bFGF基因转染MSC目的基因检测

bFGF基因转染MSC目的基因检测
王彦生;于宁
【期刊名称】《中国继续医学教育》
【年(卷),期】2015(7)18
【摘要】Objective To discuss the expression of bFGF gene, after basic fibroblast growth factor (bFGF) exogenous transfection to MSC (bone marrow cells). Methods SD rats were selected. The ectomesenchymal cells were obtained from bone marrow. They were divided into control group, negative control group (transfection exogenous GFP) and experimental group (exogenous bFGF transfection on MSC), in vitro culture, bFGF gene positive expression of MSC were detected by the method of RT-PCR and ELISE in each group. Results RT-PCR showed that bFGF gene expression quantity was (0.73±0.15) in experimental group, it was significantly higher than the control group and the negative control group (P<0.05). ELISA showed that bFGF gene expression was mainly in the cytoplasm,the experimental group bFGF gene expression quantity were [supernatant (5.38±0.45)ng/L, cytoplasm (8.27±0.82)ng/L], it was significantly higher than the other two groups (P<0.05). Conclusion The application of virus mediated gene transfection to transfect exogenous bFGF to MSC can successfully achieve bFGF gene expression.%目的：探讨碱性成纤维细胞生长因子（bFGF）基因外源性转染到MSC（骨髓间充质细胞）后，bFGF基因的表达情况。

方法选取SD大鼠，获取骨髓间充质细胞，分为对照组、阴性对照组（转染
外源性GFP）和实验组（外源性bFGF转染MSC），经过体外培养后，应用RT-PCR和ELISE法检测各组MSC的bFGF基因阳性表达情况。

结果 RT-PCR法显示实验组bFGF基因表达量（0.73±0.15）高于对照组和阴性对照组（P＜0.05）。

ELISA法显示bFGF基因表达主要集中在胞浆，实验组bFGF基因表达量[上清为（5.38±0.45）ng/L，胞浆为（8.27±0.82）ng/L]高于其他两组（P＜0.05）。

结论采用病毒介导基因转染技术能够将外源性bFGF转染至MSC，并能够成功实现bFGF基因表达。

【总页数】2页(P55-56)
【作者】王彦生;于宁
【作者单位】110024沈阳医学院附属中心医院手外科;110024沈阳医学院附属中心医院手外科
【正文语种】中文
【中图分类】R394.3
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