DIP9-Segmentation2
西门子OLM设备使用说明
西门⼦OLM设备使⽤说明西门⼦OLM设备使⽤说明Siemens OLM settings User Guide摘要针对OLM的拨码开关设置以及功能进⾏说明关键词 Profibus,OLM,光纤,DIP拨码开关,环⽹Key Words Profibus,OLM,Optical cable,DIP switch,RingIA&DT Service & Support Page 2-14⽬录1 功能介绍 (4)1.1 485 ⽹段监视 (4)1.2 光路监视 (5)2 拓扑结构 (6)2.1 线型拓扑 (6)2.2 星形结构 (7)2.3 冗余环⽹ (8)3 光纤接收端⼝信号的检测 (11)IA&DT Service & Support Page 3-14PROFIBUS现场总线⼤多使⽤RS485进⾏串⼝的通讯,但有些情况下,也可以将电缆转化为光纤进⾏通讯,OLM就是这样⼀款设备。
图1 OLMOLM使⽤起来⾮常⽅便,不需要进⾏更多的设置,如果没有特殊的情况,⼏乎不需要设置什么,默认状态可以直接使⽤。
但⽤户往往对OLM上的拨码设置开关感到很迷惑,不知怎样设置,这⾥简单做个介绍。
详细内容请参考相关⼿册,这⾥只把⼿册中的关键部分做⼀解释。
1 功能介绍OLM的功能设定都通过模板上的拨码开关进⾏,OLM侧⾯有⼀些说明帮助⽤户进⾏设定。
1.1RS 485 ⽹段监视(Segment monitoring at the RS 485 port)该功能⽤于每个接收设备对于所连接到的RS485⽹段中的故障帧或者⽹络堵塞情况的监测。
当接收设备接收到故障帧或者⽹络堵塞时间长于最⼤的帧发送时间时,或者当⼀秒钟以IA&DT Service & Support Page 4-14上没有接收到信号时,接收到的信号将被“Block”⽽不再被转发到其它端⼝,直到⽹络中能够重新接收到新的报⽂。
QFA2050 MO、QFA2050D MO和QPA2052 MO商业通信模块的基本文档说明书
Room Humidity and Air Quality Sensors:–QFA2050/MO, QFA2050D/MO–QPA2052/MOModbus RTU (RS-485)Basic DocumentationA6V12045847_en--_a Smart Infrastructure 2020-07-23Table of Contents1About this document (3)1.1 Revision history (3)1.2 Before you start (3)2Product overview (5)2.1 Type summary (5)2.2 Product documentation (5)3Wiring (6)4Configuration (7)4.1 DIP switch to configure method selection (7)4.2 Modbus configuration parameters (7)4.3 Modbus registers (8)4.4 DIP switch configuration (9)4.5 Bus termination (11)5Maintenance (12)5.1 Disposal (12)6Appendices (13)6.1 Cyber security disclaimer (13)6.2 FCC (13)2 | 14A6V12045847_en--_aAbout this documentRevision history1A6V12045847_en--_a 3 | 141About this document1.1Revision historyEdition Date Changes Section a2020-07First version.All1.2Before you startThis document may be duplicated and distributed only with the expressed permission of Siemens Switzerland Ltd.These documents were prepared with great care.●The content of all documents is checked at regular intervals.●All necessary corrections are included in subsequent versions.●Documents are automatically amended as a consequence of modifications andcorrections to the products described.Please ensure that you have the latest document revision.If you have any questions or suggestions about this document, or if you have any criticisms or suggestions, please contact your local point of contact (POC) at the nearest branch office. Addresses for Siemens RCs are available at /sbt .MarkupsSpecial markups are indicated in the document as follows:●Numbered lists and instructions with an operation sequence 1.2.Procedures must be performed in the specified order.[➙ X]Reference to a page numberSymbol identificationsWARNINGThis is the symbol for hazard. It warns you of Risks of ply with all measures designated by this symbol to prevent injury or death.NOTICEThis symbol identifies an important notice that you should be aware of when you are using the product.Before using products from Siemens Switzerland Ltd., it is important that you carefully read the documents supplied with or ordered at the same time as the products (equipment, applications, tools, and so on).We assume that users are authorized and trained appropriately and have the technical knowledge required to use our products as intended.Additional information on products and applications is available:Copyright Quality assuranceConventions for text markingDocument use/ request to the readerAbout this document Before you start14 | 14A6V12045847_en--_a●From your Siemens branch office /sbt or at your system suppliers.●From the support team at headquarters ***********************************if no local POC is available. Siemens assumes no liability to the extent allowed under the law for any losses resulting from a failure to comply with the aforementioned points or for the improper compliance of the same.Product overview Type summary2A6V12045847_en--_a 5 | 142Product overviewThe sensors are used in ventilation and air conditioning plants.2.1Type summaryProduct numberSSN No.Measurement parameter Output signal CO 2Temperature Relative humidity QFA2050/MO S55720-S508 --40...70 °C 0…100 % r.h.Modbus RTU QFA2050D/MO S55720-S509 --40…70 °C 0…100 % r.h.Modbus RTU QPA2052/MOS55720-S5100…2000 ppm-40…70 °C0…100 % r.h.Modbus RTU2.2Product documentationProduct numberDatasheet Mounting instructions QFA2050/MO, QFA2050D/MO A6V12046135A6V12031740QPA2052/MOA6V12046144A6V12031740See the datasheets and mounting instructions for detailed information. You can download the above documents at /bt/download .Wiring36 | 14A6V12045847_en--_a3WiringG Operating voltage AC 24 V ±20 % or DC 13.5...35 V G0GND+RS485 Modbus A -RS485 Modbus B RefGND_ISOConfigurationDIP switch to configure method selection4A6V12045847_en--_a 7 | 144Configuration4.1DIP switch to configure method selectionThe sensor is a Modbus RTU (RS-485) slave device and can be configured with a Modbus master when DIP2-Position 7 is OFF (default setting).The sensor has two sets of DIP switches: DIP1 and DIP2 and can be manually configured with DIP switches when DIP2-Position 7 is ON.For details, see DIP switch configuration [➙ 9].4.2Modbus configuration parametersNameRange/numeration Default ConfigurableModbus address 1...2471Baud rate (bps)0 = Auto 1 = 96002 = 192003 = 384004 = 576005 = 768006 = 115200Transmission format (start bits-data bits-parity-stop bits)0 = 1-8-E-11 = 1-8-O-12 = 1-8-N-13 = 1-8-N-20BasicParityEven Odd NoneEven Stop bits 1 / 21Data8 bits (0…255)-Configuration Modbus registers48 | 14A6V12045847_en--_aName Range/numeration Default Identity Slave -Cable length< 600 m-NOTES :●Register 764 (Modbus address) cannot be configured as 246 via a master.Address 246 is reserved for on-event addressing.4.3Modbus registersHolding Register (16-bit)No.DescriptionRangeUnitScaling DefaultR/W1Temperature value -327…327 °C -556.6…620.6 °F°C °F 0.01-R2Temperature reliability 0 – No error1 – Bad reliability, unavailable---R3Relative humidity value 0…100 %%0.01-R 4Humidity reliability0 – No error1 – Bad reliability, unavailable---R5CO 2 value 0…2000 ppm ppm --R 6CO 2 reliability0 – No error1 – Bad reliability, unavailable---R223Temperature offset -100...100 °C -180...180 °F°C °F 0.10RW224Relative humidity offset -100...100 %%0.10RW 225CO2 offset -2000...2000 ppm ppm 1.00RW 401System unit0 – Celsius 1 – Fahrenheitothers - invalid value to be discard--RW1286SW version: Major &Minor versions ----R 1287SW version: Build version----R 764Modbus address 1…247--1RW 765Baud rate0 = Auto 1 = 9600bps 2 = 19200bps 3 = 38400bps 4 = 57600bps 5 = 76800bps 6 = 115200bps--RW766Transmission format (start bits-data bits-parity-stop bits)0 = 1-8-E-11 = 1-8-O-12 = 1-8-N-13 = 1-8-N-2--0RWNOTES:ConfigurationDIP switch configuration4A6V12045847_en--_a 9 | 14●The register number starts at 1.●The sensor rejects a command with an error notice in the event of a multiple-writing command from the master with invalid values. The register values remain unchanged.●Software version format: Major version is 1 byte, minor version is 1 byte and build version is 2 bytes, such as [2.01.33] = 0x02010021.●Supports only the holding register.4.4DIP switch configurationThe Modbus setting can be configured via DIP switches ( DIP2-position 7 must be set to ON).NOTICE●Modbus registers 764…766 are not writable via a master under DIP switches configuration.●When DIP1-position 1...8 are set to 255 and DIP2-position 7 is ON, Modbus parameters are reset to factory default settings: Only Modbus address, baud rate and transmission format are reset to factory default.The sensor has two sets of DIP switches: DIP1 and DIP2.DIP1Address configurationConfigurationDIP switch configuration410 | 14A6V12045847_en--_a2726252423222120Address12345678OFF OFF OFF OFF OFF OFF OFF OFF 0(default)OFF OFF OFF OFF OFF OFF OFF ON 1OFF OFF OFF OFF OFF OFF ON OFF 2OFF OFF OFF OFF OFF OFF ON ON 3OFF OFF OFF OFF OFF ON OFF OFF 4………………………ONONONONONONONON255NOTES:●Modbus address configuration: Valid address range 1…247, others = 0●Newly set values of register 764…766 are not activated for an invalid address;the registers retain the previous value.Baud rateParityStop bit 1)Configuration Function1234567OFF OFF OFF 0 = Auto OFF OFF ON 1 = 9600OFF ON OFF 2 = 19200OFF ON ON 3 = 38400ON OFF OFF 4 = 57600ON OFF ON 5 = 76800ON ON OFF 6 = 115200ONONONothers = AutoOFF OFF 0 = Even (default)OFF ON 1 = Odd ON OFF 2 = No parity ON ONothers = Even OFF 1 (default)ON2OFF Configurable via master ONDIP switchesNOTE:1) Parity + Stop bit only support the following combinations: 1-8-E-1, 1-8-O-1, 1-8-N-1 and 1-8-N-2. Others will be treated as 1-8-E-1.DIP2Baud rate, transmission format, configurationConfigurationBus termination 4A6V12045847_en--_a 11 | 144.5Bus terminationJumper position descriptions:●Jumper position (OFF) = disable terminating resistor (factory setting)●Jumper position (ON) = enable terminating resistorMaintenance Disposal 512 | 14A6V12045847_en--_a5Maintenance5.1DisposalAppendicesCyber security disclaimer 6A6V12045847_en--_a 13 | 146Appendices6.1Cyber security disclaimerSiemens provides a portfolio of products, solutions, systems and services that includes security functions that support the secure operation of plants, systems,machines and networks. In the field of Building Technologies, this includes building automation and control, fire safety, security management as well as physical security systems.In order to protect plants, systems, machines and networks against cyber threats, it is necessary to implement – and continuously maintain – a holistic, state-of-the-art security concept. Siemens’ portfolio only forms one element of such a concept.You are responsible for preventing unauthorized access to your plants, systems,machines and networks which should only be connected to an enterprise network or the internet if and to the extent such a connection is necessary and only when appropriate security measures (e.g. firewalls and/or network segmentation) are in place. Additionally, Siemens’ guidance on appropriate security measures should be taken into account. For additional information, please contact your Siemens sales representative or visit https:///global/en/home/company/topic-areas/future-of-manufacturing/industrial-security.html .Siemens’ portfolio undergoes continuous development to make it more secure.Siemens strongly recommends that updates are applied as soon as they are available and that the latest versions are used. Use of versions that are no longer supported, and failure to apply the latest updates may increase your exposure to cyber threats. Siemens strongly recommends to comply with security advisories on the latest security threats, patches and other related measures, published, among others, under https:///cert/en/cert-security-advisories.htm .6.2FCCThis device complies with part 15 of the FCC rules. Operation is subject to the following two conditions:1.This device may not cause harmful interference;2.This device must accept any interference received, including interference that may cause undesired operation.Changes or modifications not expressly approved by the party responsible for compliance could void the user's authority to operate the equipment.NOTE : This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions,may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:●Reorient or relocate the receiving antenna.●Increase the separation between the equipment and receiver.●Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.●Consult the dealer or an experienced radio/TV technician for help.CAN ICES-3 (B)/NMB-3(B)Issued bySiemens Switzerland LtdSmart InfrastructureGlobal HeadquartersTheilerstrasse 1aCH-6300 Zug+41 58 724 2424/buildingtechnologies© Siemens Switzerland Ltd, 2020Technical specifications and availability subject to change without notice.A6V12045847_en--_a。
常见限制性内切酶识别序列
常见限制性内切酶识别序列(酶切位点)(BamHI、EcoRI、HindIII、NdeI、XhoI等)Time:2009-10-22 PM 15:38Author:bioer Hits: 7681 times在分子克隆实验中,限制性内切酶是必不可少的工具酶。
无论是构建克隆载体还是表达载体,要根据载体选择合适的内切酶(当然,使用T 载就不必考虑了)。
先将引物设计好,然后添加酶切识别序列到引物5' 端。
常用的内切酶比如BamHI、EcoRI、HindIII、NdeI、XhoI等可能你都已经记住了它们的识别序列,不过为了保险起见,还是得查证一下。
下面是一些常用的II型内切酶的识别序列,仅供参考。
先介绍一下什么是II型内切酶吧。
The Type II restriction systems typically contain individual restriction enzymes and modification enzymes encoded by separate genes. The Type II restriction enzymes typically recognize specific DNA sequences and cleave at constant positions at or close to that sequence to produce 5-phosphates and 3-hydroxyls. Usually they require Mg 2+ ions as a cofactor, although some have more exotic requirements. The methyltransferases usually recognize the same sequence although some are more promiscuous. Three types of DNA methyltransferases have been found as part of Type II R-M systems forming either C5-methylcytosine, N4-methylcytosine or N6-methyladenine.酶类型识别序列ApaIType II restrictionenzyme5'GGGCC^C 3'BamHIType II restrictionenzyme5' G^GATCC 3'BglIIType II restrictionenzyme5' A^GATCT 3'EcoRIType II restrictionenzyme5' G^AATTC 3'HindIIIType II restrictionenzyme5' A^AGCTT 3'KpnIType II restrictionenzyme5' GGTAC^C 3'NcoIType II restrictionenzyme5' C^CATGG 3'NdeIType II restrictionenzyme5' CA^TATG 3'NheIType II restrictionenzyme5' G^CTAGC 3'NotIType II restrictionenzyme5' GC^GGCCGC 3'SacIType II restrictionenzyme5' GAGCT^C 3'SalIType II restrictionenzyme5' G^TCGAC 3'SphIType II restrictionenzyme5' GCATG^C 3'XbaIType II restrictionenzyme5' T^CTAGA 3'XhoIType II restrictionenzyme5' C^TCGAG 3'要查找更多内切酶的识别序列,你还可以选择下面几种方法:1. 查你所使用的内切酶的公司的目录或者网站;2. 用软件如:Primer Premier5.0或Bioedit等,这些软件均提供了内切酶识别序列的信息;3. 推荐到NEB的REBASE数据库去查(网址:/rebase/rebase.html)当你设计好引物,添加上了内切酶识别序列,下一步或许是添加保护碱基了,可以参考:NEB公司网站提供的关于设计PCR引物保护碱基参考表下载(也可见图片)双酶切buffer的选择(MBI、罗氏、NEB、Promega、Takara)再给大家推荐一种新的不需要连接反应的分子克隆方法,优点包括:①设计引物不必考虑选择什么酶切位点;②不必考虑保护碱基的问题;③不必每次都选择合适的酶来酶切质粒制备载体;④而且不需要DNA连接酶;⑤假阳性几率低(因为没有连接反应这一步,载体自连的问题没有了)。
益骨汤对去势大鼠骨密度及经典BMP2信号通路的影响
益骨汤对去势大鼠骨密度及经典BMP2信号通路的影响林晓芳;方芳;李桂锦;姚新苗【期刊名称】《中国现代医生》【年(卷),期】2024(62)6【摘要】目的观察益骨汤对去势大鼠骨组织中经典骨形态发生蛋白(bone morphogenetic protein,BMP)2信号通路的影响,旨在探讨益骨汤防治骨质疏松症(osteoporosis,OP)的作用机制。
方法将44只12周龄雌性Wistar大鼠随机分为四组,每组11只。
正常组不进行任何处理,模型组、戊酸雌二醇组和益骨汤组大鼠进行去势手术以模拟骨质疏松。
造模成功后,分别以10ml/kg双蒸水灌胃(正常组、模型组)、0.36mg/kg戊酸雌二醇水溶液灌胃及10ml/kg益骨汤灌胃,1次/d。
12周后,双能X射线骨密度仪测定股骨骨密度(bone mineral density,BMD),实时聚合酶链反应(polymerase chain reaction,PCR)检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX基因的表达,免疫组织化学法、Western blot法检测BMP2经典信号通路BMP2、Smad1、Runx2、OSX蛋白的表达量。
结果①双能X射线骨密度仪测定股骨骨密度,结果显示,与模型组比较,益骨汤组大鼠的BMD升高(P<0.01)。
②实时荧光定量PCR检测,与模型组比较,益骨汤组大鼠的BMP2、Smad1、Runx2、Noggin基因表达量升高(P<0.05)。
③免疫组织化学法检测:与模型组比较,益骨汤组大鼠的BMP2、Smad1、Runx2、OSX蛋白表达量升高(P<0.05)。
④Western blot法检测:与模型组比较,益骨汤组大鼠的Smad1、Runx2、OSX蛋白含量升高(P<0.05)。
结论益骨汤可能通过激活BMP2经典信号通路及抑制Noggin的高表达,促进骨形成和防治骨质疏松。
【总页数】7页(P73-78)【作者】林晓芳;方芳;李桂锦;姚新苗【作者单位】浙江省台州医院骨科;浙江中医药大学第三临床医学院针灸神经生物学实验室;浙江中医药大学附属第三医院骨科【正文语种】中文【中图分类】R33【相关文献】1.益骨汤对骨质疏松大鼠骨密度及BMP-2信号通路的影响2.益骨汤对去势大鼠骨组织Wnt/β-catenin经典信号通路的影响3.益骨汤对去势大鼠的骨密度、骨生物力学的影响及相关分析4.基于PI3 K/AKT/GSK-3β信号通路探讨益骨汤对去势大鼠骨质疏松的作用5.益骨汤对去势大鼠血清激素水平、骨密度和骨生物力学影响的实验研究因版权原因,仅展示原文概要,查看原文内容请购买。
腹腔镜右半结肠切除同期行肝方叶转移癌切除1例
·7JOURNAL OF RARE AND UNCOMMON DISEASES, MAY. 2024,Vol.31, No.5, Total No.178【第一作者】汪圣毅,男,主任医师,主要研究方向:胃肠疾病。
E-mail:*******************【通讯作者】汪圣毅·论著·腹腔镜右半结肠切除同期行肝方叶转移癌切除1例汪圣毅* 闫 强 张俊义 佘贤忠安徽医科大学第一附属医院普外科 (安徽 合肥 230022)【摘要】目的 分析腹腔镜下同期治疗结肠癌及其肝转移的过程,探讨腹腔镜同期手术在治疗结肠癌肝转移中的作用。
方法 回顾性分析2023年3月安徽医科大学第一附属医院1例结肠癌伴肝方叶转移患者的临床资料,同期行混合入路腹腔镜右半结肠切除、Glisson蒂横断法肝方叶转移癌切除。
结果 R0切除右半结肠及肝转移癌,术中出血量约50 mL,第4天拔引流管,无并发症,顺利出院。
病理结果:结肠溃疡型低分化腺癌,肠周(12/21)个淋巴结有癌转移,低分化肝转移癌。
结论 腹腔镜同期行右半结肠切除和肝转移癌切除的治疗安全可行,采用新的手术路径和操作模式可保证手术安全,值得推广。
【关键词】腹腔镜右半结肠切除术;结肠癌肝转移;腹腔镜肝切除术;Glisson蒂横断法【中图分类号】R656.9; R657.3【文献标识码】ADOI:10.3969/j.issn.1009-3257.2024.5.003Laparoscopic Right Hemicolectomy with Simultaneous Resection of Liver Metastases in the Square Lobe: a Case ReportWang Sheng-yi *, Yan Qiang, ZHang Jun-yi, SHe Xian-zhong.Department of general Surgery, the First affiliated Hospital of anhui Medical University, Hefei 230022, anhui Province, Chinaabstract: objective The process of laparoscopic simultaneous resection of colon cancer and its liver metastasis was analyzed, and the role of laparoscopicconcurrent surgery in the treatment of colon cancer liver metastasis was discussed. Methods The clinical data of one colon cancer patient with square lobe metastasis in the First affiliated Hospital of anhui Medical University in March 2023 were retrospectively analyzed, and laparoscopic right hemicolectomy by mixed surgical approach combined with liver metastasis resection by glisson pedicle transection were performed at the same time. Results R0 resection of colon cancer and its liver metastases was performed with the intraoperative bleeding was about 50 ml. The drainage tube was removed on the 4th day with no complications. The patient was successfully discharged. Pathological results: colon ulcerative poorly differentiated adenocarcinoma, with peri-intestinal metastatic lymph nodes (12/21). liver metastases were poorly differentiated. Conclusion Laparoscopic right hemicolectomy and simultaneous liver metastasis resection is safe and feasible, and the use of new surgical approach and operation methods can ensure the safety, which is worth being popularized.Keywords: Laparoscopic Right Hemicolectomy; Liver Metastases of Colon Cancer; Laparoscopic Hepatectomy; Glisson’s Pedicle Transection Method1 临床资料 患者男,60岁,因“间断性腹痛腹胀20天”入院,腹痛为间断性,大便习惯改变,不成形,无血便,小便正常,消瘦乏力。
dip工作总结报告
dip工作总结报告1. 引言在过去的一段时间里,我在数字图像处理(DIP)领域积极参与了各种项目和任务。
本报告旨在总结我在DIP方面的工作经验和取得的成就,同时分享一些我所学到的知识和技能。
2. 项目一:图像去噪在这个项目中,我们的目标是开发一种有效的图像去噪算法。
我首先对图像噪声的类型和来源进行了调研,然后提出了一种基于小波变换的去噪方法。
通过在不同频率上对图像进行分解和滤波,我们成功地降低了噪声水平,并增强了图像的质量。
该算法在多个实验中得到验证,并取得了令人满意的结果。
3. 项目二:图像分类这个项目要求我们开发一个自动图像分类系统,能够将输入的图像分为不同的类别。
我首先进行了数据集的收集和整理,并对数据进行了预处理,包括图像增强、特征提取等。
然后,我尝试了多种机器学习算法,如支持向量机(SVM)和卷积神经网络(CNN),并对它们的性能进行了比较。
最终,我们采用了深度学习方法,通过训练一个CNN模型,成功实现了准确的图像分类。
4. 项目三:图像分割在这个项目中,我们致力于将输入的图像分割成多个区域,并根据区域的特征进行进一步的分析和处理。
为了达到这个目标,我运用了视觉注意力机制和区域生长算法。
首先,我使用了一种基于显著性检测的方法来获取感兴趣的区域。
然后,我使用区域生长算法将这些区域进一步分割,并对它们进行特征提取和分析。
最终,我们成功地实现了准确的图像分割,并进一步应用于目标检测和识别等任务中。
5. 结论通过参与这些DIP项目,我不仅巩固了我在数字图像处理领域的知识,还学到了许多新的技能和算法。
我深入了解了图像去噪、图像分类和图像分割等关键问题,并成功地应用了各种方法。
在今后的工作中,我将继续努力深化我的DIP技术,探索更多领域的应用,并不断提升自己的能力和水平。
参考文献:[1] Smith, J. et al. (2018). A Wavelet-Based Denoising Algorithm for Digital Images. Journal of Image Processing, 25(3), 112-126.[2] Li, W. et al. (2019). Deep Learning for Image Classification: A Comprehensive Review. Neural Networks, 101, 235-247.[3] Zhou, H. et al. (2020). Region-Based Image Segmentation Using Saliency Detection and Region Growing. IEEE Transactions on Image Processing, 29, 1285-1298.。
medip实验流程
medip实验流程English Response:MeDIP-seq (Methylated DNA Immunoprecipitation sequencing) is an experimental technique used to identify and characterize methylated regions of DNA. It involves immunoprecipitating methylated DNA fragments using an antibody specific to 5-methylcytosine (5mC), followed by sequencing the precipitated DNA.Key steps in the MeDIP-seq workflow:1. DNA fragmentation: Genomic DNA is fragmented using sonication or enzymatic digestion to generate DNA fragments of an appropriate size for immunoprecipitation.2. Immunoprecipitation: Fragmented DNA is incubated with an antibody specific to 5mC. The methylated DNA fragments are immunoprecipitated using magnetic beads or protein A/G beads conjugated to the antibody.3. DNA library preparation: Immunoprecipitated DNA is purified and used to generate a DNA library for sequencing. This typically involves end-repair, dA-tailing, andligation of sequencing adapters.4. Sequencing: The DNA library is sequenced using a next-generation sequencing platform.5. Data analysis: Sequencing reads are aligned to the reference genome, and bioinformatic tools are used to identify methylated regions and analyze methylation patterns.MeDIP-seq provides valuable insights into the epigenetic landscape of the genome. It has been widely used to study DNA methylation in various biological contexts, including development, disease, and environmental exposure.中文回答:MeDIP 实验流程。
限制酶酶切位点
限制酶酶切位点限制酶酶切位点是一种常见的分子生物学技术,用于在DNA或RNA分子中特定的序列上进行切割。
酶切位点是酶识别的特定序列,一旦找到该序列,酶便会在该位置切割DNA或RNA分子。
限制酶酶切位点在基因工程和分子生物学研究中起着至关重要的作用,下面将详细介绍限制酶酶切位点的相关知识。
酶切位点是由限制酶识别的特定DNA或RNA序列决定的。
限制酶是一类能够识别并切割DNA或RNA特定序列的酶,也被称为内切酶。
限制酶通常识别的切割位点是对称的,可以是4-8个碱基对长。
常见的限制酶有EcoRI、HindIII、BamHI等。
限制酶酶切位点的限制酶识别序列通常具有一定的保守性,也就是说,相同的限制酶通常会识别相似的序列。
例如,EcoRI识别的酶切位点序列为5'-GAATTC-3',其中GAATTC为限制酶识别序列,5'和3'分别表示DNA的两个末端。
限制酶酶切位点的限制酶识别序列通常具有一定的特异性,也就是说,相同的限制酶通常只会识别特定的序列。
例如,EcoRI只会识别5'-GAATTC-3'序列,而不会识别其他序列。
限制酶酶切位点的选择对于实验的设计非常重要。
在分子生物学实验中,科研人员常常需要在特定的DNA或RNA序列上进行切割,以便进一步进行下游实验,如限制酶切割后的DNA片段的连接、测序等。
因此,选择适当的限制酶和相应的酶切位点非常关键。
限制酶酶切位点的选择需要考虑多个因素。
首先,需要考虑实验所需的切割位点是否存在于DNA或RNA序列中。
其次,需要考虑限制酶的切割效率和特异性。
一些限制酶可能对特定的序列有更高的切割效率,而对其他序列的切割效率较低。
此外,还需要考虑限制酶的切割位点的位置是否适合实验的要求。
在实际应用中,科研人员常常需要将限制酶切割后的DNA片段进行连接,以构建重组DNA分子。
在这种情况下,选择具有兼容的酶切位点的限制酶非常重要。
兼容的酶切位点是指两个限制酶切割产生的DNA片段的末端具有相同的序列。
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THE INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMPUTER ASSISTED SURGERY Int J Med Robot
Introduction
Computer-assisted surgery (CAS) is a methodology that translates into accurate and reliable image-to-surgical space guidance. Neurosurgery is a very complex procedure and the surgeon has to integrate multi-modal data to produce an optimal surgical plan. Often the lesion of interest is surrounded by vital structures, such as the motor cortex, temporal cortex, vision and audio sensors, etc., and has irregular configurations. Slight damage to such eloquent brain structures can severely impair the patient (1,2). CASMIL, an imageguided neurosurgery toolkit, is being developed to produce optimum plans resulting in minimally invasive surgeries. This system has many innovative features needed by neurosurgeons that are not available in other academic and commercial systems. CASMIL is an integration of various vital modules, such as rigid and non-rigid co-registration (image–image, image–atlas and
SVI像素处理技术
SVI像素处理技术SVI Pro 2009版本代表着地震像素技术的最新发展,是目前最先进的地震像素处理技术,在世界各大石油公司和服务公司得到了广泛的应用。
一、地震像素处理技术概述地震解释与属性应用技术在最近的20年中得到了飞速的发展。
从1983年出现第一台地震解释工作站开始,地震解释技术大致经历了三维地震解释技术发展、地震可视化技术发展、地震属性技术发展;最近,又出现了三维地震像素处理技术,利用图像处理技术,结合地震属性处理技术,解决复杂的地质问题。
三维地震像素处理技术尤其适合复杂地质条件下的构造解释与描述、油气储集体探测和描述、复杂断层体系的自动探测和描述等。
SVI Pro是英国ffA公司多年研发的成果,代表着三维地震像素处理的最新进展。
那么,什么是像素处理和地震像素呢?其目的是什么呢?上世纪60年代后由于对空间和深海洋底的探索需求,以及冷战中对高空侦察照像图件的处理需求,图像处理技术得到了很大的发展。
在空间和洋底的研究中,面临着大量模糊的图像,包括二维图像和三维图像。
如何在一定的条件下从这些模糊的图像中最大限度的提取有用信息,就成了相关科技人员所面临的挑战。
针对这种需求,图像处理技术得到了很大的发展来应对这种图像处理技术需求,像素处理技术逐步得到了完善,并发展成一个完整的学科体系,在空间技术和海洋科学的发展中起着很大的作用,在军事上更是不可缺少的关键技术之一。
目前,图像处理技术已经深入到了我们生活中的很大一部分,我们周围的很多事物中都显性或隐性的应用着图像处理技术:空间探索、海洋探索、军事侦察、医疗成像、结构探伤、卫片处理、气象成图、资源勘探、敌我识别系统、导弹自动识别制导、汽车主动安全系统等等。
像素处理技术是图像处理技术中的核心,如果对此技术有兴趣进一步了解详情,可参考John C. Russ著的图像处理手册(Image Processing Handbook)。
现代成图设备大都可以生成数字图像,数字图像由二维光栅(pixels)或三维光栅(voxels)所组成。
EP9A2-中文
用患者样本进行方法比对及偏倚评估批准指南 -- 第二版这个文件介绍的程序供两个临床方法间的偏倚评估及采用一分为二的患者标本和资料数据的方法学比较实验设计。
通过NCCLSA同过程制定全球应用的指南VNCCLSNCCLS •…通过自愿认同的方式服务于全世界医学科学团体NCCLS是一个国际性、多学科、非盈利、制定标准、教育型组织,在临床检验界内促进标准和指南的使用和发展。
为患者检验和相关临床检验组织制定标准和指南在全球范围内得到一致认同过程。
NCCLSliJ定标准的原则是从质量效益和成本效益两方面考虑,为患者检验和临床检验组织服务。
除了制定和促进标准、指南的推广外,NCCLSS提供了一个开放、无偏见的论坛,供发表影响患者检验和临床检验质量的批评性言论。
出版物NCCLSC件以标准、指南和委员会报告出版。
标准:通过认同过程形成文件,对材料、方法或不能修改的实践方式明确规定其特定的基本要求。
另外,标准也可以包含明确规定的选定要素。
指南:通过认同过程形成文件,制定常规实验操作程序、方法或材料的规范。
使用者可以使用或修改指南以满足特定的需要。
报告:没有经过认同过程,由理事会颁布。
认同过程NCCL弘同过程,建立正式规范的程序如下:1. 项目的授权;2. 文件的制定和公开评审;3. 根据使用者的反馈评论修订文件;4. 文件被接受为标准或指南。
大多数NCCLSC件只有“建议”和“批准”两个层次的认同过程,根据领域评价或资料收集的需要,文件也可以有中间(如“试行”)认同层次。
建议:作为NCCLS勺建议标准或指南处在被临床检验界评审的第一阶段,此文件需接受广泛、全面的技术审核,包括对范围、方法、用途及技术和编写内容的逐行逐字的审核。
试行:当一种推荐方法对某一领域的评审有明确的需要或者当某一建议性方案需要收集特定的数据时,才制定试行标准或指南。
同样,试行标准或指南也应接受审核,以保证其有效性。
批准:批准的标准或指南已在临床检验界得到认同。
高分辨率溶解曲线.pptx
Rosario Muleo,Marir Chiara Colao,Dario Miano at el. 2009 Genome52:252-260
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Mutation Scanning
未知SNP扫描
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等对北京油鸡CAPN1基因外显子进行扫描,筛查其潜在SNPs。
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Bernhard等对Barley的eukaryotic translation
initiation factor 4E (eIF4E) gene基因外显子进行突变扫描结果
— 筛查突变 (mutation scanning) — 基因分型(Mutation Genotyping)
—小片段+内标法( Small Amplicon Genotyping ) —非标记探针法(Unlabeled Probe Genotyping,LunaProbe) —SSR分析 — 检测甲基化 • HRM仪器
内容提示
• 高分辨熔解曲线(High Resolution Melting) • 高分辨熔解曲线在遗传育种中的应用
— 筛查突变 (mutation scanning) — 基因分型(Mutation Genotyping)
—小片段+内标法( Small Amplicon Genotyping ) —非标记探针法(Unlabeled Probe Genotyping,LunaProbe) —SSR分析 — 检测甲基化 • HRM仪器
• 0.3oC/秒 升温速度= 67 pts/ ℃ • 0.05oC/秒 升温速度 = 400 pts/ ℃
用于预测结直肠癌术后复发与转移的检测组合物、检测装置、检测试
专利名称:用于预测结直肠癌术后复发与转移的检测组合物、检测装置、检测试剂盒及应用
专利类型:发明专利
发明人:陈兴,韩梦婷,黄蓉冰
申请号:CN201910101233.3
申请日:20190131
公开号:CN111500715A
公开日:
20200807
专利内容由知识产权出版社提供
摘要:本发明公开了一种用于预测结直肠癌术后复发与转移的检测组合物、检测装置、检测试剂盒及应用。
该检测组合物包括用于检测OGA外显子11可变剪切发生情况的试剂。
本发明的发明人首次发现了OGA的一种新的剪切异构体tOGA,形成tOGA剪切异构体的外显子11的可变剪切(外显子跳读)受到O‑GlcNAc修饰的显著调控。
对于术前未进行过化疗的结直肠癌患者,该标志物在其肿瘤组织中相对于癌旁组织和正常肠组织中具有明显差异,而且该标志物与肿瘤的复发与转移具有相关性,因此,OGA外显子11可变剪切(外显子跳读)的发生情况可以作为预测结直肠癌术后复发与转移的分子标志物。
申请人:北京大学
地址:100871 北京市海淀区成府路202号
国籍:CN
代理机构:北京康信知识产权代理有限责任公司
代理人:韩建伟
更多信息请下载全文后查看。
垭紫罗断裂带深部构造分段特征及构造变换作用
垭紫罗断裂带深部构造分段特征及构造变换作用汪新伟;郭彤楼;沃玉进;周雁;吴莉芝;张荣强;李双建【摘要】垭紫罗断裂带深部构造分段特征及构造变换的研究对其两侧盆地的构造化演化、沉积充填、油气成藏的差异分析均有重要意义.运用重、磁、电、震等资料的综合分析表明,垭紫罗断裂带走向上可分为3段,具有由西北段(威宁段)的4条深断层转换为中段(关岭段)2条深断层、至东南段(紫云段)时演变为1条深断层的构造变换特征,且正断层的主体倾向亦由WS倾变换为NE倾;同时,该断裂带对古生代沉积具有由西北段向东南段迁移的控制作用,即西北段控制了志留纪槽盆的沉积范围,西北段至中段则限定了泥盆系向黔中隆起的超覆边界与石炭纪深水槽盆沉积区,东南段则为二叠纪深水沉积带.垭紫罗断裂带中段(关岭段)的构造演化经历了泥盆纪初始伸展期、石炭纪断陷期、二叠纪裂谷期、三叠纪构造反转期和侏罗纪以来的陆内收缩期等5个阶段,其变形样式表现为晚古生代地堑的边界断层发生反转而形成的扇形背冲结构,构造剖面的缩短率高达51.68%.%The study on deep structural segmentation and transformation of the Yaziluo fault zone is of great significance for understanding the tectonic evolution,sedimentation and hydrocarbon accumulation of the basins on its two sides.Based on comprehensive analyses of gravity,magnetic,electric and seismic data,this paper divided the Yaziluo fault zone into three segments along its strike.The structural transformation is characterized by four deep faults in the northwest segment (Weining segment),two deep faults in the middle segment (Guanling segment),and one deep fault in the southeast segment (Ziyun segment).The major dip direction of the normal faults changes from southwestward to northeastward.Meanwhile,the Yaziluo fault zonecontrols the migration of the Paleozoic deposits from the northwest segment to the southeast segment,i,e.the northwest segment controls the limit of the Silurian deposition,the northwest to the middle segments control the Devonian onlap boundary to the Qianzhong uplift and the Carboniferous deep basin sedimentation,and the southeast segment controls the Permian deep-water sedimentary belt.The tectonic evolution of the middle segment (Guanling segment) of the Yaziluo fault zone experienced five stages,namely the initial extension during the Devonian,the extensional faulting during the Carboniferous,the rifting during the Permian,the tectonic inversion during the Triassic and the inland contraction since the Jurassic.Its deformation style is characterized by a fan-shaped back thrusting structure resulted from reversing of the boundary faults of the Late Paleozoic graben.The shortening rate of structural profile is up to 51.68 %.【期刊名称】《石油与天然气地质》【年(卷),期】2013(034)002【总页数】9页(P220-228)【关键词】分段特征;构造变换;深部构造;垭紫罗断裂带【作者】汪新伟;郭彤楼;沃玉进;周雁;吴莉芝;张荣强;李双建【作者单位】中国石化石油勘探开发研究院,北京100083;中国石化南方勘探开发分公司,四川成都610041;中国石化石油勘探开发研究院,北京100083;中国石化石油勘探开发研究院,北京100083;中国石化中原油田分公司勘探开发研究院,河南濮阳457001;中国石化石油勘探开发研究院,北京100083;中国石化石油勘探开发研究院,北京100083【正文语种】中文【中图分类】TE121.2图1 垭紫罗断裂带及邻区构造纲要Fig.1 Structural outline map of the Yaziluo fault zone and its adjacent areaF1.遵义断层;F2.赫章-金沙断层;F3.纳雍-息峰断层;F4.贵阳-施秉断层;F5.六盘水北断层;F6.威宁断层;F7.垭都-蟒洞断层;F8.六盘水断层;F9.紫云-罗甸断层;F10.块择河断层;F11.兴仁西断层;F12.贞丰断层;F13.南盘江断层;F14.南丹断层;F15.河池-宜州断层垭紫罗断裂带(即垭都-紫云-罗甸断裂带,下同)是扬子板块南部的一条重要的北西向构造带,长约400 km、宽10~80 km,分隔了两侧的黔西北坳陷、黔中隆起、黔南坳陷与滇东北冲断褶皱区、黔西南坳陷、南盘江坳陷等(图1)。
表面处理与电镀英语
电镀专业术语中英文对A篇1 大气暴露试验atmospheric corrosion test2 中性盐雾试验(NSS试验) neutral salt spray test(NSS-test)3 不连续水膜water break4 pH计 pH meter5 孔隙率 porosity6 内应力 internal stress7 电导仪 conductivity gauge8 库仑计(电量计) coulomb meter9 旋转圆盘电极 rotating disk electrode10 旋转环盘电极 rotating ring disk electrode11 针孔 pores12 铜加速盐雾试验(CASS试验) copper accelerated salt spray (CASS test ).13 参比电极 reference electrode14 甘汞电极 calomel electrode15 可焊性 solder ability16 硬度 hardness17 金属变色 tarnish18 点滴腐蚀试验 dropping corrosion test19 玻璃电极glass electode20 结合力 adhesion21 哈林槽 Haring cell22 恒电势法 potentiostatic method23 恒电流法 galvanostatic method24 交流电流法 a.c method25 树枝状结晶 trees26 脆性 brittleness27 起皮 peeling28 起泡 blister29 剥离 spalling30 桔皮 orange peel镀(Plating)电镀(Electroplating)自催化镀(Auto-catalytic Plating)化学镀(Chemical Plating)无电镀(Electroless Plating)浸渍镀(Immersion Plating)阳极氧化(Anodizing)化学转化层(Chemical Conversion Coating)钢铁发蓝(Blackening),俗称”发黑“钢铁磷化(Phosphating)铬酸盐处理(Chromating)金属染色(Metal Colouring)涂装(Paint Finishing),包括各种涂装如手工涂装、静电涂装、电泳涂装等热浸镀(Hot dip)热浸镀锌(Galvanizing),俗称"铅水"热浸镀锡(Tinning)PVD 物理气相沈积法(Physical Vapor Deposition)真空镀(Vacuum Plating)离子镀(Ion Plating)CVD 化学气相沈积法(Chemical Vapor Deposition)ABS塑料电镀 plastic plating processpH计 pH meter 测定溶液pH值的仪器。
舌癌组织中OPN、Pyk2、ERK、MMP-9的表达情况及其临床意义
舌癌组织中OPN、Pyk2、ERK、MMP-9的表达情况及其临床意义黄小芳;李玉宁;李文鹿;郭琼【期刊名称】《实用癌症杂志》【年(卷),期】2024(39)5【摘要】目的分析骨桥蛋白(OPN)、富含脯氨酸的酪氨酸激酶2(Pyk2)、细胞外信号调节激酶(ERK)、基质金属蛋白酶-9(MMP-9)在舌癌患者癌组织中的表达及其临床意义。
方法选取62例舌癌患者,收集其癌组织与癌旁正常组织(距离病灶边缘≥3 cm),分别纳入观察组与对照组,对比2组OPN、Pyk2、ERK、MMP-9的差异。
另收集患者的年龄、性别等资料,分析OPN、Pyk2、ERK、MMP-9与患者各项临床病理特征的关系。
结果观察组OPN、Pyk2、ERK、MMP-9阳性表达率高于对照组,差异有统计学意义(P<0.05)。
OPN、Pyk2、ERK、MMP-9表达与舌癌患者的年龄、性别、肿瘤直径无关(P>0.05),与临床分期、分化程度、淋巴结转移有关(P<0.05)。
结论OPN、Pyk2、ERK、MMP-9在舌癌患者的癌组织内呈异常的高表达,且与肿瘤的临床分期、分化程度、淋巴结转移具有紧密联系。
【总页数】3页(P710-712)【作者】黄小芳;李玉宁;李文鹿;郭琼【作者单位】郑州大学第一附属医院郑东院区【正文语种】中文【中图分类】R739.86【相关文献】1.鼻咽癌组织中 ERK1/2、p-ERK1/2和 MMP-9蛋白的表达变化及其相关性2.MMP-9、ERK与PTEN在舌癌组织中的表达及临床意义3.三阴性乳腺癌组织MMP-9、PD-L1及OPN的表达情况及相关性分析4.OPN、Pyk2、p-AKT在舌鳞状细胞癌中的表达及临床意义因版权原因,仅展示原文概要,查看原文内容请购买。
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a Hough Transform pair. A point in image space and a line in parameter space are a Hough Transform pair.
Original space Parameter Space
yi axi b
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x,y p
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Hough Transform
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Hough Transform
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Hough Transform
Hough Transform: 20 colinear points
•p, θ representation of line •Maximum accumulator value is 20
Edge Image (Canny)
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Hough Transform
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Hough transform detecting circles
Although the focus so far has been on straight lines, the hough transform is applicable to any function of the form g(v,c)=0, where v is a vector of coordinates and c is a vector of coefficients. For example, the points lying on the circle
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Hough Transform
1. They belonged to one of the three accumulator cells with the highest count 2. No gaps were longer than five pixels
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( x c1 ) ( y c2 ) c
2 2
2 3
can be detected by using the approach just discussed. The only difference is that there are three parameter.
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Hough Transform
a can be huge for near vertical lines, and there is no representation for a vertical line. Solution – using polar representation for lines.
Hough transform detecting circles
The procedure is to increment c1 and c2, solve for the c3 that satisfy:
( x c1 ) ( y c2 ) c
2 2
2 3
Then updated the accumulator corresponding to the cell associated with the triplet (c1,c2,c3) Clearly, the complexity of the hough transform is proportional to the number of coordinates and coefficients in a given functional representation
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Hough Transform
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Hough Transform
Quantize the parameter space (an array) and let the generation of a line be the increment of a counter at all cells in the parameter space that lie along the line. Histogram the resulting parameter space (accumulators). Peaks in this accumulator array occur at parameter locations corresponding to lines with significant support.
*A solution: Transform
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Hough Transform
yi axi b y j ax j b
Original space
Hough 1962
Hough Transform
b xi a yi b x ja y j
Parameter Space
b xi a yi
' ' '
yi axi b
b x ja y j
'
yi xi a b
' '
' '
y j x ja b
Points-Lines Duality
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Hough Transform
Consider a straight line. The HT maps all the points on the line onto a single point in the parameter space. A line in image space and a point in parameter space are
Eliminates isolated short segments, link little breaks.
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Global Processing
Global Processing Find lines, curves, or parts of lines or curves in an input image.
– Edge linking and boundary detection – Thresholding – Region based segmentation
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Edge Link and Boundary Detection
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Local Processing
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Hough transform detecting circles
Hough Transform
Note: the parametric solution represents the whole line and not the segment
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Hough Transform
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Hough Transform
Gray Scale Image
Local Processing
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Local Processing
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Local Processing
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Local Processing
Use vertical and horizontal Sobel operators Link conditions: gradient value > 25; gradient direction difference < 15
Hough Transform
Calculating steps of hough transform: 1. In parameter space, quantize a and b, and give out (amin,amax) and (bmin,bmax). 2. Set an accumulator A: A is A(amin:amax, bmin:bmax),set A to zero at the beginning. 3. For a given point (xi,yi) in the original space, let a equal to each allowed values on the aaxis, and solve for the corresponding b using the equation: b xi a yi 4. The resulting b are then rounded off to the nearest allowed value in the b-axis. If a choice of ap results in solution bq, we let A(p,q)=A(p,q)+1 5. At the end of this procedure, a value Q in A(i,j) corresponds to Q points in the xy space lying on the line y=aix+bj.
Digital Image Processing
Image Segmentation
Luguangm@
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Contents
Today we will begin looking at automated image analysis by examining the thorny issue of image segmentation:
x cos y sin
In polar representation, a point in the image correspond to sinusoidal curve in the parameter space x cos <=90 y sin -90<=