一测多评法用于忍冬藤多成分含量测定的适用性研究
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2018 年 11 月第 25 卷第 11 期
中国中医药信息杂志
·77·
一测多评法用于忍冬藤多成分含量测定的适用性研究
田伟 1，2，3，甄亚钦 1，2，3，王鑫国 1，2，3，田宇柔 1，2，3，李军山 4，牛丽颖 1，2，3
1.河北中医学院，河北 石家庄 050091；2.河北省中药配方颗粒工程技术研究中心，河北 石家庄 050091； 3.河北省高校中药配方颗粒应用技术研发中心，河北 石家430
4. Shineway Pharmaceutical Co, Ltd, Shijiazhuang 051430, China Abstract: Objective To establish a QAMS method for content determination of six compositions (chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin) from Lonicerae Japonicae Caulis; To verify the feasibility and applicability of this method in quality control of Lonicerae Japonicae Caulis. Methods Chlorogenic acid was set as internal reference substance. The HPLC analysis was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase consisted of acetonitrile and 0.4% phosphoric acid solution in gradient elution manner at a flow rate of 1 mL/min. The column temperature was maintained at 35 ℃ , and the detection wavelength was set at 327 nm for chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and 236 nm for loganin. Results The relative correction factors of caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin were established; there was no obvious difference between calculated value of QAMS and measured value of external standard method. Conclusion The quality control mode of QAMS can be used for multi-index synchronization quality evaluation of the six compositions from Lonicerae Japonicae Caulis. Keywords: QAMS; relative correction factors; chlorogenic acid; Lonicerae Japonicae Caulis; quality evaluation
2. Hebei TCM Formula Granule Engineering＆Technology Research Center, Shijiazhuang 050091, China; 3. TCM Formula Granule Research Center of Hebei Provincial Universities, Shijiazhuang 050091, China;
关键词：一测多评；相对校正因子；绿原酸；忍冬藤；质量评价 DOI：10.3969/j.issn.1005-5304.2018.11.017 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2018)11-0077-06
Applicability Study on QAMS for Multi-component Content Determination of Lonicerae Japonicae Caulis
TIAN Wei1,2,3, ZHEN Ya-qin1,2,3, WANG Xin-guo1,2,3, TIAN Yu-rou1,2,3, LI Jun-shan4, NIU Li-ying1,2,3 1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China;
摘要：目的 建立一测多评法同时测定忍冬藤中绿原酸、咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C、 马钱苷 6 种成分含量的方法，并验证该法在忍冬藤质量控制中应用的可行性与适用性。方法 以绿原酸为内参 物，采用 Waters Symmetry C18 色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.4%磷酸，梯度洗脱，流速 为 1 mL/min，绿原酸、咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C 检测波长为 327 nm，马钱苷检测波长为 236 nm，柱温 35 ℃。结果 建立了忍冬藤中绿原酸与咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C、马钱苷 的相对校正因子；一测多评法的计算值与外标法实测值无明显差异。结论 一测多评的质量控制模式可用于忍 冬藤中 6 种不同成分的多指标同步质量评价。
中国中医药信息杂志
·77·
一测多评法用于忍冬藤多成分含量测定的适用性研究
田伟 1，2，3，甄亚钦 1，2，3，王鑫国 1，2，3，田宇柔 1，2，3，李军山 4，牛丽颖 1，2，3
1.河北中医学院，河北 石家庄 050091；2.河北省中药配方颗粒工程技术研究中心，河北 石家庄 050091； 3.河北省高校中药配方颗粒应用技术研发中心，河北 石家430
4. Shineway Pharmaceutical Co, Ltd, Shijiazhuang 051430, China Abstract: Objective To establish a QAMS method for content determination of six compositions (chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin) from Lonicerae Japonicae Caulis; To verify the feasibility and applicability of this method in quality control of Lonicerae Japonicae Caulis. Methods Chlorogenic acid was set as internal reference substance. The HPLC analysis was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase consisted of acetonitrile and 0.4% phosphoric acid solution in gradient elution manner at a flow rate of 1 mL/min. The column temperature was maintained at 35 ℃ , and the detection wavelength was set at 327 nm for chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and 236 nm for loganin. Results The relative correction factors of caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin were established; there was no obvious difference between calculated value of QAMS and measured value of external standard method. Conclusion The quality control mode of QAMS can be used for multi-index synchronization quality evaluation of the six compositions from Lonicerae Japonicae Caulis. Keywords: QAMS; relative correction factors; chlorogenic acid; Lonicerae Japonicae Caulis; quality evaluation
2. Hebei TCM Formula Granule Engineering＆Technology Research Center, Shijiazhuang 050091, China; 3. TCM Formula Granule Research Center of Hebei Provincial Universities, Shijiazhuang 050091, China;
关键词：一测多评；相对校正因子；绿原酸；忍冬藤；质量评价 DOI：10.3969/j.issn.1005-5304.2018.11.017 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2018)11-0077-06
Applicability Study on QAMS for Multi-component Content Determination of Lonicerae Japonicae Caulis
TIAN Wei1,2,3, ZHEN Ya-qin1,2,3, WANG Xin-guo1,2,3, TIAN Yu-rou1,2,3, LI Jun-shan4, NIU Li-ying1,2,3 1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China;
摘要：目的 建立一测多评法同时测定忍冬藤中绿原酸、咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C、 马钱苷 6 种成分含量的方法，并验证该法在忍冬藤质量控制中应用的可行性与适用性。方法 以绿原酸为内参 物，采用 Waters Symmetry C18 色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.4%磷酸，梯度洗脱，流速 为 1 mL/min，绿原酸、咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C 检测波长为 327 nm，马钱苷检测波长为 236 nm，柱温 35 ℃。结果 建立了忍冬藤中绿原酸与咖啡酸、隐绿原酸、异绿原酸 A、异绿原酸 C、马钱苷 的相对校正因子；一测多评法的计算值与外标法实测值无明显差异。结论 一测多评的质量控制模式可用于忍 冬藤中 6 种不同成分的多指标同步质量评价。