玉米近等基因系群体的构建及其应用
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中国农业科技导报，2019, 21(12):14-22 Journal of Agricultural Science and Technology
玉米近等基因系群体的构建及其应用
李杨邹俊杰2,余佳2,徐宇2'3,徐妙云2,罗洪发"，王磊2*
(1.贵州大学农学院，贵阳550025； 2.中国农业科学院生物技术研究所，北京100081； 3.西南大学农学与合群体，利用玉米55K芯片分析了这些材料的基因型和群体结构。对2016年和2017年在廊坊与三亚 的2年4点的表型数据进行了性状差异分析及性状间相关性分析，结果表明这些材料在株型、果穗性状、籽粒 含水量等方面存在差异。利用该群体，应用全基因组关联分析准确定位到了控制穗轴颜色的基因。未来可
利用该群体结合全基因组关联分析，开展株高、穗位、籽粒脱水速率等数量性状的基因定位、分子标记开发和
U Yang12, ZOU Junjie2, YU Jia2, XU Yu2'3, XU Miaoyun2, LUO Hongfa1 * , WANG Lei"
(1 .College of Agriculture, Guizhou University, Guiyang 550025, China； 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China； 3.College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China)
Abstract: Maize (Zea mays L.) is an important food crop, and also used as an ideal material for plant genetics and functional genome research. The near-isogenic lines ( NILs) are important materials for the construction of molecular genetic maps, quantitative trait locus ( QTL) and molecular markers-assisted breeding. Maize hybrids Zhengdan 958 (Zheng 58 x Chang 7-2) and Xianyu 335 ( PH6WC x PH4CV) are widely cultivated in China, showing differences in plant architecture, ear traits, grain moisture and dehydration rate, etc. Here, Zheng 58 NILs and Chang 7-2 NILs were conducted by introducing into PH6WC and PH4CV background. These NILs were obtained by consecutively selective backcrossing and selfing, and then genotypes of these NILs were detected using maize 55 K SNP array. These NILs showed different agronomic traits including plant architecture, ear traits, grain water content and dehydration rate. And Pl locus was identified through genome-wide association study ( GWAS) analysis of cob glume color on these NILs. In the future, the NILs combined with GWAS could be used to identify genetic factors which determine plant height, ear height, grain filling rate ( GFR) and grain dehydration rate ( GDR). And SNP molecular markers would be developed for molecular marker-assisted selection breeding to improve breeding efficiency. Key words:near-isogenic lines； SNP array； genome-wide association study； cob color； molecular marker
摘要:玉米是我国重要的粮食作物,也是研究植物遗传和功能基因组学的理想材料。近等基因系群体是构
建分子遗传图谱、QTL定位和分子辅助育种重要的材料。我国广泛栽培的杂交种先玉335和郑单958在植株
形态、果穗性状、籽粒含水量和脱水速率等方面存在差异。以郑单958亲本郑58和昌7-2为材料，分别与先
玉335的亲本PH6WC和PH4CV进行杂交、回交和多代自交，构建了含有先玉335亲本背景的近等基因系材
分子辅助育种等工作，提高育种效率。
关键词:近等基因系;SNP芯片;全基因组关联分析;穗轴颜色;分子标记
doi: 10.13304/j.nykjdb.2019.0136
中图分类号:Q78
文献标识码：A
文章编号：1008-0864(2019)12-0014-09
Construction of Maize Near-Isogenic Lines and Its Application
玉米近等基因系群体的构建及其应用
李杨邹俊杰2,余佳2,徐宇2'3,徐妙云2,罗洪发"，王磊2*
(1.贵州大学农学院，贵阳550025； 2.中国农业科学院生物技术研究所，北京100081； 3.西南大学农学与合群体，利用玉米55K芯片分析了这些材料的基因型和群体结构。对2016年和2017年在廊坊与三亚 的2年4点的表型数据进行了性状差异分析及性状间相关性分析，结果表明这些材料在株型、果穗性状、籽粒 含水量等方面存在差异。利用该群体，应用全基因组关联分析准确定位到了控制穗轴颜色的基因。未来可
利用该群体结合全基因组关联分析，开展株高、穗位、籽粒脱水速率等数量性状的基因定位、分子标记开发和
U Yang12, ZOU Junjie2, YU Jia2, XU Yu2'3, XU Miaoyun2, LUO Hongfa1 * , WANG Lei"
(1 .College of Agriculture, Guizhou University, Guiyang 550025, China； 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China； 3.College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China)
Abstract: Maize (Zea mays L.) is an important food crop, and also used as an ideal material for plant genetics and functional genome research. The near-isogenic lines ( NILs) are important materials for the construction of molecular genetic maps, quantitative trait locus ( QTL) and molecular markers-assisted breeding. Maize hybrids Zhengdan 958 (Zheng 58 x Chang 7-2) and Xianyu 335 ( PH6WC x PH4CV) are widely cultivated in China, showing differences in plant architecture, ear traits, grain moisture and dehydration rate, etc. Here, Zheng 58 NILs and Chang 7-2 NILs were conducted by introducing into PH6WC and PH4CV background. These NILs were obtained by consecutively selective backcrossing and selfing, and then genotypes of these NILs were detected using maize 55 K SNP array. These NILs showed different agronomic traits including plant architecture, ear traits, grain water content and dehydration rate. And Pl locus was identified through genome-wide association study ( GWAS) analysis of cob glume color on these NILs. In the future, the NILs combined with GWAS could be used to identify genetic factors which determine plant height, ear height, grain filling rate ( GFR) and grain dehydration rate ( GDR). And SNP molecular markers would be developed for molecular marker-assisted selection breeding to improve breeding efficiency. Key words:near-isogenic lines； SNP array； genome-wide association study； cob color； molecular marker
摘要:玉米是我国重要的粮食作物,也是研究植物遗传和功能基因组学的理想材料。近等基因系群体是构
建分子遗传图谱、QTL定位和分子辅助育种重要的材料。我国广泛栽培的杂交种先玉335和郑单958在植株
形态、果穗性状、籽粒含水量和脱水速率等方面存在差异。以郑单958亲本郑58和昌7-2为材料，分别与先
玉335的亲本PH6WC和PH4CV进行杂交、回交和多代自交，构建了含有先玉335亲本背景的近等基因系材
分子辅助育种等工作，提高育种效率。
关键词:近等基因系;SNP芯片;全基因组关联分析;穗轴颜色;分子标记
doi: 10.13304/j.nykjdb.2019.0136
中图分类号:Q78
文献标识码：A
文章编号：1008-0864(2019)12-0014-09
Construction of Maize Near-Isogenic Lines and Its Application