牵引力介导MC3T3

牵引力介导MC3T3⁃E1细胞microRNA的差异表达
孙芬;刘铭;刘名燕
【期刊名称】《口腔医学》
【年(卷),期】2018(038)003
【摘要】目的探讨周期性牵引力作用下小鼠成骨前体细胞microRNA的表达谱,寻找与TGF?β信号通路相关的差异mi?croRNA.方法体外培养 MC3T3?E1细胞.以正常培养细胞为对照,应力加载组细胞加载12%拉伸应变力3 h,利用microRNA 基因芯片技术初步筛选出牵张应力作用下MC3T3?E1细胞差异表达的microRNA,然后采用实时荧光定量PCR技术对与TGF?β信号通路相关的差异表达microRNAs进行验证,生物信息学预测可能受其调控的靶基因.结果与对照组相比,应力加载组有41个microRNAs表达差异(P值均小于0.05且差异倍数均大于1.5倍),其中20个表达上调,21个表达下调.实时荧光定量PCR结果显示:相比于对照组,应力加载组中miR?132?3p的表达水平升高,与芯片结果一致且存在统计学差异(P<0.05).经软件分析,miR?132?3p的靶基因可能为Smad2.结论周期性牵引力作用下成骨细胞microRNA表达谱发生改变,这些差异表达的microRNAs可能通过影响TGF?β信号通路或其相关信号分子来影响成骨细胞的生物学功
能.%Objective To investigate the profile of microRNA expression in mouse pre?osteoblast cell under cyclic stretch and to find the differences in microRNA which are related to TGF?β signaling pathway. Methods
MC3T3?E1 cells were cultured in vitro. Normal cells were considered as controls and loading group cells loaded with 12% elongation for 3 hours,the differential expressed microRNA of MC3T3?E1 cell under
mechanical strain conditions was screened by microRNA Microarray Technology,and then the Quantitative Re?al?time PCR was used to verify the differences associated with TGF?β signaling pathway,and bioinformatics was used to predict the tar?get genes that may be regulated by it. Results Compared with the control group,41 differentially expressed microRNAs were filtered in loading group,in which 20 were up?regulated and 21 were down?regulated(P values were less than 0.05 and the difference multiples were more than 1.5 times). The Quantitative Real?time PCR results showed that compared to the normal culture group,the expression level of miR?132?3p increased in loading
group,consistent with the microarray results and there was significant difference(P<0.05). After software analysis,miR?132?3p target gene may be Smad2. Conclusion The expression profile of microRNA in osteoblast is sig?nificantly affected by cyclic stretch stress,these differences may influence the biological function of osteoblast by TGF?β signaling
path?way or its related signal molecules.
【总页数】5页(P222-226)
【作者】孙芬;刘铭;刘名燕
【作者单位】山西医科大学口腔医院正畸科,山西太原030000;山西医科大学细胞生物学及遗传学教研室,山西太原030000;苏州牙博士口腔机构,江苏苏州215000【正文语种】中文
【中图分类】R783.5
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