison center/doctor if you feelunwell. If skin irritation occurs; Get medical advice/attention. Take offcontaminated clothing and wash it before reuse.If inhaled: Remove person to fresh air and keep comfortable for breathing.Immediately call a poison center/doctor if person stops breathing.If in eyes: Rinse cautiously with water for several minutes. Remove contactlenses, if easy to do. Continue rinsing. If eye irritation persists: Get medicaladvice/attention.If exposed: Call poison center/doctor. Specific treatment see Note toPhysician, Section 4.Get medical attention if you feel unwell.Collect spillage.Storage: Store locked up. Store in well-ventilated place.Keep cool.Keep container tightly closed.Disposal: Disposal of contents/container must be in accordance with your local or arearegulations.Components CAS No.: % By Wt.: OSHA PEL: ACGIH TLV:Active Ingredient:Chlorpyrifos 2921-88-2 15.0% N/Av 0.2 mg/m3Inert Ingredients: N/A 85.0% N/A N/AHave the product container or label with you when calling a poison control center or doctor.Eye Contact: Hold eye open and rinse slowly and gently with water for 15 to 20 minutes. Remove contact lenses, if present, after the first 5 minutes, then continue rinsing eyes for at least 10 minutes. Obtain medical attention without delay, preferably from an ophthalmologist.If Swallowed: Call a poison control center or doctor immediately for treatment advice. Rinse out mouth then have person sip a glass of water if able to swallow. Do not induce vomiting unless told to do so by the poison control center or doctor. Do not give anything by mouth to an unconscious person. Have product label with you when calling a poison control center or doctor.Skin Contact: Immediately flush skin with water while removing contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Destroy contaminated leather items such as shoes, belts, and watchbands.If Inhaled: Move person to fresh air. If person is not breathing, call 911 or an ambulance, then give artificial respiration, preferably mouth-to-mouth, if possible. Call a poison control center or doctor for further treatment advice.Note to Physician: This product contains an organophosphate that inhibits cholinesterase. Treat symptomatically. If exposed, plasma and red blood cell cholinesterase tests may indicate significance of exposure (baseline data are useful). Atropine, only by injection, is the preferable antidote. Oximes, such as 2-PAM/protopam, may be therapeutic if used early; however, use only in conjunction with atropine. In case of severe, acute poisoning, use antidote immediately after establishing an open airway and respiration. Contains petroleum distillate. Do not induce vomiting since vomiting may cause aspiration pneumonia.Fire Hazards: Thermal decomposition during a fire can produce fumes and irritating gases.Flammability classification (OSHA 29 CFR 1910.1200): N/AFlash point: NoneLower flammable limit (% by volume): N/AvUpper flammable limit (% by volume): N/AvFire Fighting Procedures: Keep people away. Isolate fire and deny unnecessary entry. Evacuate the area and fight the fire from upwind at a safe distance to avoid hazardous vapors or decomposition products. Dike and collect fire-extinguishing water to prevent environmental damage and excessive waste runoff.Firefighting media: Use foam, dry chemical, carbon dioxide, or water fog when fighting fires involving this product. Do not use water jet, as this may spread burning material. Minimize the use of water to avoid environmental contamination. Contain all runoff.Special Protective Equipment for Firefighters: Wear positive-pressure self-contained breathing apparatus (SCBA) and protective firefighting clothing (includes firefighting helmet, coat, trousers, boots, and gloves). Use full face shield and operate in positive pressure mode. Avoid contact with this material during firefighting operations. If contact is likely, change to full chemical resistant firefighting clothing with self-contained breathing apparatus. If this is not available, wear full chemical resistant clothing with self-contained breathing apparatus and fight fire from a remote location. For protective equipment in post-fire or non-fire clean-up situations, refer to the relevant sections.Hazardous Combustion Products: Hydrogen chloride, ethyl sulfide, diethyl sulfide, nitrogen oxides, carbon oxides, irritating fumes and smoke.NFPA: Health: Flammability: Reactivity:2 1 0(Rating: 4-Extreme, 3-High, 2-Moderate, 1-Slight, 0-Insignificant)Steps to be taken if Material is Released or Spilled:Sweep as much material as possible, keeping dust to a minimum and place in an approved chemical waste container. Wash the spill area with water containing a strong detergent, absorb with earth, sand or absorbent material and sweep up and place in approved chemical waste container. For large spills contact Drexel Chemical Co. See Section 13, Disposal Considerations, for additional information.Personal Precautions:Isolate area. Keep unnecessary and unprotected personnel from entering the area. Refer to Section 7, Handling for additional precautionary measures. Ventilate area of leak or spill. Use appropriate safety equipment. For additional information, refer to Section 8, Exposure Controls and Personal Protection.Environmental Precautions: Prevent from entering into soil, ditches, sewers, waterways and/or groundwater. See Section 12, Ecological Information.KEEP OUT OF REACH OF CHILDRENGeneral Handling: Avoid contact with eyes, skin, and clothing. Wash thoroughly after handling. Do not swallow. Avoid breathing dust. Use with adequate ventilation. Wear chemical protective equipment when handling. Keep away from heat, sparks and flame. See Section 8, Exposure Controls and Personal Protection.Storage: Store in a cool, dry, ventilated and secure area designated specifically for pesticides and away from heat sources. Keep in original containers and keep containers closed when not in use. Do not store in excessive heat. Do not store near children, food, foodstuffs, drugs or potable water supplies.Exposure Limits: TLV Chlorpyrifos 0.2 mg/m3Personal Protection:Eye/Face Protection: Wear safety glasses with side shields or chemical splash goggles to prevent dust from entering the eyes. If using a full face shield, always use safety glasses or goggles along with the face shield to ensure adequate protection of the eyes.Skin Protection: Wear long-sleeved shirt, long pants and shoes plus socks. Safety shower should be located in immediate work area. Remove contaminated clothing immediately after handling this product, wash skin area with soap and water, and launder clothing before reuse or dispose of properly. Items which cannot be decontaminated, such as shoes, belts and watchbands, should be removed and disposed of properly.Hand protection: Use gloves chemically resistant to this material. Examples of preferred glove barrier materials include: Neoprene, Nitrile/butadiene rubber (“nitrile” or “NBR”) or Polyvinyl chloride (“PVC” or “vinyl”).Respiratory Protection: Not normally required. Respiratory protection should be worn when there is a potential to exceed the exposure limit requirements or guidelines. When handling in enclosed areas, when large quantities of dusts are generated or prolonged exposure is possible in excess of the TLV, use a respirator with either an organic vapor-removing cartridge with a prefilter approved for pesticides (MSHA/NIOSH approval number prefix TC-23C) or a canister approved for pesticides (MSHA/NIOSH approval number prefix TC-14G).Ingestion: Avoid ingestion of even very small amounts; do not consume or store food or tobacco in the work area; wash hands and face before smoking or eating.Engineering Controls:Ventilation: When handling this product proper ventilation is required to maintain exposure below the TLV.Ventilate all transport vehicles prior to unloading. Facilities storing or utilizing this material should be equipped with and eyewash facility and safety shower.Physical State: GranuleColor: BrownOdor: MildFlash Point: NoneVapor Pressure (mmHg): N/ABoiling Point: N/AVapor Density (air = 1): N/ABulk Density (H2O = 1): 46.43 lbs./ft3Freezing Point: N/ASolubility in water: InsolublepH: N/AViscosity: N/A% Volatiles: >3%Stability/Instability: Avoid heating above 60°C (100°F). Chlorpyrifos undergoes exothermic decomposition at approximately 130°C (266°F), which can lead to higher temperatures and violent decomposition if generated heat is not removed.Conditions to Avoid: Keep this product away from excessive heat and moisture.Incompatible Materials: Avoid contact with strong alkalies, amines and oxidizers.Hazardous Polymerization: Will not occurThermal Decomposition: Decomposition products can include but are not limited to: Hydrogen chloride, ethyl sulfide and nitrogen oxides.Data presented for technical Chlorpyrifos:Acute ToxicityIngestion:∙Oral LD50, (rat): 223 mg/kgDermal:∙Dermal LD50, (rabbit): >5,000 mg/kgInhalation:∙LC50, (rat): > 0.2 mg/lEye Irritation (rabbit):∙Slight irritation resolved within 24 hoursSkin Irritation (rabbit):∙Mild irritation resolved within 7 daysSensitization Skin:∙Non-sensitizer (Guinea Pig)Carcinogenicity:∙Not likely to be carcinogenic in humansTeratogenicity, mutagenicity, and other reproductive effects: None knownData presented for the active ingredient Chlorpyrifos:ENVIRONMENTAL FATE:∙Very highly toxic to fish, highly toxic to daphnia, moderately to highly toxic to birds and serious hazard to Honeybees.Persistence and Degradability:∙Moderately persistent in soils degrading slowly through aerobic and anaerobic metabolism. Absorbs strongly to soil particles and is not readily soluble in water.Aquatic Toxicity:∙Rainbow Trout: 96 hour LC50: (0.007 – 0.051 mg/L)∙Bluegill Sunfish: 96 hour LC50: (0.002 – 0.010 mg/L)∙Daphnia magna: 48 hour EC50: (1.7 μg/L)Bees:∙LD50: (oral) 360 ng/bee; (contact) 70 ng/beeBird Toxicity:∙Mallard Duck: 8-day LC50: 180 ppm∙Bobwhite Quail: 8-day LC50: 423 ppmIf wastes and/or containers cannot be disposed of according to the product label directions, disposal of this material must be in accordance with your local or area regulatory authorities. This information presented below only applies to the material as supplied. The identification based on characteristic(s) or listing may not apply if the material has been used or otherwise contaminated. It is the responsibility of the waste generator to determine the toxicity and physical properties of the material generated to determine the proper waste identification and disposal methods in compliance with applicable regulations. If the material as supplied becomes a waste, follow all applicable regional, national and local laws.DOT: UN-3077, Environmentally hazardous substances, solid, n.o.s., (Chlorpyrifos), 9, PG III, Marine Pollutant, RQ 1 lb.IMDG: UN-3077, Environmentally hazardous substances, solid, n.o.s., (Chlorpyrifos), 9, PG III, Marine Pollutant, RQ 1 lb.IATA: UN-3077, Environmentally hazardous substances, solid, n.o.s., (Chlorpyrifos), 9, PG III, Marine Pollutant, RQ 1 lb.Freight description: Agricultural Insecticide, solid, n.o.s.ERG Guide No.: 171This information is not intended to convey all specific regulatory or operational requirements/information relating to this product. Additional transportation system information can be obtained through an authorized sales or customer service representative. It is the responsibility of the transporting organization to follow all applicable laws, regulations and rules relating to the transportation of the material.OSHA Hazard Communication Standard:∙This product is a “Hazardous Chemical” as defined by the OSHA Hazard Communication Standard, 29 CFR 1910.1200.∙EPA FIFRA INFORMATION:This chemical is a pesticide product registered by the United States Environmental Protection Agency and is subject to certain labeling requirements under federal pesticide law. These requirements differ from the classification criteria and hazard information required for safety data sheets (SDS), and for workplace labels of non-pesticide chemical. The hazard information required on the pesticide label is listed out below.The pesticide label also includes other important information, including directions for use.∙EPA/CERCLA Reportable Quantity: Chlorpyrifos RQ = 1 lb.SARA/TITLE III:∙Section 302. Extremely Hazardous Substance Notification: This material is not known to contain any Extremely Hazardous Substances.∙Section 311/312. Hazard Categories:Fire HazardImmediate health hazardChronic health hazard∙Section 313. Toxic Chemical(s): This material is not known to contain any Toxic Chemical constituents.∙RCRA Waste Code: Not applicableCalifornia Proposition 65 (Safe Drinking Water and Toxic Enforcement Act of 1986):∙This product is not listed.Toxic Substances Control Act (TSCA):∙All components of this product are on the TSCA Inventory or are exempt from TSCA Inventory requirements under40 CFR 720.30Drexel Chemical Company recommends that each customer or recipient of this SDS to study it carefully and consult appropriate expertise, as necessary or appropriate, to become aware of and understand the data contained in this SDS and any hazards associated with the product. The information herein is provided in good faith and believed to be accurate as of the effective date shown below. However, no warranty, express or implied, is given. Regulatory requirements are subject to change and may differ between various locations. It is the buyer’s/user’s responsibility to ensure that his activities comply with all federal, state, provincial or local laws. The information presented here pertains only to the product as shipped. Since conditions for use of the product are not under the control of the manufacturer, it is the buyer’s/user’s duty to determine the conditions necessary for the safe use of this product. Due to the proliferation of sources for information such as manufacturer-specific SDSs, we are not and cannot be responsible for SDSs obtained from any source other than ourselves. If you have obtained an SDS from another source or if you are not sure that the SDS you have is current, please contact us for the most current version.Date Revised: June 22, 2016Supersedes: September 8, 2015。
Tumor cells from human, mouse, and xenografted tumors - EACR 2016
Novel methods for the isolation of tumor cells from human, mouse, and xenografted tumorsDavid Agorku¹, Anne Langhammer¹, Lena Willnow¹, Kerstin Klingner², Stefan Tomiuk¹, Jutta Kollet¹, Silvia Rüberg¹, Julia Schueler², Andreas Bosio¹, and Olaf Hardt¹1 Miltenyi Biotec GmbH, Bergisch Gladbach, Germany, 2 Oncotest GmbH, Freiburg, GermanyIntroductionSolid tumors are vascularized and infiltrated by stromal cells such as leukocytes, endothelial cells, and fibroblasts¹. The amount and composition of those non-tumor cells depends on various factors including tumor entity and stage, treatment history, status of the host organism and site of tumor growth. The widely unpredictable and variable amount of non-tumor cells makes analyses of tumor samples difficult. Contaminating cells lead to hybridization of non-tumor cell–derived mRNA molecules to probes on microarrays, and a significant reduction of sensitivity caused by measurement of irrelevant signals during next-generation sequencing or proteome analysis can be expected. In addition, the culture of tumor cells is frequently hampered by fibroblasts overgrowing the target cells, which biases assays such as drug sensitivity tests.To overcome these limitations, we have developed fast and easy methods to isolate ‘untouched’ tumor cells from tissue samples. The underlying procedure is based on thecomprehensive depletion of cells of non-tumor origin by combining automated tissue dissociation and magnetic cell sorting. A negative selection strategy enables the isolation of the tumor cell population without specific knowledge of surface marker expression on these cells. Even from samples initially containing low numbers of tumor cells (<20%), the target cells could be isolated to purities of higher than 95% in less than 20 minutes. Here, we have applied these methods to isolate tumor cells from primary human breast carcinoma, three different syngeneic mouse tumor models, and three different patient-derived xenograft models. Bulk tumor and isolated tumor cells were cultivated for up to seven days. Additionally, we performed whole exome sequencing (WES) of bulk human tumor xenografts from lung, bladder, and kidney cancer, and compared the results to samples depleted of mouse cells.Conclusion• Three novel methods have been established allowing for the untouched isolation of tumor cells from mouse, human, and xenotransplanted tumor tissue.• The cell separation methods are easy and fast (<20 min) and allow for accurate downstream analysis of tumor cells, avoiding bias caused by contaminating cells of the tumor microenvironment.• The contaminating non-tumor cells are specifically labeled prior to their depletion. Labeling of the tumor cells is not required. Therefore, the procedures can be used for the isolation of most tumor types without the need for knowledge of a positive marker expressed on the target cells.• Isolation of pure populations of tumor cells improves downstream culture and molecular analysis by NGS.ResultsRapid isolation of untouched tumor cells1C D 326 (E p C A M )-V i o B l u e ®10¹10² C D 45-P E -V i o ® 7700010²10¹Forward scatter S i d e s c a t t e r Anti-Fibroblast-FITC GlyA-APC C D 45-P E -V i o 770CD31-PEC D 326 (E p C A M )-V i o B l u e We have performed screenings on primary tumor material, cell lines, and healthy tissues to define combinations of antibodies recognizing all cells of the tumor microenvironment but not the tumor cells. Conjugates of these antibodies with superparamagnetic nanoparticles were used to develop optimized procedures for the depletion of non-tumor cells from mouse, human, and xenotransplanted tumor samples by magnetic separation (fig. 1A). The procedure allows for the elimination of >95% of the contaminating cells in less than 20 min, as shown for the isolation of tumor cells from a primary human tumor sample (fig. 1B). To evaluate the depletion efficiency by flow cytometry, cell fractions were labeled with human lineage markers (CD31,CD45, Gly-A, and anti-Fibroblast) and an antibody against human CD326 (EpCAM).Appropriately adapted antibody combinations allowed for the analysis of xenografted or syngeneic mouse tumors (fig. 1 C and D). As the antibody cocktails were developed to deplete the unwanted non-tumor cells, the isolation is independent of tumor cell–specific surface markers. Therefore, tumor cells can be isolated regardlessof the tumor entity, as shown for the isolation of tumor cells from different mouse tumors, which were induced by GFP-expressing cell lines (fig. 1C), and different entities of human tumor xenografts (fig. 1D). Additionally, the isolated cells stayed ‘untouched’ allowing for subsequent sorting of tumor subpopulations by MACS® Technology.10³-10110¹10²010³10²10¹-1110³-10110¹10²010³10²10¹-1110³-10110¹10²010³10²10¹-1110³-10110¹10²010³10²10¹-11Cultivation of tumor cells from primary specimens is frequently hampered by the presence of fibroblasts, red blood cells, and debris. While debris and red blood cells impair efficient plating of tumor cells, fibroblasts attach and expand more efficiently, thereby overgrowing the target cells. Even when the target cells attach and grow well, in vitro cell culture assays (e.g. drug cytotoxicity testing) are problematic since mathematical correction for effects originating from contaminating cells is impossible in most cases. Upon magnetic separation, the original bulk andisolated tumor cellfractions were cultured for three to seven days, fixed, and stained. Syngeneic mousetumor cells were detected by tumor cell–specific GFP expression and fibroblasts were stained with alpha-smooth muscle actin (α-SMA) (fig. 2, middle). Human tumors were stained for the human-specific epithelial tumor marker CD326 (EpCAM). As the human tumor cells were negative for vimentin, we were able to use this marker to unambiguously identify fibroblasts (fig. 2, top and bottom). Even after seven days, the cultures of isolated tumor cells were nearly pure.Depletion of non-tumor cells improves downstream culture of target cells2V i m e n t i n / E p C A M / D A P IH u m a n t u m o r Bulk tumor cellsIsolated tumor cellsS y n g e n e i c m o u s e t u m o r α-S M A / e G F P / D A P IX e n o g r a f t t u m o r V i m e n t i n / E p C A M / D A P IFigure 2References1. DeRose, Y.S. et al. (2011) Nat. Med. 17: 1514–1520.2. Bolger, A.M. et al. (2014) Bioinformatics 30: 2114–2120.3. Li, H. and Durbin, R. (2009) Bioinformatics 25: 1754–1760.Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec GmbH. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2016 Miltenyi Biotec GmbH. All rights reserved.Samples of human tumor xenografts contain a significant amount of host-derived cells. To assess the impact of depletion of non-tumor cells on the quality of next-generation sequencing data, we conducted WES on three different xenograft models derived from human kidney, lung, and bladder cancer subsequent to mouse cell depletion. DNA from bulk tumor or isolated tumor cells was used to produce exome-captured sequencing libraries applying the Nextera® Rapid Capture Exome K it (Illumina®). For sequencing on a MiSeq® instrument (Illumina) the MiSeq Reagent K it v3 (150 cycles, Illumina) was utilized to generate 75-bp paired-end reads. As the capture oligonucleotides used for targeted enrichment of protein-coding sequences were designed based on the human genome, an initial pre-enrichment of DNA fragments of human origin from the mixture of mouse and human cells was expected. In order to assess the number of capture oligonucleotides that might cross-hybridize with mouse genomic DNA, we conducted BLAST searches of each single Nextera probe against mouse genome and used the resulting alignment parameters to determine possible cross-hybridization. Depending on the selection thresholds (alignment length, no. of mismatches, no. of gaps), we predicted a cross-reactivity of 5–10% of captureprobes with mouse transcripts (data not shown). A significant increase (p < 0.05) in clusterdensity (not shown) as well as an average increase in read counts of 33% was observed for the samples depleted of mouse cells, indicating improved sample quality (fig. 3A). Correspondingly, we observed a strong reduction of debris and dead cells upon mouse cell depletion by flow cytometry analysis (data not shown).After adapter clipping (trimmomatic v0.32²), we mapped the reads of all samples against human and mouse genomes (bwa v0.7.12³) and determined their putative origin based on the respective alignment parameters (LINUX shell, command-line Perl) (fig. 3B). An average of 12% of reads derived from bulk tumor samples was attributed to mouse cells. This amount could be reduced to 0.3% by prior depletion of mouse cells (fig. 3C). As on average 15% of the mouse-derived reads mapped erroneously to the human genome (1.9% of total reads) in the bulk tumor samples, a strong positive influence of mouse cell depletion (0.04% of total reads erroneously mapped to human genome) on downstream analyses can be expected. Figure 3C exemplifies the detailed read assignment for bulk tumor and isolated human tumor cells derived from the bladder cancer xenograft.Improved downstream analysis upon isolation of target cells3。
安捷伦产品目录
15
Real-Time PCR
16
Mx3000P QPCR System
17
Brilliant III Ultra-Fast SYBR Green QPCR and QRT-PCR Reagents
18
Brilliant III Ultra-Fast QPCR and QRT-PCR Reagents
Agilent / STRATAGENE
Agilent website: /genomics
Welgene | Agilent Stratagene
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Table of Content
Table of Contents
/ XL1-Red Competent Cells SoloPack Gold Supercompetent Cells
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適用於 UNG 去汙染或 bisulphite
sequencing
適用於 TA Cloning
最高敏感性
取代傳統 Taq 的好選擇
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2
威健股份有限公司 | Stratagene 總代理
PCR Enzyme & Instrument
Agilent SureCycler 8800
市場上領先的 cycling 速度和 sample 體積 10 ～ 100 μL 簡易快速可以選擇 96 well 和 384 well 操作盤 優秀的溫控設備讓各個 well 都能保持溫度的穩定 七吋的高解析度觸控螢幕讓操作上更為簡便 可以透過網路遠端操控儀器及監控儀器 Agilent 專業的技術支援可以幫助您應對各種 PCR 的問題
食品添加剂欧盟编码一览表
•E100 Curcumin•E101 (i) Riboflavin (ii) Riboflavin-5'-phosphate•E102 Tartrazine•E104 Quinoline Yellow•E110 Sunset Yellow FCF, Orange Yellow S•E120 Cochineal, Carminic acid, Carmines•E122 Azorubine, Carmoisine•E123 Amaranth•E124 Ponceau 4R, Cochineal Red A•E127 Erythrosine•E128 Red 2G•E129 Allura Red AC•E131 Patent Blue V•E132 Indigotine, Indigo carmine•E133 Brilliant Blue FCF•E140 Chlorophylis and Chlorophyllins: (i) Chlorophylls (ii) Chlorophyllins•E141 Copper complexes of chlorophylls and chlorophyllins (i) Copper complexes of chlorophylls (ii) Copper complexes of chlorophyllins •E142 Greens S•E150a Plain caramel•E150b Caustic sulphite caramel•E150c Ammonia caramel•E150d Sulphite ammonia caramel•E151 Brilliant Black BN, Black PN•E153 Vegetable carbon•E154 Brown FK•E155 Brown HT•E160a Carotenes: (i) Mixed carotenes (ii) Beta-carotene•E160b Annatto, bixin, norbixin•E160c Paprika extract, capsanthin, capsorubin•E160d Lycopene•E160e Beta-apo-8'-carotenal (C 30)•E160f Ethyl ester of beta-apo-8'-carotenic acid (C 30)•E161b Lutein•E161g Canthaxanthin•E162 Beetroot Red, betanin•E163 Anthocyanins•E170 Calcium carbonates•E171 Titanium dioxide•E172 Iron oxides and hydroxides•E173 Aluminium•E174 Silver•E175 Gold•E180 Latolrubine BK•E200 Sorbic acid•E202 Potassium sorbate•E203 Calcium sorbate•E210 Benzoic acid•E211 Sodium benzoate•E212 Potassium benzoate•E213 Calcium benzoate•E214 Ethyl p-hydroxybenzoate•E215 Sodium ethyl p-hydroxybenzoate•E216 Propyl p-hydroxybenzoate•E217 Sodium propyl p-hydroxybenzoate•E218 Methyl p-hydroxybenzoate•E219 Sodium methyl p-hydroxybenzoate•E220 Sulphur dioxide•E221 Sodium sulphite•E222 Sodium hydrogen sulphite•E223 Sodium metabisulphite•E224 Potassium metabisulphite•E226 Calcium sulphite•E227 Calcium hydrogen sulphite•E228 Potassium hydrogen sulphite•E230 Biphenyl, diphenyl•E231 Orthophenyl phenol•E232 Sodium orthophenyl phenol•[E233 Thiabendazole] - Item deleted by Directive 98/72/EC•E234 Nisin•E235 Natamycin•E239 Hexamethylene tetramine•E242 Dimethyl dicarbonate•E249 Potassium nitrite•E250 Sodium nitrite•E251 Sodium nitrate•E252 Potassium nitrate•E260 Acetic acid•E261 Potassium acetate•E262 Sodium acetates (i) Sodium acetate (ii) Sodium hydrogen acetate (sodium diacetate)•E263 Calcium acetate•E270 Lactic acid•E280 Propionic acid•E281 Sodium propionate•E282 Calcium propionate•E283 Potassium propionate•E284 Boric acid•E285 Sodium tetraborate (borax)•E290 Carbon dioxide•E296 Malic acid•E297 Fumaric acid•E300 Ascorbic acid•E301 Sodium ascorbate•E302 Calcium ascorbate•E304 Fatty acid esters of ascorbic acid (i) Ascorbyl palmitate (ii) Ascorbyl stearate•E306 Tocopherol-rich extract•E307 Alpha-tocopherol•E308 Gamma-tocopherol•E309 Delta-tocopherol•E310 Propyl gallate•E311 Octyl gallate•E312 Dodecyl gallate•E315 Erythorbic acid•E316 Sodium erythorbate•E320 Butylated hydroxyanisole (BHA)•E321 Butylated hydroxytoluene (BHT)•E322 Lecithins•E325 Sodium lactate•E326 Potassium lactate•E327 Calcium lactate•E330 Citric acid•E331 Sodium citrates (i) Monosodium citrate (ii) Disodium citrate (iii) Trisodium citrate•E332 Potassium citrates (i) Monopotassium citrate (ii) Tripotassium citrate•E333 Calcium citrates (i) Monocalcium citrate (ii) Dicalcium citrate (iii) Tricalcium citrate•E334 Tartaric acid (L(+)-)•E335 Sodium tartrates (i) Monosodium tartrate (ii) Disodium tartrate•E336 Potassium tartrates (i) Monopotassium tartrate (ii) Dipotassium tartrate•E337 Sodium potassium tartrate•E338 Phosphoric acid•E339 Sodium phosphates (i) Monosodium phosphate (ii) Disodium phosphate (iii) Trisodium phosphate•E340 Potassium phosphates (i) Monopotassium phosphate (ii) Dipotassium phosphate (iii) Tripotassium phosphate•E341 Calcium phosphates (i) Monocalcium phosphate (ii) Dicalcium phosphate (iii) Tricalcium phosphate•E343 Magnesium phosphates (i) monomagnesium phosphate (ii) Dimagnesium phosphate [Under discussion and may be included in a future amendment to the Directive on miscellaneous additives]•E350 Sodium malates (i) Sodium malate (ii) Sodium hydrogen malate •E351 Potassium malate•E352 Calcium malates (i) Calcium malate (ii) Calcium hydrogen malate•E353 Metatartaric acid•E354 Calcium tartrate•E355 Adipic acid•E356 Sodium adipate•E357 Potassium adipate•E363 Succinic acid•E380 Triammonium citrate•E385 Calcium disodium ethylene diamine tetra-acetate (Calcium disodium EDTA)•E400 Alginic acid•E401 Sodium alginate•E402 Potassium alginate•E403 Ammonium alginate•E404 Calcium alginate•E405 Propan-1,2-diol alginate•E406 Agar•E407 Carrageenan•E407a Processed eucheuma seaweed [Added in December 1996 by Directive 96/83/EC]•E410 Locust bean gum•E412 Guar gum•E413 Tragacanth•E414 Acacia gum (gum arabic)•E415 Xanthan gum•E416 Karaya gum•E417 Tara gum•E418 Gellan gum•E420 Sorbitol (i) Sorbitol (ii) Sorbitol syrup•E421 Mannitol•E422 Glycerol•E425 Konjac (i) Konjac gum (ii) Konjac glucomannane[Added in October 1998 by Directive 98/72/EC]•E426 Soybean hemicellulose [Listed in proposed amendment in COM(2004)650 published October 2004]•E431 Polyoxyethylene (40) stearate•E432 Polyoxyethylene sorbitan monolaurate (polysorbate 20)•E433 Polyoxyethylene sorbitan monooleate (polysorbate 80)•E434 Polyoxyethylene sorbitan monopalmitate (polysorbate 40) •E435 Polyoxyethylene sorbitan monostearate (polysorbate 60) •E436 Polyoxyethylene sorbitan tristearate (polysorbate 65)•E440 Pectins (i) pectin (ii) amidated pectin•E442 Ammonium phosphatides•E444 Sucrose acetate isobutyrate•E445 Glycerol esters of wood rosins•E450 Diphosphates: (i) Disodium diphosphate (Disodium dihydrogen diphosphate, Disodium dihydrogen pyrophosphate, Sodium acidpyrophosphate, Disodium pyrophosphate); (ii) Trisodiumdiphosphate (Acid trisodium pyrophosphate, Trisodium monohydrogen diphosphate); (iii) Tetrasodium diphosphate (Tetrasodiumpyrophosphate, Sodium pyrophosphate); (iv) Dipotassiumdiphosphate (v) Tetrapotassium diphosphate (Potassiumpyrophosphate, Tetrapotassium pyrophosphate); (vi) Dicalcium diphosphate (Calcium pyrophosphate); (vii) Calcium dihydrogen diphosphate (Acid calcium pyrophosphate, Monocalcium dihydrogen pyrophosphate)•E451 Triphosphates: (i) Pentasodium triphosphate (pentasodium tripolyphosphate, sodium tripolyphosphate); (ii) Pentapotassium triphosphate (Pentapotassium tripolyphosphate, Potassiumtriphosphate, Potassium tripolyphosphate)•E452 Polyphosphates: (i) Sodium polyphosphates (Sodium hexametaphosphate, Sodium tetrapolyphosphate, Graham's salt, Sodium polyphosphates, glassy, Sodium polymetaphosphate, Sodium metaphosphate, Insoluble sodium metaphosphate, Maddrell's salt, Insoluble sodium polyphosphate, IMP); (ii) Potassiumpolyphosphates (Potassium metaphosphate, Potassiumpolymetaphosphate, Kurrol salt); (iii) Sodium calciumpolyphosphate (Sodium calcium polyphosphate, glassy); (iv) Calcium polyphophates (Calcium metaphosphate, Calcium polymetaphosphate)•E459 Beta-cyclodextrine [Added in October 1998 by Directive 98/72/EC]•E460 Cellulose (i) Microcrystalline cellulose (ii) Powdered cellulose•E461 Methyl cellulose•E462 Ethyl cellulose [Listed in proposed amendment in COM(2004)650 published October 2004]•E463 Hydroxypropyl cellulose•E464 Hydroxypropyl methyl cellulose•E465 Ethyl methyl cellulose•E466 Carboxy methyl cellulose, Sodium carboxy methyl cellulose•E467 Ethyl hydroxyethyl cellulose [Number had been allocated in a proposed amendment to Directive 95/2 circulated in August 1999. However, it was not accepted and further evaluation was requested]•E468 Crosslinked sodium carboxymethyl cellulose [Added in October 1998 by Directive 98/72/EC]•E469 Enzymically hydrolysed carboxy methyl cellulose[Added in October 1998 by Directive 98/72/EC]•E470a Sodium, potassium and calcium salts of fatty acids•E470b Magnesium salts of fatty acids•E471 Mono- and diglycerides of fatty acids•E472a Acetic acid esters of mono- and diglycerides of fatty acids •E472b Lactic acid esters of mono- and diglycerides of fatty acids •E472c Citric acid esters of mono- and diglycerides of fatty acids •E472d Tartaric acid esters of mono- and diglycerides of fatty acids •E472e Mono- and diacetyl tartaric acid esters of mono- and diglycerides of fatty acids•E472f Mixed acetic and tartaric acid esters of mono- and diglycerides of fatty acids•E473 Sucrose esters of fatty acids•E474 Sucroglycerides•E475 Polyglycerol esters of fatty acids•E476 Polyglycerol polyricinoleate•E477 Propane-1,2-diol esters of fatty acids•E479b Thermally oxidized soya bean oil interacted with mono- and diglycerides of fatty acids•E481 Sodium stearoyl-2-lactylate•E482 Calcium stearoyl-2-lactylate•E483 Stearyl tartrate•E491 Sorbitan monostearate•E492 Sorbitan tristearate•E493 Sorbitan monolaurate•E494 Sorbitan monooleate•E495 Sorbitan monopalmitate•E500 Sodium carbonates (i) Sodium carbonate (ii) Sodium hydrogen carbonate (iii) Sodium sesquicarbonate•E501 Potassium carbonates (i) Potassium carbonate (ii) Potassium hydrogen carbonate•E503 Ammonium carbonates (i) Ammonium carbonate (ii) Ammonium hydrogen carbonate•E504 Magnesium carbonates (i) Magnesium carbonate (ii) Magnesium hydroxide carbonate (syn. Magnesium hydrogen carbonate)•E507 Hydrochloric acid•E508 Potassium chloride•E509 Calcium chloride•E511 Magnesium chloride•E512 Stannous chloride•E513 Sulphuric acid•E514 Sodium sulphates (i) Sodium sulphate (ii) Sodium hydrogen sulphate•E515 Potassium sulphates (i) Potassium sulphate (ii) Potassium hydrogen sulphate•E516 Calcium sulphate•E517 Ammonium sulphate•E520 Aluminium sulphate•E521 Aluminium sodium sulphate•E522 Aluminium potassium sulphate•E523 Aluminium ammonium sulphate•E524 Sodium hydroxide•E525 Potassium hydroxide•E526 Calcium hydroxide•E527 Ammonium hydroxide•E528 Magnesium hydroxide•E529 Calcium oxide•E530 Magnesium oxide•E535 Sodium ferrocyanide•E536 Potassium ferrocyanide•E538 Calcium ferrocyanide•E541 Sodium aluminium phosphate, acidic•E551 Silicon dioxide•E552 Calcium silicate•E553a (i) Magnesium silicate (ii) Magnesium trisilicate•E553b Talc•E554 Sodium aluminium silicate•E555 Potassium aluminium silicate•E556 Calcium aluminium silicate•E558 Bentonite•E559 Aluminium silicate (Kaolin)•E570 Fatty acids•E574 Gluconic acid•E575 Glucono-delta-lactone•E576 Sodium gluconate•E577 Potassium gluconate•E578 Calcium gluconate•E579 Ferrous gluconate•E585 Ferrous lactate•E586 4-hexylresorcinol [Listed in proposed amendment in COM(2004)650 published October 2004]•E620 Glutamic acid•E621 Monosodium glutamate•E622 Monopotassium glutamate•E623 Calcium diglutamate•E624 Monoammonium glutamate•E625 Magnesium diglutamate•E626 Guanylic acid•E627 Disodium guanylate•E628 Dipotassium guanylate•E629 Calcium guanylate•E630 Inosinic acid•E631 Disodium inosinate•E632 Dipotassium inosinate•E633 Calcium inosinate•E634 Calcium 5'-ribonucleotides•E635 Disodium 5'-ribonucleotides•E640 Glycine and its sodium salt•E650 Zinc acetate[Added in February 2001 by Directive 2001/5/EC]•E900 Dimethyl polysiloxane•E901 Beeswax, white and yellow•E902 Candelillla wax•E903 Carnauba wax•E904 Shellac•E905 Microcrystalline wax [Added in October 1998 by Directive 98/72/EC]•E907 Hydrogenated poly-1-decene [Added in December 2003 by Directive 2003/114/EC]•E912 Montanic acid esters•E914 Oxidized polyethylene wax•E920 L-Cysteine [Added in October 1998 by Directive 98/72/EC]•E927b Carbamide•E938 Argon•E939 Helium•E941 Nitrogen•E942 Nitrous oxide•E943a Butane[Added in February 2001 by Directive 2001/5/EC]•E943b Isobutane [Added in February 2001 by Directive 2001/5/EC]•E944 Propane [Added in February 2001 by Directive 2001/5/EC]•E948 Oxygen•E949 Hydrogen [Added in February 2001 by Directive 2001/5/EC]•E950 Acesulfame K•E951 Aspartame•E952 Cyclamic acid and its Na and Ca salts•E953 Isomalt•E954 Saccharin and its Na, K and Ca salts•E955 Sucralose [Added in December 2003 by Directive 2003/115]•E957 Thaumatin•E959 NeohesperidineDC•E962 Salt of aspartame - acesulfame [Added in December 2003 by Directive 2003/115]•E965 Maltitol (i) Maltitol (ii) Maltitol syrup•E966 Lactitol•E967 Xylitol•E968 Erythritol [Listed in proposed amendment in COM(2004)650 published October 2004]•E999 Quilllaia extract•E1103 Invertase [Added in October 1998 by Directive 98/72/EC]•E1105 Lysozyme•E1200 Polydextrose•E1201 Polyvinylpyrrolidone•E1202 Polyvinylpolypyrrolidone•E1404 Oxidized starch•E1410 Monostarch phosphate•E1412 Distarch phosphate•E1413 Phosphated distarch phosphate•E1414 Acetylated distarch phosphate•E1420 Acetylated starch•E1422 Acetylated distarch adipate•E1440 Hydroxy propyl starch•E1442 Hydroxy propyl distarch phosphate•E1451 Acetylated oxidised starch[Added in October 1998 by Directive 98/72/EC]•E1450 Starch sodium octenyl succinate•E1505 Triethyl citrate•E1517 Glyceryl diacetate (diacetin)•E1518 Glyceryl triacetate (triacetin)•E1519 Benzyl alcohol•E1520 Propan-1,2-diol (propylene glycol)[Added in February 2001 by Directive 2001/5/EC]。
Luc-Pair
Luc-Pair™Duo-Luciferase HS Assay Kit-高灵敏性双荧光素酶检测试剂盒Cat.No.LF004(100reactions)Cat.No.LF005(300reactions)Cat.No.LF006(1000reactions)使用说明书GeneCopoeia,Inc.广州易锦生物技术有限公司9620Medical Center Drive,#101地址：广州科学城揽月路3号F区F801（510663）Rockville,MD20850电话：4006-020-200、************、************ USA网站：301-762-0888866-360-9531***********************©2016GeneCopoeia,Inc.使用说明书Luc-Pair™Duo-Luciferase HS Assay KitI.产品概述II.产品信息及储存条件III.细胞裂解IV.FLuc和RLuc工作液的配制V.荧光素酶检测流程VI.有限使用许可及质保声明I.产品概述对报告基因表达的转录调控的研究常被应用于生物学研究和药物发现。
荧光素酶在基因表达研究中应用最为广泛，其主要包含以下几个优点：1)在广泛动态范围内具有高灵敏度2)在哺乳动物细胞内无荧光素酶、背景极低3)实验重复性好4)成本低5)操作简单萤火虫和海肾荧光素酶都具有快捷、简便、灵敏度高的检测特点，被公认为是理想的报告基因，因为它们具有完全不同的进化起源、酶学结构和底物要求。
萤光素酶报告基因的测定需要用光度计或多功能微孔板检测仪，且发光强度与荧光素酶的数量成正比。
萤火虫荧光素酶（Photinus pyralis）已被证实是检测启动子活性和监测基因转录后调控状态的理想的报告基因。
它是在细胞质中作用的酶，分子量为61kDa并催化下列反应：海肾（Renilla reniformis）荧光素酶是一个36kDa单亚基蛋白质，酶活性不需要翻译后修饰，因此它可以作为一个实时转录报告基因，催化下面的生物发光反应：此体系可以在目的基因的附近监控顺式作用元件的转录激活。
SurePrep
BP2816-50BP2805-50BP2802-50BP2801-25BP2800-50BP2806-50BP2807-50BP2809-50BP2803-50BP2804-50FFPE RNA CytoplasmicRNA RNA, DNA,and ProteinSmall RNA T rueT otalRNARNA andProteinLeukocyteRNARNA CleanupandConcentrationUrineExfoliatedCellUrineBacterialRNARNA X X X X X X X X X microRNA X X X X X X X X X X DNA XProtein X XLaboratory ApplicationsAgricultural X X XEnvironmental X X XFood Science X X XForensicPathologyand BiologyX X XHistology XIndustrial andPublic HealthX X X X XInfectiousDiseaseDetectionX X X X XIndustriesMedicalResearchX X X X XAcademicResearchX X X X XGovernmentResearchX X X X XBiotech ResearchX X X X XCONTENTSSurePrep Application Summary Chart (2)SurePrep FFPE RNA Isolation Kit (3)SurePrep Nuclear or Cytoplasmic RNA Puri cation Kit (4)SurePrep RNA/DNA/Protein Puri cation Kit (5)SurePrep Small RNA Puri cation Kit (6)SurePrep TrueT otal™ RNA Puri cation Kit (7)SurePrep RNA/Protein Puri cation Kit (8)SurePrep Leukocyte RNA Puri cation Kit (9)SurePrep RNA Cleanup and Concentration Kit (10)SurePrep Urine Exfoliated Cell RNA Puri cation Kit (11)SurePrep Urine Bacterial RNA Puri cation Kit (11)SurePrep FFPE RNA ISOLATION KIT - SPECIFICATIONSSPINSPINSPIN Elute RNWash RNAPuri ed T* The RNA fragments puri ed depend on the age of the FFPE tissue as the degree of fragmentation ofthe RNA will increase over time.KIT SPECIFICATIONS:Column Binding Capacity......................................50µgMaximum Amount of Sample ..............................5 paraf n slices, 20µm thickUnsectioned block: 25mgSize of RNA Purifi ed rge, small and micro.Lysis Method ...................................................................Final Elution VolumeNo. of Buffers .................................................................Time to Complete ProcessNo. of Preps Cat. No.Description50BP2816-50SurePrep FFPE RNA Isolation PuriNote: Longer time is required to suffi ciently isolate all functionally intact total RNA species from apreserved sample in order to maximize yield.KIT COMPONENTS:Lysis Solution, 20mL; RNA WashSolution, 22mL; Binding Solution, 25mL; RNA Elution Buffer, 6mL; Spin Columns, 50; Collection T ubes, 50; 1.7mL Elution T ubes, 50; Product InsertRECOMMENDED STORAGE:All solutions should be kept tightly sealed All sizes, including small RNA (<200 nt)600µL 3 x 106 cells 15mg45 minutes for 10 puri cations Centrifuge to clear lysate 1. Bind RNA3. Elute RNA3. E 2. Wash RNAAdd Ethanol Centrifuge to clear lysate 1. Bind RNA3. Elute RNA3. E 2. Wash RNACentrifuge to separate cell fractionsCytoplasmic RNA50µg for RNA20µg for DNA200µg for protein600µLAll sizes, including small RNA (<200 nt)≥30kb3 x 106 cellsKIT COMPONENTS:Lysis Solution, 40mL; RNA Wash Solution, 20mL;RNA Elution Solution, 6mL; gDNA Wash Solution, 15mL; gDNA Elution Buffer, 15mL; Protein Column Regeneration Buffer, 30mL; Protein ColumnActivation and Wash Buffer, 60mL; Protein pHBinding Buffer, 4mL; Protein Elution Buffer, 8mL;Protein Neutralizer, 2mL; Protein Loading Dye,2mL; Spin Columns, 50; Collection tubes, 200;Bind Proteinsto ActivatedColumnWashEluteProteinsProteinsBindto AColuBind RNA andDNA to ColumnWashElute RNABindDNAFlowthroughWash DNAElute gDNAgDNANAKIT SPECIFICATIONS:Column Binding Capacity ......................................50µg Maximum Column Loading Volume ................600µL Size of RNA Purifi ed ................................................<200 nt Maximum Amount of Starting MaterialAnimal Cells ................................................................ Animal T issues .......................................................... Bacteria .......................................................................... Plant T issues ...............................................................Time to Complete PurifiSurePrep SMALL RNA PURIFICATION KIT - SPECIFICATIONS6♦♦Elute small RNA with Elution BufferBind large RNAmolecules to Large RNA Removal ColumnBind small RNA molecules from Small RNA Enrichment ColumnWash three times with Puri ed Small RNANo. of Preps Cat. No.Description25BP2801-25SurePrep Small RNA PuriKIT COMPONENTS:Lysis Solution, 40mL; RNA Wash Solution, 22mL; RNA Elution Buffer, 6mL; Micro Spin Columns, 50; Collection T ubes, 50; 1.7mL Elution T ubes, 50; Product Insert RECOMMENDED STORAGE: Store at room temperature.KIT SPECIFICATIONS:Column Binding Capacity ......................................50µg Maximum Column Loading Volume ................600µL Size of RNA Purifi ed ................................................All sizes, including small RNA (<200 nt)Maximum Amount of Starting MaterialAnimal Cells ................................................................3 x 106 cells Animal T issues ..........................................................25mg Blood .................................................................................100µLBacteria ..........................................................................1 x 109 cells Y east ..................................................................................1 x 108 cells Fungi .................................................................................50mg Plant T issues ...............................................................50mgTime to Complete Purifi cation Process .....20 minutes for 10 puri cations Average Y ields from RNA Purifi cationHeLa cells (1 x 106cells) ......................................15µg E. coli (1 x 109 cells) .................................................50µg SurePrep TrueT otal RNA PURIFICATION KIT - SPECIFICATIONS7No. of PrepsCat. No.Description50BP2800-50SurePrep TrueT otal RNA Puri cation KitKIT COMPONENTS:Lysis Solution, 40mL; Nucleic Acid Wash Solution, 22mL; Nucleic Acid ElutionSolution, 15mL; Protein Column Regenera-tion Buffer, 30mL; Protein ColumnActivation and Wash Buffer, 60mL; Protein pH Binding Buffer, 4mL; Protein Elution Buffer, 8mL; Enzyme Incubation Buffer, 6mL; Protein Neutralizer, 2mL; Protein Loading Dye, 2mL; Spin Columns, 50; Collection tubes, 150; Product Insert RECOMMENDED STORAGE:The Protein Loading Dye should be stored at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature.KIT SPECIFICATIONS:Column Binding Capacity ......................................50µg RNA200µg for proteinMaximum Column Loading Volume ................600µL Size of RNA Purifi ed ................................................All sizes, including small RNA (<200 nt)Maximum Amount of Starting MaterialAnimal Cells ................................................................3 x 106 cells Animal T issues ..........................................................25mg Blood .................................................................................100µLcations SurePrep RNA/PROTEIN PURIFICATION KIT - SPECIFICATIONSProteinsRNA Bind RNAto ColumnWash Elute RNAWash RNARN Bind to Co FlowthroughBind Proteins to Activated Column Elute Proteins Elute ProtWashBind to Ac ColumColKIT COMPONENTS:RBC Lysis Solution, 180mL; Binding Solution, 25mL; Wash Solution, 22mL;RNA Elution Buffer, 6mL; Spin Columns, 50; Collection T ubes, 50; 1.7mL Elution KIT SPECIFICATIONS:Column Binding Capacity ......................................50µg Maximum Column Loading Volume ................600µL Size of RNA Purifi ed ................................................All sizes, including small RNA (<200 nt)Maximum Amount of Starting MaterialMaximum Blood Input ...........................................2mL or 3 x 10 Minimum Blood Input ............................................10µLTime to Complete Purifi cation Process .....45 minutes for 10 puri Average Y ields from RNA Purifi cation500µL human blood ..............................................1.5µgSurePrep LEUKOCYTE RNA PURIFICATION KIT - SPECIFICATIONSclear lysateBind RNAWash RNAElute RNAWash Bind EluteKIT SPECIFICATIONS:Column Binding Capacity ......................................50µg Maximum Column Loading V olume ................600µL Size of RNA Purifi ed ................................................All sizes, including small RNA (<200 nt)Maximum Amount of Starting Material .......50µg of RNA Minimum Elution V olume ........................................20µL Time to Complete Purifi cation Process .....20 minutes for 10 puri cations Average Recovery .......................................................≥ 90%10SurePrep RNA CLEANUP AND CONCENTRATION KIT - SPECIFICATIONSNo. of Preps Cat. No.Description50BP2809-50SurePrep RNA Cleanup and Concentration KitKIT SPECIFICATIONS:Column Binding Capacity .....................................50μgVolume of Urine Processed .................................1-50mL, Exfoliated Cell Kit (BP2803-50)10-50mL, Bacterial Kit (BP2804-50)Maximum Input of Exfoliated Cells ........... .....1 x 106 cells Exfoliated Cell Kit (BP2803-50)*Yield varies due to cell density of sample.SurePrep URINE EXFOLIATED CELL andSurePrep URINE BACTERIAL RNA PURIFICATION KITS - SPECIFICATIONSPellet bacterial cells Bind RNA to columnWash Elute RNA Urine Sample otal Urine Bacterial RNAElutWasBin colu and lysis solution. Add EthanolCat. No.DescriptionBP2804-50SurePrep Urine Bacterial RNA Puri Cat. No.DescriptionBP2803-50SurePrep Urine Exfoliated Cell RNA PuriAMERICASCanadaFisher Scientifi c Canada112 Colonnade RoadOttawa, OntarioPost Code: K2E 7L6Toll-Free Number: 800-234-7437Fax: 800-463-2996www.fi shersci.caLatin AmericaFisher Scientifi c Global Export, Latin America 3970 Johns Creek CourtSuite 500Suwanee, GAPost Code: 30024Toll-Free Number: 770-871-4725Fax: 770-871-4726www.fi IndiaFisher Scientifi c India101A-101B, Godrej Coliseum,Somaiya Hospital Road,Off Eastern Express Highway,Sion East, Mumbai 400 022CustomerServiceTollFree:180****7001Fax: 022 6680 3001 or 3002qfc.customercare@thermofi www.fi shersci.inJapanFisher Scientifi c JapanThermo Fisher Scientifi c K.K.C-2F, 3-9 Moriya-choKanawaga-ku, Yokohama221-0022 JapanTel: 81 45 450 6310Fax: 81 45 450 6316support@fi shersci.co.jpCustomer Service Center: (02) 527-0300c Malaysia Sdn BhdTaman Perindustrian Axis Seksyen 25Technical Service Hotline: 1-300-88-7868BelgiumFisher Scientifi cBP 567B-7500 Tournai 1Tel: 056 260 260Fax: 056 260 270be.fi sher@thermofi www.be.fi Czech RepublicFisher Scientifi c, spol. s r.o.Kosmonautu 324PardubiceCZ-530 09Tel: 466 798 230Fax: 466 435 008info.cz@thermofi www.thermofi sher.czDenmarkFisher Scientifi c Biotech Line A/SIndustrivej 3Postboks 60DK-3550 SlangerupTel: +45 70 27 99 20Fax: +45 70 27 99 29kundeservice@thermofi www.fi shersci.dkFranceFisher Scientifi cParc d’Innovation BP 5011167403 Illkirch CedexTel: 03 88 67 53 20Fax: 03 88 67 11 68mande@thermofi www.fr.fi GermanyFisher Scientifi c GmbHIm Heiligen Feld 17Blanchardstown CorporateBallycoolinDublin 15Tel: +353 01 885 5854Fax: +353 01 899 1855fsie.sales@thermofi www.ie.fi Fisher Scientifi cTel: 02 953 28 258Fax: 02 953 27 374sher@thermofi www.it.fi The NetherlandsFisher Scientifi cPostbus 4, Scheepsbouwersweg 1BNorwayFisher Scientifi cFrysjaveien 33E0884 OsloTel: +47 22 95 59 59Fax: +47 22 95 59 40fi sher.no@thermofi www.fi shersci.noSpainFisher Scientifi cVía de los poblados, 17 Nave 3-1328033 MadridTel: 91 515 92 34Fax: 91 515 92 35es.fi sher@thermofi www.es.fi SwedenFisher Scientifi cBox 9193400 94 GöteborgTel: +46 31 - 68 94 30Fax: +46 31 - 68 07 17@thermofi www.fi shersci.seSwitzerlandFisher Scientifi cWilstrasse 57 - Postfach 10065610 WohlenTel: 056 618 41 11Fax: 056 618 41 41info.ch@thermofi www.ch.fi United KingdomFisher Scientifi c UK LtdBishop Meadow RoadLoughboroughLeicestershire LE11 5RG2240 Geel – BelgiumTel: +32 14 57 52 11Fax: +32 14 59 26 10MIDDLE EAST AND AFRICAFisher Scientifi c Global Export,Latin America3970 Johns Creek CourtSuite 500Suwanee, GAPost Code: 30024Toll-Free Number: 770-871-4725Fax: 770-871-4726www.fi ©2009 Thermo Fisher Scientific Inc.All rights reserved. Litho in U.S.A.09_2059 DC/LJ 10M-IW-5/09GLOBAL LOCATIONS。
【材料】印刷材料学PPT课件
d : 苯的密度
.
32
二、平均孔半径 指长度等于纸页厚度之孔隙的当量半径 仅表示孔隙量的相对大小，并不能说明孔
隙的大小、形状和分布 经测定发现：
沿纸页纵向的平均孔径半径最大 沿厚度方向的平均孔径半径最小 三、孔径分布 孔径分布对于印刷纸的重要性
.
33
第三章 纸和纸板的基本物理性能
学性能 3、 匀度的测量
MK Formation Analyzers: Drum Scanner Model MK975
.
24
第三节 纸张的水平结构及垂直结构
一、纸张的水平结构
纸页的力学性质不仅取决于纤维间的 接触，即纤维间交叉总量，还取决于纤 维网络中每根纤维的交叉数量。
.
25
Microscopic image of paper surface
纸张表面 微观结构
.
26
Two-dimensional random fiber network approximation excluding the free fiber ends.
.
27
二、纸张的垂直结构：
1、纤维间粘结的程度——垂直结构中最重 要的结构性质
Z向强度：纸张在垂直方向的抗张强度
第一篇 承印材料
第一章 印刷纸的组成
第一节 印刷纸的基本组成——植物纤维 一、造纸植物纤维原料的种类 1、木材纤维原料 2、非木材纤维原料
.
1
几种纤维的比较
.
亚麻 木 棉 丝 微细纤维
2
二、造纸纤维原料的化学组成及特点
1、纤维素 2、半纤维素 3、木素 4、木材、草类和棉纤维的组成特点
.
3
染色及助剂环保标准--2009年要求
邻氨基偶氮甲苯
97-56-3
４氨基联苯
92-67-1
５硝基邻甲苯胺
99-55-8
邻氨基苯甲醚
90-04-0
联苯胺
92-87-5
对氯苯胺
106-47-8
４氯邻甲苯胺
95-69-2
２甲氧基５甲基苯胺
120-71-8
２，４二氨基苯甲醚
615-05-4
４，４二氨基二苯甲烷
2682-20-4
五氯化苯酚(PCP)
87-86-5
未检出
§64 LFGB B 82.02.8(RL=0.5)
四氯苯酚(TeCP)
25167-83-3
富马酸二甲酯
624-49-7
禁止使用
溶剂萃取/GC-MS
IV) Bisphenol-A (BPA)双酚A
化学物质
CAS编号
产品最高限量
(ppm)
检检法
4,4dihydroxy2,2diphenylpropane
80-05-7
未检出
GC-MS/
LC-MS
V)抗光敏性致癌的分散染料及其它染料
化学物质
CAS编号
产品最高限量
检测法
(ppm)
分散染料
未检出
DIN54231单位：
毫克/升（RL：5）
分散蓝1
2475-45-8
分散蓝3
2475-46-9
分散蓝7
4098-71-9
游离型:1
封闭型: 100
四甲基苯二甲基二异氰酸酯
(TMXDI)
2778-42-9
游离型:1
封闭型: 100
甲苯异氰酸酯
(TDI)
iClick EdU Andy Fluor 647 Flow Cytometry Assay Kit
iClick™ EdU Andy Fluor 647 Flow Cytometry Assay Kit ——EdU法细胞增殖流式检测试剂盒产品货号包装规格A008 250次储存条件：-20℃，避光保存。
激发/发射波长：Andy Fluor 647 azide: 650/665 nm。
产品说明书GeneCopoeia, Inc. 广州易锦生物技术有限公司广州高新技术产业开发区广州科学城掬泉路3号广州国际企业孵化器F区8楼邮编：510663电话：4006-020-200邮箱：******************网址：（英文）（中文）© 2016 GeneCopoeia, Inc.iClick™ EdU Andy Fluor 647 Flow Cytometry Assay Kit产品货号: A008试剂盒组份：组份试剂名称体积/质量浓度储存温度组份A EdU 2×1 ml 10 mM in DMSO -20℃组份B Andy Fluor 647 azide 150 µl NA -20℃，避光保存组份C iClick fixative 5 ml 1×solution 4℃50 ml 10×solution 4℃组份D iClick permeabilizationand wash reagent组份E CuSO4 1 ml 100 mM in H2O 4℃组份F iClick EdU buffer additive 200 mg NA 4℃注：按照推荐的储存条件保存有效期为1年，请注意避免反复冻融。
产品介绍直接测定DNA合成是细胞增殖检测的最准确方法之一，是测定物质毒性、评估药物安全评价、细胞健康的基本方法，其中以前常用的方式是利用胸腺嘧啶核苷酸类似物-BrdU进行检测。
因为在细胞周期的S期，和细胞一起孵育的BrdU能掺入DNA 分子中，再结合BrdU抗体与掺入DNA的BrdU特异性结合，就能够检测到DNA 复制活跃的细胞。
伐地那非杂质
CAS No.: 448184-461 Molecular Formula: C21H28N6O4S Molecular Weight: 460.56
结构式
H&D
N-Desethyl Vardenafil
H&D
Varden915 Molecular Formula: C23H32N6O4S HCl Molecular Weight: 488.61 36.46
H&D
Hydroxy Vardenafil
Molecular Formula: C23H32N6O5S Molecular Weight: 504.61
H&D
Vardenafil Impurity 3
Molecular Formula: C21H28N6O4S Molecular Weight: 460.56
H&D
Vardenafil Impurity CAS No.: 879-57-2 Molecular Formula: (2-Ethoxy-NC9H12N2O2 Hydroxy Molecular Weight: Benzamidine) 180.21
H&D
Vardenafil Benzoyl Molecular Formula: C26H30N6O4S Impurity Molecular Weight: 522.63
H&D
Acetylvardenafil
Molecular Formula: C25H34N6O3 Molecular Weight: 466.59
Molecular Formula: C38H46N10O8S2 Molecular Weight: 834.98
Thuricide BT 虫害控制剂说明书
1Distributed By:Southern Agricultural Insecticides, Inc.PALMETTO, FL. 34220 HENDERSONVILLE, N.C. 28793 BOONE, N.C. 28607EPA REG. NO. 70051-106-829 J1 EPA Est. No. 829-FL-1 Net Contents Liquid: 1 Gallon (3.785 liters)THURICIDE BT CATERPILLAR CONTROL For Home and Gardens Controls worms and caterpillars on Fruits,Vegetables, Ornamentals and Shade TreesKEEP OUT OF REACH OF CHILDRENCAUTION See inside of label for complete warnings and directions for use.Follow Directions on side of label to open.For information and questions concerning this product please *************************************.ACTIVE INGREDIENT:Bacillus thuringiensis Subsp. Kurstaki strainSA-12 solids, spores and lepidopteran activetoxins ( At least 6 million viable sporesper mg*)......................................... 98.35%OTHER INGREDIENTS ................ 1.65%Total................................................100.00%*The percent active ingredient does not indicate productperformance and potency measurements are not federallystandardized.Thuricide is a registered trademark of Certis USAOrganic MaterialsReview InstitutePRECAUTIONARY STATEMENTSHAZARDS TO HUMANS AND DOMESTIC ANIMALSCAUTION: Causes moderate eye irritation. Avoid contact eyes or clothing. Avoid breathing vapors or spray mist. Wash thoroughly with soap and water after handling.ENVIRONMENTAL HAZARDSTo protect the environment, do not allow pesticide to enter or run off into storm drains, drainage ditches, gutters or surface waters. Applying this product in calm weather when rain is not predicted for the nest 24 hours will help to ensure that wind or rain does not blow or wash pesticide off the treatment area. Rinsing ap-plication equipment over the treated area will help avoid run off to water bodies or drainage systems.FIRST AIDIF IN EYES: Hold eye open and rinse slowly and gently with water for 15-20 minutes. Remove contact lenses, if present, after the first 5 minutes, then continue rinsing eye. Call a poison control center or doctor for treatment advice.Have product container or label with you when calling a poison control center or doctor or going for treatment. Hot Line Number: 1-800-255-3924.Thuricide is an easy to mix liquid concentrate. Properties include:* Kills worms and caterpillar stage insects, but has no effect on birds, earthworms, or beneficial insects, such as honeybees and ladybugs, when used as directed.* W orms and caterpillars eat treated foliage, then immediately stop feeding and damaging plants.* A cceptable for use on edible plants up to the day of harvest.2DIRECTIONS FOR USEIt is a violation of Federal Law to use this product in a manner inconsistent with its labeling. Apply Thuricide BT Caterpillar Control when worms or caterpillars are first noticed, then repeat at five (5) to seven (7) day intervals while they are active. Apply more frequently to control heavy infestations. Apply thoroughly to top and bottom of foliage. Reapply after heavy rains.Thuricide BT Caterpillar Control must be eaten by worms or caterpillars to be effective. After ingesting the insecticide, they immediately stop feeding, though they may otherwise appear to be unaffected for several days. Best results are obtained by treatments when worms are small; they must be actively feeding on treated, exposed foliage. You may apply Thuricide BT Caterpillar Control up to and on the day of harvest.How to apply: Always shake or stir Thuricide BT Caterpillar Control thoroughly before use. Partially fill sprayer with water before adding the application rate amount of product, then mix in product thoroughly and add remaining amount of water.Spray leaf surfaces thoroughly, top and bottom for complete control. Agitate regularly while spraying. Use all of spray mixture within 24 hours.Fruits and vegetables: mix 1.5 fluid ounces per 3 gallons water (1 tablespoon per gal-lon) for a hand sprayer and apply to all plant foliage to cover an area of 1,000 square feet. Apply at first sign of infestation and repeat at weekly intervals when needed to maintain control of the following insects:Cabbage Looper on broccoli, cauliflower, collards, kale, mustard greens, turnip greens, cabbage, celery, lettuce, melons and tomatoes.Imported Cabbage Worm: On broccoli, cabbage, cauliflower, collards, kale, mustard greens and turnip greens.Tomato Hornworms on tomatoes.3Shade Trees & Ornamentals: Mix 2 fluid ounces per 3 gallons of water (4 teaspoons per gallon) for a hand sprayer or proportional amounts for a hose-end sprayer following manu-facturer’s directions. Apply at first sign of infestation and repeat at weekly intervals when needed to maintain control. Apply to thoroughly cover all foliage surfaces of shade trees and ornamentals for control of the following leaf-feeding worms:Bagworm Spring Cankerworm Fall CankerwormGypsy Moth* Tent Caterpillar Elm SpanwormFall Webworm* Apply when buds swell, but before leaves are fully open. If eggs hatch over a long period of time or if reinfestation occurs, spray about 14 days after first application.STORAGE AND DISPOSALPESTICIDE STORAGE:Store in a cool place.Insect activity may be impaired by storage at temperatures above 90 degrees Fahrenheit. PESTICIDE DISPOSAL and CONTAINER HANDLING:Nonrefillable container. Do not reuse or refill this container.If empty:Place in trash or offer for recycling if available.If partly filled:Call your local solid waste agency or 1-800-CLEANUP for disposal instructions.Never place unused product down any indoor or outdoor drain.4WARRANTYSouthern Agricultural Insecticides, Inc. warrants that the material contained herein con-forms to the description on the label and is reasonably fit for the purposes referred to in the directions for use. Timing and method of application, weather, watering practices, nature of soil, the insect problem, condition of the crop, incompatibility with other chemicals not specifically recommended, and other influencing factors in the use of this product are beyond the control of the seller. Buyer assumes all risks of use, storage or handling of the material not in strict accordance with directions given herein. NO OTHER EXPRESS OR IMPLIED WARRANTY OF THE FITNESS OF MERCHANTABILITY IS MADE.If any part of this label is missing, contact your supplier or contact us at ****************************************************.Thuricide BT Caterpillar ControlEPA Reg. No. 70051-106-829Southern Agricultural Insecticides, Inc.Palmetto, Fl 342207。
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Product Name:
Carfilzomib CAS No.:
868540-17-4Cat. No.:
HY-10455
Product Data Sheet
MWt:
719.91Formula:
C40H57N5O7Purity :>98%
Solubility:
DMSO 50 mg/mL
Mechanisms:
Biological Activity:
Carfilzomib (PR 171)is an irreversible proteasome inhibitor with IC50of <5nM Pathways:Metabolism/Protease; Target:Proteasome
Carfilzomib (PR-171) is an irreversible proteasome inhibitor with IC50 of <5 nM.
IC50: < 5 nM [1]Carfilzomib, an irreversible proteasome inhibitor, has a favorable safety profile and significant anti-tumor activity in patients with relapsed and refractory multiple myeloma (MM). Here we summarize the clinical pharmacokinetics (PK), metabolism, and drug-drug interaction (DDI) profile of
carfilzomib. The PK of carfilzomib, infused over 2-10 min, was evaluated in patients with solid tumors or MM. The proteasome has emerged as an important target for cancer therapy with the approval of bortezomib, a first-in-class, reversible proteasome inhibitor, for relapsed/refractory
multiple myeloma (MM). Carfilzomib induced a dose- and time-dependent inhibition of proliferation,References:
[1]. Kuhn DJ, Chen Q, Voorhees PM et al. Potent activity of carfilzomib, a novel, irreversible inhibitor of the ubiquitin-proteasome pathway, against preclinical models of multiple myeloma. Blood. 2007
Nov 1;110(9):3281-90.p y ()p p ultimately leading to apoptosis. Carfilzomib showed increased efficacy compared with bortezomib and was ac...
;()[2]. Dasmahapatra G, Lembersky D, Son MP et al. Carfilzomib interacts synergistically with histone deacetylase inhibitors in mantle cell lymphoma cells in vitro and in vivo. Mol Cancer Ther. 2011
Sep;10(9):1686-97.[3]. Siegel DS, Martin T, Wang M et al. A phase 2 study of single-agent carfilzomib (PX-171-003-A1)
in patients with relapsed and refractory multiple myeloma. Blood. 2012 Oct 4;120(14):2817-25.
[4]. Closed Clinical Trials with Carfilzomib Caution: Not fully tested. For research purposes only
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18 W i l k i n s o n W a y , P r i n c e t o n , N J 08540,U S A
E m a i l : i n f o @m e d c h e m e x p r e s s .c o m W e b : w w w .m e d c h e m e x p r e s s .c o m。