bIOMARKERS生物标志物

• Control series (3 jars)
• 6 series run with fungicide (3 jars/series)
• first fungicide series 4 ml fungicide
:128 ml
of water
• diluted 5 times for each successive 5 series
Initial Purpose
• To observe any effects of the fungicide on the red worms
• To determine ld50 for our population of red worms
SOIL ECOTOXICOLOGY EXPERIMENT
• pesticide added after week 1
• Data collected weekly
• worms sorted and analyzed for lesions, swelling, writhing, Number on and below surface
fungicide concentration series
Further objectives
• Additional objectives of this experiment: • 1. examine effects of pollution on non-
target organisms • 2. Accuracy of results, if any, in real
conclusions
• The highest fungicide concentration used (4 times the suggested concentration) appears to have a fairly strong impact on the worms
• The soil sample from the oil container was not a good habitat for the worms since there was a fairly immediate drop in the worm count two weeks after the worms were introduced into this soil
• Materials:
• 24 pint mason jars with lids
• Worms
• Soil mixture - composed of silica sand, kaolin
clay, peat moss, CaCO3, water • Litmus paper
• Pesticide - 0.88%
world situations
• 3. Consider effects of toxin on different organisms
• 4. How this experiment would be improved if carried out for a longer time and replicated
Ld50 & LC50
• Ld50 - median lethal dose (amount of a chemical in one dose that will kill 50% of the population)
• Lc50 - concentration of material in air that will kill 50% of the test subjects
container
mortality
• Mortality is defined in this experiment as lack of movement in response to stimulus or absence, since the worms disintegrate rapidly
results
• Slight trend in mortality rates and increased fungicide concentration
• in soil samples from jason and lindsey, mortality rate low except in soil taken from oil container site
is not supported
• pH of each soil was taken after soil was mixed and again after the fungicide was added
• Initial pH of the soils were about 6.0 6.5
• pH after addition of fungicide ranged from 4.5 (in the more highlyconcentrated soils) to 5.5 (in the less highly-concentrated soils)
Biomarkers
by heather seavolt and stephanie mCPHERSON
Types of biomarkers
• DNA, RNA or protein adducts and enzymes • cytogenic markers • tissue markers and whole body alterations • behavioral markers • measure toxicants in body fluids & tissues • death of marker animals
1-
(4-CHLOROPHENOXY)-3,3-
DIMETHYL-1-
(1h-1,2,4-
TRIAZOL-1-YL)-2-
BUTANONE)
Experiment layout
• 10 worms added to each jar
• Worms allowed to adjust to new soil habitat
• 4th dilution: 1/16 the concentration of series 1
• 5th dilution: 1/32 the concentration of series 1
• Samples of lindsey and jason’s soil • 1. sample from the copper pipe area • 2. sample from site 1 • 3. sample from around suspicious oil
• The other soils with varying concentrations of fungicides provided no worse a habitat for the worms than did the control series
• overall reproductive ability did not appear to be altered
• 1st dilution: 1/2 the fungicide concentration of series 1 (2:128 ml)
• 2nd dilution: 1/4 the concentration of series 1 (recommended concentration)
• 3rd dilution: 1/8 the concentration of series 1
• 5. Ethical issues involved in animal experimentation
data
M a c in to s h P IC T im a g e fo rm a t
is n o t sIC T im age form at