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大动脉炎患者外周血单个核细胞RT-qPCR内参基因的选择

January 2021Vol.41 No.12021 年 1 月第 41 卷第 1 期基础医学与临床Basic & Clinical Medicine文章编号：1001-6325 ( 2021 ) 01-0087-06研究论文大动脉炎患者外周血单个核细胞RT-qPCR 内参基因的选择田苡箫，李菁*收稿日期：2019-11-18 修回日期：2020-04-30*通信作者(corresponding author ) ：lijing6515@ （中国医学科学院北京协和医学院北京协和医院风湿免疫科风湿免疫病学教育部重点实验室国家皮肤与免疫疾病临床医学研究中心，北京100032）扌摘要：目的筛选适于在大动脉炎（TAK ）患者和健康人群（HC ）之间比较外周血单个核细胞（PBMC ）中mRNA 表达水平的内参基因。

方法提取PBMC 中的总RNA,应用RT-qPCR ，分别采用geNorm 、NormFinder 、BestKeeper 3种软件程序，分析 3-glucuronidase ,GAPDH ,ACTB ,SDHA ,HPRT1,RPL13A ,B2M , YWHAZ 和 PKG1 9 个基因的 mRNA 表达稳定性。

以T-bet 、GATA3和RORC 作为目的基因，比较不同稳定性的内参基因对mRNA 相对丰度的影响。

结果geNorm 筛选得到的基因组合为B 2M-SDHA , Nor^nFinder 和BestKeeper 筛选出最稳定的内参基因均为HPRT1 ； 3种方法均显示GAPDH 的稳定性较差。

结论自身免疫病患者在接受免疫抑制药物治疗时,原本稳定表达的基因可能会上调或下调;在样本量较小时，稳定性更好的内参基因可能更有助于检测组间差异。

关键词：大动脉炎;实时定量聚合酶链式反应;RNA 稳定性；内参基因选择中图分类号:R593.2 文献标志码:AValidation of reference genes for the normalization of the RT-qPCRin peripheral blood mononuclear cells of patients with Takayasu arteritisTIAN Yi-xiao ， LI Jing *(Department of Rheumatology and Immunology , Key Laboratory of Rheumatology and Clinical Immunology , Ministry of Education ,National Clinical Research Center for Dermatologic and Immunologic Diseases ( NCRC-DID ),Peking Union Medical College Hospital , CAMS & PUMC , Beijing 100032, China)Abstract ： Objective To validate proper reference genes for quantitative real-time polymerase chain reaction ( RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis" (TAK) and healthy controls' ( HC ) pe ripheral blood mononuclear cells ( PBMC ). Methods Total RNA in PBMCs was extracted and used RT-qPCR to determine the profiles of 9 candidate genes , including 0-glucuronidase, GAPDH , ACTB , SDHA , HPRT1, RPL13A , B2M , YWHAZ and PKG1. Then compared their transcription stability by geNorm , NormFinder , and Best Keeper. Afterwards , with T-bet , GATA3 and RORC as the targeted genes , explored the influence of reference genes with different stability on mRNA relative abundance. Results The gene combination of B2M-SDHA was selected bygeNorm , and HPRT1 was the most stable one in analysis results of NormFinder and BestKeeper , while GAPDH was less stable. Conclusions Genes that have been expressed stably may be upregulated or downregulated whenpatients with autoimmune diseases received immunosuppressive drugs. When the sample size is small ， the more sta ble internal reference may facilitate the identification of inter-groups difference.Key words : Takayasu arteritis ； real-time polymerase chain reaction ； RNA stability ； selection of reference gene88基础医学与临床Basic&Clinical Medicine2021.41(1)反转录实时荧光定量聚合酶链式反应（reverse quantitative real-time polymerase chain reaction,RT-qPCR）是目前分析基因表达水平的黄金标准，却经常表现出重复性欠佳的问题，选取合适的内参基因有助于改善这一情况［1］。

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1.4211.102704 4.20.05043 1.2040.752509 4.90.06046 1.3951.081641 6.30.06074 1.595 1.0497097.40.09638 1.3 1.145413 3.10.04017 1.716 1.55207.60.004652.345 1.049224 1.60.00601 1.352 1.3771389.20.01265 1.752 0.577300 5.50.03426 1.347 0.474403 5.20.03177 1.297 0.723166 2.90.28453 1.339 0.7237333.50.053380.912 0.595289 6.30.017020.7660.82814997.40.127630.9641.0763438.40.03476 1.516 0.597626 6.80.070850.9630.622110470.09860.9211.0453772.70.02083 1.243>10.00.00011 1.506 1.09912429.90.13784 1.05 0.8981353 2.50.05461 1.584 0.3182260.0032 1.1690.75869480.0249 1.0571.25>10.00.00172 1.053 0.711936 6.50.22306 1.179 0.706201 4.70.01384 1.168 0.7392618.50.02793 1.196 0.5911753 3.30.059170.7180.711228 5.80.02249 1.0541.013827 6.90.067680.830.819337 4.30.03418 1.204 0.5245527.10.045390.929 0.235177.20.001430.864 0.355904 5.50.057310.801 0.404171 1.90.004220.839 0.8441018 2.90.021720.749 0.831492 5.50.077660.883 0.447854 5.10.05450.81 0.68921970.02114 1.039 0.702967 4.10.062970.967 0.7151689 6.70.2465 1.159 0.8941061 3.90.051860.961 0.69669 1.40.00158 1.21405>10.00.00072 1.57 0.7321483 4.70.079720.839 0.64835 4.90.028380.744 0.91231440.11162 1.241 0.70896 2.90.005410.609 0.964197 4.70.009170.739 0.28257.30.002590.91611980.00341 1.67 0.825234 5.40.011580.756 0.716524 4.20.021180.7390.43848 4.40.002050.9771.4250 1.50.000460.805 0.71410 4.50.04139 1.1870 4.50.008370.668 0.45566870.04130.919 0.793179 1.30.00205 1.044 0.635222 6.80.015560.791 0.484672 3.50.026580.705 0.766789 4.80.049630.8180.424413 6.90.029650.6261.018111 5.60.01044 1.028 0.287669 3.80.015170.929 0.796368 5.20.03430.799 0.20986 3.20.001390.839 0.58143 6.50.013621 0.441195 5.40.009240.65 0.795171 1.60.001670.422 0.6142647.70.010820.673 0.21414>10.00.004512.295 0.734943.90.002830.6860.765226 3.30.014480.8530.4644018.60.025550.6821.052213 4.90.013750.659 0.5487>10.00.01419 1.433 0.6410467.80.070860.835 0.47215 5.80.008280.868 0.6764449.50.023950.793 0.605583 5.20.036290.663 0.310>10.00.00060.798 0.686185 5.30.005860.592 0.5226 4.60.008520.479 0.588119180.085760.724 0.344756 6.80.024870.586 0.475524>10.00.034330.912 0.276105 3.30.00338 1.185 0.46378 3.80.026420.746 0.602487 5.90.028250.647 0.496280 6.20.034720.765 0.5991610 6.30.124690.81 0.402326 5.90.013060.799 0.52978550.057240.716 0.526409>10.00.020090.672 0.3015487.20.038990.75 0.64937 1.90.000820.528362 5.80.018750.583 0.495103 3.30.005940.555200 4.80.011950.645 0.36816487.70.106370.722 0.591164 1.10.000580.7 0.56342660.032820.763 0.928139>10.00.014250.916 0.574183 5.70.0090.576 0.26453 3.80.026470.7 0.59358360.04060.66 0.51817349.50.098910.574 0.33328870.023130.591 0.29421100.028010.852 0.14854>10.00.004550.917 0.533757.10.00310.519 0.237152 5.20.014030.848 0.3339 4.70.000610.947 0.684358 6.80.01790.68 0.205611 2.60.01090.6360.62216 4.70.011730.744 0.56760 5.20.00460.935 0.5051516 5.10.073520.5710.402102 4.70.008450.7241.286112 1.70.002350.689 0.491962.10.002940.478 0.311617.50.00190.52 0.2641407.50.007770.652 0.623151 5.60.009150.618 0.30427650.011510.675 0.5575198.10.032360.622 0.531271 6.80.01050.542 0.3713267.50.020820.69 0.1503.90.001480.557 0.6211774.90.008970.574 0.37777 1.40.000560.519 0.325756 2.80.015320.517 0.658584>10.00.024370.447 0.4051265.80.006880.602 0.6922089.50.008880.572 0.451010>10.00.078030.694 0.401404>10.00.017360.539 0.51319590.007720.464 0.7552050 3.80.097330.507 0.376165 3.30.006880.645 0.66712>10.00.00110.509 0.4782497.40.017690.585 0.3366606.60.036250.587 0.5871098.60.00970.678 0.3982017.80.010420.532 0.974265 5.60.011680.86 0.575120 3.70.005470.581 0.3184918.50.029120.67 0.328274>10.00.010820.469 0.26411090.003750.484 0.4863257.70.015030.544 0.237257 5.10.005680.505 0.3831811 5.30.077130.549 0.29290 6.70.012010.475 0.2563950.001450.383 0.448163 5.60.008040.74 0.4131267.20.004260.402 0.4064617.20.019290.3870.46126 5.50.006040.602 0.2781807.30.008730.496 0.15645>10.00.001770.415 0.2139480.004840.536 0.29100 5.90.003030.562 0.511319>10.00.034280.619 0.318371 5.40.010620.649 0.2145 3.70.005160.354 0.275204 6.70.007280.481 0.454476 4.80.011950.353 0.422161 4.80.006940.423 0.466146 6.60.004980.398。

CAS号413611-93-5_10074-G5_MedBio_物理性质

CAS
1、产品物理参数：
常用名
10074-G5
英文名
10074-G5
CAS号
413611-93-5
分子量
332.313
密度
1.4±0.1 g/cm3
沸点
538.6±60.0 °C at 760 mmHg
分子式
C18H12N4O3
熔点
无资料
闪点
279.5±32.9 °C
2、技术资料：
体外研究
10074-G5抑制Daudi Burkitt淋巴瘤细胞的生长并破坏c-Myc / Max二聚化。针对Daudi和HL-60细胞的IC50值分别为15.6和13.5μM[1]。10074-G5在区域Arg363-Ile381中结合Myc肽Myc353-437，Kd值为2.8μM。10074-G5结合在由诱导螺旋结构域（Leu370-Arg378）的N末端的扭结（Asp379-Ile381）产生的空腔中[3]。
≥98%
品牌
货号
中文名称
英文名称
CAS
包装
纯度
MedBio
MED11457
CDK inhibitor II
CDK inhibitor II
1269815-17-9
50mg
≥98%
品牌
货号
中文名称
英文名称
CAS
包装
纯度
MedBio
MED11591
(S)-CCG-1423
(S)-CCG-1423
None
体内研究
静脉注射20 mg / kg小鼠的血浆半衰期为10074-G5，为37分钟，血药浓度峰值为58μM，比肿瘤峰值浓度高10倍[1]。
3、同类产品列表：

JCR2015影响因子(所有期刊从高到低排序)+中科院分区

Rank ISSN Abbreviated JournalTitleFull Title Category2940007-9235CA-CANCER J CLIN CA-A CANCER JOURNAL FOR CLINICIAN医学10200028-4793NEW ENGL J MED NEW ENGLAND JOURNAL OF MEDICINE医学3410009-2665CHEM REV CHEMICAL REVIEWS化学17260140-6736LANCET LANCET医学63341474-1776NAT REV DRUG DISCOV NATURE REVIEWS DRUG DISCOVERY医学51741087-0156NAT BIOTECHNOL NATURE BIOTECHNOLOGY工程技术10020028-0836NATURE NATURE综合性期刊32570732-0582ANNU REV IMMUNOL ANNUAL REVIEW OF IMMUNOLOGY医学62471471-0072NAT REV MOL CELL BIO NATURE REVIEWS MOLECULAR CELL B生物63331474-175X NAT REV CANCER NATURE REVIEWS CANCER医学62461471-0056NAT REV GENET NATURE REVIEWS GENETICS生物63621476-1122NAT MATER NATURE MATERIALS工程技术16260098-7484JAMA-J AM MED ASSOC JAMA-JOURNAL OF THE AMERICAN ME医学63321474-1733NAT REV IMMUNOL NATURE REVIEWS IMMUNOLOGY医学75191748-3387NAT NANOTECHNOL Nature Nanotechnology工程技术12390036-8075SCIENCE SCIENCE综合性期刊27400306-0012CHEM SOC REV CHEMICAL SOCIETY REVIEWS化学14560066-4146ANNU REV ASTRON ASTR ANNUAL REVIEW OF ASTRONOMY AND地学天文75331749-4885NAT PHOTONICS Nature Photonics物理15680092-8674CELL CELL生物67681548-7091NAT METHODS NATURE METHODS生物62451471-003X NAT REV NEUROSCI Nature Reviews Neuroscience NEUROSCIENCES 14570066-4154ANNU REV BIOCHEM ANNUAL REVIEW OF BIOCHEMISTRY生物11940034-6861REV MOD PHYS REVIEWS OF MODERN PHYSICS物理48751061-4036NAT GENET NATURE GENETICS生物15060079-6425PROG MATER SCI PROGRESS IN MATERIALS SCIENCE工程技术51051078-8956NAT MED NATURE MEDICINE医学11140031-9333PHYSIOL REV PHYSIOLOGICAL REVIEWS医学15100079-6700PROG POLYM SCI PROGRESS IN POLYMER SCIENCE化学76261755-4330NAT CHEM Nature Chemistry化学62371470-2045LANCET ONCOL LANCET ONCOLOGY医学74001740-1526NAT REV MICROBIOL NATURE REVIEWS MICROBIOLOGY生物66151535-6108CANCER CELL CANCER CELL医学67091543-5008ANNU REV PLANT BIOL ANNUAL REVIEW OF PLANT BIOLOGY生物63191473-3099LANCET INFECT DIS LANCET INFECTIOUS DISEASES医学150001-4842ACCOUNTS CHEM RES ACCOUNTS OF CHEMICAL RESEARCH化学80331934-5909CELL STEM CELL Cell Stem Cell生物57941364-6613TRENDS COGN SCI TRENDS IN COGNITIVE SCIENCES医学63351474-4422LANCET NEUROL LANCET NEUROLOGY医学14660066-4308ANNU REV PSYCHOL ANNUAL REVIEW OF PSYCHOLOGY医学50621074-7613IMMUNITY IMMUNITY医学19000163-769X ENDOCR REV ENDOCRINE REVIEWS医学580001-8732ADV PHYS ADVANCES IN PHYSICS物理17250140-525X BEHAV BRAIN SCI BEHAVIORAL AND BRAIN SCIENCES医学76141754-5692ENERG ENVIRON SCI Energy & Environmental Science化学74791745-2473NAT PHYS Nature Physics物理29820370-1573PHYS REP PHYSICS REPORTS-REVIEW SECTION 物理65501529-2908NAT IMMUNOL NATURE IMMUNOLOGY医学61991465-7392NAT CELL BIOL NATURE CELL BIOLOGY生物84372159-8274CANCER DISCOV Cancer discovery ONCOLOGY 18180147-006X ANNU REV NEUROSCI ANNUAL REVIEW OF NEUROSCIENCE医学60511433-8351LIVING REV RELATIV Living Reviews in Relativity物理28850360-1285PROG ENERG COMBUST PROGRESS IN ENERGY AND COMBUST工程技术医学68241553-4006ANNU REV PATHOL-MECH Annual Review of Pathology-Mechanisms o14640066-4278ANNU REV PHYSIOL ANNUAL REVIEW OF PHYSIOLOGY医学32580732-183X J CLIN ONCOL JOURNAL OF CLINICAL ONCOLOGY医学29200362-1642ANNU REV PHARMACOL ANNUAL REVIEW OF PHARMACOLOGY 医学1520003-4819ANN INTERN MED ANNALS OF INTERNAL MEDICINE医学地学天文39930935-4956ASTRON ASTROPHYS REV ASTRONOMY AND ASTROPHYSICS REV71031614-4961LIVING REV SOL PHYS LIVING REVIEWS IN SOLAR PHYSICS ASTRONOMY & AS 67881550-4131CELL METAB Cell Metabolism生物39970935-9648ADV MATER ADVANCED MATERIALS工程技术76391756-1833BMJ-BRIT MED J BMJ-British Medical Journal MEDICINE, GENER 36650893-8512CLIN MICROBIOL REV CLINICAL MICROBIOLOGY REVIEWS医学1830003-9926ARCH INTERN MED ARCHIVES OF INTERNAL MEDICINE医学10940031-6997PHARMACOL REV PHARMACOLOGICAL REVIEWS医学680002-5100ALDRICHIM ACTA ALDRICHIMICA ACTA化学11890034-4885REP PROG PHYS REPORTS ON PROGRESS IN PHYSICS物理生物26600301-5556ADV ANAT EMBRYOL CEL ADVANCES IN ANATOMY EMBRYOLOGY化学14630066-426X ANNU REV PHYS CHEM ANNUAL REVIEW OF PHYSICAL CHEMIS5210016-5085GASTROENTEROLOGY GASTROENTEROLOGY医学51251081-0706ANNU REV CELL DEV BI ANNUAL REVIEW OF CELL AND DEVELO生物医学32960735-1097J AM COLL CARDIOL JOURNAL OF THE AMERICAN COLLEGE20440169-5347TRENDS ECOL EVOL TRENDS IN ECOLOGY & EVOLUTION生物71041614-6832ADV ENERGY MATER Advanced Energy Materials CHEMISTRY, PHYS 52791097-6256NAT NEUROSCI NATURE NEUROSCIENCE医学化学59481389-5567J PHOTOCH PHOTOBIO C JOURNAL OF PHOTOCHEMISTRY AND P81491946-6234SCI TRANSL MED Science Translational Medicine CELL BIOLOGY医学64501520-765X EUR HEART J SUPPL EUROPEAN HEART JOURNAL SUPPLEM14600066-4197ANNU REV GENET ANNUAL REVIEW OF GENETICS生物39160927-796X MAT SCI ENG R MATERIALS SCIENCE & ENGINEERING 工程技术80491936-122X ANNU REV BIOPHYS Annual Review of Biophysics生物76891759-4758NAT REV NEUROL Nature Reviews Neurology医学21640195-668X EUR HEART J EUROPEAN HEART JOURNAL医学37130896-6273NEURON NEURON医学20380169-409X ADV DRUG DELIVER REV ADVANCED DRUG DELIVERY REVIEWS医学75131748-0132NANO TODAY Nano Today工程技术86088755-1209REV GEOPHYS REVIEWS OF GEOPHYSICS地学81551947-5454ANNU REV CONDEN MA P Annual Review of Condensed Matter Physi PHYSICS, CONDEN 19620167-5729SURF SCI REP SURFACE SCIENCE REPORTS化学11550033-2909PSYCHOL BULL PSYCHOLOGICAL BULLETIN医学5530017-5749GUT GUT医学51931088-9051GENOME RES GENOME RESEARCH生物生物52311092-2172MICROBIOL MOL BIOL R MICROBIOLOGY AND MOLECULAR BIOL83072047-7538LIGHT-SCI APPL Light-Science & Applications OPTICS 76811758-678X NAT CLIM CHANGE Nature climate change ENVIRONMENTAL 57581359-4184MOL PSYCHIATR MOLECULAR PSYCHIATRY医学1820003-990X ARCH GEN PSYCHIAT ARCHIVES OF GENERAL PSYCHIATRY医学3590009-7322CIRCULATION CIRCULATION医学67721549-1676PLOS MED PLOS MEDICINE MEDICINE, GENER 49041063-5157SYST BIOL SYSTEMATIC BIOLOGY生物81041941-1405ANNU REV MAR SCI Annual Review of Marine Science地学73441723-8617WORLD PSYCHIATRY World Psychiatry医学64881523-9829ANNU REV BIOMED ENG ANNUAL REVIEW OF BIOMEDICAL ENG工程技术76901759-4774NAT REV CLIN ONCOL Nature Reviews Clinical Oncology医学58401369-7021MATER TODAY Materials Today工程技术52771097-2765MOL CELL MOLECULAR CELL生物26720302-2838EUR UROL EUROPEAN UROLOGY医学14580066-4170ANNU REV ENTOMOL ANNUAL REVIEW OF ENTOMOLOGY生物65641530-6984NANO LETT NANO LETTERS工程技术19380166-2236TRENDS NEUROSCI TRENDS IN NEUROSCIENCES医学67381545-9993NAT STRUCT MOL BIOL NATURE STRUCTURAL & MOLECULAR BBIOCHEMISTRY & 76941759-5029NAT REV ENDOCRINOL Nature Reviews Endocrinology医学19350166-0616STUD MYCOL STUDIES IN MYCOLOGY生物20150168-6445FEMS MICROBIOL REV FEMS MICROBIOLOGY REVIEWS生物医学7260021-9738J CLIN INVEST JOURNAL OF CLINICAL INVESTIGATION84752168-6106JAMA INTERN MED JAMA Internal Medicine MEDICINE, GENER医学50501073-449X AM J RESP CRIT CARE AMERICAN JOURNAL OF RESPIRATORY68091552-4450NAT CHEM BIOL Nature Chemical Biology生物57731360-1385TRENDS PLANT SCI TRENDS IN PLANT SCIENCE生物14610066-4219ANNU REV MED ANNUAL REVIEW OF MEDICINE医学80481936-0851ACS NANO ACS Nano工程技术医学67671548-5943ANNU REV CLIN PSYCHO ANNUAL REVIEW OF CLINICAL PSYCHO76951759-5045NAT REV GASTRO HEPAT Nature Reviews Gastroenterology & Hepat医学10000027-8874JNCI-J NATL CANCER I JNCI-Journal of the National Cancer Institu ONCOLOGY医学7570022-1007J EXP MED JOURNAL OF EXPERIMENTAL MEDICINE44551001-0602CELL RES CELL RESEARCH生物68181552-5260ALZHEIMERS DEMENT Alzheimers & Dementia医学79881931-3128CELL HOST MICROBE Cell Host & Microbe生物1050002-953X AM J PSYCHIAT AMERICAN JOURNAL OF PSYCHIATRY医学3970010-8545COORDIN CHEM REV COORDINATION CHEMISTRY REVIEWS化学14620066-4227ANNU REV MICROBIOL ANNUAL REVIEW OF MICROBIOLOGY生物770002-7863J AM CHEM SOC JOURNAL OF THE AMERICAN CHEMICA化学84802168-622X JAMA PSYCHIAT JAMA Psychiatry PSYCHIATRY 43000962-8924TRENDS CELL BIOL TRENDS IN CELL BIOLOGY生物19870167-7799TRENDS BIOTECHNOL TRENDS IN BIOTECHNOLOGY工程技术76871759-0876WIRES COMPUT MOL SCI W IREs Computational Molecular Science CHEMISTRY, MUL 65801531-7331ANNU REV MATER RES ANNUAL REVIEW OF MATERIALS RESE工程技术1150003-0007B AM METEOROL SOC BULLETIN OF THE AMERICAN METEOR地学71151616-301X ADV FUNCT MATER ADVANCED FUNCTIONAL MATERIALS工程技术68331554-8627AUTOPHAGY Autophagy生物75871752-0894NAT GEOSCI Nature Geoscience地学医学19310165-6147TRENDS PHARMACOL SCI TRENDS IN PHARMACOLOGICAL SCIEN15560091-6749J ALLERGY CLIN IMMUN JOURNAL OF ALLERGY AND CLINICAL I医学82642041-1723NAT COMMUN Nature communications Biological Science Disciplines 20200168-8278J HEPATOL JOURNAL OF HEPATOLOGY医学化学60491433-7851ANGEW CHEM INT EDIT ANGEWANDTE CHEMIE-INTERNATIONA19030163-8998ANNU REV NUCL PART S ANNUAL REVIEW OF NUCLEAR AND PA物理43630968-0004TRENDS BIOCHEM SCI TRENDS IN BIOCHEMICAL SCIENCES生物14690067-0049ASTROPHYS J SUPPL S ASTROPHYSICAL JOURNAL SUPPLEME地学天文物理14590066-4189ANNU REV FLUID MECH ANNUAL REVIEW OF FLUID MECHANICS24880270-9139HEPATOLOGY HEPATOLOGY医学3600009-7330CIRC RES CIRCULATION RESEARCH医学940002-9297AM J HUM GENET AMERICAN JOURNAL OF HUMAN GENE生物74601744-4292MOL SYST BIOL Molecular Systems Biology生物61981465-6906GENOME BIOL GENOME BIOLOGY BIOTECHNOLOGY 36240890-9369GENE DEV GENES & DEVELOPMENT生物370001-6322ACTA NEUROPATHOL ACTA NEUROPATHOLOGICA医学930002-9270AM J GASTROENTEROL AMERICAN JOURNAL OF GASTROENTE医学61551461-023X ECOL LETT ECOLOGY LETTERS环境科学67111543-592X ANNU REV ECOL EVOL S ANNUAL REVIEW OF ECOLOGY EVOLU生物2530006-4971BLOOD BLOOD医学84252157-1724KIDNEY INT SUPPL Kidney International Supplements UROLOGY & NEPHROLOGY 23760261-4189EMBO J EMBO JOURNAL生物35670887-6924LEUKEMIA LEUKEMIA医学62881471-4906TRENDS IMMUNOL TRENDS IN IMMUNOLOGY医学医学1580003-4967ANN RHEUM DIS ANNALS OF THE RHEUMATIC DISEASES85252211-2855NANO ENERGY Nano Energy CHEMISTRY, PHYS 2400006-3223BIOL PSYCHIAT BIOLOGICAL PSYCHIATRY医学16200098-2997MOL ASPECTS MED MOLECULAR ASPECTS OF MEDICINE医学57341355-4786HUM REPROD UPDATE HUMAN REPRODUCTION UPDATE医学16720105-2896IMMUNOL REV IMMUNOLOGICAL REVIEWS医学79641884-4049NPG ASIA MATER NPG Asia Materials MATERIALS SCIEN 81361943-8206ADV OPT PHOTONICS Advances in Optics and Photonics OPTICS24090265-0568NAT PROD REP NATURAL PRODUCT REPORTS化学85472214-109X LANCET GLOB HEALTH Lancet Global Health PUBLIC, ENVIRON 19010163-7827PROG LIPID RES PROGRESS IN LIPID RESEARCH生物28830360-0564ADV CATAL ADVANCES IN CATALYSIS化学26360301-0082PROG NEUROBIOL PROGRESS IN 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医学47361046-6673J AM SOC NEPHROL JOURNAL OF THE AMERICAN SOCIETY 医学67321545-7885PLOS BIOL PLoS Biology BIOCHEMISTRY &46501040-4651PLANT CELL PLANT CELL 生物47171044-579X SEMIN CANCER BIOL SEMINARS IN CANCER BIOLOGY 医学3220008-5472CANCER RES CANCER RESEARCH 医学83162050-084X ELIFE eLife BIOLOGY 84112155-5435ACS CATAL ACS catalysis CHEMISTRY, PHYSICAL 75601751-7362ISME J ISME Journal 环境科学82692041-6520CHEM SCI Chemical science CHEMISTRY, MULT 2610006-8950BRAIN BRAIN 医学11620033-3190PSYCHOTHER PSYCHOSOM PSYCHOTHERAPY AND PSYCHOSOMAT 医学43460966-842X TRENDS MICROBIOL TRENDS IN MICROBIOLOGY 生物85462213-8587LANCET DIABETES ENDO Lancet Diabetes & Endocrinology ENDOCRINOLOGY 76931759-5002NAT REV CARDIOL Nature Reviews Cardiology 医学26130300-5771INT J EPIDEMIOL INTERNATIONAL JOURNAL OF EPIDEMI 医学58301368-7646DRUG RESIST UPDATE DRUG RESISTANCE UPDATES 医学27240305-1048NUCLEIC ACIDS RES NUCLEIC ACIDS RESEARCH 生物33250737-4038MOL BIOL EVOL MOLECULAR BIOLOGY AND EVOLUTION 生物62311469-221X EMBO REP EMBO REPORTS 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BIOCHEMISTRY & 26820303-4240REV PHYSIOL BIOCH P REVIEWS OF PHYSIOLOGY BIOCHEMIS医学84482162-4011ONCOIMMUNOLOGY OncoImmunology ONCOLOGY生物51631084-9521SEMIN CELL DEV BIOL SEMINARS IN CELL & DEVELOPMENTAL9450025-6196MAYO CLIN PROC MAYO CLINIC PROCEEDINGS医学工程技术57541359-0286CURR OPIN SOLID ST M CURRENT OPINION IN SOLID STATE & M79001873-9946J CROHNS COLITIS Journal of Crohns & Colitis医学64971525-0016MOL THER MOLECULAR THERAPY医学14460065-308X ADV PARASIT ADVANCES IN PARASITOLOGY医学68991560-2745FUNGAL DIVERS FUNGAL DIVERSITY生物51441083-4419IEEE T SYST MAN CY B IEEE TRANSACTIONS ON SYSTEMS MA工程技术81121941-7640CIRC-CARDIOVASC INTE Circulation: Cardiovascular Interventions CARDIAC & CARDI 30630378-7753J POWER SOURCES JOURNAL OF POWER SOURCES工程技术生物33830748-3007CLADISTICS CLADISTICS-THE INTERNATIONAL JOUR生物58771383-5742MUTAT RES-REV MUTAT MUTATION RESEARCH-REVIEWS IN MU7250021-972X J CLIN ENDOCR METAB JOURNAL OF CLINICAL ENDOCRINOLO医学62901471-4922TRENDS PARASITOL TRENDS IN 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CRITICAL REVIEWS IN MICROBIOLOGY生物76631757-7004WIRES RNA WIRES RNA CELL BIOLOGY工程技术35420885-8993IEEE T POWER ELECTR IEEE TRANSACTIONS ON POWER ELEC51121079-5642ARTERIOSCL THROM VAS ARTERIOSCLEROSIS THROMBOSIS AN医学7830022-1899J INFECT DIS JOURNAL OF INFECTIOUS DISEASES医学2090004-637X ASTROPHYS J ASTROPHYSICAL JOURNAL地学天文28390340-5761ARCH TOXICOL ARCHIVES OF TOXICOLOGY医学42700960-7412PLANT J PLANT JOURNAL生物49631065-9471HUM BRAIN MAPP HUMAN BRAIN MAPPING医学5280016-6731GENETICS GENETICS生物14410065-2776ADV IMMUNOL ADVANCES IN IMMUNOLOGY医学34470820-3946CAN MED ASSOC J CANADIAN MEDICAL ASSOCIATION JOU医学3260008-6363CARDIOVASC RES CARDIOVASCULAR RESEARCH医学11560033-2917PSYCHOL MED PSYCHOLOGICAL MEDICINE医学43190964-4563TOB CONTROL TOBACCO CONTROL医学57881364-0321RENEW SUST ENERG REV R ENEWABLE & SUSTAINABLE ENERGY工程技术医学25140272-6386AM J KIDNEY DIS AMERICAN JOURNAL OF KIDNEY DISEA58371369-5274CURR OPIN MICROBIOL CURRENT OPINION IN MICROBIOLOGY生物环境科学67121543-5938ANNU REV ENV RESOUR ANNUAL REVIEW OF ENVIRONMENT AN81081941-3289CIRC-HEART FAIL Circulation-Heart Failure医学35880889-1591BRAIN BEHAV IMMUN BRAIN BEHAVIOR AND IMMUNITY医学1350003-3022ANESTHESIOLOGY ANESTHESIOLOGY医学47501049-250X ADV ATOM MOL OPT PHY ADVANCES IN ATOMIC MOLECULAR AN物理医学53951180-4882J PSYCHIATR NEUROSCI JOURNAL OF PSYCHIATRY & NEUROSC47961053-5888IEEE SIGNAL PROC MAG IEEE SIGNAL PROCESSING MAGAZINE工程技术57551359-0294CURR OPIN COLLOID IN CURRENT OPINION IN COLLOID & INTE化学31020390-6078HAEMATOLOGICA HAEMATOLOGICA HEMATOLOGY地学天文63551475-7516J COSMOL ASTROPART P JOURNAL OF COSMOLOGY AND ASTRO生物60021420-682X CELL MOL LIFE SCI CMLS-Cellular and Molecular Life Sciences9170024-9297MACROMOLECULES MACROMOLECULES工程技术84962192-2640ADV HEALTHC MATER Advanced healthcare materials工程技术82952046-2441OPEN BIOL Open biology BIOCHEMISTRY &工程技术18870162-8828IEEE T PATTERN ANAL IEEE TRANSACTIONS ON PATTERN ANA54041198-743X CLIN MICROBIOL INFEC CLINICAL MICROBIOLOGY AND INFECT医学84402161-1653ACS MACRO LETT ACS Macro Letters化学62151467-7644PLANT BIOTECHNOL J PLANT BIOTECHNOLOGY JOURNAL生物62121467-3037CURR ISSUES MOL BIOL CURRENT ISSUES IN MOLECULAR BIOL生物65071525-7797BIOMACROMOLECULES BIOMACROMOLECULES化学36340891-5849FREE RADICAL BIO MED FREE RADICAL BIOLOGY AND MEDICIN医学40690947-6539CHEM-EUR J CHEMISTRY-A EUROPEAN JOURNAL化学50391072-4710ARCH PEDIAT ADOL MED ARCHIVES OF PEDIATRICS & ADOLESC医学医学24640269-2813ALIMENT PHARM THER ALIMENTARY PHARMACOLOGY & THER9590025-7974MEDICINE MEDICINE医学12840039-2499STROKE STROKE医学69011560-7917EUROSURVEILLANCE EUROSURVEILLANCE INFECTIOUS DISEASES 66541538-7933J THROMB HAEMOST JOURNAL OF THROMBOSIS AND HAEM医学84262157-6564STEM CELL TRANSL MED Stem cells translational medicine CELL & TISSUE ENGINEERING 15130079-6816PROG SURF SCI PROGRESS IN SURFACE SCIENCE工程技术84982192-8606NANOPHOTONICS-BERLIN N anophotonics NANOSCIENCE &66161535-7163MOL CANCER THER MOLECULAR CANCER THERAPEUTICS医学医学70591600-6135AM J TRANSPLANT AMERICAN JOURNAL OF TRANSPLANTA35210885-3185MOVEMENT DISORD MOVEMENT DISORDERS医学35010883-7694MRS BULL MRS BULLETIN工程技术26690301-9268PRECAMBRIAN RES PRECAMBRIAN RESEARCH地学71071615-4150ADV SYNTH CATAL ADVANCED SYNTHESIS & CATALYSIS化学42180957-9672CURR OPIN LIPIDOL CURRENT OPINION IN LIPIDOLOGY医学81131941-7705CIRC-CARDIOVASC QUAL Circulation: Cardiovascular Quality and Ou CARDIAC & CARDI1320003-2700ANAL CHEM ANALYTICAL CHEMISTRY化学26970304-3835CANCER LETT CANCER LETTERS医学84312158-3188TRANSL PSYCHIAT Translational Psychiatry PSYCHIATRY43740969-2126STRUCTURE STRUCTURE生物78331863-2653BRAIN STRUCT FUNCT Brain Structure & Function医学27410306-2619APPL ENERG APPLIED ENERGY工程技术450001-690X ACTA PSYCHIAT SCAND ACTA PSYCHIATRICA SCANDINAVICA医学17750143-5221CLIN SCI CLINICAL SCIENCE医学57371355-6037HEART HEART医学82852045-2322SCI REP-UK Scientific reports Natural Science Disciplines 47861052-9276REV MED VIROL REVIEWS IN MEDICAL VIROLOGY医学34040749-6419INT J PLASTICITY INTERNATIONAL JOURNAL OF PLASTIC工程技术24600268-960X BLOOD REV BLOOD REVIEWS医学64761522-8517NEURO-ONCOLOGY NEURO-ONCOLOGY医学18600160-4120ENVIRON INT ENVIRONMENT INTERNATIONAL环境科学24800269-9370AIDS AIDS医学2780007-1323BRIT J SURG BRITISH JOURNAL OF SURGERY医学13480043-1354WATER RES WATER RESEARCH环境科学57241354-3784EXPERT OPIN INV DRUG EXPERT OPINION ON INVESTIGATIONA医学7550022-0957J EXP BOT JOURNAL OF EXPERIMENTAL BOTANY生物7450022-0477J ECOL JOURNAL OF ECOLOGY环境科学77031759-9954POLYM CHEM-UK POLYMER CHEMISTRY POLYMER SCIENCE 82141994-0416CRYOSPHERE Cryosphere GEOGRAPHY, PHY 18630160-6689J CLIN PSYCHIAT JOURNAL OF CLINICAL PSYCHIATRY医学67821549-9618J CHEM THEORY COMPUT J ournal of Chemical Theory and Computat化学65401527-8999CHEM REC CHEMICAL RECORD化学61951465-542X BREAST CANCER RES BREAST CANCER RESEARCH ONCOLOGY。

2012年公布最新JCR分区表(sci分区,影响因子,引频次)

刊名简称刊名全称ISSN PLOS PATHOG PLoS Pathogens1553-7366 REV MED VIROL REVIEWS IN MEDICAL VIROLOGY1052-9276 ADV VIRUS RES ADVANCES IN VIRUS RESEARCH0065-3527 AIDS AIDS0269-9370 ANTIVIR THER ANTIVIRAL THERAPY1359-6535 J VIROL JOURNAL OF VIROLOGY0022-538X RETROVIROLOGY Retrovirology 1742-4690 ANTIVIR RES ANTIVIRAL RESEARCH0166-3542 INFLUENZA OTHER RESP Influenza and Other Respiratory Viruses1750-2640 INT J MED MICROBIOL INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY1438-4221 J CLIN VIROL JOURNAL OF CLINICAL VIROLOGY1386-6532 J GEN VIROL JOURNAL OF GENERAL VIROLOGY0022-1317 J MED VIROL JOURNAL OF MEDICAL VIROLOGY0146-6615 J VIRAL HEPATITIS JOURNAL OF VIRAL HEPATITIS1352-0504 MICROBES INFECT MICROBES AND INFECTION1286-4579 VIROLOGY VIROLOGY0042-6822 ACTA VIROL ACTA VIROLOGICA0001-723X AIDS RES HUM RETROV AIDS RESEARCH AND HUMAN RETROVIRUSES0889-2229 ARCH VIROL ARCHIVES OF VIROLOGY0304-8608 CURR HIV RES CURRENT HIV RESEARCH1570-162X FOOD ENVIRON VIROL Food and Environmental Virology1867-0334 FUTURE VIROL Future Virology1746-0794 INDIAN J VIROL Indian Journal of Virology 0970-2822 INTERVIROLOGY INTERVIROLOGY0300-5526 J NEUROVIROL JOURNAL OF NEUROVIROLOGY1355-0284 J VIROL METHODS JOURNAL OF VIROLOGICAL METHODS0166-0934 S AFR J HIV MED SOUTHERN AFRICAN JOURNAL OF HIV MEDICINE1608-9693 VIRAL IMMUNOL VIRAL IMMUNOLOGY0882-8245 VIROL J Virology Journal1743-422X VIROLOGIE VIROLOGIE1267-8694 VIRUS GENES VIRUS GENES0920-8569 VIRUS RES VIRUS RESEARCH0168-1702 VIRUSES-BASEL Viruses-Basel1999-4915 ACTA NEUROPATHOL ACTA NEUROPATHOLOGICA0001-6322 AM J PATHOL AMERICAN JOURNAL OF PATHOLOGY0002-9440 ANNU REV PATHOL-MECH Annual Review of Pathology-Mechanisms of Disease1553-4006 J PATHOL JOURNAL OF PATHOLOGY0022-3417 AM J SURG PATHOL AMERICAN JOURNAL OF SURGICAL PATHOLOGY0147-5185 BRAIN PATHOL BRAIN PATHOLOGY1015-6305 DIS MODEL MECH Disease Models & Mechanisms1754-8403 EXPERT REV MOL DIAGN EXPERT REVIEW OF MOLECULAR DIAGNOSTICS1473-7159 HISTOPATHOLOGY HISTOPATHOLOGY0309-0167 J MOL DIAGN JOURNAL OF MOLECULAR DIAGNOSTICS1525-1578 J NEUROPATH EXP NEUR JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEURO0022-3069 LAB INVEST LABORATORY INVESTIGATION0023-6837 MODERN PATHOL MODERN PATHOLOGY0893-3952 NEUROPATH APPL NEURO NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY0305-1846 SEMIN IMMUNOPATHOL Seminars in Immunopathology1863-2297 ADV ANAT PATHOL ADVANCES IN ANATOMIC PATHOLOGY1072-4109 ALZ DIS ASSOC DIS ALZHEIMER DISEASE & ASSOCIATED DISORDERS0893-0341 AM J CLIN PATHOL AMERICAN JOURNAL OF CLINICAL PATHOLOGY0002-9173ANAL CELL PATHOL Analytical Cellular Pathology-Cellular Oncology2210-7177 ARCH PATHOL LAB MED ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE0003-9985 CANCER CYTOPATHOL CANCER CYTOPATHOLOGY1934-662X CYTOM PART B-CLIN CY CYTOMETRY PART B-CLINICAL CYTOMETRY1552-4949 DIS MARKERS DISEASE MARKERS0278-0240 EXP MOL PATHOL EXPERIMENTAL AND MOLECULAR PATHOLOGY0014-4800 HISTOL HISTOPATHOL HISTOLOGY AND HISTOPATHOLOGY0213-3911 HUM PATHOL HUMAN PATHOLOGY0046-8177 INT J EXP PATHOL INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY0959-9673 INT J IMMUNOPATH PH INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHA0394-6320 J CLIN PATHOL JOURNAL OF CLINICAL PATHOLOGY0021-9746 PATHOBIOLOGY PATHOBIOLOGY1015-2008 PATHOLOGY PATHOLOGY0031-3025 TISSUE ANTIGENS TISSUE ANTIGENS0001-2815 TOXICOL PATHOL TOXICOLOGIC PATHOLOGY0192-6233 VIRCHOWS ARCH VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATH0945-6317 ACTA CYTOL ACTA CYTOLOGICA0001-5547 AM J FOREN MED PATH AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOL0195-7910 ANN DIAGN PATHOL Annals of Diagnostic Pathology 1092-9134 ANN PATHOL ANNALES DE PATHOLOGIE0242-6498 APMIS APMIS0903-4641 APPL IMMUNOHISTO M M APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOL1062-3345 BRAIN TUMOR PATHOL Brain Tumor Pathology1433-7398 CARDIOVASC PATHOL CARDIOVASCULAR PATHOLOGY1054-8807 CLIN NEUROPATHOL CLINICAL NEUROPATHOLOGY0722-5091 CYTOPATHOLOGY CYTOPATHOLOGY0956-5507 DIAGN CYTOPATHOL DIAGNOSTIC CYTOPATHOLOGY8755-1039 DIAGN MOL PATHOL DIAGNOSTIC MOLECULAR PATHOLOGY1052-9551 DIAGN PATHOL Diagnostic Pathology1746-1596 ENDOCR PATHOL ENDOCRINE PATHOLOGY1046-3976 EXP TOXICOL PATHOL EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY0940-2993 FETAL PEDIATR PATHOL Fetal and Pediatric Pathology1551-3815 FOLIA NEUROPATHOL FOLIA NEUROPATHOLOGICA1641-4640 FORENSIC SCI MED PAT Forensic Science Medicine and Pathology1547-769X INDIAN J PATHOL MICR Indian Journal of Pathology and Microbiology0377-4929 INT J GYNECOL PATHOL INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY0277-1691 INT J SURG PATHOL INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY1066-8969 J COMP PATHOL JOURNAL OF COMPARATIVE PATHOLOGY0021-9975 J CUTAN PATHOL JOURNAL OF CUTANEOUS PATHOLOGY0303-6987 J ORAL PATHOL MED JOURNAL OF ORAL PATHOLOGY & MEDICINE0904-2512 J TOXICOL PATHOL Journal of Toxicologic Pathology0914-9198 KOREAN J PATHOL Korean Journal of Pathology1738-1843 LEPROSY REV LEPROSY REVIEW0305-7518 MED MOL MORPHOL Medical Molecular Morphology1860-1480 MED NUCL Medecine Nucleaire-Imagerie Fonctionnelle et Met0928-1258 NEUROPATHOLOGY NEUROPATHOLOGY0919-6544 PATHOL BIOL PATHOLOGIE BIOLOGIE0369-8114 PATHOL INT PATHOLOGY INTERNATIONAL1320-5463 PATHOL ONCOL RES PATHOLOGY & ONCOLOGY RESEARCH1219-4956 PATHOL RES PRACT PATHOLOGY RESEARCH AND PRACTICE0344-0338 PATHOLOGE PATHOLOGE0172-8113 PEDIATR DEVEL PATHOL PEDIATRIC AND DEVELOPMENTAL PATHOLOGY1093-5266POL J PATHOL POLISH JOURNAL OF PATHOLOGY1233-9687 SCI JUSTICE SCIENCE & JUSTICE1355-0306 SEMIN DIAGN PATHOL SEMINARS IN DIAGNOSTIC PATHOLOGY0740-2570 ULTRASTRUCT PATHOL ULTRASTRUCTURAL PATHOLOGY0191-3123 VET PATHOL VETERINARY PATHOLOGY0300-9858 POLYM TEST POLYMER TESTING0142-9418 PROG CRYST GROWTH CH PROGRESS IN CRYSTAL GROWTH AND CHARACTERIZATION 0960-8974 EXP MECH EXPERIMENTAL MECHANICS0014-4851 MATER CHARACT MATERIALS CHARACTERIZATION1044-5803 NANOSC MICROSC THERM Nanoscale and Microscale Thermophysical Engineer1556-7265 NDT&E INT NDT & E INTERNATIONAL0963-8695 ENG FAIL ANAL ENGINEERING FAILURE ANALYSIS1350-6307 J NONDESTRUCT EVAL JOURNAL OF NONDESTRUCTIVE EVALUATION0195-9298 J SANDW STRUCT MATER JOURNAL OF SANDWICH STRUCTURES & MATERIALS1099-6362 J STRAIN ANAL ENG JOURNAL OF STRAIN ANALYSIS FOR ENGINEERING DESIG0309-3247 MECH ADV MATER STRUC MECHANICS OF ADVANCED MATERIALS AND STRUCTURES1537-6494 MECH TIME-DEPEND MAT MECHANICS OF TIME-DEPENDENT MATERIALS1385-2000 RES NONDESTRUCT EVAL RESEARCH IN NONDESTRUCTIVE EVALUATION0934-9847 STRAIN STRAIN0039-2103 ADV STEEL CONSTR Advanced Steel Construction1816-112X ARCH MECH ARCHIVES OF MECHANICS0373-2029 BETON- STAHLBETONBAU Beton- und Stahlbetonbau0005-9900 COMPUT CONCRETE Computers and Concrete 1598-8198 EXP TECHNIQUES EXPERIMENTAL TECHNIQUES0732-8818 INSIGHT INSIGHT1354-2575 J TEST EVAL JOURNAL OF TESTING AND EVALUATION0090-3973 MATER EVAL MATERIALS EVALUATION0025-5327 MATER PERFORMANCE MATERIALS PERFORMANCE0094-1492 MATER TEST Materials Testing-Materials and Components Techn0025-5300 NONDESTRUCT TEST EVA Nondestructive Testing and Evaluation1058-9759 PART PART SYST CHAR PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION0934-0866 POLYM POLYM COMPOS POLYMERS & POLYMER COMPOSITES0967-3911 POWDER DIFFR POWDER DIFFRACTION0885-7156 QIRT J QIRT Journal1768-6733 RUSS J NONDESTRUCT+RUSSIAN JOURNAL OF NONDESTRUCTIVE TESTING1061-8309 STRENGTH MATER+STRENGTH OF MATERIALS0039-2316 DYES PIGMENTS DYES AND PIGMENTS0143-7208 CELLULOSE CELLULOSE0969-0239 COLOR TECHNOL COLORATION TECHNOLOGY1472-3581 TEXT RES J TEXTILE RESEARCH JOURNAL0040-5175 FIBER POLYM FIBERS AND POLYMERS1229-9197 IND TEXTILA Industria Textila 1222-5347 J AM LEATHER CHEM AS JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIA0002-9726 J ENG FIBER FABR Journal of Engineered Fibers and Fabrics 1558-9250 J IND TEXT Journal of Industrial Textiles1528-0837 WOOD FIBER SCI WOOD AND FIBER SCIENCE0735-6161 AATCC REV AATCC REVIEW1532-8813 FIBRE CHEM+FIBRE CHEMISTRY0015-0541 FIBRES TEXT EAST EUR FIBRES & TEXTILES IN EASTERN EUROPE1230-3666 INT J CLOTH SCI TECH International Journal of Clothing Science and Te0955-6222 J NAT FIBERS Journal of Natural Fibers1544-0478 J SOC LEATH TECH CH JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS 0144-0322J TEXT I JOURNAL OF THE TEXTILE INSTITUTE0040-5000 J VINYL ADDIT TECHN JOURNAL OF VINYL & ADDITIVE TECHNOLOGY1083-5601 SEN-I GAKKAISHI SEN-I GAKKAISHI0037-9875 TEKST KONFEKSIYON Tekstil ve Konfeksiyon 1300-3356 TEKSTIL TEKSTIL0492-5882 COMPOS SCI TECHNOL COMPOSITES SCIENCE AND TECHNOLOGY0266-3538 COMPOS PART A-APPL S COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTUR1359-835X COMPOS PART B-ENG COMPOSITES PART B-ENGINEERING1359-8368 COMPOS STRUCT COMPOSITE STRUCTURES0263-8223 APPL COMPOS MATER APPLIED COMPOSITE MATERIALS0929-189X CEMENT CONCRETE COMP CEMENT & CONCRETE COMPOSITES0958-9465 J COMPOS CONSTR JOURNAL OF COMPOSITES FOR CONSTRUCTION1090-0268 J COMPOS MATER JOURNAL OF COMPOSITE MATERIALS0021-9983 J SANDW STRUCT MATER JOURNAL OF SANDWICH STRUCTURES & MATERIALS1099-6362 MECH ADV MATER STRUC MECHANICS OF ADVANCED MATERIALS AND STRUCTURES1537-6494 POLYM COMPOSITE POLYMER COMPOSITES0272-8397 ADV COMPOS LETT ADVANCED COMPOSITES LETTERS0963-6935 ADV COMPOS MATER ADVANCED COMPOSITE MATERIALS0924-3046 BETON- STAHLBETONBAU Beton- und Stahlbetonbau0005-9900 CEM WAPNO BETON Cement Wapno Beton1425-8129 COMPOS INTERFACE COMPOSITE INTERFACES0927-6440 J REINF PLAST COMP JOURNAL OF REINFORCED PLASTICS AND COMPOSITES0731-6844 J THERMOPLAST COMPOS JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS0892-7057 MECH COMPOS MATER MECHANICS OF COMPOSITE MATERIALS0191-5665 PLAST RUBBER COMPOS PLASTICS RUBBER AND COMPOSITES1465-8011 POLYM POLYM COMPOS POLYMERS & POLYMER COMPOSITES0967-3911 PROG RUBBER PLAST RE Progress in Rubber Plastics and Recycling Techno1477-7606 SCI ENG COMPOS MATER SCIENCE AND ENGINEERING OF COMPOSITE MATERIALS0334-181X STEEL COMPOS STRUCT STEEL AND COMPOSITE STRUCTURES1229-9367 J EUR CERAM SOC JOURNAL OF THE EUROPEAN CERAMIC SOCIETY0955-2219 CERAM INT CERAMICS INTERNATIONAL0272-8842 INT J APPL CERAM TEC International Journal of Applied Ceramic Technol1546-542X J AM CERAM SOC JOURNAL OF THE AMERICAN CERAMIC SOCIETY0002-7820 ADV APPL CERAM Advances in Applied Ceramics1743-6753 J CERAM SOC JPN JOURNAL OF THE CERAMIC SOCIETY OF JAPAN1882-0743 J ELECTROCERAM JOURNAL OF ELECTROCERAMICS1385-3449 J NON-CRYST SOLIDS JOURNAL OF NON-CRYSTALLINE SOLIDS0022-3093 J SOL-GEL SCI TECHN JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY0928-0707 AM CERAM SOC BULL AMERICAN CERAMIC SOCIETY BULLETIN0002-7812 BOL SOC ESP CERAM V BOLETIN DE LA SOCIEDAD ESPANOLA DE CERAMICA Y VI0366-3175 CERAM-SILIKATY CERAMICS-SILIKATY0862-5468 CFI-CERAM FORUM INT CFI-CERAMIC FORUM INTERNATIONAL0173-9913 GLASS CERAM+GLASS AND CERAMICS0361-7610 GLASS PHYS CHEM+GLASS PHYSICS AND CHEMISTRY1087-6596 GLASS TECHNOL-PART A GLASS TECHNOLOGY1753-3546 IND CERAM INDUSTRIAL CERAMICS1121-7588 J CERAM PROCESS RES JOURNAL OF CERAMIC PROCESSING RESEARCH1229-9162 J INORG MATER JOURNAL OF INORGANIC MATERIALS1000-324X MATER WORLD MATERIALS WORLD0967-8638 PHYS CHEM GLASSES-B Physics and Chemistry of Glasses-European Journa1753-3562 POWDER METALL MET C+POWDER METALLURGY AND METAL CERAMICS1068-1302 REFRACT IND CERAM+REFRACTORIES AND INDUSTRIAL CERAMICS1083-4877SCI SINTER SCIENCE OF SINTERING0350-820X T INDIAN CERAM SOC Transactions of the Indian Ceramic Society 0371-750X J ELECTROCHEM SOC JOURNAL OF THE ELECTROCHEMICAL SOCIETY0013-4651 CHEM VAPOR DEPOS CHEMICAL VAPOR DEPOSITION0948-1907 SURF COAT TECH SURFACE & COATINGS TECHNOLOGY0257-8972 THIN SOLID FILMS THIN SOLID FILMS0040-6090 APPL SURF SCI APPLIED SURFACE SCIENCE0169-4332 J THERM SPRAY TECHN JOURNAL OF THERMAL SPRAY TECHNOLOGY1059-9630 J VAC SCI TECHNOL A JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM 0734-2101 PROG ORG COAT PROGRESS IN ORGANIC COATINGS0300-9440 CORROS REV CORROSION REVIEWS0334-6005 INT J SURF SCI ENG International Journal of Surface Science and Eng1749-785X J COAT TECHNOL RES Journal of Coatings Technology and Research1547-0091 J PLAST FILM SHEET JOURNAL OF PLASTIC FILM & SHEETING8756-0879 JCT COATINGSTECH JCT COATINGSTECH1547-0083 KORROZ FIGY Korrozios Figyelo0133-2546 PIGM RESIN TECHNOL Pigment & Resin Technology0369-9420 SURF COAT INT SURFACE COATINGS INTERNATIONAL1754-0925 SURF ENG SURFACE ENGINEERING0267-0844 T I MET FINISH TRANSACTIONS OF THE INSTITUTE OF METAL FINISHING0020-2967 BIOMATERIALS BIOMATERIALS0142-9612 ACTA BIOMATER Acta Biomaterialia1742-7061 EUR CELLS MATER EUROPEAN CELLS & MATERIALS1473-2262 J MECH BEHAV BIOMED Journal of the Mechanical Behavior of Biomedical1751-6161 MACROMOL BIOSCI MACROMOLECULAR BIOSCIENCE1616-5187 BIOINTERPHASES Biointerphases 1559-4106 COLLOID SURFACE B COLLOIDS AND SURFACES B-BIOINTERFACES0927-7765 DENT MATER DENTAL MATERIALS0109-5641 J BIOACT COMPAT POL JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS0883-9115 J BIOMAT SCI-POLYM E JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION0920-5063 J BIOMED MATER RES A JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A1549-3296 J BIOMED MATER RES B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-1552-4973 ARTIF CELL BLOOD SUB ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZ1073-1199 BIOFABRICATION Biofabrication1758-5082 BIOINSPIR BIOMIM Bioinspiration & Biomimetics1748-3182 BIOMED MATER Biomedical Materials1748-6041 BIO-MED MATER ENG BIO-MEDICAL MATERIALS AND ENGINEERING0959-2989 CELL POLYM CELLULAR POLYMERS0262-4893 DENT MATER J DENTAL MATERIALS JOURNAL0287-4547 J APPL BIOMATER BIOM Journal of Applied Biomaterials & Biomechanics1722-6899 J BIOBASED MATER BIO Journal of Biobased Materials and Bioenergy1556-6560 J BIOMATER APPL JOURNAL OF BIOMATERIALS APPLICATIONS0885-3282 J BIONIC ENG Journal of Bionic Engineering1672-6529 J MATER SCI-MATER M JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICI0957-4530 MAT SCI ENG C-MATER Materials Science & Engineering C-Materials for 0928-4931 BIORESOURCES BioResources1930-2126 CELLULOSE CELLULOSE0969-0239 HOLZFORSCHUNG HOLZFORSCHUNG0018-3830 WOOD SCI TECHNOL WOOD SCIENCE AND TECHNOLOGY0043-7719 EUR J WOOD WOOD PROD European Journal of Wood and Wood Products0018-3768 J WOOD CHEM TECHNOL JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY0277-3813 J WOOD SCI JOURNAL OF WOOD SCIENCE1435-0211NORD PULP PAP RES J NORDIC PULP & PAPER RESEARCH JOURNAL0283-2631 TAPPI J TAPPI JOURNAL0734-1415 WOOD FIBER SCI WOOD AND FIBER SCIENCE0735-6161 APPITA J APPITA JOURNAL1038-6807 CELL CHEM TECHNOL CELLULOSE CHEMISTRY AND TECHNOLOGY0576-9787 DREWNO Drewno1644-3985 DRVNA IND Drvna Industrija0012-6772 FOREST PROD J FOREST PRODUCTS JOURNAL0015-7473 J PULP PAP SCI JOURNAL OF PULP AND PAPER SCIENCE0826-6220 MADERAS-CIENC TECNOL Maderas-Ciencia y Tecnologia 0717-3644 MOKUZAI GAKKAISHI MOKUZAI GAKKAISHI0021-4795 PAP PUU-PAP TIM PAPERI JA PUU-PAPER AND TIMBER0031-1243 PULP PAP-CANADA PULP & PAPER-CANADA0316-4004 WOCHENBL PAPIERFABR WOCHENBLATT FUR PAPIERFABRIKATION0043-7131 WOOD RES-SLOVAKIA WOOD RESEARCH1336-4561 ACS NANO ACS Nano 1936-0851 ADV FUNCT MATER ADVANCED FUNCTIONAL MATERIALS1616-301X ADV MATER ADVANCED MATERIALS0935-9648 ANNU REV MATER RES ANNUAL REVIEW OF MATERIALS RESEARCH1531-7331 MAT SCI ENG R MATERIALS SCIENCE & ENGINEERING R-REPORTS0927-796X MATER TODAY Materials Today1369-7021 NANO LETT NANO LETTERS1530-6984 NANO TODAY Nano Today1748-0132 NAT MATER NATURE MATERIALS1476-1122 NAT NANOTECHNOL Nature Nanotechnology1748-3387 PROG MATER SCI PROGRESS IN MATERIALS SCIENCE0079-6425 ACTA MATER ACTA MATERIALIA1359-6454 CARBON CARBON0008-6223 CHEM MATER CHEMISTRY OF MATERIALS0897-4756 CRIT REV SOLID STATE CRITICAL REVIEWS IN SOLID STATE AND MATERIALS SC1040-8436 CRYST GROWTH DES CRYSTAL GROWTH & DESIGN1528-7483 CURR OPIN SOLID ST M CURRENT OPINION IN SOLID STATE & MATERIALS SCIEN1359-0286 EXP MECH EXPERIMENTAL MECHANICS0014-4851 INT J PLASTICITY INTERNATIONAL JOURNAL OF PLASTICITY0749-6419 INT MATER REV INTERNATIONAL MATERIALS REVIEWS0950-6608 J MATER CHEM JOURNAL OF MATERIALS CHEMISTRY0959-9428 J MECH PHYS SOLIDS JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS0022-5096 J PHYS CHEM C Journal of Physical Chemistry C1932-7447 LANGMUIR LANGMUIR0743-7463 MICROSC MICROANAL MICROSCOPY AND MICROANALYSIS1431-9276 MRS BULL MRS BULLETIN0883-7694 NANO RES Nano Research1998-0124 NANOSCALE Nanoscale2040-3364 NANOTECHNOLOGY NANOTECHNOLOGY0957-4484 ORG ELECTRON ORGANIC ELECTRONICS1566-1199 PLASMONICS Plasmonics 1557-1955 SMALL SMALL1613-6810 SOFT MATTER Soft Matter1744-683X SOL ENERG MAT SOL C SOLAR ENERGY MATERIALS AND SOLAR CELLS0927-0248 THIN SOLID FILMS THIN SOLID FILMS0040-6090 ACS APPL MATER INTER ACS Applied Materials & Interfaces1944-8244 ADV ENG MATER ADVANCED ENGINEERING MATERIALS1438-1656APPL PHYS A-MATER APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING0947-8396 CEMENT CONCRETE RES CEMENT AND CONCRETE RESEARCH0008-8846 CMC-COMPUT MATER CON CMC-Computers Materials & Continua1546-2218 COMP MATER SCI COMPUTATIONAL MATERIALS SCIENCE0927-0256 CORROS SCI CORROSION SCIENCE0010-938X CURR APPL PHYS CURRENT APPLIED PHYSICS1567-1739 CURR NANOSCI Current Nanoscience1573-4137 DIAM RELAT MATER DIAMOND AND RELATED MATERIALS0925-9635 DIG J NANOMATER BIOS Digest Journal of Nanomaterials and Biostructure1842-3582 ELECTROCHEM SOLID ST ELECTROCHEMICAL AND SOLID STATE LETTERS1099-0062 EUR PHYS J E EUROPEAN PHYSICAL JOURNAL E1292-8941 FUNCT MATER LETT Functional Materials Letters1793-6047 GOLD BULL GOLD BULLETIN0017-1557 IEEE T NANOTECHNOL IEEE TRANSACTIONS ON NANOTECHNOLOGY1536-125X INT J ADHES ADHES INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES0143-7496 INT J DAMAGE MECH INTERNATIONAL JOURNAL OF DAMAGE MECHANICS1056-7895 INT J FATIGUE INTERNATIONAL JOURNAL OF FATIGUE0142-1123 INTERMETALLICS INTERMETALLICS0966-9795 J ALLOY COMPD JOURNAL OF ALLOYS AND COMPOUNDS0925-8388 J MATER RES JOURNAL OF MATERIALS RESEARCH0884-2914 J MATER SCI JOURNAL OF MATERIALS SCIENCE0022-2461 J MICROMECH MICROENG JOURNAL OF MICROMECHANICS AND MICROENGINEERING0960-1317 J NANOPART RES JOURNAL OF NANOPARTICLE RESEARCH1388-0764 J NANOSCI NANOTECHNO JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY1533-4880 J NON-CRYST SOLIDS JOURNAL OF NON-CRYSTALLINE SOLIDS0022-3093 J NUCL MATER JOURNAL OF NUCLEAR MATERIALS0022-3115 MACROMOL MATER ENG MACROMOLECULAR MATERIALS AND ENGINEERING1438-7492 MAT SCI ENG A-STRUCT MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL M0921-5093 MATER CHEM PHYS MATERIALS CHEMISTRY AND PHYSICS0254-0584 MATER LETT MATERIALS LETTERS0167-577X MATER RES BULL MATERIALS RESEARCH BULLETIN0025-5408 MATER SCI ENG B-ADV Materials Science and Engineering B-Advanced Fun0921-5107 MECH ADV MATER STRUC MECHANICS OF ADVANCED MATERIALS AND STRUCTURES1537-6494 MECH MATER MECHANICS OF MATERIALS0167-6636 METALL MATER TRANS A METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSI1073-5623 MICROPOR MESOPOR MAT MICROPOROUS AND MESOPOROUS MATERIALS1387-1811 NANOSCALE RES LETT Nanoscale Research Letters1931-7573 OPT MATER OPTICAL MATERIALS0925-3467 PHOTONIC NANOSTRUCT Photonics and Nanostructures-Fundamentals and Ap1569-4410 PHYS CHEM MINER PHYSICS AND CHEMISTRY OF MINERALS0342-1791 PHYS STATUS SOLIDI-R Physica Status Solidi-Rapid Research Letters1862-6254 SCI ADV MATER Science of Advanced Materials1947-2935 SCI TECHNOL ADV MAT SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS1468-6996 SCRIPTA MATER SCRIPTA MATERIALIA1359-6462 SMART MATER STRUCT SMART MATERIALS & STRUCTURES0964-1726 SOFT MATER SOFT MATERIALS1539-445X SYNTHETIC MET SYNTHETIC METALS0379-6779 WEAR WEAR0043-1648 ACI MATER J ACI MATERIALS JOURNAL0889-325X ACI STRUCT J ACI STRUCTURAL JOURNAL0889-3241 ACTA MECH SOLIDA SIN ACTA MECHANICA SOLIDA SINICA0894-9166 ADV CEM RES ADVANCES IN CEMENT RESEARCH0951-7197ADV MATER PROCESS ADVANCED MATERIALS & PROCESSES0882-7958 ANN CHIM-SCI MAT ANNALES DE CHIMIE-SCIENCE DES MATERIAUX0151-9107 ARCH CIV MECH ENG Archives of Civil and Mechanical Engineering1644-9665 ATOMIZATION SPRAY ATOMIZATION AND SPRAYS1044-5110 B MATER SCI BULLETIN OF MATERIALS SCIENCE0250-4707 CHALCOGENIDE LETT Chalcogenide Letters1584-8663 CIRCUIT WORLD CIRCUIT WORLD0305-6120 COMBUST EXPLO SHOCK+COMBUSTION EXPLOSION AND SHOCK WAVES0010-5082 CONSTR BUILD MATER CONSTRUCTION AND BUILDING MATERIALS0950-0618 CORROS ENG SCI TECHN CORROSION ENGINEERING SCIENCE AND TECHNOLOGY1478-422X CORROSION CORROSION0010-9312 ELECTRON MATER LETT Electronic Materials Letters1738-8090 FATIGUE FRACT ENG M FATIGUE & FRACTURE OF ENGINEERING MATERIALS & ST8756-758X FERROELECTRICS FERROELECTRICS0015-0193 FIBRE CHEM+FIBRE CHEMISTRY0015-0541 FIRE MATER FIRE AND MATERIALS0308-0501 FIRE SAFETY J FIRE SAFETY JOURNAL0379-7112 FIRE TECHNOL FIRE TECHNOLOGY0015-2684 FULLER NANOTUB CAR N FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES1536-383X GEOSYNTH INT GEOSYNTHETICS INTERNATIONAL1072-6349 GRANUL MATTER GRANULAR MATTER1434-5021 HIGH TEMP MAT PR-ISR HIGH TEMPERATURE MATERIALS AND PROCESSES0334-6455 HIGH TEMP MATER P-US HIGH TEMPERATURE MATERIAL PROCESSES1093-3611 IEEE T ADV PACKAGING IEEE TRANSACTIONS ON ADVANCED PACKAGING1521-3323 IEEE T COMPON PACK T IEEE TRANSACTIONS ON COMPONENTS AND PACKAGING TE1521-3331 INDIAN J ENG MATER S INDIAN JOURNAL OF ENGINEERING AND MATERIALS SCIE0971-4588 INFORM MIDEM INFORMACIJE MIDEM-JOURNAL OF MICROELECTRONICS EL0352-9045 INORG MATER+INORGANIC MATERIALS0020-1685 INT J FRACTURE INTERNATIONAL JOURNAL OF FRACTURE0376-9429 INT J MATER PROD TEC INTERNATIONAL JOURNAL OF MATERIALS & PRODUCT TEC0268-1900 INT J MIN MET MATER International Journal of Minerals Metallurgy and1674-4799 INT J NANOTECHNOL International Journal of Nanotechnology1475-7435 INT J NUMER ANAL MET INTERNATIONAL JOURNAL FOR NUMERICAL AND ANALYTIC0363-9061 INT J REFRACT MET H INTERNATIONAL JOURNAL OF REFRACTORY METALS & HAR0263-4368 INT J SURF SCI ENG International Journal of Surface Science and Eng1749-785X J ADHES SCI TECHNOL JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY0169-4243 J ADHESION JOURNAL OF ADHESION0021-8464 J ADV CONCR TECHNOL Journal of Advanced Concrete Technology 1346-8014 J ADV MATER-COVINA JOURNAL OF ADVANCED MATERIALS1070-9789 J COMPUT THEOR NANOS Journal of Computational and Theoretical Nanosci1546-1955 J CULT HERIT JOURNAL OF CULTURAL HERITAGE1296-2074 J ELASTICITY JOURNAL OF ELASTICITY0374-3535 J ELASTOM PLAST JOURNAL OF ELASTOMERS AND PLASTICS0095-2443 J ELECTRON MATER JOURNAL OF ELECTRONIC MATERIALS0361-5235 J ENERG MATER Journal of Energetic Materials 0737-0652 J ENG MATER-T ASME JOURNAL OF ENGINEERING MATERIALS AND TECHNOLOGY-0094-4289 J EXP NANOSCI Journal of Experimental Nanoscience1745-8080 J FIRE PROT ENG Journal of Fire Protection Engineering1042-3915 J FIRE SCI JOURNAL OF FIRE SCIENCES0734-9041 J FRICT WEAR+Journal of Friction and Wear1068-3666 J INTEL MAT SYST STR JOURNAL OF INTELLIGENT MATERIAL SYSTEMS AND STRU1045-389X J LASER MICRO NANOEN Journal of Laser Micro Nanoengineering1880-0688J MAGN MAGN MATER JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS0304-8853 J MATER CIVIL ENG JOURNAL OF MATERIALS IN CIVIL ENGINEERING0899-1561 J MATER ENG PERFORM JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE1059-9495 J MATER PROCESS TECH JOURNAL OF MATERIALS PROCESSING TECHNOLOGY0924-0136 J MATER SCI TECHNOL JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY1005-0302 J MATER SCI-MATER EL JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTR0957-4522 J MECH MATER STRUCT Journal of Mechanics of Materials and Structures1559-3959 J MICRO-NANOLITH MEM Journal of Micro-Nanolithography MEMS and MOEMS1932-5150 J NANO RES-SW Journal of Nano Research1662-5250 J NANOMATER Journal of Nanomaterials1687-4110 J NEW MAT ELECTR SYS JOURNAL OF NEW MATERIALS FOR ELECTROCHEMICAL SYS1480-2422 J OPTOELECTRON ADV M JOURNAL OF OPTOELECTRONICS AND ADVANCED MATERIAL1454-4164 J OVONIC RES Journal of Ovonic Research1584-9953 J PHASE EQUILIB DIFF JOURNAL OF PHASE EQUILIBRIA AND DIFFUSION1547-7037 J PHYS CHEM LETT Journal of Physical Chemistry Letters1948-7185 J POROUS MAT JOURNAL OF POROUS MATERIALS1380-2224 J SOC INF DISPLAY Journal of the Society for Information Display1071-0922 J SUPERHARD MATER+Journal of Superhard Materials 1063-4576 J WUHAN UNIV TECHNOL JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERI1000-2413 JOM-US JOM-JOURNAL OF THE MINERALS METALS & MATERIALS S1047-4838 KONA POWDER PART J KONA Powder and Particle Journal0288-4534 KOREAN J MET MATER Korean Journal of Metals and Materials1738-8228 KOVOVE MATER KOVOVE MATERIALY-METALLIC MATERIALS0023-432X LASER ENG LASERS IN ENGINEERING0898-1507 MACH SCI TECHNOL MACHINING SCIENCE AND TECHNOLOGY1091-0344 MAG CONCRETE RES MAGAZINE OF CONCRETE RESEARCH0024-9831 MAT SCI SEMICON PROC MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING1369-8001 MATER CONSTRUCC MATERIALES DE CONSTRUCCION0465-2746 MATER CORROS MATERIALS AND CORROSION-WERKSTOFFE UND KORROSION0947-5117 MATER DESIGN MATERIALS & DESIGN0261-3069 MATER HIGH TEMP MATERIALS AT HIGH TEMPERATURES0960-3409 MATER MANUF PROCESS MATERIALS AND MANUFACTURING PROCESSES1042-6914 MATER RES INNOV MATERIALS RESEARCH INNOVATIONS1432-8917 MATER RES-IBERO-AM J Materials Research-Ibero-american Journal of Mat1516-1439 MATER SCI TECH-LOND MATERIALS SCIENCE AND TECHNOLOGY0267-0836 MATER SCI+MATERIALS SCIENCE1068-820X MATER SCI-MEDZG Materials Science-Medziagotyra 1392-1320 MATER SCI-POLAND MATERIALS SCIENCE-POLAND0137-1339 MATER STRUCT MATERIALS AND STRUCTURES1359-5997 MATER TECHNOL MATERIALS TECHNOLOGY1066-7857 MATER TEHNOL Materiali in Tehnologije 1580-2949 MATER TRANS MATERIALS TRANSACTIONS1345-9678 MATER WORLD MATERIALS WORLD0967-8638 MATERIA-BRAZIL Materia-Rio de Janeiro1517-7076 MATERIALWISS WERKST MATERIALWISSENSCHAFT UND WERKSTOFFTECHNIK0933-5137 MATH MECH SOLIDS MATHEMATICS AND MECHANICS OF SOLIDS1081-2865 MET MATER INT METALS AND MATERIALS INTERNATIONAL1598-9623 METALL MATER TRANS B METALLURGICAL AND MATERIALS TRANSACTIONS B-PROCE1073-5615 METALLOFIZ NOV TEKH+METALLOFIZIKA I NOVEISHIE TEKHNOLOGII1024-1809 MICRO NANO LETT Micro & Nano Letters1750-0443 MICROELECTRON INT MICROELECTRONICS INTERNATIONAL1356-5362 MICROSYST TECHNOL MICROSYSTEM TECHNOLOGIES0946-7076MODEL SIMUL MATER SC MODELLING AND SIMULATION IN MATERIALS SCIENCE AN0965-0393 NANO NANO1793-2920 NEW CARBON MATER NEW CARBON MATERIALS1007-8827 OPTOELECTRON ADV MAT Optoelectronics and Advanced Materials-Rapid Com1842-6573 P I MECH ENG L-J MAT PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENG1464-4207 PARTICUOLOGY Particuology1674-2001 PHILOS MAG PHILOSOPHICAL MAGAZINE1478-6435 PHYS STATUS SOLIDI A PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIA1862-6300 PLAST ENG PLASTICS ENGINEERING0091-9578 PROG NAT SCI PROGRESS IN NATURAL SCIENCE1002-0071 RAPID PROTOTYPING J RAPID PROTOTYPING JOURNAL1355-2546 RARE METAL MAT ENG RARE METAL MATERIALS AND ENGINEERING1002-185X RARE METALS RARE METALS1001-0521 RECENT PAT NANOTECH Recent Patents on Nanotechnology1872-2105 REV ADV MATER SCI REVIEWS ON ADVANCED MATERIALS SCIENCE1606-5131 REV ROM MATER Revista Romana de Materiale-Romanian Journal of 1583-3186 ROAD MATER PAVEMENT Road Materials and Pavement Design1468-0629 SAMPE J SAMPE JOURNAL0091-1062 SCI CHINA TECHNOL SC Science China-Technological Sciences1674-7321 SCI MODEL SIMUL Scientific Modeling and Simulations1874-8554 SCI TECHNOL ENERG MA Science and Technology of Energetic Materials 1347-9466 SCI TECHNOL WELD JOI SCIENCE AND TECHNOLOGY OF WELDING AND JOINING1362-1718 SEMICOND SCI TECH SEMICONDUCTOR SCIENCE AND TECHNOLOGY0268-1242 SENSOR MATER SENSORS AND MATERIALS0914-4935 SOLDER SURF MT TECH SOLDERING & SURFACE MOUNT TECHNOLOGY0954-0911 T FAMENA Transactions of FAMENA 1333-1124 VACUUM VACUUM0042-207X ZKG INT ZKG INTERNATIONAL0949-0205 SIAM J IMAGING SCI SIAM Journal on Imaging Sciences1936-4954 IEEE T MED IMAGING IEEE TRANSACTIONS ON MEDICAL IMAGING0278-0062 REMOTE SENS ENVIRON REMOTE SENSING OF ENVIRONMENT0034-4257 INT J REMOTE SENS INTERNATIONAL JOURNAL OF REMOTE SENSING0143-1161 ISPRS J PHOTOGRAMM ISPRS JOURNAL OF PHOTOGRAMMETRY AND REMOTE SENSI0924-2716 PHOTOGRAMM ENG REM S PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING0099-1112 PHOTOGRAMM REC PHOTOGRAMMETRIC RECORD0031-868X EURASIP J IMAGE VIDE EURASIP Journal on Image and Video Processing1687-5176 IEEE J-STARS IEEE Journal of Selected Topics in Applied Earth1939-1404 IMAGING SCI J IMAGING SCIENCE JOURNAL1368-2199 INT J IMAG SYST TECH INTERNATIONAL JOURNAL OF IMAGING SYSTEMS AND TEC0899-9457 J APPL REMOTE SENS Journal of Applied Remote Sensing1931-3195 J ELECTRON IMAGING JOURNAL OF ELECTRONIC IMAGING1017-9909 J IMAGING SCI TECHN JOURNAL OF IMAGING SCIENCE AND TECHNOLOGY1062-3701 J REAL-TIME IMAGE PR Journal of Real-Time Image Processing1861-8200 PHOTOGRAMM FERNERKUN Photogrammetrie Fernerkundung Geoinformation1432-8364 RIV ITAL TELERILEVAM Rivista Italiana di Telerilevamento1129-8596 SIGNAL IMAGE VIDEO P Signal Image and Video Processing1863-1703 SMPTE MOTION IMAG J SMPTE MOTION IMAGING JOURNAL0036-1682 ANN FAM MED ANNALS OF FAMILY MEDICINE1544-1709 BRIT J GEN PRACT BRITISH JOURNAL OF GENERAL PRACTICE0960-1643 J AM BOARD FAM MED Journal of the American Board of Family Medicine1557-2625 SCAND J PRIM HEALTH SCANDINAVIAN JOURNAL OF PRIMARY HEALTH CARE0281-3432 AM FAM PHYSICIAN AMERICAN FAMILY PHYSICIAN0002-838X。

AR-A014418_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:AR–A014418 is a selective and effective GSK3β inhibitor with an IC 50 value of 104 nM, and has no significant inhibition on 26 other kinases.IC50 & Target: IC50: 104 nM (GSK3β)In Vitro: AR–A014418 inhibits tau phosphorylation at a GSK3–specific site (Ser–396) in 3T3 fibroblasts expressing human four–repeat tau protein with IC 50 of 2.7 μM, and protects cultured N2A cells from death induced by blocking PI3K/PKB pathway. In hippocampal slices, AR–A014418 inhibits neurodegeneration mediated by beta–amyloid peptide [1]. While in NGP and SH–5Y–SY cells,AR–A014418 reduces neuroendocrine markers and suppresses neuroblastoma cell growth [2].In Vivo: In ALS mouse model with the G93A mutant human SOD1, AR–A014418 (0–4 mg/kg, i.p.) delays the onset of symptoms,improves motor activity, slows down disease progression, and postpons the endpoint of the disease [3]. In addition, AR–A014418produces inhibition effect on acetic acid– and formalin–induced nociception in mice by modulating NMDA and metabotropic receptor signaling as well as TNF–α and IL–1β transmission in the spinal cord [4].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]The competition experiments are carried out in duplicate with 10 concentrations of the inhibitor inclear–bottomed microtiter plates. The biotinylated peptide substrate, biotin–AAEELDSRAGS(PO3H2)PQL, is added at a final concentration of 2 μM in an assay buffer containing 6 milliunits of recombinant human GSK3 (equal mix of both α and β), 12mM MOPS, pH 7.0, 0.3 mM EDTA, 0.01% β–mercaptoethanol, 0.004% Brij 35, 0.5% glycerol, and 0.5 μg of bovine serumalbumin/25 μL and preincubated for 10–15 min. The reaction is initiated by the addition of 0.04 μCi of [γ–33P]ATP and unlabeled ATP in 50 mM Mg(Ac)2 to a final concentration of 1 μM ATP and assay volume of 25 μL. Blank controls without peptide substrate are used.After incubation for 20 min at room temperature, each reaction is terminated by the addition of 25 μL of stop solution containing 5mM EDTA, 50 μM ATP, 0.1% Triton X–100, and 0.25 mg of streptavidin–coated SPA beads corresponding to appr 35 pmol of binding capacity. After 6 h the radioactivity is determined in a liquid scintillation counter. Inhibition curves are analyzed by non–linear regression using GraphPad Prism.Cell Assay: AR–A014418 is dissolved in DMSO.[1]Cell viability is assessed by calcein/propidium iodide uptake. Calcein AM is taken up and cleaved by esterases present within living cells, yielding yellowish–green fluorescence, whereas PI is only taken up by dead cells,which become orange–red fluorescent. In brief, N2A cells are cultured for 2 days in vitro and then treated with 50 μM LY–294002 in the presence of AR–A014418 or vehicle (DMSO) for 24 h. Subsequently, N2A cells are incubated for 30 min with 2 μM PI and 1 μM calcein–AM. The cultures are then rinsed three times with Hanks' buffered saline solution containing 2 mM CaCl 2, and the cells are visualized by fluorescence microscopy using a Zeiss Axiovert 135 microscope. Three fields (selected at random) are analyzed per well (appr 300 cells/field) in at least three different experiments. Cell death is expressed as percentage of PI–positive cells from the total number of cells. In every experiment, specific cell death is obtained after subtracting the number of dead cells present inProduct Name:AR–A014418Cat. No.:HY-10512CAS No.:487021-52-3Molecular Formula:C 12H 12N 4O 4S Molecular Weight:308.31Target:GSK–3; GSK–3Pathway:Stem Cell/Wnt; PI3K/Akt/mTOR Solubility:10 mM in DMSOvehicle–treated cultures.Animal Administration: AR–A014418 is formulated in normal saline.[3]First, to examine the effects of GSK–3 inhibition on the clinical symptoms, life span, and motor behavior function of ALS, 56 Tg mice are divided into four groups. In each group, 0.5 mL of normal saline is mixed with either 0 μg (control group), 1 μg (group A), 2 μg (group B) or 4 μg (group C) of AR–A014418 per gram of mouse, and injected intraperitoneally into 14 animals per group 5 days a week beginning 60 days after birth. The mice are sacrificed at the endpoint described below.References:[1]. Bhat R, Xue Y, Berg S, Structural insights and biological effects of glycogen synthase kinase 3–specific inhibitor AR–A014418. J Biol Chem. 2003 Nov 14; 278(46):45937–45.[2]. Carter YM, et al. Specific glycogen synthase kinase–3 inhibition reduces neuroendocrine markers and suppresses neuroblastoma cell growth. Cancer Biol Ther. 2014 May;15(5):510–5.[3]. Koh SH, et al. Inhibition of glycogen synthase kinase–3 suppresses the onset of symptoms and disease progression of G93A–SOD1 mouse model of ALS. Exp Neurol. 2007 Jun;205(2):336–46.[4]. Martins DF, et al. The antinociceptive effects of AR–A014418, a selective inhibitor of glycogen synthase kinase–3 beta, in mice. J Pain. 2011 Mar;12(3):315–22.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

血管外膜细胞钙化及其钙化机制研究

血管外膜细胞钙化及其钙化机制研究谭小青,张旭升,樊小容,黄战军摘要目的:研究经体外诱导钙化建立大鼠血管外膜细胞钙化模型,检测钙化过程中成骨相关指标及凋亡㊁自噬相关蛋白的表达变化,旨在为心血管疾病模型提供更精确的细胞模型,并初步探讨其钙化机制㊂方法:原代提取大鼠胸主动脉外膜纤维细胞,取3~6代细胞使用诱导培养基(高糖DMEM +10%胎牛血清+10mmol/L β-甘油磷酸+0.05mmol/L 抗坏血酸+100mmol/L 地塞米松)诱导钙化,诱导时间为3d ㊁6d ㊁9d ㊁12d ㊁15d ,筛选出诱导细胞钙化的最佳时间㊂对细胞采用茜素红S 染色㊁细胞内钙含量测定和碱性磷酸酶(ALP )活性检测,鉴定是否成功构建钙化模型㊂采用实时定量聚合酶链式反应(PT -PCR )检测成骨相关因子骨形态生成蛋白2(BMP2)和核心结合因子α1(Runx2)的mRNA 含量,蛋白免疫印迹法(Western Blot )检测凋亡蛋白Bax ㊁Bcl -2和自噬相关蛋白微血管相关蛋白(LC3)㊁Beclin -1的表达水平,找出血管外膜细胞钙化的潜在机制㊂结果:当诱导钙化时间为15d 时,血管外膜细胞中主要钙化指标胞内钙含量及ALP 活性上调(P <0.05),茜素红S 染色显示钙化组有明显钙盐沉积㊂血管外膜细胞经钙化诱导后,BMP2和Runx2的mRNA 水平上调,Bax 蛋白水平上调,Bcl -2和Beclin -1蛋白水平下调,LC3-Ⅱ/LC3-Ⅰ比值上调(P <0.05)㊂结论:钙化诱导培养基培养血管外膜细胞15d 可成功构建钙化细胞模型,血管外膜细胞钙化可能与细胞向成骨样表型转化有关,血管外膜细胞钙化过程涉及细胞自噬及凋亡调控㊂关键词血管外膜细胞;钙化;成骨样表型转化;自噬与凋亡;实验研究d o i :10.12102/j.i s s n .1672-1349.2023.18.010 Calcification of Vascular Adventitial Cells and Its MechanismTAN Xiaoqing,ZHANG Xusheng,FAN Xiaorong,HUANG Zhanjun Longgang District People 's Hospital of Shenzhen,Shenzhen 518172,Guangdong,China Corresponding Author ZHANG Xusheng,E -mail:*****************Abstract Objective:To investigate the mananism of calcification of rat vascular adventitial cells,establish the calcification model of rat vascular adventitial cells,and detect the expression changes of osteogenesis -related indicators,apoptosis,and autophagy -related proteins during the calcification process.It aimed to provide more accurate cell models for cardiovascular disease and initially explore the mechanism of calcification.Methods:Rat thoracic aortic adventitial fibroblasts were extracted from the primary generation,and the 3rd to 6th generation cells were used for induction medium(high glucose DMEM +10%fetal bovine serum +10mmol/L β-glycerophosphate +0.05mmol/L ascorbic acid +100mmol/L dexamethasone)to induce calcification,the induction time was 3,6,9,12,and 15d,and the optimal time for inducing cell calcification was selected.The cells were stained with alizarin red S,detected by intracellular calcium content and alkaline phosphatase(ALP)to identify whether the calcification model was successfully constructed.Real -time quantitative reverse transcription polymerase chain reaction(RT -PCR)was used to detect the mRNA levels of osteogenesis -related factors bone morphogenetic protein(BMP2)and runt -related transcription factor 2(Runx2);Western Blot was used to detect the apoptosis proteins Bax,Bcl -2,the autophagy -related proteins LC -3,and Beclin -1expression level;then the potential mechanism of vascular adventitial cell calcification would be revealed.Results:When calcification was induced for 15days,the intracellular calcium content in the adventitial cells of the main calcification indicators and ALP activity were up -regulated(P <0.05).Alizarin red S staining showed obvious calcium deposits in the calcification group.After calcification was induced in adventitial cells,the mRNA levels of BMP2and Runx2up -regulated,the protein levels of Bax up -regulated,the protein levels of Bcl -2and Beclin -1down -regulated,and the ratio of LC3-Ⅱ/LC3-Ⅰdown -regulated(P <0.05).Conclusion:Adventitial cells cultured in the calcification -inducing medium for 15days could successfully construct a calcified cell model.calcification of adventitial cells might be related to the transformation of cells to an osteoblast -like phenotype.The Calcification process of adventitial cells involved autophagy and apoptosis regulation.Keywords adventitial cells;calcification;osteogenic phenotype transformation;autophagy and apoptosis;experimental study血管钙化常见于动脉粥样硬化㊁血脂异常㊁高血压㊁糖尿病㊁慢性肾病及衰老等人群[1],血管钙化引起血管硬度增加㊁顺应性降低,导致心肌缺血㊁心力衰竭㊁血栓形成等,增加脑卒中㊁心脏病㊁动脉粥样硬化斑块破裂等的风险,被认为是影响心血管疾病的重要因素之一[2-4]㊂目前关于血管内膜㊁中膜和心脏瓣膜钙化的关注和研究相对较多㊂临床工作中发现,血管外膜也可发生钙化,然而调查发现,现阶段对血管外膜钙化的作者单位深圳市龙岗区人民医院(广东深圳518172)通讯作者张旭升,E -mail :*****************引用信息谭小青,张旭升,樊小容,等.血管外膜细胞钙化及其钙化机制研究[J ].中西医结合心脑血管病杂志,2023,21(18):3347-3350.关注较少,因此,需要更多的研究来阐明血管钙化的致病机制㊂最初血管钙化被认为是被动和退行性病变,标志着血管老化,但是越来越多研究表明血管钙化是类似于胚胎骨形成的病理生物学过程[5-6]㊂Bostr öm 等[7-8]研究发现,钙化过程中大鼠血管中膜细胞由原有收缩表型转变成为成骨样细胞表型,原有的收缩标志物如平滑肌肌动蛋白α(α-SMA )等表达减少,并表达核心结合因子α1(Runx2)㊁骨形态生成蛋白2(BMP2)等多种成骨样标志物,从而介导骨基质在血管中沉积㊂细胞凋亡与自噬为2种细胞死亡的方式,与血管钙化息息相关,研究表明,血管中膜细胞在细胞凋亡过程中释放凋亡小体,促进细胞钙化,而细胞自噬通过多种机制调控细胞钙化[9-10]㊂本研究对大鼠血管外膜细胞进行体外诱导钙化,建立大鼠血管外膜细胞钙化模型,并检测钙化过程中成骨相关指标及凋亡㊁自噬相关蛋白的表达变化,旨在为心血管疾病模型提供更精确的细胞模型,并初步探讨其钙化机制㊂1材料与方法1.1试剂胎牛血清(FBS,Gibco),青霉素,链霉素(Gibco,美国),茜素红S溶液,β-甘油磷酸,抗坏血酸,地塞米松(Sigma,美国),抗GAPDH抗体(Bioworld),抗Bcl-2, Bax,Bcelin1和微血管相关蛋白(LC3)抗体(CST),碱性磷酸酶检测试剂盒㊁钙(Ca)检测试剂盒(南京建城生物工程研究所)㊂1.2大鼠血管外膜细胞分离与培养取10只4~6周龄雄性Wistar-Kyoto大鼠(体质量120~180g)胸主动脉分离血管外膜,采用组织黏附法培养㊂使用添加10%胎牛血清的高糖DMEM培养基(Gibco dmem)在37ħ㊁5%二氧化碳条件下培养细胞㊂当细胞增殖至80%~90%融合时,用0.25%胰酶消化传代㊂使用第3代至第6代的细胞进行后续实验㊂1.3体外钙化模型的建立钙化诱导培养基为含10%胎牛血清,10mmol/L β-甘油磷酸钠,0.05mmol/L抗坏血酸和100mmol/L 地塞米松的高糖DMEM培养液㊂将第3代至第6代细胞分为对照组和钙化组,待细胞长至50%融合时,使用钙化诱导培养基培养,每3d更换1次培养基,连续培养15d㊂1.4碱性磷酸酶(ALP)酶活测定细胞钙化诱导后,弃去培养基,1ˑ磷酸缓冲盐溶液(PBS)洗细胞3次,加入裂解液500μL(1%T ritonX-100),冰上裂解40min后,离心,取上清液㊂使用上清液根据试剂盒说明书检测ALP活性及总蛋白含量㊂1.5细胞内钙含量检测细胞钙化诱导后,弃去培养基,1ˑPBS洗细胞3次,每孔加入500μL0.6mol/L的盐酸4ħ脱钙过夜,取上清,根据钙测试试剂盒说明书检测钙含量㊂将脱钙后的细胞用4ħPBS洗3次,每孔加入500μL NaOH/0.1%SDS裂解细胞,取上清,用二喹啉甲酸法(BCA)测定细胞蛋白含量㊂1.6茜素红S染色细胞钙化诱导15d,弃去培养基,1ˑPBS洗细胞3次,加入0.5mL4%多聚甲醛室温固定15min,用双蒸水洗3次,加入1mL0.1%茜素红室温孵育15min,吸去染液,双蒸水洗3次,在倒置显微镜下观察㊂1.7实时定量聚合酶链式反应(RT-PCR)检测细胞钙化诱导后,弃去培养基,1ˑPBS洗细胞3次,使用TaKaRa MiniBEST Universal RNA Extraction Kit提取总RNA,使用PrimeScrip TM RT reagent Kit将所提取的RNA逆转录合成cDNA,以cDNA为模板,通过SYBR Green I嵌合荧光定量RT-PCR检测BMP-2㊁Runx2和GAPDH的表达量㊂引物序列见表1㊂表1引物序列基因方向序列Runx2正向5'-TGGCTTTGGTTTCAGGTTAGG-3'反向5'-TGGAGATGTTGCTCTGTTCG-3' BMP-2正向5'-TGAGGATTAGCAGGTCTTTGC-3'反向5'-TCTCGTTTGTGGAGTGGATG-3' GAPDH正向5'-GGCTGCCCAGAACATCAT-3'反向5'-CGGACACATTGGGGGTAG-3'1.8蛋白免疫印迹法(Western Blot)检测细胞钙化诱导15d,弃去培养基,1ˑPBS洗细胞3次,提取细胞总蛋白㊂使用12%SDS-PAGE胶电泳分离,并转移到聚偏二氟乙烯膜(PVDF)上,封闭后,加入一抗(Bax1ʒ1000,Bcl-21ʒ1000,Beclin11ʒ1000, LC31ʒ1000,GAPDH1:1000)稀释液,4ħ孵育过夜;加入二抗稀释液(1ʒ10000)室温孵育1h后,使用ECL发光试剂盒显影并计算灰度值㊂1.9统计学处理应用SPSS19.0软件进行统计处理,符合正态分布的定量资料以均数ʃ标准差(xʃs)表示,比较采用t检验,以P<0.05为差异有统计学意义㊂2结果2.1大鼠血管外膜细胞可在体外被诱导钙化为验证高磷是否能诱导大鼠血管外膜细胞钙化,使用钙化诱导培养基培养细胞,在不同时间点检测ALP活性和胞内钙含量㊂随着培养时间延长,ALP活性逐渐上升,在培养第12天达到峰值,与对照组比较差异有统计学意义(P<0.05);诱导第3天开始所测得的胞内钙含量与对照组比较升高(P<0.05),ALP 活性和钙含量升高具有时间依赖性㊂详见图1㊁图2㊂诱导15d所测得钙含量最高,因此,后续实验选择的诱导时间为15d㊂对钙化诱导15d的细胞进行茜素红S染色,结果显示,对照组细胞呈长梭形,而钙化组细胞变成菱形㊂茜素红S染色后,钙化组可观察到大量的橘红色钙结节(见图3),而对照组完全没有㊂这也证明大鼠血管外膜细胞可在体外被钙化培养基诱导钙化㊂图1钙化诱导培养基诱导外膜细胞后ALP含量(与0d时比较,*P<0.05)图2钙化诱导培养基诱导外膜细胞后胞内钙含量(与0d时比较,*P<0.05)图3培养15d时细胞经茜素S红染色切片图(ˑ100)2.2血管外膜细胞钙化与细胞向成骨样表型转化有关血管钙化的增加与成骨细胞特异性标志物如BMP2㊁和Runx2的增加有关[11]㊂RT-PCR结果显示,与对照组比较,钙化组的成骨细胞特异性标志物BMP2和Runx2mRNA表达量增加,与对照组比较差异有统计学意义(P<0.05)㊂详见图4㊂图4外膜细胞钙化过程中BMP2和Runx2mRNA表达量(与对照组比较,*P<0.05)2.3血管外膜细胞钙化过程涉及细胞自噬及凋亡调控通过Western Blot检测凋亡和自噬相关蛋白的表达量变化㊂与对照组比较,钙化组促凋亡蛋白Bax表达上调,抑凋亡蛋白Bcl-2表达下调(P<0.05)㊂详见图5㊂钙化组自噬相关蛋白Beclin1表达上调,LC3-Ⅱ/ LC3-Ⅰ比例上调(P<0.05),说明钙化诱导培养后细胞内凋亡水平上调㊁自噬水平升高㊂详见图6㊂图5诱导钙化后促凋亡蛋白及抑凋亡蛋白表达变化图6诱导钙化后凋亡及自噬蛋白Beclin1等表达变化3讨论血管钙化作为心血管疾病病人的并发症之一,其发病率与严重程度逐年增高及加重,是导致心血管疾病病人高死亡率的重要因素㊂血管钙化缺乏有效的治疗药物㊂因此,探究血管钙化发病机制,在分子水平寻找有效的诊断和防治靶点是急需开展的基础研究工作㊂本研究证明,使用10mmol/Lβ-甘油磷酸+0.05 mmol/L抗坏血酸+100mmol/L地塞米松培养外膜细胞即可诱导大鼠血管外膜细胞在体外发生钙化,这是通过茜素红S染色㊁ALP活性检测及胞内钙含量检测结果得以确定的㊂血管钙化过程中,血管中膜细胞向成骨样细胞表型转变并表达相关成骨标志物,从而引起骨基质的沉积,是血管钙化的重要特点及机制[5]㊂本实验所用的血管外膜细胞钙化条件与血管中膜细胞钙化条件一致,说明血管外膜细胞钙化的机制可能与中膜细胞钙化的机制部分一致㊂血管中膜细胞钙化过程中,细胞表达成骨相关的转录因子如Runx2等,进而促进下游表达骨相关蛋白如骨形态发生蛋白BMP2等的表达,从而促使细胞向成骨样细胞主动分化[12-13],本研究也观察到类似的机制㊂通过PT-PCR检测,发现钙化培养基培养大鼠血管外膜细胞15d后,BMP2和Runx2的mRNA表达水平升高㊂本研究通过对钙盐沉积与成骨样细胞表型转变2个维度的探讨,证明血管外膜细胞可在体外被诱导钙化,丰富了血管钙化的分型㊂血管钙化的发生机制复杂,涉及多种信号通路,如细胞自噬和凋亡㊁Wnt/β-catenin信号通路激活㊁内质网应激等均参与调控血管钙化的过程㊂自噬作为一种细胞应激的适应性反应,在维持血管结构与功能中十分关键㊂研究表明,血管钙化过程中自噬水平增高[14-15]㊂在体外实验中,高磷可提高大鼠血管中膜细胞的自噬水平,增加细胞内自噬体数量,从而抑制凋亡与钙化[16]㊂还有研究表明,自噬可通过抑制大鼠血管中膜细胞氧化应激,抑制血管内皮细胞的炎症反应,对三酰甘油等脂代谢进行调控,从而减轻血管钙化[17-18]㊂LC3和Beclin1是2种典型的自噬标志物,Western Blot实验结果表明,用钙化培养基诱导大鼠血管外膜细胞15d,LC3-Ⅱ/LC3-Ⅰ比率升高,Beclin1蛋白水平表达升高,说明细胞内自噬水平升高㊂多项研究表明,细胞凋亡参与促进血管钙化的发生,抑制细胞凋亡和抑制钙化[16-17]㊂在对大鼠的体内研究发现,成纤维细胞生长因子21通过内质网应激调控Caspase-12信号通路来减少血管内中膜细胞凋亡,从而抑制血管钙化[18]㊂另外,提高培养基中的Pi 或Ca2+浓度,可诱导细胞质膜形成并释放基质囊泡(如凋亡小体),从而导致细胞外基质钙化,这种基质钙化可能成为血管钙化的成核位点[19]㊂Bax和Bcl-2是2种典型的凋亡和抑制凋亡蛋白,本实验结果证明,利用钙化培养基对血管外膜细胞诱导钙化过程中,细胞内凋亡水平升高㊂同时细胞内自噬水平也升高,这可能是细胞自我调控以对抗钙化的结果㊂本研究证实血管外膜细胞可在体外被诱导钙化,且外膜钙化过程与骨组织钙化过程类似,为主动可调控的过程㊂血管钙化是一个复杂的过程,涉及细胞凋亡和自噬等调控通路,仍需进一步研究㊂参考文献:[1]梁英权,段亚君,韩际宏.血管钙化分子机制研究进展[J].中国动脉硬化杂志,2020,28(11):921-929.[2]NICOLL R,HENEIN M Y.The predictive value of arterial andvalvular calcification for mortality and cardiovascular events[J].Int J Cardiol Heart Vessel,2014,3:1-5.[3]JOHNSON R C,LEOPOLD J A,LOSCALZO J.Vascularcalcification:pathobiological mechanisms and clinical implications[J].Circulation Research,2006,99(10):1044-1059.[4]YAMADA S,GIACHELLI C M.Vascular calcification in CKD-MBD:roles for phosphate,FGF23,and Klotho[J].Bone,2017,100:87-93.[5]LIN M E,CHEN T M,WALLINGFORD M C,et al.Runx2deletion insmooth muscle cells inhibits vascular osteochondrogenesis andcalcification but not atherosclerotic lesion formation[J].Cardiovascular Research,2016,112(2):606-616.[6]DURHAM A L,SPEER M Y,SCATENA M,et al.Role of smoothmuscle cells in vascular calcification:implications in atherosclerosis andarterial stiffness[J].Cardiovascular Research,2018,114(4):590-600.[7]BOSTRÖM K I,RAJAMANNAN N M,TOWLER D A.The regulationof valvular and vascular sclerosis by osteogenic morphogens[J].Circulation Research,2011,109(5):564-577.[8]SPEER M Y,YANG H Y,BRABB T,et al.Smooth muscle cells giverise to osteochondrogenic precursors and chondrocytes incalcifying arteries[J].Circulation Research,2009,104(6):733-741.[9]PROUDFOOT D,SKEPPER J N,HEGYI L,et al.Apoptosisregulates human vascular calcification in vitro:evidence forinitiation of vascular calcification by apoptotic bodies[J].Circulation Research,2000,87(11):1055-1062.[10]AN S J,BOYD R,ZHU M,et al.NADPH oxidase mediatesangiotensin II-induced endothelin-1expression in vascularadventitial fibroblasts[J].Cardiovascular Research,2007,75(4):702-709.[11]ZEADIN M,BUTCHER M,WERSTUCK G,et al.Effect of leptin onvascular calcification in apolipoprotein E-deficient mice[J].Arterioscler Thromb Vasc Biol,2009,29(12):2069-2075. [12]LEOPOLD J A.Vascular calcification:mechanisms of vascularsmooth muscle cell calcification[J].Trends in CardiovascularMedicine,2015,25(4):267-274.[13]刘聿秀.高尿酸诱导血管钙化的机制研究[D].青岛:青岛大学,2015.[14]LIU Q,LUO Y,ZHAO Y,et al.Nano-hydroxyapatite acceleratesvascular calcification via lysosome impairment and autophagydysfunction in smooth muscle cells[J].Bioact Mater,2022,8:478-493.[15]LIANG J,HUANG J,HE W,et al.β-Hydroxybutyric Inhibits vascularcalcification via autophagy enhancement in models induced byhigh phosphate[J].Front Cardiovasc Med,2021,8:685748. [16]CICERI P,ELLI F,CAPPELLETTI L,et al.A new in vitro model todelay high phosphate-induced vascular calcification progression[J].Mol Cell Biochem,2015,410(1/2):197-206.[17]BYON C H,JAVED A,DAI Q,et al.Oxidative stress inducesvascular calcification through modulation of the osteogenictranscription factor Runx2by AKT signaling[J].The Journal ofBiological Chemistry,2008,283(22):15319-15327.[18]OUIMET M,FRANKLIN V,MAK E,et al.Autophagy regulatescholesterol efflux from macrophage foam cells via lysosomal acidlipase[J].Cell Metabolism,2011,13(6):655-667.[19]REYNOLDS J L,JOANNIDES A J,SKEPPER J N,et al.Humanvascular smooth muscle cells undergo vesicle-mediatedcalcification in response to changes in extracellular calcium andphosphate concentrations:a potential mechanism for acceleratedvascular calcification in ESRD[J].Journal of the AmericanSociety of Nephrology,2004,15(11):2857-2867.(收稿日期:2022-03-30)(本文编辑王雅洁)。

ABT-737_SDS_MedChemExpress

Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :ABT-737Catalog No. :HY-50907CAS No. :852808-04-91.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:ABT737; ABT 737Formula:C42H45ClN6O5S2Molecular Weight:813.43CAS No. :852808-04-94. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

BIBR_1532_LCMS_07178_MedChemExpress

=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 89Acq. Instrument : HY-LCMS-02 Location : Vial 77Injection Date : 12/22/2014 5:12:06 PM Inj : 1Inj Volume : 3.000 µlAcq. Method : D:\AGLIENT 1260\DATA\20141222\20141222 2014-12-22 09-20-15\100-1000MS+3MIN( 0.02%FA).MLast changed : 12/22/2014 9:20:15 AM by Li Shan(LCMS-02)Analysis Method : D:\AGLIENT 1260\METHOD\HM-046_6-H01P.M Last changed : 12/22/2014 5:45:46 PM by Li Shan(LCMS-02) (modified after loading) M ethod Info : HPLCCatalog No : HY-17353 Batch#07178 A-RP-32Additional Info : Peak(s) manually integratedmin0.511.52 2.53mAU 02004006008001000120014001600 DAD1 C, Sig=254,4 Ref=off (D:\AGLIENT...0\DATA\20141222\20141222 2014-12-22 09-20-15\CPK2014-D22-07178.D)1.9422.0852.359===================================================================== Area Percent Report =====================================================================Sorted By : Signal Multiplier : 1.0000Dilution : 1.0000Do not use Multiplier & Dilution Factor with ISTDsSignal 1: DAD1 C, Sig=254,4 Ref=offPeak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 1.942 MM R 0.0455 5.10748 1.87211 0.1011 2 2.085 MM R 0.0464 5029.92920 1808.31567 99.5461 3 2.359 MM R 0.0439 17.82766 6.76513 0.3528Totals : 5052.86434 1816.95291===================================================================== *** End of Report ***=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 89Acq. Instrument : HY-LCMS-02 Location : Vial 77Injection Date : 12/22/2014 5:12:06 PM Inj : 1Inj Volume : 3.000 µlAcq. Method : D:\AGLIENT 1260\DATA\20141222\20141222 2014-12-22 09-20-15\100-1000MS+3MIN( 0.02%FA).MLast changed : 12/22/2014 9:20:15 AM by Li Shan(LCMS-02)Analysis Method : D:\AGLIENT 1260\METHOD\HM-046_6-H01P.M Last changed : 12/22/2014 5:46:59 PM by Li Shan(LCMS-02) (modified after loading) M ethod Info : HPLCCatalog No : HY-17353 Batch#07178 A-RP-32Additional Info : Peak(s) manually integratedmin0.511.522.53250005000075000100000125000150000175000200000 MSD1 TIC, MS File (D:\AGLIENT 1260\DATA\20141222\20141222 2014-12-22 09-20-15\CPK2014-D22-07178.D) ES-API, Pos, S2.085MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 50 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts.Reportable Ion Abundance: > 10%.Retention Mol. Weight Time (MS) MS Area or Ion2.085 1138752 354.05 I 196.10 I 195.10 I 124.15 Im/z10020030040050060020406080100*MSD1 SPC, time=2.054:2.145 of D:\AGLIENT 1260\DATA\20141222\20141222 2014-12-22 09-20-15\CPK2014-D22-07178.D ES-API Max: 67215101.2282.3196.1354.0124.2195.1*** End of Report ***。

Expression, Purification and Crystallization of

Expression, Purification and Crystallization of the Mycobacterium Tuberculosis HSP16.3 Molecular Chaperone Background of Mycobacterium Tuberculosis HSP16.3HSP16.3, a 16.3 kDa protein from Mycobacterium Tuberculosis, was originally identified as a prominent antigen (Kingston et al., 1987). During the stationary phase, HSP16.3 is maximally expressed and becomes a main protein of the latent phase (Yuan et al., 1996). Previous studies showed that HSP16.3 can make the cell structure stable and prevent stationary Mycobacterium Tuberculosis from autolysing (Cunningham et al., 1998). In previous studies, HSP16.3 was found as one of theα-crystallin-related small heat shock proteins (sHSP) with molecular chaperone activity. Experiments in vitro revealed that HSP16.3 can suppress the thermal aggregation of citrate synthase at 39.5˚C, without consumption of A TP (Chang et al., 1996).Now the Mycobacterium Tuberculosis HSP16.3 gene was cloned to the plasmid pSTE-HSP16.3, and transformed to E.Coli. BL21(DE3) strain.Material and MethodExpressionThings to have ready before Starting.-Plate or glycerol culture-Sterile LB 25ml in a 50mL shaker flasker, 250ml in a 500mL shaker flasker, all together autoclaved, antibiotic added afterword.- antibiotic and sterile water- TipsPrepare the LB and autoclave:Fomula of the LB medium for 1 Liter:Bacto Tryptone (BT) 10 gBacto Y east Extract (BYE) 10 gNaCl 10gThe LB medium, dd H2O and the tips all together autoclaved at 121 ˚C for 20 minutes.Method:1 Innoculate 25 ml LB Medium ( containing 100 ug) and grow culture overnight(37˚C, 200rpm).2 Next morning inoculate 250 ml prewarmed LB Medium ( containing 100 ug) with the 25 ml overnight culture and grow at 37 ˚C, 200rpm, HSP16.3 was overexpressed in soluble form intracellularly without IPTG induction.3 Incubate the Culture for 10 hours before havesting the cell at 4000 g for 20 minutes.4 Resuspend the cell pellet in 30 ml Butter A and freeze the Sample in -80˚C refigerator.PurificationDE52 Ion-Exchange columnThings to have ready before Starting.-Butter A: 50 mM Imidazole pH 6.5 (1 liter)-Butter B: 50 mM Imidazole pH 6.5 , 300mM NaClall together Fitrate with 0.2 um membrane.- DE52 medium , column ,Gradient maker, UV-monitor and Fractioner- TipsMethod:1 Thaw the cell pellet and vortex .2 Add 0.4ml 100 mM PMSF and sonicate (400kw, 4s-6s 50 cycle* 5 )3 Centrifuge 15000 rpm, 30 minutes to pellet debris4 Transfer supernatant to a 50 ml conicale tube and discard the pellet.5 The supernatant dilute to 50 ml with Buffer A and then load to DE52 ion-exchange columns (20ml), which was pre-equibrated with 100ml Buffer A. And then wash the unbound proteins with 100 ml Buffer A.6 Elute the protein with a linear gradient : 200ml buffer A plus 200ml buffer B, 2ml/min, 6ml each fraction.7 Run 15% SDS-PAGE to determine the HSP16.3 peak.Desalting by dialysis1 Preparation of the dialysis tubeCut the tube in a suitable length (20-30 cm)Boil the tube in solution containing 10 mM NaHCO3 for a few minutes.Boil the tube in solution containing 10 mM EDTA for a few minutes.Rasin the tube with de-ion water2 Pool the HSP16.3 peak and dialysis the Sample against 1000ml Buffer A for more than 6hours.Q-Separose (HP) Ion-Exchange Column1 load the sample to Q-Separose (HP) Ion-Exchange column (20ml), which was pre-equibrated with 100ml Buffer A. And then wash the unbound proteins with 100 ml Buffer A.2 Elute the protein with a linear gradient : 200ml buffer A plus 200ml buffer B, 2ml/min, 6ml each fraction.3 Run 15% SDS-PAGE to determine the purity of the HSP16.3 peak.Gel filtration ColumnThe HSP peak was a final volumn 0.3ml and then run though a Superdex75 (HR, 10/30mm) gel filtration column in 150mM NaCl and 5mM Imdazole, pH6.5. Crystallization1 The purified HSP16.3 was solvent-exchanged to water and concentrated to 20mg/ml before crystallization trails (Bradford). All the crystallization trials were carried out using the hanging-drop vapor-diffusion method at 291K: drops consisted of2 microlitres of HSP16.3 protein solution plus 2 microlitres of the precipitant. The drops were equilibrated against 0.2 ml precipitant at room temperature. The crystallization conditions were investigated with a PEG4000 Kit.Result and discussionThe purity of the final HSP16.3 was over 95% by SDS-PAGE. The crystallization trials of HSP16.3 yielded Cubic crystals with a size of 0.8*0.8*0.6mm in a few days.20040060080010001200mAUBuffer Tris-HCL pH 8.5 Precipitant PEG 4000 MethodV apor Diffusion Temperature 293 K Size0.8*0.8*0.6mmReferencesChang Z., Primm, T.P., Jakana J., Lee H. I., Serysheva I., Chiu W., Gilber H. F., Quiocho F. A., (1996) J Biol Chem 271:7218-7223Cunningham A. F., Spreadbury C. L., (1998) J. Bacteriol. 184:801-808Kingston A. E., Salgame P. R., Mitchison N.A., Colston M. J. (1987) Infect. Immun 55,3149-3154Yuan Y., Crane D. D., Barry C. E. III (1996) J Bacteriol178: 4484-4492。

1,4,5-三磷酸肌醇受体与神经变性疾病

1，4，5-三磷酸肌醇受体与神经变性疾病赵吉利1，岳雅蓉1，张鑫1，杜文倩1，王云霞1综述，薛慧2，项文平2，孟天予2审校摘要：1，4，5-三磷酸肌醇受体（inositol 1，4，5-trisphosphate receptors，IP3Rs）是细胞内质网上的钙离子（cal‑cium ion，Ca2+）通道，通过调控Ca2+参与细胞生物学功能，是维持中枢神经系统正常功能的关键分子。

近年来，越来越多的研究发现，IP3Rs结构和功能异常与神经变性疾病如阿尔茨海默病、帕金森病、亨廷顿病、脊髓小脑共济失调等的发病机制密切相关，这些结构和功能异常如何影响IP3Rs功能，及相关钙信号，并且如何在这些疾病的发病和严重程度中发挥作用，仍尚不清楚。

IP3Rs如何在神经变性疾病中发挥作用将于本文中进行综述。

关键词：1，4，5-三磷酸肌醇受体；钙离子；神经变性疾病；认知障碍中图分类号：R741 文献标识码：AInositol 1，4，5-trisphosphate receptors and neurodegenerative diseases ZHAO Jili，YUE Yarong，ZHANG Xin，et al.（Central School of Clinical Medicine，Baotou Medical College，Inner Mongolia University of Science and Technology，Baotou 014040，China）Abstract：Inositol 1，4，5-trisphosphate receptor （IP3R），which is a calcium ion （Ca2+） channel in the endoplasmic re‑ticulum，participates in cellular biological functions through regulating the Ca2+ signal，and it is a key molecule in maintaining the normal function of the central nervous system. In recent years，more and more studies have found that the structural and functional abnormalities of IP3Rs are closely related to the pathogenesis of neurodegenerative diseases such as Alzheimer's disease，Parkinson disease，Huntington disease，and spinocerebellar ataxia. However，it remains unclear how these structural and functional abnormalities affect the function of IP3Rs and the related calcium signal as well as the pathogenesis and sever‑ity of neurodegenerative diseases. This paper reviews the role of IP3Rs in neurodegenerative diseases.Key words：Inositol 1，4，5-trisphosphate receptor；Calcium ion；Neurodegenerative disease；Cognitive disorder1，4，5-三磷酸肌醇受体（inositol 1，4，5-trisphos‑phate receptors，IP3Rs）是一种位于内质网上的配体门控的Ca2+通道，1998年Supattapone等人［1］首次在大鼠小脑中发现IP3Rs，其广泛表达于单细胞原生动物在内的动物细胞中，通过调节内质网中Ca2+的释放，产生钙信号。

中科院SCI分区目录

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刊名简称 J AM CHEM SOC ANGEW CHEM INT EDIT ANAL CHEM CHEM REV ACCOUNTS CHEM RES COORDIN CHEM REV CHEM SOC REV ANNU REV PHYS CHEM PROG POLYM SCI TOP CURR CHEM ADV POLYM SCI SURF SCI REP CATAL REV CURR OPIN COLLOID IN ADV CATAL ADV ORGANOMET CHEM PROG SOLID STATE CH ALDRICHIM ACTA J ORG CHEM MACROMOLECULES CHEM COMMUN INORG CHEM J PHYS CHEM B LANGMUIR J CHROMATOGR A ORGANOMETALLICS DALTON T J CATAL ORG LETT CHEM-EUR J ELECTROPHORESIS J COMPUT CHEM CARBON RAPID COMMUN MASS SP CHEM RES TOXICOL J ANAL ATOM SPECTROM APPL CATAL B-ENVIRON MACROMOL RAPID COMM J AM SOC MASS SPECTR J PHYS CHEM REF DATA FARADAY DISCUSS J MASS SPECTROM ADV COLLOID INTERFAC BIOMACROMOLECULES TRAC-TREND ANAL CHEM ELECTROCHEM COMMUN J BIOL INORG CHEM ADV SYNTH CATAL ADV INORG CHEM CHEMPHYSCHEM GREEN CHEM J COMB CHEM PROG SURF SCI CRYST GROWTH DES

15523666_迷迭香酸对哮喘小鼠氧化性肺损伤的保护作用

$ 材料与方法
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号!D#2*%桂'"8!$?888"# 试验前适应性饲养:P" 自由饮水饮食"饲养环境温度 "$ c l! c"相对湿度 $85 "085 # !3!3" 主要试剂和药品 9,D$D,'$1DZ?CQ 检测试剂盒均购自碧云天生物技术研究所*,(; 购自 DEX\@ 公司*液态铝佐剂购自 >OHM\I 公司# 肿节风药材购自广西玉林中桂药材有限公司"药材
%!3广西大学动物科学技术学院"南宁 4:8884*"3广西畜牧研究所"南宁 4:888!'
摘要为评价迷迭香酸对哮喘小鼠模型氧化性肺损伤的保护作用"本研究用卵清蛋白 %,(;'致敏$激发雌性 &;B&&S小鼠建立哮喘模型"并用 ,(; 和 Z"," 联合激发小鼠作为氧化肺损伤阳性对照模型#在最后一次滴鼻激发 "$O 后 "取支气管肺泡灌洗液 %&;B['进行细胞计数并测定活性氧 %9,D'$超氧化物歧化酶 %D,''和谷胱甘肽过氧化物酶%1DZ?CQ'水平"取左侧肺脏固定做 Z= 染色#结果显示"迷迭香酸可明显减少 &;B[ 中细胞总数和嗜酸性粒细胞数目"显著抑制肺组织和 &;B[ 中 9,D的产生"升高 D,' 和 1DZ?CQ水平"改善肺组织病理变化# 本试验结果表明 "迷迭香酸对氧化肺损伤起到明显的保护作用 # 关键词迷迭香酸 *哮喘 *氧化性肺损伤 *抗氧化中图分类号 9"0434 文献标识码 ; 文章编号 !+6!?6":+%"8!6'!"?:+48?8+

吡唑衍生物合成、生物活性及罗丹明B类pH荧光探针

吡唑衍生物合成、生物活性及罗丹明B类pH荧光探针一、本文概述本文旨在全面探讨吡唑衍生物的合成方法、生物活性及其在化学和生物领域的应用。

本文还将深入研究罗丹明B类pH荧光探针的性质和应用，以期在分子探针设计和生物检测方面提供新的思路和方法。

我们将详细介绍吡唑衍生物的合成策略，包括原料选择、反应条件优化以及产物分离和纯化等步骤。

通过对合成路线的系统研究，我们期望发现更加高效、环保的合成方法，为吡唑衍生物的大规模生产和应用奠定基础。

我们将对吡唑衍生物的生物活性进行深入研究。

通过体外和体内实验，评估吡唑衍生物在抗菌、抗病毒、抗肿瘤等方面的生物活性，揭示其作用机制和潜在的药用价值。

这些研究不仅有助于拓宽吡唑衍生物的应用领域，还为药物研发和生物治疗提供新的候选分子。

本文还将对罗丹明B类pH荧光探针进行详细阐述。

我们将从探针的设计原理、合成方法、光谱性质、pH响应特性等方面展开研究，以期发现具有高灵敏度、高选择性和快速响应的pH荧光探针。

这些探针在生物检测、环境监测和药物研发等领域具有广泛的应用前景。

本文旨在全面研究吡唑衍生物的合成、生物活性及其在化学和生物领域的应用，同时深入探讨罗丹明B类pH荧光探针的性质和应用。

我们期望通过本文的研究，为相关领域的发展提供新的思路和方法，推动化学和生物科学的进步。

二、吡唑衍生物的合成吡唑衍生物是一类具有广泛生物活性的化合物，其合成方法多种多样，主要包括直接合成法、重排反应法以及多组分反应法等。

直接合成法通常是通过羧酸与肼或酰肼在加热条件下发生缩合反应来制备吡唑衍生物。

这种方法简单易行，是实验室中最常用的合成方法之一。

在反应过程中，需要选择合适的溶剂和温度，以确保反应的顺利进行。

重排反应法则是通过某些特定结构的化合物在加热或催化剂的作用下发生分子内重排反应，从而得到吡唑衍生物。

这种方法通常需要较高的温度和较长的反应时间，但对于某些特定结构的吡唑衍生物合成具有独特的优势。

多组分反应法是一种高效、快速的合成方法，它可以在一步反应中同时形成多个化学键，从而得到目标产物。

Bioorg. Med. Chem. Lett. 17 (2007) 3317-3321

Design and synthesis of urea and thiourea derivatives and their inhibitory activities on lipopolysaccharide-inducedNO productionYoon Jung Kim,Jae-Ha Ryu,Ye Jin Cheon,Hyo Jin Lim and Raok Jeon *College of Pharmacy,Sookmyung Women’s University,52Hyochangwon-Gil,Yongsan-Ku,Seoul 140-742,Republic of KoreaReceived 17November 2006;revised 20March 2007;accepted 2April 2007Available online 6April 2007Abstract—Series of ureas and thioureas were designed and synthesized,and their inhibitory activities of NO production in lipopoly-saccharide-activated macrophages were evaluated.We found several essential moieties in the structure of the prepared compounds for the activity.Thiourea derivatives revealed higher inhibitory activity than the corresponding urea derivatives.Among these com-pounds,7e having carboxymethyl group at N3position of thiourea was the most potent in the inhibition of NO production.They inhibited NO production through the suppression of iNOS protein and mRNA expression.Ó2007Elsevier Ltd.All rights reserved.The critical role of nitric oxide (NO)in various patho-logical conditions has led to the discovery of new inhib-itors of NO production as potential therapeutic agents.NO,a gaseous free radical,is produced through the oxi-dation of L -arginine by three isoforms of nitric oxide synthase (NOS).1The constitutive NOS (cNOS)found in neuronal tissues (nNOS,type I)and vascular endo-thelium (eNOS,type III)is Ca 2+-dependent and releases small amounts of NO required for homeostatic func-tion.2Meanwhile,inducible NOS (iNOS,type II),which can be induced by lipopolysaccharide (LPS)and various cytokines such as IFN-a ,IL-1b ,and TNF-a ,is Ca 2+-independent and produces micromolar levels of NO.3Low concentrations of NO produced by iNOS possess beneﬁcial roles in antimicrobial activity of macrophages against pathogens,4while the overproduction of NO and its derivatives,such as peroxynitrite and nitrogen diox-ide,has been suggested to be mutagenic in vivo and to provoke the pathogenesis of septic shock and various inﬂammatory processes.5Furthermore,NO and its oxi-dized forms have also been known to be carcinogenic.6Among the many strategies for providing rational con-trol of NO levels,the eﬀorts have been mainly directed toward development of selective inhibitor of iNOS thatcan be applied for the treatment of diseases accompany-ing high levels of NO.Regulation of iNOS can be achieved by the control of expression level and/or enzy-matic activity of iNOS.Many classes of iNOS inhibitors can be classiﬁed according to their structural features,such as,amino acid analogues,7amino heterocycles,8amidines,9guanidines,10isoquinolinamines,11and isot-hiourea.12,13But most of the inhibitors are neither po-tent nor selective enough against NOS isoforms,that limited the application of them in vivo.Only one class of iNOS inhibitors,L -lysine analogue,was reported to enter the clinical trial in human.14Urea was known to inhibit not only the activity of iNOS in macrophages during the uremia,15but also the expres-sion of iNOS in LPS-activated macrophages.16Urea was suggested as an important modulator for renal function through the ﬁne tuning of NO production.17Several groups have reported that urea and thiourea 18or iso-thiourea 12,13,19inhibit iNOS expression and/or NO pro-duction.Based on these reports,we tried to investigate urea and thiourea derivatives as novel inhibitors having mechanism for enzymatic inhibition and/or downregula-tion of iNOS expression.Herein,we report the design and synthesis of urea and thiourea derivatives as depicted in Figure 1.Their eﬀects on the NO production and expression of iNOS were evaluated in LPS-activated macrophage cell culture system.0960-894X/$-see front matter Ó2007Elsevier Ltd.All rights reserved.doi:10.1016/j.bmcl.2007.04.005Keywords :Urea;Thiourea;Carbazole;Nitric oxide synthase;Nitric oxide;Inhibitor.*Corresponding author.Tel.:+8227109571;fax:+8227159571;e-mail:rjeon@sookmyung.ac.krBioorganic &Medicinal Chemistry Letters 17(2007)3317–3321The preparation of the carbazole-linked urea and thio-urea derivatives is outlined in Schemes 1and 2.Hydroxy ethyl group was introduced at nitrogen of carbazole and the resulting alcohol 2was mesylated to obtain com-pound 3.Alkylation of 4-nitrophenol by treatment of compound 3in the presence of NaH gave compound 4.Following reduction of nitro compound 4over 10%Pd/C under atmospheric pressure of hydrogen gas pro-vided amine 5.Condensation of amine 5with the appro-priate isocyanates or isothiocyanates oﬀered the desired compounds 6and 7.The activities of the prepared compounds were evalu-ated for the inhibition of NO production in LPS-acti-vated macrophages.Murine macrophage cell line,RAW 264.7cells,was stimulated with 1l g/mL of LPS in the presence of samples for 20h.The amounts of NO released into culture media were determined by the Griess method 20in the form of nitrite.21The inhibitory activities of the prepared compounds on the NO production are given in Table 1.Aminoguani-dine,a well-known speciﬁc inhibitor of iNOS,was usedas positive control that showed 85%inhibition of NO production at 0.1l M.Most of the thiourea derivatives revealed higher activity than the corresponding urea derivatives.For example,thioureas 7a ,7c ,7e ,and 7f showed signiﬁcantly higher activities than ureas 6a ,6b ,6g ,and 6i ,respectively.Eﬀects of the alkyl substituents at the N1and N3posi-tion of urea 6a and thiourea 7a were investigated.Intro-duction of methyl group 6h at the N1position of urea 6a lowered the activity,while introduction of bulkier sub-stituent retrieved the pound 6i with ethyl group showed the similar activity as 6a .Meanwhile,activity of compound 6j with cyclopropylmethyl at N1became 2.5-fold higher than that of 6a .On the other hand,thiourea derivatives 7f substituted with ethyl and 7g with cyclopropylmethyl at N1of 7a revealed 2-fold lower activity than 7a .The eﬀects of substituents at N3position were consider-ably diﬀerent between urea and thiourea derivatives.While the substitution at N3of urea derivatives 6b–6g showed no signiﬁcant eﬀects on the activity,the alkyl substitution of thiourea greatly enhanced the pounds 7b ,7c ,7e with methyl,ethyl,or carboxym-ethyl group at N3revealed similar activity ranging from 80%to 90%inhibition of NO production at 5l M con-centration.In both cases of ureas and thioureas,the carboxymethyl was the best substituent at N3for the improvement of activity.IC 50values of 6j ,7a–7c ,and3318Y.J.Kim et al./Bioorg.Med.Chem.Lett.17(2007)3317–33217e,which showed more than50%inhibitory responses at 5l M,were determined as3.12,5.31,0.73,0.90,and 0.15l M,respectively.In order toﬁnd the inﬂuence of lipophilic tail on the activity,carbazolylmethyl group of thiourea derivatives 7b–7c was eliminated.These compounds showed lower activities,less than15%inhibition of NO production at5l M,than the corresponding carbazole-linked thiou-reas.It has been reported that a carbazole derivative inhibited iNOS expression in the LPS-activated macro-phage.22Our results also demonstrated that both thio-urea and carbazole moieties might play an important role for their activities although carbazole moiety devoid of thiourea group revealed no activity.We expect to potentiate the activity by the structural modiﬁcation of thiourea and lipophilic segment.For the further biological study of our derivatives,we examined the eﬀects of6j,7a–7c,and7e on the expres-sion of iNOS protein and mRNA in LPS-activated RAW264.7cells.The amounts of iNOS protein were analyzed in Western blot analysis after20-h incubation with compounds during LPS(1l g/mL)activation ofmacrophages.23Compounds7b and7e signiﬁcantly reduced the amounts of iNOS at10l M(Fig.2).At RT-PCR analysis,24the expression level of iNOS mRNA was increased markedly by LPS-activation for pounds7b and7e suppressed the induction of iNOS mRNA at10l M(Fig.3).These results indicated that the inhibition of NO production by thiourea deriv-atives resulted from the suppression of iNOS protein and mRNA.When we treated the compounds after the completion of iNOS induction by LPS(post-treatment),they showed weak activity compared with the results of the co-treat-ment of compounds with LPS.Even the most potent compound7e showed12%inhibition at10l M by post-treatment.These results suggested that thiourea derivatives exhibited their activities mainly through the inhibition of iNOS expression with marginal inhibi-tion against enzymatic activity.It has been reported that urea itself inhibited the activity of iNOS by transcrip-tional15and post-transcriptional16mechanisms.Many of thioureas and isothioureas were reported12,25as inhibitors of iNOS enzyme devoid of controlling the expression step.In addition,a carbazole compound,Table1.Inhibitory activities of carbazole-linked phenylureas and phenylthioureas on the NO production in LPS-induced NO productionNO NHNXR1R2R1, R2=H,alkylX = O, SCompound R1R2X Inhibition a(%)IC50b(nM) 6a H H O246b H Et O306c H Pro O146d H i-Pro O156e H Ph O286f H CH2CO2Et O386g H CH2CO2H O436h Me H O86i Et H O256j Cyclopropylmethyl H O613120±2507a H H S555310±7027b H Me S80730±1807c H Et S90901±2117d H CH2CO2Et S417e H CH2CO2H S87153±837f Et H S337g Cyclopropylmethyl H S34a Values mean the inhibition(%)of NO production at5l M concentration of compounds relative to the LPS control(n=3).b Values are means±SD of three experiments.Y.J.Kim et al./Bioorg.Med.Chem.Lett.17(2007)3317–332133199-(2-chlorobenzyl)-9H-carbazole-3-carbaldehyde,was reported as an inhibitor of iNOS mRNA expression through a signaling pathway that does not involve NF-j B pathway.22The exact diﬀerence between the mecha-nism of our compounds and that of the reported thioureas and carbazole derivative was not explained in this report. The study of the mechanism for the iNOS inhibition by our compounds might be worthy to pursue further.In conclusion,we prepared a series of urea and thiourea derivatives and evaluated their inhibitory activities of NO production in LPS-activated macrophages.They suppressed the release of NO into culture media through the suppression of iNOS protein and mRNA expression. The SAR studies demonstrated that thiourea is superior to urea and N3substitution of thiourea with alkyl group is highly beneﬁcial for their activity.Further study of the other biological activities related with the overproduc-tion of NO,and the detailed mechanism for the activities of these derivatives,is in progress.Our thiourea deriva-tives that can control the expression of iNOS can be good leads for the development of therapeutic agents for the management of NO-related diseases.AcknowledgmentThis work was supported by the SRC program of MOST/KOSEF(R11-2005-017,RESEARCH CEN-TER FOR WOMEN’S DISEASES).Supplementary data Supplementary data associated with this article can be found,in the online version,at doi:10.1016/j.bmcl. 2007.04.005.References and 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dose–response curve was prepared,and the results were typically expressed as IC50values.22.Tsao,L.T.;Lee,C.Y.;Huang,L.J.;Kuo,S.C.;Wang,J.P.Biochem.Pharmacol.2002,63,1961.LPS-+ + + + ++Sample 6j 7a 7b7c 7eFigure3.Eﬀects of the prepared compounds on the expression3320Y.J.Kim et al./Bioorg.Med.Chem.Lett.17(2007)3317–332123.Western blot analysis of iNOS protein expression—RAW264.7cells(1.5·106cells/60-mm dish)were stimulated with LPS(1l g/mL)in the presence or absence of test compounds.After incubation for20h,the cells were washed and lysed with lysis buﬀer.Twenty l g protein of cell lysates was applied on8%SDS–polyacrylamide gels and transferred to PVDF membrane by a standard method.The membrane was probed with antibody for anti-mouse iNOS(Transduction Laboratories,Lexington, KY)and anti-actin(Sigma,St.Louis,MO).The bands were visualized using an enhanced chemiluminescence (ECL)detection kit(Amersham Bioscience,Piscataway, NJ)according to the manufacturer’s instruction.24.Reverse transcription-polymerase chain reaction(RT-PCR)analysis of iNOS mRNA expression—RAW264.7 cells(1.8·106cells/60-mm dish)were stimulated for6h with LPS(1l g/mL)in the presence or absence of test compounds.After washing twice with phosphate-buﬀered saline,total RNA was isolated from cell pellet,using an RNA isolation reagent(Trizol,Invitrogen,Carlsbad,CA).Two micrograms of RNA was reverse transcribed into cDNA using reverse transcriptase and random hexamer.The PCR samples,contained in the reaction mixture,were comprised of mixture buﬀer,dNTP,Taq DNA polymerase (Promega,Madison,WI),and primers(sense and antisense).The sense and antisense primers for iNOS were50-ATGTCCGAAGCAAACATCAC-30and50-TAATGTCCAGGAAGTAGGTG-30,respectively.The sense and antisense primers for b-actin were50-TGT GATGGTGGGAATGGGTCAG-30and50-TTTGATGT CACGCACGATTTCC-30,respectively.The PCR ampli-ﬁcation was performed under following conditions;25 cycles of denaturation at94°C for30s,annealing at55°C for30s,and extension 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