牛磺酸对H2O2引起的大鼠血管平滑肌细胞核［Ca2+］rn及胞浆［Ca2+］变化的影响

牛磺酸对H2O2引起的大鼠血管平滑肌细胞核［Ca2+］rn
及胞浆［Ca2+］变化的影响
郝胜利;张孙曦;唐朝枢;何其华;凤志慧;安丽芝
【期刊名称】《中国药理学通报》
【年(卷),期】2001(017)001
【摘要】AIM As one of the reactive oxygen species, toxic dose of H2O2 leads to the necrosis or apoptosis of many kinds of cells. It is nuclear whether these processes are related with the changes of nuclear Ca2+ elicited by H2O2. This study on the effect of taurine on changes of nuclear and cytosolic (Ca2+) elicited by H2O2 in rat vascular smooth muscle cells. METHODS The techniques of Fluo-3 and laser scanning confocal microscopy were used. RESULTS Rapid and sustaining rise of nuclear ［Ca2+］ (［Ca2+］n) and cytosolic ［Ca2+］(［Ca2+］c) were found in cultured rat vascular smooth muscle cells upon adding of 0.5% H2O2. Pretreatment of the cells with 20 mmol*L-1 taurine, a regulator of cellular Ca2+ homestasis, significantly decreased the rise amplitude of ［Ca2+］n (P<0.05), but failed to affect that of ［Ca2+］c (P>0.05) elicited by H2O2. It was suggested that the changes of ［Ca2+］n and ［Ca2+］c have different respond to taurine. CONCLUSION It was concluded that there exist the independent regulating mechanisms of Ca2+ in the nucleus from differences between the nucleus and cytosol Ca2+ in sensitivity to action of H2O2 and taurine.%目的毒性剂量H2O2可引起细胞坏死或凋亡, 此过程是否
与H2O2引起的核［Ca2+］（［Ca2+］n）变化有关未见报导。

本文研究牛磺酸对H2O2引起的血管平滑肌细胞（VSMC）［Ca2+］n与胞浆［Ca2+］
(［Ca2+］c)的变化影响。

方法应用激光共聚焦显微镜对负载Fluo-3的培养大鼠VSMC的［Ca2+］n及［Ca2+］c变化进行研究。

结果在0.5% H2O2作用下，［Ca2+］n与［Ca2+］c持续升高，用抗氧化细胞保护剂-牛磺酸20 mmol*L-1预处理细胞，可降低H2O2引起的［Ca2+］n 升幅(P<0.05)，但不影响［Ca2+］c升幅(P>0.05)，表明［Ca2+］n变化与［Ca2+］c变化对牛磺酸反应性存在差异。

结论 VSMC的核Ca2+调控与胞浆Ca2+调控各自具有一定的独立性。

牛磺
酸对H2O2引起的大鼠VSMC核Ca2+的变化具有保护作用。

【总页数】3页(P89-91)
【作者】郝胜利;张孙曦;唐朝枢;何其华;凤志慧;安丽芝
【作者单位】北京医科大学生物物理系,;北京医科大学生物物理系,;北京医科大学生物物理系,;北京医科大学生物物理系,;北京医科大学生物物理系,;北京医科大学生物
物理系,
【正文语种】中文
【中图分类】R-332;R322.74;R348.2;R329.26;R914.3;R914.4
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