临床症状不同颈椎病患者颈椎间盘白细胞介素1β及白细胞介素6和环氧化酶2的表达

临床症状不同颈椎病患者颈椎间盘白细胞介素1β及白细胞介
素6和环氧化酶2的表达
胡炜;马信龙;袁建军;张仁赞;彭兵;张学利
【期刊名称】《中国组织工程研究》
【年(卷),期】2016(020)035
【摘要】背景：在颈椎的退变过程中，椎间盘细胞发生生物化学变化，此过程产
生各种炎性细胞因子可能导致椎间盘突出。

间盘突出后，反过来刺激周围毗邻组织产生各种炎性因子。

<br> 目的：探讨白细胞介素1β、白细胞介素6、环氧化酶2在不同临床症状的颈脊髓压迫患者中的表达及意义。

方法：颈前路间盘切除
内固定患者的突出间盘或责任间盘，依据临床症状分为3组：髓型症状组，根性
症状组，颈椎外伤组，对间盘进行苏木精-伊红染色及免疫组织化学染色进行形态
学观察，对间盘免疫组织化学染色结果进行阳性细胞计数。

<br> 结果与结论：①髓型症状组和根性症状组苏木精-伊红染色在突出颈椎间盘周围的炎性肉芽组织
中可见炎细胞浸润以及新生血管形成；颈椎外伤组未见明显炎细胞浸润以及新生血管形成；②免疫组织化学染色髓型症状组可见白细胞介素1β、白细胞介素6表达阳性的细胞，其胞浆为棕黄色着色为主，环氧化酶2表达阳性的细胞稀少；根性
症状组中可见白细胞介素1β、白细胞介素6、环氧化酶2表达阳性的细胞，数量
明显多于髓型症状组；颈椎外伤组中免疫组织化学染色白细胞介素1β、白细胞介
素6、环氧化酶2表达阳性细胞表达稀少；③髓型症状组及根性症状组中白细胞介素1β阳性表达率和IA值，白细胞介素6阳性表达率和IA值均显著高于颈椎外伤组(P<0.05)；根性症状组中环氧化酶2阳性表达率及IA值均显著高于颈椎外伤组。

④结果说明，突出的颈椎间盘可发生白细胞介素1、白细胞介素6、环氧化酶2细
胞因子表达，并在颈椎间盘早期退变中发挥作用，不同临床症状的颈脊髓压迫患者这些炎症因子表达具有明显差异。

%BACKGROUND:During degeneration of cervical spine, biochemical changes appeared in intervertebral disc cel s. During this process, a variety of inflammatory cytokines may lead to disc herniation, which stimulates the production of a variety of inflammatory factors from surrounding adjacent tissue. <br> OBJECTIVE:To explore the expression and significance of interleukin-1β, interleukin-6 and cyclooxygenase 2 in patients with different clinical symptoms of cervical spinal cord oppression. <br> METHODS:Protrusion of the intervertebral disc or disc of responsibility among patients with anterior disc resection and internal fixation were divided into three groups according to clinical symptoms:myelopathic symptom group, nerve root symptom group and cervical spine trauma group. Intervertebral disc received hematoxylin-eosin staining and immunohistochemical staining for morphological observation. Positive cel s were counted according to the result of immunohistochemical staining. <br> RESULTS AND CONCLUSION:(1) Hematoxylin-eosin staining results showed visible inflammatory cel infiltration and new blood vessel formation in the myelopathic symptom group and nerve root symptom group. No remarkable inflammatory cel infiltration or new blood vessel formation was seen in cervical spine trauma group. (2) Immunohistochemical staining interleukin-1β-, interleukin-6-positive cel s were seen in the myelopathic symptom group. Cytoplasm was stained tan. Cyclooxygenase 2-positive cel s showed a low number. The numbers of interleukin-1β-, interleukin-6-and cyclooxygenase
2-positive cel s were significantly more in the nerve root symptom group than in the myelopathic symptom group. The numbers of interleukin-1β-, interleukin-6-and cyclooxygenase 2-positive cel s were smal in the cervical spine trauma group. (3) Expression rate and IA value of interleukin-1β, expression rate and IA value of interleukin-6 were significantly higher in the myelopathic symptom group and nerve root symptom group than in the cervical spine trauma group (P<0.05). Expression rate and IA value of cyclooxygenase 2 were significantly higher in the nerve root symptom group than in the cervical spine trauma group. (4) These results suggested that interleukin-1β, interleukin-6 and cyclooxygenase 2 expression could be found in the cervical intervertebral disc after protrusion, and played a role in early degeneration of cervical intervertebral disc. The expressions of these inflammatory factors were significantly different in patients with different clinical symptoms of cervical spinal cord compression.
【总页数】7页(P5270-5276)
【作者】胡炜;马信龙;袁建军;张仁赞;彭兵;张学利
【作者单位】天津市人民医院脊柱外科，天津市 300121;天津医院骨科，天津市300210;天津市人民医院脊柱外科，天津市 300121;天津市人民医院脊柱外科，天津市 300121;天津市人民医院脊柱外科，天津市 300121;天津市人民医院脊柱外科，天津市 300121
【正文语种】中文
【中图分类】R318
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