pGEX-His使用说明
重组蛋白的表达系统(详细版)
终止子：转录终止子按照是否依赖和不依赖ρ因子的作用分为两类，这两类终止子均在终止点前含有一段7-20bp的回文序列。终止子可以保护mRNA在核外不被降解，显著延长mRNA的寿命，由此提高重组蛋白的表达量。但是对于T7系统来说，由于T7 RNA聚合酶效率极高，宿主中随时都有充足的mRNA以供翻译，因此大部分在T7系统中表达的重组蛋白并不在意质粒上是否有终止子，只有一些自身带有翻译起始信号的外源基因需要终止子。启动子受细胞类型的限制，在不同的细胞系中有很大不同，因此需根据宿主细胞（尤其是真核宿主）的类型选择不同的启动子以便于目的基因的高效表达。
表4：常用原核表达载体质粒
1.3 优化表达条件
重组蛋白的表达流程很少有一次成形的，为了提高蛋白表达量、改善蛋白质量，表达条件和白不表达时：
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如果重组蛋白不表达（包含体和可溶蛋白都没有），首先检查cDNA和质粒是否正确，蛋白对宿主菌是否有很大毒性，然后尝试更换菌株、质粒载体和融合标签。原核蛋白在大肠杆菌中不能表达的情况很少见，通常是真核蛋白不能表达。不能表达的重组蛋白，即使在更换了宿主、载体后可以表达，表达量也不会很高，如果需要大规模生产，最好尝试酵母和昆虫细胞表达系统。
融合标签：融合标签是与目的蛋白共表达的一段多肽，方便重组蛋白的纯化、固定和检测，表3给出了常用的重组标签。如果不需要对重组蛋白进行纯化，尽量不要引入融合标签，以免影响蛋白性质；如果重组蛋白本身能够结合某种亲和柱，如某些金属结合蛋白可以结合Ni-NTA，某些糖结合蛋白能够特异识别糖类，也不必引入标签。融合标签的引入能够大大简化重组蛋白的纯化流程，并提高蛋白溶解度。商业化表达质粒，如pET、pGEX等提供了各种纯化标签和融合蛋白供选，应根据蛋白具体情况进行选择。His-tag是最常用的纯化标签，它具有很多优点：标签较短（10-20个氨基酸残基），不带电（pH8.0），免疫原性差，通常不影响重组蛋白的结构和功能，Ni2+亲和力高，能够通过一步纯化达到60%-90%的纯度。如果蛋白质溶解度不高，导致折叠困难、表达量低，可以选择较大的融合标签（GST、MBP、Trx等）帮助重组蛋白表达和折叠，提高重组蛋白溶解度，从而提高表达量。较大的融合标签有时也会导致翻译困难甚至提前中止，纯化后发现大部分都是标签蛋白也是常见现象。翻译的提前中止会大大影响重组蛋白产率和后续纯化，所以在短标签能够达到目的的时候，尽量不要选择大的融合标签。标签位置的选择也很重要：N端标签（短的或长的）自身带有启动子和适应宿主偏好的密码子，可以帮助目的蛋白表达，提高表达量，但是提前中止翻译的蛋白片段也会被一并纯化出来，降低重组蛋白纯度，对蛋白酶敏感的、自身容易降解的以及一级序列中有集中的疏水残基区的蛋白尤其要避免使用N端标签；C端标签则可以保证只有完整蛋白得到纯化。另外，如果蛋白的近N端或近C端有重要功能区，如酶活中心、配体结合位点、二硫键、多聚体稳定界面、相互作用界面等，则要避免纯化标签位于该末端，以免影响重组蛋白的结构和功能。如果融合标签对蛋白性质有较大影响，但又是纯化所必须的，就可以考虑在纯化过程中去除标签。主要有三种方法：化学裂解，如溴化氰（CNBr）、羟胺（NH2OH）等，能够简单有效地去除标签，但反应条件苛刻（羟胺需要在pH9.0下反应），特异性较差，而且会引入不必要的修饰，除包含体蛋白的处理外已经很少使用了；酶解，如PPase等，其底物一般是一段比较长的肽链，特异性强，是目前比较常用的方法，缺点是酶切反应需要较长的时间，也增加了蛋白纯化的步骤，使纯化变得繁琐；IMPACT质粒，该质粒在纯化标签和目的蛋白之间插入了一个蛋白质内含子（intein），intein具有可诱导的自切割活性，使用IMPACT质粒表达的重组蛋白，只需要改变缓冲液的pH和温度，即可切掉融合标签。
第六章抗体的表达
常用的原核表达载体分类及特征
转录载体：用以表达本身带有原核核糖体结合位 点和AUG起始密码子的目的基因T-24（+）和pET-23（+）
体
翻译载体：包括来自T7噬菌体主要衣壳蛋白的高效
核糖体结合位点，用于表达一些不带有核糖体结合
位点的目的基因
pET载体系统为满足不同的需求，生产了带有His·Tag、 T7·Tag、S·Tag、GST·Tag、ompT、CBD S·Tag、 Thioredoxin、DsbA·Tag 、Trx·Tag等标签的融合蛋白，其中带 有S·Tag、His·Tag、T7·Tag的蛋白易于通过蛋白质杂交检测。
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一种表达体系的核心是表达载体及宿主菌，而表达方法的进步也 主要体现在载体元件的优化和宿主菌基因型的改造上。 理想的原核表达载体应具有以下特征：
（1）稳定的遗传复制和传代能力；
（2）具有显性的转化筛选标记； （3）启动子的转录是可调控的，抑制时本底转录水平较低； （4）启动子转录的mRNA能够在适当的位置终止，转录过程 中不影响表达载体的复制； （5）具备适用于外源基因插入的酶切位点，以确保目的基因 按一定方向与载体正确衔接表达产物的分离、纯化。
第六章抗体的表达
第一节 概述
目前抗体的主要类型： 1、多克隆抗体：通过免疫手段制备。 2、单克隆抗体：通过杂交瘤技术制备。 3、基因工程抗体：包括嵌合抗体、改型抗体等，通过 基因工程手段制备，涉及设计、构建与表达等。
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要想获得全长的抗体分子，就必须选择恰当的抗体表 达系统，而要使所表达的蛋白质结构不具有免疫原性 或避免半衰期过短，就需要使用一个标准的抗体工程 技术生产全人抗体。
大肠杆菌表达系统昆虫细胞表达系统哺乳动物表达系统酵母表达系统存在一些蛋白不能有效地折叠发酵周期较长容易污染需要考虑表达产物的卫生安全性筛选产物价格昂贵有时表达产物没有生物活性等问题都为临床生产和应用带来了不便也存在产物低表达或不表达的问题产物纯化问题依然存外源基因不能持久稳定地表达而且表达成本很高技术背景复杂存在不正确的糖基化修饰且表达量低原核表达系统具有吸引力的原因在于它的成本低生产率高和能够大规模快速的生产等优点第二节原核表达系统原核表达是指通过基因克隆技术将外源目的基因通过构建表达载体并导入表达菌株的方法使其在特定原核生物或细胞内表达
PGX-操作手册-C
PGX型接触角型表面张力仪操作手册版本：3.4目录1.打开仪器包装2.技术要求3.安装PGX操作软件4. 校正和确认4.1 校正板和2个钢球：4.2 校正板带1个钢球：4.3 确认步骤5.装配PGX仪器5.1准备标本5.2 特殊标本5.3泵分配器6.杂项6.1样品制备6.2建立一个测试6.3设备储存6.4运输7.测试模式7.1静态模式的应用7.2静态模式的自动测试7.3动态模式滴落应用7.4动态模式中的自动测试7.5检测前进/后退接触角8.报道9. 袖珍接触角方案9.1图像控制面板9.2主程序9.3静态模式9.4 动态模式9.5 在静态/动态模式手动测试9.6 前进模式9.7 超低接触角9.8 查看模式9.9 表面张力9.10 表面“自由”能（SFE）9.11 选择屏幕9.12 加强模式9.13 基线测定9.14 相机增益和阈值9.15 视野限制9.16 使用其它测试液体来确定“表面自由能”9.17 用户界面的翻译9.18 系统参数（选择菜单）10．消耗品11 故障排除附录：A.1 推荐液滴尺寸大小：A.2 检测动态模式下的液滴：B.1 经验性问题：C.1 表面能量换算表：D.1 更换泵管D.2 调整泵容量：D.3 低于0.5 μl的小液滴：重要提示(关于相机芯片ST672 and ST676)：PGX型仪器所用的原始相机芯片ST672通过透明导线的USB接口转换来实现。
后来该芯片被ST676所替代，被换成黑色USB导线。
ST676能提供ST672所没有的一些功能。
PG 系列的软件能支持这两个芯片，它能自动在您的仪器里识别。
两者的不同之处可通过相关文件来描述；1.打开仪器包装：仪器箱子里面有以下组成部分：仪器主机1个；英文操作手册1本；CD软件1个；微量注射器1个；校正板1个；玻璃瓶1个；2.技术要求：PGX操作软件可在以下Windows环境下使用：Windows98，Windows2000，Windows XP，Windows ME；图1.2 PGX配件包请注意：PGX仪器不能在Windows NT下安装，因为Windows NT操作系统不支持USB接口应用；3.安装PGX操作软件：该CD软件被放在仪器主机的下面；请注意：即使Windows建议，也不允许从网络中下载任何相机的驱动程序，因为这样的话仪器运行不具有可靠性；请注意：只有在软件安装完毕后，才允许将PGX仪器主机上USB接口插在电脑上；否则，Windows将选择别的驱动器，其结果不可预测；a)往电脑中插入CD来安装操作软件，可根据界面的提示操作。
X4使用说明
Impulse X4 系列便携式复合气体检测仪操作说明书！重要提示：！在首次使用仪器以前请认真阅读本手册，您将会掌握仪器正确的使用方法和了解仪器的功能，包括操作，维护，功能设置等内容。
！为了使操作者更安全，请按照手册中的要求，定期对仪器进行标定。
！如果在使用过程中，遇到的故障或问题在本手册中没有提到，请直接联系制造商Zellweger Analytics，或联系当地的代理商/服务商。
！警告和注意：·更换任何元器件都有可能损坏仪器的本质安全结构。
·如果需要使用存储卡，请选用Zellweger Analytics 提供的存储卡（订货号2566-0435），使用其它的存储卡有可能损坏仪器的本质安全结构。
·在允许的储存期之后激活检测器，有可能影响仪器的使用性能和保质期。
·应使用许可的5号干电池，如劲量电池，不要使用质量低下的干电池，以免影响仪器的本质安全性能。
·在更换电池时，应同时更换2节型号相同的新电池。
·在电池欠压提示后，应尽快更换新电池，以免旧电池漏液损坏仪器。
·在低温环境下，电池的寿命会缩短。
·更换电池时，应该在安全环境下进行。
·当更换任何一个传感器的情况下，都需要对仪器进行标定。
·在每天使用以前，应完成仪器的自检过程。
·定期的对仪器用标气进行测试，检查声、光、振动报警是否正常。
·标定时应选用厂家或国家认证合格企业提供的标准气体。
·标定时应在良好通风的环境下进行，以避免污染。
·不要在仪器电量不足的情况下标定。
·不要在富氧的环境下使用本仪器。
·可燃气体传感器的灵敏度会受到高浓度硫化物，卤素化合物，含硅化合物，以及含铅气体或蒸汽的影响，也叫“中毒”，应避免在以上的环境中使用仪器，如果必须使用，则使用完后应对仪器进行检测和标定，以免影响以后的使用。
上海实验室：上海市徐汇区银都路218号 聚科生物
北京实验室：北京亦庄经济技术开发区荣昌东街7号隆盛工业园203邮编：100088 电话：800-820-8982-1-1 Email：seqbj@
广州实验室：广州市海珠区新港东路2429号海珠科技大楼 213室邮编：510320 电话：800-820-8982-1-1 Email：seqgz@
常用载体与引物查询表
北京实验室：北京亦庄经济技术开发区荣昌东街7号隆盛工业园203邮编：100088 电话：800-820-8982-1-1 Email：seqbj@
广州实验室：广州市海珠区新港东路2429号海珠科技大楼 213室邮编：510320 电话：800-820-8982-1-1 Email：seqgz@
北京实验室：北京亦庄经济技术开发区荣昌东街7号隆盛工业园203邮编：100088 电话：800-820-8982-1-1 Email：seqbj@
广州实验室：广州市海珠区新港东路2429号海珠科技大楼 213室邮编：510320 电话：800-820-8982-1-1 Email：seqgz@。
重组蛋白质的分离纯化 (1)
重组蛋白质的分离纯化摘要：90年代以来基因重组技术得到很大的发展，基因工程产品的分离纯化的成本约占其全部成本的60%～80%，因此重组蛋白的分离纯化技术越来越重要。
本文主要介绍了沉淀、液液萃取、层析等常用分离重组蛋白方法的原理及应用，旨在为开展蛋白质的制备及其应用研究提供理论依据。
关键词：重组蛋白质；分离；纯化；沉淀；液液萃取；层析；包涵体随着基因重组技术的发展，出现了很多基因工程产品，而作为基因工程技术的下游工程中的基因重组蛋白的分离纯化技术越来越显示其重要性。
据有人统计,基因工程产品的分离纯化成本约占到其全部成本的60%～80%[1]。
由此可见产品的分离纯化是获得目的产物的关键一步，也是比较困难的一步，它标志着生物产业的高低。
纯化重组蛋白质和普通蛋白质的不同就在于要选择合适的表达系统，因为表达系统决定了细胞培养过程中产物的性质以及可能产生的杂蛋白，而纯化重组蛋白质的主要目的是去除杂蛋白质，通常对一种重组蛋白质的纯化会采用多个系统[2]。
但是重组蛋白有几种不同的表达形式，如细胞外的分泌表达；细胞内可溶性表达以及包涵体形式的存在，因此对于重组蛋白的纯化要依据其表达形式的不同，采取不同的纯化工艺。
与传统方式相似，重组蛋白的分离纯化也是利用其物理和化学性质的差异，即以分子的大小、形状、溶解度、等电点、亲疏水性以及与其它分子的亲和性等性质建立起来的。
目前主要的纯化方法有浓缩沉淀法，层析和电泳技术。
重组蛋白质在分离纯化的过程中，必须维持一定的浓度和生物活性形式，以及防止被降解。
因此从生物体中有效分离纯化重组蛋白质一直是个难题。
90 年代以来,国内外许多科学工作者在蛋白质分离纯化技术和工艺上进行了大量的研制和开发,将原有的纯化技术水平提高到一个新的高度。
本文将简单介绍一些传统的分离纯化方法，并介绍近10 年来重组蛋白分离纯化中的新进展和一些新出现的技术。
1 沉淀分离技术1.1 盐析法其原理是蛋白质在高浓度盐溶液中，随着盐浓度的逐渐增加，由于蛋白质水化膜被破坏、溶解度下降而从溶液中沉淀出来。
EMSA操作方法(原核表达纯化蛋白)
三、凝胶滞留试验（EMSA）凝胶迁移滞留试验（electrophoretic mobility shift assays，EMSA）是研究核酸与蛋白质相互作用简单、快速、敏感的方法，目前已经成为转录因子研究的经典方法，并且用于研究RNA结合蛋白和特定的RNA序列的相互作用。
其基本原理是蛋白质可以与末端标记的核酸探针结合，电泳时这种复合物比无蛋白结合的自由探针在凝胶中泳动的速度慢，即表现为相对滞后。
（一）转录因子-标签融合蛋白和标签蛋白的原核表达纯化以GST标签为例。
裂解液：25mM pH7.8Tris-HCl、100mM NaCl、2 mM NaEDTA、1mM DTT和21×Complete Protease InhibitorCocktail。
洗脱液：50 mMpH7.8Tris-HCl、200mM NaCl、1mM DTT、0.02%（v/v）Triton X-100和10 mM还原性谷胱甘肽。
EDTA、1mM DTT和1×Complete 透析液：25mM pH8.0 Tris-HCl、1mM Na2Protease InhibitorCocktail。
1将转化了pGEX-4T-2载体或者pGEX-4T-2-目的基因重组载体的BL21大肠杆菌在5 mL含100 µg mL-1氨苄青霉素的LB培养液中37℃、200 rpm震荡培养12 h。
2 取1 mL菌液加入100 mL 含100 µg mL-1氨苄青霉素的LB培养液中，37℃、200 rpm震荡培养至OD600为0.5左右。
3 加入IPTG至终浓度为0.5 mM，28℃、200 rpm培养6 h。
4 冰上放置10 min，4℃、3000×g离心15 min，收集菌体并称重。
5 每克菌体加入3 mL裂解液，重悬菌体，加入溶菌酶至终浓度为0.5 mgmL-1，在冰上温和地搅动菌液30 min。
质粒图谱大全
转载一.九种表达载体Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbA 二.克隆载体pTZ19RDNApUC57DNAPMD18TPQE30pUC18pUC19pTrcHisApTrxFuspRSET-ApRSET-BpVAX1PBR322pbv220pBluescriptIIKSL4440pCAMBIA-1301pGD926三.PET系列表达载体ProteinExpressionProkaryoticExpressionpETDsbFusionSystems39band40b ProteinExpressionProkaryoticExpressionpETExpressionSystem33b ProteinExpressionProkaryoticExpressionpETExpressionSystems ProteinExpressionProkaryoticExpressionpETExpressionSystemsplusCompetentCells ProteinExpressionProkaryoticExpressionpETGSTFusionSystems41and42 ProteinExpressionProkaryoticExpression ProteinExpressionProkaryoticExpressionpETVectorDNA ProteinPurificationPurificationSystemsStrepTactinResinsandPurificationKits四.PGEX系列表达载体TEcoR pGEX-1I/BAPpGEX-2TpGEX-2TKpGEX-3XpGEX-4T-1pGEX-4T-2pGEX-4T-3pGEX-5X-1pGEX-5X-3 pGEX-6P-1 pGEX-6P-2 pGEX-6P-3五.PTYBsystem PTYB1PTYB2PTYB11PTYB12六.真核表达载体-pPICZalphaA pGAPZαApBI121pEGFP-N1 pEGFP-C1pPIC9K如何阅读分析质粒图谱载体主要有病毒和非病毒两大类,其中质粒DNA是一种新的非病毒转基因载体;一、一个合格质粒的组成要素复制起始位点Ori 即控制复制起始的位点;原核生物DNA分子中只有一个复制起始点;而真核生物DNA分子有多个复制起始位点;抗生素抗性基因可以便于加以检测,如Amp ,Kan多克隆位点MCS克隆携带外源基因片段P/E 启动子/增强子Terms终止信号加polyA信号可以起到稳定mRNA作用二、如何阅读质粒图谱第一步：首先看Ori的位置,了解质粒的类型原核/真核/穿梭质粒第二步：再看筛选标记,如抗性,决定使用什么筛选标记;1Ampr水解β－内酰胺环,解除氨苄的毒性;2tetr可以阻止四环素进入细胞;3camr生成氯霉素羟乙酰基衍生物,使之失去毒性;4neorkanr氨基糖苷磷酸转移酶使G418长那霉素衍生物失活5hygr使潮霉素β失活;第三步：看多克隆位点MCS;它具有多个限制酶的单一切点;便于外源基因的插入;如果在这些位点外有外源基因的插入,会导致某种标志基因的失活,而便于筛选;决定能不能放目的基因以及如何放置目的基因;第四步：再看外源DNA插入片段大小;质粒一般只能容纳小于10Kb的外源DNA片段;一般来说,外源DNA片段越长,越难插入,越不稳定,转化效率越低;第五步：是否含有表达系统元件,即启动子－核糖体结合位点－克隆位点－转录终止信号;这是用来区别克隆载体与表达载体;克隆载体中加入一些与表达调控有关的元件即成为表达载体;选用那种载体,还是要以实验目的为准绳;启动子－核糖体结合位点－克隆位点－转录终止信号启动子－促进DNA转录的DNA顺序,这个DNA区域常在基因或操纵子编码顺序的上游,是DNA 分子上可以与RNApol特异性结合并使之开始转录的部位,但启动子本身不被转录;增强子/沉默子－为真核基因组包括真核病毒基因组中的一种具有增强邻近基因转录过程的调控顺序;其作用与增强子所在的位置或方向无关;即在所调控基因上游或下游均可发挥作用;/沉默子－负增强子,负调控序列;核糖体结合位点/起始密码/SD序列Rbs/AGU/SDs：mRNA有核糖体的两个结合位点,对于原核而言是AUG起始密码和SD序列;转录终止顺序终止子/翻译终止密码子：结构基因的最后一个外显子中有一个AATAAA的保守序列,此位点down－stream有一段GT或T富丰区,这2部分共同构成polyA加尾信号;结构基因的最后一个外显子中有一个AATAAA的保守序列,此位点down－stream有一段GT或T 富丰区,这2部分共同构成polyA加尾信号;回答有人之前提出的一个问题：为什么质粒图谱上有的箭头顺时针有的箭头逆时针,那其实是代表两条DNA链,即质粒是环状双链DNA,它的启动子等在其中一条链上,而它的抗性基因在另一条链上.三、介绍一下关于载体的知识虽然课本上都有写1. 什么是载体即要把一个有用的基因目的基因——研究或应用基因通过基因工程手段送到生物细胞受体细胞,需要运载工具交通工具携带外源基因进入受体细胞,这种运载工具就叫做载体vector;.基因工程所用的vector实际上是DNA分子,是用来携带目的基因片段进入受体细胞的DNA2. 载体的分类―――按功能分成：1克隆载体都有一个松弛的复制子,能带动外源基因,在宿主细胞中复制扩增;它是用来克隆和扩增DNA片段基因的载体;所以有时实验时扩增效率低下,要注意是不是使用的严谨行载体2表达载体具有克隆载体的基本元件ori,Ampr,Mcs等还具有转录/翻译所必需的DNA顺序的载体;―――按进入受体细胞类型分：1原核载体2真核载体3穿梭载体sbuttlevector指在两种宿主生物体内复制的载体分子,因而可以运载目的基因穿梭往返两种生物之间..穿梭质粒含原核和真核生物2个复制子,以确保两类细胞中都能扩增;3. 基因工程载体的3个特点：一都能独立自主的复制：载体DNA分子中有一段不影响它们扩增的非必需区域,如MCS,插在其中的外源DNA片段,能被动的跟着载体一起复制/扩增,就像载体的正常成分一样;二都能便利的加以检测：如载体的药物抗性基因,多是抗生素抗性基因,将受体细胞放在含有该抗生素培养板上培养生长时,只有携带这些抗性基因的载体分子的受体细胞才能存活;三都能容易进入宿主细胞中去,也易从宿主细胞中分离纯化出来;4. 载体的选择和制备：选择载体主要依据构建的目的,同时要考虑载体中应有合适的限制酶切位点;如果构建的目的是要表达一个特定的基因,则要选择合适的表达载体;载体选择主要考虑下述3点：1构建DNA重组体的目的,克隆扩增/表达表达,选择合适的克隆载体/表达载体;2.载体的类型：1克隆载体的克隆能力－据克隆片段大小大选大,小选小;如<10kb选质粒;2表达载体据受体细胞类型－原核/真核/穿梭,哺乳类细胞表达载体;3对原核表达载体应该注意3点：①选择合适的启动子及相应的受体菌；②用于表达真核蛋白质时注意克服4个困难和阅读框错位；③表达天然蛋白质或融合蛋白作为相应载体的参考;3载体MCS中的酶切位点数与组成方向因载体不同而异,适应目的基因与载体易于链接,不产生阅读框架错位;选用质粒最常用做载体的4点要求：①选分子量小的质粒,即小载体1－→不易损坏,在细菌里面拷贝数也多也有大载体；②一般使用松弛型质粒在细菌里扩增不受约束,一般10个以上的拷贝,而严谨型质粒<10个;③必需具备一个以上的酶切位点,有选择的余地；④必需有易检测的标记,多是抗生素的抗性基因,不特指多位Ampr试一试;无论选用哪种载体,首先都要获得载体分子,然后采用适当的限制酶将载体DNA进行切割,获得分子,以便于与目的基因片段进行连接。
HIS使用说明书
第1章HIS系统操作说明 (4)1登陆操作 (4)1.1登陆界面 (4)2门诊挂号 (5)2.1系统维护 (5)2.2挂号操作 (8)2.3挂号查询 (10)2.4挂号报表 (10)2.5病人个人信息 (12)3门诊收费 (13)3.1系统维护 (13)3.2门诊收费 (14)4门诊医生工作站 (17)4.1系统维护 (17)4.2门诊医生 (18)5办理入出院操作 (28)5.1办理入院 (28)5.2出院结账 (29)6住院医生工作站 (31)7门诊(中西)药房管理子系统 (37)8药库房管理子系统 (39)1药品入库 (39)2药品入库修改 (40)3药品出库 (41)4库房药品调节 (41)5药品调价 (42)6药品购买计划 (43)7出库查询 (44)8库房药品调节查询 (45)9库房盘存表 (45)10出入库统计报表 (45)11库房系统维护 (45)第2章LIS操作说明 (46)1小结 (47)2日常操作 (47)2.1系统登录 (47)2.2检验 (47)2.2.1病人信息录入 (47)2.2.2项目信息录入 (49)2.2.3申请单批量录入 (49)2.2.4检验结果传输 (50)2.2.5检验报告生成 (50)2.2.6申请批量录入 (50)2.2.7检验报告审定 (51)2.2.8检验报告打印 (51)2.3查询 (51)2.3.1检验报告查询 (51)2.4质控 (52)2.4.1查看质控数据 (52)2.4.2查看质控图： (52)2.4.3质控图打印 (52)3软件安装与设置 (52)3.1软件安装与删除 (52)3.1.1出厂安装 (52)3.1.2软件重新安装 (53)3.1.3软件使用授权 (54)3.1.4数据设置 (55)3.1.5项目设置 (56)3.1.6仪器设置 (61)3.1.7小组设置 (63)3.1.8用户设置 (63)3.1.9质控设置 (64)4常见问题及处理方法 (66)4.1通讯问题 (66)4.11当天数据突然不传送 (66)4.12接到的数据为乱码 (67)4.13开启中文电脑时,影响仪器检测结果 (67)4.14某一个样本或项目无法传输过来 (67)4.15主程序正常启动,通信程序(红色窗口)未启动. (67)4.16系统不对原始数据进行解释 (67)4.17质控结果传过来,但在质控界面中看不到 (68)4.18跳出提示”WIN232,管道正在关闭中.....” (68)4.19登录时,主界面功能都是灰色的,不能正常操作使用 (68)4.1.10通讯程序无法授权 (68)4.1.11检验界面看不到录入的单子 (68)4.1.12登陆时候有错误提示 (68)4..1.13检验之星的检验界面看不到任何传过来的数据 (69)4.1.14通讯程序对传过来的数据不进行解释 (69)4.2打印问题 (69)4.2.1打印时,点打印按纽不打印报告单或走纸不正常 (69)4.2.2有些检验项目设置完参考值范围后,不打印参考值范围 (69)4.2.3只打出报告的框架,而不能打出项目的结果 (69)4.2.4只打印几行怪字符,并走很长的一段空纸 (69)4.2.5打印几行怪字符后才打印报告单 (69)4.2.6报告的标题或页尾不打印出来 (70)4.2.7打印的第一张报告单主标题如:”*******检验报告单”不清楚 (70)4.2.8打印出来的全部为乱码 (70)4.2.9 报错打印失败 (70)4.3接口问题 (70)4.3.1某一个申请单核收不到 (70)4.3.2某一个项目核收不到 (70)4.3.3核收过来是乱码 (70)4.3.4核收界面显示无法显示该网页 (70)4.3.5在核收时，核收不到数据 (70)4.3.6重审修改数据报错，写入HIS数据库失败 (71)4.3.7在接口配置中，连接ORACLE客户端时显示连接不上，但是直接用SQLPLUS可以连上客户端 (71)4.3.8在审定时出现如下图的错误 (71)4.3.9在接口配置或核收界面中，有住院、门诊或体检中的一个或几个界面显示不出来 (71)4.3.10当在基础对照，或别的对照中显示不出页面 (71)4.3.11在住院核收数据时，医生可以核收到，并可以在检验界面中显示；但是在门诊中核收不到医生，“HIS医生无法转换” (71)4.3.12在核收界面都显示正确，保存没报错，但是在检验界面找不到核收的数据 (72)4.4其他问题 (72)4.4.1某一个计算项目没有计算 (72)4.4.2启动软件时报错“程序未经授权” (72)4.4.3维护里数据更改过,但不能马上使用 (72)4.4.4有一些报告审核不了 (72)4.4.5在”检验”项目中,发现有些检验项目没有结果,但有结果异常提示 (73)4.4.6提示”数据库初始化错误” (73)4.4.7所有用户都无法登陆,报”密码错误” (73)4.4.8出现”主数据库连接错误” (73)4.4.9启动软件时报日期错误 (73)4.4.10打开软件时跳出提示“ptech.exe产生错误......” .. (73)第3章体检系统 (74)1业务流程 (74)1.1确定体检项目 (74)1.2收费 (74)1.3逐个科室检查 (74)1.4总检 (74)1.5输出报告 (74)2功能介绍 (74)2.1基础数据维护 (74)2.2检前准备 (75)2.3体检管理 (76)2.4查询 (76)第1章HIS系统操作说明1登陆操作1.1登陆界面图1-1每个操作用户都会有一个操作用户名，在操作使用模块式时打开应用程序就会弹出如图1-1 这样的窗口。
蛋白体外表达与纯化
蛋白体外表达与纯化随着后基因组时代的到来,蛋白质组成为科学研究的热点。
蛋白质作为生命机体的主要活动的承担者,其体外表达与纯化在研究相应基因的功能上有重要意义。
蛋白体外表达系统按其表达宿主可分为原核表达系统,真核表达系统和哺乳动物细胞表达系统。
一:原核表达系统原核表达系统的宿主菌主要以大肠杆菌为代表,大肠杆菌表达体系是目前应用最广泛的外源基因表达体系,这也是外源基因表达的首选体系。
该表达体系的优点:遗传学和生理学背景清楚;容易培养;外源基因经常可以高效表达及操作简单、周期短、成本低等。
其不足之处是不能进行典型真核细胞所具有的复杂的翻译后修饰;广泛的二硫键的形成及外源蛋白组装成蛋白复合体的能力也受到限制;另外外源基因产物在大肠杆菌中易形成不溶的包涵体;有时由于真核mRNA的结构特性及密码子使用频率与大肠杆菌的差异,而的不到足够的产物。
二:真核表达系统真核表达系统的宿主菌主要以酵母表达系统为代表,酵母基因表达系统的载体通常既能在酵母中进行复制也能在大肠杆菌中进行复制,形成所谓酵母菌――大肠杆菌穿梭载体。
因以大肠制备质粒DNA较方便,通常利用大肠杆菌系统构建酵母载体以简化手续,缩短时间。
作为基因表达系统的宿主应该具备以下条件:安全无毒,不致病;遗传背景较清楚,容易进行遗传操作;容易进行载体DNA的导入;培养条件简单;有良好的蛋白分泌能力;有类似高等真核生物的蛋白翻译后修饰功能。
三:哺乳动物细胞表达系统由于本专业不涉及哺乳动物细胞表达系统的应用,故此不赘述。
表达载体的种类及相应的分离纯化方法作为表达载体必须具备以下特征:稳定的遗传复制、传代能力,无选择压力下能存在于宿主细胞内;具有显性的筛选标记;启动子的转录是可调控的;启动子的转录的mRNA能够在适当的位置终止;具有外源基因插入的多克隆位点。
在原核表达系统中常用的表达载体有:PET-载体系列,用这类载体表达出的外源蛋白在N 端或C端或两端均具有his tag。
大肠杆菌基因表达系统
必须用cDNA或者是化学合成的基因。 外源基因不能带有内含子。
除了具备克隆载体具备的条件外，还应具备以下条件： 强启动子、强转录终止子 可诱导性 合适的核糖体结合位点和起始密码ATG等
理想原核表达载体具备的基本特征
1
2
5
4
3
A dividing E. coli
原核或噬菌体启动子
MCS
SD序列
终止子
条件：
组成结构：
必须选择一个有lacI的宿主菌。
tac P
Lac O
S导物： IPTG（乳糖的类似物，不会被降解）
阻遏物
IPTG
2.分泌型表达载体
如：pIN III系列： 载体表达出的外源蛋白质与细菌的分泌信号肽连在一起，可被宿主菌分泌到细胞周质中。 pIN III-A1, pIN III-A2, pIN III-A3,
EcoR I
BamH I
凝血酶
Ile Giu Gly Arg Gly Ile Pro Gly Asn Ser Ser
EcoR I
BamH I
Xa因子
pGEX-3X的插入区：
Sma I
Sma I
其他融合蛋白系统
His-tag能与Ni2+柱结合，但很容易被EDTA（或咪唑溶液）洗脱下来，可以纯化蛋白质。
细胞质
外膜
内膜
周间质
质粒
染色体
“外排”： 蛋白质从细胞质跨过内外膜到培养液中。 “分泌”： 蛋白质从细胞质跨过内膜到周间质中。
01
碱性氨基酸
Leu、Ile
Arg、Lys
02
N
04
疏水氨基酸核心区
07
AlaGlySer
Extech 品牌的湿度 温度数据日志器说明书
OrderingRH520A ...........Humidity+T emperature Chart Recorder (110V adaptor)RH520A-NIST .RH520A with NIST CertificateRH520A-220....Humidity+T emperature Chart Recorder (220V adaptor)RH520A-240....Humidity+T emperature Chart Recorder (240V adaptor)RH522..............Replaceable Humidity/Temperature Probe SL123...............AC Alarm Relay Module 9ft (3m)cable SL124...............DC Alarm Relay Module 9ft (3m)cableRH520A Humidity+Temperature Chart RecorderBest value graphical datalogger in the market that’s easy to set up and operateFeatures:•Simultaneous numerical &graphical display of Humidity and T emperature readings,plus Time and Date •Large dual graphical LCD displays with adjustable vertical and horizontal resolution indicate %of memory remaining•Internal memory records up to 49,000data points can be transferred to a PC •Detachable probe extends to 3ft (1m)•Audible and visual alarm with Hi/Low setpoints •Output socket used withoptional external alarm module •Complete with built-in stand,detachable probe with 3ft (1m)cable,RS232cable,RS-232to USB adaptor,LabView-based PC software,100-240VAC 50/60Hz adaptor,and 3AA batteriesWall mountablePC software control and Humidity,ambient point,wet bulb,or g/kg)Monitors Humidity and Temperature levels in warehouse,storage rooms,freezers,shipping vans,and B connector easily plugs into a computer for data analysis.Features:•USB interface for easy setup and data download to a PC•Dew point indication via Windows ®compatible software (included)•Selectable data sampling rate:2s,5s,10s,30s,1m,5m,10m,30m,1hr,2hr,3hr,6hr,12hr,24hr•User-programmable alarm thresholds for RH and T emperature•LCD displays current readings,Min/Max,and alarm status •Long battery life (approx.1year)•Complete with 3.6V Lithium battery,mounting bracket with combination lock,and Windows ®98,2000,XP ,and Vista compatible analysis softwareOrdering(Available Q42009)RHT20..........Humidity and T emperature DataloggerRHT20Humidity and Temperature DataloggerDatalogs 16,000Humidity and 16,000Temperature readingsFeatures:•42265—Waterproof (IP65)T emperature Datalogger Kit records up to 8000readings •42275—Water resistant Temperature and Humidity Datalogger Kit records up to 16,000readings (8,000readings/parameter)•Real time clock with date and time •Logs data for days,weeks or months •Three start methods:Scheduled,immediate or magnetic•Multiple Dataloggers (42260or 42270)can be programmed and data downloaded from one single docking station•Programmable sampling rate from 1sec to 2hrs plus Hi/Lo limits with alarm indicationFeatures:•Programmable from keypad or PC •Selectable data sampling rate•User programmable audible and visual alarms •Wall,desktop,or tripod mount •Calibration via optional salt bottlesOrdering42280............Temperature and Humidity Datalogger RH300-CAL ..33%and 75%RH Calibration Kit42265/42275Temperature &Humidity Datalogger KitsChoice of Temperature/Humidity or Temperature only Datalogger —Compact,powerful and easy to use with PC interfaceSoftware includedComplete with Windows ®compatible software,USB cable,110V AC adaptor,and four AA batteriesRHT10/TH10Humidity &Temperature USB DataloggersTwo models to choose from with USB interface for easy set-up and data downloadFeatures:•Model RHT10datalogs 16,000Temperature/16,000RH readings;Dew point indication via Windows ®compatible softwareRHT10-SW software for RHT10to calculate Grains Per Pound (grams per kilogram)to 1120GPP (160g/kg)Model TH10datalogs 32,000T emperature readings•Selectable data sampling rate:2s,5s,10s,30s,1m,5m,10m,30m,1hr,2hr,3hr,6hr,12hr,24hr•User-programmable alarm thresholds for RH and Temperature •Humidity:0to 100%RH (Model RHT10only)Temperature:-40to 158°F (-40to 70°C);Accuracy:±3%RH;±1°F/0.5°C •1yr battery life;Dims:5.1x1.1x0.9"(130x30x25mm)/Wt:1oz(20g)Ordering42275..........T emperature/Humidity Datalogger Kit with PC Interface 42270..........T emperature/Humidity Datalogger for 4227542265..........T emperature Datalogger Kit with PC Interface 42260..........T emperature Datalogger for 4226542299..........Replacement 3.6V Lithium batteries (2pk)USB100.......RS-232to USB AdaptorDatalogger Kit available in two configurations:42265includes one 42260datalogger.42275includes one 42270datalogger.Both includeWindows ®compatible software &docking station.42280Temperature andHumidity DataloggerRecords up to 16,000readings with USB interface for downloading to a PCOrderingRHT10........Humidity and T emperature USB Datalogger RHT10-SW .GPP (g/kg)Software for RHT10TH10..........Temperature USB DataloggerSoftware includedComplete with 3.6V Lithium battery,protective USB cap,mounting bracket,and Windows ®compatible software for analysisOrder with Digital PsychrometersNew humidity and temperature meters with built-in or remote probes include wet bulb and dew point measurements for quick assessment of air moisture content.Only Extech offers Dew Point Meters withDifferential and IR Thermometermeasurementscontent without slingingRH355Value KitKit includes AccessoriesRH300-CAL ....33%and 75%RH Calibration Kit for RH300RH350-CAL ....33%and 75%RH Calibration Kit for RH350RH401-CAL TP890...............Thermistor 407752.............PC 589250.............RS-232USB100............RS-232RH355Kit —includes RH350,33%and 75%RH305Kit —includes RH300,temperature probe,RH405Kit —includes RH401with 33%and 75%calibration USB port,cable and PC software includedAccessoriesHumidity&Temperature MetersExtech’s Humidity/Temperature meters are available in the style to fitthe user’s measurement needs:remote probe for easy access intoducts and vents,and integral probefor convenient one hand operation.Kit includes calibrationstandards and case Ideal for use in sporting eventsRH101 Thermometer Humidity,Airfor non-contact measurements Non-contact Temperature measurements。
Shure PGXD 无线系统用户指南说明书
PGXDWireless SystemShure online user guide for PGX Digital wireless systems. Version: 2.1 (2022-E)Table of ContentsPGXD Wireless System3 Quick Start3 General Description6 6 System Components 7 All systems include 7 Vocalist systems include 7 Lavalier, Headworn, and Instrument systems include 7 Guitar systems include 7 Receiver Controls and Connectors7 Transmitter Controls and Connectors 8 Transmitter Indicator LED9 Batteries10 Wearing the Bodypack Transmitter10 Adjusting Levels 11Adjusting Gain11 Bodypack Transmitter 11 Handheld Transmitter 12 Single System Setup 12 Multiple System Setup13 Compatible Frequencies 13 Manual Channel and Group Selection (receiver only) 14 Locking and Unlocking Controls14 Troubleshooting15 Specifications16 Certifications19 PGXD1, PGXD2 20 PGXD4 20 LICENSING INFORMATION 20 Information to the user 20 Australia Warning for Wireless 20PGXD Wireless System Quick Start•••General DescriptionOffering uniquely tailored wireless solutions for vocalists, guitarists, and presenters, PGX-Digital combines the trusted legacy of Shure wired microphones with state-of-the-art, 24-bit digital wireless technology to deliver strong, clean RF performance. The result is wireless audio that sounds like wired, rock-steady RF signal even at extended distances, and plug-and-play setup and operation.Professional quality 24-bit digital audioDigital RF technology for rock-solid performanceWide variety of rugged and dependable Shure microphones•••••••••••••••One-touch setup and operationUp to 10 hours of battery life (9 hours, PGXD2)Up to 200 foot range (line-of-sight)900 MHz operation—free from white spaces or DTV interferenceSystem ComponentsAll systems includePGXD4 receiver2 AA alkaline batteriesPower supplyUser guideVocalist systems includePGXD2 handheld transmitterMicrophone head (choice of PG58, SM58, SM86, Beta 58A or Beta 87A*)Available only in select marketsMicrophone clipLavalier, Headworn, and Instrument systems includePGXD1 bodypack transmitterMicrophone (choice of WL93, WL185, PGA31 or Beta 98H/C)Guitar systems includePGXD1 bodypack transmitter1/4” to mini 4-pin guitar cable (WA302)®®1. 2. 3. 4.◦◦5. 6. 7. 8. 9. 10.Receiver Controls and ConnectorsFront Panelaudio LED: Indicates strength of incoming audio signal: green for normal, amber for strong and red for clipping.LED Screen: Displays group and channel setting. See "Single System Setup" for details.channel button: Changes group and channel setting. See "Single System Setup" for details.ready LED: Indicates system ready and receiving an RF signal from the transmitter. Also indicates battery level of the transmitter:Green = transmitter battery level normalRed = low battery (typically less than 60 minutes with alkaline batteries)with NiMH rechargeable batteries, when the indicator turns red there will be little to no remaining life.Infrared (IR) port: Sends IR signal to transmitter for sync.sync button: Press to synchronize transmitter with receiver group and channel settings.AC adapter jackAdapter cord tie-offXLR microphone output jack6.35 mm (¼") instrument level output jackBack PanelTransmitter Controls and Connectors① Indicator LED: Displays battery level, mute, and IR transmission status (see table).② Power/Mute Switch: Press to mute or unmute. Press and hold to power on or off.③ Infrared (IR) port: Receives infrared beam to synchronize frequencies. When using multiple systems, only one transmitter IR port should be exposed at a time.④ 4-Pin Microphone Input Jack⑤ Audio Gain Adjustment••••Transmitter Indicator LEDLED Indicator Status GreenReady Flashing green Controls locked Amber Mute onSolid red Battery power lowFlashing red Batteries dead (change batteries to power on transmitter)Rapidly flashing red IR transmission in process Flashing amber and redBattery power low and mute onBatteriesA fresh set of alkaline batteries lasts up to 10 hours (9 hours, PGXD2).The transmitter LED and the receiver ready LED glow red to indicate low battery (typically less than 60 minutes remain-ing).NiMH rechargeable batteries may be used. However, the low battery indicator functions differently. When it turns red, there may be little to no remaining life.When the LED flashes red, the batteries must be replaced to power on the transmitter.Wearing the Bodypack TransmitterClip the transmitter to a belt or slide a guitar strap through the transmitter clip as shown.For best results, the belt should be pressed against the base of the clip.••Adjusting LevelsAdjusting GainFor best audio quality, set transmitter gain so the audio LED on the receiver flickers green and amber during the performance. Decrease gain if the signal clips (LED turns red).The amber LED may appear to be red when viewed at an angle. For best results, monitor the LED from directly in front.Bodypack TransmitterThe bodypack has 26 dB of audio gain adjustment.Increase gain (clockwise +) for microphonesDecrease gain (counterclockwise –) for guitars or high-output instruments••1.1.Handheld TransmitterAccess the gain switch by unscrewing the head of the e the tip of a pen or a small screwdriver to move the switch.0dB: For quiet to normal vocal performance.-10dB:Use only if audio is distorted due to high vocal levels.Single System SetupScan: Use the scan feature on the receiver to find a clear channel.LED screendisplays current channel2.3. 2.1.2.3.4.5.◦◦Press channel button to scan for a clear channelSystem scans for the channel with the least interferenceSynchronize: Synchronize the transmitter to the receiver by aligning the infrared (IR) ports and pressing the sync but-ton. Make sure the IR ports are closely aligned. After a successful sync, the transmitter LED momentarily flashes red and the receiver readylight illuminates.This wireless system may be affected by RF interference when used in proximity to other wireless devices such as cordless phones, baby monitors, and two-way radios. For best results, use the scan and sync feature before each use or if you experience problems.Multiple System SetupUse the following steps to ensure the best performance when installing multiple wireless systems at the same location.Turn all receivers on and all transmitters off.Turn on any other digital equipment that could cause interference during the performance so it will be detected during the frequency scans in the following steps.Make sure the group number is the same for all receivers (see Manual Group Selection).Perform a scan using the first receiver.Turn on the first transmitter and sync it to the receiver.Repeat for each system.After syncing each transmitter, leave it on so that scans from the other receivers will not select that channel.Be sure only one transmitter IR port is exposed when synchronizing each system.patible FrequenciesWhen operating multiple systems simultaneously, choose one group from the following table and set all systems to different channels within that group.Number of SystemsGroup (by band)X8X8A (Australia)X8B (Brazil)20 to 90 to 90 to 93 3 to 9 3 to 9 3 to 94 3 to 9-- 3 to 957 to 9----Manual Channel and Group Selection (receiver only)Using the receiver to scan for a channel is the best way to find the best frequency for your system. However, for multiple sys-tem setup, you may need to manually set the group number.Press and hold the channel button.Hold the button until the channel or group display begins flashing.Release and press the button again to advance the setting. At the desired channel or group number, wait for the flash-ing to stop. This activates the new setting.Transfer the new frequency setting to the transmitter using the automatic sync function.Locking and Unlocking ControlsLocking the system controls prevents accidental muting or channel adjustment during performances.To lock the controls: With the transmitter off , hold the power button down until the green LED flashes (~5 seconds)To unlock the controls: With the transmitter on , hold the power button down until the green LED flashes (~5 seconds)To lock the channel: Hold the channel button until the numbers flash (~10 seconds)To unlock the channel: Hold the channel button until the numbers flash (~5 seconds)TroubleshootingIssue Indicator Status SolutionNo sound or faint sound Receiver ready LED on Verify all sound system connections Receiver ready LED offTurn on transmitterMake sure the batteries are installedcorrectlyPerform automatic transmitter setupInsert fresh batteriesReceiver LED screen offMake sure AC adapter is securelyplugged into electrical outlet Transmitter indicator LED flashingredReplace transmitter batteriesLED screen shows dash and valueError code displayed. Contact yourShure reseller for assistance.Transmitter LED flashes red for 7seconds after attempting to syncTransmitter and receiver incompatible.Contact your Shure reseller for assis-tance.Noise bursts or audio dropouts N/A Change receiver and transmitter to a different channelRemove nearby sources of RF interfer-ence such as cordless or cell phones, computers, wireless routers, media players, digital signal processors, and security systems.Replace transmitter batteriesIf using multiple systems, change the frequency of one of the active systemsDistortion Audio LED on receiver indicates clip-ping (red)Reduce transmitter gainDistortion increases gradually over time Transmitter power light glowing orflashing redReplace transmitter batteriesSound level different from cabledguitar or microphone or when usingdifferent guitarsN/A Adjust transmitter gain as necessaryIssue Indicator StatusSolutionCannot turn transmitter onTransmitter LED slowly flashing redReplace transmitter batteries Transmitter LED rapidly flashing redContact your Shure reseller for assis-tanceSpecificationsWorking RangeLine of Sight60m (200 ft)[1]RF Carrier RangeX8902–928 MHz X8A 915–928 MHzX8B902–907.5 MHz, 915–928 MHzAudio Frequency Response20 to 20,000 HzSystem GainPGXD16.35 mmConnector0 dBat minimum gain settingXLR output-12.5 dBat minimum gain settingPGXD2XLR output: -54.5 dBV/Paat –10 dB gain setting Total Harmonic Distortion<0.02%, A-weighted, typicalDynamic Range>108 dB, A-weightedOperating Temperature Range-18°C (0°F) to +50°C (122°F)Transmitter Audio PolarityPositive pressure on microphone diaphragm produces positive voltage on pin 2 with respect to pin 3 of low-impedance output and the tip of the high impedance 1/4-inch output[1] Actual range depends on RF signal absorption, reflection and interference.PGXD1Audio Input Levelat minimum gain setting+10 dBVmaximumat maximum gain setting-16 dBVmaximumGain Adjustment Range26 dBInput Impedance1 MΩRF Output Power10 mWPin Assignments1ground (cable shield)2+ 5 V Bias3audio4On instrument adapter cable, pin 4 floatsDimensions108 mm x 64 mm x 19 mmWeight128 g (4.5 oz.) (without batteries)HousingMolded Polycarbonate CasePower Requirements2 AA size alkaline or rechargeable batteriesBattery Lifeup to 10 hoursPGXD2Audio Input Levelat minimum gain setting+10 dBVmaximum at maximum gain setting-16 dBVmaximumGain Adjustment Range26 dBInput Impedance1 MΩRF Output Power10 mWPin Assignments1ground (cable shield)2+ 5 V Bias3audio4On instrument adapter cable, pin 4 floatsDimensions108 mm x 64 mm x 19 mmWeight128 g (4.5 oz.) (without batteries)HousingMolded Polycarbonate CasePower Requirements2 AA size alkaline or rechargeable batteriesBattery Lifeup to 10 hoursPGXD4Dimensions40 mm x 181 mm x 104 mmWeight289 g (10.2 oz.)HousingABSSensitivity-5-102 dBmat 10 BERPower Requirements12-18 V DC at 150 mA, supplied by external power supply (tip positive) Audio LEDRed2 Amber12 Green50 ConfigurationImpedance BalancedMaximum Audio Output LevelXLR connector-2.5 dBV6.35 mmConnector+10 dBV ImpedanceXLR50 Ω6.35 mmConnector50 ΩPin AssignmentsXLR1=ground, 2=audio, 3=no audio6.35 mm (1/4") TRS Tip=audio, Ring=no audio, Sleeve=groundXLR Output¼" Output1.2.••••CertificationsThis Class B digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.PGXD1, PGXD2Certified under FCC Part 15. (FCC ID: DD4PGXD1, DD4PGXD2).PGXD4Approved under the Declaration of Conformity (DoC) provision of FCC Part 15.LICENSING INFORMATIONLicensing: A ministerial license to operate this equipment may be required in certain areas. Consult your national authority for possible requirements. Changes or modifications not expressly approved by Shure Incorporated could void your authority to operate the equipment. Licensing of Shure wireless microphone equipment is the user’s responsibility, and licensability de-pends on the user’s classification and application, and on the selected frequency. Shure strongly urges the user to contact the appropriate telecommunications authority concerning proper licensing, and before choosing and ordering frequencies.Information to the userThis device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions:This device may not cause harmful interference.This device must accept any interference received, including interference that may cause undesired operation.Note: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installa-tion. This equipment generates uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:Reorient or relocate the receiving antenna.Increase the separation between the equipment and the receiver.Connect the equipment to an outlet on a circuit different from that to which the receiver is connected.Consult the dealer or an experienced radio/TV technician for help.Note: EMC conformance testing is based on the use of supplied and recommended cable types. The use of other cable types may degrade EMC perfor-mance.Changes or modifications not expressly approved by the manufacturer could void the user’s authority to operate the equipment.Australia Warning for WirelessThis device operates under an ACMA class licence and must comply with all the conditions of that licence including operating frequencies. Before 31 December 2014, this device will comply if it is operated in the 520-820 MHz frequency band. WARNING: After 31 December 2014, in order to comply, this device must not be operated in the 694-820 MHz band.。
XELSIS 产品说明书
Cod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47345678POS POS CODE CODE DESCRIPTION DESCRIPTIONNOTENOTE12NC 12NC 01 15002162 996530009411 MANUAL V2 SAE SUP038 MYB9/H D/I 15002163 996530009412 MANUAL V2 SAE SUP038 MYB9/H GB/F 15002164 996530009413 MANUAL V2 SAE SUP038 MYB9/H E/P 15002165 996530009414 MANUAL V2 SAE SUP038 MYB9/H NL/PL 15002166 996530066721 MANUAL V2 SAE SUP038 MYB9/H CHN 15001810 996530009297 MANUAL SAE SUP038Z MYB9/T D/I ID 15001811 996530009298 MANUAL SAE SUP038Z MYB9/T GB/F ID 15001812 996530009299 MANUAL SAE SUP038Z MYB9/T E/PID 15001813 996530009301 MANUAL SAE SUP038Z MYB9/T NL/PL ID15002167 996530068484 MANUAL V2 SAE SUP038 MYB9/H BRA GB 15002723 996530068485 MANUAL V2 SAE SUP038 MYB9/H PT/BRA 02 146680850 996530016529 BLACK COFFEE MEASURE SMART 03 127390100 996530011867 BRUSH FOR CLEANING04 11028379 996530070318 PSA KLUBER TRIBOSTAR SIL 2 TUBE 5GR.05 21002051 996530071783 A CC PSA MAVEA WATER FILTER 1UNIT USA-BRA06 0301.961 996530039604 STICKER FOR WATER HARDNESS TEST 07 183911050 996530025808 BLACK POWER CABLE SCHUKO L=1200 183930650 996530025858 POWER CABLE CH BLACK 3X1 H05VV-F L=1200 183921950 996530025843 POWER CABLE SJT3 USA BLACK UL L=1200 183940650 996530025872 BLACK POWER CABLE A USTRALIA L=1200 11001985 996530001089 BLACK POWER CABLE KOREA L=1200 11004246 996530002166 BLACK POWER CABLE TAIWAN HVCTF L=1200 11004247 996530002167 BLACK POWER CABLE RVV 3X1 CHINA L=1200 17800024 996530009632 BLACK POWER CABLE JAPPON VCTF L=1200MM 17800019 996530009627 BLACK POWER CABLE BRAZIL L=1200 220V 17800330 996530069926 BLACK POWER CABLE BRAZIL L=1200 127V08 13001097 996530008404 BAND FOR LIFTING L=1800MM H=300MM 09 20006891 996530067538 KIT PS-E INSERT FOR BOX MYB910 11022804 996530007446 SM/SS FRONT COVER CASING INS.MYB9/T A SSY ID 11 13000920 996530008348 DISPLAY BOX MYB9/H SAE XELSIS SS 13001110 996530008408 DISPLAY BOX MYB9/H SAE XELSIS CLASS 13001112 996530008411 DISPLAY BOX MYB9/T SAE XELSIS ID SS ID12 12000450 996530007819 SCREW TCBR TORX 10 3,5X12 PLAST.ZN-N 13 11013210996530006691 SB/SS G ROUND C ONT .DOOR C OVER S /S.MYB9 A S UP TO S/N. TU90122710414011025978996530072657 SB/SS G ROUND C ONT .DOOR C OVER S /S.MYB9 A S FROM S/N TU90122710414111023203996530007597 SM/SS G ROUND C ONT .DOOR C OVER S /S.MYB9 A S ID14 11013148996530006653 SB/SS W A TER C ONT AIN.DOOR C OVER M YB9 A SSYUP TO S/N. TU90122710414011029403996530072665 SB/SS W A TER C ONT .DOOR C OVER V 2 M YB9 A SSYFROM S/N TU90122710414111029406 996530072983 BLACK W A TER C ONT .DOOR C OVER V 2 M YB9 A SSY 11029404 996530072937 WHITE W A TER C ONT .DOOR C OVER V 2 M YB9 A SSY11023196 996530007595 SM/SS W A TER C ONT AIN.DOOR C OVER M YB9 A SSY ID 15 17000101996530070341 SB/SS LEFT SIDE PANEL V2 MYB9/H-T A SSY .UP TO S/N. TU90122710414011028790996530072664 SB/SS LEFT SIDE PANEL V2 MYB9/H-T A SSY .FROM S/N TU90122710414111024524 996530070337 BLACK LEFT SIDE PANEL V2 MYB9/H-T A SSY .11024523 996530070334 WHITE LEFT SIDE PANEL V2 MYB9/H-T A SSY . 11024522 996530070338 SM/SS LEFT SIDE PANEL V2 MYB9/H-T A SSY . ID16 11022426 996530007365 SS S PRING F OR W A TER C ONT AINER L EVER M YB917 12001263 996530008019 BLACK PUSH-PUSH MYB918 11022425 996530007364 YELLOW L EVER F OR W A TER C ONT AIN.S/SC.MYB919 11012859996530006558 SB/SS CUP WARMING LID MYB9UP TO S/N. 9010SA4002675817000071996530066728 SB/SS CUP WARMING LID MYB9 A SSY .FROM S /N.9010SA40026759 T O S /N. 9010SA40120165 17000059996530067839 SB/SS CUP WARMING LID V2 MYB9FROM S /N 9010SA40120165 T O S /N. T U901227104140 11025965996530072656 SB/SS CUP WARMING LID V2 MYB9FROM S/N TU90122710414120007063996530071497 KIT B LK C UP W ARMING+CASING C OVER V 2 M YB9 IDUP TO S/N 9010SA4012016511024245996530070333 SM/SS CUP WARMING LID V2 MYB9IDFROM S/N 9010SA401201662011013149996530006654 SB/SS B EAN C ONTAIN.DOOR C OVER M YB9 A SSY .UP TO S/N. TU90122710414011029385996530072661 SB/SS BEAN CONT .DOOR COVER V2 MYB9 A SSY .FROM S/N TU90122710414111029388 996530072981 BLACK B EAN C ONT .DOOR C OVER V 2 M YB9 A SSY . 11029386 996530072982 WHITE B EAN C ONT .DOOR C OVER V 2 M YB9 A SSY . 11023198 996530007596 SM/SS B EAN C ONTAIN.DOOR C OVER M YB9 A SSY . ID 2112001330996530008026TRANSP .ROUND A DHESIVE FOOT SJ-5382Cod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47345678POS POSCODE CODEDESCRIPTIONDESCRIPTIONNOTENOTE 12NC 12NC22 11022809996530007452 SB/SS R EAR C OVER C ASING I NSERT M YB9 A SSY UP TO S/N. TU901227104140 11025964996530072663 SB/SS R EAR C OVER C ASING I NSERT M YB9 A SSYFROM S/N TU90122710414111022821 996530007454 BLK REAR COVER CASING INSERT MYB9 A SSY . 11022820 996530007453 WHT REAR COVER CASING INSERT MYB9 A SSY . 11023105 996530007582 SM/SS R EAR C OVER C ASING I NSERT M YB9 A SSY ID23 17000205 996530066735 BLACK C OVER C ASING W /PROTECT .V2 M YB9/H-T 24 11003232 996530001522 A S.CPU+SW WATER LEVEL SENSOR V2 25 11014017 996530006969 BLACK H ORIZONT AL M OUNTING P LA TE M YB9 A SS 26 11024263 996530067506 PWR BOARD MDS 230V230V 11021342 996530007079 PWR BOARD MYB9/MDS 120V120V27 11012035 996530006299 BLACK PWR BOARD COVER MYB928 17000123 996530067922 BLACK R EAR P ANEL W HIT L OGO S AECO V 2 M YB929 11023304 996530007613 PWR ELECTR.BOARD INSULATE SHEET MYB9 ONLY 120V 30 17000102996530070339 SB/SS RIGHT SIDE PANEL V2 MYB9/H-T A SSY . UP TO S/N. TU90122710414011028791996530072662 SB/SS RIGHT SIDE PANEL V2 MYB9/H-T A SSY . FROM S/N TU90122710414111024520 996530070336 BLACK RIGHT SIDE PANEL V2 MYB9/H-T A SSY . 11024509 996530070335 WHITE RIGHT SIDE PANEL V2 MYB9/H-T A SSY . 11024508 996530070231 SM/SS RIGHT SIDE PANEL V2 MYB9/H-T A SSY . ID31 11022805996530007447 SB/SS FRONT COVER CASING INS.MYB9/H-P A S UP TO S/N. TU90122710414011025962996530072659 SB/SS FRONT COVER CASING INS.MYB9/H-P A S FROM S/N TU90122710414111022807 996530007449 BLK FRONT COVER CASING INS.MYB9/H-P A SSY 11022806 996530007448 WHT FRONT COVER CASING INS.MYB9/H-P A SSY 32 11013170 996530006676 BLACK STEEL CASING MYB9 A SSY .33 11021769 996530007136 BLACK RIGID FOOT FIXING PIN MYB934 11021829 996530007156 BLACK/TRANSP .RIGID FOOT MYB935 11022527 996530007397 BLACK DUMP BOX PROTECTION MYB936 11013595 996530006815 BLACK DUMP BOX MYB9 A SSY .37 11022169 996530007247 BLK DRIP TRAY COFF.BREW UNIT COVER MYB938 17001901 996530071992 BLACK COFFEE UNIT DRIP TRAY V2 MYB939 11013128 996530006642 40POLES FLAT CABLE BOARDS LINK MYB9 UL 4011013806996530006864 BLACK FLAT-CABLE PROTECTION MYB941 W GA 4316031000 996530063553 A DHESIVE HEATING-ELEMENT 230V 230V 11000791 996530000503 A DHESIVE HEATING-ELEMENT 120V100V-120V42 11008844 996530005056 2POLES C ONNECT .SUPPL Y C UPWARMING G 0053UL43 17000007 996530066726 COMPONENTS SUPPORT INSULATE SHEET MYB9 ONLY 120V 44 13001231 996530008429 PS-E INSERT FOR BOX MYB945 17000420 996530067881 BLACK SLIM BOILER PROTECTION MYB9 WITH B OILER S LIM4612001728996530070321 SCREW TCBR TORX 10 3,5X12 PLAST.SSCod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47POS POS CODE CODE DESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC 01 11022728996530007422 SB/SS FRONTAL PANEL MYB9 A SSY .UP TO S/N.9009SA4029891611023318996530007614 SB/SS FRONT PANEL V2 MYB9 A SSY .FROM S /N.9009SA40298917 T O S /N. T U901227104140 11025938996530072976 SB/SS FRONT PANEL V2 N/S/SCR.MYB9 A SSY .FROM S/N TU90122710414111013277996530006719 BLACK FRONT PANEL N/S/SCREEN MYB9 A SSY .UP TO S/N.9009SA4029891611023522996530007634 BLK FRONTAL PANEL V2 S/SCREEN MYB9 A SSY .FROM S/N.9009SA4029891711013298996530006732 WHITE FRONT PANEL N/S/SCREEN MYB9 A SSY .UP TO S/N.9009SA4029891611023523996530007635 WHT FRONTAL PANEL V2 S/SCREEN MYB9 A SSY .FROM S/N.9009SA4029891711023319 996530007615 SM/SS FRONTAL PANEL V2 MYB9 A SSY . ID02 11011406 996530006054 ADHES.SILVER PLATE LOGO SAECO P005703 11022725 996530007418 ADHES.SILVER PLATE LOGO XELSIS04 11021449996530007103 CHROM.CONNECT .CAP F OR C ARAFE M YB9 A SSY .UP TO S/N.9009SA4029891611024247996530066715 CHROM.CONNEC.CAP F OR C ARAFE V 2 M YB9 A SSYFROM S/N.9009SA4029891705 12001369 996530008039 SCREW TSP TORX 10 5X35 PLAST.SS06 17000215 996530070073 BLACK DRIP T RAY SUPPORT V2 MYB9/H-P A SSY 17000223 996530070223 BLACK DRIP TRAY SUPPORT V2 MYB9/T A SSY .07 11013143996530006649 CHROM.ZAMA FRONT FRAME MYB9UP TO S/N.9009SA4029891608 11001624 996530000929 BLACK STEAM TUBE WASHER P004909 NF11.084996530059199 PHOSPH/STEEL FOLK SPRINGUP TO S/N.9010SA4003648010 9011.144 996530048885 SS FORK FOR TUBE D=411 140328059996530013564 OR 2015 SILICONE PTFE/FDAUP TO S/N.9009SA4029891612 11013244 996530006708 TF/CHROM.- CHROM.STEAM TUBE MYB9 A SSY .13 11013215 996530006694 DOOR UPPER HINGE MYB914 11022860996530007469 BLACK CONNECTOR V2 FOR CARAFE MYB9UP TO S/N.9009SA402989161512001430996530008051SCREW TCBR TORX 20 4X10 PLAST.SS16 129573221 996530012922 SCREW TCB PH/TT 4X8 UNI 8112 SS17 11022870996530007476 STEEL S PRING F OR C ARAFE C ONNECTOR M YB9UP TO S/N.9009SA4029891618 11022853996530007466 BRASS I NSERT F OR S PRING C ARAFE C ONN.MYB9UP TO S/N.9009SA4029891619 12000450 996530007819 SCREW TCBR TORX 10 3,5X12 PLAST.ZN-N 20 11014015996530006967 BLK C ARAFFE B OARD S UPPORT G EN.MYB9 A SSY .UP TO S/N.9009SA4029891621 12001431 996530008052 SCREW TCB TORX 10 3X12 PLAST.SS22 11013784 996530006855 BLACK TEFLON TUBES SUPPORT COVER MYB923 11013785 996530006856 STEEL T EFLON T UBES SUPPORT SPRING MYB924 11021692996530007111 PREFORMED T EFLON T UBES S UPPORT M YB9 A SSYUP TO S/N.9009SA4029891625 11013216 996530006695 DOOR LOWER HINGE MYB926 20006820 996530010248 KIT SPARES FRONT PLATE DISPLAY MYB9/H-T 27 12001261 996530008018 DISPLAY 320x240 N O T OUCH B RIGHTNESS+MYB928 11013226 996530006702 BLACK DISPLAY SEAL MYB9/H-T 29 11012889 996530006568 BLACK DISPLAY SUPPORT MYB9/H-T 30 129913202 996530013156 SCREW TCB TORX 10 3X8 PLAST.ZN-B 31 149450588 996530018311 65SH SILICONE BOILER SEALING VALVE 32 11012377996530006381 BLACK CPU BOARD COVER MYB9/H-TUP TO S/N.9009SA4029891617000014996530009564 BLACK CPU BOARD COVER V2 MYB9/H-TFROM S/N.9009SA4029891733 11014215 996530007042 BLACK BUTTON FOR DOOR RELEASE MYB934 11021976 996530007207 STEEL PLATE FOR DOOR CLOSURE MYB935 11024441996530067552 CPU+SW MYB9/H PSA A SSY .UP TO S/N TU901150186483 11026862996530070332 CPU+SW MYB9/H SLIM A SSY .FROM S/N TU901150186484 11024443996530067563 CPU+SW MYB9/T PSA A SSY .IDUP TO S/N TU901150186483 11026860996530070331 CPU+SW MYB9/T SLIM A SSY . IDFROM S/N TU90115018648436 11000651 996530000425 WATER LEVEL SENSOR BOARD P1243711001339996530000763TRANSP .INFRAREDS PLATE P0049Cod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47POS POS CODE CODE DESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC 38 11022583996530007412 BLK I NSIDE C OVER F RON.PANEL S /SCR.MYB9/HUP TO S/N.9009SA4029891617000021996530009568 BLK I NSIDE C OVER F RON.PANEL V 2 S /S.MYB9HFROM S/N.9009SA4029891739 129912521 996530013148 SCREW TCBR TORX 10 3,5X14 PLAST.SS 40 11014218 996530007043 BLACK C OVER B UTTON F OR D OOR R ELEASE M YB941 11021747 996530007128 SPRING FOR DOOR RELEASE BUTTON MYB942 128310402 996530012341 GALV.WASHER UNI 8842 A 443 11023393996530007625 CHROM.ZAMA FRONT FRAME V2 MYB9FROM S/N.9009SA4029891744 17000006996530009559 TWO W A Y T EFLON T UBES S UPPORT S PRING M YB9FROM S/N.9009SA4029891745 11023352996530066675 WHT P REFORM.TEFLON T UBES S UPP .MYB9 A SSY .FROM S/N.9009SA4029891746 11023251996530007606 BLACK FRONTAL PANEL INSERT MYB9FROM S/N.9009SA4029891747 11023423996530066676 BLK C ARAFFE B OARD S UPP .GEN.V2 M YB9 A SSY .FROM S/N.9009SA4029891748 12001330 996530008026 TRANSP .ROUND A DHESIVE FOOT SJ-538249 17000055996530072431 GALVAN/STEEL FOLK SPRING W/FASTONFROM S/N. 9010SA4003648150 11006740996530003793 BRONZE SPRING FOR FRONT CASING G0053FROM S/N. 9010SA4003648151 17001306996530070328 BLACK FINGER PRINT COVER V2 MYB9/T ID52PARTICULAR NOT A VAILABLEID (SEE P ART .N°59)53 17000074 996530066729 BLK L OWER F INGERPRINT S ENS.SEAL V2 M YB9/T ID 54 11013309 996530006739 BLACK FINGER PRINT SUPPORT MYB9/T 55 129913702 996530013162 SCREW TCB TORX10 2,5X8 PLAST.ZN-B 56 17001078996530070329 BLACK V 3 F INGERPRINT S ENSOR S EAL M YB9/T ID57PARTICULAR NOT A VAILABLEID (SEE P ART .N°59)58 17000022 996530009569 BLK I NSIDE C OVER F RON.PANEL V 2 S /S.MYB9T ID 59 20006871996530066745 KIT SPARES FINGERPRINT SENSOR MYB9/T IDWITH PART . N°31-51-52-53-54-55-56-5760 11023238 996530007602 AS.SUPP .SCHEDA CARAF.V4 MYB9 NER.61 11013368 996530006754 MILKCARAFFE BOARD V2 MYB962 17000002 996530009555 BLACK CARAFE MICRO INSERT V2 MYB96317000019996530009566SS CARAFE MICRO INSERT SPRING MYB964 11013328 996530006745 BLACK CARAFFE BOARD COVER MYB965 17000045 996530009578 BLK P RESENCE C ARAFE M ICRO I NSERT V 2 M YB966 12000895 996530007951 SCREW TSP TORX 10 3X10 PLAST.SS67110131279965300066412POLES C ONNECT .SENSOR R EED D OOR M YB9 U LCod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-4721POS POSCODE CODEDESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC01 11027228996530070234 CHROM.COFFEE DISPENSER V2 MYB9 A SSY . WITH PART . N° 02 - 03 - 04 - 05 - 06 - 0702 129570721 996530012907 SCREW TCB TORX 10 M3X8 A UTOFORMING SS 03 11013001 996530006579 BLACK REAR COVER COFFEE DISPENSER MYB904 11013003 996530006582 BLACK COFFEE DISPENSER INNER BODY MYB905 11013085 996530006614 WHITE COFFEE DISPENSER SLIDER MYB906 11013002 996530006581 BLACK C OF .DISPENSER E XT .MOBILE P ART M YB907 11013222996530006699 CHROM.FRONT C OFFEE D ISPENSER C OVER M YB9 UP TO S/N TU90112107104011027227996530070327 CHROM.FRONT C OFF .DISPENSER C OVER V 2 M YB9 FROM S/N TU90112107104108 11022128 996530007237 C HROM.CREAM A DJUSTMENT K NOB S /SCR.MYB909 126772518 996530011711 SS COFF .CREAM A DJUSTMENT SHAFT SPRING 10 11013324 996530006743 BLACK CREAM A DJUSTMENT KNOB RING MYB911 140326661 996530013546 OR ORM 0040-20 BLACK EPDM12 140640200 996530013621 WHITE COFFEE CREAM A DJUSTMENT SHAFT 13 11013323 996530006742 CHROM.COFFEE DISPENSER SUPPORT MYB914 12000897 996530007953 SCREW TCB TORX 10 3,5X20 PLAST.SS 15 11013072 996530006604 BLACK SEAL FOR COFFEE DISPENSER MYB916 11013013 996530006585 BLACK COFFEE DISPENSER TUBE MYB917 12000070 996530007732 OR ORM 0060-15 SILICONE18 12000450 996530007819 SCREW TCBR TORX 10 3,5X12 PLAST.ZN-N 19 149360200 996530018269 SILICONE TUBE 5X8 70SR IN ROLL20 11013364 996530006752 BLACK DISPENSER COFF .INLET CONNECT .MYB92111012926996530006575 BLACK COFFEE DISPENSER CONNECTOR MYB9Cod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47POS POS CODE CODE DESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC 01 11013212 996530006692 NAT/GREY WATER CONTAINER MYB9 A SSY .02 11012738 996530006509 BLACK HANDLE WATER CONTAINER LID MYB903 140328461 996530013571 OR ORM 0190-10 EPDM04 224640200 996530029115 WHITE WATER CONTAINER EXT.FILTER A SSY .05 140324362 996530013507 OR ORM 0060-30 SILICONE06 126764718 996530011626 SS WATER CONTAINER VALVE SPRING 07 147660562 996530016894 GREY PISTON FOR WATER CONTAINER VALVE 0811012736996530006507NAT/GREY WATER CONTAINER LID MYB909 0701.031.150 996530044417 GREY WATER VALVE GACO DIM 14 SEAL COVER 10145842900996530015823NBR WATER CONT.VALVE GACO DIM14 SEAL 11 0701.014.150 996530044404 GREY WATER VALVE GACO DIM 14 SEAL CONT .12 140320461 996530013446 OR ORM 0080-20 TERMOIL13 129535721 996530012898 SCREW TSP TORX 10 A B 3,5X9,5 SHEET SS 14 20006842996530066742 SPARES KIT BEAN COFFEE CONT .LID V2 MYB9UP TO S/N.9010SA4012016517000202996530066734 NAT/GREY BEAN COFF .CONT .LID V2 MYB9 A SSYFROM S/N.9010SA4012016615 149213059 996530018116 GREY CAP FOR KNOBS BEAN CONTAINER 16 129913902 996530013164 SCREW TCB TORX 10 3X12 PLAST.ZN-B 17 20006843996530066743 SPARES K IT B EAN C OFFEE C ONTAINER V 2 M YB9UP TO S/N.9010SA4012016517000194996530066733 BLACK BEAN CONTAINER V2 MYB9FROM S/N.9010SA4012016618 11012852 996530006551 BLACK GRIND.ADJUSTMENT KNOB MBY919 11022528 996530007398 GREY G RIND.ADJUSTMENT K NOB W ASHER M YB920 145897300 996530016123 BLK I NSERT G RIND.ADJUSTMENT K NOB S MART121 11022803 996530007445 SS DRIP TRAY GRATE V2 MYB9/H-P 11022385 996530007345 SS DRIP TRAY GRATE MYB9/TID22 17000147 996530066732 BLACK GRATE SEAL V3 MYB9/H-P 23 11022802 996530007444 BLACK DRIP TRAY COVER V2 MYB9/H-P 11022384 996530007344 BLACK DRIP TRAY COVER MYB9/TID24 11005388 996530002866 RED FLOAT FOR DRIP TRAY P124 17000001 996530009554 RED FLOAT FOR DRIP TRAY MDSID25 17800061 996530066741 5POLES C ONNECTOR M C S ENSORS V 2 M YB9 U L 26 11013214996530006693 SB/SS-BLACK DRIP TRAY MYB9/H-P A SSY .UP TO S/N. TU90122710414026 11025971996530072666 SB/SS-BLACK DRIP TRAY MYB9/H-P A SSY .FROM S/N TU90122710414111013281 996530006723 BLACK/BLACK DRIP TRAY MYB9/H-P A SSY . 11013300 996530006734 WHITE/BLACK DRIP TRAY MYB9/H-P A SSY .11022389996530007348SM/SS-BLACK DRIP TRAY MYB9/T A SSY .IDCod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47POS POSCODE CODEDESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC01 11012845996530006545 TRANSP/CHROM.GEN.CARAFE M YB9 S AE A SSY .UP TO S/N.9009SA4029891602 11021984996530007208 CHROM.CARAFE C APP .COVER S /SC.MYB9 A SSY .UP TO S/N.9009SA4029891603 11022498 996530007389 CHR.CARAFE U PPER D ISPEN.SUPP .V2 S /S.MYB904 11012812 996530006527 VENTURI TUBE FOR CAPPUCCINATORE MYB905 11012678 996530006463 BLACK CARAFE MILK DISPENSER TUBE MYB906 11013163 996530006668 BLACK UPPER TUBE CARAFE CONNECT .MYB907 11022496 996530007388 CHRO.CARAFE L OWER D ISPENSER S UPP .V2 M YB908 11012816 996530006531 BLACK UPPER COVER PART CARAFE MYB909 12000550 996530007838 SCREW TCB TORX 10 3X25 PLAST.SS 10 11021893 996530007192 BLACK L OWER C OVER P ART C ARAFE M YB9 A SSY .11 11013466 996530006784 CHROM.CARAFE REAR PART HANDLE MYB912 11023158 996530007588 CHROM.CARAFE F RONT P ART H ANDLE V 2 M YB913 149360200 996530018269 SILICONE TUBE 5X8 70SRIN ROLL14 129911721 996530013137 SCREW TSP TORX 4X16 PLAST STEEL 15 11013164 996530006669 BLACK LOWER TUBE CARAFE CONNECT .MYB916 129910921 996530013128 SCREW TCB TORX 10 3X10 SS 17 9161.111 996530050555 MAGNET FOR FLOAT SUPERAUT.18 11014016 996530006968 SS.INSERT FOR MAGNET MYB919 12000895 996530007951 SCREW TSP TORX 10 3X10 PLAST.SS 20 11024104 996530007705 TRANSP .CARAFE V3 S/SCREEN MYB921 11012677996530006462 BLACK C ARAFE C APPUCCINA TORE C ASING M YB9UP TO S/N.9009SA4029891622 11026779 996530067887 WHITE MILK TUBE CONNECTOR V2 MYB923 11012814 996530067584 SILICONE TUBE 3,5X6 L=140 MM24 11013765 996530006849 STEEL SPRING FOR CARAFE LEVER MYB925 11022522 996530007393 BLACK CAM FOR CARAFE LEVERS V2 MYB926 11013832 996530006873 CARAFE SPRING WITH INSERT MYB9 A SSY .27 17000131 996530067459 BLACK LEVER FOR CARAFE CAM V3 MYB928 17000004996530009557 BLK C ARAFE C APPUCCIN.CASING W /CAPS M YB9FROM S/N.9009SA4029891729 11023235996530007599 CHR.CARAFE C APP .COVER V 2 S /SC.MYB9 A SSY .FROM S/N.9009SA4029891730 11023236996530007601 TRANSP/CHROM.GEN.CARAFE V 3 M YB9 S AE A SSYFROM S/N.9009SA4029891711023077996530067527TRANSP/CHROM.GEN.CARAFE M YB9/MDS A SSY . ONLY BRASILCod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47POS POS CODE CODE DESCRIPTIONDESCRIPTIONNOTENOTE 12NC 12NC 01 11012205 996530006316 BLACK COMPONENTS SUPPORT MYB902 11012412 996530006399 BLACK SOCKET-SWITCH SUPPORT MYB9 11023406 996530007626 BLACK SOCKET-SWITCH SUPPORT MYB9 UL 03 12001645 996530069782 CUP SOCKET V2 EN 60320 C 1304 NE03.038 996530058836 BIPOLAR SWITCH FUSIT C1250AR05 12000450 996530007819 SCREW TCBR TORX 10 3,5X12 PLAST.ZN-N 06 12001212 996530008003 ULKA PUMP MF 230V-50HZ 230V-50HZ 12001346 996530008028 ULKA PUMP MF 120V-60HZ 120V-60HZ 12001348 996530008031 ULKA PUMP MF 220V-50/60HZ 220V-50/60HZ 12001347 996530008029 ULKA PUMP MF 100V-50/60HZ100V-50/60HZ07 11012194 996530006315 BLACK/SBR ULKA MF PUMP SUPPORT 08 11013116 996530006631 2POLES CONNECTOR STEAM PUMP MYB9 UL 09 11012193 996530006314 90° INLET CONNECTOR FOR PUMP ULKA MF 10 147920300 996530017351 TRANS.CONNECTOR D=8 A RT .80.26.047 KA.46111 JS01.022 996530057357 SILICONE TUBE 5X10 60SH IN ROLL IN ROLL 12 147920150 996530017348 90° INLET PUMP CONNECTOR 13 12000140 996530007753 ULKA PUMP EP5/S GW 230V-50HZ 230V-50HZ 12000142 996530007754 ULKA PUMP EAP5/S 120V-60HZ 120V-60HZ 12000182 996530007782 ULKA PUMP EP5/S GW 220V-60HZ220V-60HZ 12000130 996530007749 ULKA PUMP EP5/S GW 100V-50/60HZ 100V-50/60HZ14 11013076 996530006607 2POLES CONNECTOR COFFEE PUMP MYB9 UL 15 11022857 996530007468 BLACK PUMP SUPPORT GMYB 16 140321961 996530013471 O-RING 2031 TERMOIL17 11001054 996530000624 BLK PUMP OUTLET 90°CONNECTOR P0049/B 18 12000781 996530007887 TURBINE D=1,2 932-8521/90 A SSY .19 11013122 996530006635 6POLES CONNECTOR FLOWMETER MYB9 UL 20 11011341 996530006025 V4 TUB.BOILER 1300W 230V A SSY . 230V-1300W 11011342 996530006026 V4 TUB.BOILER 1300W 120V A SSY . 120V-1300W 11011346 996530006031 V4 TUB.BOILER 1100W 100V A SSY .100V -1100W21 11007907996530004626 TUB.BOILER V5 SIDE THERMOS.FIXING SPRINGUP TO S/N.9009SA4026565711010164996530005641 TUB.BOILER V6 SIDE THERMOS.FIXING SPRINGFROM S/N.9009SA4026565822 12001715 996530069758 SCREW T CB T ORX 20 M 4X8 A UTOFORMING Z N-B 23 190800100 996530026997 TEMPERATURE SENSOR NTC UL L=280MM 24 189428600996530026958 THERMOSTAT 175° 1NT-08L-5125UP TO S/N.9009SA40265657 12001034996530007974 THERMOSTAT ONE SHOT 175°FROM S/N.9009SA402656582512000892996530007947OETIKER CLAMP D=9,5 1670000426 16000380 996530009505 5X8,9 SILICONE TUBE IN ROLLIN ROLL27 11013034 996530006589 STEEL TUB.BOILER SUPPORT MYB928 11012214 996530006322 BLACK TUB.BOILERS SUPPORT MYB929 12001280 996530008024 SCREW TSP TORX 10 5X20 PLAST.ZN-B 30 20006558 996530010201 BLOWDOWN VALVE CASING MYB9 A SSY .31129914302 996530013168 SCREW TORX M4X10 TESTA D=8X1,5 GALV 32 11022428 996530007366 BLK COF .BOILER INSULATING COVER MYB9/H-T 33 184320200 996530025907 SPRING HOSE CLAMP D=9,5MM34 11012206 996530006317 BLACK BLOWDOWN VALVE SUPPORT MYB935 129913902 996530013164 SCREW TCB TORX 10 3X12 PLAST.ZN-B 36 11013711 996530006834 BLACK VALVE FIXING FLANGE MYB937 11013165 996530006671 DIFFUSER VALVE MYB9 A SSY .38 11013528 996530006802 BLACK DIFFUSER PROTECT .VALVE MYB9 A SSY .3911021841 996530007157 SOLENOID VALVE CONNECT .INSUL.PLATE MYB940 11021842 996530007158 SOLENOID V AL VE C ONNECT .INSUL.WASHER M YB94120000590 996530009859 BLOWDOWN VALVE COIL P0049 230V A SSY . 230V 20000680 996530009874 BLOWDOWN VALVE COIL P0049 120V A SSY . 120V 20000920 996530009914 BLOWDOWN VALVE COIL P0049 220V-60HZ A SSY 220V-60HZ20000681 996530009875 BLOWDOWN VALVE COIL P0049 100V A SSY . 100V42 11007565 996530004421 BLACK B LOWDOWN V AL VE C ONNECTOR V 2 P 004943 11005096 996530002744 SS F ORK S PRING F OR B LOWDOWN V ALVE P 004944 12000620 996530007856 OR ORM 0130-20 EPDM45 11021450 996530007104 TUB.BOILER STD C1 INSULATING46 144650300 996530015428 ASPIRATION FILTER GAUZE NET 500 (NEW)11006995996530003974 BLOWDOWN VALVE V2 MYB9 230V A SSY . 230VWITH PART . N°30-41-42-43-4411022038996530007215 BLOWDOWN VALVE V2 MYB9 120V A SSY . 120VWITH PART . N°30-41-42-43-4411021989996530007212 BLOWDOWN VALVE V2 MYB9 220V-60HZ A SSY . 220V-60HZWITH PART . N°30-41-42-43-4411022037996530066669 BLOWDOWN VALVE V2 MYB9 100V A SSY . 100VWITH PART . N°30-41-42-43-4411009776996530005509 TUB.BOILER STD C1 1300W 230V A SSY .WITH PART . N°20-21-22-23-2411009778996530005511 TUB.BOILER STD C1 1300W 120V A SSY .WITH PART . N°20-21-22-23-2411012032996530066667 TUB.BOILER STD C1 1100W 100V A SSWITH PART . N°20-21-22-23-24Cod.10003050-51-52-53-3250-51-52Cod.10003278-79-3314-3450-70-71Cod.10003501-3663-3730-31-32-33SUP038SUP038ZRI9943/11-21-41-43RI9944/01-04-41-43RI9946/01-47916171810POS POSCODE CODEDESCRIPTIONDESCRIPTIONNOTENOTE12NC 12NC01 11011205 996530005991 TEA ZAMA INCREASE SCREW GRINDER 02 149198250 996530017951 BLK U PPER C OFFEEGRINDER G RINDER S UPPORT 03 140328960 996530013577 OR ORM 0530-15 NBR FDA 04 146520100 996530016342 BAKED CLAY GRINDER 48X2805 149198350 996530017952 BLK L OWER C OFFEEGRINDER G RINDER S UPPORT 06 123030300 996530011183 A XIAL BEARING EKKO 342.3525207 140360500 996530013597 FELT RING 45,4X39,4X408 12000450 996530007819 SCREW TCBR TORX 10 3,5X12 PLAST.ZN-N 09 11026355 996530068049 BLK G ROUND C OFF .OUTLET C ONVEYOR V 4 A SSY .10 11030459 996530072511 GRY G ROUND C OFFEE C ONT .LOWER S EAL S MART111 11012856 996530006555 COFFEE G RINDER S OUNDPROOFING C OVER M YB912 226000300 996530029609 GEAR SX Z=38 FOR GRINDER MOTION A SSY .13 149214800 996530018139 MC V 3 M OD=1,25 W ORM S CREW C ENTRING C AP 14 129661702 996530012992 SCREW TCB TORX 3,5X30 PLAST.ZN-B 15 128381902 996530012375 STEEL FLAT WASHER DI=4,3 DE=14 SP=1,516 129574802 996530012928 SCREW TCB TORX 20 M4X30 ZN-B17 11000597 996530000376 COFFEE GRINDER SUPPORT SPRING P12418 128310402 996530012341 GALV.WASHER UNI 8842 A 419 11002873 996530001389 INSULATOR BUSH FOR GRINDER MOTOR P12420 149199050 996530017963 BLK S ENSOR S UPPORT F OR C OF .GRINDER M OTOR 11000513 996530000317 MOTOR COFFEEGRINDER V3.1 230V A SSY . 230V 11000511 996530000315 MOTOR COFFEEGRINDER V3.1 120V A SSY . 120V 11000512 996530000316 MOTOR COFFEEGRINDER V3.1 100V A SSY . 100V22 11013079 996530006611 2POLES C ONNECTOR G RINDER M OTOR M YB9 U L 23 11000194 996530000115 BLACK C OFFEEGRINDER M OTOR S UPPORT P 12424 181540900 996530022784 ELECTR.BOARD WITH SENSOR HAL506 SF-K 25 123030200 996530011182 RADIAL BEARING 61900.2RS126 128381800 996530012374 STEEL CRINKLE WASHER 21,8X14X0,2527 142905450 996530015258 BLK U PPER B ODY W ITH C OFFEE G ROUND O UTLET 28 149199850 996530017976 LOWER G EAR S UPPORT C OFFEEGRINDER S MART 29 126772918 996530011715 SPRING FOR A DJUSTMENT GRINDING GEAR 30 146002150 996530016264 COFFEE GRINDING A DJUSTMENT GEAR SMART 31 149199750 996530017975 UPPER G EAR S UPPORT C OFFEEGRINDER S MART 32 129914002 996530013165 SCREW TCB TORX 10 3,5X16 PLAST.ZN-B 33 17001921 996530072477 BLK WIRING FIX INSERT FOR GRINDER BOARD 11013147 996530006652 GEN.MOTOR C OFFEE G RINDER M YB9 230V A SSY . 230V 11022036 996530007214 GEN.MOTOR C OFFEE G RINDER M YB9 120V A SSY . 120V11022035996530066668 GENERAL MOTOR MC MYB9 100V A SSY .100V。
仕博达户外精英产品详细操作指导
新表处于关机状态，按任意键启动（除了背光灯键）1.0 介绍● 感谢购买本手表。
手表具有能测量和显示户外状况的电子传感器：天气预报、温度、气压、高度和罗盘方向。
● 为你的徒步旅行、野营或其它户外活动（特别是长时间的）提供必要信息。
● 同时手表也包括时间、闹钟、秒表、倒计时、测步计和双时区功能。
● 手表经过精心的设计和生产适合于户外活动。
为了充分利用这些功能，使用前建议遵循以下事项：● 使用手表前请仔细阅读说明书。
● 避免手表暴露在极端条件下。
● 避免用力使用或重压手表。
● 请不要自行打开手表的后盖，因为设备包含精密的电子传感器和元件。
● 经常用软布清洁设备以达到更长的寿命。
● 手表请远离含有磁性元件的磁铁或设备，如手机、扬声器或马达。
● 手表不使用时，请放置在干燥的地方。
2.0 按键介绍3.0 主要功能模式手表共有9大模式：时间模式、闹钟模式、秒表模式、倒计时模式、测步计模式、双时区模式、高度计模式、气压计模式、数字罗盘。
按模式键切换时间菜单，按传感器键切换传感器菜单，长按传感器键从传感器菜单切换到时间菜单。
如下图所示： 4.0 时间模式－功能显示 功能显示● 时间模式包括4种功能显示：星期、温度、高度变化趋势图、海平面气压变化趋势图。
● 首行：按键切换显示星期、温度、高度变化趋势图和海平面气压变化趋势图● 中间：时间● 底行：日期自动切换● 长按键激活自动切换功能，当长按住键，4种功能会逐个显示。
4.1时间模式－天气预报 天气预报● 预测未来天气是手表的一个特殊功能。
通过分析过去大气压变化来预测。
如何预测未来天气● 手表提供4种符号代表不同天气模式键（Mode ）切换时间、闹钟、秒表、倒计时、测步计和双时区模式在设定模式下选择设定项目传感键（Sensor ） 切换显示高度计、气压计和罗盘模式 开始/暂停键（Start/Stop) 在同一模式下，切换显示功能在秒表、倒计时和测步计模式下，按此键开始/暂停计时/测步 设置数字时的数值增加键； 切换Yes/No圈数/重置键（Lap/Reset ）在秒表模式下，按此键记录圈数或重置计时的功能； 将光标向左移动显示历史数据； 设置数字时的数值减少键；切换Yes/No 背光灯键（Light ）启动背光灯约3秒·晴天（Sunny ）·晴间多云（Cloudy with Sun ） ·多云（Cloudy ） ·下雨（Raining ）注意：由于手表通过大气压变化来预测未来天气，当使用者在同一个地方至少24小时所得的结果会更加准确。
载体介绍
pBR322—→插入在Ampr 中，基因型为Tetr 、Amps 、—— →在含有氨卞青霉素培养基上不生长，在含有四环素培 养基可生长； 而在两种抗生素培养基上都生长的是非重组型。
这种在一个基因位点中插入外源DNA片段，从而使该 基因活性丧失的现象叫插入失活。
外源基因的插入：
PstI Amp r Tet r Amp s Tet r
酵母人工染色体载体（yeast artificial chromosome, YAC）
YAC的特点： 1.只能在酵母细胞中扩增 2.克隆容量大，一般为200kb-500kb 3.构建原理，按染色体结构构建
染色体复制和遗传的三个基本组件：
1.自主复制序列（ARS） （autonomous replicating sequence） DNA复制起始点（ori） 2.着丝粒（centromere，cen） 3.端粒（telomeres，tel）
P1 噬菌体载体和 P1 人工染色体载体
P1 噬菌体 ：大肠杆菌的一种溶原性噬菌体。 P1 噬菌体载体 工作原理类似黏粒载体 外源片断70-100kb
P1 人工染色体载体 P1 噬菌体载体的改造，结合了P1 噬菌体载体和 BAC载体的优点。 克隆外源片断130-150kb
（详细介绍）质粒载体
什么是质粒？
1.“严紧型”复制控制的质粒(stringent plamid)：拷 贝数少（1-5个）； 2.“松弛型”复制控制的质粒(relaxed plasmid)：拷 贝数多（10-200个）。
质粒DNA的转移
1. 质粒的类型 据能否自我转移可分为:
接合型（conjugative plasmid)
非接合型（non-conjugative plasmid) 2. F质粒(fertility factor)