METHOD FOR IMPROVED SEPARATION OF A HYDROPHOBIC OR
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专利名称：METHOD FOR IMPROVED SEPARATION OF
A HYDROPHOBIC ORGANIC SOLUTION
FROM AN AQUEOUS CULTURE MEDIUM 发明人：HENNEMANN, Hans-Georg,SCHAFFER,
Steffen,WESSEL, Mirja,HAAS,
Thomas,CORTHALS, Jasmin,BERSE,
Katharina,HAFKEMEYER, Sabine,PÖTTER,
Markus,ERHARDT, Frank
申请号：EP12806038.1
申请日：20121218
公开号：EP2794898B1
公开日：
20180801
专利内容由知识产权出版社提供
摘要：Improved separation of a hydrophobic organic solution from an aqueous culture medium, comprises preparing an aqueous culture medium comprising a metabolically active cell, contacting the aqueous culture medium with a hydrophobic organic solution, and separating the hydrophobic organic solution from the aqueous culture medium, where a decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell, and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids. Independent claims are included for: (1) use of metabolically active cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, where the enzyme is configured to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active
cell; (2) use of knockout of an enzyme that catalyses one reaction of the beta -oxidation of fatty acids, as part of a genetic make-up of the metabolically active to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active cell; (3) a cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, and a recombinant monooxygenase, preferably alkane hydroxylase; and (4) a reaction mixture, comprising an aqueous solution comprising the metabolically active cell having decreased activity, and a hydrophobic organic solution, where the decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids.
申请人：Evonik Degussa GmbH,EVONIK DEGUSSA GMBH,EVONIK DEGUSSA GMBH 地址：DE
国籍：DE
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