METHOD FOR CONSTRUCTING FULL-LENGTH cDNA LIBRARIES

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专利名称:METHOD FOR CONSTRUCTING FULL-LENGTH cDNA LIBRARIES

发明人:OTA, Toshio, Kyowa Hakko Kogyo

Co.,Ltd.,WAKAMATSU, Ai,ISOGAI,

Takao,OTSUKA, Kaoru,SUZUKI,

Yutaka,SUGANO, Sumio

申请号:EP00944309.4

申请日:20000706

公开号:EP1195434A1

公开日:

20020410

专利内容由知识产权出版社提供

摘要:The present inventors have developed methods that enable the construction of cDNA libraries with extremely high fullness ratio from a very small amount of starting as compared with conventional methods by optimizing reaction conditions in the steps of the alkaline phosphatase reaction, acid pyrophosphatase reaction, and RNA ligase reaction of conventional oligo-capping methods.

申请人:Helix Research Institute

地址:1532-3 Yana Kisarazu-shi, Chiba 292-0812 JP

国籍:JP

代理机构:VOSSIUS & PARTNER

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