2000分子生物改版试卷B

2000级医学检验专业分子诊断学考试试卷(B卷)
______________________________________ A1型题（最佳选择题——肯定型）
每一道题有A,B,C,D,E五个备选答案，问题表述形式为肯定陈述，在答题时只需从5个备选答案中选择一个最合适的作为正确答案，并在答卷上将相应题号的相应字母画一条黑粗线。

_______________________________________ 1.What is the major characteristics of neurofibromatosis 2 ?
A.café an lait spots
B.cutaneous or subcutaneous neurofibromas
C.axillary freckling
D.acoustic neuromas and meningiomas
E. scoliosis
2.Which is required to activate LPL?
A.apoAⅠ
B.apoCⅠ
C.apoCⅡ
D.apoB48
E.apoB100
3.Which apo is CM essential?
A.apoAⅠ
B. apoAⅡ
C. apoB48
D. apoB100
E.apoCⅡ
4.In the procedure of Genomic DNA isolation, which of the following reagent serves to dissolve the cell membrane and denatures proteins?
A.cold phosphate-buffered saline
B.sodium dodecylsufate
C.phenol saturated with 0.5M Tris-HCl
D.dialysis buffer
E.chloroform
5. To evaluate if a neonate has infected HIV, which of the following methods are usually be used? A.ELISA
B.agglutilation
C.PCR
D.Wester-blot
E.immunofluorescence staining
6. Which RNA can be transferred into mRNA?
A.hnRNA
B.RNAi
C.scRNA
D.sRNA
E.snRNA
7. DNA fragment and DNA probes are to southern blotting as ( ) are to Northern blotting .
A. DNA fragments and RNA probes
B. RNA fragments and DNA probes
C.RNA fragments and RNA probes
D. protein fragments and RNA probes
E. protein fragments and DNA probes
8.Which is the smallest densist lipoproteins?
A.CM
B.VLDL
C.LDL
D.HDL
E.IDL
9.Transfer of nucleic acids by vacunm blotting is more efficient than capillary transfer and has been reported to result in a ( ) increase in final hybridization singal obtained.
A.one-to tow fold
B.tow-to three fold
C.one-to three fold
D.three-to four fole
E.tow-to four fold
10.What disorder is spinal muscular atrophy?
A.centralnervoussystemdisorder
B. autosomal-recessive disorder
C.endocrinesystemdisorder
D.autosomal-dominant disorder
E.immune system disorder
11 What is the major role of EDTA in the basic nucleic acid procedures?
A. providing a proper condition of salt for DNA to dissolve
B. denaturing proteins in the solution
C. chelating divalent cations required for nucleases
D. buffering the solution pH
E. preventing microorganism contamination
12. If the absorbance at 260nm for a DNA sample is 0.3 O.D, which of the following item correspondent to the concentration of the sample?
A. 6.9μg/ml
B.9.9μg/ml
C.11.0μg/ml
D.12.0μg/ml
E.15.0μg/ml
13. The base existing only in RNA is
A.adenine
B.guanine
C.thymine
D.cytosine
E.uracil
______________________________________ A2型题（最佳选择题——否定型）
每一道题有A,B,C,D,E五个备选答案，问题表述形式为否定陈述，在答题时只需从5个备选答案中选择一个最合适的作为正确答案，并在答卷上将相应题号的相应字母画一条黑粗线。

______________________________________ 14.Which of the clinical feature belowed is not the clinical characteristic of spial muscular atrophy?
A.anteriorhorncellsdegenerate
B.hypotonia
C.symmetricalmuscleweakness
D.wasting of voluntary muscle
E.reduced fertility 15．Which of the following is NOT correct about application of the Southern Blot?
A. It’s useful in detecting gene amplification
B. It’s useful in detecting gene mutation
C. It’s useful in detecting gene deletion
D. It’s useful in detecting gene rearrangement
E. It’s useful in detecting gene total RNA
16. Which of following is not correct about factors influencing hybridization?
A.salt concentration
B.solvent concentration
C.temperature
D. relative strength of nucleic acid hybrids
E. background signal
______________________________________填空题（每空1分。

共20分）
______________________________________ 17.The usually used molecular techniques un laboratory diagnosis of infection diseases include ,
and .
18.Key enzyme of lipoprotein catabolize have ，，.
19.The mRNA is copied beginning from it’s
-end.
20.DNA amplification cycle have three steps: denaturation, and .
21. and
probes must be visualized by exposing the hybridized membrane to X-ray film , producing an autoradiograph.
22.Rate of capillary transfer is dependent on the
of the fragments.
23.For FRAXA ,the trimucleotide repeat is localized to the region. For SBMA,HD,DRPLA,on the other hand, the repeat is within the region,and for DM, the repeat is present in the
region.
24. The relative strength of nucleic acid hybrids is
> > .
25.Poly(A)-mRNA can be isolated from total RNA by -selection.
26.Taq polymerase works best at
Centigrade.
______________________________________名词解释（每题4分，共24分）
______________________________________ 27.Souther n Blot
28.Capillary transfer
29.Taq polymerase
30.allele
31.neurofibromin
32.lipoproteins ______________________________________ E1型题（问答题，每题8分，共40分）将答案写在答题纸上，并表明题号。

______________________________________ 33. What is ISH? Please illustrate its advantage and disadvantage.
34. Please simply interpret the advantages of PCR in laboratory diagnosis of infection disease.
35. What is the strategy for molecular diagnosis of hematological malignancies?
36. What is the restriction enzyme ?
37. Please illustrate the principle and processes of PCR.。