TMT-18-6006中文资料
BASF (China) Company Ltd. Shanghai Branch 巴斯夫
Plastics Division Fax
Engineering Plastics Bob Chen Patrick Miao Micheal Sun ASnagberinlaaShen Liang PPaoulyl uXrieethan e GFleoorergneceZhao Wang Derek Wang Jasmine Xi Patrick Zhao July Xu Bill Lu HDeinngryJiGanu Jiang JSatymreSnhici Polymers Alex Zhang CR.oYla.Pndark Huang Jessie Du PReBtMer Sun Polyurethan Jay Yang Shelby Yu Ge Hao Yong
Ext
6999 3000
6499 6407 6405 6475 6404
6488 6700 6704 6705 6706
6199 6101 6701 6710 6711 6709
6011 6012 6013 6014
SPheetrrowcinheXmieic als Dou Zheng Karen Wang Jim Gu IFviybeYrang Intermediate GFrearnryceSsong Jiang Phoebe Xu Elaine Zhou
BASF (China)
Switch Board
General Fax:
Management Fax Johnny Kwan
Rena Gui Finance and Corporate Services Fax Henry Choo Melody Zhang Finance , Accounting & Fax Anita Wang Accounting Kitty Shan Linda Lin Jimmy Xu Sherry Sun Catherine Qian Jie Ming Liu Min Finance Fax Lynda Xu Jennifer Wang Controlling Laura Wang Logistics and Supply Chain Fax
iec60068标准名称
iec60068标准名称IEC 60068 是国际电工委员会（International Electrotechnical Commission，IEC）发布的一系列标准，涵盖了环境试验的各个方面。
IEC 60068 标准系列主要用于规定电子和电气设备在各种环境条件下的测试方法，以验证其耐久性和稳定性。
IEC 60068 标准的全称是"IEC 60068-<部分编号>"，其中"<部分编号>" 代表具体的部分或子标准。
每个部分通常涵盖一种特定类型的环境试验。
以下是一些IEC 60068 标准的示例：1.IEC 60068-2-1: Environmental testing - Part 2-1: Tests -Tests A: Cold•环境试验- 第2-1部分：试验- 试验A：低温2.IEC 60068-2-2: Environmental testing - Part 2-2: Tests -Tests B: Dry heat•环境试验- 第2-2部分：试验- 试验B：干热3.IEC 60068-2-6: Environmental testing - Part 2-6: Tests - TestFc: Vibration (sinusoidal)•环境试验- 第2-6部分：试验- 试验Fc：振动（正弦）4.IEC 60068-2-27: Environmental testing - Part 2-27: Tests -Test Ea and guidance: Shock•环境试验- 第2-27部分：试验- 试验Ea及指导：冲击这些标准覆盖了温度、湿度、振动、冲击等多个环境因素，旨在确保电子和电气设备在各种极端条件下仍能正常工作。
具体的IEC 60068 标准名称可能因部分编号而有所不同。
如果需要查找特定的标准，建议参考IEC 的官方文档或相关出版物。
TMT国际贸易实训,上海金海贸易公司,操作五
金海贸易公司GOLDEN SEA TRADING CORPORATIONADD.: 8TH FLOOR, JIN DU BUILDING ,TEL: 86-21-64331255277 WU XING ROAD,FAX: 86-21-64331256SHANGHAI, CHINATO: F.L.SMIDTH & CO. A/S FAX: （01）20 11 90DATE: March 22, 2003Dear Sirs,We have carefully considered the opinion you expressed in your mail of counter offer. We are doing the best to set our price as low as possible without a sacrifice of quality in searching the suitable suppliers. Though we may possibly accept your payment term, i.e. by L/C at 30 days' sight, we have regretfully point out that the prices mentioned in your mail are unacceptable.Considering the excellent quality submitted and the continual rise in export cost, it is almost impossible for us to make any further reduction. However, in view of the initial transaction between us and the special character of your market, we have decided to give you the following favorable quotation, which is the utmost we can do:FOREVER BRAND BICYCLE:YE80326'USD66.00 per set CIFC5 CopenhagenTE60024'USD71.00 per set CIFC5 CopenhagenSince this offer is valid only for 3 days, please take this advantage and give us your acceptance by E - mail as soon as possible.With best regards!Yours faithfully,GOLDEN SEA TRADING CORP.Manager XXX。
iec60068标准中文版
iec60068标准中文版
这一部分主要规定了各种环境条件下进行测试时的一般要求，例如温度、湿度、气压、震动和冲击等指标的基本要求和标准测试方法。
这一部分详细描述了测试设备在各种环境条件下的工作状态和性能要求。
包括温度试验、湿度试验、水试验、振动试验和冲击试验等等。
这些
试验方法旨在模拟设备在实际使用中可能遇到的各种环境条件，以评估设
备的可靠性和适应性。
这一部分重点介绍了试验项的选择和特殊试验方法，用于评估设备在
特定环境条件下的性能。
例如，该标准规定了设备在高温、低温、高湿度、低湿度、海洋气候和太阳辐射等特殊环境条件下的测试方法。
这一部分提供了一系列特殊环境条件下的试验方法，包括试验设备在
恒温和循环温度条件下的性能测试、设备在高湿度条件下的工作性能测试、设备在高低温交变条件下的工作性能测试等。
TMT 蛋白标记试剂盒使用说明90064
INSTRUCTIONSTMT Mass Tagging Kits and90060 TMTduplex Isotopic Label Reagent Set, sufficient reagents for 5 duplex isotopic experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mg90061 TMTsixplex Isobaric Label Reagent Set, sufficient reagents for 1 sixplex isobaric experiment Contents:TMT6-126 Label Reagent, 1 × 0.8mgTMT6-127 Label Reagent, 1 × 0.8mgTMT6-128 Label Reagent, 1 × 0.8mgTMT6-129 Label Reagent, 1 × 0.8mgTMT6-130 Label Reagent, 1 × 0.8mgTMT6-131 Label Reagent, 1 × 0.8mg90062 TMTsixplex Isobaric Label Reagent Set, sufficient reagents for 2 sixplex isobaric experiments Contents:TMT6-126 Label Reagent, 2 × 0.8mgTMT6-127 Label Reagent, 2 × 0.8mgTMT6-128 Label Reagent, 2 × 0.8mgTMT6-129 Label Reagent, 2 × 0.8mgTMT6-130 Label Reagent, 2 × 0.8mgTMT6-131 Label Reagent, 2 × 0.8mg90063TMTduplex Isobaric Mass Tagging Kit, sufficient reagents for 5 duplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mgDissolution Buffer (1 M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLPierce™Trypsin Protease, MS Grade, 2 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90064TMTsixplex Isobaric Mass Tagging Kit, sufficient reagents for 5 sixplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mgDissolution Buffer (1M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5 M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLPierce Trypsin Protease, MS Grade, 5 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90065TMTduplex Isobaric Label Reagent Set, sufficient reagents for 5 duplex isobaric experiments Contents:TMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mg90066TMTsixplex Label Reagent Set, sufficient reagents for 5 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mg90067TMTzero Label Reagent, 5 × 0.8mg, sufficient reagents for 5 samples90068TMTsixplex Label Reagent Set, sufficient reagents for 12 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 2 × 5mgTMT6-127 Label Reagent, 2 × 5mgTMT6-128 Label Reagent, 2 × 5mgTMT6-129 Label Reagent, 2 × 5mgTMT6-130 Label Reagent, 2 × 5mgTMT6-131 Label Reagent, 2 × 5mgStorage: Upon receipt store at -20°C. Reagents are shipped with dry ice.Note: These products are for research use only − do not use for diagnostic procedures.ContentsIntroduction (3)Procedure Summary (4)Important Product Information (4)Additional Materials Required (4)Material Preparation (5)Preparing and Labeling Peptides with the TMT Isobaric Mass Tags (5)Troubleshooting (6)Additional Information (6)A.Data Acquisition Methods (6)B.Data Analysis and Quantitation (7)rmation Available from our Website (8)Related Thermo Scientific Products (8)General References (8)IntroductionThe Thermo Scientific™ TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex relative quantitation by mass spectrometry (MS). Each mass-tagging reagent within a set has the same nominal mass (i.e., isobaric) and chemical structure composed of an amine-reactive NHS-ester group, a spacer arm and an MS/MS reporter (Figure 1). The reagent sets can be used to label two or six peptide samples prepared from cells or tissues. For each sample, a unique reporter in the low mass region of the MS/MS spectrum (i.e., 126-127Da for TMT2 and 126-131Da for TMT6 Isobaric Label Reagents) is used to measure relative protein expression levels during peptide fragmentation.The TMTduplex™ Isotopic Label Reagent Set contains TMTzero™ and one of the TMTsixplex™ Reagents (TMT6-127) to be used as “light” and “heavy” tags for MS-level peptide quantitation similar to duplex isotopic metabolic labeling (e.g., SILAC) or isotopic dimethylation labeling. These isotopic pairs can also be used in targeted quantitation strategies, including selective reaction monitoring (SRM, see the Additional Information Section). Advantages of the TMTduplex and TMTsixplex Isobaric Label Reagents include increased sample multiplexing for relative quantitation, increased sample throughput and fewer missing quantitative channels among samples.Figure 1.Chemical structure of the TMTLabel Reagents. A. Functional regions of thereagent structure, including MS/MSfragmentation sites by higher energy collisiondissociation (HCD) and electron transferdissociation (ETD). B. TMTduplex Reagentstructures and isotope positions (*); only HCDdifferentiates between these two reporters.C. TMTsixplex Reagent structures and isotopepositions (*).Procedure SummaryProtein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean-up. Labeled samples are analyzed by high resolutionOrbitrap LC-MS/MS before data analysis to identify peptides and quantify reporter ion relative abundance (Figure 2).Figure 2. Schematic for using the Thermo Scientific TMTsixplex Isobaric Mass Tagging Reagents.Important Product Information• The TMT Reagents are moisture-sensitive. To avoid moisture condensation onto the product, vial must be equilibrated to room temperature before opening.•Anhydrous acetonitrile is the recommended solvent to dissolve reagents. Stock solutions are stable for one week when stored at -20°C. For long term storage of unused reagent, remove all solvent by drying and store with desiccant at -20°C. Anhydrous ethanol can be used as an alternative solvent to dissolve reagents but is not recommended for stock solution storage.• The TMT Reagents are amine-reactive and modify lysine residues and the peptide N-termini. All amine-containing buffers and additives must be removed before digestion and labeling.• All samples must be digested, labeled and then mixed equally before desalting, fractionation and LC-MS/MS. For optimal results, use 25-100µg of peptide for each labeling reaction.• To avoid contamination of MS samples, always wear gloves when handling samples and gels. Use ultrapure MS-grade reagents. Perform sample preparation in a cleaned work area.• The TMTzero Label Reagent can be used to optimize methods before multiplexed analysis of samples with the TMTduplex or TMTsixplex Reagent Set.Additional Materials Required• Microcentrifuge tubes• Anhydrous acetonitrile (Thermo Scientific™ Acetonitrile HPLC grade, Product No. 51101) • Water, LC-MS Grade (Product No. 51140) • Chilled (-20°C) acetone• Protein assay (e.g., Thermo Scientific™ BCA Protein Assay Kit, Product No. 22235) • 75-300µm capillary C 18 reversed-phase column• High-resolution Orbitrap Mass Spectrometer, ion trap or time-of-flight (TOF) mass spectrometer with online or offline liquid chromatography (LC) system• Data analysis software such as Thermo Scientific™ Proteome Discoverer™ or Mascot™ Software (Matrix Science, Ltd.)• Optional: C18 spin tips or columns (e.g., Thermo Scientific™ Pierce™ C18 Spin Columns, Product No. 89870 or Pierce™ C18 Tips, Product No. 87784)Material PreparationNote: The 50% hydroxylamine and 10% SDS stock solutions provided with the kit may precipitate during storage. Warm both solutions to room temperature and vortex before use. The amounts listed below are sufficient for preparing and labeling 6 samples.Add 500µL of the Dissolution Buffer (1M TEAB) to 4.5mL of ultrapure water.100mM TEAB (triethylammonium bicarbonate)Lysis Buffer Add 200µL of the Denaturing Reagent (10% SDS) to 1.8mL of 100mM TEAB.200mM TCEP Add 70µL of the Reducing Reagent (0.5M TCEP) to 70µL of ultrapure water. Then add 35µL of the Dissolution Buffer (1M TEAB).5% Hydroxylamine Add 50µL of the Quenching Reagent (50% hydroxylamine) to 450µL of 100mM TEAB. Preparing and Labeling Peptides with the TMT Isobaric Mass TagsNote: BSA can be used as a control sample for method optimization. Dissolve BSA to 1mg/mL using 100mM TEAB. Use 25-100µg of protein per labeling reaction. The Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells can also be used to prepare peptide digests for TMT reagent labeling.A.Preparing Whole Cell Protein Extracts1.Culture cells to harvest at least 100µg of protein per condition. For best results, culture a minimum of 2 × 106 cells.Note: Rinse cells 2-3 times with 1X PBS to remove cell culture media. Pellet cells using low-speed centrifugation(i.e., < 1000 × g) to prevent premature cell lysis.2.Lyse the cells by adding five cell-pellet volumes of Lysis Buffer (i.e., 100μL of Lysis Buffer for a 20μL cell pellet).Note: Lysis buffers such as 8M urea (Product No. 29700) in 50mM TEAB or HEPES buffer, pH 8 may be used as alternative denaturing cell lysis buffers. For urea-based lysis buffer, protein samples must be diluted to < 1M urea before digestion, and the final C18 desalting step (C.6) is not optional. Addition of protease and/or phosphatase inhibitors during lysis is optional and may interfere with MS analysis.Note: Depending on the Lysis Buffer used it may be necessary to reduce sample viscosity by shearing DNA using a microtip sonicator or addition of a nuclease (e.g., Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis, Product No. 88700)3.Centrifuge lysate at 16,000 × g for 10 minutes at 4°C.4.Carefully separate the supernatant and transfer into a new tube.5.Determine the protein concentration of the supernatant using established methods such as the BCA Protein Assay Kit(Product No. 23227).Note: Use samples at ≥ 2mg/mL. Less concentrated samples may be used; however, it might be necessary to use larger volumes of reducing/alkylating reagents.6.Transfer 100µg per condition (two for the TMTduplex or six for the TMTsixplex Label Reagents) into a newmicrocentrifuge tube and adjust to a final volume of 100µL with 100mM TEAB.7.Add 5µL of the 200mM TCEP and incubate sample at 55°C for 1 hour.8.Immediately before use, dissolve one tube of iodoacetamide (9mg) with 132µL of 100mM TEAB to make375mM iodoacetamide. Protect solution from light.9.Add 5µL of the 375mM iodoacetamide to the sample and incubate for 30 minutes protected from light at roomtemperature.10.Add six volumes (~600µL) of pre-chilled (-20°C) acetone and freeze at -20°C. Allow the precipitation to proceed for atleast 4 hours up to overnight.Note: Methanol/chloroform is the recommended solvent for precipitation of proteins derived from tissue extracts.11.Centrifuge the samples at 8000 ×g for 10 minutes at 4°C. Carefully invert the tubes to decant the acetone withoutdisturbing the white pellet. Allow the pellet to dry for 2-3 minutes.B.Protein Digestion1.Resuspend 100µg of acetone-precipitated (or lyophilized) protein pellets with 100µL of 50mM TEAB.Note: An acetone-precipitated pellet might not completely dissolve; however, after proteolysis at 37°C, all the protein (peptides) will be solubilized.2.Immediately before use, add 20µL of the Trypsin Storage Solution to the bottom of the trypsin glass vial and incubate for5 minutes. Store any remaining reagent in single-use volumes at -80°C (e.g., 2.5µg of trypsin per 100µg of protein).3.Add 2.5µL of trypsin (i.e., 2.5µg) per 100µg of protein. Digest the sample overnight at 37°C.C. Peptide Labeling1.Immediately before use, equilibrate the TMT Label Reagents to room temperature. For the 0.8mg vials, add 41µL ofanhydrous acetonitrile to each tube. For the 5mg vials, add 256µL of solvent to each tube. Allow the reagent to dissolve for 5 minutes with occasional vortexing. Briefly centrifuge the tube to gather the solution.Note: Reagents dissolved in anhydrous acetonitrile are stable for one week when stored at -20°C. Anhydrous ethanol can be used as an alternative solvent to dissolve reagents but is not recommended for stock solution storage.2.Optional: Measure protein digest concentration using Thermo Scientific™ Pierce™ Quantitative Fluorescent PeptideAssay (Product No. 23290) or Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay (Product No.23275).3.Carefully add 41µL of the TMT Label Reagent to each 100µL sample (25-100µg protein digest). Alternatively, transferthe reduced and alkylated protein digest to the TMT Reagent vial.4.Note: Labeling more than 100µg of protein digest per reaction requires additional TMT Label Reagent.5.Incubate the reaction for 1 hour at room temperature.6.Add 8µL of 5% hydroxylamine to the sample and incubate for 15 minutes to quench the reaction.bine samples at equal amounts in new microcentrifuge tube and store at -80°C.Note: TMT-labeled peptide concentration can be measured using Thermo Scientific™ Pierce™ QuantitativeColorimetric Peptide Assay. The Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay cannot be used to measure TMT-labeled peptide concentrations.8.Optional: Clean-up samples with C18 spin tips (Product No. 87784) or columns (Product No. 89870)before LC-MSanalysis. Peptide clean up is recommended before LC-MS analysis but is not required. Fractionation of labeled peptides using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Product No. 84868) isrecommended before LC-MS analysis to increase the number of peptide identifications.TroubleshootingProblem Possible Cause SolutionPoor labeling An amine-based buffer was used Use a non-amine-based bufferIncorrect buffer pH Make sure the buffer pH is ~8.0Too much sample was used Label 25-100µg per sampleProtein precipitation Lack of detergent present Add detergent, such as 0.05% SDS to the preparationpH decreased Make sure the pH is > 7.5Additional InformationA.Data Acquisition MethodsQuantitation of peptides labeled with Thermo Scientific™ Tandem Mass Tag™ Reagents requires a mass spectrometer capable of MS/MS fragmentation, such as an ion trap, quadrupole time of flight, time of flight-time of flight (TOF-TOF) or triple quadrupole instrument. Higher energy collision dissociation (HCD) is recommended for TMT reporter ion fragmentation. Optimal HCD fragmentation energy is instrument-dependent and can be optimized using TMTzero Reagents.Electron transfer dissociation (ETD) may be used as an alternative fragmentation method for peptide identification and quantitation. The choice of MS/MS fragmentation method(s) depends on the instrument capabilities such as collisionally induced dissociation (CID), pulsed-Q dissociation (PQD), higher energy collisional dissociation (HCD), or electron transfer dissociation (ETD). TMT Reagent reporter ions are not visible in ion traps following traditional CID fragmentation.Table 1. Instruments and MS/MS fragmentation options for peptide identification and quantitation withThermo Scientific TMT Reagents.Instrument Fragmentation Method Reference(s)Thermo Scientific Orbitrap™ Fusion™ Tribrid™ Mass Spectrometer HCD/SPS-MS3 McAllister, G.C., et al. (2014), Viner,et al. (2013)Thermo Scientific Orbitrap Elite™ Mass Spectrometer HCD/MS3 McAllister, G.C., et al. (2012), Viner,et al. (2012)Thermo Scientific Q Exactive™ MassSpectrometerHCD/MS2 Wühr, et al. (2012)Thermo Scientific Orbitrap Velos Pro™, LTQ-Orbitrap™ XL, or MALDI-Orbitrap™ XL Mass Spectrometer HCD/MS2 Ting, et al. (2011), Wenger, et al(2011), Schirle, et al. (2012), Lee, etal (2011), Xiong, et al. (2011),Strupat, et al. (2008)Thermo Scientific™ Velos Pro™ ion trap Trap HCD/MS2 Biringer, et al. (2011)Thermo Scientific Orbitrap Elite ETD, Velos Pro ETD, LTQ-OrbitrapXL ETD HCD/MS2 orETD/MS2Viner, et al. (2009)Q-TOF CID Van Ulsen, et al. (2009)TOF-TOF CID Dayon, et al. (2008)Triple Quadrupole CID/SRM Stella, et al (2011), Byers, et al.(2009)B.Data Analysis and QuantitationThe masses for peptide modification by the TMT zero, duplex, and sixplex reagents are present in the UNIMOD database () and are listed below. Several software packages directly support the modifications by TMT Reagents and the relative quantitation of reporter ions released from labeled peptides, including Thermo Scientific™ Proteome Discoverer™ 1.1 and above, Matrix Science Mascot™ 2.1 and above, and Proteome Software Scaffold™ Q+. For data acquired using a combination of fragmentation methods (i.e., HCD/MS3 or HCD/ETD), Proteome Discoverer may be necessary to merge spectra for identification and quantitation.Table 2. Modification masses of the Thermo Scientific TMT Label Reagents.Label ReagentReagentReporter IonModificationMass(monoisotopic)ModificationMass(average)HCDMonoisotopicReporter Mass*ETDMonoisotopicReporter Mass**TMT0-126 126 224.152478 224.2994 126.127726 114.127725TMT2-126 126 225.155833 225.2921 126.127726 114.127725TMT2-127 127C 225.155833 225.2921 127.131081 114.127725TMT6-126 126 229.162932 229.2634 126.127726 114.127725TMT6-127 127N 229.162932 229.2634 127.124761 115.124760TMT6-128 128C 229.162932 229.2634 128.134436 116.134433TMT6-129 129N 229.162932 229.2634 129.131471 117.131468TMT6-130 130C 229.162932 229.2634 130.141145 118.141141TMT6-131 131 229.162932 229.2634 131.138180 119.138176 * HCD is a collisional fragmentation method that generates six unique reporter ions from 126 to 131Da.**ETD is a non-ergodic fragmentation method that generates six unique reporter ions from 114 to 119Da.rmation Available from our Website•Tech Tip Protocol #49: Acetone precipitation of proteins•Tech Tip Protocol #19: Remove detergent from protein samplesRelated Thermo Scientific Products90110 TMT10plex™ Isobaric Label Reagent Set, 10 × 0.8mg90113 TMT10plex Isobaric Mass Tag Labeling Kit90406 TMT10plex Isobaric Label Reagent Set, 10 × 5mg90114 1M Triethylammonium bicarbonate (TEAB), 50mL90115 50% Hydroxylamine, 5mL90100 iodoTMTzero™ Label Reagent, 5 × 0.2mg90101 iodoTMTsixplex™ Label Reagent Set, 1 × 0.2mg90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit90076 Immobilized Anti-TMT Antibody Resin90075 Anti-TMT Antibody, 0.1mL90104 TMT Elution Buffer, 20mL84840 Pierce™ Mass Spec Sample Prep Kit for Cultured Cells23227 BCA Protein Assay Kit23275 Pierce Quantitative Colorimetric Peptide Assay23290 Pierce Quantitative Fluorescent Peptide Assay90057 Pierce Trypsin Protease, MS Grade90051 Lys-C Protease, MS Grade88300 Fe-NTA Phosphopeptide Enrichment Kit88301 Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit84868 Pierce High pH Reversed-Phase Peptide Fractionation Kit88321 Pierce Peptide Retention Time Calibration Mixture, 200µL87784 Pierce C18 Tips, 100µL bed, 96 tips89870 Pierce C18 Spin Columns, 25 columns28904 Trifluoroacetic Acid, Sequanal GradeGeneral ReferencesAltelaar A.F., et al. (2012). Benchmarking stable isotope labeling based quantitative proteomics. J Proteomics Oct 22. pii: S1874-3919(12)00704-X.doi: 10.1016/j.jprot.2012.10.009.Bantscheff, M., et al. (2008). Robust and sensitive iTRAQ quantification on an LTQ Orbitrap Mass Spectrometer. Mol Cell Proteomics7:1702-13.Biringer, R.G., et al. (2011). Quantitation of TMT-Labeled Peptides Using Higher-Energy Collisional Dissociation on the Velos Pro Ion Trap Mass Spectrometer. Application note # 520. Byers, H.L. (2009). Candidate verification of iron-regulated Neisseria meningitidis proteins using isotopic versions of tandem mass tags (TMT) and single reaction monitoring, J Prot73(2):231-9.Dayon, L., et al. (2008). Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags. Anal Chem80(8):2921-31. Dillon, R, et al. (2011). Discovery of a Novel B-Raf Fusion Protein Related to c-Met Drug Resistance. J Proteome Res10(11):5084-94.Erikson, B.K., et al. (2015). Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/orbitrap mass spectrometer. Anal Chem87(2):1241-9.Keshishian, H., et al. (2015). Multiplexed, quantitative workflow for sensitive biomarker discovery in plasma yields novel candidates for early myocardial injury. Mol Cell Proteomics. 2015 Feb 27. pii: mcp.M114.046813Lee, M.V., et al. (2011). A dynamic model of proteome changes reveals new roles for transcript alteration in yeast. Mol Syst Biol 7:514.McAllister, G.C., et al. (2014). MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem86(14):7150-8.McAllister, G.C., et al. (2012). Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal Chem 84(17):7469-78.Murphy, J.P., et al. (2014). Combining amine metabolomics and quantitative proteomics of cancer cells using derivatization with isobaric tags. Proteomics 86(7):3585-93.Paulo, J.A., et al. (2014). A comprehensive proteomic and phosphoproteomic analysis of yeast deletion mutants of 14-3-3 orthologs and associated effects of rapamycin. Nature(2-3):474-86.Ross, P.L., et al. (2004). Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 3(12):1154-69.Savitski, M.M., et al. (2014). Tracking cancer drugs in living cells by thermal profiling of the proteome. Science346(6205):1255784Schirle, M., et al. (2012). Kinase inhibitor profiling using chemoproteomics. Methods Mol Biol795:161-77.Schwartz, J. et al. (2008). Relative quantitation of protein digests using tandem mass tags and pulsed-Q dissociation (PQD). Application note # 452.Stella, R., et al. (2011). Relative Quantification of Membrane Proteins in Wild-type and PrP-knockout Cerebellar Granule Neurons. J Proteome Res doi: 10.1021/pr200759m. Strupat K., et al. (2008). Accurate MS and MSn Analysis with the Thermo Scientific MALDI LTQ Orbitrap. Application note # 30150.Ting, L., et al. (2011). MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics. Nature Methods8: 937–940.Van Ulsen, P., et al. (2009). Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. Proteomics9(7):1771-81.Viner, R.I., et al. (2013). Increasing the multiplexing of protein quantitation from 6- to 10-Plex with reporter ion isotopologues.PN_ASMS_W617_RViner_R1.Viner, R.I., et al. (2012). Relative quantitation of TMT-labeled proteomes – Focus on sensitivity and precision. Application note #566.Viner, R.I., et al. (2009). Quantification of post-translationally modified peptides of bovine α-crystallin using tandem mass tags and electron transfer dissociation. J Proteomics72(5):874-85.Wenger, C.D., et al. (2011). Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging. Nat Methods 8(11):933-5. Xiong, L., et al. (2011). Mass spectrometric studies on epigenetic interaction networks in cell differentiation. J Biol Chem 286(15):13657-68.Zhang, T., et al. (2010). Improving quantitation of TMT-labeled peptides using stepped higher-energy collisional dissociation. Application note # 483 Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts (“Documentation”). No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than Buyer. Any model or sample furnished to Buyer is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.NO OTHER WARRANTIES, EXPRESS OR IMPLIED, ARE GRANTED, INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER’S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER’S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS.Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to humans or animals.Current product instructions are available at . For a faxed copy, call 800-874-3723 or contact your local distributor.© 2016 Thermo Fisher Scientific Inc. All rights reserved. Tandem Mass Tag and TMT are trademarks of Proteome Sciences plc. iTRAQ is a trademark of AB Sciex Pte. Ltd. Mascot is a trademark of Matrix Science. Scaffold is a trademark of Proteome Software. Unless otherwise indicated, all other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Printed in the USA.。
特明科氯化胆碱（上海）有限公司介绍企业发展分析报告
Enterprise Development专业品质权威Analysis Report企业发展分析报告特明科氯化胆碱（上海）有限公司免责声明:本报告通过对该企业公开数据进行分析生成，并不完全代表我方对该企业的意见，如有错误请及时联系;本报告出于对企业发展研究目的产生，仅供参考，在任何情况下，使用本报告所引起的一切后果，我方不承担任何责任:本报告不得用于一切商业用途，如需引用或合作，请与我方联系:特明科氯化胆碱（上海）有限公司1企业发展分析结果1.1 企业发展指数得分企业发展指数得分特明科氯化胆碱（上海）有限公司综合得分说明：企业发展指数根据企业规模、企业创新、企业风险、企业活力四个维度对企业发展情况进行评价。
该企业的综合评价得分需要您得到该公司授权后，我们将协助您分析给出。
1.2 企业画像类别内容行业食品制造业-其他食品制造资质增值税一般纳税人产品服务剂，销售公司自产产品；与上述产品同类的商1.3 发展历程2工商2.1工商信息2.2工商变更2.3股东结构2.4主要人员2.5分支机构2.6对外投资2.7企业年报2.8股权出质2.9动产抵押2.10司法协助2.11清算2.12注销3投融资3.1融资历史3.2投资事件3.3核心团队3.4企业业务4企业信用4.1企业信用4.2行政许可-工商局4.3行政处罚-信用中国4.4行政处罚-工商局4.5税务评级4.6税务处罚4.7经营异常4.8经营异常-工商局4.9采购不良行为4.10产品抽查4.11产品抽查-工商局4.12欠税公告4.13环保处罚4.14被执行人5司法文书5.1法律诉讼（当事人）5.2法律诉讼（相关人）5.3开庭公告5.4被执行人5.5法院公告5.6破产暂无破产数据6企业资质6.1资质许可6.2人员资质6.3产品许可6.4特殊许可7知识产权7.1商标7.2专利7.3软件著作权7.4作品著作权7.5网站备案7.6应用APP7.7微信公众号8招标中标8.1政府招标8.2政府中标8.3央企招标8.4央企中标9标准9.1国家标准9.2行业标准9.3团体标准9.4地方标准10成果奖励10.1国家奖励10.2省部奖励10.3社会奖励10.4科技成果11土地11.1大块土地出让11.2出让公告11.3土地抵押11.4地块公示11.5大企业购地11.6土地出租11.7土地结果11.8土地转让12基金12.1国家自然基金12.2国家自然基金成果12.3国家社科基金13招聘13.1招聘信息感谢阅读:感谢您耐心地阅读这份企业调查分析报告。
Amtech Tacky 助焊膏系列安全数据表说明书
Inventec Performance Chemicals USA, LLCSAFETY DATA SHEET (SDS)SECTION 1: PRODUCT AND COMPANY IDENTIFICATIONPRODUCT NAME: Amtech Tacky Paste Flux Series: 200, 400, 500, 600, 4000, SynTECH, WSFC-305L and #61 SYNONYMS:Tacky FluxMANUFACTURER: Inventec Performance Chemicals USA, LLCADDRESS:PO Box 989 Deep River, CT 06417 USAPHONE:860-526-8300FAX:860-526-8243EMERGENCY:Infotrac-(800)535-5035REVISION DATE:December 19, 2014REVISION DATE: 3DOCUMENT NAME:SDS-Tacky Flux-008PRODUCT USE:Bonding solder joints in production and repair of circuit boardsSECTION 2: HAZARDS IDENTIFICATIONCHEMICAL NAME:N/ACHEMICAL FAMILY:MixtureCHEMICAL FORMULA:N/AROUTES OF ENTRY: Inhalation, Ingestion, Skin/Eye ContactGHS:Signal Word: WarningHazard statement(s)H302 Harmful if swallowedH317 May cause an allergic skin reactionH320 Causes eye irritationH335 May cause respiratory irritationPrecautionary statement(s)P102 Keep out of reach of childrenP233 Keep container tightly closedP264 Wash hands thoroughly after handlingP270 Do not eat, drink or smoke when using this productP280 Wear protective gloves/protective clothing/eye protection/face protectionP302+P352 IF ON SKIN: Wash with plenty of soap and waterP305+P351 IF IN EYES: Rinse continuously with water for several minutesP404 Store in a closed containerP501 Dispose of contents/containers in accordance with Federal, State/Provincial, and/or local regulations POTENTIAL HEALTH EFFECTS:EYE CONTACT: May cause moderate irritation. Do not allow material to come in contact with eyes.SKIN CONTACT: May cause moderate skin irritation.INHALATION: May cause irritation to the respiratory tract.INGESTION: Harmful if swallowed. May cause irritation to the mouth, throat, and stomach. May cause abdominal discomfort, nausea, vomiting, and/or diarrhea.CHRONIC: Not established.SECTION 2 NOTES:Inventec Performance Chemicals USA, LLC does not recommend, manufacture, market, or endorse any of its products for human consumption.SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTSIngredient CAS Number Exposure LimitsModified Rosins N/A N/APine Oil Derivatives 8000-41-7 N/AProprietary Ingredients N/A N/AMixed Carboxylic Acids N/A N/ASECTION 3 NOTES:Percentages of individual components are not listed as this information is considered a trade secret.SECTION 4: FIRST AID MEASURESEYES: Flush with plenty of water, contact a physician. If contact lenses can be removed easily, flush eyes without contact lenses. SKIN: Wash affected area with plenty of warm, soapy water. If irritation persists, seek medical attention.INGESTION: Call a physician or Poison Control Center immediately. Do not induce vomiting.INHALATION: Remove to fresh air. If not breathing, seek immediate medical attention.SECTION 5: FIRE-FIGHTING MEASURESEXTINGUISHING MEDIA: Dry chemical, foamSPECIAL FIRE FIGHTING PROCEDURES: Do not use water. Use NIOSH-approved self-contained Breathing Apparatusand full protective clothing if involved in a fire.UNUSUAL FIRE AND EXPLOSION HAZARDS:This product does not present any unusual fire and explosion hazards. SECTION 6: ACCIDENTAL RELEASE MEASURESACCIDENTAL RELEASE MEASURES: If material spills or leaks, collect and place into a properly labeled waste container. Remove traces of tacky flux using cloth rags or paper towels moistened with Isopropyl Alcohol. Follow on-site personal protective equipment recommendations.SECTION 6 NOTES:See Sections 2, 4, and 7 for additional information.SECTION 7: HANDLING AND STORAGEHANDLING/STORAGE: Keep containers tightly closed when not in use. Use care to avoid spills. Avoid inhalation of fumes or dust. Avoid contact with eyes, skin, and clothing.OTHER PRECAUTIONS: Empty containers may retain product residues in vapor, liquid, and/or solid form. All labeled hazard precautions should be observed.WORK HYGIENIC PRACTICES: Cosmetics/Food/Drink/Tobacco should not be consumed or used in work areas. Always wash hands after handling material and before applying or using cosmetics/food/drink/tobacco.SECTION 7 NOTES:For industrial use only.SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTIONVENTILATION: Provide sufficient mechanical (general and/or local exhaust) ventilation to maintain exposure below TLVs. RESPIRATORY PROTECTION: Use with adequate ventilation.EYE PROTECTION: Use with appropriate safety glasses.SKIN PROTECTION: Protective gloves and clothing should be worn when handling material. Wash hands thoroughly with soap and water upon leaving the work area.SECTION 9: PHYSICAL AND CHEMICAL PROPERTIESAPPEARANCE: Clear, White, or Yellow to Dark Amber gelODOR: Mild odorODOR THRESHOLD: Not establishedpH as SUPPLIED: N/ASECTION 9: PHYSICAL AND CHEMICAL PROPERTIES (continued)MELTING POINT: Not establishedFREEZING POINT: Not establishedINITIAL BOILING POINT: Not establishedBOILING RANGE: Not establishedFLASH POINT: Not establishedEVAPORATION RATE: Not establishedFLAMMABILITY (solid): Not establishedUPPER/LOWER FLAMMABILITY: Not establishedUPPER/LOWER EXPLOSIVE LIMITS:Not establishedVAPOR PRESSURE (mmHg): N/A (°F/°C)VAPOR DENSITY (AIR = 1): N/A (°F/°C)RELATIVE DENSITY: Not establishedSOLUBILITY IN WATER: PartiallyPARTITION COEFFICIENT (n-octanol/water): Not establishedAUTOIGNITION TEMPERATURE: Not establishedDECOMPOSITION TEMPERATURE: Not establishedVISCOSITY: N/A (°F/°C)SECTION 10: STABILITY AND REACTIVITYSTABILITY: StableCONDITIONS TO AVOID (STABILITY): Freezing temperatures. High temperatures. INCOMPATIBILITY (MATERIAL TO AVOID): Strong oxidizing materialsHAZARDOUS DECOMPOSITION/BY-PRODUCTS: Harmful organic fumes and toxic oxide fumes may form at elevatedtemperatures.POSSIBILITY OF HAZARDOUS REACTIONS: Will not occurSECTION 11: TOXICOLOGICAL INFORMATIONACUTE TOXICITY: Not availableSKIN CORRISION/IRRITATION: Not establishedSERIOUS EYE DAMAGE/IRRITATION: Not availableRESPIRATORY OR SKIN SENSITIZATION: Not establishedGERM CELL MUTAGENICITY: Not availableCARCINOGENICITY: Not availableREPRODUCTIVE TOXICITY: Not availableSTOT-SINGLE EXPOSURE: Not availableSTOT-REPEATED EXPOSURE: Not availableASPIRATION HAZARD: Not availableSECTION 12: ECOLOGICAL INFORMATIONTOXICITY: Product not testedPERSISTENCE AND DEGRADIBILITY: Product not testedBIOACCUMULATIVE POTENTIAL: Product not testedMOBILITY IN SOIL: Product not testedOTHER ADVERSE EFFECTS: Product not testedSECTION 13: DISPOSAL CONSIDERATIONSWASTE DISPOSAL METHOD: Scrap and waste solder should be stored in a dry, sealed container for later disposal. Disposal must be in accordance with Federal, State/Provincial, and Local Regulations.SECTION 14: TRANSPORT INFORMATIONTransport in accordance with applicable regulations and requirements.UN Number: Not availableUN Proper Shipping Name: Not availablePackaging Group:Not applicableEnvironmental Hazards:NoneTRANSPORT HAZARD CLASSES:US DOT Hazardous Material Classification: Tacky Flux is not listed as a DOT hazardous materialWater Transportation: Tacky Flux is not listed as a hazardous materialIATA Hazardous Material Classification: Tacky Flux is not listed as IATA hazardous materialSECTION 15: REGULATORY INFORMATIONAll ingredients used to manufacture this product are listed on the EPA TSCA Inventory.U.S. FEDERAL REGULATIONS: Not regulatedSTATE REGULATIONS: Not regulatedINTERNATIONAL REGULATIONS: Not regulatedSECTION 16: OTHER INFORMATIONHMIS Rating: Health=1 Flammability=1 Physical Hazard=0 Personal Protection=X KEY:N/A: Not applicableGHS: Global Harmonized SystemOSHA: Occupational Safety and Health AdministrationACGIH: American Conference of Governmental Industrial HygienistsNTP: National Toxicology ProgramIARC: International Agency for Research on CancerCAS: Chemical Abstract ServiceNIOSH: National Institute for Occupational Safety & HealthSTOT: Specific target organ toxicityTLV: Threshold limit valueUS DOT: United States Department of TransportationDOT: Department of TransportationIATA: International Air Transport AssociationEPA:Environmental Protection AgencyTSCA:Toxic Substance Control ActHMIS:Hazardous Material Identification SystemPREPARATION INFORMATION:This update supersedes all previously released documents.PREPARED BY: Wendy W. GesickAPPROVED BY: Leigh W. GesickDISCLAIMER:The information contained herein is based on data considered to be accurate but does not purport to be all-inclusive and shall be used only as a guide. No warranty is expressed or implied regarding the accuracy of this data and Inventec Performance Chemicals USA, LLC shall not be held liable for any damage resulting from any handling or contact with the above product. Liability is expressly disclaimed for loss or injury arising out of use of this information or the use of any materials designated. This material is not for resale, unauthorized distribution, or personal use.。
促进剂TMTM安全技术说明书97-74-5(msds)
无资料
燃爆危险：
无资料
第三部分：成分/组成信息
有害物成分：
四甲基一硫化秋兰姆；促进剂TMTM
含量：
100%
第四部分：急救措施
皮肤接触：
用肥皂水及清水彻底冲洗。就医。
眼睛接触：
拉开眼睑，用流动清水冲洗15分钟。就医。
吸入：
脱离现场至空气新鲜处。就医。
食入：
误服者，饮适量温水，催吐。就医。
第五部分：消防措施
无资料
聚合危害：
不能出现
分解产物：
无资料
第十一部分：毒理学信息
急性毒性：
LD50：818mg／kg(小鼠经口)LC50：
亚急性和慢性毒性：
无资料
RTECS：
WQl750000
刺激性：
无资料
致敏性：
无资料
致突变性：
无资料
致畸性：
无资料
致癌性：
无资料
第十二部分：生态学资料
生态毒理毒性：
无资料
生物降解性：
技术说明书编码：
XXX-MSDS-00X
供应商名称：
根据企业修改填写
供应商地址：
根据企业修改填写
供应商电话：
根据企业修改填写
供应商应急电话：
根据企业修改填写
供应商传真：
根据企业修改填写
供应商Email：
根据企业修改填写
第二部分：危险性概述
危险性类别：
无资料
侵入途径：
吸入食入经皮吸收
健康危害：
本品对粘膜、皮肤有刺激作用，对皮肤有致敏作用。误服中毒，可出现恶心、呕吐、腹痛、腹泻等症状。
第十五部分
法规信息
第八部分
tmt系统外贸实务操作答案林生贸易
操作一：建交参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548ADD: 548 YANPING ROAD，SHANGHAI 号电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeDATE: MARCH 2, 2001 Fax:065-7890023Dear Sales Manager,Through the courtesy of Bank of China, Singapore Branch, we learned that you are one of the major importers in your country of stationery and are, therefore, writing to you in the hope of establishing business relations with you.Our company is a state-owned corporation specializing in stationery and office equipment for many years. Our products, reputed for their soundquality and favorable price, are well established in markets both home and abroad. Therefore we have entered into trade relations with customers from various countries and regions in the world and business has been developing quite satisfactorily for both parties.For your interest, we would like to recommend one of our commendable products, BRIGHT brand drafting machine, which sells well in other Asian countries and is sure to find a steady demand in your country through our mutual efforts. In order to give you a brief idea of the product, we have sent you a copy of our latest catalogue for your reference. It would be in your interest to place an order with us as soon as possible, and you may get a favorable rebate for a considerable initial order.Your early reply is much appreciated.Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGERXXX操作二：报价参考商品：光明牌绘图机货号：SBT－1211.实际成本＝780×＝7202.国内费用：一个20'集装箱可装纸箱数＝25/××＝173国内费用＝10+(1000+1000+350+50+500+2000+1000)/173＝3.出口运费＝(1100×/173＝＝(720++/[ ＝＝(720+/[ ＝货号：70031.实际成本＝680×＝2.国内费用：一个20'集装箱可装纸箱数＝25/××＝285国内费用＝10+(1000+1000+350+50+500+2000+1000)/285＝3.出口运费＝(1100×/285＝＝++/[ ＝＝+/[ ＝货号：SDM-021.实际成本＝850×=2.国内费用：一个20'集装箱可装纸箱数＝25/××＝129国内费用＝10+(1000+1000+350+50+500+2000+1000)/129＝3.出口运费＝(1100×/129＝＝++/[ ＝＝+/[ ＝操作三：发盘参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548ADD: 548 YANPING ROAD，SHANGHAI 号电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeDATE: MARCH 12, 2001Dear Madam,We are glad to have received your inquiry .You may be aware that wooden cases add to both packing fees and freight, so cheaper packing in cartons will be in your interest. We assure you the seaworthiness of our cartons. For any losses caused by defects of our products, your customers can get full refunds; for other losses, they can receive replacements of spare parts for half charge.Meanwhile, please pay attention to the following points:1)We usually finance our trade with foreign customers, esp. for first transactions, by L/C at sight . Maybe you can obtain such favorable payment terms as D/P if future business develop to our mutual satisfaction.2)If transactions are concluded on CIF or CFR basis, we do not want to put you in the trouble of contacting shipping companies or arranging shipment.3)The goods will be inspected by Shanghai Inspection Bureau. Your representative can be present if it is necessary.As you can see, the above terms and conditions take the interests of both parties into consideration , so we hope you can also accept them so as to facilitate our future business. We are now glad to make you the following offer:Unit Price(USD) .Pc./ctn.Mea. . . .FOBC5 CIFC5(US$)(cm)(kg)(kg)(pcs)(US$)SBT-121180x60x3025231737003170x50x252523285SDM-02185x65x352523129Packing:To be packed in cartons of 1 pc. eachShipment:To be effected within 30 days of receipt of the related L/C.Payment:By irrevocable L/C at sight opened through a bank acceptable to the Seller. Our accounting bank is the Bank of China, Shanghai Branch.Insurance:For 110% invoice value covering All Risks & War Risk as perdated 1/1/1981.We look forward to receiving your initial order.Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGER操作四：还价参考货号：SBT-121(设还价利润率为x)，则：107＝(720++/＝－ /107＝×100％＝%利润额为：107*(720++/ ＝（美元）/台货号：700392＝++/x＝ /92＝－ /92＝×100％＝%利润额为：92*＝（美元）/台货号：SDM-02 119＝++/x＝ /119＝×100％＝%利润额为：119*＝（美元）/台二、再次报价核算参考答案货号：SBT－121CIFC5＝(720++/[＝FOBC5＝(720+/[货号：7003 CIFC5＝++/[＝FOBC5＝+/[货号：SDM-02 CIFC5＝++/[＝FOBC5＝+/[＝操作五：还盘参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548号ADD: 548 YANPING ROAD，SHANGHAI电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeDate: March 22, 2001Dear Madam,Thank you for your fax, but we are regretful to learn of your opinion on our quotatioFor your information, Japanese makers usually ask for full charges for replacement major parts, and South Korean suppliers commonly effect shipment 60 days after receipt the related Ls/C. So which one would you like to choose: a lower price with condition service, or a higher price with complete and dependable serviceBut since this is going to be the first transaction between us and our products a new to your customers, we are ready to make you the following new offer:Art. No.Unit Price CIFC5 SINGAPOREFOBC5SHANGHAISBT-1217003SDM-02Since our manufacturing department has already received a lot of indents, please pla your order with us as soon as possible.Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGERXXX操作六：成交参考总货款收入：（成交总额）285×94＋129××＝总购货成本:285×680＋129×850＝总退税收入：×9％/(1+17%)＝出口运费：1100×2×＝出口保险费：×110％×%＝客户佣金：×5％＝国内费用：10×（285＋129）＋5900×2＝总利润额：＋－－－－－＝利润率：÷×100%＝5%操作七：合同参考 <1>上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548号ADD: 548 YANPING ROAD，SHANGHAI电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeDate: April 1st 2001Dear Madam,We are pleased to book your order and are enclosing the Sales Confirmation No. LS-BRTSC in duplicate. Please sign and return one copy for our file.Meanwhile, please ask your banker to open the relevant L/C without delay so that y can receive the goods on time.Thank for your cooperation.With best regard.Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGERXXX操作七：合同参考 <2>SALES CONFIRMATIONS/C No.: LS-BRTSC07Date: April 1st 2001The Seller:SHANGHAI LINSHENG TRADING CO. LTD The Buyer: BRIGHT STATIONERY CO.Address:548 YANPING ROAD Address: 125 SUNFLOWER PLAZASHANGHAI,SINGAPO CHINACommodity & SpecificationsQUAN...MEAS.UnitPrice(US$)AMOUNT(US$)BRIGHT BRAND DRAFTING MACHINE(SET)(KG)(KG)(CM)CIFC3 NEWYORK. 7003 . SDM-022********X50X25 129252385X65X35TOTAL CONTRACT VALUE:SAY US DOLLAR FORTY-TWO THOUSAND SEVEN HUNDREDEIGHTY-EIGHT AND CENTS FIFTY-EIGHT ONLYPACKING:TO BE PACKED IN CARTONS OF ONE EACH, TOTAL 414 CARTONS ONLY.SHIPMENT:TO BE EFFECTED BY THE SELLER FROM SHANGHAI TO SINGAPORENOT LATER 31TH MAY,2001 WITH PARTIALSHIPMENT AND TRANSHIPMENT NOT ALLOWED.PAYMENT:THE BUYER SHALL OPEN THROUGH A BANK ACCEPTABLE TO THESELLER AN IRREVOCABLE SIGHT LETTER OF CREDIT TO REACH THESELLER BEFORE APRIL 25 2001 AND REMAIN VALID FOR NEGOTIATION INCHINA UNTIL THE 15TH DAY AFTER THE TIME OF SHIPMENT.THE SELLER SHALL COVER THE INSURANCE FOR 110% OF TOTAL INVOICE INSURANCE:VALUE AGAINST ALL RISKS AND WAR RISKS AS PER AND SUBJECT TORELEVANT OCEAN MARINE CARGO CLAUSES OF PEOPLE'S INSURANCECOMPANY OF CHINA DATED 1/1/1981.Confirmed by:THE SELLER THE BUYERSHANGHAI LINSHENG TRADING CO. LTD.XXX操作八：审证参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548号ADD: 548 YANPING ROAD，SHANGHAI电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－审证意见信用证存在的问题需要修改的理由国外到期易产生逾期交单受益人地址有误易出现单、证不符信用证金额有误无法全额出运货物投保加成错误增加保费支出投保依据的条款制订的日期有误与实际不符包装条款不妥增加包装支出操作九：改证参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548号ADD: 548 YANPING ROAD，SHANGHAI电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeFax:065-7890023Date: April 25, 2001Dear Madam,Thank you for your L/C . However, we find it contains the following discrepancies:1. THE CREDIT IS TO EXPIRE IN CHINA, NOT IN SINGAPORE.2. THE ADDRESS OF THE APPLICANT SHOULD BE '125 SUNFLOWER PLAZA', NOT '521 SUNFLOWERPLAZA'.3. THE AMOUNT SHOULD BE 'SAY US DOLLARS FORTY-TWO THOUSAND SEVEN HUNDRED EIGHTY-EIGHT AND CENTS FIFTY-EIGHT ONLY'.4. INSURANCE IS TO BE COVERED FOR 110% OF INVOICE VALUE, NOT FOR FULL INVOICE VALUE PLUS 110%.5. THE CIC SHOULD BE DATED 01/01/1981, INSTEAD OF 01/01/1980.6. GOODS SHOULD BE PACKED IN CARTONS, NOT IN WOODEN CASES.Please ask your banker to amend the L/C accordingly, and please see to it that the L/C amendment reach us before April 30, 2001, so that you can receive the goods without delay.With Best Regards.Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGERXXX操作十： 订舱参考 <1>公司编号: BH07LS 出 口 货 物 订 舱 委 托 书 日期: 9-May-011)发货人SHANGHAI LINSHENG TRADING CO. LTD.548 YANPING ROADSHANGHAI CHINA 4)信用证号码 BRT-LSLC075)开证银行 DBS BANK 6)合同号码 LS-BRTSC07 7)成交金额 US$8) 装运口岸 SHANGHAI9)目的港 SINGAPORE2)收货人 TO ORDER10)转船运输 NO11)分批装运 12)信用证效期 15-Jun-01 13)装船期限 31-May-0114)运费 PREPAID15)成交条件 CIFC5SINGAPORE16)公司联系人 XXX17)电话/传真 021－3)通知人BRIGHT STATIONERY 125 SUNFLOWER PLAZA,SINGAPORE 18)公司开户行 Bank ofChina19)银行帐号 3520)特别要求21)标记唛码 22)货号规格 23)包装件数 24)毛重 25)净重 26)数量 27)单价 28)总价BRIGHT BRAND DRAFTING MACHINES CIFC5 SINGAPOREBRIGHT7003285CTNS7125KGS 6555KGS 285SETS USD94SINGAPORE SDM02129CTNS3225KGS2967KGS129SETSC/NO. 1-41429)总件数30)总毛重31)总净重32)总尺码33)总金额414CTNS10350KGS9522KGS US$34)备注操作十：订舱参考 <2> C O M M E R C I A L I NV O I C E1) SELLER3) INVOICE NO.4) INVOICE DATE SHANGHAI LINSHENG TRADING .LS-BRTINV079-May-01548 YANPING ROAD5) L/C NO.6) DATE SHANGHAI CHINA BRT-LSLC0714-Apr-01ISSUED BYDBS BANK2) BUYER8) CONTRACT NO.9) DATEBRIGHT STATIONERY CO LS-BRTSC071-Apr-01125 SUNFLOWER PLAZA10) FROM11) TO SINGAPORE SHANGHAI SINGAPORE12) SHIPPED BY13) PRICE TERMCIFC5 SINGAPORE18)14) MARKS15) DESCRIPTION OF GOODS 16) QTY.17) UNIT PRICEAMOUNT(USD)BRIGHT BRAND DRAFTINGCIFC5 SINGAPORE MACHINESBRIGHT7003285SETSSINGAPORE SDM02129SETSC/TOTAL:19)TOTAL VALUESAY US DOLLARS FORTY-TWO THOUSAND SEVEN HUNDRED EIGHTY-EIGHT AND FIFTY EIGHT ONLY20) CERTIFICATIONAS PER SALES CONFIRMATION21)PACKING7003 TO BE PACKED IN 285 CTNS OF ONE EACHSDM02 TO BE PACKED IN 129 CTNS OF ONE EACHTATAL FOUR HUNDRED AND FOURTEEN CARTOONS ONLY22)GROSS WEIGHT10350KGSL/C NO. BRT-LSLC0723) ISSUED BYSHANGHAI LINSHENG TRADING CO. LTD.24) SIGNATUREXXX6 COPIES操作十：订舱参考 <3>P A C K I N G L I S T1) SELLER3) INVOICE NO.4) INVOICE DATE SHANGHAI LINSHENG TRADING .LS-BRTINV079-May-01548 YANPING ROAD 5) FROM 6) TO SHANGHAI CHINA SHANGHAISINGOPORE7) TOTAL PACKAGES(IN WORDS)FOUR HUNDRED AND FOURTEEN CARTONS ONLY 2) BUYER8) MARKS & NOS. BRIGHT STATIONERY BRIGHT 125 SUNFLOWER PLAZA SINGAPORE SINGAPORE C/9) C/NOS. 10) NOS. & KINDS OF PKGS. 11) ITEM 12)QTY. 13) .(kg) 14) .(kg)15)MEAS(m3)BRIGHT BRAND DRAFTINGMACHINES1-285285 CTNS7003 285 SETS 7125 KGS 6555 KGS M3 286-414 129 CTNS SDM02 129 SETS 3225 KGS 2967 KGSTOTAL: 414 CTNS 414 SETS 10350 KGS 9522 KGSL/C NO. BRT-LSLC0716) ISSUED BYSHANGHAI LINSHENG TRADING CO. LTD.17) SIGNATUREXXX3 COPIES操作十一：报关参考中华人民共和国海关出口货物报关单预录入编号:海关编号:操作十二：投保参考海运出口货物投保单1)保险人：2)被保险人：中国人民保险公司上海林生贸易有限公司8)运输工具:(船名)(航次)HAILING9)装运港:SHANGHAI10)目的港:SINGAPORE11)投保险别:12)货物起运日期:20-May-01 ALL RISKS AND WAR RISKS AS PEROCEAN MARINE CARGO CLAUSESOF PICC DATED 1/1/1981.13)投保日期：14)投保人签字：15-May-01上海林生贸易有限公司XXX操作十二：装船参考上海林生贸易有限公司Shanghai Linsheng Trading Co., Ltd.地址:中国上海延平路548号ADD: 548 YANPING ROAD，SHANGHAI电话：0086－021－TEL: 0086－021－传真：0086－021－FAX: 0086－021－Sales ManagerBright Stationery Co.125 Sunflower PlazaSingaporeDATE: May 15th 2001Dear Madam,Thank you for your fax.This is to inform you that 414 cartons of Bright Brand drafting machines under S/C No. LS-BRTSC07 have been loaded on board . Hailing , which will be sailing on May 20, 2001 The B/L No. is LS-BRTBL07, and the total invoice value is US$42,. We are confident that you will find the goods totally satisfactory to your customers.With best regards!Yours sincerely,SHANGHAI LINSHENG TRADING CO. LTD.MANAGERXXX操作十三：议付参考 <1>BILL OF EXCHANGENo.LS-BRTINV07For US$SHANGHAI27-May-01(amount in figure)(place and date of issue)At*****************sight of this F IRST Bill of exchange(SECOND being unpai pay to BANK OF CHINA, SHANGHAI BRANCH or order the sum ofUS DOLLARS FORTY-TWO THOUSAND SEVEN HUNDRED EIGHTY-EIGHT AND CENTS FIFTY-EIGHT ONLY(amount in words)Value received for414 CARTONS of DRAFTING MACHINES( quantity )( name of commodity )Drawn under DBS BANKL/C No.BRT-LSLC07dated14-Apr-01To:DBS BANK For and on behalf ofTRADE SERVICES DEPARTMENT SHANGHAI LINSHENG TRADING CO. LTD.6 SHENTON WAY, DBS BUILDING TOWER TWOSINGAPORE, 0106XXX(Signature)操作十三： <2>B I L L O F L A D I N G11)MARKS 12) NOS. & KINDS OF PKGS. 13)DESCRIPTION OF GOODS14) .(kg)15) MEAS(m3)BRIGHT414 CARTONS BTIGHT BRAND DRAFTING MACHINES10350SINGAPORE ART. NO. 7003C/NO. 1-414ART. NO. SDM-02FREIGHT PREPAIDL/C16)TOTAL NUMBER OF CONTAINERSSAY FOUR HUNDRED AND FOURTEEN CARTONS ONLYOR PACKAGES(IN WORDS)19)DATE20-May-0120)BYCOSCO-MIDSON SHIPPING INC林自强林自强ENDORSEMENT: SHANGHAI LINGSHENG TRADING .X X X 3 COPIES操作十三：议付参考 <3>C O M M E R C I A L I N V O I C E1) SELLER3) INVOICE NO.4) INVOICE DATE SHANGHAI LINSHENG TRADING .LS-BRTINV079-May-01548 YANPING ROAD5) L/C NO.6) DATESHANGHAI CHINA BRT-LSLC0714-Apr-01ISSUED BYDBS BANK2) BUYER8) CONTRACT NO.9) DATEBRIGHT STATIONERY CO LS-BRTSC071-Apr-01125 SUNFLOWER PLAZA10) FROM11) TOSINGAPORE SHANGHAI SINGAPORE12) SHIPPED BY13) PRICE TERMHAILING CIFC5 SINGAPORE14) MARKS15) DESCRIPTION OF GOODS 16) QTY.17) UNIT PRICE18) AMOUNT(USD)BRIGHT BRAND DRAFTING MACHINES CIFC5 SINGAPOREBRIGHT7003285SETSSINGAPORE SDM02129SETSC/TOTAL:19)TOTAL VALUESAY US DOLLARS FORTY-TWO THOUSAND SEVEN HUNDRED EIGHTY-EIGHT AND FIFTY EIGHT ONLY20) CERTIFICATIONAS PER SALES CONFIRMATION21)PACKING7003 TO BE PACKED IN 285 CTNS OF ONE EACHSDM02 TO BE PACKED IN 129 CTNS OF ONE EACHTATAL FOUR HUNDRED AND FOURTEEN CARTOONS ONLY22)GROSS WEIGHT10350KGSL/C NO. BRT-LSLC0723) ISSUED BYSHANGHAI LINSHENG TRADING CO. LTD.24) SIGNATUREXXX5 COPIES操作十三：议付参考 <4>中国人民保险公司THE PEOPLE'S INSURANCE COMPANY OF CHINA总公司设于北京一九四九年创立Head office: BEIJING Established in 1949保险单保险单号次INSURANCE POLICY POLICY NO.LS-BRTB中国人民保险公司 ( 以下简称本公司 )THIS POLICY OF INSURANCE WITNESSES THAT THE PEOPLE'S INSURANCE COMPANY OF CHINA ( HEREINAFTER CA COMPANY" )根据AT THE REQUEST OF SHANGHAI LINSHENG TRADING .(以下简称被保险人 ) 的要求，由被保险人向本公司缴付约( HEREINAFTER CALLED " THE INSURED " ) AND IN CONSIDERATION OF THE AGREED PREMIUM PAID TO THE COMP定的保险，按照本保险单承保险别和背面所载条款下列INSURED UNDERTAKES TO INSURE THE UNDERMENTIONED GOODS IN TRANSPORTATION SUBJECT TO THE CONDITIONS OF T 特款承保下述货物运输保险，特立本保险单AS PER THE CLAUSES PRINTED OVERLEAF AND OTHER SPECIAL CLAUSES ATTACHED HEREONBRIGHT414 CARTONS DRAFTING MACHINES US$SINGAPOREC/总保险金额:TOTAL AMOUNT INSURED:SAY US DOLLARS FORTY-SEVEN THOUSAND AND SIXTY-EIGHT ONLY保费费率装载运输工具PREMIUM AS ARRANGED RATE AS ARRANGED PER CONVEYANCE SS.HAILING开航日期自至SLG. ON OR ABT.AS PER BILL OF LADING FROM SHANGHAI TO SINGAPO 承保险别:COVERING ALL RISKS AND WAR RISKS FOR 110% OF INVOICE VALUE AS PER AND SUBJEC CONDITIONSMARINECARGO CLAUSES OF THE PEOPLES INSURANCE COMPANY OF CHINA DATED 1/1/1981.L/C NO. BRT-LSLC07所保货物，如遇出险，本公司凭本保险单及其他有关证件给付赔款。
tmt 行业报告 2018
tmt 行业报告 2018TMT Industry Report 2018。
The TMT (Technology, Media, and Telecommunications) industry has seen significant growth and transformation in 2018. This report aims to provide an overview of the key trends, challenges, and opportunities that have shaped the industry over the past year.Technology。
In the technology sector, 2018 has been marked by the continued rise of artificial intelligence (AI), machine learning, and big data analytics. Companies across various industries have increasingly adopted these technologies to improve efficiency, enhance customer experiences, and drive innovation. The development of 5G networks has also been a major focus, with telecommunications companies investing heavily in infrastructure to support the next generation of wireless technology.In addition, cybersecurity has become a top priority for organizations as the frequency and sophistication of cyber attacks continue to increase. This has led to a growing demand for advanced security solutions and services, as well as greater investment in cybersecurity measures.Media。
有机硫TMT-15技术指标
产品概括:有机硫是一种含硫化合物，它通过硫族与重金属离子稳定结合并发生化学反应，形成稳定的有机金属化合物，不易溶解，在水溶液中形成固体沉淀，进而被分离去除。
这是一种化学计量反应。
在重金属族中，汞和镉形成的重金属化合物具有最低的溶解度。
在烟气湿法脱硫中有机硫主要用于废水中重金属沉降剂。
产品特点：1、具有强大的螯合力能有效地与重金属发生化学反应生成不溶物，尤其是汞、镉，主要应用于湿法硫工艺过程中；2、几乎能吸附所有的重金属，尤其在废水处理中，通过简单的处理可以去除所有溶解的残留重金属;3、金属—沉淀物具有良好的温度稳定性，重金属很难重新释放到环境中去，是环境友好的重金属捕捉剂;4、具有良好的毒理学和生物学特性，其毒性很低;5、具有良好的存储稳定性和操作安全性，不属于危险物品，无不良气味，不分解出有毒物质。
重金属污染：常见的重金属污染有：汞（Hg）、（Pb）、镉（Cd）、锰（Mn）、铬（Cr）、铜（Cu）、镍（Ni）、锌（Zn）等由于在工业加工过程中重金属又通常不能替换，所以造成废水中重金属离子超标，因其具有毒性，超标排放后不能被自然分解，所以重金属离子在废水和废气中的含量是被国家严格限制的。
产品分类及性能指标TMT-15(有机硫)项目指标密度(g/ml)20 ℃≥1.12适用PH范围3—13浓度≥15%气味无异味，略带芳香味外观淡绿或淡黄色液体TMT-15产品使用方法①先检测废水中的重金属离子的含量，再通过废水模拟加药试验确定药剂的最佳加入量。
②药剂加入前要先将废水中的PH值调至7-9之间效果最佳。
③根据每天需处理的废水量确定当天总的投放量，投放前将量取的药剂在专用的加药系统中稀释成1-2%的浓度，然后连续或分批投放废水中，处理时废水与TMT-18必须充分搅拌均匀，搅拌时间约为5-10分钟，并加入适量的絮凝剂、助凝剂一起搅拌。
④搅拌后的重金属沉淀物与水分离前一般静置10分钟，然后将重金属沉淀物经压滤机过滤压饼，含重金属的滤饼作为无浸出毒性的废物深埋处理，过滤后的水可达标排放。
TMT国际贸易实训,上海杰依工贸出口报价
上海杰依工贸有限公司货号：SY0011）实际成本＝采购成本－退税收入＝34.90-34.90×9%/(1+17%）＝32.2154元/条2） 20英尺集装箱装箱量：25/(1.02×0.525×0.31）＝150.5979，取整，150箱报价数量：150×20＝3000 打3）国内费用：5+(500+1000+350+50+1000+500+2000+1000）/3000＝7.1333元/条4）出口运费＝(2700×8.25）/3000＝7.425元/条5）出口报价：FOBC3＝（实际成本+国内费用）/（1-佣金率-预期利润率）＝(32.2154+7.1333)/(1-3%-15%)/8.25＝ 5.82美元/条CFRC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率）＝(32.2154+7.1333+7.425)/(1-3%-15%)/8.25＝6.91美元/条CIFC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率-（1+加成率）×保费率）＝(32.2154+7.1333+7.425)/(1-3%-15%-110%×(0.6%+0.3%))/8.25＝7.00美元/条货号：BS0071）实际成本＝采购成本－退税收入＝36.70-36.70×9%/(1+17%）＝33.8769元/条2） 20英尺集装箱装箱量：25/(1.02×0.525×0.31）＝150.5979，取整，150箱报价数量：150×20＝3000 打3）国内费用：5+(500+1000+350+50+1000+500+2000+1000）/3000＝7.1333元/条4）出口运费＝(2700×8.25）/3000＝7.425元/条5）出口报价：FOBC3＝（实际成本+国内费用）/（1-佣金率-预期利润率）＝(33.8769+7.1333)/(1-3%-15%)/8.25＝6.06美元/条CFRC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率）＝(33.8769+7.1333+7.425)/ /(1-3%-15%)/8.25＝7.16美元/条CIFC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率-（1+加成率）×保费率）＝(33.8769+7.1333+7.425)/(1-3%-15%-110%×(0.6%+0.3%))/8.25＝57.25美元/条货号：WP1011）实际成本＝采购成本－退税收入＝40.20-40.20×9%/(1+17%)＝37.1077元/条2) 20英尺集装箱装箱量：25/(1.05×0.54×0.33)＝133.6112，取整，133箱报价数量：133×20＝2660 打3) 国内费用：5+(500+1000+350+50+1000+500+2000+1000)/2660＝7.4060元/条4) 出口运费＝(2700×8.25)/2660＝8.3741元/条5) 出口报价：FOBC3＝（实际成本+国内费用）/（1-佣金率-预期利润率）＝(37.1077+7.4060)/(1-3%-15%)/8.25＝6.58美元/条CFRC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率）＝(37.1077+7.4060+8.3741)/ /(1-3%-15%)/8.25＝7.82美元/条CIFC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率-（1+加成率）×保费率）＝(37.1077+7.4060+8.3741)/（1-3%-15%-110%×(0.6%+0.3%)）/8.25＝7.91美元/条货号：AF0221) 实际成本＝采购成本－退税收入＝44.50-44.50×9%/(1+17%)＝41.0769元/条2) 20英尺集装箱装箱量：25/(1.10×0.56×0.34)＝119.3659，取整，119箱报价数量：119×20＝2380 打3) 国内费用：5+(500+1000+350+50+1000+500+2000+1000)/2380＝7.6891元/条4) 出口运费＝(2700×8.25)/2380＝9.3592元/条5) 出口报价：FOBC3＝（实际成本+国内费用）/（1-佣金率-预期利润率）＝(41.0769+7.6891)/ /(1-3%-15%)/8.25＝7.21美元/条CFRC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率）＝(41.0769+7.6891+9.3592)/(1-3%-15%)/8.25＝8.59美元/条CIFC3＝（实际成本+国内费用+出口运费）/（1-佣金率-预期利润率-（1+加成率）×保费率）＝(41.0769+7.6891+9.3592)/（1-3%-15%-110%×(0.6%+0.3%)）/8.25＝8.70美元/条。
Artisan Technology Group 产品说明书
Agilent 16048G/H Test Leads Operation and Service ManualThird EditionAgilent Part No. 16048-90050January 2001Printed in: JapanNoticesThe information contained in this document is subject to change without notice.This document contains proprietary information that is protected by copyright.Allrights are reserved. No part of this document may be photocopied, reproduced, ortranslated to another language without the prior written consent of the AgilentTechnologies.Agilent Technologies Japan, Ltd.Component Test PGU-Kobe1-3-2, Murotani, Nishi-Ku, Kobe-shi, Hyogo, 651-2241 Japan© Copyright Agilent Technologies Japan, Ltd. 1999, 2001Manual Printing HistoryThe manual’s printing date and part number indicate its current edition. Theprinting date changes when a new edition is printed. (Minor corrections andupdates that are incorporated at reprint do not cause the date to change.) Themanual part number changes when extensive technical changes are incorporated.April 1999 First Edition (part number: 16048-90050)December 1999 Second Edition (part number: 16048-90050)January 2001 Third Edition (part number: 16048-90050)Safety SummaryThe following general safety precautions must be observed during all phases ofoperation, service, and repair of this instrument. Failure to comply with theseprecautions or with specific WARNINGS elsewhere in this manual may impair theprotection provided by the equipment. In addition it violates safety standards ofdesign, manufacture, and intended use of the instrument.The Agilent Technologies assumes no liability for the customer’s failure to complywith these requirements.NOTE16048G/H comply with INSTALLATION CATEGORY I and POLLUTIONDEGREE 2 in IEC61010-1.16048G/H are INDOOR USE product.2•DO NOT Operate In An Explosive AtmosphereDo not operate the instrument in the presence of flammable gasses or fumes.Operation of any electrical instrument in such an environment constitutes adefinite safety hazard.•Keep Away From Live CircuitsOperating personnel must not remove instrument covers. Componentreplacement and internal adjustments must be made by qualified maintenancepersonnel. Do not replace components with the power cable connected. Undercertain conditions, dangerous voltages may exist even with the power cableremoved. To avoid injuries, always disconnect power and discharge circuitsbefore touching them.•DO NOT Service Or Adjust AloneDo not attempt internal service or adjustment unless another person, capable ofrendering first aid and resuscitation, is present.•DO NOT Substitute Parts Or Modify InstrumentBecause of the danger of introducing additional hazards, do not installsubstitute parts or perform unauthorized modifications to the instrument.Return the instrument to a Agilent Technologies Sales and Service Office forservice and repair to ensure that safety features are maintained.•Dangerous Procedure WarningsWarnings, such as the example below, precede potentially dangerousprocedures throughout this manual. Instructions contained in the warnings mustbe followed.WARNING Dangerous voltages, capable of causing death, are presenting this instrument.Use extreme caution when handling, testing, and adjusting this instrument.CertificationAgilent Technologies certifies that this product met its published specifications atthe time of shipment from the factory. Agilent Technologies further certifies thatits calibration measurements are traceable to the United States National Institute ofStandards and Technology, to the extent allowed by the Institution’s calibrationfacility, or to the calibration facilities of other International Standards Organizationmembers.3WarrantyThis Agilent Technologies instrument product is warranted against defects inmaterial and workmanship for a period corresponding to the individual warrantyperiods of its component products. Instruments are warranted for a period of oneyear. Fixtures and adapters are warranted for a period of 90 days. During thewarranty period, Agilent Technologies will, at its option, either repair or replaceproducts that prove to be defective.For warranty service or repair, this product must be returned to a service facilitydesignated by Agilent Technologies. Buyer shall prepay shipping charges toAgilent Technologies and Agilent Technologies shall pay shipping charges toreturn the product to Buyer. However, Buyer shall pay all shipping charges, duties,and taxes for products returned to Agilent Technologies from another country.Agilent Technologies warrants that its software and firmware designated byAgilent Technologies for use with an instrument will execute its programminginstruction when property installed on that instrument. Agilent Technologies doesnot warrant that the operation of the instrument, or software, or firmware will beuninterrupted or error free.Limitation Of WarrantyThe foregoing warranty shall not apply to defects resulting from improper orinadequate maintenance by Buyer, Buyer-supplied software or interfacing,unauthorized modification or misuse, operation outside the environmentalspecifications for the product, or improper site preparation or maintenance. IMPORTANT No other warranty is expressed or implied. Agilent Technologies specificallydisclaims the implied warranties of merchantability and fitness for a particularpurpose.Exclusive RemediesThe remedies provided herein are buyer’s sole and exclusive remedies. AgilentTechnologies shall not be liable for any direct, indirect, special, incidental, orconsequential damages, whether based on contract, tort, or any other legal theory.45AssistanceProduct maintenance agreements and other customer assistance agreements areavailable for Agilent Technologies products.For any assistance, contact your nearest Agilent Technologies Sales and Service Office. Addresses are provided at the back of this manual.Safety SymbolGeneral definitions of safety symbols used on the instrument or in manuals arelisted below.Instruction Manual symbol: the product is marked with this symbol when it isnecessary for the user to refer to the instrument manual.Alternating current.Direct current.On (Supply).Off (Supply).In position of push-button switch.Out position of push-button switch.Frame (or chassis) terminal. A connection to the frame (chassis) of the equipment which normally include all exposed metal structure.WARNING This warning sign denotes a hazard. It calls attention to a procedure, practice, condition or the like, which, if not correctly performed or adhered to, could result in injury or death to personnel.CAUTION This Caution sign denotes a hazard. It calls attention to a procedure, practice, condition or the like, which, if not correctly performed or adhered to, could result in damage to or destruction of part or all of the product.NOTE Note denotes important information. It calls attention to a procedure, practice,condition or the like, which is essential to highlight.6Contents1.Installation GuideIncoming Inspection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Connecting the 16048G/H . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.OverviewProduct Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 3.Operation4294A Setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Adapter Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Performing Fixture Compensation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4. SpecificationsSpecifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 5.ServiceMaintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247Contents 81Installation Guide9Installation GuideIncoming InspectionIncoming InspectionInspect the shipping container for damage. If the shipping container or cushioningmaterial is damaged, it should be kept until the contents of the shipment have beenchecked for completeness and the 16048G/H has been checked mechanically andelectrically. The contents of the shipment should be as listed in Table 1-1. If thecontents are incomplete, if there is mechanical damage or defect, notify the nearestAgilent Technologies office. If the shipping container is damaged, or thecushioning material shows signs of unusual stress, notify the carrier as well as theAgilent Technologies office. Keep the shipping materials for the carrier’sinspection.Table 1-1 ContentsDescription Part Number Qty.Test Lead (16048G or 16048H)-1Mounting Plate-1BNC Bracket a16048-600031Operation and Service Manual16048-900501a.Only furnished with option 001.10Chapter 1Chapter 111Installation GuideConnecting the 16048G/HConnecting the 16048G/HFollow these steps below to connect the 16048G or 16048H to the 4294A.Step 1.Set the 16048G/H to the test connectors on the front panel of the HP 4294A bygradually coupling the four BNC connectors and fastening screws of the fixture with the test connectors and accessory mounting holes of the instrument until they come to complete contact. Step 2.Fasten two of the four BNC connectors to the mating test connectors by graduallyturning the BNC connectors' rotation levers until each pair of connectors are securely connected. Be sure to align the grooves on both sides. Step 3.Turn clockwise the fixture's two fastening screws together, so that the fixture issecured to the instrument.Step 4.Finally, secure the remaining two BNC connectors of the fixture by turningclockwise their rotation levers.Figure 1-1Connecting the fixture to the instrumentNOTEUsing the connection block securing screws enables the 16048G/H to connect the GND of the 4294A.Installation GuideConnecting the 16048G/H12Chapter 12Overview1314Chapter 2OverviewProduct OverviewProduct OverviewThe 16048G/H consists of a direct attachment, 4-terminal pair interface which is equipped with four BNC (f) connectors. These test leads are used to attach user-fabricated testfixtures. Cable length of the 16048G is 1 meter, the 16048H is 2 meter.Figure 2-1Product OverviewChapter 215Overview FunctionsFunctionsFigure 2-2 shows name of each part of the 16048G/H and Table 2-2 shows their function.Figure 2-216048G/H PartsTable 2-1 16048G/H FunctionFUNCTION1Instrument Side Connection Block Connect to the instrument’s UNKNOWN terminal.2Connection Block Securing Screw Secures the connection block to the 4294A.3Ground Lead Connect to the chassis or grounded part of the measurement target.4DUT Side Connection Block Used to attach BNC Bracket or user-fabricated test fixtures.5Mounting PlateMounts on the measurement target to stabilize the cable.OverviewFunctionsCable AssignmentFigure 2-316Chapter 23OperationThis chapter describes the proper methods for setting the 4294A, fixturecompensation with the 16048G/H.1718Chapter 3Operation4294A Setting4294A SettingBefore you begin your measurement, you should perform the adapter setup in the 4294A. Also refer to the operation manual of the 4294A about the adapter setup.Adapter SetupConnect the 16048G/H to the 4294A and perform the adapter setup described below.NOTEFor adapter setup, use the 100 Ω Resistor furnished with the 4294A (Agilent P/N 04294-61001).1.More than 30 minutes warm-up time is required after turning on the 4294A.2.Press [Cal] key to bring up the Calibration Menu.3.Press ADAPTER [] key to bring up the adapter setup Menu. [ ] shows current settings.4.Select 4TP 1M in the case of 16048G, 4TP 2M in the 16048H. When the selection is completed, the softkey label will be underlined.5.Press SETUP key to bring up the Adapter Setup Menu.6.Connect the Lcur terminal and Lpot terminal on the 16048G or 16048H to the Hpot terminal and Hcur terminal of the 100 Ω Resistor furnished with the 4294A. The Hcur and Hpot terminal on the 16048G/H should remain open (nothing should be connected).Figure 3-1 Connecting 100 Ω Resister (Phase Compensation Data Measurement)Chapter 319Operation 4294A Setting7.Press PHASE COMP [-] key to start the phase compensation data measurement.About 1 minutes later, phase compensation data measurement is completed andthe softkey label changes to PHASE COMP [DONE].8.Connect the Lcur, Lpot, Hcur, Hpot terminal on the 16048G or 16048H to the Lcur, Lpot, Hcur, Hpot terminal of the 100 Ω Resistor respectively.Figure 3-2Connecting 100 Ω Resister (Load Data Measurement)9.Press LOAD [-] key to start the load data measurement. When the load datameasurement is completed, the softkey label changes to LOAD [DONE].10.Press donekey.OperationPerforming Fixture CompensationPerforming Fixture CompensationTo compensate stray capacitance and residual impedance, fixture compensation should be performed. When you use the optional BNC Bracket (Agilent P/N 16048-60003) or an user fabricated test fixture, fixture compensation is required. Refer to the4294A Operation Manual for the fixture compensation procedure.20Chapter 34 SpecificationsThis chapter provides specifications of the16048G/H Test Leads.21SpecificationsSpecificationsSpecificationsApplicable Instruments LCR meters and Impedance Analyzers withfour-terminalsMaximum V oltage± 42V peak max. (AC+DC)Operating Environment temp.-20°C to +150°C(Exclude up to 20 cm from four terminal pairconnection block)humidity15% to 95%RH( @ wet bulb temp. < 40°C)Non Operating Environment.temp.-40°C to+70°Chumidity≤ 90 % RH ( @ wet bulb temp. <65°C)Cable length (Nominal) 1 m (16048G) / 2 m (16048H)Weight Approximately 460 g (16048G)Approximately 690 g (16048H)Safety Standards EN61010-1:1993 +A2:1995IEC61010-1:1990 +A1:1992 +A2:1995CSA C22.2 No.1010.1:1992INSTALLATION CATEGORY IPOLLUTION DEGREE 2INDOOR USE22Chapter 45ServiceThis chapter provides information on servicing and proper maintenance.2324Chapter 5ServiceMaintenanceMaintenanceAn exploded view of the 16048G/H for parts identification is shown in Figure 5-1 to Figure 5-4. Do not disassemble any further than shown. Maintenance consists principally of cleaning contacts and replacing worn or damaged parts. Take special care when cleaning contacts.To order parts, use the Agilent part numbers listed in Table 5-1 to Table 5-4. If a faulty part is located in an assembly that cannot be disassembled, order the nexthigher assembly or return the fixture to the nearest Agilent Technologies Sales/Service Office for repair or replacement.Figure 5-1Replacable Parts (part 1 of 4)ServiceMaintenance Table 5-1 Replacable Parts (part 1 of 4)Agilent Part No.Qty.DescriptionReferenceDesignator116048-616201CBL ASSY 16048G(Excludes 2,3,4,5,6)16048-616231CBL ASSY 16048H(Excludes 2,3,4,5,6)21400-04934CABLE TIE39170-14651CORE4-1CBL CLANP50515-15501SCR M3-L 8 P-H63050-08911WASHER M372950-00354NUT-HEX-DBL-CHAM82190-01024WASHER916048-006231GUIDE101250-02524CONN-RF BNC1116048-006261PLATE1216048-006271ANGLE130515-10122SCR FL M4L8Chapter 52526Chapter 5ServiceMaintenanceFigure 5-2Replacable Parts (part 2 of 4)Table 5-2 Replacable Parts (part 2 of 4)Reference Designator Agilent Part No.Qty.Description 116044-600012CONN BNC-SMB 23050-00674WSHR-FL MTLC 33050-07894WSHR-FL NM 416047-400024INSULATOR 51253-04762ADPT BNC-SMBChapter 527Service MaintenanceFigure 5-3 Replacable Parts (part 3 of 4)Table 5-3 Replacable Parts (part 3 of 4)Reference Designator Agilent Part No.Qty.Description 116047-240212KNOB216048-040211COVER TOP (16048G)16048-040251COVER TOP (16048H)316047-240262FLANGE40515-09144SCR-MACH M3X0.550515-09524SCR-MACH M2X0.4616048-006241ANGLE70515-09143SCR-MACH M3X0.528Chapter 5ServiceMaintenanceFigure 5-4 Replacable Parts (part 4 of 4)816048-040221COVER BOTTOM 916048-006251ANGLETable 5-4 Replacable Parts (part 4 of 4)Reference Designator Agilent Part No.Qty.Description 11250-19854CONNECTOR-BNC 216048-006211PLATE 32190-00164WASHER42950-00434NUT-HEX-DBL-CHAM 516380-240014TERMINAL 642036-006012PLATE 73050-08934WASHER 80535-00434NUTTable 5-3 Replacable Parts (part 3 of 4)Reference Designator Agilent Part No.Qty.Description。
Protocol _TMT labeling 6-plex
INSTRUCTIONSTMT® Mass Tagging Kits and Reagents90060 TMTduplex™ Isotopic Label Reagent Set, sufficient reagents for 5 duplex isotopic experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mg90061 TMTsixplex™Isobaric Label Reagent Set, sufficient reagents for 1 sixplex isobaric experiment Contents:TMT6-126 Label Reagent, 1 × 0.8mgTMT6-127 Label Reagent, 1 × 0.8mgTMT6-128 Label Reagent, 1 × 0.8mgTMT6-129 Label Reagent, 1 × 0.8mgTMT6-130 Label Reagent, 1 × 0.8mgTMT6-131 Label Reagent, 1 × 0.8mg90062 TMTsixplex Isobaric Label Reagent Set, sufficient reagents for 2 sixplex isobaric experiments Contents:TMT6-126 Label Reagent, 2 × 0.8mgTMT6-127 Label Reagent, 2 × 0.8mgTMT6-128 Label Reagent, 2 × 0.8mgTMT6-129 Label Reagent, 2 × 0.8mgTMT6-130 Label Reagent, 2 × 0.8mgTMT6-131 Label Reagent, 2 × 0.8mg90063TMTduplex Isobaric Mass Tagging Kit, sufficient reagents for 5 duplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mgDissolution Buffer (1 M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLTrypsin, 2 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90064TMTsixplex™ Isobaric Mass Tagging Kit, sufficient reagents for 5 sixplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mgDissolution Buffer (1M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5 M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLTrypsin, 5 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90065TMTduplex Isobaric Label Reagent Set, sufficient reagents for 5 duplex isobaric experiments Contents:TMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mg90066TMTsixplex Label Reagent Set, sufficient reagents for 5 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mg90067TMTzero™Label Reagent, 5 × 0.8mg, sufficient reagents for 5 samples90068TMTsixplex Label Reagent Set, sufficient reagents for 12 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 2 × 5mgTMT6-127 Label Reagent, 2 × 5mgTMT6-128 Label Reagent, 2 × 5mgTMT6-129 Label Reagent, 2 × 5mgTMT6-130 Label Reagent, 2 × 5mgTMT6-131 Label Reagent, 2 × 5mgStorage: Upon receipt store at -20°C. Reagents are shipped with dry ice.Note: These products are for research use only − do not use for diagnostic procedures.ContentsIntroduction ................................................................................................................................................................................. 3 Procedure Summary ..................................................................................................................................................................... 4 Important Product Information .................................................................................................................................................... 4 Additional Materials Required ..................................................................................................................................................... 4 Material Preparation .................................................................................................................................................................... 5 Preparing and Labeling Peptides with the TMT Isobaric Mass Tags .......................................................................................... 5 Preparing and Labeling Intact Proteins with the TMT Isobaric Mass Tags ................................................................................. 6 Troubleshooting ........................................................................................................................................................................... 7 Additional Information ................................................................................................................................................................ 7 Related Thermo Scientific Products ............................................................................................................................................ 9 General References . (9)IntroductionThe Thermo Scientific TMT Isobaric and Isotopic Mass Tagging Kits and Reagents enable quantitative labeling of proteins extracted from cells and tissues. Each isobaric tagging reagent within a set has the same nominal parent (precursor) mass and is composed of an amine-reactive NHS-ester group, a spacer arm and an MS/MS reporter (Figure 1). The reagents label peptides prepared from cell-based or tissue samples, either two samples for the duplex kit or six samples for the sixplex kit. For each sample, a unique reporter mass results in the MS/MS spectrum (i.e., 126-127Da for TMT 2 and 126-131Da for TMT 6 Isobaric Label Reagents). These reporter ions are in the low mass region of the MS/MS spectrum and are used to report relative protein expression levels during peptide fragmentation.The TMT Reagents share an identical structure, allowing TMTzero and TMTsixplex Reagents to be used also as isotopic “light” and “heavy” duplex tags. These tags are used to quantitate protein expression changes in cell-based or tissue samples that may not be amenable to metabolic isotopic labeling strategies (e.g., SILAC). These isotopic pairs can also be used in targeted quantitation strategies, including selective reaction monitoring (SRM, see the Additional Information Section). Peptides and proteins labeled with all TMT Reagents can be enriched with the anti-TMT Antibody Resin and TMTEnrichment Kit (see the Additional Information and Related Thermo Scientific Products Sections).Figure 1. Chemical structure of the TMT Label Reagents. Panel A: Functional regions of the reagent structure. Panel B: Structures, isotope positions, MS/MS fragmentation sites and collision-induced reporter ions for each reagent.ABProcedure SummaryProtein extracts are isolated from cells grown in culture or from tissue samples. After removing amine-based buffers and thiol reagents, samples are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed at the duplex or the sixplex level. Strong-cation exchange (SCX) fractionation simplifies complex samples before LC-MS/MS analysis. Data analysis software is used to analyze the reporter ions in the low mass region (Figure 2).Peptides are typically labeled with TMT Reagents because it allows quantitation of every peptide, but intact proteins can also be labeled. There are several advantages to labeling intact proteins. For example, combining labeled samples earlier in the sample process will reduce sample variability. Also, mixed samples enable single processing for fractionation and digestion.Fractionation methods include ion exchange chromatography, 1D-PAGE and phosphoprotein enrichment.Figure 2. Schematic for using the Thermo Scientific TMTsixplex Isobaric Mass Tagging Reagents.Important Product Information• The TMT Reagents are moisture-sensitive. To avoid moisture condensation onto the product, vial must be equilibrated to room temperature before opening.• The TMT Reagents are amine-reactive and modify lysine residues and the peptide N-termini. All amine-containing buffers and additives must be removed before digestion and labeling.• All samples must be digested, labeled and then mixed equally before desalting, fractionation and LC-MS/MS. For optimal results, use 25-100µg of peptide for each labeling reaction.• To avoid contamination of MS samples, always wear gloves when handling samples and gels. Use ultrapure MS-grade reagents. Perform sample preparation in a cleaned work area cleaned with 70% methanol (Fisher Product No. A454-1). • The TMTzero Label Reagent can be used to optimize methods before multiplexed analysis of samples with the TMTduplex or TMTsixplex Reagent Set.Additional Materials Required• Anhydrous acetonitrile (Thermo Scientific Acetonitrile HPLC grade, Product No. 51101), 100 proof ethanol or, for protein labeling, anhydrous dimethyl sulfoxide (DMSO), Sequanal grade (Product No. 20688) • Glass syringe (100µL)• HPLC grade water (Fisher, Product No. W6-4) • Chilled (-20°C) acetone• Protease inhibitors (Thermo Scientific Halt Protease Inhibitor Single-Use Cocktail, EDTA-free, Product No. 78425) • Phosphatase inhibitors (Thermo Scientific Halt Phosphatase Inhibitor Cocktail, Product No. 78420)• Cell lysis reagent such as Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Product No. 78501), RIPA Lysis and Extraction Buffer (Product No. 89901) or 8M Urea (Product No. 29700)• Protein assay such as Thermo Scientific Coomassie Plus (Bradford) Protein Assay (Product No. 23236), Pierce 660nm Protein Assay (Product No. 22600) or Pierce BCA Protein Assay Kit (Product No. 22235) • 75-300µm capillary C 18 reversed-phase column• Ion trap or time-of-flight (TOF) mass spectrometer with online or offline liquid chromatography (LC) system •Data analysis software such as Thermo Scientific Proteome Discoverer or Mascot Software (Matrix Science, Ltd.)•Optional: Thermo Scientific Zeba Spin Desalting Columns (Product No. 89882) or Slide-A-Lyzer Dialysis Cassettes,3.5K MWCO, 0.5mL (Product No. 66333)Material PreparationNote: The 50% hydroxylamine and 10% SDS stock solutions provided with the kit may precipitate during storage. Warm both solutions to room temperature and vortex before use.Albumin, Bovine (BSA) Reconstitute BSA (2.5mg) with 2.5mL of ultrapure water. Divide solution into 100µL aliquots and lyophilize to dryness.Add 50µL of the Dissolution Buffer (1M TEAB) to 450µL of ultrapure water.100mM TEAB (triethylammonium bicarbonate)2% SDS Add 50µL of the Denaturing Reagent (10% SDS) to 200µL of ultrapure water.200mM TCEP Add 70µL of the Reducing Reagent (0.5M TCEP) to 70µL of ultrapure water. Then add 35µL of the Dissolution Buffer (1M TEAB).5% Hydroxylamine Dilute the Quenching Reagent (50% hydroxylamine) 1:10 with 200mM TEAB.Preparing and Labeling Peptides with the TMT Isobaric Mass TagsA.Preparing Whole Cell Protein Extracts1.Culture cells to harvest at least 100µg of protein per condition. For best results, culture a minimum of 5 × 106 cells.2.Lyse cells in either RIPA buffer, M-PER® Reagent or 8M urea. Add protease and phosphatase inhibitors to the lysisreagent. Use 4mL of lysis reagent for each milliliter of cells.3.Perform a protein assay to determine the protein concentration. Use samples at ≥ 2mg/mL. Less concentrated samplescan be used; however, it might be necessary to use larger volumes of reducing/alkylating reagents.4.Place 100µg per condition (two for the TMTduplex or six for the TMTsixplex Label Reagents) in a polypropylenemicrocentrifuge tube.5.Add 45µL of 100mM TEAB to the sample and adjust to a final volume of 100µL with ultrapure water. For labelingreactions > 100µL use a large-volume centrifuge tube such as a 15mL or 50mL polypropylene conical tube.Optional: To solubilize complex protein mixtures, add 5µL of 2% SDS before adjusting to final volume.6.Add 5µL of the 200mM TCEP and incubate sample at 55°C for 1 hour.7.Immediately before use, dissolve one tube of iodoacetamide (9mg) with 132µL of 100mM TEAB to make375mM iodoacetamide. Protect solution from light.8.Add 5µL of the 375mM iodoacetamide (with TEAB) to the sample and incubate for 30 minutes protected from light.9.Add six volumes (~1mL) of pre-chilled (-20°C) acetone. Allow the precipitation to proceed overnight.10.Centrifuge the samples at 8000 ×g for 10 minutes at 4°C. Carefully invert the tubes to decant the acetone withoutdisturbing the white pellet. Allow the pellet to dry for 10 minutes.B.Protein DigestionNote: Use 25-100µg of purified or lyophilized protein per sample. If the protein is in solution, it must be free of amine-containing buffers. Use the BSA (100µg) as a control sample for method optimization.1.Suspend 100µg acetone-precipitated (or lyophilized) protein pellets with 100µL of 100mM TEAB.Note: An acetone-precipitated pellet might not completely dissolve; however, after proteolysis at 37°C, all the protein (peptides) will be solubilized.2.Immediately before use, add 20µL of the Trypsin Storage Solution to the bottom of the trypsin glass vial and incubate for5 minutes. Store any remaining reagent in single-use volumes at -80°C (e.g., 2.5µg of trypsin per 100µg of protein).3.Add 2.5µL of trypsin (i.e., 2.5µg) per 100µg of protein. Digest the sample overnight at 37°C.C. Peptide Labeling1.Immediately before use, equilibrate the TMT Label Reagents to room temperature. For the 0.8mg vials, add 41µL ofanhydrous acetonitrile or ethanol to each tube. For the 5mg vials, add 256µL of solvent to each tube. Allow the reagent to dissolve for 5 minutes with occasional vortexing. Briefly centrifuge the tube to gather the solution.Note: Reagents dissolved in anhydrous acetonitrile or ethanol are stable for one week when stored at -20°C and warmed to room temperature before opening.2.Carefully add 41µL of the TMT Label Reagent to each 25-100µg sample. Alternatively, transfer the reduced andaklyated protein to the TMT Reagent vial.Note: A 100µL glass syringe or positive displacement pipette may be necessary to accurately measure and dispense TMT Reagents in volatile acetonitrile solvent.3.Incubate the reaction for 1 hour at room temperature.4.Add 8µL of 5% hydroxylamine to the sample and incubate for 15 minutes to quench the reaction.bine samples at equal amounts and store at -80°C.Preparing and Labeling Intact Proteins with the TMT Isobaric Mass TagsNote: Protein labeling results in the modification of lysine residues and non-acetylated protein N-termini. Because trypsin does not recognize modified lysines, trypsin digestion cleaves only on the C-terminal side of arginine residues. The result is fewer, larger peptides and a less complex digest. Labeled proteins can be digested with other enzymes, including chymotrypsin and Glu-C.1.Solubilize and quantify protein samples as described above in Section A: Preparing Whole Cell Protein Extracts,steps 1-8.Note: If primary amine containing lysis buffers were used to prepare whole cell protein extracts, samples must be exchanged using dialysis or desalting into a suitable non-primary amine, containing buffer (e.g., TEAB, PBS, HEPES or bicine) at pH 7 to 9.2.Immediately before use, equilibrate the TMT Label Reagents to room temperature. For the 0.8mg vials, add 24µL ofanhydrous DMSO to each tube. For the 5mg vials, add 150µL DMSO to each tube. Allow the reagent to dissolve for5 minutes with occasional vortexing. Briefly centrifuge the tube to gather the solution.Note: Reagents dissolved in DMSO are stable for one week when stored at -20°C and warmed to room temperature before opening.3.Carefully add 24µL of the TMT Label Reagent to each 100µg sample. Alternatively, transfer the reduced and alkylatedprotein to TMT Reagent vial.4.Incubate the reaction for 1 hour at room temperature.5.Add 8µL of 5% hydroxylamine to the sample and incubate for 15 minutes to quench the reaction.bine samples at equal amounts. Store samples at -80°C or fractionate to remove excess tag (e.g., acetoneprecipitation, desalting or SDS-PAGE) before enzymatic digestion.TroubleshootingProblem Possible Cause SolutionPoor labeling An amine-based buffer was used Use a non-amine-based bufferIncorrect buffer pH Make sure the buffer pH is ~8.0Protein precipitation Lack of detergent present Add detergent, such as 0.05% SDS to the preparationpH decreased Make sure the pH is > 7.5Organic solvent too high For protein labeling, dissolve TMT Reagents in DMSOto minimize protein precipitationAdditional InformationA.Sample Clean-up, Enrichment and FractionationListed below are some options for peptide cleanup before MS analysis.•If SDS and DMSO were avoided during the preparation, acetonitrile may be removed by vacuum centrifugation and samples analyzed directly by LC-MS/MS. Collect MS data above 350Da to avoid signal from unincorporated tag.•SDS, solvent and unincorporated tags can be removed using TopTip™ Strong Cation-Exchange Tips (PolyLC, Product No. TT1000SEA-2003), according to the manufacturer’s instructions.•Salt and unincorporated tags can be removed using Thermo Scientific Pierce C18 Spin Columns (Product No. 89870).•Proteins labeled with TMT Reagents can be detected with the anti-TMT Antibody (Product No. 90075). Proteins and peptides labeled with TMT Reagents can be enriched with the Immobilized anti-TMT Antibody Resin and the TMT Enrichment Kit (Product No 90076 and 90077).•For best results, use an HPLC system to perform strong cation exchange fractionation to remove SDS and to fractionate complex proteomic extracts. Perform the separation with a strong-cation exchange column (PolyLC, Inc., Table 1). See also /Proteomics.v.2.htm and Trinidad, J.C. (2008).Table 1. Strong-cation exchange column information.PolyLC Part # Column Particle Pore Load Range Flow Range102SE0503 2.1 × 100 5µm 300Å 0.1-1.0mg 0.14-0.2mL/min104SE0503 4.6 × 100 5µm 300Å 0.4-4.0mg 0.7-1.0mL/minB.Data Acquisition Methods for Peptide QuantitationQuantitation of peptides labeled with Tandem Mass Tag® Reagents requires a mass spectrometer capable of MS/MS fragmentation, such as an ion trap, quadrupole time of flight, time of flight-time of flight (TOF-TOF) or triple quadrupole instrument. The choice of MS/MS fragmentation method(s) depends on the instrument capabilities such as collisionally induced dissociation (CID), pulsed-Q dissociation (PQD), higher energy collisional dissociation (HCD), or electron transfer dissociation (ETD), and the desire either to optimize one fragmentation method for both peptide identification and quantitation, or to use two methods that are each optimized for peptide identification or quantitation. For example, TMT Reagent reporter ions are not visible in ion traps following traditional CID fragmentation. Instead, quantify and identify peptides on an ion trap with PQD fragmentation or alternate PQD and CID methods optimized for identification and quantitation, respectively (Table 2). The TMT tags behave similarly to iTRAQ® Reagents (Life Technologies Corp.), although optimal chromatography and fragmentation energy settings are slightly different.Table 2. Instruments and MS/MS fragmentation options for peptide identification and quantitation with Thermo Scientific TMT Reagents.Instrument Fragmentation Method ReferenceThermo Scientific Orbitrap Velos, LTQ-Orbitrap XL, or MALDI-Orbitrap XL HCD, HCD/CID Schirle, et al. (2012), Bomgarden, etal. (2011), Lee, et al (2011), Xiong, etal. (2011), Strupat, et al. (2008)Thermo Scientific Orbitrap Velos, MS3, PTR-HCD Ting, et al. (2011), Wenger, et al.(2011)Thermo Scientific Velos Pro ion trap Trap HCD Biringer, et al. (2011)Thermo Scientific LTQ-Orbitrap Discovery or LTQ ion trap PQD, PQD/CID Bantscheff, et al. (2008), Schwartz,et al. (2008),Thermo Scientific LTQ-OrbitrapXL-ETDor LTQ-ETDETD Viner, et al. (2009) Q-TOF CID Van Ulsen, et al. (2009)TOF-TOF CID Dayon, et al. (2008)Triple Quadrupole CID, SRM Stella, et al (2011), Byers, et al.(2009)C.Data Analysis and QuantitationThe masses for peptide modification by the TMT zero, duplex, and sixplex reagents are present in the UNIMOD database () and are listed below. Several software packages directly support the modifications by TMT Reagents and the relative quantitation of reporter ions released from labeled peptides, including Thermo Scientific Proteome Discoverer (all versions) 1.1, Thermo Scientific Bioworks 3.1.1, Matrix Science Mascot 2.1 and above, and Proteome Software Scaffold Q+. For data acquired using a combination of fragmentation methods (i.e. HCD/CID or PQD/CID), Proteome Discoverer 1.1 or custom software might be necessary to merge spectra for identification and quantitation.D.Mass ModificationAll TMT Reagents share an identical chemical structure. Therefore, labeled samples behave identically during LC-MS or MALDI-MS analysis and can be quantified at either the MS/MS or MS level. For MS/MS quantitation, duplex or sixplex samples may be quantified with TMTduplex or TMTsixplex Reagent Sets. This strategy allows higher plexing and the ability to quantify specific, singly charged reporter ions without increasing sample complexity. For duplex MS quantitation, samples or internal standards labeled with TMTzero may be combined with samples labeled with a TMTsixplex Reagent, resulting in a modification of 224Da or 229Da for every labeled lysine residue, respectively. Paired peaks with a 5Da mass shift per labeled N-terminus and lysine residue are then quantified similarly to SILAC samples. This approach also may be used to quantitate specific parent and transition ions using selective reaction monitoring (SRM) strategies.Table 3. Modification masses of the Thermo Scientific TMT Label Reagents.Label ReagentModificationMass(monoisotopic)ModificationMass(average)CIDMonoisotopicReporter Mass*CID AverageReporterMass*ETDMonoisotopicReporter Mass**ETD AverageReporterMass**TMT0-126 224.152478 224.2994 126.127725 126.2193 114.127725 114.2086 TMT 2-126 225.155833 225.2921 126.127725 126.2193 114.127725 114.2086 TMT2-127 225.155833 225.2921 127.131079 127.2120 114.127725 114.2086 TMT6-126 229.162932 229.2634 126.127725 126.2193 114.127725 114.2086 TMT6-127 229.162932 229.2634 127.124760 127.2127 115.124760 115.2020TMT6-128 229.162932 229.2634 128.134433 128.2046 116.134433 116.1939 TMT6-129 229.162932 229.2634 129.131468 129.1981 117.131468 117.1874 TMT6-130 229.162932 229.2634 130.141141 130.1900 118.141141 118.1417TMT6-131 229.162932 229.2634 131.138176 131.1834 119.138176 119.1727 * CID, HCD, and PQD are collisional fragmentation methods that generate reporter ions from 126 to 131Da.**ETD is a non-ergodic fragmentation method that generates six unique reporter ions from 114 to 119Da.rmation Available from our Website•Tech Tip Protocol #49: Acetone precipitation of proteins•Tech Tip Protocol #19: Remove detergent from protein samplesRelated Thermo Scientific Products90076 Immobilized Anti-TMT Antibody Resin90075 Anti-TMT Antibody, 0.1mL88320 Pierce Peptide Retention Time Calibration Mixture, 50uL88321 Pierce Peptide Retention Time Calibration Mixture, 200uL89983 SILAC Protein Quantitation Kit – DMEM89982 SILAC Protein Quantitation Kit – RPMI 164088439 SILAC Protein Quantitation Kit - DMEM:F1287784 Pierce C18 Tips, 100µl bed, 96 tips89870 Pierce C18 Spin Columns, 25 columns28904 Trifluoroacetic Acid, Sequanal Grade23227 Pierce BCA Protein Assay23208 Pre-Diluted Protein Assay Standards88300 Fe-NTA Phosphopeptide Enrichment Kit88301 Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit90003 Pierce Phosphoprotein Isolation Kit88513 Pierce Concentrator, PES, 10K MWCO, 0.5mL89893 Zeba Spin Desalting Columns, 10mL, 5 columnsGeneral ReferencesBantscheff, M., et al. (2008). Robust and sensitive iTRAQ quantification on an LTQ Orbitrap Mass Spectrometer. Mol Cell Proteomics 7:1702-13. Biringer, R.G., et al. (2011). Quantitation of TMT-Labeled Peptides Using Higher-Energy Collisional Dissociation on the Velos Pro Ion Trap Mass Spectrometer. Application note # 520. Bomgarden, R.D., et al. (2011). Kinase Inhibitor Profiling of TrkA- and TrkB-Expressing Neuroblastoma Cell Lines Using Desthiobiotin Nucleotide Probes. Application note # 515. Byers, H.L. (2009). Candidate verification of iron-regulated Neisseria meningitidis proteins using isotopic versions of tandem mass tags (TMT) and single reaction monitoring, J Prot73(2):231-9.Dayon, L., et al. (2008). Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags. Anal Chem80(8):2921-31. Dillon, R, et al. (2011). Discovery of a Novel B-Raf Fusion Protein Related to c-Met Drug Resistance. J Proteome Res10(11):5084-94.Lee, M.V., et al. (2011). A dynamic model of proteome changes reveals new roles for transcript alteration in yeast. Mol Syst Biol7: 514.Ross, P.L., et al. (2004). Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 3(12):1154-69.Schirle, M., et al. (2012). Kinase inhibitor profiling using chemoproteomics. Methods Mol Biol795:161-77.Schwartz, J. et al. (2008). Relative quantitation of protein digests using tandem mass tags and pulsed-Q dissociation (PQD). Application note # 452.Stella, R., et al. (2011). Relative Quantification of Membrane Proteins in Wild-type and PrP-knockout Cerebellar Granule Neurons. J Proteome Res doi:10.1021/pr200759m. Strupat K., et al. (2008). Accurate MS and MS n Analysis with the Thermo Scientific MALDI LTQ Orbitrap. Application note # 30150.Ting, L., et at. (2011). MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics. Nature Methods8: 937–940.Van Ulsen, P., et al. (2009). Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. Proteomics9(7):1771-81.Viner, R.I., et al. (2009). Quantification of post-translationally modified peptides of bovine α-crystallin using tandem mass tags and electron transfer dissociation. J Proteomics72(5):874-85.Wenger, C.D., et al. (2011). Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging. Nat Methods8(11):933-5. Xiong, L., et al. (2011). Mass spectrometric studies on epigenetic interaction networks in cell differentiation. J Biol Chem 286(15):13657-68.Zhang, T., et al. (2010). Improving quantitation of TMT-labeled peptides using stepped higher-energy collisional dissociation. Application note # 483 Tandem Mass Tag and TMT are trademarks of Proteome Sciences plcTopTip is a trademark of PolyLC, Inc.iTRAQ is a trademark of Life Technologies Corp.This product (“Product”) is warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts (“Documentation”) and to be free from defects in material and workmanship. Unless otherwise expressly authorized in writing, Products are supplied for research use only. No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the original purchaser of the Product (“Buyer”).No other warranties, express or implied, are granted, including without limitation, implied warranties of merchantability, fitness for any particular purpose, or non infringement. Buyer’s exclusive remedy for non-conforming Products during the warranty period is limited to replacement of or refund for the non-conforming Product(s).There is no obligation to replace Products as the result of (i) accident, disaster or event of force majeure, (ii) misuse, fault or negligence of or by Buyer, (iii) use of the Products in a manner for which they were not designed, or (iv) improper storage and handling of the Products.Current product instructions are available at /pierce. For a faxed copy, call 800-874-3723 or contact your local distributor.© 2012 Thermo Fisher Scientific Inc. All rights reserved. Unless otherwise indicated, all trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries. Printed in the USA.。
60068标准
60068标准摘要：1.60068 标准的概述2.60068 标准的主要内容3.60068 标准的应用领域4.60068 标准的重要性正文：一、60068 标准的概述60068 标准，全称为GB/T 60068-2017《工业产品使用说明书总则》，是我国针对工业产品使用说明书制定的一项国家标准。
该标准规定了工业产品使用说明书的编制原则、内容、结构、格式和表示方法，旨在提高工业产品使用说明书的编制质量，便于用户正确、安全地操作和维护工业产品。
二、60068 标准的主要内容1.适用范围：本标准适用于工业产品的使用说明书，包括硬件产品、软件产品和系统产品等。
2.编制原则：使用说明书应遵循易于理解、内容准确、结构清晰、格式统一的原则进行编制。
3.内容要求：使用说明书应包括产品概述、安全注意事项、安装与调试、操作与控制、维护与保养、故障排除与维修、附件与工具、技术参数、性能指标等内容。
4.结构与格式：使用说明书应采用模块化结构，各部分内容应有明确的标题和序号，方便用户查找。
5.表示方法：使用说明书中的文字、图形、符号、表格、公式等应符合相关标准和规范，保证表示准确、清晰。
三、60068 标准的应用领域60068 标准广泛应用于各种工业产品，如机械设备、电气设备、仪器仪表、通信设备、计算机软件等。
在实际应用中，各类工业产品生产商应根据本标准的要求，为用户提供准确、完整的使用说明书，以提高产品的使用效果和用户体验。
四、60068 标准的重要性1.保障使用安全：60068 标准对产品使用说明书的编制提出了严格的要求，遵循这些要求可以有效地降低因操作不当导致的安全事故风险。
2.提高产品性能：准确、完整的使用说明书可以帮助用户充分发挥产品的性能，提高生产效率和产品质量。
3.提升企业形象：优质的使用说明书体现了企业的专业水平和对用户的关爱，有助于提升企业形象和市场竞争力。
4.促进行业标准化：60068 标准为工业产品使用说明书的编制提供了统一的规范，有助于推动行业的标准化、规范化发展。
TMT金海贸易公司实习操作七stu026-07sc
which the Seller reserves the right to rescind without further notice, or to regard as still valid whole or anypart of this contract not fulfilled by the Buyer, or to lodge a claim for losses thus sustained, if any.2. In case of any discrepancy in Quality/Quantity, claim should be filed by the Buyer within 130 days after thearrival of the goods at port of destination; while for quantity discrepancy, claim should be filed by the Buyerwithin 150 days after the arrival of the goods at port of destination.3. For transactions concluded on C.I.F. basis, it is understood that the insurance amount will be for 110% of theinvoice value against the risks specified in the Sales Confirmation. If additional insurance amount or coveragerequired, the Buyer must have the consent of the Seller before Shipment, and the additional premium is to beborne by the Buyer.4. The Seller shall not hold liable for non-delivery or delay in delivery of the entire lot or a portion of the goodshereunder by reason of natural disasters, war or other causes of Force Majeure, However, the Seller shallnotify the Buyer as soon as possible and furnish the Buyer within 15 days by registered airmail with a certifi-cate issued by the China Council for the Promotion of International Trade attesting such event(s).5. All deputies arising out of the performance of, or relating to this contract, shall be settled through negotiation.In case no settlement can be reached through negotiation, the case shall then be submitted to the ChinaInternational Economic and Trade Arbitration Commission for arbitration in accordance with its arbitral rules.The arbitration shall take place in Shanghai. The arbitral award is final and binding upon both parties.6. The Buyer is requested to sign and return one copy of this contract immediately after receipt of the same.Objection, if any, should be raised by the Buyer within 3 working days, otherwise it is understood that the Buyer has accepted the terms and conditions of this contract.7. Special conditions: ( These shall prevail over all printed terms in case of any conflict.)Confirmed by:THE SELLER THE BUYERGOLDEN SEA TRADING CORPORATION( signature)( signature)uyer。
tmt重捕剂反应机理_概述及解释说明
tmt重捕剂反应机理概述及解释说明1. 引言1.1 概述在有机合成领域，TMT重捕剂反应机理一直备受关注。
TMT重捕剂是一种有效的化学试剂，可用于将活性物质与靶标分子结合，并在生物医学研究中发挥重要作用。
本文旨在对TMT重捕剂反应机理进行全面概述和解释说明，以揭示其背后的原理和过程。
1.2 文章结构本文将分为五个部分来探讨TMT重捕剂反应机理。
首先，在引言部分，我们将简要介绍该主题，并明确文章的目的。
其次，在第二部分，我们将详细讨论TMT 重捕剂的定义、背景知识以及作用原理。
第三部分将解释说明TMT反应机理相关现象，包括反应速率的影响因素、产物选择性以及不同反应条件下TMT反应机理的变化等内容。
接着，在第四部分，我们将总结已有的实验成果并展望未来的研究进展方向。
最后，在第五部分中，我们将总结本文主要观点和结果，并探讨对TMT重捕剂反应机理研究的启示和意义。
1.3 目的本文的目的是全面概述和解释说明TMT重捕剂反应机理。
通过对TMT重捕剂的定义、背景知识和作用原理进行讨论，我们将揭示其在生物医学研究中的重要性。
此外，我们将分析并解释TMT反应机理相关现象，从而使读者对该主题有更深入的理解。
通过总结已有实验成果并展望未来研究方向，我们希望为该领域的科学家们提供启示和引导，并为进一步推动TMT重捕剂反应机理研究做出贡献。
2. TMT重捕剂反应机理2.1 TMT重捕剂的定义和背景知识TMT重捕剂是一种在有机合成中广泛使用的化合物，它能够促进化学反应的进行。
TMT（tetramethylthiuram）重捕剂通常是指四甲基硫代异腈（tetramethylthiuram disulfide），它作为催化剂或添加剂被广泛用于碳－有机骨架构造方法中。
在有机合成中，TMT重捕剂可以作为氧化还原催化剂、活性位点保护试剂和反应解离后续处理试剂等多种角色参与不同的反应过程。
由于其特殊的分子结构和电子亲和性，TMT重捕剂具有较好的催化活性和选择性，并且在许多重要的有机反应中表现出独特而有效的功能。
管道工程图的分类与表示方法
第一章管道工程图的分类与表示方法第一节管道工程图的分类一、按专业分按工程项目性质的不同，管道工程图可分为工业管道工程图和卫生管道（即暖卫管道）工程图两大类。
前者是为生产输送介质即为生产服务的管道，它属于工业设备安装工程。
后者是为生活或改善劳动卫生条件而输送介质的管道，它属于建筑安装工程。
本书一至四章就是讨论卫生管道工程图。
卫生管道工程又可分为建筑给水排水管道、供暖管道、燃气管道、通风与空调管道等许多具体的专业管道。
二、按图形和作用分各种管道工程施工图均可分为基本图纸和详图两大部分。
基本图纸包括图纸目录、设计施工说明、设备材料表、工艺流程图、平面图、轴测图、（立）剖面图；详图包括大样图、节点图和标准图等。
（一）图纸目录对于数量众多的施工图纸，设计人员把它按一定的图名和顺序归纳编排成图纸目录以便查阅。
通过图纸目录我们可以知道全套专业图纸的名称、图号、数量以及选用的标准图集等情况。
（二）设计施工说明凡在图样上无法表示出来而又要施工人员知道的一些技术和质量方面的要求，一般都用文字形式来加以说明。
其内容一般包括工程的主要技术参数、施工和验收要求以及注意事项。
（三）设备、材料表指该项工程所需的各种设备和各类管道、管件、阀门以及防腐、保温材料等的名称、规格、型号、数量的明细表。
以上这三点看上去不过是些文字说明，但它是施工图纸必不可少的一个组成部分，是对图形的补充和说明。
对于这些内容的了解有助于进一步看懂管道图。
（四）工艺流程图流程图是对整个管道系统一系列工艺变化过程的原理图，通过它可以对设备的编号、建（构）筑物的名称及整个系统的仪表控制点（温度、压力、流量及分析的测点）有一个全面的了解。
同时，对管道的材质、规格、编号、输送的介质、流向以及主要控制阀门等也有一个确切的了解。
（五）平面图平面图是管道施工图中最基本的一种图样，布置，管线的走向、排列和各部分的长宽尺寸，等具体数据及其相对位置。
它主要表示设备、管道在建筑物内的平面以及每根管子的坡度和坡向，管径和标高（六）轴测图（系统图）轴测图是一种立体图，又称为系统图，它能在一个图面上同时反映出管线的空间走向、帮助我们想像管线在空间的布置情况，是管道施工图中的重要图形之一。
iec60068标准
iec60068标准
IEC 60068是国际电工委员会（IEC）制定的环境试验标准，用于评估电子设备和部件在不同环境条件下的耐受性和可靠性。
该标准包含多个部分，其中最新版本是IEC 60068-1:2021。
IEC 60068标准的主要测试项目包括温度和湿度测试、振动和冲击测试、盐雾测试、尘埃测试、光照测试以及电磁兼容性测试。
这些测试旨在评估电子设备和部件在不同环境条件下的性能和耐受性，以确保其可靠性和稳定性。
此外，IEC 60068标准还包含一些相关的修订版和修正案，如IEC 60068-2-2015/AMD2:2021等，它们对于特定的测试方法和程序进行了扩展和修订，以适应新的技术和应用。
总之，IEC 60068标准是评估电子设备和部件在不同环境条件下的耐受性和可靠性的重要标准之一，对于电子产品的设计和生产具有重要意义。
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
TMT-18-6006
Temperature Compensated Amplifier
6 GHz - 18 GHz
This Amplifier offers exceptional performance over the band 6 GHz to 18 GHz with 18 dBm P1dB output power and 29 dB Gain. Teledyne Microwave amplifiers are balanced or feedback designs and manufactured with the latest MIC & MMIC technology. The GaAs FET’s used in these amplifiers are well established proven devices from multiple sources. Teledyne Microwave offers a complete line of Narrow and broadband Amplifiers covering the frequency range 500 MHz to 40 GHz. These products are available in either connectorized packages or as modules. For a complete list of these products and more, please visit our website. Other products from Teledyne Microwave Transceivers, Synthesizers, and YIG Products include:
Specifications:
Model Number: TMT-18-6006 Frequency Range: 6 GHz - 18 GHz 29 dB Gain (min): Gain Vs Temp at any Frequency: +/-1.75 dB Noise Figure (max): 5.5 dB 18 dBm Output Power @ 1dB Compression: Gain Flatness Vs Frequency: +/-2.0 dB 3rd Order Intercept (Typ): 27 dBm Power Supply: Input Voltage: 11.5 to 15 VDC 410 mA Current @ 12 VDC (typ): Current @ 12 VDC (max): 470 mA Case Type (See attached Outline): SX8 Temperature Range: -54 to +90C
TELEDYNE MICROWAVE
ISO 9001:2000
1274 Terra Bella Avenue, Mountain View, CA 94043 • PHN# 800.832.6869 or +1.650.962.6944 • FAX# 650.962.6845 • microwave@
Temperature Compensated AmpliELEDYNE MICROWAVE
ISO 9001:2000
1274 Terra Bella Avenue, Mountain View, CA 94043 • PHN# 800.832.6869 or +1.650.962.6944 • FAX# 650.962.6845 • microwave@
All Specifications are subject to change. Please contact Teledyne Microwave for the latest information on this product
08/05
元器件交易网
TMT-18-6006
All Specifications are subject to change. Please contact Teledyne Microwave for the latest information on this product
08/05