乌司他丁对大鼠创伤性脑水肿后NF-κB及IL-1β表达的影响

乌司他丁对大鼠创伤性脑水肿后NF-κB及IL-1β表达的影响都义日;杜雪江;于建设
【期刊名称】《河北医药》
【年(卷),期】2014(000)007
【摘要】Objective To observe the changes of expression levels of nuclear factor-κB ( NF-κB) and interleukin-1β( IL-1β) in rats with traumatic encephaledema in order to explore the protective effect and possible action mechanism of ulinastatin(UTI).Methods Thirty healthy adults SD 30 rats were randomly divided into three groups:normal control group, traumatic encephaledema group and UTI intervention group .The levels of NF-κB and IL-1βin serum and brain tissue homogenate (BTH) were detected by immunohistochemisty and enzyme-linked immunosorbent assay (ELISA) at the 8th, 12th,24th,72th hour after trauma.Results The levels of NF-κB and IL-1βin encephaledema position were significantly increased,as compared with those in control group ( P <0.01),which reached peak at the 24th hour,however,which reduced slightly at the 24th hour;there were significant differences in the changes of levels of NF-κB and IL-1βin every time point between UTI gr oup and traumatic encephaledema group ( P <0.01).Conclusion UTI has obvious protective effect on the brain injury caused by traumatic encephaledema in rats , it may be one of the action mechanisms to inhibit the signal transduction way of NF-κB and IL-1β.%目的：通过观察大鼠创伤性脑水肿后脑组织中核转录
子-κB（ NF-κB）及白介素-1β（ IL-1β）的变化，探讨乌司他丁（ UTI）在大鼠
创伤性脑水肿过程中对脑组织的保护作用及其机制。

方法将30只健康成年SD大
鼠随机分为正常对照组、创伤性脑水肿组及UTI干预组，每组10只。

并于伤后8、24、72采用免疫组化法及血清ELISA法检测脑组织NF-κB和IL-1β的水平。

结
果创伤性脑水肿组和UTI组脑水肿部位NF-κB的活性和IL-1β的水平均较对照组
明显升高（ P ＜0倐．01），且于24 h达到最高值，于72 h时有所下降；UTI
组各时间点与创伤性脑水肿组比较差异均有统计学意义（ P ＜0．01）。

结论乌司他丁对大鼠创伤性脑水肿引起的脑损伤具有明显的脑保护作用，通过抑制NF-κB
和IL-1β信号传导通路是乌司他丁脑保护作用的重要机制之一。

【总页数】3页(P981-983)
【作者】都义日;杜雪江;于建设
【作者单位】010050 呼和浩特市，内蒙古医科大学附属医院麻醉科;010050 呼和浩特市，内蒙古医科大学附属医院麻醉科;010050 呼和浩特市，内蒙古医科大学
附属医院麻醉科
【正文语种】中文
【中图分类】R742
【相关文献】
1.亚低温对大鼠脑损伤后TNF-α、IL-1β及NF-κB表达的影响 [J], 王亚楠;张勇;
王宁;刘鑫颖
2.乌司他丁对急性重症胰腺炎大鼠NF-κB活性NF-κBmRNA表达的实验研究 [J], 杨超;单爱军;钟源波
3.乌司他丁对大鼠全脑缺血再灌注后NF-κB的影响 [J], 陈伟峰;刘德红;何卫平;周
丹
4.前B细胞克隆增强因子在急性呼吸窘迫综合征大鼠肺组织中表达及对炎症因子TNF-α、IL-1β、NF-κB p65表达影响的研究 [J], 刘畅;张虹;程鹏雁;周发春
5.创伤性脑水肿合并海水淹溺大鼠IL-1β和TNF-α表达的变化 [J], 齐向前;于明琨;卢亦成;胡国汉;骆纯;董艳
因版权原因，仅展示原文概要，查看原文内容请购买。