川芎嗪改善脑缺血后学习记忆障碍的实验研究

川芎嗪改善脑缺血后学习记忆障碍的实验研究
姚国恩;王景周;陈曼娥
【期刊名称】《中国组织工程研究》
【年(卷),期】2003(007)013
【摘要】Aim To evaluate the therapeutic effect of active avoidance response on impairment of learning and memory ability after cerebral ischemic injury.Methods 90 Wistar rats were randomly divided into three groups:the control group,the brain ischemic group and TMP-treated group.Each group was observed at 3 time points:the 2nd week,the 4th week and the 2nd month after ischemia.The VD rat model was established by four-vessel occlusion(4VO).The changes of memory ability, LTP and the NMDAR1mRNA expression in the hippocampus were administered by computerized shuttle-training case, extracorporeal hippocampal slices method and in situ hybridization.TMP was injected into abdominal cavity within 5 minutes after the cerebral reperfution.Results Compared with the control group (2nd week:90%± 5% ;4th week:92%± 6% ;2nd month:91%± 6% ),the AAR in the brain ischemic group was significantly lower in the 2nd week(60%± 5% ), the 4th week (40%± 8% ) and the 2nd month (41%± 8% ) (t=1.852,P< 0.05),and it was more obviously at the later two time
points(t=2.947,3.504,P< 0.01). In comparison with the brain ischemic group, the AAR of TMP-treated group was absolutely
improved(t=1.803,P< 0.05).In the slices of extracorporeal hippocampal CA1
region,LTP detection showed that its injury had happened clearly in brain ischemic group at each time point of the 2nd week,the 4th week and the 2nd month.LTP wave could induce significantly in CO group and TMP group;LTP wave could hardly induce at each time point in BI
paring the percentage of fEPSP slope changes before and after conditioned stimulation,there was a significant difference between CO group and TMP group(t=4.064,3.655,P< 0.01),but no significant difference among each time points; at the 4th week and the 2nd month,it was decreased in each pared with brain ischemic group, significant changes were found in the TMP-treated group at each time
point.Conclusion In the early phase of ischemic re-perfusion, TMP can improve impairment of learning and memory.%目的通过利和大鼠模型对中药川芎嗪对脑缺血后认知障碍的疗效进行评价,探讨其可能的机制.方法选用主动回避反应( AAR)≥ 80%的健康老龄 Wistar大鼠 90只进行 7 d穿梭箱训练后以随机数字表法分为对照组、脑缺血组、川芎嗪组.用改良 Pulsinelli's四血管阻断( 4VO)法建立血管性痴呆大鼠模型;采用电脑控制的穿梭箱系统和海马脑片诱导的 CA1区长时程增强( LTP)检测大鼠学习记忆功能;原位杂交方法检测大鼠海马
NMDAR1mRNA的表达;在大鼠 4VO后 5 min内腹腔内注射川芎嗪,观察上述指标变化.结果脑缺血( BI)组大鼠的 AAR在 2周时为( 60± 5)%较对照( CO)组90%± 5%有所下降( t=1.852,P< 0.05),脑缺血( BI)组大鼠的 AAR在 4周和 2个月时分别为40%± 8%, 41%± 8%较 CO组92%± 6%, 91%± 6%有所下降更加明显( t=2.947,3.504,P< 0.01);川芎嗪( TMP)组各时相点 AAR与 BI组比较均有明显改善( t=1.803,P< 0.05). CO组和 TMP组均可明显诱出 LTP波形; BI组各时相点均几乎诱不出 LTP,条件刺激前后场兴奋性突触后电位( fEPSP)斜率变化的百分数与
CO组和 TMP组比较差异有显著性意义( t=4.064,3.655,P< 0.01),但各时相点间差异无显著性意义. BI组 2周时 CA1和 CA3区 N-甲基- D-天冬氨酸受体
1( NMDAR1) mRNA表达较正常对照组增高,但 4周和 2个月时表达减低; TMP 组与 BI组比较有明显差别.结论在缺血性脑血管病缺血再灌注的早期,应用 TMP可以改善脑缺血后学习记忆障碍.
【总页数】3页(P1877-1879)
【作者】姚国恩;王景周;陈曼娥
【作者单位】解放军第三军医大学大坪医院神经内科,重庆市,400042;解放军第三军医大学大坪医院神经内科,重庆市,400042;解放军第三军医大学大坪医院神经内科,重庆市,400042
【正文语种】中文
【中图分类】R743.3
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