USP关于色谱柱填料的规定

USP关于色谱柱填料的规定
我们在实验中常常用到USP作为检验依据，其中有一些关于色谱柱的要求现将其中个色谱柱要求罗列如有不恰当的请大家指正L1和L8是美国药典(USP)规定的色谱柱编号，其实就是C18柱和NH2柱。

下面是对应的色谱柱类型。

L1：十八烷基键合多孔硅胶或无机氧化物微粒固定相，简称C18或ODS
L2：30～50um表面多孔薄壳型键合C18(ODS)固定相
L3：多孔硅胶微粒即一般的硅胶柱
L4：30～50um表面多孔薄壳型硅胶
L5：30～50um表面多孔薄壳型氧化铝
L6：30～50um实心微球表面包覆磺化碳氟聚合物－强阳离子交换固定相
L7：全多孔硅胶微粒键合C8官能团固定相简称C8柱
L8：全多孔硅胶微粒键合非交联NH2固定相简称NH2柱
L9：强酸性阳离子交换基团键合全多孔不规则形硅胶固定相
L10：多孔硅胶微球键合氰基固定相（CN）简称CN柱
L11：键合苯基多孔硅胶微球固定相简称苯基柱
L12：无孔微球键合季胺功能团的强阴离子填料
L13：三乙基硅烷化学键合全多孔硅胶微球固定相（C1）简称C1柱
L14:10um硅胶化学键合强碱性季铵盐阴离子交换固定相简称SAX柱
L15：已基硅烷化学键合全多孔硅胶微球固定相简称C6柱
L16：二甲基硅烷化学键合全多孔硅胶微粒固定相
L17：氢型磺化交联苯乙烯-二乙烯基苯共聚物,强阳离子交换树脂L18: 3～10um全多孔硅胶化学键合胺基（NH2）和氰基（CN）L19：钙型磺化交联苯乙烯－二乙烯基苯共聚物，强阳离子交换树脂
L20：二羟基丙烷基化学键合多孔硅胶微球固定相（Diol）简称二醇基柱
L21：刚性苯乙烯－二乙烯基苯共聚物微球
L22：带有磺酸基团的多孔苯乙烯阳离子交换树脂
L23：带有季胺基团的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔离子交换树脂
L24：表面含有大量羟基的半刚性聚乙烯醇亲水凝胶
L25：聚甲基丙烯酸酯树脂交联羟基醚（表面含有残余羧基功能团）树脂。

能分离分子量100～5000MW范围的水溶性中性、阳离子型及阴离子型聚合物（用聚氧乙烯测定）的固定相
L26：丁基硅烷化学键合全多孔硅胶微球固定相
L27：30～50um的全多孔硅胶微粒
L28：多功能载体，100?的高纯硅胶加以氨基键合以及C8反相键合的官能团
L29: 氧化铝,反相键合,含碳量低,氧化铝基聚丁二稀小球,5um，孔径80?
L30: 全多孔硅胶键合乙基硅烷固定相
L31: 季胺基改性孔径2000?的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂
L32: L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料L33: 能够分离分子量4000～40000MW范围蛋白质分子的球形硅胶固定相,pH稳定性好L34：铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9um球形
L35：锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150?
L36: 5um胺丙基硅胶键合L-苯基氨基乙酸－3,5二硝基苯甲酰
L37：适合分离分子量2000～40,000Mw的聚甲基丙烯酸酯凝胶
L38：水溶性甲基丙烯酸酯基质SEC色谱柱
L39：亲水全多孔聚羟基甲基丙烯酸酯色谱柱
L40：Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球
L41：球形硅胶表面固定α1酸糖蛋白固定相
L42: C8和C18硅烷化学键合多孔硅胶固定相
L43: 硅胶微球键合五氟代苯基固定相
L44: 多功能固定相,60 ?高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团L45: β-环糊精键合多孔硅胶微球
L46: 季胺基改性苯乙烯-二乙烯基苯聚合物微球
液相色谱柱
USP column Packing
L1 —— Octadecyl silane chemically bonded to porous silica or ceramic micro-particles,3 to 10um in diameter
L2 —— Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50um in diameter.
L3 —— Porous silica particles, 5 to 10um in diameter.
L4 —— Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50um in diameter.
L5 —— Alumina of controlled surface porosity bonded to a solid spherical core , 30 to 50um in diameter.
L6 ——Strong cation –exchange packing-sulfonated fluorocarbon polymer coated on a solid spherical, 30 to 50um in diameter.
L7 —— Octylsilane chemically bonded to totally porous silica particles , 3 to 10um in diameter.
L8 ——An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 10um in diameter.
L9 ——10um irregular or spherical layer of aminopropylsilane chemically bonded, strongly acidic cation-exchange coating.
L10- Nitrile groups chemically bonded to porous silica
particles, 3 to 10um in diameter.
L11- Phenyl group chemically bonded to porous silica particles, 5 to 10um in diameter.
L12- A strong anion-exchange packing made by chemically bonding a quaternary ammonium anion-exchange coating.
L13- Trimethylsilane chemically bonded to porous silica particles, 3 to 10um in diameter.
L14 –Silica gel 10um in diameter having a chemically bonding a quaternary ammonium anion-exchange coating.
L15 – Hexylsilane chemically bonded to totally porous silica particles, 3 to 10um in diameter.
L16 –Dimethylsilane chemically bonded to porous silica particles, 5 to 10um in diameter.
L17_ Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen from, 7 to 11um in diameter.
L18 – Amion and cyano groups chemically bonded to porous silica particles, 3 to 10um in diameter.
L19 – Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divilbenzene copolymer in the calcaium from, about 10um in diameter.
L20- Dihydroxypropane groups chemically bonded porous silica particles , 5 to 10um in diameter.
L21 – A rigid, spherical styrene-divinylbenxene copolymer, 5 to
10um in diameter.
L22 – A cation-exchange resin made of porous polystyrene gel with sulfonic acid group, about 10um in diameter.
L23 –An cation-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium
groups, about 10um in size.
L24 — A semi-right hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface , 32 to 63um in diameter.5
L25 _Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with
poly-hydroxylated ether (surface contained some residual carboxylfunctional groups) was fund suitable.
L26- Butyl silica chemically bonded to totally porous silica particles, 5 to 10um in diameter.
L27 _ Porous silica particles, 30 to 5um in diameter.
L28 –A multifunctional support, which consists of a high purity, 100 ?, spherical silica substrate that has been bonded with anionic exchange, amine functionality in addition to a conventional reversed phase C8 functionality.。