Preservation of eukaryotic cells using reversible

专利名称：Preservation of eukaryotic cells using reversible pore formation
发明人：Steven C. Hand,Michael A. Menze
申请号：US10965039
申请日：20041014
公开号：US07314755B2
公开日：
20080101
专利内容由知识产权出版社提供
摘要：A method is disclosed for reliably stabilizing eukaryotic cells that express the
P2Xreceptor channel, particularly mammalian and other vertebrate cells, including human cells, for example mammalian macrophages, or hematopoietic stem cells, in order to introduce otherwise membrane-impermeable compounds that are helpful for stabilizing the cells during drying, chilling, freezing, freeze-drying, or cryopreservation. The cells are exposed to extracellular ATP in concentration sufficient to open pores in the plasma membrane. One or more otherwise membrane-impermeable compounds that aid the survivorship of cells are then introduced, for example, trehalose, and after a brief time the pores are closed—for example, by adding divalent cations, or by diluting the extracellular solution. Once the trehalose or other stabilizing compound has been introduced, the cells may be stably preserved. By taking advantage of an endogenous mammalian receptor and ATP, no antigenic compounds need be introduced.
申请人：Steven C. Hand,Michael A. Menze
地址：Baton Rouge LA US,Baton Rouge LA US
国籍：US,US
代理人：John H. Runnels,Bonnie J. Davis 更多信息请下载全文后查看。