糖尿病结肠动力障碍大鼠远端结肠平滑肌细胞bax、bcl-2及caspase-3的表达

糖尿病结肠动力障碍大鼠远端结肠平滑肌细胞bax、bcl-2及
caspase-3的表达
孙曼怡;刘燕;冯凭
【期刊名称】《郑州大学学报（医学版）》
【年(卷),期】2012(047)003
【摘要】目的:探讨糖尿病(DM)结肠动力障碍大鼠远端结肠平滑肌细胞凋亡基因的表达.方法:雄性SD大鼠18只应用链脲佐菌素40 mg/kg尾静脉注射后成功建立DM结肠动力障碍大鼠模型,分为模型 6周和10周组;另18只分为正常对照6周和10周组,各组9只大鼠.应用实时荧光定量PCR检测各组大鼠远端结肠平滑肌细胞的bax、bcl-2和caspase-3 mRNA表达,采用免疫组化SP法检测其蛋白的表达.结果:不同组别和作用时间各组大鼠远端结肠平滑肌细胞bax、bcl-2、caspase-3 mRNA和蛋白的表达差异均有统计学意义(P均<0.001),模型组与同一时间点正常对照组以及模型10周组与6周组大鼠比较,远端结肠平滑肌细胞bax 和 caspase-3 mRNA和蛋白的表达增强,bcl-2 mRNA和蛋白的表达降低
(P<0.05).结论:远端结肠平滑肌细胞凋亡基因bax、bcl-2及caspase-3表达的改变可能是DM大鼠结肠动力障碍发病机制之一.%Aim:To explore the gene expressions of apoptosis in distal colon smooth muscle cells in diabetes mellitus colon dysmotility rats.Methods:A total of 36 male SD rats were randomly divided into 4 groups which included normal control 6 weeks group,normal control 10 weeks group, model 6 weeks group and model 10 weeks group,9 rats in each group.Diabetes mellitus colon dysmotility was induced by streptozotocin(40 mg/kg).The expressions of bax,bcl-2 and
caspase-3 mRNA in the distal colon smooth muscle cells were determined by real-time quantitative PCR. Immunohistochemistry SP method was used to detect the expressions of bcl-2,bax and caspase-2 proteins.Results:The expressions of bax and caspase-3 mRNA and protein in model groups were higher than those in the normal control groups and the expressions of bcl-2 mRNA and protein in model groups were lower than those in the normal control groups(P<0.05). The expressions of bax and caspase-3 mRNA and protein in model 10 weeks group were higher than those in model 6 weeks group and the expressions of bcl-2 mRNA and protein in model 10 week group were lower than those in model 6 week
group(P<0.05).Conclusion:The expressions of bax,caspase-3,and bcl-2 in distal colon smooth muscle cell may contribute to the pathogenesis of diabetic colon dysmotility.
【总页数】4页(P344-347)
【作者】孙曼怡;刘燕;冯凭
【作者单位】天津医科大学总医院代谢病科,天津,300052;天津医科大学总医院代谢病科,天津,300052;天津医科大学总医院代谢病科,天津,300052
【正文语种】中文
【中图分类】R58
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