7,8-二羟基黄酮对左旋多巴诱导的异动症大鼠行为的影响及其作用机制的探讨

7,8-二羟基黄酮对左旋多巴诱导的异动症大鼠行为的影响及其
作用机制的探讨
赵君焱;甄体丽;舒海洋;黄译腺;刘春风;罗蔚锋
【期刊名称】《临床神经病学杂志》
【年(卷),期】2017(30)6
【摘要】Objective To observe effect of 7,8-dihydroxyflavone (7,8-DHF) on the behaviors of rats with L-DOPA induced dyskinesia and its mechanism .Methods Parkinson's disease ( PD) rat model was established by destroying the right medial forebrain with 6-hydroxydopamine (6-OHDA) on SD rats.Forty successful PD rats were randomly separated into 4 groups:Nacl solution ( NS) group, L-DOPA group, L-DOPA+7,8-DHF group and 7,8-DHF group,and each group had 10 rats.Behavioral tests of abnormal involuntary movement ( AIM) and forepaw adjusting steps were recorded on 2 d, 9 d, 11 d, 18 d and 21 d.The protein expression level of PSD-95 in striatal tissues were detected by Western blotting .Results (1) Compared with those of L-DOPA group, axial, limb, orolingual and total AIM scores of L-DOPA+7,8-DHF group were significantly decreased on 9 d, 11 d, 18 d and 21 d(all P<0.05).(2)The forepaw adjusting steps had no significant difference between L-DOPA+7,8-DHF group and L-DOPA group(all P>0.05).(3) Compared with NS group, PSD-95 protein level up-regulated in the lesioned striatum of L-DOPA group.However, those of L-DOPA +7,8-DHF group and 7,8-DHF group were significantly decreased,
compared with L-DOPA group (all P<0.05).Conclusions 7,8-DHF can ameliorate abnormal involtmtary movements induced by L-DOPA, and does not affect the efficacy of alleviating forepaw adjusting steps by L-DOPA on PD rats.This mechanism may be related to 7,8-DHF suppressed the protein PSD-95 in striatum.%目的探讨7,8-二羟基黄酮(7,8-DHF)对左旋多巴(L-DOPA)诱发的异动症大鼠行为的影响及其作用机制.方法 SD大鼠于右内侧前脑束立体定向注射6-羟基多巴(6-OHDA),建立单侧毁损帕金森病(PD)大鼠模型.成功构建PD模型的40只大鼠随机分为生理盐水(NS)、L-DOPA、L-DOPA+7,8-DHF和7,8-DHF 4个组,每组10只.实验大鼠在给药治疗的第2、9、11、18、21 d进行异常不自主运动(AIM)、毁损对侧前肢跨步数行为学测试.采用Western bloting技术检测实验大鼠脑纹状体组织中蛋白PSD-95的含量.结果 (1)L-DOPA+7,8-DHF组第9、11、18、21 d轴性、肢体、口舌及总的AIM评分较L-DOPA组显著下降(均P<0.05).(2)L-DOPA+7,8-DHF组治疗前后左前肢跨步数较L-DOPA组无明显差异(均P>0.05).(3)L-DOPA组大鼠毁损侧纹状体内PSD-95表达水平较NS组增加,而L-DOPA+7,8-DHF组及7,8-DHF组大鼠毁损侧纹状体内PSD-95蛋白表达水平较L-DOPA组显著下降(均P<0.05).结论 7,8-DHF能够改善L-DO-PA诱导的异常不自主运动,对L-DOPA改善PD大鼠左前肢运动功能的作用并无影响.其机制可能与7,8-DHF降低异动症大鼠纹状体组织中突触后致密物蛋白PSD-95含量有关.
【总页数】5页(P450-454)
【作者】赵君焱;甄体丽;舒海洋;黄译腺;刘春风;罗蔚锋
【作者单位】215004 苏州大学附属第二医院神经内科;215004 苏州大学附属第二医院神经内科;215004 苏州大学附属第二医院神经内科;215004 苏州大学附属第
二医院神经内科;215004 苏州大学附属第二医院神经内科;215004 苏州大学附属第二医院神经内科
【正文语种】中文
【中图分类】R742.5
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