pET28a-mCherry使用说明

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WPM28 Series 专业弹粟机器使用说明说明书

WPM28 SeriesPROFESSIONAL POPCORN MAKERFor your safety and continued enjoyment of this product,always read the instruction book carefully before using.CONTENTSImportant Safeguards (2)Power Cord Instructions (4)Parts (5)Before First Use (6)Assembly Procedure (6)Popping Instructions (8)Cleaning & Maintenance (9)IMPORTANT SAFEGUARDS When using any electrical appliance, basic safety precautions should always be taken, including the following:1. R EAD ALL INSTRUCTIONS BEFOREUSING YOUR POPCORN MAKER.2. U NPLUG MAIN POWER CORD FROM OUTLET WHILENOT IN USE AND BEFORE CLEANING.3. T he use of accessory attachments not recommendedby Waring may cause injuries.4. K EEP OUT OF REACH OF CHILDREN. Close supervisionis necessary when this appliance is used by or nearchildren.5. D o not allow cord to hang over any edge where a childcan reach it. Arrange cord to avoid pulling, tripping orentanglement.6. T o avoid any risk of electrical shock, do not immerse cordor plug into water or other liquids.7. D o not operate the appliance with damaged cord or plug,or after the appliance malfunctions or has been damagedin any matter. Send the unit to the nearest authorizedservice center for examination, repair or adjustment.(ONLY technicians should open up the unit.)8. E xtreme caution must be used when moving an appliancecontaining hot oil or other liquids. Allow hot parts to cooldown before handling or moving the appliance.9. Do not attempt to repair this appliance yourself.10. Do not attempt to pop corn kernels without using oil.11. Do not immerse popping kettle in water.12. K ettle surface is HOT and should NOT be touched duringpopping or just after use.13. D o not touch hot surfaces. Always use handles or knobs tooperate.14. T his appliance should not be used by or near children orindividuals with certain disabilities.15. A llow HOT parts to cool down before maneuvering andcleaning.16. T urn ALL switches to the OFF position beforedisconnecting power plug from the wall supply outlet.17. Not recommended for outdoor use.18. Do not use appliance for other than intended use.19. T o avoid overheating, always add oil and then kernelsbefore operating.20. E xtreme caution must be used when unloading popcorn.Always place the unit over a countertop so that residual hot oil or water running out will not cause burns.21. DO NOT CLEAN USING ANY ABRASIVE MATERIAL.22. N EVER FORCE ANY PARTS OR BRACKETS INTOPOSITION.23. C AUTION: a burn can result from misuse of this product.Read popping instructions for proper procedure.24. No parts are intended for the dishwasher.25. I f plugged into an outlet, the unit should not be leftunattended.26. D o not let cord hang over edge of table or counter,or touch hot surfaces.27. D o not place on or near a hot gas or electric burner,or in a heated oven.26. D o not operate your appliance in an appliance garageor under a wall cabinet. When storing in an appliancegarage always unplug the unit from the electricaloutlet. Not doing so could create a risk of fire, especially if the appliance touches the walls of the garage or the doortouches the unit as it closes.SAVE THESE INSTRUCTIONS FOR HOUSEHOLD USE ONLYPOWER CORD INSTRUCTIONS1. A short power supply cord is provided to reduce the riskresulting from becoming entangled in or tripping over alonger cord.2. L onger, detachable power supply cords or extension cordsmay be used if care is exercised in their use.3. I f a long detachable power supply cord or extension cord isused:a. T he marked electrical rating of an extension cordshould be at least as great as the electrical rating of theappliance.b. I f the appliance is of the grounded type, the extensioncord should be a grounding 3-wire cord.c. T he longer cord should be arranged so that it will notdrape over the countertop or tabletop where it can bepulled on by children or tripped over.This appliance is for 120 volts only and should not be used with a converter.PARTS1. Housing2. ON/OFF Switch3. Kettle4. Kettle Handle5. Internal Kettle Plug6. Kettle Lid7. Kettle Lid Handle8. Kettle Stirrer9. Door (removable)10. Tilt Door (removable)11. U npopped KernelCatcher & Screen12. Kettle Release Tab13. Kettle Hook14.A ccessories– Oil Measuring Spoon – 1 tbsp.– Kernel Measuring Cup – ¼ cup– Popcorn Scoop15. Main Power Cord (not shown)4BEFORE FIRST USEMake sure the popcorn maker is unplugged before you perform the following:1. C heck to be sure the voltage (120V – 60 Hz) is suitable toyour home.2. C lean the internal surface of the kettle with warm soapywater; rinse well and dry with a clean cloth. Wipe downinner and outer housing with a damp cloth. Be sure that all parts are completely dry before assembly.ASSEMBLY PROCEDURE1. R2. P3. I4. I5. Attach tilt door.6. Place door on hinges.POPPING INSTRUCTIONS1. B e sure that the popcorn maker has been assembledproperly before plugging in and popping.2. Plug the main power cord into an electrical outlet.3. A dd 2 tablespoons of oil (vegetable, canola, corn, peanutor safflower oil) and ½ cup of corn kernels to the kettle;lower the lid.N ote: Use 2 scoops of the oil measuring spoon providedand 2 scoops of the kernel measuring cup provided.4. Turn the power switch to the ON position.N ote: You do not need to allow the kettle to heat upbeforehand.5. A fter approximately 3 minutes, popcorn will start to pop upand out of the lid.6. W hen the unit is finished popping – you will no longer hearthe corn popping – immediately turn the power switch tothe OFF position and press down on the kettle release tab located on the right of the housing to release the kettle.This will allow the excess popcorn to fall from the kettle. 7. B efore scooping popcorn, return the kettle to the uprightposition by using the kettle handle. CAUTION: KETTLEIS HOT.8. U se the popcorn scoop to remove the popcorn fromthe unit.9. A fter the popcorn has been removed from the unit, removethe screen of the kernel catcher at the bottom of the unit.Remove the tray and discard the unpopped kernels.10. R emove plug from the power supply if the unit will not beused for additional batches.11. Repeat steps 3 through 9 for additional batches.CLEANING & MAINTENANCEAfter using the popcorn maker, let it cool completely. Use a wet cloth to clean the housing and doors.It is strongly recommended to clean the kettle frequently to avoid burning excess oil onto the bottom of the inner pot.1. Turn the power switch to the OFF position.2. P our 4 to 6 ounces of water (NO detergent is necessary)into the kettle.3. T urn the power switch to the ON position to boil the water,allowing the stirrer to remove excess oil stuck on thesurface.4. A fter about 1 to 2 minutes of boiling, allow to cool, thenpour water into a deep bowl and discard. CAUTION: THEKETTLE IS HOTYou may also remove the kettle and clean it with a non-scratch scouring pad. Do not immerse kettle or electrical plug in water.Any other servicing should be performed by an Authorized Service Representative.NOTES_______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ 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_______________________________________________________________________________________ _______________________________________________________________________________________NOTES_______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ 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_______________________________________________________________________________________ _______________________________________________________________________________________11Trademarks or service marks of third parties used herein are the trademarks or service marks of their respective owners.©2014 Waring Consumer Products150 Milford RoadEast Windsor, NJ 08520Printed in ChinaWPM28 IB14WR004325。

所有质粒载体汇总

酿酒酵母表达载体pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重组表达载体pUG6, pSH47,酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187, 酿酒酵母AH109；质粒pGADT7,pGBKT7；对照质粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，酿酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190,毕赤酵母表达载体pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套毕赤酵母Pichiapink,毕赤酵母宿主X33，KM71，KM71H，GS115，原核表达载体pQE30,31,32,40,60,61,62,等原核表达载体,包括pET系列，pET-GST，pGEX系列（含GST标签），pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pQE系列,pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA （分泌型表达系列）,pQBI63（原核表达带荧光）pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF（+）, pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK （+）,pBlueScript SK（-）pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大肠杆菌冷激质粒： pColdI pColdII pColdIII pColdTF 原核共表达质粒：..pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴侣： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞： DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3）HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI）BL21（DE3）plysS TG1 TB1 DH5a（pir）Tuner（DE3）Bl21 codonplusRIPL Novablue （DE3）Rosetta Rosetta（DE3）Rosetta（DE3）plys Rosetta-gami（DE3）Rosetta-gamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami（DE3）OrgamiB（DE3）HMS174（DE3）植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIAsuper1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2,T-DNA插入载体（随机突变体库）pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1枯草芽孢杆菌表达载体pWB980,pHT43,pHP13,pHP43,pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,pAL12,pUCX05-bgaB,pHT01，配套菌株BS 168,WB600,WB800,WB700,WB800N,1012,FZB42,1A747,广宿主质粒pVLT33RNAi基因沉默干扰敲除载体pSilencer1.0，pSilencer 2.1-U6 hygro， pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo， pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi载体（oligoengine）pSuper-puro RNAi逆转录病毒载体（clontech）: RNAi-Ready pSIREN-Retro Q， RNAi-ReadypSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide）RNAi慢病毒载体（addgene）: pLKO.1..哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1（-）/myc-His A ，pcDNA6-Myc/His B，pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay, 四环素调控系统（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四环素调控系统（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFA T-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY3 00PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosusGG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444（GFP）, pHY300PLK,pRT102,pRL1063a, 转座子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152,pOJ260,pPG611.1,pPG612.1，..腺病毒载体/慢病毒，逆转录病毒表达载体及包装包膜质粒，腺病毒系统（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ,pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大肠杆菌BJ5183,293,293T cellline 腺相关病毒系统（Stratagene）： pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS，慢病毒载体:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v 5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q,RNAi-Ready pSIREN-Retro Q-ZsGreen,pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry质粒载体。

Cherry 工具手册2009

我们的工具，你们的效益
60多年以来，全世界的飞机制造公司和维修公司广泛使用我们的工具。每个紧固系统都配有一整套工具，来提供一套系统的方法，保证最佳的兼容性和一致的安装性能。
我们的工具在世界范围被广泛使用，所有工具都经过了CE的认证，可提供多种语言的使用手册。我们在全球拥有大量的销售人员，可以在用户现场、培训中心等地进行紧固件和工具的操作培训。我们的技术服务部门的电话是714-545-5511，可以解决各种产品相关问题，回答车间用户、工程师和经销商提出的技术问题。
第5节转接器和附件
........................................................................................................................................ 33
第6节紧固件零件号和工具交叉对照 CherryMAX® 抽钉零件号和工具选用指南 NAS9301B-NAS9312MP 或 CR3213-CR3555P ............................................................ 35 CherryMAX® ‘AB’ 抽钉零件号和工具选用指南 NAS1399D( )AB( )-NAS1398CW( )AB( ) 或 CR4172-CR4623CW ................................... 36 SST® 抽钉零件号和工具选用指南 CR6222-CR6253 ............................................................................................................. 37 CherryLOCK® ‘A’ 抽钉零件号和工具选用指南 NAS1399D( )A( )-NAS1398CS( ) 或 CR2172-CR2673 ..................................................... 38 CherryLOCK® 抽钉零件号和工具选用指南 NAS1399D-NAS1398CS 或 CR2162-CR2663S ............................................................. 39 Maxibolt™ 抽钉零件号和工具选用指南 MS90353S-MS21141S 或 CR7310S-CR7774S ............................................................ 40 环槽钉零件号和工具选用指南

CHERRY MV 3.0键盘说明书

Mechanical keyboard with CHERRY Viola switchesModels may vary from the image shownYour keyboard – Your style.Make a statement with this brilliantly illuminated corded mechanical keyboard designed with CHERRY Viola switches. A new typing experience for work or play.The CHERRY MV 3.0 mechanical keyboard is built to perform. Designed with a rigid and durable aluminum surface for comfortable typing, passive surrounding light ring, and CHERRY Viola RGB mechanical keys. Make a dynamic statement on your desk.Expand beyond the preloaded dynamic lighting effects with per key color customization provided through CHERRY U TILITY software, or program individual keys to create custom macros - open programs or files.The CHERRY Viola key switch is teamed with comfortably spaced keycaps that help you glide through your day or night. The two-stage, progressive force - cross linear switch offers a fast reset and an exceptionally smooth slide – a much improved type feel over rubber dome keyboards.Make no compromise with the professional and gaming features like WIN key lockout, anti-ghosting, Full N-Key R ollover, and detachable USB-C cable - everything you need to perform. Key benefits→S triking keyboard with brightly lit aluminum surface and mechanical keys→Impressive RGB lighting in more than 16 million colors, with numerous integrated color routines→CHERRY key for instantly accessing the CHERRY UTILITY software to customize keyboard settings such as keyand light effects→Mechanical CHERRY V iola switches with self-cleaning V-shape contact system and two-stage, linear feel –developed and made in Germany→Full N-K ey R ollover – all keys are read simultaneously →Anti-ghosting – no input errors→WIN key lockout→Abrasion-resistant key caps→Detachable USB-C cable→Folding feetMechanical keyboard with CHERRY Viola switchesTechnical DataLayout (country or language)Product dependant, see table "Models"Housing color (product dependent)black + grey-aluminiumKey color (product dependant)white or blackWeight (product)Keyboard: 31.6 oz.Total weight (with packaging)approx. 40.7 oz.Cable Lengthapprox. 5.25 ft.Storage Temperature-4 °F to 140 °FOperating Temperature32 °F to 122 °FCurrent Consumptionmax. 500 mAInterfaceUSB-CProduct compliance:•CE•FCC•China RoHS•KCC•BSMI•WHQLSystem requirementsUSB-A ConnectionDelivery Volume•CHERRY Keyboard•Detachable USB-C cable•Printed manualDimensionKeyboard: approx. 17.12 x 5.31 x 1.18 in. Packaging: approx. 17.91 x 7.48 x 1.57 in.DatasheetCHERRY MV 3.0Mechanical keyboard with CHERRY Viola switches Keyboard•Key technology: CHERRY Viola, mechanical•Service life and characteristics depend on switch specification (see ) •Inscription technology: lasered•Number of Keys: Product dependant, see table "Models" •Number of additional keys: 4 + CHERRY KeyPalm rest-Packaging unitNumber of products in the master package: 8 Warranty2 yearsErrors, technical changes and delivery possibilities excepted. Technical information refers only to the specifications of the products. Features may differ from the information provided.CHERRY MV 3.0Mechanical keyboard with CHERRY Viola switches©CherryAmericas•573295thAvenue,Suite850•Kenosha,WI53144•USAPhone:262.942.6508•**********************************••2021-12-14Models(possible country/layout versions, others available on request)Product name Light Product No. (P/N) Mode No. (M/N) IndexEAN-CodeLayoutColorSwitchKeycapsMV 3.0 RGB G8B-26000LYAEU-0 G8B-26000 00 4025112094330 US-international white MV red lasered MV 3.0 RGBG8B-26000LYAEU-2G8B-26000004025112094347US-internationalblackMV redlasered。

H28抽濕機操作說明書说明书

請在操作之前務必認真閱讀本手冊，並妥善保管作為日後使用時參考！產品設計與規格會因改良作出變更，恕不另行通知。

請向銷售代理或是製造商諮詢，了解細節。

Model：H28抽濕機使用說明書 (1)Before operating this product, please read the instructions carefully and save this manual for future use!Operation manual (13)DehumidifierThe design and specifications are subject to change without prior notice for product improvement. Please with the sales agency or manufacturer for details.目錄使用此抽濕機的時候，必須遵守下列資訊:廢棄: 廢棄本產品時，不可當作未分類的城市廢棄物處理。

需要特別處理時，請分別集中此類廢棄物。

嚴禁以家用廢棄物名義廢棄此設備。

若需廢棄此產品，有幾種方式可選擇:A) 至政府已經訂定集中系統，至少可於此處以使用者免費的方式廢棄電子廢棄物。

B) 購買新產品時，經銷商至少會以免費方式回收舊產品。

C) 製造商至少會以使用者免費的方式回收舊的產品。

D) 當舊產品內含有價資源時，可出售給廢金屬收購商。

若是任意丟棄於森林與環境中，危險物質外洩至地下水並進入食物鏈中時會危害您的健康。

溫馨提示安全提示警告 ..............................................................................................................................2-3注意 ...................................................................................................................................3電子信息 .........................................................................................................................4控制面版介紹按鍵功能..........................................................................................................................5其他功能...........................................................................................................................6產品部件介紹產品部件描述..................................................................................................................7機器的擺放........................................................................................................................8產品操作機器的使用.......................................................................................................................8清理回收水.......................................................................................................................9清潔及保養.....................................................................................................................10常見問題解答..................................................................................................................11詳閱本手冊注意在本手冊中，您將可找到許多有用的提示，提醒您如何正確使用以及維護您的抽濕裝置。

PO-28机器人用户指南说明书

folding standpatternto select pattern hold pattern and press 1-16bpmpress to select tempomhold to set master volume 1-16hold step 1-16 + mto set multipliersoundto select sound hold sound and press 1-16alarm selected tempoPO-28 robot manualsound 1-16e ect 1-161. distorted ﬁlter2. distorted sweep3. repeat 44. repeat 25. lowpass slow sweep6. lowpass fast sweep7. hipass ﬁlter8. hipass sweep9. ﬁll-in10. ﬁll-in (fast)11. retrigger12. retrigger (fast)13. glitch14. glitch (fast)15. blinds16. noise e ect1. LFO pulse wave2. pulse wave3. mixed wave4. triangle wave5. echo6. arpeggio7. vibrato8. 4-bit triangle9. LFO pulse wave 10. pulse wave 11. mixed wave 12. triangle wave 13. echo 14. arpeggio 15. vibrato16. micro drum kitsound key 16 is a micro drum machinethe unit auto powers o when no buttons are pressed for a while, only powering the display to show time.press sound + bpm to show battery status. press sound + write to manually power o . this ensures that recent modiﬁcationsto pattern data will survive a loss of power.sound 1-16e ect 1-161. getting startedpower-upinsert two fresh AAA batteries.pay attention to plus and minus terminals.set clockset time by turning knob A for hours and knob B for minutes. press any key to conﬁrm and exit. alarm clockpress sound + pattern. set alarm clock by turning A for hours and B for minutes. (disable by turning knob A all the way down.) press any key 1-16 to set pattern for the alarm. to stop alarm press any key. reset clockreset the clock by removing the batteries and start over.2. live playselect soundhold sound and press any key 1-8 to select a sound in the high-octave range. hold sound and press any key 9-15 to select a sound in the low-octave range.play melodiespress keys 1-16 to play notes from a ﬁxed scale.tweak the soundknob A controls the timbre of the current sound. knob B controls the release rate.auto vibratohold glide and tweak knobs to control automatic vibrato. vibrato is applied a short while after the onset of every live note. knob A controls vibrato depth, and knob B controls the rate. to turn o this feature, turn knob A all the way to the left.add expressionhold glide and press any key 1-16 to glide up to a note.hold glide while releasing a note key to activate a falling chromatic scale e ect.micro drum kithold sound and press 16 to select the micro drum kit.press keys 1-16 to trigger an assortment of 8-bit drum sounds.3. sequencerthe device is equipped with a16-step, 2-channel sequencer. the ﬁrst sequencer channel is for melodies, and the second is for drums.playbackhold pattern and press any key1-16 to select pattern. blinking led indicates active pattern. press play to start and stop playback. making a beatpress write (rec symbol appears) to edit the contents of a pattern. enter notes/sounds in the grid. active steps will be lit. press play to listen to your pattern. when sound 16 is selected, the grid re-presents the drum channel. whenany other sound is selected, thegrid represents the melody channel.select soundwhile write mode is active, holdsound and press any key 1-8 (or9-15) to select a sound for themelody channel.parameter lockingduring play, hold write whileturning knobs to write soundparameter changes over time.set note / drumwhile write mode is active, hold alit step and turn knob A to set notevalue or to select drum sound.ﬁne-tuning the patternwhile write mode is active, hold alit step and turn knob B to modifythe release rate on that step.hold a lit step and press glide totoggle the glide-up e ect on thatstep. hold an unlit step and pressglide to toggle the glide-downe ect on that step.4. e ectswhile playing, hold FX and pressany key 1-16 to punch in e ects.hold FX without pressing any keyto clear e ects. see reference onopposite page. the ﬁlter e ectsalso work in live mode when thesequencer is stopped. hold FX andpress the corresponding key toactivate the e ect. press FX brieﬂyto deactivate it.5. tempo and swingpress bpm to switch tempo. thebpm will be displayed in the upperright corner of the screen.HIP HOP (80 bpm)DISCO (120 bpm)TECHNO (140 bpm)hold bpm and turn A to adjustthe swing. hold bpm and turn B toﬁne-tune tempo from 60 to 240 bpm.6. volumehold bpm and press any key 1-16to adjust master volume.7. live recordwhile playing, except in writemode, hold write and press anykey 1-16 to punch in notes.notes will be quantized accordingto the current swing setting.8. step multiplierin write mode, ensure that themicro drum kit (sound 16) isselected, so that the gridrepresents the drum channel.hold a lit step, and press bpmto insert a re-trig on that step.press bpm multiple times to switchbetween 2, 3, 4, 6 or 8 multiplier.9. making a songcopy patternhold write + pattern and press1-16 to paste the active pattern tothe corresponding new slot.clear patternhold glide and press pattern toclear the active pattern.chain patternhold pattern and press a sequenceof keys 1-16 to create a chain ofup to 128 patterns. one patterncan be selected multiple times.example: 1, 1, 1, 4 plays pattern 1three times then moves on to pattern4. the entire sequence then repeats.11. sync multiple unitsconnect a standard stereo audiocable between the units. themaster unit will control the tempoof the slave unit. hold glide andpress bpm on master unit totoggle sync modes. pressrepeatedly to toggle betweendi erent modes displayed in theupper right corner of the screen.press play on slave unit to wait formaster clock sync. press play onmaster to start.sync modesthere are 5 sync modes. defaultmode is SY0. when sync is usedthe signal will be split betweenaudio (right) and sync (left).sync scenariosexample Achain: PO-28 PO-20 PO-12example Bchain: external PO-28SY2iphonecomputersynckontrolexample Cchain: ext. PO-28 PO-14setting:example Dchain: PO-28setting:disclaimeralthough teenage engineering will use all reasonableendeavours to ensure the accuracy and reliability ofthis product, neither teenage engineering nor anythird party supplier will be liable for any loss ordamage in connection with the sale or use of theproduct except for: a) personal injury caused by ournegligence or that of our employees or agents whenacting in the course of their employment withourselves and; b) any other direct loss or damagecaused by our gross negligence or wilfulmisconduct.ALL EXPRESS OR IMPLIED CONDITIONS,WARRANTIES OR UNDERTAKINGS (OTHER THANCONDITIONS, WARRANTIES OR UNDERTAKINGSEXPRESSLY STATED, OR IMPLIED BY STATUTEAND WHICH CANNOT BE EXCLUDED), WHETHERORAL OR IN WRITING, INCLUDING WARRANTIESAS TO SATISFACTORY QUALITY AND FITNESS FORA PARTICULAR PURPOSE, ARE EXCLUDED.©2016 teenage engineering ab.all rights reserved.。

晶玛电子产品说明书

1||2P H O T O V O L T A I C F U S EL I N K SF U S E HO L D E R S & B A S E S P A G E 02 | g P V 10x 38 & 14x 51 600 &1000V /1100 D C F U S E L I N K S P A GE 05 | P M X 10x 38 & 14x 51 1000V D CF U S E H O L D E R S P A GE 06 | P M L 10/14x 85 1500V D C F U S E H O L D E R S P A GE 08 | C L I P C O N T A C TF O R φ10 F U S E L I N K S P A GE09|S T N H 1, N H 2 & N H 3 1000V D C F U S E B A S E S P A G E 03 | g P V 10x 85 1200 & 1500V D C F U S E L I N K S P A GE 04| g P V N H 1, N H 2 & N H 3 1000V D C F U S E L I N K S|2gPVFUSE-LINKS FOR PHOTOVOLTAIC APPLICATIONSPV fuse-links for photovoltaic installations from DF Electric have been developed to offer a compact, safety and economic protection so-lution in photovoltaic installations where, due to the increase of the power and technologic evolution, no-load voltages above 800V DC are reached. Also meet the requirements for instruments (multimeters) and traction equipment auxiliary circuits. The range comprises 10x38 & 14x51 fuse-links with rated currents between 1A and 20A. Rated voltage is 600 V DC or 1000V DC (direct current). Provide protection against overloads as well as short-circuits. Made with ceramic tube with high withstand to internal pressure and thermal shock, that allowsa high breaking capacity in a reduced physical space. Contacts are made of silver plated copper and melting elements are made of pure silverin order to avoid the aging and thus keep unalterable the electric characteristics. For these fuse-links we recommend the utilization of PMX 1000V fuse holders in single pole version or two-pole version.I n REFERENCE BREAKING CAPACITY PACKING(A)(kA)Uni./BOXFUSE-LINKS PHOTOVOLTAICCOMPATIBLEPV FUSE HOLDERS FORPHOTOVOLTAICAPPLICATIONSPAGE05COMPATIBLECLIP CONTACT FOR φ10FUSE-LINKSPAGE07TECHNICALt-I CHARACTERISTICSPAGE09STANDARDSIEC 60269-1IEC 60269-6UL 2579TECHNICALAMBIENT TEMPERATUREDERATING FACTORPAGE12AØBC20492235104/2425492240104/242492202104/244492205104/246492210104/248492215104/2410492220104/2412492225104/2415492229104/2416492230104/24STANDARDSIEC 60269-1IEC 60269-6UL 2579FUSE-LINKS PHOTOVOLTAICI n REFERENCE BREAKING CAPACITY PACKING(A)(kA)Uni./BOXCOMPATIBLECLIP CONTACT FOR φ10FUSE-LINKSPAGE07TECHNICALt-I CHARACTERISTICSPAGE10TECHNICALAMBIENT TEMPERATUREDERATING FACTORPAGE123||4gPV NH 373245NH1NH3NH2373350FUSE-LINKS FOR PHOTOVOLTAIC APPLICATIONSFUSE-LINKS FOR PHOTOVOLTAIC APPLICATIONSNH gPV 1000V DC fuse-links for photovoltaic installations have been developed to offer a compact, safety and economic pro-tection solution in second level panels of photovoltaic installations. The range comprises NH1 fuse-links with rated currentsbetween 25A and 200A, NH2 with rated currents between 200A and 250A and NH3 fuse-links with rated currents between200A and 400 A. Rated voltage is 1000V DC (direct current). They provide protection against overloads as well as short-circuits (gPV class according to IEC 60269 Standard), with a minimum fusing current of 1,35·In. Made with ceramic body with highwithstand to internal pressure and thermal shock. Contacts are made of silver plated copper or brass and melting elements are made of pure silver in order to avoid the aging and thus keep unalterable the electric characteristics. For these fuse-links werecommend the utilization of 1000V NH ST fuse bases.COMPATIBLENH FUSE BASES FOR PHOTOVOLTAIC APPLICATIONSPAGE08TECHNICALt-I CHARACTERISTICSPAGE11TECHNICALAMBIENT TEMPERATURE DERATING FACTORPAGE12STANDARDS IEC 60269-1IEC 60269-6UL 2579I nREFERENCEBREAKING CAPACITYPACKING(A)(kA)Uni./BOX5|485152PHOTOVOLTAICFUSE HOLDERS FOR PHOTOVOLTAIC APPLICATIONSThe first feature that PV Modular fuse holders offers, is the 1000V DC or 1100V DC rated voltages. They have been developedto offer a compact, safety and economic protection solution in photovoltaic installations where due to the increase of the power and technologic evolution, no-load voltages above 800V DC can be achieved. Modular fuse holders for 10x38 & 14x51 gPV fuses according IEC/EN 60269 standard. Compact design, with reduced distances. Manufactured with a high quality materials: Silver plated copper contacts and plastic materials with high temperature resistance and selfextinguishable. All the materials are according to the European Directive RoHS (Restriction of the use of certain hazardous substances in electrical material). UL certification (File E359201).POLES MODULES REFERENCE DESCRIPTION I nUPACKING(A)(V DC)Uni./BOX48515048515148515248515300COMPATIBLEgPV FUSE-LINKS FOR PHOTOVOLTAIC APPLICATIONSPAGE02STANDARDS IEC 60269-1IEC 60269-2EN 60269-1EN 60269-2UL4248-18APPROVALSCOMPATIBLE CONNECTION ACCESSORIES:PHASE BUSBARS AND ACCESSORIESSEECYLINDRICALWITHINDICA TORWITHINDICA TOR65x20PML1500VDCFUSE HOLDERS FOR PHOTOVOLTAIC APPLICATIONS 1500 VDC▶ Fuse holder for cylindrical fuse link size 10x85 gVP and 10/14x85 gPV.▶ Modern compact design.▶ Ventilation zones optimized for a better heat dissipation.▶ Manufactured with a high quality materials.· Silver plated copper contacts.· Plastic materials with high temperature resistance and self-extinguishable.· All the materials are according to the European Directive RoHS (Restriction of the use of certain hazardous substances in electrical material) and also halogen free.POLES MODULE REFERENCE DESCRIPTION I n U PACKING (mm)(A)(V DC)Uni./BOXFUSE HOLDERS PHOTOVOLTAICSTANDARDSIEC/EN 60269-1IEC/EN 60269-2UL4248-1UL4248-19UL486ECOMPATIBLEgPV FUSE-LINKS FORPHOTOVOLTAICAPPLICATIONSPAGE02COMPATIBLECONNECTIONACCESSORIES:PHASE BUSBARS ANDACCESSORIESSEECYLINDRICAL|7|FUSE HOLDERSPHOTOVOLTAICFUSE HOLDERS FOR PHOTOVOLTAIC APPLICATIONS 1500 VDCFUSE-LINK SIZE SELECTORTECHNICALPMLPOSITION 10x85POSITION 10/14x85Moving part allows the use of 10x85 and 10/14x85 fuselinks Handle tie System lock that avoids the unauthorized use of the fuseholder.Sealed by wire Free space for labeling For flexible wire section ≤6mm For solid wire section ≤10mm Manual assembly Assembly pin8482002482001 FUSE BASES PHOTOVOLTAICClip contact for φ10 cylindrical fuse links. Screw fixation and PCB versions. Manufactured in tinned bronze. All the materials are accordingto the European Directive RoHS (Restriction of the use of certain hazardous substances in electrical material).CLIP CONTACT FOR φ10 FUSE-LINKSREFERENCE DESCRIPTION I n P d max PACKING(A)(W)Uni./BOXφ3,5φ3,5SIZE L(mm)10x383210x8579,60,710102φ2,5LSIZE L(mm)10x384210x8589,6φ10 CLIP CONTACT FOR PCB|9|ST1000V DCNH1250A 354175FUSE BASESPHOTOVOLTAICNH FUSE BASES FOR PHOTOVOLTAIC APPLICATIONSFuse bases for NH fuse-links. Available in sizes NH1(250 A), NH2(400 A) and NH3(500 A). Manufactured with a high quality materials. Silver plated copper contacts. Plastic materials with high temperature resistance and self-extinguishable. All the materials are according to the European Directive RoHS. For DIN rail mounting or with screw fixing. Single-phase type. Connec-tion by screws. Contacts with double spring in order to obtain an optimum operation. Wide range of accessories that enables IP20 protection index: contact covers, fuse link covers and partition walls. Multi-pole units can be made with connection acces-sories. Manufactured according IEC, VDE and DIN standards. UL certification (File E359201).DESCRIPTIONREFERENCEUPACKING(V DC)SINGLE POLEDIN RAIL-SCREW FIXING / SCREW CONNECTION 35417210001NH2400A DIN RAIL-SCREW FIXING / SCREW CONNECTION 35417510001NH3500ADIN RAIL-SCREW FIXING / SCREW CONNECTION 35418010001STANDARDSIEC 60269-1IEC 60269-2EN 60269-1EN 60269-2UL4248STANDARDSVDE 0636DIN 43620COMPATIBLE gPV NH1 & NH3 FUSE-LINKS FOR PHOTOVOLTAIC APPLICATIONSPAGE04COMPATIBLETERMINAL COVERS, FUSE-LINKS COVERS AND IP20 PROTECTION KITSSEE NHNH1NH2NH3357010NH FUSE BASES ACCESSORIESMICROSWITCHES FOR NH LINKSREFERENCE DESCRIPTION PACKINGUni./BOX357010MICROSWITCH FOR NH1 & NH3 FUSE LINKS1/12REFERENCE POWER DISSIPATION POWER DISSIPATION PREARCING I2t(W @ 0,7 In)(W @ In)(A2s)1000V600VDC 1000VDC600VDC1000VDC600VDC1000VDC600VDC4916014919010,310,310,760,760,350,35Prospective current (A rms)*1100V DC|1011|4922021,283,420,84922051,162,91134922101,102,6565P r e a r c i n g t i m e (s )Prospective current (A rms)REFERENCE POWER DISSIPATIONPOWER DISSIPATIONPREARCING I 2t(W @ 0,7 In)(W @ In)(A 2s)|P r e a r c i n g t i m e (s )Prospective current (A)REFERENCEPOWER DISSIPATIONPOWER DISSIPATIONPREARCING I 2t(W @ 0,7 In)(W @ In)(A 2s)1000V DC1000V DC 1000V DC 1000V DC 3732105,212,5623732156,315,51223732256,716,63023732307,5185623732358,220121014x51NH1NH2NH3t a A1(ºC)400,92450,90500,87550,8513|ELECTRONIC CYLINDRICAL PHOTOVOL T AICRAPIDPLUS NH SPECIAL FUSESDOMESTIC D & DO TRANSFORMERS17|HEAD OFFICE & FACTORYSILICI, 67-6908940 CORNELLA DE LLOBREGAT BARCELONA SPAINTel. +34 93 377 85 85Fax +34 93 377 82 82EXPORT SALESTel. +34 93 475 08 64Fax +34 93 480 07 75***************NATIONAL SALESTel. 93 475 08 64Fax 93 480 07 76******************www.df-sa.esN H 1 g P V 100V D C。

pET-28a(+) 质粒载体说明书

pET-28a(+) 质粒说明书
产品信息：
货号名称产品形式规格储存VT0331-01 pET-28a(+) Plasmid 液体质粒20μl-20℃
使用说明：
淼灵质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品三个月内通知我司，收到质粒后请短暂离心，取1μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

基本信息：
启动子：T7/lac
复制子：pBR322 ori，F1 ori
终止子：T7 terminator
质粒分类：大肠杆菌载体；蛋白表达质粒
质粒大小：5369bp
质粒大小：N-6*His, N-Thrombin, N-T7, C-6×His
原核抗性：卡那霉素Kanamycin
克隆菌株：DH5α等大肠杆菌
培养条件：37℃，有氧，LB
表达宿主：BL21 (DE3) 等含DE3的大肠杆菌
培养条件：37℃，有氧，LB
诱导方式：IPTG或乳糖及其类似物
5' 测序引物：T7：TAA TACGACTCACTA TAGGG
3' 测序引物：T7-Ter：GCTAGTTA TTGCTCAGCGG
备注：可用镍柱来纯化带组氨酸的融合蛋白。

质粒简介：
质粒图谱：。

BY-28A操作指南

BY-28A操作指南
关于BY-28A的问题连绵不断,其实这灯操作起来很简单,具体说明如下(看图):
灯背面可以分成两部分--以ZOOM FLASH那条线为界.
这里有必要先说下闪光灯曝光控制的基本原理:我们知道闪光灯有一个闪光指数(GN),它等于光圈乘以闪光距离, 所以拍远处的物体要用更大光圈,拍近处要用小光圈;具体到某一距离上用多大光圈就得计算一下,这样很麻烦!所以BY-28A就设计了一个计算尺配合两个滑杆来完成这项任务,就是灯背的上半部分
最上方的是感光度滑杆,把它设在当前相机的感光度值,滑杆下方计算尺中的光圈值会跟着左右移动,注意它们之间那两个黄色和红色的方块--这就是告诉你在此感光度下对应的自动档分别用多大光圈(比如当前ISO为100,那么黄色自动档用F5.6,红色自动档用F2.8),感光度越高,使用的光圈就越小,反之越大.
计算尺下面是焦距滑杆,有4个档位,分别对应镜头焦距85mm/50mm/35mm/28mm,注意这里指的是等效焦距! 28A的灯头可以向外拉出以适应不同的焦距,焦距越长则闪光指数越高(厂家一般都以50mm焦距来标明闪光指数),但闪光覆盖视角会减小, 所以应该让灯头焦距设在不高于镜头焦距的那一档上,否则拍摄的照片会有暗角(比如镜头焦距为70mm,那灯头就要设在50mm而不能设在85mm档位), 计算尺中的距离值会随着焦距滑杆的档位变化而左右移动,这就告诉你在当前焦距下不同拍摄距离对应的光圈应设在多大.
除了上半部分,下面就一开关和两LED灯,很简单--开关上四个档位从左至右依次是"关闭/手动(全功率输出)/黄色自动档/红色自动档".左边的READY灯显示电容充电是否完成,右边的AUTO OK灯用于测试自动档是否起了作用
化,如图。

类弹性蛋白多肽-红色荧光蛋白融合蛋白的表达纯化及细胞相容性

第22卷第1期北华大学学报(自然科学版)Vol.22No.12021年1月JOURNAL OF BEIHUA UNIVERSITY(Natural Science)Jan.2021文章编号:1009-4822(2021)01-0042-05DOI :10.11713/j.issn.1009-4822.2021.01.008类弹性蛋白多肽-红色荧光蛋白融合蛋白的表达纯化及细胞相容性刘㊀宁,崔梅英,王明月,杨泽斌,王㊀浩,毛㊀禹,方楷漪,夏㊀薇,关新刚(北华大学医学技术学院,吉林吉林㊀132013)摘要:目的㊀构建携带红色荧光蛋白(mCherry)标签的类弹性蛋白(ELP)原核表达载体,并表达纯化ELP-mCherry 融合蛋白,探讨ELP-mCherry 融合蛋白与细胞的相容性.方法㊀对利用限制性内切酶Xba I 和Xho I 对mCherry 质粒和pET28a-ELP 载体同时进行双酶切,将酶切产物回收㊁连接并转化DH5α大肠杆菌感受态细胞,构建重组质粒pET28a-ELP-mCherry;将重组质粒转化到BL21(DE3)大肠杆菌感受态细胞中,诱导表达ELP-mCherry 融合蛋白;利用可逆转变循环(ITC)法对ELP-mCherry 融合蛋白进行纯化;通过MTT 法探讨ELP-mCherry 融合蛋白与人胚肾上皮细胞HEK293T 和小鼠胚胎成纤维细胞NIH3T3的细胞相容性.结果㊀DNA 测序结果显示成功构建了ELP-mCherry 原核表达载体;利用BL21(DE3)大肠杆菌表达系统成功表达ELP-mCherry 融合蛋白,通过ITC 法纯化得到了高纯度融合蛋白.MTT 结果表明:在所有ELP-mCherry 蛋白测试浓度下,HEK293T 和NIH3T3细胞存活率接近或超过100%.结论㊀成功构建ELP-mCherry 原核表达载体,成功表达并纯化得到高纯度的ELP-mCherry 融合蛋白,ELP-mCherry 融合蛋白在HEK293T 和NIH3T3细胞中具有良好的细胞相容性.关键词:类弹性蛋白多肽;红色荧光蛋白;原核表达;蛋白纯化;细胞相容性中图分类号:Q943.2文献标志码:A收稿日期:2020-03-08基金项目:吉林省科技发展计划项目(20180101213JC);吉林省人才开发资金项目(201858);吉林省教育厅科学技术研究项目(JJKH20200033KJ);吉林省卫生计生青年科技骨干培养计划项目(2017Q040);吉林市科技创新发展计划项目(201831729);北华大学青年科研创新团队项目.作者简介:刘㊀宁(1996 ),女,硕士研究生,主要从事检验诊断新技术研究,E-mail:842832918@;通信作者:夏㊀薇(1964 ),女,博士,教授,硕士生导师,主要从事检验诊断新技术研究,E-mail:xiawei4016@.Prokaryotic Expression ,Purification and Cell Compatibility ofELP-mCherry Fusion ProteinLIU Ning,CUI Meiying,WANG Mingyue,YANG Zebin,WANG Hao,MAO Yu,FANG Kaiyi,XIA Wei,GUAN Xingang(School of Medical Technology ,Beihua University ,Jilin 132013,China )Abstract :Objective To construct elastin-like protein (ELP)prokaryotic expression vector with red fluorescent protein (mCherry)tag,to purify the ELP-mCherry fusion protein and investigate the biocompatibility of ELP-mCherry protein on cells.Method mCherry plasmids and pET28a-ELP plasmids were double digested by restriction endonucleases Xba I and Xho I,which were used to construct pET28a-ELP-mCherry plasmid.The recombinant plasmids were transformed into BL21(DE3)E.coli competent cells to express ELP-mCherry fusion protein.ELP-mCherry fusion was purified by reversible transformation cycle method (ITC).The biocompatibility of ELP-mCherry protein was evaluated on human renal epithelial cells and mouse embryonic fibroblasts by MTT method.Results DNA sequencing result indicated the successful construction of ELP-mCherry plasmid,and ELP-mCherry fusion protein was acquired and purified by using BL21(DE3)E.coli system via ITC method.MTT results showed that HEK293T and NIH3T3treated with ELP-mCherry fusion protein under all tested concentration has a cell viability of100%or more.Conclusion We successfully constructed ELP-mCherry prokaryotic expression vector,and got the high purity ELP-mCherry fusion protein,ELP-mCherry protein had good cell compatibility on HEK293T and NIH3T3.Key words:elastin-like polypeptide;mCherry;prokaryotic expression;protein purification;cell compatibility㊀㊀类弹性蛋白样多肽(elastin-like polypeptide, ELP)是一种由基因工程设计合成的非免疫原性且无热原性的具有良好生物相容性的蛋白质聚合物[1-2],ELP主要是由五肽重复序列单元构成,即Val-Pro-Gly-Xaa-Gly(VPGXG),其中Xaa是除脯氨酸以外的任一种氨基酸[3-5].ELP具有可逆相变循环(Inverse transitioncycling,ITC)的特性,即温度低于其相变温度,ELP多肽以高度可溶的形式存在于水溶液中;若温度高于其相变温度,ELP开始聚集,形成不溶物聚集体,且该过程可逆[6].由于ELP具有的这种特殊性质,在大肠杆菌中高产量表达的ELP蛋白可以利用可逆相变的特性进行快速纯化[7].红色荧光蛋白(mCherry)是SHANER 等[8]将发色基团mRFP进行位点突变得到的一种单体红色荧光蛋白[9],mCherry的荧光强度高且稳定性好[10].KALIMUTHU等[11]将含有mCherry基因的慢病毒颗粒转染人间充质干细胞,表达红色荧光mCherry基因,用生物发光成像法追踪间充质干细胞在荷瘤小鼠中的迁移.在本研究中,基于ELP蛋白的独特优势及mCherry的荧光成像特性,我们构建了ELP-mCherry原核表达载体,表达纯化了ELP-mCherry 融合蛋白,探讨了其与人肾上皮细胞HEK293T和小鼠胚胎成纤维细胞NIH3T3细胞的相容性,为设计开发新型的荧光示踪组织工程材料奠定了基础. 1㊀材料与方法1.1㊀细胞、主要仪器和试剂pET28a-ELP和pEGFP-N1-mCherry质粒(Origene 公司,美国);限制性内切酶Xba I与Xho I(生工生物工程(上海)有限公司);大肠杆菌BL21菌株为本实验室保存菌株;HEK293T细胞㊁NIH3T3细胞复苏自本实验室冻存细胞;胰蛋白胨㊁酵母提取物㊁卡那霉素㊁异丙基-β-D-硫代半乳糖苷(IPTG)(生工生物工程(上海)有限公司);多功能酶标仪Tecan Spark(上海帝肯公司);PCR仪㊁核酸电泳以及SDS-PAGE电泳所使用的电泳仪与电泳槽(Bio-Rad公司,美国).1.2㊀ELP-mCherry原核表达载体的构建及鉴定根据GenBank中mCherry基因的核苷酸序列设计包含mCherry基因编码框的引物(生工生物工程(上海)有限公司),并在引物两端分别添加Xba I和Xho I酶切位点.以pEGFP-N1-mCherry为模板通过PCR扩增获得mCherry片段,凝胶回收mCherry片段.mCherry片段和pET28a-ELP质粒分别用1μL限制性内切酶Xba I和Xho I酶切,回收目的片段,利用T4DNA连接酶在4ħ连接过夜,将连接产物转化到DH5α感受态细胞,接种于含有卡那霉素的LB平板上过夜培养.从平板培养基中挑取单个菌落,170r/min摇床过夜,提取质粒后用Xba I限制性内切酶进行单酶切验证,再用Xba I和Xho I进行双酶切及1%琼脂糖凝胶电泳检测(Bio-rad),并将构建的重组质粒送至生工生物工程(上海)股份有限公司进行测序分析.1.3㊀ELP-mCherry融合蛋白的原核表达将构建的pET28a-ELP-mCherry质粒转化到大肠杆菌感受态细胞(BL21)中,过夜培养后挑取单克隆菌落,接种于20mL含有卡那霉素的LB培养基中,37ħ㊁170r/min摇菌过夜;第2天按1ʒ50接种于1L含有卡那霉素的LB培养基中,当OD600达到0.6时,加入IPTG(终浓度1mmol/L),37ħ条件下培养6h诱导蛋白表达;离心收集菌体,PBS重悬菌体沉淀后加入苯甲基磺酰氟(PMSF,终浓度1mol/L),应用超声破碎菌体,离心收集上清和沉淀,通过SDS-PAGE电泳分析蛋白的表达情况. 1.4㊀ELP-mCherry融合蛋白的纯化利用ITC法快速纯化ELP-mCherry融合蛋白,将裂解液上清用NaOH调节pH至9.0,4ħ振荡过夜;第2天将裂解液上清在4ħ下,5000r/min离心90min,去除不溶性蛋白沉淀;将分离的上清恢复至室温后,置于37ħ摇床,250r/min震荡3h,在3h 内分3次加入NaCl至终浓度为1mol/L,37ħ离心收集沉淀,PBS重悬沉淀.以上过程重复两个循环,通过SDS-PAGE电泳分析蛋白的纯化情况.1.5㊀ELP-mCherry融合蛋白的细胞相容性用MTT法检测ELP-mCherry融合蛋白对HEK293T细胞和NIH3T3细胞增殖能力的影响.将对数生长期的HEK293T细胞和NIH3T3细胞以5000个/孔接种于96孔板中,置入37ħ㊁5%CO2培养箱中培养24h;第2天每孔中加入终浓度25㊁34第1期刘㊀宁,等:类弹性蛋白多肽-红色荧光蛋白融合蛋白的表达纯化及细胞相容性50㊁100㊁200㊁300μg /mL 的ELP-mCherry 融合蛋白,每个ELP-mCherry 融合蛋白浓度做5个平行孔.培养48h 后每孔加入20μL 的MTT,37ħ孵育4h 后,每孔再加入150μL 二甲基亚砜(DMSO),沉淀充分溶解后在酶标仪(TECAN M200)上测定OD 490时每孔的吸光度值.2㊀结㊀㊀果2.1㊀ELP-mCherry 原核表达载体的构建将从mCherry 质粒中获得的PCR 产物与pET28a-ELP 质粒分别用限制性内切酶Xba I 和Xho I 双酶切后,酶切产物经0.8%琼脂糖凝胶分离,将目的片段凝胶回收后连接,连接产物转化到Kana 平板,获得阳性克隆.提取质粒通过酶切进行验证,双酶切产生的片段与预期大小一致,提示pET28a-ELP-mCherry 重组质粒初步构建成功.见图1.DNA 测序结果显示mCherry 基因被成功插入表达载体中,没有发生碱基突变和移位,表明pET28a-ELP-mCherry 重组质粒构建成功.见图2.M.DL 5000DNA marker;1.Xba I 单酶切结果;2.Xba I 和Xho I 双酶切结果.图1pET28a-ELP-mCherry 重组质粒的酶切鉴定Fig.1Identification of pET28a-ELP-mCherry㊀㊀recombinant plasmid by enzymedigestion图2pET28a-ELP-mCherry 重组质粒的测序结果Fig.2Sequencing result of pET28a-ELP-mCherry recombinant plasmid2.2㊀ELP-mCherry 融合蛋白的原核表达将pET28a-ELP-mCherry 表达载体转入大肠杆菌感受态细胞(BL21)中,诱导ELP-mCherry 重组蛋白表达,SDS-PAGE 电泳结果显示:在细胞裂解液的上清在分子量30kDa 左右出现明显的蛋白条带,与ELP-mCherry 融合蛋白的预计分子量相符,说明ELP-mCherry 融合蛋白存在于细胞裂解液上清中,然后再利用ITC 法进行快速纯化.见图3.M.蛋白分子量标准;1.菌体细胞裂解液上清;2.菌体细胞裂解液沉淀.图3ELP-mCherry 重组蛋白的原核表达Fig.3Prokaryotic expression of ELP-mCherryrecombinant protein2.3㊀ELP-mCherry 融合蛋白的纯化利用ITC 法纯化ELP-mCherry 融合蛋白,裂解液上清通过两轮ITC 循环的结果显示:电泳条带纯化后的ELP-mCherry 融合蛋白在30kDa 位置显示单一条带,未出现明显的蛋白杂带,且蛋白分子量与ELP-mCherry 融合蛋白相符.因此,可以确定纯化蛋白为ELP-mCherry 蛋白.见图4.M.蛋白分子量标准;1.纯化后的ELP-mCherry 融合蛋白.图4ELP-mCherry 融合蛋白纯化Fig.4Purification of ELP-mCherry fusion protein2.4㊀ELP-mCherry 融合蛋白细胞相容性分析将不同浓度ELP-mCherry 融合蛋白(25㊁50㊁100㊁200㊁300μg /mL)分别处理HEK293T 细胞及NIH3T3细胞48h 后,通过MTT 法检测细胞的存活率.处理48h 后,HEK293T 细胞的存活率在各个浓44北华大学学报(自然科学版)第22卷度下都超过100%,NIH3T3细胞在测试所有浓度下也接近100%,这些结果说明ELP-mCherry 融合蛋白在两种细胞中都具有良好的细胞相容性.见图5.图5ELP-mCherry 融合蛋白的细胞相容性Fig.5Cell compatibility of ELP-mCherry fusion protein3㊀结㊀㊀论利用传统亲和层析法(麦芽糖结合蛋白MBP㊁谷胱甘肽S-转移酶GST㊁多聚组氨酸标签His㊁多聚精氨酸Arg)可以快速获得高纯度的目的蛋白,然而纯化介质成本较高,因此,迫切需要开发一种成本低廉的高纯度蛋白量产方法[12-13].1999年,MEYER 等[14]首次将ELP 与其他蛋白融合表达时发现了其可逆温度相变转换的特性,从此开创了一种新的蛋白纯化方法,称为 ELP 化 ,即将目的蛋白基因与ELP 基因的N 末端或C 末端融合而获得融合蛋白的方法.由于类弹性蛋白多肽类衍生蛋白具有可逆温度相变(低温溶解,高温聚集)特性,应用ITC 法对ELPs 蛋白进行纯化只需要加盐㊁升温(蛋白聚集)㊁离心(收集蛋白沉淀)㊁蛋白复溶几个步骤即可完成,多数情况下只需要2~3轮ITC 纯化即可获得高纯度的目的蛋白,无需昂贵的亲和层析介质,极大地降低了蛋白的生产成本,有利于进行大规模的蛋白质生产.近年来 ELP 化法在重组蛋白纯化㊁改善目的蛋白半衰期㊁提升蛋白产量㊁作为蛋白的递送载体等方面获得了广泛应用[15-18].例如,MOKTAN 等[19]将细胞穿膜肽和促凋亡肽分别融合在ELP 基因的N 末端和C 末端,通过大肠杆菌表达系统制备了一种新型的蛋白抗肿瘤药物SynB1-ELP-KLAK,结果显示融合蛋白具有显著抑制肿瘤细胞增殖的能力.PHAN 等[20]将两种禽流感H 5N 1抗原与ELP 融合后在转基因烟草中进行融合蛋白表达,并利用ITC 方法成功获得了融合蛋白,结果显示ELP 化方法在烟草表达系统中能够显著提高目的蛋白产量.FLOSS 等[21]将anti-HIV-1单克隆抗体2F5与ELP 融合后在中国仓鼠卵巢细胞(CHO)也获得了成功表达,结果显示单克隆抗体与ELP 融合并未影响2F5与抗原的结合活性,显示ELP 化法在哺乳动物表达系统中的有效性.本研究结果显示:通过构建ELP-mCherry 融合蛋白的表达载体,并将其转入大肠杆菌中进行融合蛋白表达,应用ITC 法对融合蛋白进行纯化,得到纯度较高的ELP-mCherry 融合蛋白.鉴于ELP 蛋白在细胞中极佳的细胞相容性,我们随后检测了ELP-mCherry 融合蛋白在HEK293T 细胞和NIH3T3细胞中的细胞相容性,结果显示融合蛋白在两种细胞的存活率超过或接近100%,提示引入mCherry 并未降低ELP 的细胞相容性,为接下来的组织工程应用奠定了良好的材料基础.mCherry 红色荧光蛋白的最大激发波长为587nm [22-24],具有结构稳定㊁表达量高㊁测定简便等优点,因此,被广泛用来对组织细胞定位进行示踪.将ELP 与mCherry 制备成ELP-mCherry 融合蛋白,既可以利用ELP 的可逆温度相变转换特性纯化的融合蛋白,又可以利用mCherry 高度稳定的红色荧光追踪其分布[25],因此,可作为理想的集示踪与支架功能于一身的生物材料用于皮肤损伤愈合㊁组织修复等工程应用.综上所述,本研究成功构建pET28a-ELP-mCherry 表达载体,表达并纯化ELP-mCherry 融合蛋白,ELP-mCherry 融合蛋白对HEK293T 细胞和NIH3T3细胞具有良好的细胞相容性,此研究为开发新型的组织工程示踪材料奠定了基础.参考文献:[1]MECO E,LAMPE K J.Impact of elastin-like proteintemperature transition on PEG-ELP hybrid hydrogel pro-perties[J].Biomacromolecules,2019,20(5):1914-1925.[2]MULLERPATAN A,CHANDRA D,KANE E,et al.Purification of proteins using peptide-ELP based affinityprecipitation[J].Biotechnol,2020,309:9-67.[3]GONZALEZ V J,GIROTTI A,MUNOZ R,et al.Self-54第1期刘㊀宁,等:类弹性蛋白多肽-红色荧光蛋白融合蛋白的表达纯化及细胞相容性assembling ELR-based nanoparticles as smart drug-delivery systems modulating cellular growth via Akt[J].Bioma-cromolecules,2019,20(5):1996-2007.[4]TA D T,VANELLA R,NASH M A.Bioorthogonal elastin-like polypeptide scaffolds for immunoassay 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[23]金权鑫,许嘉珍,魏枫,等.MuSK-mCherry融合荧光蛋白的构建及对重症肌无力患者MuSK抗体的检测[J].中国免疫学杂志,2014,30(10):1369-1373. [24]赵俊丽,王东阳,南天惠,等.红色荧光蛋白遗传标记腺病毒衣壳蛋白pⅨ的实验研究[J].陕西师范大学学报(自然科学版),2011,39(6):60-63. [25]樊晋宇,崔宗强,张先恩.红色荧光蛋白的光谱多样性及体外分子进化[J].生物化学与生物物理进展,2008,35(10):1112-1120.ʌ责任编辑:陈丽华ɔ64北华大学学报(自然科学版)第22卷。

海康威视监控主机说明书

监控系统使用说明书惠州市中鑫电子工程技术有限公司目录1. 系统简介 (3)1.1. 统功能特点 (3)1.2. 运行环境 (4)1.3. 软件介绍 (4)2. 程序安装 (5)2.1. 硬件安装 (5)2.2. 软件安装 (5)3. 操作指南 (5)3.1. S ERVER（服务器） (5)3.1.1. 登录 (5)3.1.2. 指示灯 (6)3.1.3. 云台及摄像头的控制 (6)3.1.4. 电子地图 (6)3.1.5. 常用功能键 (7)3.1.6. AV Sentry系统设置 (7)3.1.6.1. 进入A V Sentry系统设置 (7)3.1.6.2. 选择通道 (7)3.1.6.3. 系统功能选项卡组 (8)3.1.7. 事件查看 (12)3.1.8. 用户管理 (13)3.1.9. 电子地图 (15)3.1.10. 系统辅助操作 (16)3.1.10.1. 系统辅助操作 (16)3.1.10.2. 实时抓图 (16)3.1.10.3. 抓图浏览 (16)3.1.10.4. 云台预置点操作 (16)3.1.10.5. 文字聊天室 (17)3.1.10.6. 音量调节 (17)3.1.10.7. 视频通道切换 (17)3.1.10.8. 矩阵切换控制 (17)3.1.10.9. 刷新网络远程报警 (17)3.1.10.10. 外部接口 (17)3.1.10.11. 视频参数调节 (18)3.1.10.12. 远程连接控制 (18)3.1.11. 屏幕显示控制 (18)3.1.12. 主界面的其余显示项 (18)3.1.13. 退出系统 (18)3.2. 视频回放 (19)3.2.1. 登录 (19)3.2.2. 回放屏幕显示控制 (19)3.2.3. 选择回放文件 (19)3.2.4. 同步播放 (19)3.2.5. 备份 (19)3.2.6. 静音设置 (20)3.2.7. 图像快照 (20)3.2.8. 文件选择 (20)3.2.9. 图像浏览－打印 (20)3.2.10. 播放功能键 (20)3.2.11. 视频回放控制栏 (20)3.2.12. 远程回放 (21)3.3. C LIENT（网络浏览器） (21)3.3.1. 登陆 (21)3.3.2. 显示通道及指示灯 (21)3.3.3. 常用功能键 (21)3.3.4. 云台控制 (22)3.3.5. 视频回放 (22)3.3.6. 系统设置 (22)3.3.6.1. 进入系统设置 (22)3.3.6.2. 系统设置选项卡组 (22)3.3.6.3. 用户管理 (24)3.3.7. 屏幕显示 (24)3.3.8. 系统辅助操作 (24)3.4. 如何注册 (25)4. 许可协议 (25)1.系统简介AV Sentry网络音视频数码录像系统是当今多媒体、图像处理、计算机等各项最新技术高度结合的产品。

pET28a

BssH II(1534) EcoR V(1573) Hpa I(1629)
PshA I(1968) Bgl I(2187) Fsp I(2205) Psp5 II(2230)
T7 promoter primer #69348-3
pET upstream primer #69214-3 Bgl II
2995
Enzyme # Sites Locations
BstEII 1
1304
BstXI 3
925 1054 1177
BstYI 9
132 198 401 687 1899
2416 3865 3876 4675
Cac8I 40
CjeI
26
CjePI 30
ClaI
1
4117
CviJI 86
CviRI 22
MetGlyArgGlySerGluPheGluLeuArgArgGlnAlaCysGlyArgThrArgAlaProProProProProLeuArgSerGlyCysEnd
...GGTCGGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCC pET-28b(+) ...GlyArgAspProAsnSerSerSerValAspLysLeuAlaAlaAlaLeuGluHisHisHisHisHisHisEnd
T7•Tag
TATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAA

pET-28a(+)质粒载体说明书-pet-28a.

pET-28a(+)质粒载体说明书-pet-28a.
1、增加收菌次数,相对提高了质粒的量5261,这样的话裂解液的量可以适当增加。

2、裂解要充分,变性和复性按说明应该是不超过5分钟,合理控制时间,一般在3分到4分半的时4102间都是可以的。

3、过柱子时,吸附的时间尽量长一些,可以在这期间做做其他实验或者吃个饭什么的。

4、如果没有必要,不使用去蛋白液,因为每过一遍柱子,其损耗越大1653,不过这得根据说明的菌种而定；
4、最后洗脱回收的时候,要单方面提高浓度,就要适当减少洗脱液的量,我一般都是回收到40~50μL的,同时要增加洗专脱次数,一般两三次足矣。

pET28a

3397
BsiEI 5
169 1908 3140 3564 4426
BslI
23
BsmI 2
4310 4387
BsmAI 6
820 1225 1351 1738 2865
4442
BsmBI 3
1738 2865 4442
BsmFI 4
584 2125 2495 5342
BsoFI 48
Bsp24I 12
370-386 369 270-287 207-239
158-203 140-157 26-72 773-1852 3286 3995-4807 4903-5358
The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.
T7 promoter
lac operator
Xba I
rbs
AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGA
Nco I
His•Tag
Nde I Nhe I

Minca 28摄影机用户手册说明书

HOW TO USE YOUR minER 28
t There are 4 steps to good pictures ,
with your Minca 28
11-. LOAD.
2. ADJUST SHUTTER. ,
3. ADJUST LENS. 4. TAKE THE PICTURE.
About Your Minca 28
Unroll about 4 inches of paper leader and insert the end in the slot of the empty take-up spool.
Replace the back, making sure the lock is secure to avoid accidental fogging of the film .
There are two markings on the lens mount: "I," meaning instantaneous, for snapshot pictures; "T," meaning time, for time exposures (use this only for inactive subjects, indoors, at night, or in very duJ/light).
Place the empty spool opposit!} the film-winding knob, taking care that it 'is all the way in.
Place the roll of film in the other compartment, with the paper leader in the position shown above.

以pET-28a表达载体

2 含T7噬菌体启动子的表达载体（例如pET系列载体）外源基因表达受T7噬菌体RNA聚合酶调控，具有氨苄或者卡那抗性，多克隆位点置于T7噬菌体RNA聚合酶启动子之后 3 带有&噬菌体PL 启动子的表达载体（ pPLc系列 pKC30 等）受温度敏感性阻抑物cIts857调控，低温时能抑制PL 驱动的转录，高温不能 4 用碱性磷酸酶启动子（phoA）和信号序列
生科0801 黄开松
克隆基因的表达
目前有5套发展成熟的表达系统
1在大肠杆菌中表达 2在哺乳动物细胞中表达 3在酿酒酵母中表达 4在枯草芽孢杆菌中表达 5在培养的昆虫细胞中表达
在大肠杆菌中表达克隆化基因
• 选择合适启动子和载体系统
1带IPTG诱导启动子的表达载体例如tac trc lac 启动子 (pUC,pTZ,pSK,pGEM等）
• 目标蛋白的体外纯化（1）灌制好Ni2+-NTA亲和层
析柱，用去离子水进行缓慢洗脱，避免在柱床中引入气泡。（2）用10倍柱床体积的starting buffer进行预平衡，上样，将细胞破碎后的上清液注入Ni2+-NTA亲和层析柱中。（3）用10倍ml的starting buffer进行漂洗，收集滤过液。（4）开始用5倍柱床体积的含20 mM咪唑的starting buffer进行洗脱，并对洗脱液进行收集。（5）依次用5倍柱床体积的含40 mM，60 mM，80 mM， 100 mM，200 mM，300 mM和500 mM咪唑的starting buffer进行洗脱，分别对洗脱液进行收集。（6）通过SDS-PAGE检测回收的效果，确定咪唑最合适洗脱浓度。（7）从每管收集的滤出液取出10μl的样品，加入10μl的2X SDS凝胶加样缓冲液，摇匀。（8）沸水浴处理3min。（9）取出样品，将10μl样品全部上样于适当浓度的SDS聚丙烯酰胺凝胶上电泳。

pet28a原核表达载体的表达原理

pet28a原核表达载体的表达原理Pet28a原核表达载体是一种常用于原核生物中蛋白质表达的载体。

它的表达原理基于其构建的特点和作用机制。

Pet28a原核表达载体的构建特点是将多个功能元件组合在一起，以实现高效的蛋白质表达。

主要包括启动子、编码序列、标签序列和终止子等。

Pet28a的启动子是一段能够识别并与细菌RNA聚合酶结合的序列，用来启动基因的转录。

细菌RNA聚合酶在启动子的识别下，能够将DNA的信息转录成mRNA，作为蛋白质合成的模板。

编码序列是Pet28a中最重要的部分，它包含了目标蛋白质的编码信息。

编码序列是由一串三个核苷酸组成的密码子序列，每个密码子对应一个氨基酸。

在细胞内，mRNA会被核糖体识别并通过翻译作用将其转化为氨基酸序列，从而合成出目标蛋白质。

Pet28a载体中还包含了标签序列，用于方便对目标蛋白质进行检测和纯化。

常用的标签序列有His标签和GST标签。

His标签是一段连续的组氨酸序列，能够与金属离子亲和层析柱结合，实现目标蛋白质的纯化。

GST标签是谷胱甘肽S-转移酶标签，能够与谷胱甘肽结合，实现目标蛋白质的纯化。

Pet28a载体的终止子是一段能够识别并终止转录的序列。

在RNA聚合酶到达终止子时，转录过程会终止，mRNA链被释放出来，进一步被核糖体翻译为蛋白质。

总结起来，Pet28a原核表达载体的表达原理是通过启动子识别和RNA聚合酶的转录作用，将编码序列转录为mRNA。

然后，mRNA通过核糖体的翻译作用，合成目标蛋白质。

最后，通过标签序列的存在，可以对目标蛋白质进行检测和纯化。

Pet28a原核表达载体的表达原理使其成为研究人员在原核生物中高效表达蛋白质的重要工具。

它的构建特点和作用机制为研究人员提供了方便、快捷和可靠的蛋白质表达平台。

通过对Pet28a载体的合理设计和选择，可以实现目标蛋白质的高效表达，为生物学研究和工业生产提供了有力支持。

pentacam中文手册

PENETACAM 操作手册（中文）
有 LED 的开关，“ON” Pentacam HR 的数据传输。（5-poles）
2.7 起步
2.7.1 设置和安装设备
在第一次使用前，Pentacam检查工作站必需由我们的服务部门或授权经销商来进行设置和连接。
请把CD-ROMs妥善保存，它们包括 Pentacam的软件和标准数据。
PENETACAM 操作手册（中文）
2.2 主机
OCULUS的 Pentacam是一旋转的
检查过程中的Scheimpflug图像以及所有
Scheimpflug相机。旋转测量过程拍摄了3维的的图像均传输至计算机。当检查结束后，计算
Scheimpflug 图像，并且通过旋转对中心点阵机计算出3维眼前节模型，再从中得到其他信
2.4 废弃
欧洲议会2003年1月27日的
2002/96/EC规定要求所有的电子电气产品不得通过家庭垃圾抛弃而应予以回收。
产品的包装为可回收材料。本设备的金属部件必需送至金属加工回收。塑料和电子部件以及线路板必需以电子垃圾处理。所有的废弃物的处理必需符合相应国家的规定。具体情况请咨询您所在区域的行政相关部门或者废品处理机构。
如果您放置Pentacam的房间很冷，或者在寒冷环境中运送Pentacam，那么在环境温度由冷转暖时，Pentacam的光学器件会有起雾现象。请在使用Pentacam前给设备足够的时间来适应新的环境。
根据IEC 601 – 1规定的Pentacam运输和储藏条件：
室温：-40°C ~+70°C 湿度（包含）：10% ~ 100% 气压：500 hPa ~ 1060 hPa
OCULUS Optikgeräte 管理及服务团队