脂肪的秘密认识肥胖与健康1
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Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Background What is the human FAP markers in human muscle?
Outline
Backgroud Results Questions and thinking
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
The relationship between FAP and diebetes
Results
Human skeletal muscle FAPs can be prospectively isolated as CD34+CD56–CD45–CD31–
Results
FAPs (FAP CD90- and FAP CD90+ combined) constitute a major proportion of the mononuclear (~40%) cells in our patient-derived human skeletal muscle.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
1. Acute Hypoglycemia in Healthy Humans Impairs Insulin-Stimulated Glucose Uptake and Glycogen
Synthase in Skeletal Muscle: A Randomized Clinical Study. Diabetes. 2017 Sep;66(9):2483-2494. 2 Gormsen, Lars C; Sondergaard, Esben; Christensen, Nana L; Brosen, Kim; Jessen, Niels; Nielsen, Soren Metformin increases endogenous glucose production in non-diabeticindividuals and individuals with recent-onset type 2 diabetes. Diabetologia, Vol. 62, 07.2019, p. 1251–1256. 3. Niels Jessen, Esben Selmer Buhl, Ole Schmitz, Sten Lund.Impaired insulin action despite upregulation of proximal insulin signaling: novel insights into skeletal muscle insulin resistance in liver cirrhosis. Journal of Hepatology,2006 Dec;45(6):797-804.
The relationship between FAP and diebetes
Results
DE genes were enriched in biological processes and pathways related to ECM turnover and remodeling in itT2D patients.
Results
These also revealed an increased deposition of Collagen1 in T2DM patients, as compared to patients without T2DM.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
The relationship between FAP and diebetes
Results
Human Single-cell RNA sequencing confirms the identity of FAPs.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
Outline
Backgroud Results Qeustions and thinking
Backgroundபைடு நூலகம்
Skeletal muscle is a important organ for overall body healthy comprising 40% of human body mass.
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Corresponding author
Niels Jessen
Head of Research, Professor in clinical pharmacology Department of Biomedicine, Aarhus University，Denmark
Clinical Diebetes
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Results
There are 10 times stem cell frequency in MuSCs the FAPs .
Results
Results
Human FAPs are mitotically inactivein vivo(as MuSCs), and upon acti-vation they display a slow kinetic of progression into S-phase,compared to MuSCs.
The paper demonstrates that degeneration of skeletal muscle in individuals with type 2 Diabetes is associated with the accumulation of a subset of fibroadipogenic progenitors, expressing CD90, endowed with enhanced progenitor potential and collagen production.
The relationship between FAP and diebetes
Results
PDGFRa expression was associated with COL1A1 expression in FAPs
Background
In mice, skeletal muscle resident cells with fibro- and adipogenic potential (i.e., FAPs) were identified a decade ago by two independent reports as stem cell antigen 1+, CD34+, platelet-derived growth factor receptora(PDGFRa)+cells
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Background
Degeneration of skeletal muscle are associated with a multitude of different diseases and ultimately shortened lifespan
Background
Muscle cells are progressively replaced by non-contractile tissue including extracellular matrix (ECM) proteins and adipocytes under the circumstances of
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
skeletal muscle degeneration
Background
T2DM are also associated with fibro-fatty degeneration human skeletal muscle.
Background
What is the cellular origin of fibrosis and adipocytes ?
The relationship between FAP and diebetes
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Background What is the human FAP markers in human muscle?
Outline
Backgroud Results Questions and thinking
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
The relationship between FAP and diebetes
Results
Human skeletal muscle FAPs can be prospectively isolated as CD34+CD56–CD45–CD31–
Results
FAPs (FAP CD90- and FAP CD90+ combined) constitute a major proportion of the mononuclear (~40%) cells in our patient-derived human skeletal muscle.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
1. Acute Hypoglycemia in Healthy Humans Impairs Insulin-Stimulated Glucose Uptake and Glycogen
Synthase in Skeletal Muscle: A Randomized Clinical Study. Diabetes. 2017 Sep;66(9):2483-2494. 2 Gormsen, Lars C; Sondergaard, Esben; Christensen, Nana L; Brosen, Kim; Jessen, Niels; Nielsen, Soren Metformin increases endogenous glucose production in non-diabeticindividuals and individuals with recent-onset type 2 diabetes. Diabetologia, Vol. 62, 07.2019, p. 1251–1256. 3. Niels Jessen, Esben Selmer Buhl, Ole Schmitz, Sten Lund.Impaired insulin action despite upregulation of proximal insulin signaling: novel insights into skeletal muscle insulin resistance in liver cirrhosis. Journal of Hepatology,2006 Dec;45(6):797-804.
The relationship between FAP and diebetes
Results
DE genes were enriched in biological processes and pathways related to ECM turnover and remodeling in itT2D patients.
Results
These also revealed an increased deposition of Collagen1 in T2DM patients, as compared to patients without T2DM.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
The relationship between FAP and diebetes
Results
Human Single-cell RNA sequencing confirms the identity of FAPs.
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance
Outline
Backgroud Results Qeustions and thinking
Backgroundபைடு நூலகம்
Skeletal muscle is a important organ for overall body healthy comprising 40% of human body mass.
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Corresponding author
Niels Jessen
Head of Research, Professor in clinical pharmacology Department of Biomedicine, Aarhus University，Denmark
Clinical Diebetes
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Results
There are 10 times stem cell frequency in MuSCs the FAPs .
Results
Results
Human FAPs are mitotically inactivein vivo(as MuSCs), and upon acti-vation they display a slow kinetic of progression into S-phase,compared to MuSCs.
The paper demonstrates that degeneration of skeletal muscle in individuals with type 2 Diabetes is associated with the accumulation of a subset of fibroadipogenic progenitors, expressing CD90, endowed with enhanced progenitor potential and collagen production.
The relationship between FAP and diebetes
Results
PDGFRa expression was associated with COL1A1 expression in FAPs
Background
In mice, skeletal muscle resident cells with fibro- and adipogenic potential (i.e., FAPs) were identified a decade ago by two independent reports as stem cell antigen 1+, CD34+, platelet-derived growth factor receptora(PDGFRa)+cells
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
Background
Degeneration of skeletal muscle are associated with a multitude of different diseases and ultimately shortened lifespan
Background
Muscle cells are progressively replaced by non-contractile tissue including extracellular matrix (ECM) proteins and adipocytes under the circumstances of
Isolated human skeletal muscle FAPs as CD34+CD56–CD45–CD31– Single-cell RNA sequencing confirms the identity of FAPs
The Characteristics of FAPs
The relationship between subpopulation of FAPs
skeletal muscle degeneration
Background
T2DM are also associated with fibro-fatty degeneration human skeletal muscle.
Background
What is the cellular origin of fibrosis and adipocytes ?
The relationship between FAP and diebetes
Results
RNA-Seq in muscle biopsies from age- and overweight-matched subjects (OBS), T2DM patients (T2D), and insulin-treated T2DM patients (itT2D) with severe insulin resistance