DETECTION AND ANALYSIS OF METHYLATION IN NUCLEIC A
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专利名称：DETECTION AND ANALYSIS OF
METHYLATION IN NUCLEIC ACID
SEQUENCES
发明人：DREW, HORACE R.,MOLLOY, PETER LAURENCE,BROWN, GLENN S.
申请号：EP10807798
申请日：20100812
公开号：EP2464748A4
公开日：
20121205
专利内容由知识产权出版社提供
摘要：Provided herein are methods for the identification of methylated cytosine bases in a nucleotide sequence, the method comprising the steps of: (a) generating fragments of a DNA molecule comprising the nucleotide sequence such that the fragments comprise at least one single-stranded terminus comprising one or more bases and wherein at least one base within the single-stranded terminus is an unmethylated cytosine or methylcytosine; (b) incubating the fragments with bisulfite under non-denaturing conditions such that only unmethylated cytosines within the at least one single- stranded terminus are converted to uracil; and (c) detecting fragments comprising methylcytosine or uracil bases in the at least one single-stranded terminus. Also provided are methods for the diagnosis of, or determination of susceptibility to, diseases associated with aberrant cytosine methylation.
申请人：COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION 更多信息请下载全文后查看。