通络救脑注射液及其有效成分对拟缺血损伤人脑微血管内皮细胞的保护作用

通络救脑注射液及其有效成分对拟缺血损伤人脑微血管内皮细
胞的保护作用
李卫红;王东坡;李兴广;林燕
【期刊名称】《安徽中医学院学报》
【年(卷),期】2011(30)6
【摘要】目的通过观察通络救脑注射液及其有效成分对拟缺血损伤人脑微血管内皮细胞活性的影响,以及对血管生成素-1(angiopoietin-1,Ang-1)及其受体内皮特异性酪氨酸激酶受体-2(endothelial-specific tyrosine kinase receptor 2,Tie-2)表达的影响,以评价其对拟缺血损伤人脑微血管内皮细胞的保护作用.方法利用氧糖剥夺方法复制人脑微血管内皮细胞拟缺血损伤模型,通络救脑注射液及其有效成分分别作用不同时间后,采用四甲基偶氮唑盐染色法测定细胞活性,采用乳酸脱氢酶(lactate dehydrogenase,LDH)漏出率检测细胞损伤程度,采用蛋白印迹法检测内皮细胞Ang-1和Tie-2表达水平.结果通络救脑注射液可显著提高拟缺血损伤人脑微血管内皮细胞活性,降低LDH漏出率,增加内皮细胞Ang-1和Tie-2的表达,两个有效成分发挥疗效的时间有所不同,复方注射液作用明显优于两个有效成分.结论通络救脑注射液对拟缺血损伤人脑微血管内皮细胞具有保护作用,其机制与增加Ang-1及其受体Tie-2表达有关,显示中药组方可发挥整合调节的治疗优
势.%Objective To evaluate the protective effects of Tong Luo Jiu Nao (TLJN) Injection, a patent Chinese medicine composed of extracts from Gardenia and Panax Notoginseng, and its active ingredients (including Geniposide and Ginsenoside Rg1) , on ischemia injuried human brain microvascular endothelial cells (hBMECs) , via observing their effects on
the activities of hBMECs and the expression of angiogenin1 (Ang-l) and its receptor Tie-2, an endothelial-specific tyrosine kinase receptor,of mimic ischemia injured hBMECs. Methods The in vitro model of mimic ischemia injuried hBMECs was induced by culturing hBMECs in oxygen-glucose-deprived (OGD) conditions. Then, TLJN Injection and its active ingredients were added into the cultures of OGD-injured cells. At different intervals, methyl thiazolyl tetrazolium staining was employed to assay the activities of hMBECs,the damage level of hMBECs was evaluated using lactate dehydrogenase (LDH) leakage rate,and the expression levels of Ang-1 and Tie-2 were detected by Western blot. Results TLJN Injection could significantly improve the activities of mimic ischemia injured hMBECs,and decrease LDH leakage rate, and up-regulate the expression levels of Ang-1 and Tie-2. Two active ingredients showed different effects at different intervals. The effects of compound TLJN Injection were obviously superior to those of two active ingredients. Conclrision TLJN Injection has protective effects on OGD-injured hMBECs, its mechanisms are related to up-regulating the expression of Ang-1 and Tie-2, showing the advantages of compound Chinese medicine in integrative regulation.
【总页数】5页(P42-46)
【作者】李卫红;王东坡;李兴广;林燕
【作者单位】北京中医药大学基础医学院,北京,100029;北京中医药大学基础医学院,北京,100029;北京中医药大学基础医学院,北京,100029;北京中医药大学基础医学院,北京,100029
【正文语种】中文
【中图分类】R285.5
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