福建三明市应用Smal-007菌剂防治松材线虫病的效果

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第54卷第1期2018年1月

业科

SCIENTIA

SILVAE

SINICAE

Vol.54,No.1

Jan.,

2018doi :10.11707/j.1001-7488.20180119

收稿日期:2016-09-05;修回日期:2017-05-27。

基金项目:国家自然科学基金项目(31170600);林业公益性行业科研专项同“重大森林病虫害防控技术关键理论基础”(201204501)。*韩正敏为通讯作者。

福建三明市应用Smal-007菌剂防治松材线虫病的效果*

1

郑催云

2

陈红梅

1

刘侃诚

2

韩正敏

2

(1.福建省三明市林业局三明350400;2.南京林业大学林学院南京210037)

要:

【目的】基于Smal-007生防菌已有防治松材线虫病的效果,选择福建省三明市林区扩大防治面积,以验

证Smal-007生防菌在我国南方林区的应用效果。【方法】选择三明市梅列区、三元区、沙县凤岗街道和沙县青州镇

4个试验点,共363.6hm 2松林进行松材线虫病防治试验。梅列区试验地103.3hm 2

,为马尾松纯林,其中防治区65.1hm 2,对照区38.2hm 2;三元区试验地100.0hm 2,多为马尾松阔叶混交林,其中防治区51.7hm 2,对照区48.3hm 2;凤岗街道试验地82.4hm 2,多为马尾松纯林,其中防治区56.5hm 2,对照区25.9hm 2;青州镇试验地77.9hm 2,多为马尾松阔叶混交林,其中防治区53.6hm 2,对照区24.3hm 2。在梅列区试验地,使用小松MD6200喷

雾机人工喷洒Smal-007菌液,喷洒量为12 15kg ·hm -2;在三元区和沙县试验地,使用海燕650-C 型轻型飞机喷洒Smal-007菌液,喷洒量为6 7kg ·hm -2。2014和2015年每年5月各喷洒1次,每年12月统计各试验地松材线虫病枯死株数,计算防治效果。【结果】人工喷洒的梅列区试验地和飞机喷洒的三元区试验地松树枯死株数明显减少,松树枯死株降低率在90%以上,其中梅列区试验地2014和2015年防治效果分别为90.9%和100.0%,三元区试验地2014和2015年的防治效果分别为93.4%和92.7%。飞机喷洒沙县试验地,由于2次喷洒后均遇到大雨,防治效果不理想,其中沙县凤岗街道试验地2014和2015年的防治效果分别为30.5%和69.4%,沙县青州镇试验地2014和2015年的防治效果分别为26.7%和20.1%。【结论】在进行Smal-007生防菌的松材线虫病防治时,只要喷洒均匀,喷洒后保持48h 无雨,就可以达到满意的防治效果,Smal-007生防菌能在闽北松材线虫病疫区推广应用。关键词:

松材线虫病;Smal-007;生防细菌;防治效果中图分类号:S763

文献标识码:A

文章编号:1001-7488(2018)01-0168-06

Control Effect of Pine Wilt Disease Using A Biological Control Agent of Smal-007in

Sanming ,Fujian Province

Yao Wu 1

Zheng Cuiyun 2

Chen Hongmei 1

Liu Kancheng 2

Han Zhengmin 2

(1.Sanming Forestry Bureau of Fufian Province

Sanming 350400;2.Forestry College ,Nanjing Forestry University

Nanjing 210037)

Abstract :

【Objective 】The strain Stenotrophomonas maltophilias Smal-007was isolated by Nanjing Forestry University

and developed as a biocontrol agent for controlling pine wilt disease.In order to verify the control effect of Smal-007in the southern forest regions of China ,a large-scale experiment was performed in forest in Sanming city ,Fujian Province.【Method 】A total of 363.6hm 2pine forests locating at four regions of Sanming city (Meilie district ,Sanyuan district ,Fenggang subdistric and Qingzhou town of Shaxian county )were used for the experiment.In Meilie district ,the experiment forest was 103.3hm 2of Pinus massoniana pure forest ,including 65.1hm 2treated with the bacterial spray and 38.2hm 2as control area.In Sanyuan district ,the experiment forest was 100.0hm 2mainly with mixed forest of P.massoniana and broad-leaved trees ,including 51.7hm 2treated area and 48.3hm 2control area.In Shaxian Fenggang ,the forest was 82.4hm 2mainly with P.massoniana pure forest ,including 56.5hm 2treated area and 25.9hm 2control area.The area of forest at Qingzhou ,Shaxian County was 77.9hm 2,mainly with P.massoniana and broad-leaved trees ,including 53.6hm 2treated area and 24.3hm 2control area.The forest in Melie district was sprayed by atomizing machine with Smal-007culture at the concentration of 12-15kg ·hm -2,while the forest in Sanyuan district and Shaxian county were sprayed by a light aircraft at a concentration of 6-7kg ·hm -2.The spraying was performed for once a year ,in May of 2014and 2015,respectively.The dead trees caused by pine wood nematode was counted from June to December every

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