仙人掌多糖对大鼠局灶性脑缺血的神经保护作用

仙人掌多糖对大鼠局灶性脑缺血的神经保护作用
唐焜;谢小慧;陈志达;陈扬;郭莲军
【期刊名称】《医药导报》
【年(卷),期】2012(31)9
【摘要】目的观察仙人掌多糖对大脑中动脉栓塞(MCAO)诱导的大鼠缺血-再灌注损伤的神经保护作用.方法雄性SD大鼠随机分为假手术组、缺血组、0.9％氯化钠溶液组、仙人掌多糖治疗组(200 mg·kg-1·d-1,ip).采用MCAO法制作大鼠缺血-再灌注损伤模型.分别于大鼠缺血2h再灌注3,6,8h后进行神经行为学评分;8h后2,3,5-三苯基氯化四氯唑(TTC)染色测定脑梗死体积;缺血2h再灌注22 h后脑组织切片苏木精-伊红(HE)染色显微镜下形态学观察.结果与0.9％氯化钠溶液组相比,再灌注8h后仙人掌多糖治疗组神经行为学评分平均下降(2.35±0.47)分(P＜0.05),梗死灶体积减少(P＜0.05);大鼠皮质及海马组织神经细胞丢失、神经胶质增生、核固缩、核深染等形态学均有明显改善(P＜0.05).结论仙人掌多糖可以缓解大鼠大脑中动脉栓塞症状,具有一定的神经保护作用.%Objective To study the neuroprotective effect of cactus polysaccharides (CP) on
ischemia/reperfusion(I/ R) injury induced by the middle cerebral artery occlusion ( MCAO) in rats. Methods The male SD rats were randomly divided into sham-operated, MCAO/R model group, NS-treated group,CP- treated group (200 mg · kg-1 · d-1 ,ip).The MCAO/ R model was made by MCAO method. The neurological deficits were detected at 3 h,6 h and 8 h after MCAO for 2 h and reperfusion; The cerebral infarct volume was detected by TTC staining 8 h later, the brain tissue sections of MCAO for 2
h and reperfusion for 22 h were stained by hematoxylin-eosin (H&E) for the morphological observation under microscope. Results Compared to the NS-treated group,the neurological deficit scores of CP-treated group after reperfusion for 8 h was decreased by (2.35±0.47)(P<0.05),in,company with the. Loss of infarct volume(P<0. 05 ). The rat cortical and hippocampal neuronal cell loss,gliosis,nuclear condensation and nuclear deep dyeing were significantly alleviated (P<0.05) .Conclusion The CP can attenuate of arterial embolism in rats and ameliorate cerebral ischemia, which possessing a neuroprolective effect.
【总页数】4页(P1109-1112)
【作者】唐焜;谢小慧;陈志达;陈扬;郭莲军
【作者单位】华中科技大学同济医学院附属同济医院第二临床学院,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030;华中科技大学同济医学院基础医学院药理学系,武汉430030
【正文语种】中文
【中图分类】R282.71;R285.5
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