RP-HPLC法测定人血浆中替考拉宁的浓度

RP-HPLC法测定人血浆中替考拉宁的浓度
江泓颖;曹薇;陈永刚;吴金虎;王雄
【期刊名称】《广东药学院学报》
【年(卷),期】2011(27)5
【摘要】目的建立测定人血浆替考拉宁的HPLC法,供血药浓度监测用,指导临床安全合理用药.方法色谱柱为Wondasil C18柱(4.6 mm ×250 mm,5μm)；流动相为乙腈-0.05 mol/L KH2 PO4溶液(体积比28∶72,pH=3.2),检测波长为240 nm,流速为1.0 mL/min；柱温为25℃.结果替考拉宁在4～48 μg/mL质量浓度范围内线性关系良好,检测限为2 μg/mL,方法回收率平均值为91.43％,提取回收率平均值为88.40％,日内、日间RSD值均小于6％.结论该方法操作简单快速,准确度高,适用于替考拉宁的血药浓度测定.%Objective To establish an HPLC method for determination of teicoplanin in serum, providing the basis for monitoring clinical plasma concentrations and rational administration. Methods HPLC was performed on a C18 column (Wondasil,4. 6 mm × 250 mm,5μm) with acetonitrile-0.05 mol/L monobasic potassium phosphate 28:72 as mobile phase. The wavelength was 240 run, and the flow rate was 1. 0 mL/min, colu mn temperature was 25℃. Results It showed good linearity in the concentration range of 4 -48 μg/mL,and the limit of quantitation was 2 μg/mL, The average method recovery was 91. 43% and the average extraction recovery was 88.40%. The inter-day RSD and intra-day RSD were both less than 6%. Conclusion The method was simple,quick and accurate,which could be used for the assay of teicoplanin in serum.
【总页数】3页(P496-498)
【作者】江泓颖;曹薇;陈永刚;吴金虎;王雄
【作者单位】武汉市第三医院,湖北武汉430060;武汉市第三医院,湖北武汉430060;武汉市第三医院,湖北武汉430060;武汉市第三医院,湖北武汉430060;武汉市第三医院,湖北武汉430060
【正文语种】中文
【中图分类】R927.2
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