酶切Buffer图谱

NR 20-50 0-20 50-100
(+SAM)
100 20-50
NR 0-20 20-50 0-20 0-20 0-20 20-50 100 20-50 0-20 50-100 0-20 20-50 0-20 20-50 20-50 20-50 20-50 0-20
(+oligo)
20-50 50-100*
Unique
Ecl136II (EcoICRI) GAG↓CTC
Unique
Eco24I (BanII)
GRGCY↓C
Tango™
Eco31I (BsaI)
GGTCTC(1/5)↓
G
Eco32I (EcoRV)
GAT↓ATC
R
Eco47I (AvaII)
G↓GWCC
R
Eco47III (AfeI)
AGC↓GCT
C↓YCGRG
Tango™
Eco91I (BstEII)
G↓GTNACC
O
Eco105I (SnaBI) TAC↓GTA
Tango™
Eco130I (StyI)
C↓CWWGG
O
Eco147I (StuI)
AGG↓CCT
B
EcoO109I (DraII) RG↓GNCCY
Tango™
EcoRI
G↓AATTC
NR 20-50 100 50-100
(+SAM)
0-20 20-50
NR 100 0-20 50-100 50-100 50-100 0-20 20-50 0-20 20-50 20-50 100 0-20 20-50 100 50-100 20-50 100 20-50
(+oligo)
50-100 0-20 0-20 50-100* 20-50 0-20 20-50 100 0-20 50-100 20-50 0-20 0-20 0-20 20-50 0-20 100 100 100 20-50 20-50
(+oligo)
20-50 50-100 0-20 20-50 50-100 50-100 50-100 50-100* 50-100
100 50-100 50-100 50-100 20-50 50-100
100 50-100 50-100 20-50 0-20
100
(+SAM)
50-100
(+SAM)
GACNN↓NNGTC
Tango™
BpiI (BbsI)
GAAGAC(2/6)↓
G
BplI
↓(
8
/13
)
G
AG
(
N
)
CTC
5
(13
/8
)↓
Tango™
(+SAM)
Bpu10I
CCTNAGC(-5/-2)↓
Unique
Bpu1102I (BlpI) GC↓TNAGC
Tango™
BseDI (BsaJI) (55°C) C↓CNNGG
R
BseMII (BspCNI)neo CTCAG(10/8)↓
(55°C)
Tango™
(+SAM)
BseNI (BsrI) (65°C) ACTGG(1/-1)↓
B
BseSI (Bme1580I) (55°C) GKGCM↓C
G
BseXI (BbvI) (65°C) GCAGC(8/12)↓
Unique
Recom-
Enzyme
Specificity 5’→3’
mended buffer for 100% activity
AanI (PsiI)
TTA↓TAA
Tango™
AarI
CACCTGC(4/8)↓
Unique
(+oligo)
AasI (DrdI)
GACNNNN↓NNGTC
B
AatII
GACGT↓C
(+DTT)
20-50
(+SAM)
0-20 50-100
50-100
NR
20-50 20-50 20-50 100
NR 50-100
(+SAM)
0-20
(+SAM)
0-20 0-20 20-50 100 100 20-50 100 50-100 100 50-100 50-100 100
(+SAM)
0-20
(+SAM)
0-20 50-100 0-20 50-100 50-100
100 20-50* 0-20 20-50 20-50 50-100
NR
20-50 0-20 NR 0-20 0-20 20-50 0-20 20-50 0-20
(+SAM)
0-20 50-100 50-100 20-50
O
Eco52I (EagI)
C↓GGCCG
Unique
Eco57I (AcuI)
CTGAAG(16/14)↓
G
(+SAM)
Eco57MI
CTGRAG(16/14)↓
B
(+SAM)
Eco72I (PmlI)
CAC↓GTG
Tango™
Eco81I (Bsu36I) CC↓TNAGG
Tango™
Eco88I (AvaI)
CG↓GWCCG
Tango™
CseI (HgaI)
GACGC(5/10)↓
R
Csp6I (CviQI)
G↓TAC
B
DpnI
Gm6A↓TC
Tango™
DraI
TTT↓AAA
Tango™
Eam1104I (EarI) CTCTTC(1/4)↓
Tango™
Eam1105I (AhdI) GACNNN↓NNGTC
(+SAM)
0-20
(+SAM)
0-20 0-20 0-20 100 0-20 100 20-50 20-50 100 100 0-20 0-20
(+DTT)
0-20
(+SAM)
100 0-20
0-20 0-20
(+oligo)
0-20 0-20 20-50 100 NR 100
(+SAM)
100
(+SAM)
Fermentas Conventional Restriction Enzymes Buffer Activity Chart
/doubledigest /research /reviewer
Tango™
Enzyme activity in Fermentas buffers, %
B (blue) G (green) O (orange) R (red)
1X
1X
1X
1X
Tango™ (yellow)
1X
2X
50-100
NR
100 50-100 0-20 0-20
NR 0-20
(+SAM)
Tango™
Acc65I (KpnI)
G↓GTACC
O
AdeI (DraIII)
CACNNN↓GTG
G
AjiI (BmgBI)
CAC↓GTC
Unique
AjuI
↓(
7/12)
G
A
A(
N
)T
7
TGG
(11/
6
)
↓
R
(+SAM)
AlfI
↓(10 /12 ) GCA ( N ) 6TGC (12 /10 )↓
ApaI
GGGCC↓C
B
BamHI
G↓GATCC
Unique
BauI (BssSI)
CACGAG(-5/-1)↓
Tango™
BclI (55°C)
T↓GATCA
G
BcnI (NciI)
CC↓SGG
Tango™
BcuI (SpeI)
A↓CTAGT
Tango™
BdaI (30°C)
↓(10/12)TGA(N) TCA(12/10)↓ 6
Tango™
BseGI (FokI)neo (55°C) GGATG(2/0)↓
Tango™
BseJI (BsaBI) (65°C) GATNN↓NNATC
O
BseLI (BslI) (55°C) CCNNNNN↓NNGG
Tango™
BseMI (BsrDI) (55°C) GCAATG(2/0)↓
NR 100 50-100 20-50 50-100 20-50 50-100 20-50 NR 50-100 50-100 20-50
(+DTT)
20-50
(+SAM)
0-20 20-50
0-200 20-50 20-50* 20-50
(+SAM)
C↓TTAAG
O
Bst1107I (BstZ17I) GTA↓TAC
O
BstXI (55°C)
CCANNNNN↓NTGG
O
Bsu15I (ClaI)
AT↓CGAT
Tango™
BsuRI (HaeIII)
GG↓CC
R
BveI (BspMI)
ACCTGC(4/8)↓
O
(+oligo)
CaiI (AlwNI)
B
Bsp143I (Sau3AI) ↓GATC
Unique
Bsp1407I (BsrGI) T↓GTACA
Tango™
BspLI (NlaIV)
GGN↓NCC
Tango™
BspOI (BmtI)
GCTAG↓C
O
BspPI (AlwI) (55°C) GGATC(4/5)↓
Tango™
BspTI (AfIII)
Unique
EcoRII
↓CCWGG
O
EheI (NarI)neo
GGC↓GCC
Tango™
Esp3I (BsmBI)
CGTCTC(1/5)↓
Tango™
(+DTT)
FaqI (BsmFI)
GGGAC(10/14)↓
Tango™
(+SAM)
FspAI
RTGC↓GCAY
O
FspBI (BfaI)
C↓TAG
0-20 0-20 50-100 100 20-50 NR 100 100 50-100 50-100 20-50 50-100 50-100 50-100 0-20 0-20 0-20 0-20 100
(+SAM)
100
(+SAM)
NR 50-100
100 20-50 100* 0-20 100 50-100 0-20 20-50 20-50 100
20-50 50-100
NR 50-100 20-50 50-100 0-20
100 20-50
(+SAM)
20-50* 50-100 20-50 50-100 100*
100 20-50 50-100
(+SAM)
20-50 100 NR 0-20 100 20-50 20-50 20-50 0-20 20-50 20-50 20-50 50-100 0-20 20-50 0-20 50-100 100 20-50 20-50 20-50
(+SAM)
0-20 0-20 NR 50-100 20-50 100 100 100 0-20 0-20 0-20 0-20 0-20 100 0-20 100 100 100 20-50 50-100 100
(+oligo)
20-50 0-20 0-20 20-50 20-50 0-20 50-100 50-100 0-20 50-100 20-50 0-20 0-20 0-20 0-20 0-20 50-100 50-100 100 0-20 20-50
R
(+SAM)
AloI (30°C) AluI
↓(7/12-13)GAAC(N) TCC(12-13/7)↓ 6
R
AG↓CT
Tango™
Alw21I (BsiHKAI) GWGCW↓C
O
Alw26I (BsmAI)
GTCTC(1/5)↓
Tango™
Alw44I (ApaLI)
G↓TGCAC
Tango™
0-20
(+SAM)
0-20 0-20 100 0-20 0-20 0-20 20-50 0-20 20-50 50-100 0-20 0-20
(+SAM)
0-20 0-20 0-20 100 100 100 0-20 50-100 0-20
(+SAM)
50-100* 20-50 0-20 20-50 100 50-100 0-20 50-100
Bsh1236I (BstUI) CG↓CG
R
Bsh1285I (BsiEI) CGRY↓CG
G
BshNI (BanI)
G↓GYRCC
O
BshTI (AgeI)
A↓CCGGT
O
Bsp68I (NruI)
TCG↓CGA
O
Bsp119I (BstBI)
TT↓CGAA
Tango™
Bsp120I (ApaI)neo G↓GGCCC
CAGNNN↓CTG
Tango™
CfrI (EaeI)
Y↓GGCCR
Tango™
Cfr9I (XmaI)
C↓CCGGG
Unique
Cfr10I (BsrFI)
R↓CCGGY
Unique
Cfr13I (Sau96I)
G↓GNCC
Tango™
Cfr42I (SacII)
CCGC↓GG
B
CpoI (RsrII)
100 0-20 50-100 0-20 50-100 0-20 50-100* 50-100 20-50 50-100 20-50 20-50
(+SAM)
0-20 0-20 0-20 100 50-100 50-100 20-50 50-100 0-20
(+SAM)
100* 20-50 0-20 20-50
G
(+SAM)
BfiI (BmrI)
ACTGGG(5/4)↓
Tango™
BfmI (SfcI)
C↓TRYAG
Tango™
BfuI (BciVI)
GTATCC(6/5)↓
Unique
BglI
GCCNNNN↓NGGC
O
BglII
A↓GATCT
O
Bme1390I (ScrFI) CC↓NGG
O
BoxI (PshAI)