The p75NTR screening assay in order to identify mo

专利名称：The p75NTR screening assay in order to identify mojiyureta of apotoshisu
发明人：ブルインゼール，ウーター・ダビド,シク，ミロスラブ
申请号：JP2007520824
申请日：20050711
公开号：JP2008506377A
公开日：
20080306
专利内容由知识产权出版社提供
摘要： This invention to offer the method of identifying the test chemical compound ability to adjust p75NTR inductive apotoshisu, as for the said method: i.p75NTR (arrangement number 2) or with the vector which the cell dead induction fragment the cord/code is done the transfection doing the suspensoid of the eukaryotic cell, II deciding the apotoshisu response in thing, and III said cell which make chemical compound and the said cell contact which are tested (here, alteration of apotoshisu response under existing of the said test chemical compound which is compared with the apotoshisu response under absence of the said test chemical compound becomes it is index of ability of the said test chemical compound which adjusts p75NTR inductive apotoshisu) including. In this method of this invention, the suspensoid of the eukaryotic cell 0.4 - 3.0 x 104 cells/is used at the cell density of 100 l and at ratio of the transfection reagent for DNA 6 - 1, at ratio of especially 4 consists of the Hek293T cell which the transfection is done under existing of the lipid based transfection reagent. The quantity of the transfection reagent which is displayed with per last transfection mix of 10ml is in the range 8.0 - 12.0 l, and the quantity of DNA is in the range 2.0 - 3.5 g. apotoshisu
response of the cell in the method of this invention is decided making use of the method of being known in particular technical field. Especially anekishin V or nuclear dyeing is used. In desirable feature, apotoshisu response is decided anekishin V Alexa of Fluor making use 488 and Hoechst 33342. The p75NTR cell dead induction kind of fragment which is used on description above becomes, consists of, p75 Chopper domain (arrangement number 10) including, and especially p75_ICD (arrangement number 4),
p75_CD (arrangement number 6) or p75_TNF (arrangement number 8).
申请人：ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ
地址：ベルギー・ビー－２３４０－ビールセ・トウルンホウトセベーク３０
国籍：BE
代理人：小田島 平吉
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