SDS-PAGE凝胶配方

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SDS-PAGE凝胶配制试剂盒
产品简介:
碧云天生产的SDS-PAGE凝胶配制试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂,用户只需自备制胶器具和蒸馏水,即可配制PAGE胶(即聚丙烯酰胺凝胶)。

SDS-PAGE凝胶配制试剂盒不仅可用于配制SDS-PAGE凝胶,也可用于配制非变性(native)PAGE凝胶。

本试剂盒约可配制30-50块常规大小的PAGE胶。

保存条件:
1M Tris-HCl, pH8.8、10% SDS、Ammonnium persulfate (过硫酸铵)和1M Tris-HCl, pH6.8室温保存。

30% Acr-Bis (29:1)和TEMED 4℃避光保存。

过硫酸铵配制成10%溶液后,分装成小管-20℃保存,通常半年内有效。

注意事项:
过硫酸铵配制成10%溶液后,应当-20℃保存。

同时应尽量减少室温存放时间,以防失效。

TEMED易挥发,使用后请盖紧瓶盖。

另外凝胶凝聚的速度和温度及光照关系密切,可通过适当调节TEMED的用量,控制在不同的室内环境下凝胶凝聚的速度。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 根据目的蛋白的分子量大小选择合适的凝胶浓度,再按照下面的表格配制SDS-PAGE的分离胶(即下层胶):
使用本产品的文献:
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