雷公藤多苷含药血清对强直性脊柱炎病理性骨化相关炎症因子和miR-21的影响

雷公藤多苷含药血清对强直性脊柱炎病理性骨化相关炎症因子
和miR-21的影响
邹宇聪;毛筝;徐敏鹏;胡少俊;接红宇;陈俊琦;刘刚
【期刊名称】《实用医学杂志》
【年(卷),期】2017(33)3
【摘要】目的:探讨雷公藤多苷含药血清对强直性脊柱炎(AS)病理性骨化相关炎症因子及miR-21的影响.方法:将本题组前期培养分离的AS滑膜成纤维细胞用l
ng/mL IL-1刺激24h后,加入不同浓度的空白血清(5％,10％,15％)或雷公藤多苷含药血清(5％,10％,15％)培养48 h,Western Blot检测PGE-2、IL-17、IL-22、IL-23、CCL19、CCL21的水平,RT-PCR检测成骨相关因子BMP-2以及miR-21的表达.结果:雷公藤多苷含药血清在干预后48 h,显著抑制了炎症因子的表达,并同时抑制了BMP-2和miR-21的表达,且这一抑制呈浓度梯度改变.结论:雷公藤多苷含药血清可以抑制AS病理性骨化相关炎症因子,BMP-2和miR-21的表达,为其治疗AS病理性骨化提供了理论基础.%Objective To investigate the effect of tripterygium glycosides (TG) contained serurn on the pathological boneforming related inflammatory markers and miR-21.Methods Previous isolated and cultured AS fibroblasts were stimulated using IL-1 of 1ng/ml for 24h,different concentrations of blank serum (5％,10％,and 15％) and TG contained serum (5％,10％,and 15％) were added for 48h.PGE-2,IL-17,IL-22,IL-23,CCL19 and CCL21 proteins were examined by Western blot.The osteogenesis marker BMP and microRNA-21 mRNAs were tested.Results 48 h after intervention,the expressions of inflammatory markers were
obviously inhibited by TG contained serum;the boncforming related inflammatory markers,expressions of BMP-2 and miR-2t were all inhibited in a dose-dependent manner.Conclusions TG could inhibit the expressions of boneforming related inflammatory markers,BMP-2 and miR-21,thus providing theoretical basis to treat AS pathological boneforming.
【总页数】4页(P367-370)
【作者】邹宇聪;毛筝;徐敏鹏;胡少俊;接红宇;陈俊琦;刘刚
【作者单位】510630广州市,南方医科大学第三附属医院康复科;510630广州市,南方医科大学第三附属医院康复科;510630广州市,南方医科大学第三附属医院康复科;510630广州市,南方医科大学第三附属医院康复科;510630广州市,南方医科大学第三附属医院风湿科;510630广州市,南方医科大学第三附属医院康复
科;510630广州市,南方医科大学第三附属医院康复科
【正文语种】中文
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