Lei Shao “The Quantitative Research On the Real Estate Bubble In China”(Google Scholar)

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小学生英语学习动机学习动力的研究课题计划

小学生英语学习动机学习动力的研究课题计划全文共3篇示例，供读者参考篇1Title: Exploring the Motivations and Driving Forces Behind English Language Learning among Elementary School Students: A Research ProposalIntroduction:In today's globalized world, the ability to communicate effectively in English has become an invaluable asset. As a language that transcends borders and cultures, English proficiency opens doors to numerous opportunities for personal growth, academic advancement, and professional success. Among the youngest learners, elementary school students, the journey towards English language acquisition begins, and it is of utmost importance to understand the motivations and driving forces that fuel their desire to learn this universal language.Research Objectives:The primary objective of this research is to explore the various factors that motivate and sustain the learning enthusiasm of elementary school students in their pursuit ofEnglish language proficiency. By identifying and analyzing these motivational elements, educators and policymakers can gain valuable insights into tailoring pedagogical approaches, curricula, and learning environments to foster a more engaging and effective English language learning experience for young learners.Specific research questions to be addressed include:What are the intrinsic and extrinsic motivations that drive elementary school students to learn English?How do factors such as parental influence, peer influence, and cultural exposure shape students' motivations for English language learning?What are the perceived benefits and future aspirations that motivate elementary school students to invest their time and effort in learning English?How do teaching methodologies, classroom environments, and extracurricular activities influence students' motivation and engagement in English language learning?What challenges or barriers do elementary school students face in maintaining their motivation for English language learning, and how can these obstacles be overcome?Research Methodology:To comprehensively investigate the motivations and driving forces behind English language learning among elementary school students, a mixed-methods approach will be employed. This approach will combine quantitative and qualitative data collection techniques to capture a holistic understanding of the research topic.Quantitative Data Collection:Survey questionnaires will be administered to a representative sample of elementary school students across multiple schools and regions. The questionnaires will include Likert-scale items and closed-ended questions to assess students' motivations, perceived benefits, and potential barriers to English language learning.Statistical analyses, such as descriptive statistics, correlations, and regression analyses, will be conducted to identify significant relationships between variables and determine the relative importance of various motivational factors.Qualitative Data Collection:Focus group discussions will be conducted with small groups of elementary school students to gain in-depth insights into theirperspectives, experiences, and personal narratives related to English language learning motivations.Semi-structured interviews will be carried out with teachers, parents, and educational experts to gather their observations and perspectives on the motivational factors influencing students' English language learning journey.Classroom observations will be conducted to examine the dynamics of teaching methodologies, classroom environments, and student engagement in English language learning activities.Data Analysis and Interpretation:The quantitative data collected through survey questionnaires will be analyzed using statistical software, such as SPSS or R. Descriptive statistics, correlation analyses, and regression models will be employed to identify patterns, relationships, and the relative importance of various motivational factors.Qualitative data from focus group discussions, interviews, and classroom observations will be transcribed and analyzed using thematic analysis techniques. Coding and categorization of recurring themes, patterns, and insights will be performed toidentify common motivational elements, challenges, and best practices.The findings from both quantitative and qualitative analyses will be triangulated and synthesized to provide a comprehensive understanding of the motivations and driving forces behind English language learning among elementary school students.Expected Outcomes and Implications:This research endeavor holds the potential to contribute valuable insights to the field of English language education, particularly in the context of elementary school students. By identifying the motivational factors that fuel young learners' enthusiasm for English language acquisition, educators and policymakers can develop targeted strategies and initiatives to nurture and sustain their learning motivation.The findings may inform the design of engaging and student-centered curricula, the implementation of effective teaching methodologies, and the creation of supportive learning environments that cater to the unique needs and motivations of elementary school students. Additionally, this research can shed light on the role of parental and cultural influences, enabling the development of collaborative approaches that involve families and communities in the English language learning process.Ultimately, by understanding and addressing the motivations and driving forces behind English language learning among elementary school students, this research aims to contribute to the overall improvement of English language education, fostering a generation of young learners who are not only proficient in English but also passionate about lifelong learning and personal growth.篇2Title: Exploring the Motivational Factors Driving English Language Learning Among Elementary School StudentsAbstract:Motivation plays a pivotal role in the language learning process, especially for young learners. This research proposal aims to investigate the intrinsic and extrinsic motivational factors that influence English language learning among elementary school students. By understanding these factors, educators and policymakers can develop effective strategies to foster a positive learning environment and enhance students' engagement in acquiring a second language.Introduction:In today's globalized world, proficiency in English has become increasingly important for personal and professional growth. Elementary school is a critical stage where students begin their journey of learning a new language, and their motivation during this period can shape their attitudes and performance in the long run. This study seeks to explore the motivational factors that drive elementary school students to learn English, including both intrinsic and extrinsic factors.Literature Review:The literature review will provide a comprehensive overview of existing research on motivation in language learning, with a specific focus on studies conducted in the context of elementary education. Key theories and models, such as theSelf-Determination Theory (Deci & Ryan, 1985) and theSocio-Educational Model (Gardner, 1985), will be examined to understand the interplay between intrinsic and extrinsic motivational factors in language learning.Research Questions:What are the intrinsic motivational factors that influence elementary school students' English language learning?What are the extrinsic motivational factors that influence elementary school students' English language learning?How do these motivational factors interact and influence students' overall engagement and performance in English language learning?Methodology:This study will employ a mixed-methods approach, combining quantitative and qualitative data collection techniques. The quantitative component will involve administering a validated questionnaire to a representative sample of elementary school students, assessing their motivational orientations, attitudes, and perceptions towards English language learning. The qualitative component will consist of focus group discussions and semi-structured interviews with students, teachers, and parents, allowing for a deeper exploration of the motivational factors and their impact on the learning experience.Data Analysis:Quantitative data will be analyzed using appropriate statistical methods, such as descriptive statistics, correlation analysis, and regression models. Qualitative data will be codedand analyzed using thematic analysis techniques to identify emerging themes and patterns related to motivational factors.Ethical Considerations:Ethical considerations will be given utmost priority throughout the research process. Informed consent will be obtained from participants, and confidentiality and anonymity will be ensured. The research will be conducted in compliance with the ethical guidelines and protocols established by the relevant institutional review boards.Expected Outcomes:The findings of this study are expected to contribute to a better understanding of the motivational factors that influence elementary school students' English language learning. By identifying the intrinsic and extrinsic motivational factors, educators and policymakers can develop targeted interventions and strategies to enhance students' motivation and create a supportive learning environment. Additionally, the study may provide insights into the interplay between different motivational factors and their impact on overall engagement and performance in language learning.篇3Title: A Study of Learning Motivation and Driving Forces in English Learning for Elementary School StudentsIntroductionAs an elementary school student, learning English can be both exciting and challenging. It's a new language with different sounds, words, and grammar rules that we have to learn from scratch. Some students seem to pick it up easily and have a strong drive to learn, while others struggle to stay motivated. I've noticed this difference in my own classroom, and it has made me curious about what factors influence a student's motivation and driving forces when it comes to learning English.The ability to communicate in English opens up a world of opportunities, from understanding movies and songs to being able to travel and connect with people from different cultures. However, the process of learning a new language can be daunting, especially for young learners. Understanding what motivates students and what drives them to persist in their English studies could help teachers and parents provide better support and create more effective learning environments.In this research proposal, I aim to explore the various motivational factors that influence elementary school students' engagement and success in learning English. By examining thesefactors, we may gain valuable insights into how to cultivate and sustain a strong learning drive among young English learners.Literature ReviewPrevious research has identified several key factors that can influence language learning motivation, including:Intrinsic motivation: This refers to the internal drive and personal enjoyment derived from learning the language itself (Dörnyei, 2001). Students who are intrinsically motivated tend to find the learning process rewarding and engaging.Extrinsic motivation: This involves external factors such as rewards, praise, grades, or parental expectations that motivate students to learn English (Ryan & Deci, 2000). While extrinsic motivators can be effective, they may not foster long-term engagement.Self-efficacy: Students' beliefs in their ability to learn and succeed in English can significantly impact their motivation and persistence (Bandura, 1997). Those with higher self-efficacy are more likely to approach challenges with confidence and perseverance.Attitude towards the target language and culture: Positive attitudes towards the English language and the culturesassociated with it can enhance motivation and interest in learning (Masgoret & Gardner, 2003).Learning environment: The classroom atmosphere, teaching methods, and support from teachers and peers can either facilitate or hinder motivation (Dörnyei & Csizér, 1998).While these factors have been explored in various contexts, there is a need for more research specifically focusing on the motivational dynamics of elementary school students learning English, as their developmental stage and learning experiences may differ from older learners.Research QuestionsTo address the gap in the literature, this study will investigate the following research questions:What are the primary motivational factors that influence elementary school students' engagement and persistence in learning English?How do intrinsic and extrinsic motivational factors interact and influence students' overall motivation?What role do self-efficacy beliefs play in shaping students' motivation and driving forces in English learning?How do attitudes towards the English language and associated cultures impact students' motivation and learning outcomes?In what ways does the learning environment (classroom atmosphere, teaching methods, peer support) affect students' motivation and driving forces?MethodologyThis study will employ a mixed-methods approach, combining quantitative and qualitative data collection methods to gain a comprehensive understanding of the research questions.Quantitative Data Collection:A survey questionnaire will be developed to assess students' motivation levels, self-efficacy beliefs, attitudes towards English and associated cultures, and perceptions of their learning environment. The questionnaire will include Likert-scale items and open-ended questions.The survey will be administered to a sample of elementary school students (grades 3-5) enrolled in English language classes in multiple schools.Qualitative Data Collection:Focus group discussions will be conducted with smaller groups of students to gain deeper insights into their motivational experiences, challenges, and driving forces.Classroom observations will be carried out to examine the learning environment, teaching practices, and student interactions that may influence motivation.Semi-structured interviews will be conducted with a subset of students, teachers, and parents to gather additional perspectives on motivational factors and strategies for enhancing learning motivation.Data AnalysisQuantitative data from the survey will be analyzed using descriptive and inferential statistics, such as correlations and regression analyses, to identify relationships between motivational factors and learning outcomes.Qualitative data from focus groups, observations, and interviews will be analyzed using thematic coding and content analysis to identify recurring themes, patterns, and insights related to the research questions.The quantitative and qualitative findings will be integrated to provide a comprehensive understanding of the motivationalfactors and driving forces influencing elementary school students' English learning.Ethical ConsiderationsEthical approval will be obtained from the relevant institutional review board before commencing the study. Informed consent will be obtained from parents/guardians and assent from student participants. Confidentiality and anonymity of participants will be maintained throughout the research process.Significance and ImplicationsThis study has the potential to contribute to the understanding of motivational factors in English language learning for elementary school students. The findings may inform teaching practices, curriculum design, and support strategies aimed at fostering and sustaining motivation among young English learners.By identifying the key motivational drivers and challenges faced by students, educators and policymakers can develop more effective interventions and create learning environments that nurture students' intrinsic motivation, self-efficacy, and positive attitudes towards English language learning.Ultimately, this research can help ensure that elementary school students develop a strong foundation and lasting motivation for acquiring English language proficiency, which can open doors to future academic, professional, and personal opportunities in an increasingly globalized world.ConclusionLearning a new language like English can be a daunting task for elementary school students, and maintaining motivation is crucial for their success. This research proposal aims to explore the various motivational factors and driving forces that influence students' engagement and persistence in English learning.By employing a mixed-methods approach and gathering data from multiple sources, this study will provide valuable insights into the interplay of intrinsic and extrinsic motivational factors, the role of self-efficacy beliefs, the impact of attitudes towards English and associated cultures, and the influence of the learning environment.The findings from this research have the potential to inform teaching practices, curriculum design, and support strategies aimed at fostering and sustaining motivation among young English learners. Ultimately, this study can contribute to creating more effective and engaging learning experiences that nurturestudents' love for language learning and set them on a path towards proficiency and future opportunities.。

3种小球藻DNA提取方法的比较

收稿　1999206216 修定　2001202226　1　国家自然科学基金(39870561)和“九五”国家科技攻关(962C02204205)资助项目。

3种小球藻D NA 提取方法的比较1陈　颖　刘根齐　李文彬　孙勇如(中国科学院遗传研究所,北京100101)Comparison of Three Extraction Methods for D NA from Chlorella spp.CHEN Y ing ,L IU G en 2Qi ,L I Wen 2Bin ,SUN Y ong 2Ru (Institute of Genetics ,The Chinese Academy of Sciences ,Beijing 100101) 提要　单细胞真核藻类小球藻已成为藻类分子生物学研究的热点,但其特殊的细胞壁组分给DNA 的提取带来了一定的困难。

文章比较了3种从小球藻中提取DNA 的方法,即纤维素酶法、作者改进的CTAB 法和目前单细胞藻类常用的裂解法。

通过所提取的DNA 进行浓度、纯度分析,认为CTAB 法是一种简单、快速和高效的适合于小球藻的DNA 提取方法。

关键词　椭圆小球藻(Chlorella ellipsoidea )　DNA 提取方法　CTAB 法作为单细胞真核藻类的小球藻因其结构简单、生长迅速、营养丰富,又可食用,目前正逐渐成为藻类分子生物学及基因工程研究的热点。

但由于其细胞壁的组分和结构较为特殊[1～4],细胞壁外层还含有可抑制多糖酶降解作用的孢粉质(sporopol 2lenin )[5],给小球藻DNA 的提取带来一定困难,从而影响了小球藻基因工程的深入研究。

要想获得高产量与高质量的DNA ,关键问题在于细胞壁的充分降解与破碎。

目前虽然已有几种小球藻藻体中DNA 的提取方法,但有些较为复杂,有些提取效果不佳[6,7]。

本文采用纤维素酶法和作者改进的CTAB 法与目前单细胞藻类常用的超声波处理法相比较,通过对提取的DNA 进行浓度、纯度分析,以期得到一种简单、快速、高效提取小球藻DNA 的方法。

quantitative and qualitative research

Mixed Methods – often combine Quantitative + Qualitative
Role of Quantitative Approaches
• To numerically define data sets • To enable any relationships between data sets to be established • To enable differences in data sets to be established • To predict a likely outcome from data sets
22
Quantitative Research Questions
3. Differences • Is there a difference in mathematics test scores before and after the implementation of a collaborative active learning strategy in a classroom ?
19
The Research Process: Schematic Outline
Plan Research
Evaluate and Research
Research Process
Do the research
Collect information
20
Research Process
• Understanding the ‘Scene’ (Literature etc) • Research Focus (Research Question, Hypothesis, Aim) • Design (Sample, Activity/Change of Practice , Data Collection, Ethics) • Implementation • Critical Analysis, Explanation & Reflection • Reporting / Sharing

有向图下的多智能体系统事件触发预设时间实用一致性

有向图下的多智能体系统事件触发预设时间实用一致性DOI ：10.19557/ki.1001-9944.2024.05.005付璐，陈霞，郭晓亚（青岛理工大学信息与控制工程学院，青岛266525）摘要：该文基于事件触发控制，研究了有向图下的多智能体系统的预设时间实用一致性问题。

首先为每个智能体提出了一个基于时变函数的事件触发控制输入，并构造合适的动态触发条件，使得系统可以在完全预设的时间内达到实用一致性。

该文提出了一个基于李雅普诺夫函数的预设时间稳定性充分条件，借助此条件给出了多智能体系统的预设时间实用一致性分析。

此外，该文从理论上严格排除了整个时间区间上的Zeno 行为。

最后，给出数值仿真实例验证了该文结果的正确性和有效性。

关键词：多智能体系统；事件触发控制；预设时间实用一致性中图分类号：TP13文献标识码：A文章编号：１００１鄄９９４４（２０24）05鄄００20鄄０6Event 鄄triggered Predefined 鄄time Practical Consensus for Multi 鄄agent Systems Under Directed GraphFU Lu ，CHEN Xia ，GUO Xiaoya（School of Information and Control Engineering ，Qingdao University of Technology ，Qingdao 266525，China ）Abstract ：In this paper ，we study the predefined 鄄time practical consensus problem for multi 鄄agent systems under di 鄄rected graphs based on event 鄄triggered control.An event 鄄triggered control input based on a time 鄄varying function is firstly proposed for each agent ，and a suitable dynamic triggering condition is constructed so that the system can achieve practical consensus at a completely predefined time.A sufficient condition for the predefined 鄄time stability based on the Lyapunov function is proposed in this paper ，with the help of which the analysis of the multi 鄄agent system predefined 鄄time practical consensus is given.In addition ，this paper rigorously excludes Zeno behavior over the whole time interval from theory.Finally ，a numerical simulation example is given to verify the correctness and validity of the results of this paper.Key words ：multi 鄄agent system ；event 鄄triggered control ；predefined 鄄time practical consensus收稿日期：2023-12-08；修订日期：2024-04-15基金项目：国家自然科学基金项目（61703225）；山东省自然科学基金项目（ZR2022MF297）作者简介：付璐（1998—），女，硕士，研究方向为多智能体系统的事件触发预设时间一致性；陈霞（通信作者）（1986—），女，博士，副教授，研究方向为事件触发控制、多智能体系统的协调控制等。

research method例子

research method例子
（最新版）
目录
1.研究方法的定义与重要性
2.研究方法的分类
3.研究方法的实际应用
4.研究方法对研究成果的影响
5.例子：使用不同的研究方法进行研究
正文
研究方法是指在进行科学研究时所采用的方法和技术，它是科学研究过程中至关重要的一环。

研究方法不仅可以帮助科学家更好地理解研究对象，还可以提高研究的准确性和可靠性，因此，选择合适的研究方法是进行科学研究的关键。

研究方法可以分为多种，包括实验法、观察法、调查法等。

实验法是通过对研究对象进行人为控制和干预，来观察和分析研究对象的反应和变化。

观察法则是通过直接观察研究对象的行为和现象，来获取研究数据。

调查法则是通过对研究对象进行问卷调查或访谈，来获取研究信息。

研究方法的实际应用取决于研究的目的和研究对象的特点。

例如，如果研究对象是人类行为，那么观察法和调查法可能更适合。

如果研究对象是自然现象，那么实验法可能更为适用。

研究方法对研究成果的影响也是不可忽视的。

不同的研究方法可能会得出不同的研究结果，因此，选择合适的研究方法可以得到更准确的研究成果。

例如，如果我们想研究一种新的药物对人体的影响，我们可以采用实验法，通过对人体进行药物注射，来观察和分析药物对人体的影响。

如果
我们想研究一种新的教育方法对学生的学习效果，我们可以采用调查法，通过对学生进行问卷调查或访谈，来获取研究信息。

nucleic acids research methods

nucleic acids research methodsNucleic acids research methods refer to the various techniques and protocols used to study and analyze nucleic acids, including DNA and RNA. These methods are employed to investigate various aspects of nucleic acids, such as their structure, function, replication, transcription, translation, and interactions with other molecules.Some commonly used nucleic acids research methods include:1. Polymerase Chain Reaction (PCR): PCR is a widely used technique for amplifying specific DNA sequences. It involves cycles of DNA denaturation, primer annealing, and DNA synthesis using a thermostable DNA polymerase enzyme.2. Gel Electrophoresis: Gel electrophoresis is a technique used to separate DNA or RNA fragments based on their size. Fragments are loaded onto an agarose or polyacrylamide gel and subjected to an electric current, causing them to migrate through the gel at different speeds.3. DNA Sequencing: DNA sequencing methods determine the nucleotide sequence of a DNA molecule. Sanger sequencing, next-generation sequencing, or third-generation sequencing techniques are commonly used for DNA sequencing.4. Northern Blotting: Northern blotting is a technique used to study gene expression by analyzing RNA samples. RNA is separated using gel electrophoresis, transferred to a membrane, and hybridized with complementary labeled probes.5. Reverse Transcription Polymerase Chain Reaction (RT-PCR): RT-PCR is used to amplify and analyze RNA sequences. It involves reverse transcribing RNA into complementary DNA (cDNA) using reverse transcriptase enzyme before amplification by PCR.6. DNA Microarray Analysis: DNA microarrays allow researchers to study gene expression patterns on a genome-wide scale. They consist of thousands of DNA probes immobilized on a solid support, and they are used to detect and quantify the expression levels of various genes simultaneously.7. Chromatin Immunoprecipitation (ChIP): ChIP is a method used to study protein-DNA interactions and protein occupancy on specific genomic regions. It involves cross-linking of proteins to DNA, chromatin fragmentation, immunoprecipitation of protein-DNA complexes, and subsequent analysis of the DNA sequences. These are just a few examples of the many methods used in nucleic acids research. Depending on the specific research goals, different combinations of techniques are employed to gain insights into the structure, function, and regulation of nucleic acids.。

Research Approach Strategies

Research Approach StrategiesResearch approach strategies are crucial for any study or investigation. These strategies help researchers to gather relevant data, analyze information, and draw meaningful conclusions. There are various research approach strategies that can be employed, each with its own strengths and weaknesses. In this response, I will discuss some of the common research approach strategies, their application, and the factors that influence their selection.One of the most widely used research approach strategies is the quantitative approach. This approach involves the collection and analysis of numerical data to understand patterns, relationships, and trends. It is often used in studies that aim to measure and quantify phenomena, such as in surveys or experiments. The quantitative approach allows for statistical analysis and generalization of findings to a larger population. However, it may not capture the depth and complexity of certain phenomena, and it may overlook qualitative aspects that are important for a comprehensive understanding.On the other hand, the qualitative approach focuses on gathering non-numerical data, such as interviews, observations, or textual analysis. This approach aims to explore the meanings, experiences, and perspectives of individuals or groups. It is particularly useful in studying complex social phenomena, such as human behavior, culture, or organizational dynamics. The qualitative approach provides rich, in-depth insights, but it may be subjective and difficult to generalize.In addition to these two main approaches, mixed methods research combines both quantitative and qualitative methods to provide a more comprehensive understanding of a research problem. This approach allows researchers to triangulate data from different sources, validate findings, and gain a more holistic perspective. However, it requires expertise in both quantitative and qualitative methods, as well as careful integration of the two types of data.The choice of research approach strategy depends on various factors, including the research question, the nature of the phenomenon under study, the available resources, and the researcher's own preferences and skills. For instance, a study aiming to test a causalrelationship between variables may benefit from a quantitative approach, while a study exploring the experiences of marginalized communities may be better suited for a qualitative approach. Researchers should also consider ethical considerations, practical constraints, and the expectations of their audience or stakeholders when selecting a research approach strategy.Moreover, the research approach strategy should be aligned with the underlying philosophical assumptions and theoretical framework of the study. Positivist researchers, who believe in an objective reality that can be measured and observed, may prefer a quantitative approach, while constructivist researchers, who emphasize the subjective construction of reality, may lean towards a qualitative approach. By grounding the research approach strategy in a coherent theoretical foundation, researchers can ensure the rigor and validity of their study.In conclusion, research approach strategies play a critical role in shaping the design and execution of a study. Whether employing a quantitative, qualitative, or mixed methods approach, researchers should carefully consider the nature of the research problem, their own expertise, and the philosophical and theoretical underpinnings of their study. By selecting an appropriate research approach strategy, researchers can enhance the validity and reliability of their findings, and contribute to the advancement of knowledge in their field.。

Quantitative Research

(Burns & Grove, as cited by Cormack, 1991, p. 140).
Marilyn K. Simon, Ph.D.
2
Characteristics of Quantitative Studies
• Quantitative research is about quantifying the relationships between variables.
– He calculated statistics showing the association between the frequency of vaccination and typhoid for each of the eleven studies, and then synthesized the statistics, thus producing statistical averages based on combining information from the separate studies.
• In causal-comparative research the independent variable is not under the researchers control, that is, the researcher can't randomly assign the participants to a gender classification (male or female) or socio-economic class, but has to take the values of the independent variable as they come. The dependent variable in a study is the outcome variable.

氯普鲁卡因通过调控lncRNA EZR-AS1表达对肺癌细胞增殖和迁移侵袭的影响

氯普鲁卡因通过调控1—RNAEZR-AS1表达对肺癌细胞增殖和迁移侵袭的影响程栋1韦玲1刘煥2【摘要】目的探索氯普鲁卡因对肺癌细胞增殖和迁移侵袭的影响及其分子机制。

方法使用i mmol/L、1.5 mmol/L、2 m m ol/L浓度的氯普鲁卡因处理肺癌A549细胞，噻唑蓝（M TT)检测细胞增殖，Tran-swell检测细胞迁移侵袭，蛋白质印迹法（Western b lo t)检测细胞周期蛋白D1 ( CyclinDl )、P21 %基质金属蛋白酶-2(M M P-2)、基质金属蛋白酶-9( MMP-9)蛋白表达，实时荧光定量PCR( qRT-PCR)检测长链非编码RNA(lncR N A)EZR反义RNA l(E Z R-A S l)表达。

在A549细胞中转染6-EZR-AS1，采用上述方法检测干扰EZR-AS1表达对细胞增殖、迁移侵袭的影响。

在A549细胞中转染P cDNA3. 1-EZR-AS1，并使用氯普鲁卡因处理细胞，观察EZR-AS1过表达在氯普鲁卡因诱导的细胞增殖、迁移侵袭中的作用。

结果不同浓度的氯普鲁卡因明显提高A549细胞增殖抑制率、P21蛋白水平，显著减少迁移细胞数、侵袭细胞数、Cyc/nD1、MMP-2、MMP-9蛋白表达量、EZR-AS1表达量，均呈浓度依赖性（A <0. 05)。

干扰IncRNA EZR-AS1表达显著增加A549细胞的抑制率、p21蛋白表达量，明显降低迁移细胞数、侵袭细胞数、Cyc/nD1、MMP-2、MMP-9蛋白水平（A <0. 05)。

lncRNAEZR-ASl过表达逆转了氯普鲁卡因对肺癌A549细胞抑制率、P21蛋白表达的促进作用，和对细胞迁移侵袭、Cyc/nD1、MMP-2、MMP-9蛋白表达的抑制作用。

结论氯普鲁卡因可能通过调控IncRNAEZR-AS1的表达，从而抑制肺癌细胞的增殖、迁移侵袭。

【关键词】氯普鲁卡因;lncRNAEZR-ASl;肺癌;增殖;迁移侵袭M fect of cloprocaine on proliferation，migration and invasion of lung cancer cells by regulating IncRNAEZR-AS1CH ENG D ong1，W EI Ling1，LIU H uan21. Department o f A nesthesiology，Dongxihu District People T H ospital，W uhan，Hubei 430040，C hina；2. Department o f A nesthesiology，Union Hospital，Tongji M edical College，Huazhong University y f Science and TecCnology，Wuh a n，Hubei 430022, China【Abstract】Objective To explore the effect of chloroprocaine on the proliferation，migration and invasion of lung cancer cells and its molecular mechanism. Methods Lung cancer A549 cells were treated with chloroprocaineat concentrations of 1mmol/L，1.5 mmol/ L，and 2 mmol/L. Cell proliferation was measured by thiazole blue(M T T)，and cell migration and invasion were detected by Transwell. The expression of cyc/n D1 ( Cyc/nD1)，P21，matrix metalloproteinase-^ ( M M P-^)，and matrix metalloproteinase-^9 ( MMP-^9) protein were determined by Westernblot，and real-time fluorescent quantitative PCR (qRT-PCR) was applied to detect long-chain non-coding RNA (Ln-cRNA) EZR-AS1 expression. A549 cells were transfected with si-EZR-AS1，and the effect of interference on EZR-AS1 expression on cell proliferation，migration，and invasion was detected using the methods describe cells were transfected with pcDNAJ. 1-EZR-AS1，and cells were treated with chloroprocaine. The role of EZR-AS1overexpression in the proliferation，migration and invasion of chloroprocaine-induced cells Different concentrations of chloroprocaine significantly increased the proliferation inhibition rate and p21 protein levelof A549 cells，and evidently reduced the number of migrating cells，invading cells，CyclinD1，MMP-!^，MM P-^9 protein expression，and EZR-AS1 expression (P <0. 05) . Interfering with the expression of lncRNAEZR-AS1 obviouslyincreased the inhibition rate and p21 protein expression of A549 cells，while dramatically reduced the number of migrating cells，invading cells，Cyc/nD1，MMP-2，and MMP-9 protein levels ( P <0. 05). Over-expression of IncRNAdoi：10.3969/j. issn. 1009 -6663.2020.12.025作者单位:1.430040湖北武汉，武汉市东西湖区人民医院麻醉科2.430022湖北武汉，华中科技大学同济医学院附属协和医院麻醉科通信作者:韦玲，E-mail:***************EZR-AS1 reversed the effect o f chloroprocaine in promoting lung cancer A549 cell inhibition rate and p21 protein expression, and inhibiting cell migration and invasion, CyclinDl #MMP-!^, and MM P-^9 protein expression. Conclusion Chlorprocaine inhibits the proliferation, migration and invasion of lung cancer cells by IncRNA EZR-AS1.【Key words】chloroprocaine; IncRNAEZR-AS1 &lung cancer; proliferation; migration and invasion肺癌是全世界最常见的恶性肿瘤之一，2018年约有210万新病例和180万死亡病例⑴。

氯碘羟喹对术后认知功能的影响

现代实用医学2020年9月第32卷第9期•1107•氯碘羟唾对术后认知功能的影响李晓瑜，翟晓杰，陈骏萍【摘要】目的观察氯碘径哇(CQ)对术后认知功能障碍(POCD)的影响并探究其相关机制。

方法40只ICR小鼠随机分为Control组、Surgery组,Clioqumol组及Saline组。

术后1d依次行旷场实验(OF)、新物体识别实验(NOR)以及条件恐惧实验(FC),并每组取6只小鼠计算海马依赖性记忆Z值,另4只小鼠取血浆与海马进行Cu2、Zrf'水平与炎症因子水平测定。

结果OF实验中，Surgery组运动能力显著低于Control组(P<0.05),Surgery组、Saline组海马依赖性记忆较Control组显著降低(均F<0.05)。

各组术后1d外周血与海马内Cu2,Zn>水平与干预前差异均无统计学意义，但Clioquinol组血浆CiAZn〉水平均低于其他3组，海马Cu>水平高于其他3组(均P<0.05).Surgery组、Saline组海马白介素-6(IL-6)和白介素-ip(IL-ip)水平均显著上调,Clioquinol组海马IL-6和IL-ip水平较Saline组显著降低(均P<0.05)。

结论CQ对术后认知具有预防性保护作用,该作用可能归咎于其对脑内Cu2、Zrf稳态的调节，也可能归咎于对中枢神经炎症的抑制,也可能两者发挥共同作用。

【关键词】认知功能障碍;铜;锌;氯碘轻唾doi:10.3969/j.issn,1671-0800.2020.09.041【中图分类号】R614【文献标志码】A【文章编号】1671-0800(2020)09-1107-03术后认知功能障碍(POCD)是一种术后常见中枢神经系统并发症，据国际POCD研究组(ISPOCD)报道，术后7d POCD发生率高达25.8%r,\POCD不仅显著降低患者工作、生活能力亦增加其死亡率，且有较大概率进展为阿尔茨海默病(AD)叫目前关于POCD的发病机制尚不清楚，有研究认为POCD的发生可能与中枢炎症反应有关，中枢内Cu21、Zn2'等金属离子稳态失衡可导致卩-淀粉样蛋白(AP)聚集和沉积,加重炎症反应,导致认知损伤％而AB的聚集沉积是导致AD的重要因素。

quantitative researcher in education解析

quantitative researcher in education解析“quantitative researcher in education”的意思是“教育领域的定量研究者”。

- “quantitative researcher”指的是采用定量研究方法进行研究的人员。

定量研究是一种基于数据和统计分析的研究方法，通常涉及收集和分析数字数据，以验证或推翻研究假设。

- “education”是“教育”的意思，这里的“education”用作定语，修饰“researcher”，表示这个研究者的研究领域是教育。

因此，“quantitative researcher in education”可以理解为在教育领域中采用定量研究方法的研究人员。

他们可能关注教育政策、教学方法、学生学习成果等方面的问题，并通过收集和分析数据来探索这些问题。

完成科研任务英语

完成科研任务英语Completing Research TasksResearch is a critical component of scientific and academic advancement. It involves systematic investigation and inquiry to expand knowledge, test hypotheses, and solve complex problems. As a researcher, the process of completing research tasks can be both challenging and rewarding. This essay will explore the key steps involved in successfully completing a research project, from defining the research question to disseminating the findings.The first and most crucial step in any research endeavor is to clearly define the research question or problem that needs to be addressed. This requires a thorough review of the existing literature to identify gaps in knowledge or areas that warrant further investigation. Researchers must also consider the relevance and significance of the proposed research, ensuring that it aligns with the broader research landscape and has the potential to contribute new insights or practical applications.Once the research question has been established, the next step is to design the research methodology. This involves selecting appropriate research methods, whether qualitative, quantitative, or a combination of both, that will enable the researcher to collect and analyze the necessary data. The research design should be well-structured, systematic, and adhere to ethical guidelines to ensure the validity and reliability of the findings.Data collection is a critical phase in the research process. Researchers may employ a variety of data collection techniques, such as surveys, interviews, experiments, observations, or secondary data analysis, depending on the nature of the research question and the available resources. Careful planning and execution of the data collection process are essential to ensure the integrity and completeness of the data.Alongside data collection, researchers must also consider the ethical implications of their work. This may involve obtaining informed consent from research participants, ensuring the confidentiality and privacy of sensitive information, and adhering to institutional review board (IRB) or ethics committee guidelines. Ethical considerations are not only a matter of professional responsibility but also crucial for maintaining the trust and credibility of the research community.Once the data has been collected, the next step is to analyze andinterpret the findings. This may involve the use of statistical software, qualitative coding techniques, or other analytical tools, depending on the research methodology. Researchers must exercise rigor and critical thinking in their analysis, identifying patterns, trends, and relationships within the data, and drawing meaningful conclusions that address the original research question.Effective communication of research findings is crucial for the dissemination of knowledge and the advancement of science. Researchers are typically expected to present their work at academic conferences, publish their findings in peer-reviewed journals, or share their insights through other channels, such as policy briefs or public presentations. The ability to communicate research effectively, both in written and oral form, is a valuable skill that researchers must cultivate.Throughout the research process, researchers must also be adept at project management and time management. Research projects often involve multiple stakeholders, deadlines, and competing demands, and researchers must be able to navigate these complexities effectively. This may involve developing a detailed project plan, setting realistic timelines, and coordinating the efforts of various team members or collaborators.In addition to technical and analytical skills, successful researchersmust also possess strong critical thinking and problem-solving abilities. Research often involves navigating unexpected challenges, setbacks, or unexpected findings, and researchers must be able to adapt their approach and think creatively to overcome these obstacles.Finally, the successful completion of a research project requires a deep commitment to continuous learning and professional development. The field of research is constantly evolving, with new methodologies, technologies, and theoretical frameworks emerging all the time. Researchers must stay informed about the latest developments in their field, engage in ongoing training and education, and be willing to embrace new approaches and ideas that have the potential to advance their work.In conclusion, completing research tasks is a multifaceted and demanding process that requires a range of skills and competencies. From defining the research question to disseminating the findings, researchers must navigate a complex landscape of ethical considerations, data collection and analysis, project management, and effective communication. By developing these skills and embracing a spirit of intellectual curiosity and perseverance, researchers can make valuable contributions to the advancement of knowledge and the betterment of society.。

科学研究的成果英语作文

科学研究的成果英语作文The breakthrough in cancer research has brought hope to millions of patients around the world. Scientists have discovered a new treatment that targets specific cancer cells, leading to significant improvements in patient outcomes.The latest findings in climate change research have revealed alarming trends in global temperatures and sea levels. It is now more urgent than ever to take immediate action to reduce carbon emissions and mitigate the impact of climate change on our planet.A recent study in neuroscience has uncovered new insights into the workings of the human brain. This discovery has the potential to revolutionize the treatment of neurological disorders and improve our understanding of the complexities of the mind.Advancements in renewable energy technology have openedup new possibilities for sustainable energy production. With the development of more efficient solar panels and wind turbines, we are one step closer to achieving a greener and more environmentally friendly future.The field of artificial intelligence continues to make significant strides, with researchers developing more sophisticated algorithms and machine learning techniques. These advancements have the potential to revolutionize various industries and improve the way we live and work.In the realm of space exploration, recent discoveries have expanded our understanding of the universe and the possibility of extraterrestrial life. With the development of new telescopes and space probes, scientists are uncovering new mysteries of the cosmos.The study of infectious diseases has led to the development of new vaccines and treatments, saving countless lives around the world. With ongoing research and collaboration, we are better equipped to combat the threat of global pandemics and prevent future outbreaks.。

合肥市周边地区乡村聚落空间结构量化研究

城市建筑城市空间 2019年3月第16卷总第313期Urbanism and Architecture17合肥市周边地区乡村聚落空间结构量化研究李浩，李早（合肥工业大学建筑与艺术学院，安徽合肥　230000）摘要：本研究主要对合肥市周边地区乡村聚落的聚居特征和空间组构进行了量化分析。

通过对多个乡村聚落的走访调研，选取了5个典型聚落样本，运用GIS 和Depthmap 软件对聚落的聚居特征和骨架结构进行分析，并结合对于聚落的实地调查，得出聚落的空间结构特征和成因，从而提出聚落更新发展中空间格局的保护及优化建议。

关键词：乡村聚落；聚居核心；空间骨架；聚落发展［中图分类号］ TU982［文献标识码］ AQuantitative Study on the Spatial Structure of Rural Settlements in the Surrounding Areas of HefeiLi Hao, Li Zao(College of Architecture and Art, Hefei University of Technology, Hefei Anhui 230000, China)Abstract: T his study mainly quantifies the characteristics and spatial fabric of rural settlements in the surrounding areas of Hefei. Through field investigation in several rural settlements, this paper selects five typical settlements samples, uses GIS and Depthmap software to analyze the settlement characteristics and frame structure of the settlement, concludes the spatial structure characteristics and the causes of the settlement, and then puts forward the protection and optimization suggestions for updating and developing the spatial pattern in the settlements.Key words: rural settlements; concentrated cores; spatial skeletons; settlement development1研究背景乡村聚落是在适应自然环境和社会文化的过程中逐渐演变而成的，其空间形态、结构、尺度、布局等都承载着聚落的文化遗存和村民的智慧。

Quantitative Research

Marilyn K. Simon, Ph.D.
10
Causal-Comparative
• Causal-comparative research attempts to establish cause-effect relationships among the variables of the study.
批注本地保存成功开通会员云端永久保存去开通
Quantitative Research
The “N” side in the Paradigm War Marilyn K. Simon, Ph.D.
Marilyn K. Simon, Ph.D.
1
QuaNtitative Paradigm
• “an inquiry into a social or human problem based on testing a theory composed of variables, measured with numbers, and analyzed with statistical procedures, in order to determine whether the predictive generalizations of the theory hold true.”
• When two variables are correlated you can use the relationship to predict the value on one variable for a participant if you know that participant’s value on the other variable.
• The attempt is to establish that values of the independent variable have a significant effect on the dependent variable.

The Need for Quantitative Imaging in Oncology：在肿瘤的定量成像的需要

The Need for Quantitative Imaging in Oncology
Richard L. Schilsky, M.D. Professor of Medicine,
Associate Dean for Clinical Research, University of Chicago
• RECIST: PD is > 20% in the sum of the longest diameters of target lesions (73% increase in volume)
• RECIST is biased toward stable disease
What is Measurable?
accepted surrogate for clinical benefit
Response Criteria
• WHO: PR is > 50% decrease in the sum of the product of the perpendicular diameters of measurable lesions
• Buyse et. al. (Lancet, 2000): meta analysis of 25 CRC trials with fluoropyrimidines: tumor response a highly significant predictor of survival, independent of PS
• Is another surrogate predictive for drugs that do not cause regression?
BAY 43-9006 12 week run-in

Lei Shao “The Quantitative Research On the Real Estate Bubble In China”(Google Scholar)

小学生英语学习动机学习动力的研究课题计划

3种小球藻DNA提取方法的比较

quantitative and qualitative research

有向图下的多智能体系统事件触发预设时间实用一致性

research method例子

nucleic acids research methods

Research Approach Strategies

Quantitative Research

氯普鲁卡因通过调控lncRNA EZR-AS1表达对肺癌细胞增殖和迁移侵袭的影响

氯碘羟喹对术后认知功能的影响

quantitative researcher in education解析

完成科研任务 英语

科学研究的成果英语作文

合肥市周边地区乡村聚落空间结构量化研究

Quantitative Research

The Need for Quantitative Imaging in Oncology：在肿瘤的定量成像的需要

完成科研任务英语