血管内皮生长因子对脂多糖导致的小鼠早期肺炎性反应的影响

血管内皮生长因子对脂多糖导致的小鼠早期肺炎性反应的影响龚红月;冯得胜;曾祥刚
【期刊名称】《四川医学》
【年(卷),期】2015(000)009
【摘要】目的：观察血管内皮生长因子( VEGF)对脂多糖导致的小鼠早期急性肺损伤的影响。

方法45只雄性C57BL6小鼠随机分为生理盐水对照组(C组)、脂多糖组(L组)与血管内皮生长因子干预组(L+V组)，每组各15只。

脂多糖组和干预组采用腹腔注射脂多糖(10 mg/kg)建立急性肺损伤模型，干预组在脂多糖注射后经尾静脉一次性注射血管内皮生长因子4ng。

造模6h后取血行血气和血常规分析，取肺组织行HE染色观查病理学改变，并且用ELISA法检测肺组织及血浆中炎性因子TNF-α及IL-6的表达情况。

结果 L组在注射脂多糖之后外周血白细胞总数和血小板数量降低，乳酸值及碱剩余值明显升高，肺组织炎性损伤明显，肺组织及血浆中IL-6及TNF-α水平明显增加；L+V组较L组外周血白细胞总数进一步降低，乳酸值及碱剩余值进一步升高，肺组织炎性损伤加重，肺组织IL-6及TNF-α水平进一步增加。

结论血管内皮生长因子能显著加重脂多糖导致的早期肺炎性反
应。

%Objective To study the effect of vascular endothelial growth
factor( VEGF) on lipopolysaccharide( LPS)-in-duced acute lung
injury(ALI)mouse. Methods A total of 45 male C57BL6 mouse were randomly divided into three groups:NS control group(C group,n=15),LPS group(L group,n=15)and VEGF intervention group(L+V group,n=15). ALI model was developed by injecting LPS intraperitoneally 10mg/kg in LPS group and VEGF intervention group. The VEGF intervention group was
injected VEGF(4ng)one-time via tail vein after LPS injection. After 6h of model,the arterial blood sample were collected to a-nalysis blood gas and blood routine. Then pulmonary histological changes were evaluated by hematoxylin-eosin stain;the expression of TNF-αand IL-6 in lung tissue homogenates and plasma were measured by ELISA. Results In ALI group,the total leukocytes and blood platelet count decreased,but the value of clac and BE increased significantly. The lung pathological section showed apparently inflammatory injury. The level of TNF-αand IL-6 in lung tissue and plasma markedly increased. In L+V group,the total leukocytes and blood platelet count further decreased and the value of clac and BE further increased than L group. The lung inflammatory injury was more serious compared with L group. The level of TNF-α and IL-6 in lung tissue were higher in L+V group compared with L group. Conclusion VEGF could significantly aggravate the inflammatory reaction in early stage of Lipopolysaccharide(LPS)-in-duced acute lung injury.
【总页数】5页(P1264-1268)
【作者】龚红月;冯得胜;曾祥刚
【作者单位】仁寿县人民医院麻醉科，胸外科，四川仁寿620500;仁寿县人民医院麻醉科，胸外科，四川仁寿620500;贵阳医学院附属医院麻醉科，贵州贵阳550004
【正文语种】中文
【中图分类】R563
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