细胞因子与免疫球蛋白
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II IFN IFN-
III IFN-
IFN-
CD118
CD119
T
NK
IFN-
IL-10R2
SLE
1975 Carswell
TNF 1985 Shalaby T
BCG
LPS
TNF
TNF-
(lymphotoxin,LT)
TNF-
TNF1 2
1 15min
2
ELISA
8 4ml
1 TNF-
2
TNF-
IgG
7.0-16.6g/L
macroglobulin
Ig
IgM
Ig IgM
B
mIgM
B mIgM
0.5-2.6g/L
IgA IgA
sIgA
IgD IgD :B
Ig
IgD 1%
IgD ELISA 1-60mg/L
1979
38
IL-1 ~ IL-38
cDNA
1
B
2
T
3
4
Th2 CTL
1
IL-6
2
Ag + AbAg-Ab-
Ag-Ab-
+ Ab- +
-
-Ab-Ag -Ab- - -
K2-EDTA K3-EDTA 20-25
20-25
5
2-8
1
-20
5
3
1
2
(1)
:
IL-6
CRP PCT
2 (2) IL-6
Cytokine, CK
1957 1969
1974
IFN lymphokine cytokine
(cytokinology international cytokine society
Cytokine
a.
b.
:
eg.
IL-6+ IL-6R
c.
T cell IL-2 T cell
a. b. c. d.
c.
IgM
Ig
Ig
IgG IgA IgM
g/L
Ig D IgE mg/L
20%
Ig
FcR
I
IgE IgE
1 IgE
IgE
2
IgE
IgE IgE
IgE
1.
:
2.
3.
SLE
4.
IgE
IgE
IgE IgE
(I
)
75-80%
20-23
3
3-5
IgG
Ig
IgG3>IgG1>IgG2
ADCC
SPA
MHC
a. b.
,
MHC
Lymphokine (Monokine)
interleukin, IL) interferon, IFN
tumor necrosis factor, TNF colony stimulating factor, CSF growth factor, GF
chemokine
1
G-
DIC
TNF-
2 AIDS
TNF-
3
TNF
3
LT/TNF
4
LT/TNF
1 2 3 4
1 2
3
ELISA)
RIA)
4
;
:
DNA mRNA
DNA mRNA PCR
: DNA mRNA :Southern
:Northern
PCR
PCR
RT - PCR
RT -
VS
VS
eg.
Eg. Th1/Th2
IL-2 IL-4 IL-10 IFN-
( Immunoglobulin Ig)
(Antibody Ab)
B
a. b.
1 2 3 Ab Ig Ig Ab
secreted Ig, sIg
membrane Ig , mIg
B
SIgA
a. :IgG IgD IgE
b.
IgA SIgA
a. b. c.
Activated CD4+ helper T cell Macrophage
Activated CD4+ helper T cell
Macrophage
a. b. c. d.
a. IL-4 Ig
b.
c. d.
Th2
;
IL-2 IL-4 IL-5
B
;
10-25kD pM 10-12PM
a. IL-6 CRP
b. IL-6
CRP
(
)
CRP
:
IL-6
3 (1)
a. IL-6 b. c. 4 a. PCT b.IL-6
; PCT
;
(2h
);
; :
1 2 3 IL- 6
4 IL-6 5
IL-6
IL-6
IL-6Biblioteka BaiduIL-6
IL-6
IL-6
pg/ml
<7
7-150
150-250
>250
I IFN IFNIFN-
III IFN-
IFN-
CD118
CD119
T
NK
IFN-
IL-10R2
SLE
1975 Carswell
TNF 1985 Shalaby T
BCG
LPS
TNF
TNF-
(lymphotoxin,LT)
TNF-
TNF1 2
1 15min
2
ELISA
8 4ml
1 TNF-
2
TNF-
IgG
7.0-16.6g/L
macroglobulin
Ig
IgM
Ig IgM
B
mIgM
B mIgM
0.5-2.6g/L
IgA IgA
sIgA
IgD IgD :B
Ig
IgD 1%
IgD ELISA 1-60mg/L
1979
38
IL-1 ~ IL-38
cDNA
1
B
2
T
3
4
Th2 CTL
1
IL-6
2
Ag + AbAg-Ab-
Ag-Ab-
+ Ab- +
-
-Ab-Ag -Ab- - -
K2-EDTA K3-EDTA 20-25
20-25
5
2-8
1
-20
5
3
1
2
(1)
:
IL-6
CRP PCT
2 (2) IL-6
Cytokine, CK
1957 1969
1974
IFN lymphokine cytokine
(cytokinology international cytokine society
Cytokine
a.
b.
:
eg.
IL-6+ IL-6R
c.
T cell IL-2 T cell
a. b. c. d.
c.
IgM
Ig
Ig
IgG IgA IgM
g/L
Ig D IgE mg/L
20%
Ig
FcR
I
IgE IgE
1 IgE
IgE
2
IgE
IgE IgE
IgE
1.
:
2.
3.
SLE
4.
IgE
IgE
IgE IgE
(I
)
75-80%
20-23
3
3-5
IgG
Ig
IgG3>IgG1>IgG2
ADCC
SPA
MHC
a. b.
,
MHC
Lymphokine (Monokine)
interleukin, IL) interferon, IFN
tumor necrosis factor, TNF colony stimulating factor, CSF growth factor, GF
chemokine
1
G-
DIC
TNF-
2 AIDS
TNF-
3
TNF
3
LT/TNF
4
LT/TNF
1 2 3 4
1 2
3
ELISA)
RIA)
4
;
:
DNA mRNA
DNA mRNA PCR
: DNA mRNA :Southern
:Northern
PCR
PCR
RT - PCR
RT -
VS
VS
eg.
Eg. Th1/Th2
IL-2 IL-4 IL-10 IFN-
( Immunoglobulin Ig)
(Antibody Ab)
B
a. b.
1 2 3 Ab Ig Ig Ab
secreted Ig, sIg
membrane Ig , mIg
B
SIgA
a. :IgG IgD IgE
b.
IgA SIgA
a. b. c.
Activated CD4+ helper T cell Macrophage
Activated CD4+ helper T cell
Macrophage
a. b. c. d.
a. IL-4 Ig
b.
c. d.
Th2
;
IL-2 IL-4 IL-5
B
;
10-25kD pM 10-12PM
a. IL-6 CRP
b. IL-6
CRP
(
)
CRP
:
IL-6
3 (1)
a. IL-6 b. c. 4 a. PCT b.IL-6
; PCT
;
(2h
);
; :
1 2 3 IL- 6
4 IL-6 5
IL-6
IL-6
IL-6Biblioteka BaiduIL-6
IL-6
IL-6
pg/ml
<7
7-150
150-250
>250
I IFN IFNIFN-