巴西橡胶树乳管细胞Hb WRKY1基因克隆及表达分析

巴西橡胶树乳管细胞Hb WRKY1基因克隆及表达分析
陈月异;杨署光;杨武;田维敏
【期刊名称】《热带作物学报》
【年(卷),期】2011(032)006
【摘要】Laticifer in rubber tree is considered as a defense structure since many defense-related proteins are present there. It is little known about the transcriptional regulation of such proteins. Members of WRKY transcription factor family mediate the responses of plant to biotic and abiotic stresses. In the present study, HbWRKY1 encoding a member of WRKY transcription factor family was cloned from the laticifer of rubber tree clone Reyan7-33-97 by using PCR and RACE techniques. HbWRKYl was 1 755 bp in length and contained an open reading frame of 1 440 bp encoding a putative protein of 479 amino acids. Bioinformatic analysis showed that HbWRKYl contained two WRKY domains followed by a Zinc finger motif of C2H2, respectively, and shared amino acid sequence identity of 64.14%, 60.04%, 42.54%, 40.61% and 35.4% with the members of group I of WRKY transcription factor family from Ricinus communis, [(Populus tomenosa × P. Bolleana) x P. Tomentosa] , Vitis vinifera, Cucumis sativus, and Arabidopsis lhaliana, respectively. Real time quantitative PCR analysis showed that both tapping and ethylene significantly up-regulated the HbWRKYl expression while mechanical wounding and methyl jasmonate had little effect in this respect, suggesting that HbWRKY1 may
mediate the regulation of ethylene signaling on the defense-related proteins in laticifer of rubber tree.%采用PCR和RACE技术,从巴西橡胶树无性系热研7-33-97的乳管细胞中克隆了WRKY转录因子家族的一个成员Hb WRKY1.该基因全长为1 755 bp,含有1个1 440bp阅读框,编码479个氨基酸.生物信息学分析表明,HbWRKY1含有2个WRKY结构域和C2H2锌指结构基序,与蓖麻、杨树、葡萄、黄瓜、拟南芥的WRKY成员的氨基酸序列同源性分别为64.14％、60.04％、42.54％、40.61％和35.4％,属于Ⅰ类WRKY家族成员.实时荧光定量PCR分析表明,割胶和乙烯显著上调Hb WRKY1基因的表达,但茉莉酸和机械伤害对HbWRKY1的表达没有明显影响,表明HbWRKY1可能在乙烯信号途径对防卫蛋白的调节中起重要作用.
【总页数】6页(P987-992)
【作者】陈月异;杨署光;杨武;田维敏
【作者单位】海南大学园艺园林学院,海南儋州 571737;中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室海南儋州571737;中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室海南儋州571737;海南大学农学院,海南儋州571737;中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室海南儋州571737
【正文语种】中文
【中图分类】S794.1
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