高效液相色谱法同时测定牛肉组织中6种类固醇类激素类药物

高效液相色谱法同时测定牛肉组织中6种类固醇类激素类药
物
衣闻闻;全灿;金君素
【期刊名称】《化学分析计量》
【年(卷),期】2011(20)3
【摘要】A HPLC method was established for the simultaneous determination of six anabolic - androgenic steroids including methandienone, nandrolone, boldenone, methyltestosterone, testosterone propionate and nantrolone propionate in beef muscle tissues. The samples were extracted with acetonitrile and then cleaned up by freezing - lipid filtration. The mobile phase consisted of methanol - water at flow rate of 1.0 Ml/min. The detection limits for the method were 0.0051-0.008 6
mg/kg, and the limits of quantification were 0. 0220 -0.0287 mg/kg. The linear calibration curves were obtained in the concentration range of 0. 25 - 10 mg/L, with r2 more than 0.999. The recoveries of the six compounds were 84. 1% - 100. 0% fortified at the 0.4 mg/kg and 2. 0 mg/kg levels, and the relative standard deviations of determination results were 0.5% -
3.5%(n=5). The established method is simple, rapid, sensitive and specific, and it is appropriate for the identification and quantification of anabolic androgenic steroids in animal muscle tissues.%建立了高效液相色谱法同时测定牛肉组织中勃地龙、诺龙、美雄酮、甲基睾酮、丙酸睾酮和丙酸诺龙的分析方法.试样经乙腈提取,冷冻离心脱脂净化,以甲醇-水为流动相,梯度洗脱,流速为1.0
mL/min.该方法的检出限(LOD)为0.0051～0.0086 mg/kg,定量限(LOQ)为
0.0220～0.0287 mg/kg,在0.25～10 mg/L的线性范围内,相关系数r2均大于0.999.在0.4 mg/kg和2.0 mg/kg的添加水平上,上述6种激素的平均回收率为84.1%～100.0%,测定结果的相对标准偏差为0.5%～3.5%(n=5).该方法的样品前处理简单、快速、稳定性好,可用于牛肉组织中上述6种蛋白同化类激素的同时测定.
【总页数】4页(P26-29)
【作者】衣闻闻;全灿;金君素
【作者单位】北京化工大学化学工程学院,北京100029;中国计量科学研究院化学计量与分析科学研究所,北京100013;北京化工大学化学工程学院,北京100029【正文语种】中文
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