去甲苔藓蒽噻吩抑制血管生成作用的体外实验研究(英文)

去甲苔藓蒽噻吩抑制血管生成作用的体外实验研究(英文) Chen Zhong;Xiangdong Zhou;Minghui Zhang;Yide Hu
【期刊名称】《中德临床肿瘤学杂志：英文版》
【年(卷),期】2011(10)2
【摘要】Objective:The aim of the study was to investigate the effect of Demethyl bryoanthrathiophene(DBT) on proliferations of human umbilical vein endothelial cells(HUVECs) and human lung adenocarcinoma cell line A549,and antiangiogenic effect of DBT on HUVECs in vitro.Methods:MTT assay was used to observe the effect of DBT on proliferations of HUVECs and A549 cells,flat plate scarification assay and tube formation in vitro test were used to observe the impact of DBT on migration and vaso-formed ability of HUVECs.The effects of DBT on apoptosis and cell cycle of HUVECs were calculated by flow cytometry.Results:MTT assay showed that treatment with DBT resulted in strong inhibition to the growth of HUVECs and A549 cells.The inhibition effects of DBT on HUVECs and A549 cells were related to dosage and times of dependency.In different doses of DBT(0.16,0.32 and 0.48 μmol/L) of flat plate scarification for 24 h,inhibition rates of DBT to migration of HUVECs were 14.70%,38.23% and
58.82%,respectively.In dose of DBT from 0.04,0.20 to 0.40 μmol/L for 24 h in tube formation,there were significance differences(P < 0.01) in the decreasing number of angiogenesis and incomplete blood vessel compared with control groups.All results showed that DBT promoted the
apoptosis rate of HUVECs,and the increase of concentration of DBT accompanied the acceleration of apoptosis rate.Conclusion:DBT could inhibit the proliferations of HUVECs and A549 cells,and effectively suppress angiogenesis in vitro.
【总页数】7页(P63-69)
【关键词】抗血管生成;体外增殖;DBT;血管内皮细胞;A549细胞;人脐静脉内皮细胞;细胞凋亡;MTT法
【作者】Chen Zhong;Xiangdong Zhou;Minghui Zhang;Yide Hu
【作者单位】Third Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;Department of Pharmacochemistry of Third Military Medical University, Chongqing 400037, China
【正文语种】中文
【中图分类】Q78;S884.21
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