输血科冰箱空气培养流程

输血科冰箱空气培养流程
English Answer:
Blood Bank Refrigerator Air Culture Procedure.
Purpose:
To monitor the air quality within the blood bank refrigerator for the presence of microorganisms that may impact the safety and integrity of stored blood components.
Materials:
Sterile blood culture bottles with tryptic soy broth.
Incubator.
Alcohol prep pads.
Gloves.
Safety glasses.
Procedure:
1. Prepare the refrigerator:
Turn off the refrigerator and disconnect it from the power source.
Remove all blood components from the refrigerator.
Clean the interior of the refrigerator with an appropriate disinfectant.
2. Place culture bottles:
Open one blood culture bottle and remove the rubber stopper.
Place the open culture bottle in the center of each shelf in the refrigerator.
Replace the rubber stopper.
3. Incubate:
Turn on the incubator and set the temperature to 37°C.
Place the culture bottles in the incubator for 7 days.
4. Observe:
Incubate the culture bottles for 7 days. Observe the bottles daily for any signs of microbial growth, such as cloudiness, turbidity, or gas production.
5. Interpret results:
No growth: The air quality in the refrigerator meets acceptable standards.
Growth observed: Investigate the source of the contamination and take appropriate corrective actions.
Maintenance:
Perform air cultures on a regular basis, as determined by the quality control program.
Replace the culture bottles every 7 days.
Reporting:
Document the results of the air cultures.
If any contamination is detected, report it to the appropriate personnel and initiate corrective actions.
中文回答：
输血科冰箱空气培养流程。

目的：
监测输血科冰箱的空气质量，检查可能影响储存血液成分的安
全性和完整性的微生物。

材料：
含有胰蛋白胨肉汤的无菌血培养瓶。

培养箱。

酒精棉球。

手套。

护目镜。

程序：
1. 准备冰箱：
关闭冰箱并将其与电源断开。

从冰箱中取出所有血液成分。

用适当的消毒剂清洁冰箱内部。

2. 放置培养瓶：
打开一个血培养瓶并取下橡胶塞。

将开放的培养瓶放在冰箱每个搁板的中央。

更换橡胶塞。

3. 培养：
打开培养箱并将温度设定为37°C。

将培养瓶置于培养箱中孵育 7 天。

4. 观察：
将培养瓶培养 7 天。

每天观察培养瓶是否有任何微生物生长的迹象，例如浑浊、混浊或产气。

5. 解释结果：
未见生长，冰箱内的空气质量符合可接受的标准。

观察到生长，调查污染源并采取适当的纠正措施。

维护：
根据质量控制计划定期进行空气培养。

每 7 天更换培养瓶。

报告：
记录空气培养的结果。

如果发现任何污染，请向相关人员报告并采取纠正措施。