sci修改稿回答审稿人意见范文模板

sci修改稿回答审稿人意见范文模板Dear Editors and Reviewers:Thank you for your letter and for the reviewers’ comments concerning our manuscript entitled “Realtime Monitoring of Xylitol Fermentation by Micro-Raman)Spectroscopy”(LANL-2014-0001. Those comments are all valuable and very helpfulfor revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made correction which we hope meet with approval. Revised portion are marked in red in the paper. The main corrections in the paper and the responds to the reviewer’s comments are as flowing: Responds to the reviewer’s comments:1. Response to comment (Reviewer 1): (The proposed method was establishedwith the lack of the process of the optimization. The curve was adopted to illustrate the changes of absorption peak in the process of fermentation, but the absorption peak couldn’t be confirmed the identical to the reference peak of xylitol. It needs more data to prove the reliability of the method)Response: Considering the Reviewer’s suggestion, the experiment of high performance liquid chromatography (HPLC) is added to verify the reliability of the;Raman data. Raman paek is a kind of inelastic scattering paeknot absorption,peak, Raman spectrum of the standard xylitol and xylose solutions was uesd to selecte the most appropriate characteristic bands of xylitol and xylose for our experiment based on their comparative strength without overlap with other bands.2. Response to comment(Reviewer 1): (The literature data(table 1) was used inthis paper, but different shifts and strength would be obtainedusing different Raman spectrometers, so data shall be carried out according to the experimental results in the support of literature.) Response: different experimental conditions has a great influence on the peak intensity. but little effect on the peak raman shift, This is the basis of the Raman qualitative.3. Response to comment(Reviewer 1): (The references shows different styles.)Response:The format of the reference has been corrected.4. Response to comment(Reviewer 2): (The medium is a complex mixture andincludes yeast extract. Yeast extract will contain many of the characteristic Raman peaks that the authors ascribe to the yeast cells.) ;Response:every sample of yeast cells pallet had been washed twicesuspension,and centrifugationto ensure impurity elimination, in addition , yeast extract is obtained after plasmolysis and complete hydrolase autolyzed, remove the cell wall and insoluble productsaccording to the production process of yeast extract. so the process for purifing the yeast cells pallet is reasonable.5. Response to comment(Reviewer 2): ( The spectral resolution is stated to be 1.2cm-1; however, the spectra are smoothed....No details of the smoothing algorithm used are provided. This affects the spectral resolution.. )Response:we added the processing methods of Raman spectra in section of Data analysis.6. Response to comment(Reviewer 2): (In Figure 3, the peak at 866 cm-1 .....into xylulose via xylitol as intermediate." (page 7 lines 21 to 32)) Response:We try to correct the deficiencies in this passage. Wethink this is necessary to theoretically verify the Raman consistent with the fact that we know. Also ralate to the discussion on the by-products.8. Response to comment(Reviewer 3): ( It is said that inorganic salt is animportant factor for the fermentation process (Page 3, line 36), but there is no discuss or exploration about this)～Response: inorganic salt is an important factor for the fermentation processbut～the effect of inorganic salt is not the focus of the articlethe concentration ofinorganic salt is adjusted to the most suitable conditions for this strain according to the reference.9. Response to comment(Reviewer 3): (Figure 4 was used to illustrate the changesof biological macromolecules, but all the Raman peaks here were not clear)Response:Figure in the first draft of the paper has a problem in the export size by～Origin8.5and we have modified the size of resubmitted figure to ensure better view of peak.10. Response to comment(Reviewer 3): (The assignment of bands in table 1 alldepended on lectures.)Response:The Raman characteristic bands of biological macromolecular are common-sense conclusion in this field, in fact, almost all articles involved biological Raman bands of Biological macromolecule have direct quoted these conclusions. three examples were list as below : Schuster K C, Urlaub E and Gapes J R. 2000. Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and onthe heterogeneity in a culture. Journal of microbiological methods. 42(1): 29-38. Ba?ar G, K?n S. 2008. Monitoring of spectroscopic changes of a single trapped fission yeast cell by using a Raman tweezers set-up. Optics Communications. 281(19): 4998-5003.Xie C, Li Y. 2003. Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitationdifference techniques. Journal of Applied Physics. 93(5): 2982-2986.We tried our best to improve the manuscript and made some changes in the manuscript. These changes will not influence the content and framework of the paper. And here we did not list the changes but marked in revised paper.We ap preciate for Editors/Reviewers’ warm work earnestly, and hope that the correction will meet with approval. Once again, thank you very much for your comments and suggestions.YoursSincerelyZhen Huang下面是经典歌经100句,朋友经可以享受下,不需要的朋友可以下经后经经经除，，经经，，林夕经典歌经1、若只是喜经,何必夸经成经。