热疗增强长春瑞滨对人肺癌PLA -801D细胞株毒性的实验研究

热疗增强长春瑞滨对人肺癌PLA -801D细胞株毒性的实验研
究
孙胜杰;吴志勇;焦顺昌
【期刊名称】《中国药物应用与监测》
【年(卷),期】2012(009)002
【摘要】Objective: To investigate the sensitivity effect of hyperthermia on Vinorelbine and its mechanism. Methods: Growth inhibition rates of human pulmonary carcinoma cells (PLA-801D) in single thermotherapy group, single chemotherapy group and hyperthermia-chemical combination treatment group were determined by MTT assay. Changes in cell cycle after treatment at 42 ℃ were analyzed by flow cytometry (FCM). Results: Hyperthermia at 42 -43℃ suppressed and killed the proliferation of PLA- 801D cells (P < 0.001). However, hyperthermia at 38 - 41 ℃ accelerated the proliferation of pulmonary carcinoma cells (P < 0.001). Hyperthermia combination with drugs had the best effective results and there was positive correlation between the inhibition rate and temperature changes (r=0.921 7, P < 0.01). Cell cycle analysis showed that the thermotherapy and chemotherapy combination group had the greatest change in the amplitude of cell cycles. Conclusion: Hyperthermia should be applied in combination with chemotherapy. Within a certain range, there was positive correlation between the effect of hyperthermia and the high temperature.%目的:探讨不同温度下的温热对长春瑞滨增敏的规律及机制.方法:应
用四氮唑蓝(MTT)快速比色法测定不同温度下的热疗组、单纯化疗组以及以不同序贯方式结合的热化疗组对人肺巨细胞癌细胞( PLA - 801D)的生长抑制率；流式细
胞仪测定42℃时各组细胞周期的变化.结果:38 ～41℃的单纯热疗促进了肺癌细胞的生长(P＜0.001 );42～43℃的单纯热疗对肺癌细胞具有抑制和杀伤作用(P＜
0.001)；温热与药物同时作用效果最佳(P＜ 0.001),且抑制率与温度的变化呈正相
关(r=0.921 7,P＜0.01)；细胞周期分析发现热化同时组细胞各个周期增减的幅度
最大.结论:临床热疗不宜单独进行,需与化疗联合应用；在一定范围内,温度越高,热
化疗的疗效越好.
【总页数】5页(P87-91)
【作者】孙胜杰;吴志勇;焦顺昌
【作者单位】解放军总医院肿瘤内科,北京100853;解放军总医院肿瘤内科,北京100853;解放军总医院肿瘤内科,北京100853
【正文语种】中文
【中图分类】R96
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