慢性脑缺血小鼠脑血管形态学变化的活体动态观察

慢性脑缺血小鼠脑血管形态学变化的活体动态观察
楠迪;郭淮莲;何其华;宋江曼
【期刊名称】《中国卒中杂志》
【年(卷),期】2015(000)002
【摘要】目的通过建立慢性脑缺血模型，活体动态观察慢性脑缺血后小鼠脑血管
形态学变化。

n<br> 方法 Tie2-GFP转基因小鼠7只，左侧颈总动脉永久性
结扎结合右侧颈总动脉短暂结扎方法制备慢性脑缺血模型，分别在脑缺血前（D0)和脑缺血后7 d（D7），利用颅窗和激光共聚焦显微镜活体观察小鼠脑缺血前后
皮层毛细血管、细动脉及细静脉的变化情况。

n<br> 结果①缺血前和脑缺血
后7 d毛细血管直径分别是（6.62±0.75）μm和（12.50±3.29）μm，脑缺血后
毛细血管直径较缺血前扩张（P＜0.001）；②脑缺血前和脑缺血后7 d毛细血管
密度分别为每感兴趣区域（region of interest，ROI）（11.67±1.72）个和每ROI（11.08±2.06）个，差异无显著性（P=0.0583）；③脑缺血前和脑缺血后7 d分支毛细血管、网状毛细血管、集合毛细血管及细动静脉直通支直径分别为（6.33±0.94）μmvs （12.36±3.20）μm，（6.87±1.10）μmvs （12.37±2.78）μm，（6.37±0.52）μmvs （11.41±3.10）μm及（6.35±0.92）μmvs
（13.91±6.17）μm，脑缺血后均扩张（P分别为＜0.01，＜0.01，＜0.01及＜0.05），其中细动静脉直通支扩张最显著；④细静脉直径在D0和D7时分别为（19.59±8.74）μm和（24.81±6.25）μm，脑缺血后细静脉显著扩张
（P=0.0054）；而细动脉直径在D0和D7时分别是（12.71±2.10）μm和（13.20±3.09）μm，无显著性改变（P=0.2947）；⑤可见细动静脉迂曲、吻合
支开放及毛细血管走行改变等血管重构现象。

n<br> 结论慢性脑缺血后皮层
血管发生毛细血管扩张、细静脉扩张等改变，这些改变可能有助于调节脑血流量。

%Objective To dynamically observe the morphological changes of the cortical vessel in mice after chronic cerebral hypoperfusion through a cranial window. n<br> Methods The left common carotid artery ligation and transient right common carotid artery ligation operations were performed on 7 Tie2-GFP transgenic mice. The cortical vessels were observed using confocol microscopy through a cranial window before brain ischemia (D0) and 7 days after brain ischemia (D7). The diameter and density of capillaries, along with the diameter and morphological change of arterioles and venules, were analyzed. n<br> R esults①The diameters of capillary before and 7 days after the brain ischemia were (6.62±0.75)μm and (12.50±3.29)μm, which increased significantly after brain ischemia (P<0.001).②The capillary densities before and 7 days after the brain ischemia were (11.67±1.72) segments per region of interest (ROI) and (11.08±2.06) segments per ROI. The difference was not signiifcant
(P=0.0583).③The diameters of the precapillary arteriole, post capillary venule, true capillary and thoroughfare channel before and 7 days after the brain ischemia were (6.33±0.94)μmvs (12.36±3.20)μm, (6.87±1.10)μmvs
(12.37±2.78)μm, (6.37±0.52)μmvs (11.41±3.10)μm and (6.35±0.92)μm vs
(13.91±6.17)μm, respectively, all increased significantly after brain ischemia (P<0.01, <0.01,<0.01, and <0.05, respectively). The most signiifcant dilation was observed in thoroughfare channel.④The diameters of venules on D0 and D7 were (19.59±8.74)μm and (24.81±6.25)μm, which increased signiifcantly after brain ischemia (P=0.0054). However, the diameters of
arterio les on D0 and D7 were (12.71±2.10)μm and (13.20±3.09)μm, the difference was not signiifcant (P=0.2947);⑤Capillary remodeling, tortuosity and new anastomosis between venules were observed after brain ischemia. n<br> Conclusion The dilation of capillaries and venules were observed after brain ischemia. These changes may contribute to the regulation of cerebral blood flow after chronic cerebral hypoperfusion.
【总页数】6页(P107-112)
【作者】楠迪;郭淮莲;何其华;宋江曼
【作者单位】100044 北京北京大学人民医院神经内科;100044 北京北京大学人民医院神经内科;北京大学医药卫生分析中心;100044 北京北京大学人民医院神经内科
【正文语种】中文
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