carello_chassis_1_2015
欧里森UHF扫描系统移动无线电手册说明书
LBI-38904BMaintenance ManualORION™UHFSCAN AND SYSTEMMOBILE RADIOTABLE OF CONTENTSSynthesizer/Receiver/Exciter . . . . . . . LBI-39033Power Amplifier . . . . . . . . . . . . . . LBI-39034PA Interface . . . . . . . . . . . . . . . . LBI-38994Control Logic/IF Board . . . . . . . . . . LBI-38907Control Units . . . . . . . . . . . . . . . LBI-38992Assemblies . . . . . . . . . . . . . . . . LBI-38909Service Section . . . . . . . . . . . . . . LBI-38908Ericsson Inc.Private Radio SystemsMountain View RoadLynchburg, Virginia 24502ericssonz1-800-528-7711 (Outside USA, 804-528-7711)Printed in U.S.A.Copyright© October 1993, Ericsson GE Mobile Communications Inc.SPECIFICATIONS*Frequency Range:403-440 MHz 440-470 MHz 470-512 MHzRegulatory ApprovalFCC (United States)AXATR-315-A2403-440 MHz 20/40 Watts AXATR-315-B2440-470 MHz 30/40 Watts AXATR-315-C2470-512 MHz 35 Watts AXATR-316-A2403-440 MHz 100 Watts AXATR-316-B2440-470 MHz 100 Watts AXATR-316-C2470-512 MHz 80 Watts DOC (Canada)TR-315TR-315403-440 MHz 20/40 Watts 440-470 MHz30/40 WattsBattery Drain:ReceiveSquelched 1.1 Amperes at 13.8 V oltsUnsquelched 3.0 Amperes at 13.8 V olts (15 Watts Output)Transmitter20 Watts 35/40 Watts 80/100 Watts12 Amperes at 13.2 V olts 14 Amperes at 13.6 V olts 25/28 Amperes at 13.4 V olts Frequency Stability:0.0002% depending on model Temperature Range:-30° C (-22° F) to +60° C (+140° F)Duty Cycle:100% Receive, 14% Transmit TransmitterTransmit Output Power:20W/35W/40W/80W/100W Conducted Spurious:-85 dB Modulation:±4.5 kHzAudio Sensitivity:55 to 110 millivoltsAudio Frequency Characteristics:Within +1 dB to -3 dB of a 6 dB/octave pre-emphasis 300 Hz and within +1 dB to -4.5 dB of a 6 dB/octave pre-emphasis 3000 Hz per EIA standards. Post-limiter filter per FCC and EIA.Distortion:Less than 2% (1000 Hz)Less than 5% (3000 Hz)Deviation Symmetry:0.3 kHz maximum Maximum Frequency Separation:403-440 MHz 37 MHz 440-470 MHz 30 MHz 470-512 MHz 42 MHz Microphone Load Impedance:600 OhmsPower Adjust Range:100% to 50% of rated power (U.S.A. Models)100% to 30% of rated power (Euro Models)RF Output Impedance:50 Ohms FM Noise:45 dBContinuedThis manual covers Ericsson and General Electric products manufactured and sold by Ericsson Inc.NOTICE!Repairs to this equipment should be made only by an authorized service technician or facility designated by the supplier.Any repairs, alterations or substitution of recommended parts made by the user to this equipment not approved by the manufacturer could void the user’s authority to operate the equipment in addition to the manufacturer’s warranty.NOTICE!This manual is published by Ericsson Inc., without any warranty. Improvements and changes to this manual necessitated by typographical errors, inaccuracies of current information, or improvements to programs and/or equipment, may be made by Ericsson Inc., at any time and without notice. Such changes will be incorporated into new editions of this manual. No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying and recording, for any purpose, without the express written permission of Ericsson Inc.The software contained in this device is copyrighted by the Ericsson Inc. Unpublished rights are reserved under the copyright laws of the United States.NOTICE!LBI-389041DESCRIPTIONThe synthesized ORION mobile radio combinations are completely solid-state, utilizing microcomputer technology and integrated circuits to provide high-quality, high-reliabil-ity radios. Standard combinations may be equipped with:•Microcomputer Controlled Frequency Synthesizer•Up to 16 Channels•0.0002% Frequency Stability•Other Structured OptionsThe basic radio consists of three printed wiring boards mounted in a cast aluminum frame. The three boards are:1.The Control Logic/IF board2.The Frequency Synthesizer/Receiver/Exciter board3.The Power Amplifier boardThe radio is of double-layer construction with tuning ad-justments easily accessible from the top of the radio.The Control Logic/IF Board is located on the top of theradio, while the Power Amplifier and the Synthesizer/Re-ceiver/Exciter boards are located on the bottom of the radio.SYNTHESIZER/INTERCONNECTThe synthesizer consists of a microcomputer, E lectricallyE rasable P rogrammable R ead O nly M emory (EEPROM), afrequency synthesizer IC, transmit and receive V oltage C on-trolled O scillator’s (VCO) and associated circuitry. The fre-quency synthesizer under control of the microcomputergenerates all transmit and receive Radio Frequencies (RF).The EEPROM stores binary data for all radio frequen-cies, Channel Guard tones/digital codes and the timing func-tion of the C arrier C ontrol T imer (CCT). Themicrocomputer accesses the EEPROM and provides the correctW ALSH bits to the Channel Guard circuitry to generate thecorrect Channel Guard tone or digital code on a per-channelbasis.PROGRAMMINGThe EEPROM allows the radio to be programmed or repro-grammed as needed to adapt to changing system requirements.Radio Frequencies, Channel Guard tone and digital codes andthe CCT function can be reprogrammed.The EEPROM can be reprogrammed through the radiofront connector using a personal computer. This programmerallows all information to be loaded simultaneously.Programming instructions are provided in the respectiveProgrammer Maintenance Manuals.TRANSMITTERThe transmitter consists of the exciter, frequency synthe-sizer, transmitter VCO and a Power Amplifier (PA) assembly.The PA assembly consists of a PA board mounted on a heatsink assembly. The PA board also contains an antenna switch-ing diode and a low-pass filter.Audio and Channel Guard circuitry for the transmitter is lo-cated on the Control Logic/IF Board.RECEIVERThe receiver consists of the frequency synthesizer, RXVCO, injection amplifiers, front end, IF and limiter detector.Audio, squelch and Channel Guard circuitry for the receiver islocated on the Control Logic/IF Board.SYSTEM CONTROL FUNCTIONA microprocessor on the Control Logic/IF board controlsthe frequency synthesizer, the TX ON/OFF, the decoding ofCTCSS tones, the generation of CTCSS tones,... etc. The audioprocessor circuitry of the transmitter and the receiver are lo-cated on the Control Logic/IF board. Squelch circuitry and aconnection to the digital AEGIS circuit is also located on theControl Logic/IF board.OPERATIONComplete operating instructions for the ORION Two-WayRadio are provided in Operator’s Manual LBI-38888 for thecontrol unit used.MAINTENANCEThe Service Section in maintenance manual LBI-38908contains the maintenance information to service this radio. TheService Section includes:•Dissassembly Procedures•Replacement of IC’s, chip capacitors and resistors•Alignment procedures for the transmitter and receiver•Troubleshooting Procedures and wave formsA mechanical layout for the radio is found in ORION As-semblies Maintenance Manual LBI-38909.Figure 1 - ORION Mobile RadioSPECIFICATIONS* - Cont.ReceiverAudio Output:15 Watts with less than 3% distortion(To 4.0 ohm speaker)Sensitivity:0.35 µV (STD)/0.22 µV (PRE)12 dB SINAD (IEIA method)Selectivity:-85 dB (STD)-80 dB (PRE)EIA Two-Signal Method(25 kHz Channels)Spurious Response:-100 dB (STD)/-90 (PRE)Intermodulation 25 kHz:-85 dB (STD)/-80 dB (PRE)Maximum Frequency Separation:403-440 MHz .... 37 MHz440-470 MHz .... 30 MHz470-512 MHz .... 42 MHzFrequency Response:Within +1, -3 dB of 6 dB/octave de-emphasis from 300 to 3000MHz (1000 Hz reference)RF Input Impedance:50 OhmsHum/Noise ratio:Unsquelched-50 dBSquelched-70 dBChannel Spacing:30 kHz*These specifications are intended primarily for use of the service technician. Refer to the appropriate Specifications Sheet for the complete specifications.LBI-389042SYSTEM INTERCONNECTION DIAGRAMLBI-38904 Array U.S.A. LOW POWER3SYSTEM INTERCONNECTION DIAGRAM LBI-38904U.S.A. HIGH POWER4SYSTEM INTERCONNECTION DIAGRAMLBI-38904 Array EUROPEAN5。
Logix5000控制器指令执行时间和内存使用参考手册说明书
Reference ManualOriginal InstructionsEstimated Execution Time and Memory Use for Logix5000 Controllers InstructionsCatalog NumbersThis publication provides estimated instruction execution times and memory use information for Logix5000™ controllers in RSLogix 5000® software and Studio 5000 Logix Designer® application projects.Controller/programming software compatibility varies based on controller family and catalog number. For information on compatibility, see the controller documentation.Summary of ChangesThis publication has been revised to add the IMPORTANT table on page 2 indicating that you need to open the PDF file in Adobe® Acrobat® instead of in a web browser.ControlLogix 55701756-L71, 1756-L72, 1756-L73, 1756-L74, 1756-L75GuardLogix 55701756-L72S, 1756-L73S ControlLogix 55601756-L61, 1756-L62, 1756-L63, 1756-L64, 1756-L65GuardLogix 55601756-L61S, 1756-L62S, 1756-L63S CompactLogix 53701769-L16ER-BB1B, 1769-L18ER-BB1B, 1769-L18ERM-BB1B1769-L24ER-QB1B, 1769-L24ER-QBFC1B, 1769-L27ERM-QBFC1B1769-L30ER, 1769-L30ERM, 1769-L30ER-NSE, 1769-L33ER, 1769-L33ERM, 1769-L36ERM1768 CompactLogix 1768-L43, 1768-L451768 Compact GuardLogix 1768-L43S, 1768-L45S1769 CompactLogix 1769-L23E-QB1B, 1769-L23E-QBFC1B, 1769-L23-QBFC1B, 1769-L31, 1769-L32C, 1769-L35CR, 1769-L32E, 1769-L35EDriveLogix 573020D PowerFlex 700S with DriveLogix Estimated instruction execution times are available for the following Logix 5000™ controllers:•ControlLogix® 5580 controllers •GuardLogix® 5580 controllers •CompactLogix™ 5380 controllers •Compact GuardLogix 5380 controllers For more information, see the Estimated Logix 5000 Controller Execution Times Reference Manual, publication LOGIX-RM002.2Rockwell Automation Publication 1756-RM087P-EN-P - July 2020Estimated Execution Time and Memory Use for Logix5000 Controllers Instructions Reference ManualPDF File AttachmentsMicrosoft® Excel® files are attached to this PDF file. The files list execution time and memory use data for Logix5000 controller instructions in RSLogix 5000 software or Logix Designer application projects.The following types of files are available:• A Microsoft Excel file that includes information for all controller families.• A Microsoft Excel files for individual controller families so you can focus on specific controller families.To use the attached files, click the Attachments link, that is, the paper clip icon, and double-click the desired file. IMPORTANT Download the PDF file to your computer and open it with Adobe Acrobat software.If you download the PDF file from Literature Library and open it locally on your computer, you can access, download, and use the Microsoft Excel files.You can open PDF files in some web browsers. However, not all web browsers provide the option to access and open attachments from a PDF file when displaying it.Estimated Execution Time and Memory Use for Logix5000 Controllers Instructions Reference Manual Studio 5000 EnvironmentThe Studio 5000® Engineering and Design Environment combines engineering and design elements into a common environment. The first element in the Studio 5000 environment is the Logix Designer application. The Logix Designer application is the rebranding of RSLogix 5000 software and is the product to program Logix5000 controllers for discrete, process, batch, motion, safety, and drive-based solutions.The Studio 5000 environment is the foundation for the future of Rockwell Automation® engineering design tools and capabilities. It is the oneplace for design engineers to develop all elements of their control system.Rockwell Automation Publication 1756-RM087P-EN-P - July 20203Publication 1756-RM087P-EN-P - July 2020 | Supersedes Publication 1756-RM087O-EN-P-January 2020Copyright © 2020 Rockwell Automation, Inc. All rights reserved. Printed in the U.S.A.Rockwell Otomasyon Ticaret A.Ş. Kar Plaza İş Merkezi E Blok Kat:6 34752 İçerenköy, İstanbul, Tel: +90 (216) 5698400 EEE Yönetmeliğine UygundurAllen-Bradley, CompactLogix, ControlLogix, DriveLogix, expanding human possibility, GuardLogix, Logix5000, Logix 5000, PowerFlex, Rockwell Automation, Rockwell Software, RSLogix 5000, Studio 5000, and Studio 5000 Logix Designer are trademarks of Rockwell Automation, Inc.Acrobat and Adobe are trademarks of Adobe Systems, Inc.Excel and Microsoft are trademarks of Microsoft Corporation.Trademarks not belonging to Rockwell Automation are property of their respective companies.Rockwell Automation maintains current product environmental compliance information on its website at rok.auto/pec .Rockwell Automation SupportUse these resources to access support information.Documentation FeedbackYour comments help us serve your documentation needs better. If you have any suggestions on how to improve our content, complete the form at rok.auto/docfeedback .Technical Support CenterFind help with how-to videos, FAQs, chat, user forums, and product notification updates.rok.auto/support KnowledgebaseAccess Knowledgebase articles.rok.auto/knowledgebase Local Technical Support Phone NumbersLocate the telephone number for your country.rok.auto/phonesupport Literature LibraryFind installation instructions, manuals, brochures, and technical data publications.rok.auto/literature Product Compatibility and Download Center (PCDC)Download firmware, associated files (such as AOP, EDS, and DTM), and access product release notes.rok.auto/pcdc。
RecSys Challenge 2015
RecSys Challenge 2015任务描述:在一个电子商务的网站上通过一些用户在Typical Sesslion期间的一系列点击事件去预测用户是否打算购买商品,如果他正在购买商品,那么他将会购买何种商品,因此该任务被分解成两个子任务:1:用户在会话期间是否打算购买商品。
2:若果购买,他打算购买何种商品。
任务流程:一、数据文件(Training Data Files)yoochoose-clicks.dat和yoochoose-buys.dat。
yoochoose-buys.dat该文件中的Session ID将一直存在于yoochoose-clicks.dat 中,并且他们都是源自于一个用户在会话期间的一系列的点击事件,会话时间可长可短,会话期间可能有一次甚至成千上百次的点击,所有的这些都取决于用户的行为。
二、测试文件(Test File)yoochoose-test.dat和yoochoose-clicks.dat具有相同的数据三、解决方案文件(Solution file)解决方案要解决任务描述的内容包括:预测Test File 文件中每个Session是否购买商品以及购买哪种商品,不必去预测购买商品的数量。
Solution file 的文件形式为(solution.dat)里必须包括两方面的内容1:Session ID 2:以逗号为间隔列出这次会话中所购买的商品的ID。
注意:所有在solution.dat中存在的Session ID 均来自于测试文件(Test File)并且只要是在solution.dat中出现的Session ID均是挑战者预测可以成为购买事件的会话(至少完成一次购买事件)。
商品ID之间用“,”间隔,Session ID和Item ID 之间用“;”间隔。
四、预测结果分析:如果一个Session ID在测试文件(Test File)中存在,但在solution.dat文件中不存在说明这次会话将不会成为购买事件。
Matrox Solios eCL XCL摄像头接口应用说明书
BASLER L304kc August 15, 2006Basics about thecameraCamera Descriptions Effectiveresolution:Upto4080pixels/**************************. Camera Link BASE interface (8 or 10-bits, dual tap, 8-bit RGB). External and internal sync. External or internal exposure control. 30 or 60 MHz pixel clock rate. Mode of operations asper Matrox Imaging (inparentheses as percamera manufacturer)Interface Mode Fixed line scan rate (EXSYNC, level-controlled*) Variable line scan rate (EXSYNC, level-controlled) Fixed line scan rate with frame trigger (EXSYNC, level-controlled*) Variable line scan rate with frame trigger (EXSYNC, level-controlled) Fixed line scan rate with variable frame size (EXSYNC, level-controlled*) Variable line scan rate with variable frame size (EXSYNC, level-controlled) Basics about theinterface modesCamera Interface Briefs Mode 1: Fixed line scan rate 4080 pixels/line. Camera Link BASE interface (8bit RGB). DCF configured for 1000 lines per virtual frame. Matrox Solios eCL/XCL sending periodic EXPOSURE1 (EXSYNC) signal to camera. Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) and videodata from camera. DCF used: L304kc_4080_8bitRGBFLS.DCF*or Free Run programmableBASLER L304kc August 15, 2006 Basics about theCamera Interface Briefs (cont.)interface modesMode 2: Variable line scan rate4080 pixels/line.Camera Link BASE interface (8bit RGB).DCF configured for 1000 lines per virtual frame.Matrox Solios eCL/XCL receiving external TTL line trigger signal.Matrox Solios eCL/XCL sending EXPOSURE1 (EXSYNC) signal to camera.Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) andvideo data from camera.DCF used: L304kc_4080_8bitRGBVLS.DCFMode 3: Fixed line scan rate with frame trigger4080 pixels/line.Camera Link BASE interface (8bit RGB).DCF configured for 1000 lines per virtual frame.Matrox Solios eCL/XCL receiving external TTL frame (virtual) trigger signal.Matrox Solios eCL/XCL sending periodic EXPOSURE1 (EXSYNC) signal tocamera.Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) andvideo data from camera.DCF used: L304kc_4080_8bitRGBFLSFT.DCFBASLER L304kc August 15, 2006 Basics about theCamera Interface Briefs (cont.)interface modesMode 4: Variable line scan rate with frame trigger4080 pixels/line.Camera Link BASE interface (8bit RGB).DCF configured for 1000 lines per virtual frame.Matrox Solios eCL/XCL receiving external TTL frame (virtual) and line triggersignals.Matrox Solios eCL/XCL sending EXPOSURE1 (EXSYNC) signal to camera.Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) andvideo data from camera.DCF used: L304kc_4080_8bitRGBVLSFT.DCFMode 5: Fixed line scan rate with variable frame size4080 pixels/line.Camera Link BASE interface (8bit RGB).DCF configured for 1000 lines per virtual frame.Matrox Solios eCL/XCL receiving external TTL frame (virtual) trigger signal.Matrox Solios eCL/XCL sending periodic EXPOSURE1 (EXSYNC) signal tocamera.Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) andvideo data from camera.DCF used: L304kc_4080_8bitRGBFLSVF.DCFBASLER L304kc August 15, 2006 Basics about theCamera Interface Briefs (cont.)interface modesMode 6: Variable line scan rate with variable frame size4080 pixels/line.Camera Link BASE interface (8bit RGB).DCF configured for 1000 lines per virtual frame.Matrox Solios eCL/XCL receiving external TTL frame (virtual) and line triggersignals.Matrox Solios eCL/XCL sending EXPOSURE1 (EXSYNC) signal to camera.Matrox Solios eCL/XCL receiving LVAL, PIXEL CLOCK (@ 30 MHz) andvideo data from camera.DCF used: L304kc_4080_8bitRGBVLSVF.DCFSpecifics about theCamera Interface Detailsinterface modesMode 1: Fixed line scan rateLine rate: The frequency of the periodic EXPOSURE1 (EXSYNC) signaldetermines the camera’s line rate. The maximum line rate for this cameraequals 3.7 kHz.Exposure time: For EXSYNC, level-controlled mode the exposure time isthe inactive (low level) period between the falling and rising edges of theEXPOSURE1 (EXSYNC) signal. The default exposure time for this DCF is139 μs. Maximum/minimum exposure time per line for this DCF is 559 msand 33.3 ns respectively. The exposure time can be modified in the DCFusing Matrox Intellicam or with the MIL MdigControl() function. Consult therespective manual for more information.Camera communication: This DCF will work with all Free Run andEXSYNC modes however EXSYNC, Level controlled is the recommendedmode for use with this DCF. Set the mode using the Basler CameraConfiguration Tool Plus (CCT+). Refer to the camera manual for additionalinformation.Parameter SettingVideo Data Output Mode 3 taps, 8-bitsExposure Time Control Mode EXSYNC, Level-controlledBASLER L304kc August 15, 2006 Specifics about theCamera Interface Details (cont.)interface modesMode 1: Fixed line scan rate (cont.)Timing diagram (level controlled mode from camera):Mode 2: Variable line scan rateLine rate: The line rate is controlled by the frequency of the external TTL linetrigger signal. The line trigger signal period must be larger than the totalduration of the exposure time (low level duration of the timer), the internaldelay of the camera (≈2.5 μs) and the line read out (136 μs).Exposure time: Refer to Mode 1: Fixed line scan rate.Camera communication: This DCF works with all EXSYNC modes howeverEXSYNC, Level-controlled is the recommended mode for use with thisDCF. Set the mode using the Basler Camera Configuration Tool Plus(CCT+). Refer to the camera manual for additional information.Parameter SettingVideo Data Output Mode 3 taps, 8-bitsExposure Time Control Mode EXSYNC, Level-controlledTiming diagram (level controlled mode from camera):BASLER L304kc August 15, 2006 Specifics about theCamera Interface Details (cont.)interface modesMode 3: Fixed line scan rate with frame triggerLine/frame rate: The line rate is fixed and controlled by the frequency ofEXPOSURE1 (EXSYNC) signal. The default exposure time for this DCF is130 μs. The virtual frame rate is variable and controlled by the period of theexternal frame trigger signal, however the external frame trigger period mustalways be greater than the total time of the number of lines captured. Thenumber of lines per virtual frame (maximum of 1000 for this DCF) is fixed andcontrolled by the vertical timing of the DCF. Capture of the lines will start withthe rising edge of the frame trigger signal.Exposure time: Refer to Mode 1: Fixed line scan rate.C amera communication: Refer to Mode 1: Fixed line scan rate.Timing diagram (level controlled mode from camera):Mode 4: Variable line scan rate with frame triggerLine/frame rate: The line rate is controlled by the frequency of the externalTTL line trigger signal. The line trigger signal period must be larger than thetotal duration of the exposure time (low level duration of the timer), theinternal delay of the camera (≈2.50 μs) and the line read out (136 μs). Thedefault exposure time for this DCF is 130 μs. The virtual frame rate isvariable and controlled by the period of the external frame trigger signal,however the external trigger period must always be greater than the totaltime of the number of lines captured. The number of lines per virtual frame(1000 for this DCF) is fixed and controlled by the vertical timing of the DCF.Capture of the lines will start with the rising edge of the frame trigger signal.Exposure time: Refer to Mode 1: Fixed line scan rate.Camera communication:Refer to Mode 2: Variable line scan rate.Continued.…BASLER L304kc August 15, 2006 Specifics about theCamera Interface Details (cont.)interface modesMode 4: Variable line scan rate with frame triggerTiming diagram:Mode 5: Fixed line scan rate with variable frame sizeLine/frame rate: The line rate is fixed and controlled by the frequency ofEXPOSURE1 (EXSYNC) signal. The default exposure time for this DCF is130 μs. The number of lines per virtual frame (maximum of 1000 for thisDCF) is variable and controlled by the frame trigger signal. Matrox SolioseCL/XCL captures lines during the high level of the frame trigger signal. Tomodify the maximum amount of lines captured, change the active verticaltiming period in the DCF. Capture of the lines will start with the rising edge ofthe frame trigger signal.Exposure time: Refer to Mode 1: Fixed line scan rate.Camera communication:Refer to Mode 1: Fixed line scan rate.Timing diagram (level controlled mode from camera):BASLER L304kc August 15, 2006 Specifics about theCamera Interface Details (cont.)interface modesMode 6: Variable line scan rate with variable frame sizeLine/frame rate: The line rate is variable and controlled by the external linetrigger frequency. The default exposure time for this DCF is 130 μs. Thenumber of lines per virtual frame (maximum of 1000 for this DCF) is variableand controlled by the frame trigger signal. Matrox Solios eCL/XCL captureslines during the high level of the frame trigger signal. To modify the maximumamount of lines captured, change the active vertical timing period in the DCF.Capture of the lines will start with the rising edge of the frame trigger signal.Exposure time: Refer to Mode 1: Fixed line scan rate.Camera communication:Refer to Mode 2: Variable line scan rate.Timing diagram (level controlled mode from camera): Cabling details for theCabling Requirementsinterface modesMode 1: Fixed line scan rateCable and Connection: Standard Camera Link cable.Mode 2: Variable line scan rateCable and Connection: Standard Camera Link.External trigger: External line trigger should be connected to the OPTOTRIG input of the 9-pin connector (pins 7 and 2) on the External I/O adapterbracket:EXTERNAL I/O BRACKET(9-pin connector) External Trigger SourcePin Name Pin no. Pin NameOPTO_AUX_IN0 + 07 ←LINE TRIGGER (TTL FORMAT)OPTO_AUX_IN0 - 02 ←LINE TRIGGER (GROUND)BASLER L304kc August 15, 2006 Specifics about theCabling Requirementsinterface modesMode 3: Fixed line scan rate with frame triggerCable and Connection: Standard Camera Link.External trigger: External line trigger should be connected to the OPTOTRIG input of the 9-pin connector (pins 4 and 5) on the External I/O adapterbracket:EXTERNAL I/O BRACKET(9-pin connector) External Trigger SourcePin Name Pin no. Pin NameOPTO_AUX_IN1 + 04 ←FRAME TRIGGER (TTL FORMAT)OPTO_AUX_IN1 - 05 ←FRAME TRIGGER (GROUND)Mode 4: Variable line scan rate with frame triggerCable and Connection: Standard Camera Link.External trigger: External frame and line triggers should be connected to theOPTO TRIG inputs of the 9-pin connector on the External I/O adapterbracket:EXTERNAL I/O BRACKET(9-pin connector) External Trigger SourcesPin name Pin no. Pin nameOPTO_AUX_IN1 + 04 ←FRAME TRIGGER (TTL FORMAT)OPTO_AUX_IN1 - 05 ←FRAME TRIGGER (GROUND)OPTO_AUX_IN0 + 07 ←LINE TRIGGER (TTL FORMAT)OPTO_AUX_IN0 - 02 ←LINE TRIGGER (GROUND)Mode 5: Fixed line scan rate with variable frame sizeCable and Connection: Standard Camera Link.External trigger: External trigger should be connected to the OPTO TRIGinput (pins 4 and 5) of the 9-pin connector on the External I/O adapterbracket:EXTERNAL I/O BRACKET(9-pin connector) External Trigger SourcesPin name Pin no. Pin nameOPTO_AUX_IN1 + 04 ←FRAME TRIGGER (TTL FORMAT)OPTO_AUX_IN1 - 05 ←FRAME TRIGGER (GROUND)BASLER L304kc August 15, 2006 Cabling details for theCabling Requirementsinterface modesMode 6: Variable line scan rate with variable frame size▪Cable and Connection: Standard Camera Link.▪External trigger: External frame and line triggers should be connected to theOPTO TRIG inputs of the 9-pin connector on the External I/O adapterbracket:EXTERNAL I/O BRACKET(9-pin connector) External Trigger SourcesPin name Pin no. Pin nameOPTO_AUX_IN1 + 04 ←FRAME TRIGGER (TTL FORMAT)OPTO_AUX_IN1 - 05 ←FRAME TRIGGER (GROUND)OPTO_AUX_IN0 + 07 ←LINE TRIGGER (TTL FORMAT)OPTO_AUX_IN0 - 02 ←LINE TRIGGER (GROUND)The DCFs mentioned in this application note are also attached (embedded) to this PDF file – use the Adobe Reader’s View File Attachment to access the DCF files. The information furnished by Matrox Electronics System, Ltd. is believed to be accurate and reliable. Please verify all interface connections with camera documentation or manual. Contact your local sales representative or Matrox Sales office or Matrox Imaging Applications at 514-822-6061 for assistance. © Matrox Electronic Systems Ltd, 2006-2011.Matrox Electronic Systems Ltd.1055 St. Regis Blvd.Dorval, Quebec H9P 2T4CanadaTel: (514) 685-2630Fax: (514) 822-6273。
A new species of Ellobiophrya chatton et Lwoff,1923 Attached to Mantoscyphidia Jankowski
Acta Protozool. (2004) 43: 163 - 172 A New Species of Ellobiophrya Chatton et Lwoff, 1923 (Ciliophora: Peritrichia) Attached to Mantoscyphidia Jankowski, 1980 (Ciliophora: Peritrichia) SpeciesHelene PETERS, Liesl L. V AN AS, Linda BASSON and Jo G. V AN ASDepartment of Zoology and Entomology, University of the Free State, Bloemfontein, South AfricaSummary. Surveys carried out along the coast of South Africa revealed the presence of a secondary symbiont of the genus Ellobiophrya Chatton et Lwoff, 1923 found attached to the narrow basal part adoral to the scopula of Mantoscyphidia spadiceae Botes, Basson et Van As, 2001 and M. midae Botes, Basson et Van As, 2001 occurring on the gills of Haliotis spadicea Donovan, 1808 and H. midae Linnaeus, 1758, respectively. Mantoscyphidia branchi V an As, Basson et Van As, 1998 found on the gills of Cymbula H. et A. Adams, 1854 and Scutellastra H. et A. Adams, 1854 species respectively, had the same ellobiophryid species attached to the narrow part adoral to the scopula. This ellobiophryid differs from all the known Ellobiophrya species with respect to morphology of the body, features of the nuclear apparatus, and host preference and is therefore described as a new species, Ellobiophrya maliculiformis sp. n.Key words:Ellobiophrya maliculiformis sp. n., Mantoscyphidia, marine mollusc, scyphidiid peritrich, secondary symbiont.INTRODUCTIONRepresentatives of the family Ellobiophryidae Chatton et Lwoff, 1929 attach to the host by means of a scopula that has been adapted to form a ring-like cinctum or caudal process (Clamp 1982). Only parts of the scopula are included in the bouton of the cinctum, as the remainder of the scopula (the principal part) is found in the usual location and secretes the embryonic stalk linking the two daughters that result from binary fission.Currently the family comprises two genera, i.e. Ellobiophrya Chatton et Lwoff, 1923 and Caliperia Laird, 1953. All of the known species of the genus Ellobiophrya were found associated with fish, bivalves or bryozoan hosts from marine habitats. Ellobiophrya donacis Chatton et Lwoff, 1923 was described from the gill filaments of the bivalve Donax vittatus (Chatton and Lwoff 1923, 1928, 1929). Nearly sixty years later Clamp (1982) described E. conviva from the tentacles of the ectoprocts Bugula neritina and B. turrita. Another species, E. oblida (Naidenova et Zaika, 1969) occurs on the marine fish Proterorhinus marmoratus. It was originally described as Clausophrya oblida by Naidenova and Zaika (1969), but was placed within the genus Ellobiophrya by Clamp (1982).Address for correspondence: Liesl L. van As, Department of Zoology and Entomology, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa; Fax: (+2751) 448 8711; E-mail: V ANASLL.SCI@MAIL.UOVS.AC.ZA164 H. Peters et al.Caliperia longipes Laird, 1953 and C.brevipes Laird, 1959 were both described from the gill filaments of marine fishes (Laird 1953, 1959). This genus is characterised by a non-contractile skeletal rod within the arms of the cinctum and by not having the cinctal arms bonded to one another at the tips. Clamp and Bradbury’s (1997) observations, however, revealed that the cinctal arms of C.brevipes are linked by a bouton and that the cytoskeletal structure within them has the fine structure of a myoneme. These characteristics place C.brevipes in the genus Ellobiophrya. This species was renamed as E. brevipes (Laird, 1959) with C. longipes the sole remaining species in the genus (Clamp and Bradbury 1997). According to Clamp, the genus Caliperia may not exist at all, and if C. longipes could be recollected someday, it may also turn out to be an Ellobiophrya (Clamp, personal comm.)1.The ellobiophryid found in this study belongs to the genus Ellobiophrya,based on the morphology of the cinctum and the presence of a bouton. The same Ellobiophrya species was attached around the body of various scyphidiid peritrich hosts adoral to the scopula. The hosts were populations of Mantoscyphidia spadiceae Botes, Basson et Van As, 2001, M. midae Botes, Basson et Van As, 2001 and M. branchi Van As, Basson et Van As, 1998, which occur on the gills of Haliotis spadicea Donovan, 1808, H. midae Linnaeus, 1758 and different limpet species, respectively (Van As et al. 1998, Botes et al. 2001). This ellobiophryid differs from the known species with respect to morphological features of the body, characteristics of the nuclear apparatus, and host preference and is described as a new species.MATERIALS AND METHODSSouth African haliotids, i.e. Haliotis spadicea (Venus Ears) and H. midae (Perlemoen) were collected from infratidal pools on the rocky shores along the south coast of South Africa. The haliotids hosted two scyphidiid peritrich species, Mantoscyphidia spadiceae and M. midae. Mantoscyphidia branchi was found on the gills of all the limpet species collected from the rocky shore along the south, west and east coast of South Africa. Gills were dissected, placed on a microscope slide, smeared, and examined using a compound micro-scope. Live specimens of ellobiophryids were observed and photo-micrographs were taken of ellobiophryids found associated with Mantoscyphidia spadiceae and M. midae for the purpose of measur-ing body dimensions. The species is described from the type popula-tion, found attached to the host Mantoscyphidia spadicea. Additional data and measurements from the other host populations, namely M. midae and M. branchi, are given in Table 1.Additionally, wet smears were fixed in Bouin’s fluid, transferred to 70% ethanol and stained with Heidenhain’s Iron, Mayer’s and Harris’ Hematoxylin for studying the nuclear apparatus and for measuring body dimensions. In order to study details of the in-fundibulum, Bouin’s-fixed smears were stained with protargol, ini-tially using a combined method as described by Lee et al. (1985) and Lom and Dykova (1992). This method proved rather unsuccessful, as the ellobiophryids had many symbiotic algae and inclusions, which obscures the position of the infraciliature. Clamp’s “quick method”(Clamp, personal communication) which is an adaptation of the method of Wicklow and Hill (1992), gave the best results. A brief summary of the method is: Bouins-fixed smears were transferred to 70% ethanol, then 50%, 30% and distilled water: followed by bleach-ing in 0.5% potassium permanganate for 5 min and washed in distalled water; transferred to 5% oxalic acid for 5 min and washed for 10 min; some slides were placed in 1% protargol solution for 10-15 min at 67-70°C, with copper sheets and others for a period of 12-24 h at room temperature; transferred to 1% hydroquione (in 5% sodium sulfite) for 7-8 min, washed briefly; transferred to 0.5% gold chloride for 15 s, washed briefly; transferred to 2% oxalic acid for up to 3 min; remove and washed for 5 min; transferred to 5% sodium thiosulfate for 5 min and washed in distilled water for 5 min; slides were dehydrated in 30, 50, 70, 95, 100% ethanol; transferred to xylene and mounted using Canada Balsam.For scanning electron microscopy (SEM), gills were fixed in 4% and 10% buffered neutral formalin. In some cases, gills were fixed in Parducz and 2.5% glutaraldehyde. In the laboratory in Bloemfontein, the specimens were cleaned by washing gills in tapwater, dehydrated in a series of ethanol concentrations and critical-point dried. Gills bearing ellobiophryids attached to mantoscyphidians were mounted on stubs, sputter-coated with gold and studied at 5kV and 10kV, using a JOEL WINSEM JSM 6400 scanning electron microscope.For measurements of live specimens, minimum and maximum values are given, followed in parentheses by the arithmetic mean, standard deviation and number of specimens measured. Measure-ments based on Bouin’s-fixed specimens stained with hematoxylin are presented in square brackets. Body length is measured from the epistomial disc to the cinctum and body diameter at the widest part of the body. Description of pellicular striations was done from speci-mens viewed by SEM. The type material is in the collection of the National Museum, Bloemfontein, South Africa.RESULTS AND DISCUSSIONEllobiophrya maliculiformis sp. n. (Figs 1-13) Type host and locality:Mantoscyphidia spadiceae Botes, Basson et Van As, 2001, attaches to narrow basal part adoral to the scopula; De Hoop Nature reserve, south coast (34°28’S, 20°30’E) of South Africa.Other hosts and localities:M. midae Botes, Basson et Van As, 2001 and M. branchi Van As, Basson et V an1 Dr. John C. Clamp, Department of Biology, North Carolina Central University, Durham, North Carolina, 27704, USA.Ellobiophrya attached to peritrichs165 As, 1998, De Hoop Nature reserve, south coast (34°28’S,20°30’E) and Papendorp, Olifants River mouth, westcoast of South Africa (31°40’S, 18°15’E).Type specimens: Holotype, slide 98/04/11-04 (NMBP282), Paratype slides 98/04/04-05 (NMBP 283), 97/04/05-04c (NMBP 284), in the collection of the National Museum, Bloemfontein, South Africa.Etymology: Named after the mode of attachment adoral to scopula of the hosts, which resembles hand-cuffs.DescriptionTrophont conical, elongate, tapering aborally towards scopular region (Figs 1, 3, 4, 9-12). Length of body 50-125 µm (78.5 ± 15.1, 40) [60-98 µm (70.2 ± 17.5, 43)],Fig. 1. Microscope projection drawing of Ellobiophrya maliculiformis sp. n. found as a secondary symbiont on Mantoscyphidia spadiceae Botes, Basson et V an As, 2001; M. midae Botes, Basson et Van As, 2001 and M. branchi Van As, Basson et Van As, 1998 occurring on the gills of Haliotis spadicea Donovan, 1808; H. midae Linnaeus, 1758 and Scutellastra barbara (Linnaeus, 1758) collected along the southcoast of South Africa. ad - adoral ciliary spiral, c - cinctum, cj - cinctal junction, cl - cinctal limb, ma - macronucleus, mi - micronucleus, pl - peristomial lip, ps - pellicle striations, sa - symbiotic algae. Scale bar 10 µm.Fig. 2. Diagrams illustrating the infraciliature of Ellobiophrya maliculiformis sp. n. found as a secondary symbiont on Mantoscyphidia spadiceae Botes, Basson et Van As, 2001, M. midae Botes, Basson et Van As, 2001 and M. branchi Van As, Basson et Van As, 1998 occurring on the gills of Haliotis spadicea Donovan, 1808, H. midae Linnaeus, 1758 and Scutellastra barbara (Linnaeus, 1758) collected along the south coast of South Africa. A - haplo- and polykinetids. B - infundibulum. a - ampulla, c - cinctum, cp - cytopharynx, cs - cytostomal sphincter, hk - haplokinety, i - infundibulum; m - myoneme, pk - polykinety, s - scopula,tb - telotroch band, tp - third polykinetid. Scale bars 10 µm.166 H. Peters et al.Table 1. Body measurements (µm) of live observations (A) and hematoxylin-stained specimens (B-F) of Ellobiophrya maliculiformis sp. n. from Mantoscyphidia midae Botes, Basson et V an As, 2001 and M. branchi Van As, Basson et Van As, 1998 occurring on the gills of haliotid (A,B) and different limpet (C-F) species from the south coast of South Africa.Mollusc host A(Haliotis B (H. midae)C(Scutellastra D (S. argenvilli)E(S. cochlear)F(Cymbula midae)barbara)compressa) Ciliate host M. midae M. midae M. branchi M. branchi M. branchi M. branchiBody length60.0-85.043.0-93.045.0-65.045-8340-7051-70(72.9 ± 8.4, 20)(61.9 ± 13, 35)(56.5 ± 6.4, 9)(62.6 ± 11.9, 18)(56.5 ± 9.8, 12)(60.0, 5) Body diameter15.0-25.013.0-29.020-3115-3013-2618-39(20.1 ± 2.4, 20)(23.1 ± 3.9, 35)(26.3 ± 3.6, 9)(21.3 ± 3.7, 18)(18.6 ± 4.1, 12)(29.4, 5) Outer cinctum12.0-15.0-119-1713-16-diameter(13.5 ± 2.1, 2)(12.6, 5)(14.5, 4)Inner cinctum--12-105-10-diameter(6, 5)(7.2, 4)Limb diameter- 1.0-6.02-42-62-52-3(2.2 ± 1, 30)(2.3, 8)(3.1 ± 1.1, 17)(3.4 ± 0.9, 11)(2.8, 5) Table 2. Body striations of Ellobiophrya maliculiformis sp. n. found attached to Mantoscyphidia spadiceae Botes, Basson et Van As, 2001 and M. branchi V an As, Basson et Van As, 1997 occurring on the gill filaments of Haliotis spadicea Donovan, 1808 and Scutellastra barbara (Linnaeus, 1758)* respectively from the south coast of South Africa.Number of striations Number of striationsHost M. spadiceae Host M. branchiPeristome8-227-9(14.2 ± 4.6, 10)(8.0, 3)Peristome to cinctum54-11866-96(86.7 ± 17.3, 10)(78.6 ± 8.8, 13) Total number of striations62-14066-116(100.9 ± 20.5, 10)(82.4 ± 12.8, 13)* A new phylogenetic classification for the patellid limpets was suggested by Ridgway et al. (1998), grouping the patellid limpets in four monophyletic genera, namely Helcion Montfort, 1810; Cymbula H. et A. Adams, 1854; Scutellastra H. et A. Adams, 1854 and Patella Linnaeus, 1758, with the latter genus not occurring in South Africa. All limpets were formerly placed in the genus Patella.Figs 3-13. Scanning electron micrographs (4, 6-8) and photomicrographs of live (3, 13) and protargol stained specimens (5, 9-12) of Ellobiophrya maliculiformis sp. n. occurring as a secondary symbiont on Mantoscyphidia spadiceae Botes, Basson et Van As, 2001, M. midae Botes, Basson et Van As, 2001 and M. branchi V an As, Basson et Van As, 1998 on the gills of Haliotis spadicea Donovan, 1808, H. midae Linnaeus, 1758 and Scutellastra barbara (Linnaeus, 1758) collected along the south coast of South Africa. 3 - live specimen of E. maliculiformis with protruding adoral cilia attached to M. spadiceae; 4 - detached E. maliculiformis, upper part of the body partially contracted; 5 - bifurcated structure at the tip of the myoneme in the cinctum, scopula indicated by arrow; 6 - cinctum, scopula indicated by arrow; 7 - one limb of cinctum tapers, fitting into the cinctal junction of the shorter, broader limb; 8 - attachment of cinctum around Mantoscyphidia spadiceae; 9-12 - protargol-stained specimens; 13 - microconjugant attached to ellobiophryid associated with M. spadiceae. a - ampulla, c - cinctum, cl - cinctal limb, cp - cytopharynx, cs - cytostomial sphincter, m - myoneme, mc - microconjugant, pl - peristomial lip, s - scopula,sa - symbiotic algae, tb - telotroch band. Scale bars 10 µm (3, 4, 9-13), 1 µm (5-8).Ellobiophrya attached to peritrichs167168 H. Peters et al.diameter of body 15-30 µm (20.5 ± 3.7, 40)[16-37 µm (23.9 ± 5, 43)]. Peristome with broad, striated peristomial lip (Fig. 3); zig-zag striations present on peristome in contracted specimens. Prominent peristo-mial sphincter (Figs 10, 11). Striations on peristome not always visible. Body striated; 101 striations on average,spaced 0.5 µm apart. Striations evenly spaced and uniform (Fig. 4, Table 2).Trochal band narrow, slightly elevated, one quarter length of body from cinctum, not always clearly visible (Figs 4, 11). Cinctum flattened with two cytoplasmic cinctal limbs of uneven thickness forming closed circle (Figs 4-8). One limb tapers, fitting into cinctal junction of shorter somewhat broader limb, both limbs terminate at bouton (Fig. 7). Limb that tapers forms bifurcated structure at tip of its myoneme (Fig. 5). Scopula is typical, but nonfunctional, except when it participates in secretion of larval stalk that links two daughters after fission (Figs 2B, 4-6).Oral infraciliature of E. maliculiformis divisible into peristomial part and infundibular part as in other sessiline peritrichs. Adoral zone completes spiral of 360° counter-clockwise around epistomial disc, with haplo- and polykinety starting almost at same point. Peristomial part consists of outer band of kinetosomes (polykinety) and inner band of kinetosomes (haplokinety) which parallel one another for entire length before plunging into in-fundibulum (Fig. 2A).Haplokinety and polykinety run together around peris-tome and separate before plunging into infundibulum.Polykinety joined by additional polykinetids after entering infundibulum. P1 and P2 were positively observed inmost of the specimens. The third polykinetid, which is normally very short, was observed only in few speci-mens, running parallel and closely associated with polykinety from the lip of opening up to first turn within infundibulum. Inside infundibulum, polykinetids and haplokinety make one turn (360°- 400°) each on opposite walls, before reaching cytostome.Conspicuous cytostomial sphincter visible at end of infundibulum which constricts area between infundibu-lum and cytostome (=ampulla) (Figs 9-11). Ampulla tubular when empty and slighty bulbous when filled with food (Figs 9-12). Ampulla merges with cytopharynx that is very small in diameter throughout its length, recurving slightly just adoral to trochal band (Figs 2B, 11, 12).Symbiotic algae present throughout cytoplasm, vary-ing in number and size, obscuring position and shape of nuclear apparatus (Fig. 3). Micronucleus fusiform, but not always visible. Macronucleus coiled and sausage-shaped, extending throughout body. Prominent sections of nucleus visible in adoral and aboral sides (Fig. 1).Reproduction is by means of conjugation and binary fission followed by telotroch formation. Ellobiophryids in various stages of binary fission were observed as well as individuals with attached microconjugants (Fig. 13), which confirms the first record of conjugation (Fig. 13) in the genus Ellobiophrya (Botes et al . 2001). Live observa-tions of conjugation were made in two instances in populations on M. midae , four times in populations on M. spadiceae (Fig. 13), and twice in populations on M. branchi .Binary fission and the subsequent formation of telotrochs were observed in ellobiophryid populations associated with all three hosts. After binary fission one daughter individual becomes a telotroch and the other remains a trophont attached by the parental cinctum to the host. As in other ellobiophryids, the telotroch is attached during development to the trophont daughter by a short, rigid stalk that passes between the scopulas of the two individuals (Bradbury and Clamp 1991). The telotroch is slightly asymmetric, as is the case in other Ellobiophrya species.A larval stalk was identified during an observation of telotroch formation in a live specimen of Ellobiophrya maliculiformis attached to M. midae . The telotroch was attached to the trophont daughter by this short stalk,and the trochal band of cilia was in the process of differentiating, but was not beating yet. The parent’s peristome was open, with cilia creating a feeding cur-rent. This telotroch was found on a gastropod host that had been collected 8-10 h beforehand and was observedFig. 14. Comparison in the variation of body length, body diameter and cinctal diameter of different populations of Ellobiophrya maliculiformis sp. n. found attached to Mantoscyphidia midae Botes,Basson et Van As, 2001 (B) and M. branchi Van As, Basson et V an As,1998 (C-F) associated with haliotid (B) and limpet species (C-F)collected along the south coast of South Africa.Ellobiophrya attached to peritrichs169for a period of 55 min before it separated from the parent and swam away. The aboral end (scopula) that was attached to the embryophore of the parent ellobiophryid became broader after separation.A telotroch-like individual was also observed attached to the body of a trophont of E. maliculiformis. It was attached to the middle region of the trophont, and it may have been a microconjugant that had just attached in preparation for conjugation, rather than a telotroch that was preparing to separate from the other daughter. This telotroch had a short, stalk-shaped structure which at-tached it to the trophont, but it was not attached to the scopula, as would have been the case in a developing telotroch. The apparent stalk may have been a slender cytoplasmic connection because a larval stalk is ex-pected to be linked to the scopula (embryophore) of the trophont daughter.Intraspecific variationBody measurements of live observations and he-matoxylin-stained material of E. maliculiformis are summarised in Table 1. The effect of contraction on the body length of live specimens versus hematoxylin-stained specimens is as follows: in the M. spadiceae populations there was a 27% body contraction between live obser-vations and those stained with hematoxylin. The length and diameter of the body varied among different popu-lations of E. maliculiformis. The average body length of ellobiophryid populations on M. midae was 61.9 µm. The average body length of populations found associated with M. branchi, ranged between 56.5 and 62.6 µm (Table 1).Ellobiophryids from M. branchi, M. spadiceae and M. midae had the same body form. The ratio of body length to diameter in hematoxylin-stained specimens of E. maliculiformis found on M. midae and M. branchi is as follows: 2.68 (M. midae), 2.15 (M. branchi), 2.94 (M. branchi), 3.04 (M. branchi) and 2.04 (M. branchi). Also no significant differences could be found in the diameter of the cinctal limbs of different populations (see Table 1 and Fig. 14).Live specimens of E. maliculiformis from M. spadiceae were extremely contractile, with body length ranging between 50 and 125 µm. The body of M. branchi is also extremely contractile, with fully expanded specimens varying from 40 µm to 95 µm. Van As et al. (1998) observed during fieldwork that the same individual of M. branchi could achieve a reduction in body length with the peristome remaining open. In these specimens, groups of elevated striations can be seen aboral to the telotroch band. When the peristome of M. branchi is fully closed, the degree of contraction can also vary. Specimens of M. branchi can be found in a whole range of body contractions on a single smear. Live ellobiophryids were able to contract to half of their fully extended body length.Although the nuclear apparatus of all the populations were mostly obscured by algal inclusions, there were no great differences in the shape of the macronucleus. It is coiled and stretches throughout the body, much the same as those of E. conviva, E. oblida and E. brevipes (Clamp and Bradbury 1997).The only difference between the ellobiophryid popu-lations of M. spadiceae and M. branchi was that the latter had slightly fewer body striations (Table 2). This could be due to the fact that E. maliculiformis speci-mens found associated with M. spadiceae has a greater body length. The M. spadiceae population had a preva-lence of 35.4% of ellobiophryids associated with the scyphidiid peritrichs, and the M. midae population had a prevalence of 34.3%, whilst those ellobiophryids found associated with M. branchi had a prevalence of 17%. RemarksThis is the first record of an ellobiophryid from Africa and the first found associated with another ciliophoran host in the marine environment. Other records of peritrichs found in a symbiotic association with peritrichs, are that of Epistylis lwoffi Fauré-Fremiet, 1943 which attached to the epistylidid Apiosoma piscicola (Blanchard), which in turn is found on the skin of freshwater fish (Fauré-Fremiet 1943, Clamp 1982) and E. colisarum (Foissner et Schubert, 1977) attaching to an epistylidid which lives symphoriontly on a freshwater fish, Colisa fasciata (Anabantoidei: Belontiidae) (Foissner and Schubert 1977).In comparing E. maliculiformis with other species of Ellobiophrya, it shows the most resemblance to E. oblida in respect to body form. In both E. oblida and E. maliculiformis, the expanded peristome is wider in diameter than the rest of the body, and the peristomial lip is everted. Ellobiophrya oblida is, however, a much larger species than E.maliculiformis and has a different host and site preference as it occurs on the skin of marine fish. The position of the scopula of E. maliculiformis differs from the other species of Ellobiophrya in that it is located much nearer to the cinctum. The cinctum of E.maliculiformis is also asymmetrical, with uneven limbs. The longer thinner limb fits into the junction of the shorter much broader limb. The limb diameter of the populations varies be-170 H. Peters et al.T a b l e 3. S u m m a r y o f t h e t a x o n o m i c c h a r a c t e r i s t i c s o f t h e s p e c i e s o f t h e f a m i l y E l l o b i o p h r y i d a e : E l l o b i o p h r y a d o n a c i s C h a t t o n e t L w o f f , 1923, E . c o n v i v a C l a m p , 1982, E . o b l i d a (N a i d e n o v a e t Z a i k a , 1969), E . b r e v i p e s (L a i r d , 1959), E . m a l i c u l i f o r m i s s p . n . a n d C a l i p e r i a l o n g i p e s L a i r d , 1953. M a - m a c r o n u c l e u s , M i - m i c r o n u c l e u s .S p e c i e s E . d o n a c i sE . c o n v i v aE . o b l i d a E . b r e v i p e s E . m a l i c u l i f o r m i s C . l o n g i p e sH o s tD o n a x v i t t a t u s B u g u l a n e r i t i n a ,P r o t e r o r h i n u s R a j a e r i n a c e a M a n t o s c y p h i d i a s p a d i c e a e ,O l i v e r i c h t u s m e l o b e s i ,M a r i n e b i v a l v e B . t u r r i t a M a r i n e m a r m o r a t u s M a r i n e f i s h M . m i d a e , M b r a n c h i ,E r i c e n t r u s r u b r u s ,m o l l u s ce c t o p r o c t s (B r y o z o a )M a r i n e f i s h S c y p h i d i i d p e r i t r i c h s M a r i n e f i s h e sP o s i t i o n o n h o s t G i l l f i l a m e n t sC i l i a t e d t e n t a c l e s S k i n G i l l s N a r r o w b a s a l G i l l sa r o u n d m o u t hp a r t a d o r a l t o s c o p u l a o f h o s t C o l l e c t i o n l o c a l i t y M o r g a t , F r a n c eN o r t h C a r o l i n a , U S AB l a c k S e a N e w B r u n s w i c k ,S o u t h c o a s t o f W e l l i n g t o n ,C a n a d a S o u t h A f r i c aN e w Z e a l a n dB o d y l e n g t h (µm )50 (100)46.218060.2 (54.5)50-125 (78.5)31.2-68.4 (51.5)B o d y d i a m e t e r (µm )40 (30)26.836.534.6 (35.7)15-30 (20.5)24.0-52.6 (38.8)B o d y a n d n u c l e iB o d y s u b c y l i n d r i c a l ,B o d y s u b c y l i n d r i c a l ,B o d y c y l i n d r i c a l ,B o d y c y l i n d r i c a l ,B o d y c o n i c a l ,B o d y c y l i n d r i c a l ,e l o n g a t e , t a p e r s e l o n g a t e s l i g h t l y ,s u b c o n i c a l ,e l o n g a t e , s u b c o n i c a l ,e l o n g a t e , t a p e r s t a p e r s t o w a r d s t o w a r d s o r a l p o l e t a p e r s t o w a r d s t a p e r s t o w a r d s t a p e r s t o w a r d s t o w a r d s a b o r a l p o l e ,a b o r a l p o l e ,M a - c o m p a c t a b o r a l p o l e a b o r a l p o l e a b o r a l p o l e ,M a - c o i l e d ,M a – c y l i n d r i c a l ,a n d s p h e r i c a l ,M a - c y l i n d r i c a l ,M a - c y l i n d r i c a l ,M a - c y l i n d r i c a l ,s a u s a g e - s h a p e d ,M i - f u s i f o r m /l e n t i c u l a rM i - f u s i f o r ml e n g t h o f s o m a ,M i - f u s i f o r m l o n g a n d f l a t ,M i - f u s i f o r mM i - f u s i f o r m /o v a lM i - f u s i f o r m C i n c t u mL i m b s j o i n e d ,L i m b s j o i n e d L i m b s j o i n e d ,L i m b s j o i n e d ,L i m b s o f u n e v e n L i m b s n o t j o i n e d ,b o u t o n , n o (c e m e n t e d a t t i p s ),b o u t o n , n o b o u t o n , n o t h i c k n e s s ,n o b o u t o n , 5-6 µmi n t e r n a l r o d b o u t o n , n o i n t e r n a l r o d ,i n t e r n a l r o d ,f i t t i n g i n t o m y o n e m e ,i n t e r n a l r o d ,m y o n e m e m y o n e m ej u n c t i o n , b o u t o n ,c o n t r a c t i l ec o n t r a c t i l en o i n t e r n a l r o d ,m y o n e m eA m p u l l a s h a p eN a r r o w a n d W i d e a n d N o t d e s c r i b e dL o n g , s l e n d e r ,T u b u l a r w h e n R e s e m b l e s p i p e t t e l a n c e o l a t eb u l b o u s t a p e r i n g s m o o t h l y e m p t y , b u l b o u s b u l b ,c y t o s t o m a l i n t o c y t o p h a r y n x ,w h e n f i l l ed ,s p h i n c te r b e t w e e n s m a l l i n d i a m e t e r ,m e r g e s w i t h i nf u n d i b u l u m e l o ng a t e , a l m o s t c y t o ph a r y n xa n d a m p u l l at u b u l a r , n a r r o w ,l a n c e o l a t e w h e n n o t f i l l e d。
00Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties
Production of a biopolymer¯occulant from Bacillus licheniformis andits¯occulation propertiesI.L.Shih a,*,Y.T.Van a,L.C.Yeh a,H.G.Lin a,Y.N.Chang ba Department of Environmental Engineering,Da-Yeh University,112Shan-Jiau Road,Da-Tsuen,Chang-Hwa51505,Taiwan,ROCb Department of Food Engineering,Da-Yeh University,Chang-Hwa51505,Taiwan,ROCReceived14October2000;received in revised form16December2000;accepted22December2000AbstractBacillus licheniformis CCRC12826produced extracellularly an excellent biopolymer¯occulant in a large amount when it was grown aerobically in a culture medium containing citric acid,glutamic acid and glycerol as carbon sources.The biopolymer ¯occulant was an extremely viscous material with a molecular weight over2Â106by gel permeation chromatography.It could be easily puri®ed from the culture medium by ethanol precipitation.It was shown to be a homopolymer of glutamic acid by amino acid analysis and thin layer chromatography and presumed to be poly-glutamic acid(PGA).This bio¯occulant e ciently¯occulated various organic and inorganic suspensions.It¯occulated a suspended kaolin suspension without cations,although its¯occulating activity was synergistically stimulated by the addition of bivalent or trivalent cations Ca2 ,Fe3 and Al3 .However,the synergistic e ects of metal cations were most e ective at neutral pH ranges.The comparison of the¯occulating activity between the present biopolymer and a commercial lower molecular weight product showed that the biopolymer of the present study had much higher activity.The high productivity and versatile applications of PGA make its development as a new biodegradable,harmless, biopolymer¯occulant economical and advantageous.Ó2001Elsevier Science Ltd.All rights reserved.Keywords:Bio¯occulant;Polyglutamic acid;Bacillus licheniformis1.IntroductionFlocculating agents are generally categorized into three major groups,i.e.,inorganic¯occulants:alumini-um sulfate and polyaluminium chloride(PAC);organic synthetic polymer¯occulants:polyacrylamide deriva-tives,polyacrylic acids,and polyethylene imine;natu-rally occurring bio-polymer¯occulants:chitosan,algin, and microbial¯occulants.These¯occulants have been widely used in wastewater treatment and in a wide range of industrial downstream processes(Gutcho,1977;Na-kamura et al.,1976;Kurane et al.,1986).Among these ¯occulants,the organic synthetic polymer¯occulants are most frequently used because they are economical and highly e ective.However,their use often gives rise to environmental and health problems in that some of them are not readily biodegradable and some of their degraded monomers,such as acrylamide,are neuro-toxic and even strong human carcinogens(Vanhorick and Moens,1983;Dear®eld and Abermathy,1988). Thus,the development of a safe biodegradable¯occu-lant that will minimize environmental and health risks is urgently required.In recent years,the use of bio-poly-mers produced by microorganisms has been investigated. These microbial polymers are expected to be useful ¯occulating agents due to their bio-degradability and the harmlessness of their degradation intermediates toward humans and the environment.Several bio¯occ-ulants from di erent microorganisms have been reported recently,they were characterized as proteins,poly-saccharides,and glycoprotein.It has been shown that Rhodococcus erythropolis S-1(Kurane et al.,1986; Takeda et al.,1991),Nocardia amarae YK1(Takeda et al.,1992),and Pacilomyces sp.(Takagi and Kado-waki,1985)produced protein¯occulants.Alcaligenes latus B-18(Kurane and Nohata,1991),Alcaligenes cu-pidus KT201(Toeda and Kurane,1991),and Bacillus sp. DP-152(Suh et al.,1997)produced polysaccharide ¯occulants.The¯occulants produced by Arcuadendron sp.TS-4(Lee et al.,1995),and Arathrobacter sp.(Wang et al.,1995)were glycoproteins.In this study,attention was focused on the microbial¯occulant of BacillusBioresource Technology78(2001)267±272 *Corresponding author.Tel.:+886-4-852-3870;fax:+886-4-852-3870.E-mail address:ils@.tw(I.L.Shih).0960-8524/01/$-see front matterÓ2001Elsevier Science Ltd.All rights reserved. PII:S0960-8524(01)00027-Xlicheniformis.Recently,the use of B.licheniformis CCRC12826for protease production has been investi-gated.It was found that the culture broth obtained was highly viscous when B.licheniformis CCRC12826was cultivated in a medium containing citric acid,glutamic acid and glycerol.Furthermore,the viscous culture broth displayed high¯occulating properties.Therefore, attention turned to characterizing this viscous biopoly-mer,and investigating the factors that a ect its¯occu-lating properties.The results of this investigation are reported herein.2.Methods2.1.MaterialsReagents for cultivation such as nutrient agar(NA), nutrient broth(NB)were purchased from DIFCO Laboratories Michigan,ponents of Medium, glutamic acid,citric acid,glycerol,NH4Cl,MgSO4 7H2O,FeCl36H2O,K2HPO4,and CaCl27H2O were obtained from Sigma Chemicals,USA.Kaolin(Practi-cal Grade)was purchased from Wako Pure Chem.Ind. Ltd.,Osaka,Japan.Activated carbon,cellulose,acid clay and aluminum oxide were from Sigma Chemicals, USA.All other reagents used were of the highest grade available.2.2.Strain and culture conditionsB.licheniformis CCRC12826was obtained from the Culture Collection and Research Center(CCRC)Tai-wan,as a lyophilized powder in a glass ampoule sealed under vacuum.The bacterium was®rst cultured on Nutrient Broth plates(Difco Laboratories)containing agar(15g/l),beef extract(3g/l),peptone(5g/l)to induce spore formation.After cultivation(37°C,pH7.4)over-night,highly mucoid colonies that appeared on the plates were inoculated into5ml of Nutrient Broth (Difco Laboratories)composed of beef extract(3g/l), peptone(1.5g/l),and NaCl(5g/l),pH7.4in a30ml test tube,and then incubated at37°C for48h with shaking at150rpm.After incubation,the culture was inoculated into100ml of Medium(5%v/v)composed of glutamic acid(20g/l),citric acid(12g/l),glycerol(120g/l),NH4Cl (7.0g/l),MgSO47H2O(0.5g/l),FeCl36H2O(0.04g/l), K2HPO4(0.5g/l),and CaCl27H2O(0.15g/l)in a500-ml ¯ask(Leonard et al.,1958).The culture was incubated at37°C,pH6.5with shaking at150rpm.The produc-tion of the bio¯occulant was monitored by measuring the viscosity of the culture broth.At the end of the cultivation,a viscous culture broth was obtained,which was centrifuged(20,000Âg,15min)to separate the cells and tested for¯occulating activities.The viscous mate-rials were further puri®ed by the procedures described below.2.3.Flocculating activity of culture mediumThe¯occulating activities were measured using a previous method with a slight modi®cation,in which kaolin clay was chosen as the suspended solid(Kurane et al.,1986;Yokoi et al.,1995).Kaolin was suspended in distilled water or a bu er solution at a concentration of 5g/l.The kaolin suspension(9.3ml)was added to a test tube,and supplemented with various amounts of culture broth.The mixture was gently mixed and kept standing for5min at room temperature.The formation of visible ¯ocs was observed.The optical density(OD)of the upper phase was measured with a spectrophotometer (UV-260,Hitachi,Japan)at550nm.A control experi-ment without the culture broth was also measured in the same manner.Flocculating activity was calculated according to the following equation(Kurane et al., 1986,1994):Flocculating activity 1=OD550À1= OD550 c;OD550 Optical density of sample at550nm;OD550 c Optical density of control at550nm:2.4.Bio¯occulant puri®cationThe viscous culture broth was diluted with the same volume of distilled water and then centrifuged at 20,000Âg for15min.The supernatant was poured into four volumes of cold ethanol to precipitate the bio-polymer¯occulant.The resultant precipitate was col-lected by centrifugation at25,000Âg for15min and re-dissolved in distilled water.After three such ethanol precipitation steps,the crude biopolymer thus obtained was dialyzed at4°C overnight in de-ionized water and lyophilized to give pure material.2.5.Characterization of¯occulant2.5.1.Molecular weight determinationThe number-average molecular weight(M n)of the ¯occulant was measured by gel permeation chromato-graphy(GPC)using a Hitachi L6200system controller equipped with Shodex KB800series columns(two KB80M,one KB802.5),a refractive index(RI)detector (Bischo ,Model8110),a Waters Model730data module.Dextran-polysaccharide standards obtained from Phenomnex Inc.,USA were used to construct a calibration curve from which molecular weights of ¯occulants were calculated with no further correction. The eluant contained0.3M Na2SO4,0.05%(w/v)NaN3268I.L.Shih et al./Bioresource Technology78(2001)267±272was brought to a pH of4.0using glacial acetic acid,and the¯ow rate was set at1.0ml minÀ1.2.5.2.Amino acid analysis and thin layer chromatography The puri®ed material was hydrolyzed with6N HCl at 110°C for24h in a sealed and evacuated tube,and the amino acid compositions were determined with a Beckman system6300E analyzer.Thin-layer chromato-graphy was performed on a cellulose plate(Merck)with solvent systems of butanol±acetic acid±water(3:1:1,w/ w)and96%ethanol±water(63:37,w/w).Amino acids were detected by spraying with0.2%ninhydrin in ace-tone(Stewart and Young,1984;Yokoi et al.,1995).2.5.3.Viscosity measurementApparent viscosity of cell-free culture¯uid was measured with a Brook®eld Digital Rheometer(model DV-III,USA)equipped with a spindle SC4-34,at dif-ferent shear rates(0.28±56.0sÀ1).2.5.4.Protein and sugar contentsThe total carbohydrate content of the¯occulant was determined by the phenol±sulfuric acid method(Chaplin and Kennedy,1986)and expressed as the glucose equivalent.The protein moiety in the¯occulant mole-cule was determined by the Bradford method(Bradford, 1976)with bovine serum albumin as a standard.Sugar derivatives were investigated by the cabazoic method (Chaplin and Kennedy,1986)for uronic acid,the Friedmann method(Frideman and Haugen,1943)for pyruvic acid,and the hydroxamic acid method (McComb and McCreasy,1957)for acetic acid.2.6.Flocculating activity of pure¯occulant2.6.1.The e ects of¯occulant concentration and molec-ular weight on the¯occulating activityThe puri®ed¯occulant or a lower molecular weight PGA(poly-glutamic acid,Mwt.$1Â105)purchased from Sigma Chemicals was dissolved in distilled water to a ord a¯occulant solution of80mg/l.The kaolin sus-pension(5g/l)9.3ml was added to a test tube,and supplemented with various amounts of¯occulant solu-tion and90mM CaCl2solution(1.0ml).The¯occu-lating activities were then measured and calculated using the procedure described above.2.6.2.Flocculation of various suspended solidsThe¯occulating activities for various organic and inorganic solid suspensions,including activated carbon, cellulose,acid clay and aluminum oxide were also studied.Flocculation tests were carried out as described above with kaolin solution(5g/l)being replaced by the various solid suspensions(5g/l)as indicated.The solid suspension(9.3ml)was treated with0.5ml of¯occulant solution(80mg/l)and90mM CaCl2solution(1.0ml)and the¯occulating activities were measured.A soil suspension was prepared by adding500g of soil to1l of distilled water.After stirring and standing for3min,the upper phase was used for the¯occulation test.A cell suspension of Saccharomyces cerevisiae in distilled water was used as a yeast suspension.2.6.3.E ect of metal salts and pH on the¯occulating activity of¯occulantTo estimate the in¯uence of the salts,distilled water with5g/l of suspended kaolin was used as a test. Flocculation tests were carried out using the method mentioned above,except that the CaCl2solution was replaced by various metal salt solutions,with the¯oc-culation activity being similarly measured.Solutions of KCl,CaCl2,MgCl2,FeSO4Á7H2O,FeCl3Á6H2O and AlCl3Á6H2O were used as the sources of cations.To examine the e ects of pH values of the reaction mixture on¯occulating activity,the reaction mixtures composed of a kaolin suspension,¯occulant and various cations (Ca2 ,Mg2 ,Fe2 ,Fe3 or Al3 )were adjusted to pre-determined pH values using HCl and NaOH,¯occu-lating activities were then measured in the same manner as described above.The pH values of the test suspen-sions ranged from3.0to11.0.The OD of a test sus-pension free from salt was designated as OD550 c and the activity was calculated.The¯occulant productivites and the¯occulating ac-tivities of the¯occulants in this study were the mean value of two experiments.3.Results and discussion3.1.Production of bio¯occulantThe¯occulant productivity of B.licheniformis CCRC 12826was investigated using various nitrogen and car-bon sources.The e ects of carbon sources on bio¯occ-ulant production are shown in ctose,glucose, fructose were not favorable for growth and¯occulantTable1E ects of various carbon sources on¯occulant production of B.licheniformis CCRC12826Carbon source Isolated yield of¯occulant(g/l) Glucose ND aFructose NDLactose NDGlycerol0.1Citric acid0.6Glutamic acid0.4Citric acid Glutamic acid 1.6Citric acid Glycerol 1.0Glutamic acid Glycerol 4.3Glutamic acid Citric acid Glycerol14.2a Non-detectable.I.L.Shih et al./Bioresource Technology78(2001)267±272269production.In contrast,glutamic acid,citric acid,and glycerol were more favorable for growth and ¯occulant production.The e ects of these three carbon sources on ¯occulant production were synergistic.The e ects of nitrogen sources on ¯occulant production were also in-vestigated by cultivating the bacteria in the same medi-um,except that the nitrogen source was varied.As shown in Table 2,B.licheniformis CCRC 12826was able to use ammonium chloride e ectively,but unable to use other ammonium salts.When B.licheniformis CCRC 12826was cultured in medium containing glutamic acid (20g/l),citric acid (12g/l),glycerol (120g/l)as described in Section 2,it was observed that the medium became viscous.The course of ¯occulant production as well as changes of viscosity,pH of the medium,and the cell growth were monitored and shown in Fig.1.The pro-duction of ¯occulant,and the relative viscosity of the medium reached a maximum after 96h of incubation.The cell growth also reached a maximum after 96h.The overall product isolated after incubation for 96h was 14g/l.The pH pro®le showed that the pH fell from 6.5to 5.63in about 60h but rose afterward.3.2.Characterization of ¯occulantThe number-average molecular weight (M n )of the ¯occulant was determined by gel permeation chroma-tography and found to be over 2Â106.The 6N HCL hydrolysate of the puri®ed viscous material was com-posed solely of glutamic acid.Thin-layer chromato-graphy of the hydrolysate performed on a cellulose thin-layer plate and visualized with 0.2%ninhydrin in-dicated a single spot with a R f value identical to that of authentic glutamic acid.Furthermore,the ninhydrin and biuret reactions for the viscous material were neg-ative.The phenol±sulfuric acid method showed that the polysaccharide contained less than 1%(w/w)of total sugar.In the hydrolysate of the ¯occulant,less than 1%of acetic acid,pyruvic acid,and uronic acid were de-tected.From these results,the biopolymer was con-cluded to be poly (glutamic acid).3.3.Flocculating activity of culture broth and puri®ed ¯occulant3.3.1.The e ects of ¯occulant concentration and molec-ular weight on the ¯occulating activityThe ¯occulating activity of the culture broth was es-timated by the addition of various amounts of culture broth to a constant concentration of kaolin suspension (5000mg/l).The ¯occulating activity reached a maxi-mum when 1.5ml of culture broth was added.In a same manner,the e ect of the concentration of puri®ed ¯occulant on the ¯occulating activity was investigated by addition of various amounts of ¯occulant solution (80mg/l)to a constant concentration of kaolin suspen-sion (5000mg/l)containing 9.0mM CaCl 2.The ¯occu-lating activity increased in proportion to the amount of added ¯occulant up to 0.5ml of ¯occulant solution which gave the highest ¯occulation.The optimal ¯occ-ulant concentration in test solution was 3.7mg/l.The ¯occulating activity was clearly reduced when lower molecular weight PGA was tested,but the optimal concentration was the same as that of the experimental PGA.The result of the comparative study between the puri®ed ¯occulant and the commercial PGA ¯occulant indicated the fact that the molecular weight of a ¯occ-ulant as related to the chain length of the polymer is an important factor in the ¯occulating reaction.A large-molecular-weight ¯occulant usually is long enough and has a su cient number of free functional groups which can act as bridges to bring many suspended particles together,and hence cause a larger ¯oc size in the ¯oc-culating reaction (Gutcho,1977;Michaels,1954).It is known that the molecular weights of PGA varied from 100,000to 2,000,000depending upon the species and the cultivation time used to produce them.Whether the variation of molecular weight of PGA of di erentTable 2E ects of various nitrogen sources on ¯occulant production of B.licheniformis CCRC 12826Nitrogen sources Isolated yield of ¯occulant (g/l)NH 4Cl 13.9NH 4NO 3ND a (NH 4)2SO 40.2Peptone ND Urea0.3aNon-detectable.Fig.1.Course of ¯occulant (PGA)production,changes of viscosity,pH and bacterial growth.In the upper panel:(r )pH;(j )OD.In the lower panel:(r )viscosity;(j )PGA.The PGA productivities were according to the isolated yields deriving from the procedures described in Section 2.270I.L.Shih et al./Bioresource Technology 78(2001)267±272species has any in¯uence on the¯occulating activity needs further clari®ed.3.4.E ects of various salts and pH on the¯occulating activityFlocculating activity was markedly increased by the addition of Ca2 and Fe3 ,but decreased by the addition of the trivalent cation Al3 compared with that of the control where distilled water was used instead of cation solutions.The concentration of Ca2 for maximum ¯occulating activity was13.5mM.The dramatic decrease of¯occulating activity by addition of trivalent Al3 was probably due to the drop of pH to about3.5when this cation(as AlCl36H2O)was added into the solutions. To examine the e ects of pH of reaction mixture on ¯occulating activity,the pH of a reaction mixture con-taining kaolin suspension,9.0mM of cations(Ca2 ,Fe3 or Al3 )and biopolymer¯occulant(0.5ml,80mg/l) was adjusted with HCl and NaOH,and then¯occulat-ing activity was measured.The optimal¯occulating ac-tivities for Ca2 ,Fe3 and Al3 were near the neutral pH range;7.0,6.4and7.1for Ca2 ,Fe3 and Al3 ,re-spectively.Kaolin dissolved easily at a pH below3.0,a phenomenon that makes the accurate measurement of ¯occulating activity di cult under this pH.Highly synergistic e ects with addition of bivalent or trivalent cations were observed.Although the synergistic e ects were exerted at di erent pH depending on the cations,the synergistic e ects of the trivalent cations were stronger than the bivalent cations,with Al3 being the most e ective cation at neutral pH.These results suggest that¯occulation is due to change in the charge density,they might indicate that cation e ects resulted in neutralization of the zeta potential and are in accor-dance with Schulze Hardy's law(Klute and Neis,1976).3.5.Flocculation of various suspended solidsThe spectrum of¯occulating ability for various sus-pended solids in aqueous solution is shown in Table3.The¯occulant¯occulated e ciently all the tested solid suspensions,with some variations in activity.The sus-pensions of kaolin,active carbon and soil solid could be ¯occulated e ectively in the presence of Ca2 ,the¯oc-culating activities in suspensions containing3.7mg/l of biopolymer were8.5,3.0,1.4,respectively.The Ca(OH)2 and Mg(OH)2suspensions could be¯occulated by the addition of biopolymer alone.The suspensions of alu-minum oxide,cellulose,CM cellulose and yeast were better¯occulated by the¯occulant in the neutral pH ranges.Recently Yokoi and coworkers(Yokoi et al.,1995, 1996)have demonstrated that poly(c-glutamic acid) produced by Bacillus sp.PY-90and Bacillus subtillis IFO3335had a high¯occulating activity.However,high ¯occulating PGA produced by B.licheniformis species has never been reported.The facts that the¯occulant from B.licheniformis CCRC12826has a¯occulating activity for a wide range of organic and inorganic compounds suggest that such a¯occulant could be ap-plied in many industries.It is anticipated that PGA will be utilized not only in the areas of wastewater treatment but also drinking water processing and downstream processing in the food and fermentation industry be-cause of its harmlessness toward humans and the envi-ronment.AcknowledgementsThis work was supported partially by Grant NSC89-2313-B-212-007from National Science Council of ROC.ReferencesBradford,M.M.,1976.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal.Biochem.72,248±254. Chaplin,M.F.,Kennedy,J.F.,1986.Carbohydrate Analysis.IRL Press,Washington,DC,pp.2±5.Dear®eld,K.L.,Abermathy,C.O.,1988.Acrylamide:its metabolism, developmental and reproductive e ects,genotoxicity,and carcino-genicity.Mutant.Res.195,45±77.Frideman,T.E.,Haugen,G.E.,1943.The determination of keto acids in blood and urine.Pyruvic acid,II.J.Biol.Chem.147,415±442. Gutcho,S.,1977.Waste Treatment with Polyelectrolytes and other Flocculants.Noyes Data Corp.,Park Ridge,NJ,pp.1±37. Klute,R.,Neis,U.,1976.Stability of colloidal kaolinite suspensions in the presence of soluble organic compounds.In:M.Ker(Ed.), Proceedings of the International Conference,Colloid Interface Science,vol.4,50th ed.p.113.Kurane,R.,Takeda,K.,Suzuki,T.,1986.Screening for and characteristics of microbial¯occulant.Agric.Biol.Chem.50, 2301±2307.Kurane,R.,Nohata,Y.,1991.Microbial¯occulation of waste liquids and oil emulsion by a bio¯occulant from Alcaligenes latus.Agric.Biol.Chem.55,1127±1129.Kurane,R.,Hatamochi,K.,Kakuno,T.,Kiyohara,M.,Hirano,M., Taniguchi,Y.,1994.Production of a bio¯occulant by RhodococcusTable3The¯occulating activity of¯occulant produced by B.licheniformisCCRC12826on various suspended solidsVarious solid suspensions Flocculating activity(1/OD)Kaoline clay8.5Active carbon 3.0Soil solid 1.4Ca(OH)211Mg(OH)2 5.0Aluminum oxide a 6.1Cellulose powder a 3.0C.M.cellulose a 2.6S.serevisiae a 4.6B.circulans a 2.8a Activity was measured at neutral pH range.I.L.Shih et al./Bioresource Technology78(2001)267±272271erythropolis S-1grown on alcohols.Biosci.Biotech.Biochem.58, 428±429.Lee,S.H.,Lee,S.O.,Jang,K.L.,Lee,T.H.,1995.Microbial¯occulant from Arcuadendron sp.TS-49.Biotechnol.Lett.17,95±100. Leonard,C.G.,Housewright,R.D.,Thorne,C.B.,1958.E ects of some metallic ions on glytamyl polypeptide synthesis by Bacillus subtilis.J.Bacteriol.76,499±503.McComb,E.A.,McCreasy,R.M.,1957.Determination of acetyl in pectin and in acetylated carbohydrated polymers.Anal.Chem.29, 819±821.Michaels,A.S.,1954.Aggregation of suspensions by polyelectrolytes.Ind.Eng.Chem.46,1485.Nakamura,J.,Miyashiro,S.,Hirose,Y.,1976.Conditions for production of microbial cell¯occulant by Aspergillus sojae AJ7002.Agric.Biol.Chem.40,1341±1347.Stewart,J.M.,Young,J.D.,boratory techniques.In:Solid Phase Peptide Synthesis,second ed.Pierce Chemical Co,Rockford, IL,p.104(Chapter2).Suh,H.H.,Kwon,G.S.,Lee,C.H.,Kim,H.S.,Oh,H.M.,Yoon,B.D., 1997.Characterization of bio¯occulant produced by Bacillus sp.DP-152.J.Ferment.Bioeng.84,108±112.Takagi,H.,Kadowaki,K.,1985.Flocculant production by Pacilomy-ces sp.Taxonomic studies and culture conditions for production.Agric.Biol.Chem.49,3151±3157.Takeda,M.,Kurane,R.,Koizumi,J.,Nakamura,I.,1991.A protein biofocculant produced by Rhodococcus erythropolis.Agric.Biol.Chem.55,2663±2664.Takeda,M.,Koizumi,J.,Matsuoka,H.,Hikuma,M.,1992.Factorsa ecting the activity of a protein bio¯occulant produced byNocardia amarae.J.Ferment.Bioeng.74,408±409.Toeda,K.,Kurane,R.,1991.Microbial¯occulant from Alcaligenes cupidas KT201.Agric.Biol.Chem.55,2793±2799. Vanhorick,M.,Moens,W.,1983.Carcinogen of acrylamide.Carci-nogenesis4,1459±1463.Yokoi,H.,Natsuda,O.,Hirose,J.,Hayashi,S.,Takasaki,Y.,1995.Characteristics of a biopolymer¯occulant produced by Bacillus sp.PY-90.J.Ferment.Bioeng.79,378±380.Yokoi,H.,Arima,T.,Hirose,J.,Hayashi,S.,Takasaki,Y.,1996.Flocculation properties of poly(gamma-glutamic acid)produced by Bacillus subtilis.J.Ferment.Bioeng.82,84±87.Wang,Z.,Wang,K.,Xie,Y.,1995.Bio¯occulant-producing micro-organisms.Acta Microbiol.Sin.35(2),121±129.272I.L.Shih et al./Bioresource Technology78(2001)267±272。
Sensorless vector control of induction motors at very low speed using a nonlinear inverter model and
Sensorless Vector Control of Induction Motors at Very Low Speed Using a Nonlinear Inverter Model and Parameter IdentificationJoachim Holtz,Fellow,IEEE,and Juntao QuanAbstract—The performance of vector-controlled induction motor drives without speed sensor is generally poor at very low speed.The reasons are offset and drift components in the acquired feedback signals,voltage distortions caused by the non-linear behavior of the switching converter,and the increased sensitivity against model parameter mismatch.New modeling and identification techniques are proposed to overcome these problems.A pure integrator is employed for stator flux estima-tion which permits high-estimation pensation of the drift components is done by offset identification.The nonlinear voltage distortions are corrected by a self-adjusting inverter model.A further improvement is a novel method for on-line adaptation of the stator resistance.Experiments demonstrate smooth steady-state operation and high dynamic performance at extremely low speed.Index Terms—Induction motor,low-speed operation,parameter identification,sensorless control,vector control.I.I NTRODUCTIONC ONTROLLED induction motor drives without speedsensor have developed as a mature technology in the past few years.However,their performance at very low speed is poor.The main reasons are the limited accuracy of stator voltage acquisition,the presence of offset and drift compo-nents in the acquired voltage signals,their limited bandwidth, offsets and unbalances in the current signals,and the increased sensitivity against model parameter mismatch.These deficiencies degrade the accuracy of flux estimation at low speed.The dynamic performance of a sensorless drive then deteriorates.Sustained operation at very low speed becomes im-possible as ripple components appear in the machine torque and the speed starts oscillating,eventually leading to instable oper-ation of the system.Paper IPCSD02–025,presented at the2001Industry Applications Society Annual Meeting,Chicago,IL,September30–October5,and approved for publication in the IEEE T RANSACTIONS ON I NDUSTRY A PPLICATIONS by the Industrial Drives Committee of the IEEE Industry Applications Society. Manuscript submitted for review October15,2001and released for publication May10,2002.J.Holtz is with the Electrical Machines and Drives Group,University of Wup-pertal,42097Wuppertal,Germany(e-mail:j.holtz@).J.Quan is with the Danaher Motion Group,Kollmorgen-Seidel,Duesseldorf, Germany(e-mail:jquan@).Publisher Item Identifier10.1109/TIA.2002.800779.II.S OURCES OF I NACCURACY AND I NSTABILITYA.Estimation of the Flux Linkage VectorMost sensorless control schemes rely directly or indirectly on the estimation of the stator flux linkagevectoris the stator resistance.Time is normalizedasis the nominal stator frequency[3].The addedsymbol in (1)represents all disturbances such as offsets,unbalances,and other errors that are contained in the estimated inducedvoltage(2)where is the coupling factor of the rotorwindings,is the total leakage flux vector.The estimation of one of the flux vectors according to(1)or (2)requires performing an integration in real time.The use of a pure integrator has not been reported in the literature.The reason is that an integrator has an infinite gain at zero frequency.The unavoidable offsets contained in the integrator input then make its output gradually drift away beyond limits.Therefore,instead of an integrator,a low-pass filter usually serves as a substitute.A low-pass filter has a finite dc gain which eases the drift problem, although drift is not fully avoided.However,a low-pass filter in-troduces severe phase angle and amplitude errors at frequencies around its corner frequency,and even higher errors at lower fre-quencies.Its corner frequency is normally set to0.5–2Hz,de-pending on the existing amount of offset.The drive performance degrades below stator frequencies2–3times this value;the drive becomes instable at speed values that correspond to the corner frequency.Different ways of compensating the amplitude and phase-angle errors at low frequencies have been proposed[4]–[7].0093-9994/02$17.00©2002IEEEOhtani [4]reconstructs the phase-angle and amplitude error pro-duced by the low-pass filter.A load-dependent flux vector refer-ence is synthesized for this purpose.This signal is transformed to stator coordinates and then passed through a second low-pass filter having the same time constant.The resulting error vector is added to the erroneous flux estimate.Although the benefits of this method are not explicitly documented in [4],improved performance should be expected in an operating range around the corner frequency of the low-pass filter.With a view to improving the low-speed performance of flux estimation,Shin et al.[5]adjust the corner frequency of the low-pass filter in proportion to the stator frequency,while com-pensating the phase and gain errors by their respective steady-state values.It was not demonstrated,though,that dynamic op-eration at very low frequency is improved.Hu and Wu [6]try to force the stator flux vector onto a circular trajectory by propor-tional plus integral (PI)control.While this can provide a correct result in the steady state,it is erroneous at transient operation and also exhibits a large error at startup.A practical application of this method has not been reported;our investigations show loss of field orientation following transients.B.Acquisition of the Stator VoltagesThe induced voltage,which is the signal to be integrated for flux vector estimation,is obtained as the difference between the stator voltage and the resistive voltage drop across the ma-chine windings.When a voltage-source inverter (VSI)is used to feed the machine,the stator voltages are formed by pulse trains having a typical rise time of 2–10kV/,whereis the fundamental componentofcaused by the switching characteristics of the inverter.C.Acquisition of the Stator CurrentsThe stator currents are usually measured by two Hall sensors.They are acquired as analog signals,which are subsequently digitized using A/D converters.The sources of errors in this process are dc offsets and gain unbalances in the analog signal channels [9].After the transformation of the current signals to synchronous coordinates,dc offsets generate ac ripple compo-nents of fundamental frequency,while gain unbalances produce elliptic current trajectories instead of circular trajectories.The disturbance in the latter case is a signal of twice the fundamentalfrequency.Fig.1.Effect of a dc offset in one of the current signals on the performance of a vector-controlled drivesystem.Fig.2.Effect of a gain unbalance between the acquired current signals on the performance of a vector-controlled drive.The following oscillograms demonstrate the effect of such disturbances on the performance of a vector-controlled drive system.The respective disturbances are intentionally intro-duced,for better visibility at a higher signal level than would normally be expected in a practical implementation.Fig.1shows the effect of 5%dc offset in one of the current signals on the no-load waveform ofthe.The drive is operated is at astator frequency of 2Hz.The transformed current signals gen-erate oscillations in the torque-producing current .Resulting from this are torque pulsations of 0.06nominal value,and cor-responding oscillations in the speedsignal,where isthe power factor of the motor.Fig.2shows the same signals under the influence of 5%gain unbalance between the two current channels.Oscillations of twice the stator frequency are generated in the torque-producing current,and also in the speed signal.D.Estimation of the Stator ResistanceAnother severe issue,in addition to the integration problem and to the nonlinear behavior of the inverter,is the mismatch be-tween the machine parameters and the respective model param-eters.In particular,adjusting the stator resistanceHOLTZ AND QUAN:SENSORLESS VECTOR CONTROL OF INDUCTION MOTORS1089Fig.3.Forward characteristics of the power devices.flux estimation,and for stable operation at very low speed.The actual valueofand an averagedifferentialresistance [12].The variations with temperatureof the thresholdvoltageof about equal magnitude to all the threephases,and it is the directions of the respective phase currents that determine their signs.The device thresholdvoltage(4)where.Thesectorindicator is a unity vector that indicates the re-spectivein thecomplex plane.The locations are determined by the respective signs of the three phase currents in (3),or,in other words,by a maximumof.The referencesignalof the stator voltagevectoris less than its referencevalue,and of the resis-tive voltage drops of the power devicesthrough1090IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS,VOL.38,NO.4,JULY/AUGUST2002(a)(b)(c)Fig.4.Effect at PWM of the forward voltagesuof the power semiconductors.(a)Switching state S.(c)Switching state S);the dotted linesindicate the transitions at which the signs of the respective phase currents change.Notethatis the resulting threshold voltage vector.We have,therefore,from (4),the unusualrelationshipis one parameter of the invertermodel.It is determined during a self-commissioning process from the distortions of the reference voltagevectorand of the reference voltage vector are acquiredwhile using the current controllers to inject sinusoidal currents of very low frequency into the stator windings.In such condi-tion,the machine impedance is dominated by the stator resis-tance.The stator voltages are then proportional to the stator cur-rents.Any deviation from a sinewave of the reference voltages that control the pulsewidth modulator are,therefore,caused by the inverter.As an example,an oscillogram of the distorted referencevoltagewaveformsand ,measured at sinusoidal currents ofmagnitude ,is shown in Fig.7.The amplitude of the fundamental voltage is very low which is owed to the low frequency of operation.The distortions of the voltage waveforms in Fig.7are,therefore,fairly high.They are predominantly caused by the dead-time effect of the ing such distorted voltages to represent the stator voltage signal in a stator flux estimator would lead to stability problems at low speed.Accurate inverter dead-time compensation [13]is,therefore,mandatory for high-performance applications.Fig.8shows the same components of the reference voltagevectoraccording to (4);the locationsofare shown in Fig.4.It follows from (4)that both the larger step change and the amplitudeofhave the magnitude4/3from thewaveformof(or )in Fig.8appears quite inaccurate.A better method is subtracting the fundamentalcomponent from,e.g.,,which then yields a square-wave-like,stepped waveform as shown in Fig.9.The fundamental component iseasily extracted from a set of synchronous samplesofby fast Fourier transform.The differential resistance of the powerdevices,in (6),es-tablishes a linear relation between the load current and its in-fluence on the inverter voltage.Functionally,it adds to the re-sistance)is estimated by an online tuning process described inSection III-D.HOLTZ AND QUAN:SENSORLESS VECTOR CONTROL OF INDUCTION MOTORS1091(a)(b)Fig.6.Effect of inverter nonlinearity.The trajectory u represents the average stator voltage (switching harmonics excluded).(a)At motoring.(b)Atregeneration.Fig.7.Effect of inverter dead time on the components of the voltage vectoruas in Fig.7;inverteroperated with dead-time compensation.C.Stator Flux EstimationThe inverter model (6)is used to compensate the nonlinear distortions introduced by the power devices of the inverter.The model estimates the stator voltagevector(8)is the estimated effective offset voltage vector,while is theestimated stator field angle.The offset voltagevectorin (7)is determined such that the estimated stator fluxvector rotates close to a circular trajectory in the steady state,which follows from (7)and from the right-hand side of (8).To enable the identificationofin (8),the stator field angle is estimatedas(9)as illustrated in the right portion of Fig.10.The magnitude of the stator flux linkage vector is then obtainedas1092IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS,VOL.38,NO.4,JULY/AUGUST2002Fig.10.Signal flow graph of the inverter model and the stator flux estimator.The gain constantserve this purpose in a satisfactorymanner.The stator frequency signal is computed byis determined,for instance,with reference to[2]of the stator current,as shown in Fig.11.We haveand,consequently,.Of the superscripts,component of the vector product of the statorvoltage and current vectors.The system equation,for example given in[3],isHOLTZ AND QUAN:SENSORLESS VECTOR CONTROL OF INDUCTION MOTORS1093Fig.12.Signal flow graph of the stator resistance estimator.wherecomponent of all terms in(19)and assumingfieldorientation,,wehave10toa n d=!=wp w wp p f p p w1094IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS,VOL.38,NO.4,JULY/AUGUST2002Fig.16.Identification of the stator resistance,demonstrated by a30%stepincrease of the resistance value.Fig.17.Reversal of speed between the set-point values w=60:04;torqueis constant at50%nominal value.the speed is negative.Finally,the performance of the stator re-sistance identification scheme is demonstrated in Fig.17.Thestator resistance is increased by30%in a step-change fashion.The disturbance causes a sudden deviation from the correct fieldangle,which produces a wrong value.The new value ofHOLTZ AND QUAN:SENSORLESS VECTOR CONTROL OF INDUCTION MOTORS1095 Juntao Quan was born in Jiangxi,China,in1964.Hereceived the B.Eng.degree from Jiangxi PolytechnicCollege,Nanchang University,Nanchang,China,the M.Eng.degree from Northeast-Heavy MechanicInstitute,Yanshan University,Qinhuangdao,China,and the Ph.D.degree from Wuppertal University,Wuppertal,Germany,in1983,1989,and2002,respectively,all in electrical engineering.He was an Assistant Electrical Engineer for threeyears at the Nanchang Bus Factory,Nanchang,China.From1989to1994,he was a Lecturer at YanshanUniversity.During this time,he also worked on various projects for applicationsof power electronics.In1995,he joined the Electrical Machines and Drives Lab-oratory,Wuppertal University,where he worked and studied toward the Ph.D.degree.In June2000,he joined the Danaher Motion Group,Kollmorgen-Seidel,Duesseldorf,Germany.His main interests are in the areas of adjustable-speeddrives,microprocessor-embedded real-time control,power electronics applica-tions,and advanced motion control.。
Glider Flying Handbook说明书
Glider Flying Handbook2013U.S. Department of TransportationFEDERAL AVIATION ADMINISTRATIONFlight Standards Servicei iPrefaceThe Glider Flying Handbook is designed as a technical manual for applicants who are preparing for glider category rating and for currently certificated glider pilots who wish to improve their knowledge. Certificated flight instructors will find this handbook a valuable training aid, since detailed coverage of aeronautical decision-making, components and systems, aerodynamics, flight instruments, performance limitations, ground operations, flight maneuvers, traffic patterns, emergencies, soaring weather, soaring techniques, and cross-country flight is included. Topics such as radio navigation and communication, use of flight information publications, and regulations are available in other Federal Aviation Administration (FAA) publications.The discussion and explanations reflect the most commonly used practices and principles. Occasionally, the word “must” or similar language is used where the desired action is deemed critical. The use of such language is not intended to add to, interpret, or relieve a duty imposed by Title 14 of the Code of Federal Regulations (14 CFR). Persons working towards a glider rating are advised to review the references from the applicable practical test standards (FAA-G-8082-4, Sport Pilot and Flight Instructor with a Sport Pilot Rating Knowledge Test Guide, FAA-G-8082-5, Commercial Pilot Knowledge Test Guide, and FAA-G-8082-17, Recreational Pilot and Private Pilot Knowledge Test Guide). Resources for study include FAA-H-8083-25, Pilot’s Handbook of Aeronautical Knowledge, FAA-H-8083-2, Risk Management Handbook, and Advisory Circular (AC) 00-6, Aviation Weather For Pilots and Flight Operations Personnel, AC 00-45, Aviation Weather Services, as these documents contain basic material not duplicated herein. All beginning applicants should refer to FAA-H-8083-25, Pilot’s Handbook of Aeronautical Knowledge, for study and basic library reference.It is essential for persons using this handbook to become familiar with and apply the pertinent parts of 14 CFR and the Aeronautical Information Manual (AIM). The AIM is available online at . The current Flight Standards Service airman training and testing material and learning statements for all airman certificates and ratings can be obtained from .This handbook supersedes FAA-H-8083-13, Glider Flying Handbook, dated 2003. Always select the latest edition of any publication and check the website for errata pages and listing of changes to FAA educational publications developed by the FAA’s Airman Testing Standards Branch, AFS-630.This handbook is available for download, in PDF format, from .This handbook is published by the United States Department of Transportation, Federal Aviation Administration, Airman Testing Standards Branch, AFS-630, P.O. Box 25082, Oklahoma City, OK 73125.Comments regarding this publication should be sent, in email form, to the following address:********************************************John M. AllenDirector, Flight Standards Serviceiiii vAcknowledgmentsThe Glider Flying Handbook was produced by the Federal Aviation Administration (FAA) with the assistance of Safety Research Corporation of America (SRCA). The FAA wishes to acknowledge the following contributors: Sue Telford of Telford Fishing & Hunting Services for images used in Chapter 1JerryZieba () for images used in Chapter 2Tim Mara () for images used in Chapters 2 and 12Uli Kremer of Alexander Schleicher GmbH & Co for images used in Chapter 2Richard Lancaster () for images and content used in Chapter 3Dave Nadler of Nadler & Associates for images used in Chapter 6Dave McConeghey for images used in Chapter 6John Brandon (www.raa.asn.au) for images and content used in Chapter 7Patrick Panzera () for images used in Chapter 8Jeff Haby (www.theweatherprediction) for images used in Chapter 8National Soaring Museum () for content used in Chapter 9Bill Elliot () for images used in Chapter 12.Tiffany Fidler for images used in Chapter 12.Additional appreciation is extended to the Soaring Society of America, Inc. (), the Soaring Safety Foundation, and Mr. Brad Temeyer and Mr. Bill Martin from the National Oceanic and Atmospheric Administration (NOAA) for their technical support and input.vv iPreface (iii)Acknowledgments (v)Table of Contents (vii)Chapter 1Gliders and Sailplanes ........................................1-1 Introduction....................................................................1-1 Gliders—The Early Years ..............................................1-2 Glider or Sailplane? .......................................................1-3 Glider Pilot Schools ......................................................1-4 14 CFR Part 141 Pilot Schools ...................................1-5 14 CFR Part 61 Instruction ........................................1-5 Glider Certificate Eligibility Requirements ...................1-5 Common Glider Concepts ..............................................1-6 Terminology...............................................................1-6 Converting Metric Distance to Feet ...........................1-6 Chapter 2Components and Systems .................................2-1 Introduction....................................................................2-1 Glider Design .................................................................2-2 The Fuselage ..................................................................2-4 Wings and Components .............................................2-4 Lift/Drag Devices ...........................................................2-5 Empennage .....................................................................2-6 Towhook Devices .......................................................2-7 Powerplant .....................................................................2-7 Self-Launching Gliders .............................................2-7 Sustainer Engines .......................................................2-8 Landing Gear .................................................................2-8 Wheel Brakes .............................................................2-8 Chapter 3Aerodynamics of Flight .......................................3-1 Introduction....................................................................3-1 Forces of Flight..............................................................3-2 Newton’s Third Law of Motion .................................3-2 Lift ..............................................................................3-2The Effects of Drag on a Glider .....................................3-3 Parasite Drag ..............................................................3-3 Form Drag ...............................................................3-3 Skin Friction Drag ..................................................3-3 Interference Drag ....................................................3-5 Total Drag...................................................................3-6 Wing Planform ...........................................................3-6 Elliptical Wing ........................................................3-6 Rectangular Wing ...................................................3-7 Tapered Wing .........................................................3-7 Swept-Forward Wing ..............................................3-7 Washout ..................................................................3-7 Glide Ratio .................................................................3-8 Aspect Ratio ............................................................3-9 Weight ........................................................................3-9 Thrust .........................................................................3-9 Three Axes of Rotation ..................................................3-9 Stability ........................................................................3-10 Flutter .......................................................................3-11 Lateral Stability ........................................................3-12 Turning Flight ..............................................................3-13 Load Factors .................................................................3-13 Radius of Turn ..........................................................3-14 Turn Coordination ....................................................3-15 Slips ..........................................................................3-15 Forward Slip .........................................................3-16 Sideslip .................................................................3-17 Spins .........................................................................3-17 Ground Effect ...............................................................3-19 Chapter 4Flight Instruments ...............................................4-1 Introduction....................................................................4-1 Pitot-Static Instruments ..................................................4-2 Impact and Static Pressure Lines................................4-2 Airspeed Indicator ......................................................4-2 The Effects of Altitude on the AirspeedIndicator..................................................................4-3 Types of Airspeed ...................................................4-3Table of ContentsviiAirspeed Indicator Markings ......................................4-5 Other Airspeed Limitations ........................................4-6 Altimeter .....................................................................4-6 Principles of Operation ...........................................4-6 Effect of Nonstandard Pressure andTemperature............................................................4-7 Setting the Altimeter (Kollsman Window) .............4-9 Types of Altitude ......................................................4-10 Variometer................................................................4-11 Total Energy System .............................................4-14 Netto .....................................................................4-14 Electronic Flight Computers ....................................4-15 Magnetic Compass .......................................................4-16 Yaw String ................................................................4-16 Inclinometer..............................................................4-16 Gyroscopic Instruments ...............................................4-17 G-Meter ........................................................................4-17 FLARM Collision Avoidance System .........................4-18 Chapter 5Glider Performance .............................................5-1 Introduction....................................................................5-1 Factors Affecting Performance ......................................5-2 High and Low Density Altitude Conditions ...........5-2 Atmospheric Pressure .............................................5-2 Altitude ...................................................................5-3 Temperature............................................................5-3 Wind ...........................................................................5-3 Weight ........................................................................5-5 Rate of Climb .................................................................5-7 Flight Manuals and Placards ..........................................5-8 Placards ......................................................................5-8 Performance Information ...........................................5-8 Glider Polars ...............................................................5-8 Weight and Balance Information .............................5-10 Limitations ...............................................................5-10 Weight and Balance .....................................................5-12 Center of Gravity ......................................................5-12 Problems Associated With CG Forward ofForward Limit .......................................................5-12 Problems Associated With CG Aft of Aft Limit ..5-13 Sample Weight and Balance Problems ....................5-13 Ballast ..........................................................................5-14 Chapter 6Preflight and Ground Operations .......................6-1 Introduction....................................................................6-1 Assembly and Storage Techniques ................................6-2 Trailering....................................................................6-3 Tiedown and Securing ................................................6-4Water Ballast ..............................................................6-4 Ground Handling........................................................6-4 Launch Equipment Inspection ....................................6-5 Glider Preflight Inspection .........................................6-6 Prelaunch Checklist ....................................................6-7 Glider Care .....................................................................6-7 Preventive Maintenance .............................................6-8 Chapter 7Launch and Recovery Procedures and Flight Maneuvers ............................................................7-1 Introduction....................................................................7-1 Aerotow Takeoff Procedures .........................................7-2 Signals ........................................................................7-2 Prelaunch Signals ....................................................7-2 Inflight Signals ........................................................7-3 Takeoff Procedures and Techniques ..........................7-3 Normal Assisted Takeoff............................................7-4 Unassisted Takeoff.....................................................7-5 Crosswind Takeoff .....................................................7-5 Assisted ...................................................................7-5 Unassisted...............................................................7-6 Aerotow Climb-Out ....................................................7-6 Aerotow Release.........................................................7-8 Slack Line ...................................................................7-9 Boxing the Wake ......................................................7-10 Ground Launch Takeoff Procedures ............................7-11 CG Hooks .................................................................7-11 Signals ......................................................................7-11 Prelaunch Signals (Winch/Automobile) ...............7-11 Inflight Signals ......................................................7-12 Tow Speeds ..............................................................7-12 Automobile Launch ..................................................7-14 Crosswind Takeoff and Climb .................................7-14 Normal Into-the-Wind Launch .................................7-15 Climb-Out and Release Procedures ..........................7-16 Self-Launch Takeoff Procedures ..............................7-17 Preparation and Engine Start ....................................7-17 Taxiing .....................................................................7-18 Pretakeoff Check ......................................................7-18 Normal Takeoff ........................................................7-19 Crosswind Takeoff ...................................................7-19 Climb-Out and Shutdown Procedures ......................7-19 Landing .....................................................................7-21 Gliderport/Airport Traffic Patterns and Operations .....7-22 Normal Approach and Landing ................................7-22 Crosswind Landing ..................................................7-25 Slips ..........................................................................7-25 Downwind Landing ..................................................7-27 After Landing and Securing .....................................7-27viiiPerformance Maneuvers ..............................................7-27 Straight Glides ..........................................................7-27 Turns.........................................................................7-28 Roll-In ...................................................................7-29 Roll-Out ................................................................7-30 Steep Turns ...........................................................7-31 Maneuvering at Minimum Controllable Airspeed ...7-31 Stall Recognition and Recovery ...............................7-32 Secondary Stalls ....................................................7-34 Accelerated Stalls .................................................7-34 Crossed-Control Stalls ..........................................7-35 Operating Airspeeds .....................................................7-36 Minimum Sink Airspeed ..........................................7-36 Best Glide Airspeed..................................................7-37 Speed to Fly ..............................................................7-37 Chapter 8Abnormal and Emergency Procedures .............8-1 Introduction....................................................................8-1 Porpoising ......................................................................8-2 Pilot-Induced Oscillations (PIOs) ..............................8-2 PIOs During Launch ...................................................8-2 Factors Influencing PIOs ........................................8-2 Improper Elevator Trim Setting ..............................8-3 Improper Wing Flaps Setting ..................................8-3 Pilot-Induced Roll Oscillations During Launch .........8-3 Pilot-Induced Yaw Oscillations During Launch ........8-4 Gust-Induced Oscillations ..............................................8-5 Vertical Gusts During High-Speed Cruise .................8-5 Pilot-Induced Pitch Oscillations During Landing ......8-6 Glider-Induced Oscillations ...........................................8-6 Pitch Influence of the Glider Towhook Position ........8-6 Self-Launching Glider Oscillations During Powered Flight ...........................................................8-7 Nosewheel Glider Oscillations During Launchesand Landings ..............................................................8-7 Tailwheel/Tailskid Equipped Glider Oscillations During Launches and Landings ..................................8-8 Aerotow Abnormal and Emergency Procedures ............8-8 Abnormal Procedures .................................................8-8 Towing Failures........................................................8-10 Tow Failure With Runway To Land and Stop ......8-11 Tow Failure Without Runway To Land BelowReturning Altitude ................................................8-11 Tow Failure Above Return to Runway Altitude ...8-11 Tow Failure Above 800' AGL ..............................8-12 Tow Failure Above Traffic Pattern Altitude .........8-13 Slack Line .................................................................8-13 Ground Launch Abnormal and Emergency Procedures ....................................................................8-14 Abnormal Procedures ...............................................8-14 Emergency Procedures .............................................8-14 Self-Launch Takeoff Emergency Procedures ..............8-15 Emergency Procedures .............................................8-15 Spiral Dives ..................................................................8-15 Spins .............................................................................8-15 Entry Phase ...............................................................8-17 Incipient Phase .........................................................8-17 Developed Phase ......................................................8-17 Recovery Phase ........................................................8-17 Off-Field Landing Procedures .....................................8-18 Afterlanding Off Field .............................................8-20 Off-Field Landing Without Injury ........................8-20 Off-Field Landing With Injury .............................8-20 System and Equipment Malfunctions ..........................8-20 Flight Instrument Malfunctions ................................8-20 Airspeed Indicator Malfunctions ..........................8-21 Altimeter Malfunctions .........................................8-21 Variometer Malfunctions ......................................8-21 Compass Malfunctions .........................................8-21 Glider Canopy Malfunctions ....................................8-21 Broken Glider Canopy ..........................................8-22 Frosted Glider Canopy ..........................................8-22 Water Ballast Malfunctions ......................................8-22 Retractable Landing Gear Malfunctions ..................8-22 Primary Flight Control Systems ...............................8-22 Elevator Malfunctions ..........................................8-22 Aileron Malfunctions ............................................8-23 Rudder Malfunctions ............................................8-24 Secondary Flight Controls Systems .........................8-24 Elevator Trim Malfunctions .................................8-24 Spoiler/Dive Brake Malfunctions .........................8-24 Miscellaneous Flight System Malfunctions .................8-25 Towhook Malfunctions ............................................8-25 Oxygen System Malfunctions ..................................8-25 Drogue Chute Malfunctions .....................................8-25 Self-Launching Gliders ................................................8-26 Self-Launching/Sustainer Glider Engine Failure During Takeoff or Climb ..........................................8-26 Inability to Restart a Self-Launching/SustainerGlider Engine While Airborne .................................8-27 Self-Launching Glider Propeller Malfunctions ........8-27 Self-Launching Glider Electrical System Malfunctions .............................................................8-27 In-flight Fire .............................................................8-28 Emergency Equipment and Survival Gear ...................8-28 Survival Gear Checklists ..........................................8-28 Food and Water ........................................................8-28ixClothing ....................................................................8-28 Communication ........................................................8-29 Navigation Equipment ..............................................8-29 Medical Equipment ..................................................8-29 Stowage ....................................................................8-30 Parachute ..................................................................8-30 Oxygen System Malfunctions ..................................8-30 Accident Prevention .....................................................8-30 Chapter 9Soaring Weather ..................................................9-1 Introduction....................................................................9-1 The Atmosphere .............................................................9-2 Composition ...............................................................9-2 Properties ....................................................................9-2 Temperature............................................................9-2 Density ....................................................................9-2 Pressure ...................................................................9-2 Standard Atmosphere .................................................9-3 Layers of the Atmosphere ..........................................9-4 Scale of Weather Events ................................................9-4 Thermal Soaring Weather ..............................................9-6 Thermal Shape and Structure .....................................9-6 Atmospheric Stability .................................................9-7 Air Masses Conducive to Thermal Soaring ...................9-9 Cloud Streets ..............................................................9-9 Thermal Waves...........................................................9-9 Thunderstorms..........................................................9-10 Lifted Index ..........................................................9-12 K-Index .................................................................9-12 Weather for Slope Soaring .......................................9-14 Mechanism for Wave Formation ..............................9-16 Lift Due to Convergence ..........................................9-19 Obtaining Weather Information ...................................9-21 Preflight Weather Briefing........................................9-21 Weather-ReIated Information ..................................9-21 Interpreting Weather Charts, Reports, andForecasts ......................................................................9-23 Graphic Weather Charts ...........................................9-23 Winds and Temperatures Aloft Forecast ..............9-23 Composite Moisture Stability Chart .....................9-24 Chapter 10Soaring Techniques ..........................................10-1 Introduction..................................................................10-1 Thermal Soaring ...........................................................10-2 Locating Thermals ....................................................10-2 Cumulus Clouds ...................................................10-2 Other Indicators of Thermals ................................10-3 Wind .....................................................................10-4 The Big Picture .....................................................10-5Entering a Thermal ..............................................10-5 Inside a Thermal.......................................................10-6 Bank Angle ...........................................................10-6 Speed .....................................................................10-6 Centering ...............................................................10-7 Collision Avoidance ................................................10-9 Exiting a Thermal .....................................................10-9 Atypical Thermals ..................................................10-10 Ridge/Slope Soaring ..................................................10-10 Traps ......................................................................10-10 Procedures for Safe Flying .....................................10-12 Bowls and Spurs .....................................................10-13 Slope Lift ................................................................10-13 Obstructions ...........................................................10-14 Tips and Techniques ...............................................10-15 Wave Soaring .............................................................10-16 Preflight Preparation ...............................................10-17 Getting Into the Wave ............................................10-18 Flying in the Wave .................................................10-20 Soaring Convergence Zones ...................................10-23 Combined Sources of Updrafts ..............................10-24 Chapter 11Cross-Country Soaring .....................................11-1 Introduction..................................................................11-1 Flight Preparation and Planning ...................................11-2 Personal and Special Equipment ..................................11-3 Navigation ....................................................................11-5 Using the Plotter .......................................................11-5 A Sample Cross-Country Flight ...............................11-5 Navigation Using GPS .............................................11-8 Cross-Country Techniques ...........................................11-9 Soaring Faster and Farther .........................................11-11 Height Bands ..........................................................11-11 Tips and Techniques ...............................................11-12 Special Situations .......................................................11-14 Course Deviations ..................................................11-14 Lost Procedures ......................................................11-14 Cross-Country Flight in a Self-Launching Glider .....11-15 High-Performance Glider Operations and Considerations ............................................................11-16 Glider Complexity ..................................................11-16 Water Ballast ..........................................................11-17 Cross-Country Flight Using Other Lift Sources ........11-17 Chapter 12Towing ................................................................12-1 Introduction..................................................................12-1 Equipment Inspections and Operational Checks .........12-2 Tow Hook ................................................................12-2 Schweizer Tow Hook ...........................................12-2x。
卡洛洛·加卜梭·控制S.p.A. CPA家族 CPA300V DS ENG 2020年7月22日 无
CPA FamilyContactless power analyzersDescriptionCPA is a family of power analyzers and current transducers for ac 1-phase or dc installation monitoring, thanks to Hall effect sensing.Current is measured with no contact with the copper wire.The comprehensive set of measured variables allow this device to be used to monitor photovoltaic installations, industrial processes, battery charging systems.Benefits• Flexible solution. The instrument allows users to monitor both ac and dc system with the same device.• Fast connection. ac or dc current sensing with no need to cut and join the cable.• Reliability. The instrument is equipped with a Modbus/RTU communication port by RS485 connection.• Complete monitoring. Depending on the model, the instrument provides a comprehensive range of monitored variables (V, A,W, var, VA, kWh, PF, HZ, THD) or limited to current variables (A, Amin, Amax, Ah).• Wide range of device mounting types. The instrument can be mounted in four different ways (either DIN rail or panel mounting, vertical or horizontal) to match different installation constraints.• Easy programming. Plug’n play set-up by means of CARLO GAVAZZI UCS (Universal Configuration Software).• Integrated solution. The instrument is compatible with both UWP 3.0 and VMU-C EM solutions for energy monitoring.ApplicationsCPA power analyzers are the ideal solution for those applications which are beyond standard ac monitoring.Given their capability of working both at different frequency ranges, they match the needs of dc applications (battery charging, photovoltaic monitoring), of ac applications with high crest factor (UPS, variable frequency drives) and standard 1-phase ac installations.Main functions• Compatible with VMU-C EM and UWP 3.0• Configurable by means of UCS (Universal Configuration Software) solution • Hall effect sensingCPA system architecture for ac systemCPA operating principles for ac systemsCPA is a power analyzer, measuring current with contactless Hall effect sensing and voltage with shunt based technology.Power, power factor, energy, frequency and harmonic distortion (up to the 40th harmonic) are also measured by CPA with true RMS up to 400 Hz.The measured variables are available to the monitoring system connected through RS485, via Modbus/RTU communication.UCS (universal configuration software) installed onto a PC connected to CPA via RS485, allows to configure CPA (i.e. RS485 parameters) with ease and display measured variables in real time; configuration parameters are saved in both CPA's memory and UCS' database.UCS allows to create, edit and exchange configurations of both single CPA meters and complete networks of CPA units.CPA system architecture for dc systemCPA operating principles for dc systemsCPA is a power analyzer, measuring dc current in both directions with contactless Hall effect sensing and dc voltage with shunt based technology.Power and energy are also measured by CPA.The measured variables are available to the monitoring system connected through RS485, via Modbus/RTU communication.UCS (universal configuration software) installed onto a PC connected to CPA via RS485, allows to configure CPA (i.e. RS485 parameters) with ease and display measured variables in real time; configuration parameters are saved in both CPA's memory and UCS' database.UCS allows to create, edit and exchange configurations of both single CPA meters and complete networks of CPA units.CPA050DescriptionCPA050 is a power analyzer for dc or ac 1-phase applications.With a maximum current of 50 Aac/Adc and maximum voltage range of 800 Vac/1000 Vdc, it is the ideal solution for monitoring small photovoltaic installations, industrial processes, battery charging systems.Main features• True RMS ac (from 1 to 400 HZ) and dc monitoring• Current sensing by Hall effect; range: 50 Aac/Adc• Voltage range: 800 Vac/1000 Vdc• RS485 Modbus output; variables: A, V, W, var, VA, kW, HZ, PF, THD• 15 mm hole diameter• Din rail or panel, vertical or horizontal mountingMain functions• Compatible with VMU-C EM• Configurable by means of UCS (Universal Configuration Software) solution• Hall effect sensingApplicationsCPA power analyzers are the ideal solution for those applications which are beyond standard ac monitoring. Given their capability of working both at different frequency ranges, they match the needs of dc applications (battery charging, photovoltaic monitoring), of ac applications with high crest factor (UPS, variable frequency drives) and standard 1-phase ac installations.StructureFeaturesGeneralPower SupplyEnvironmentalCompatibility and conformityInputsMeasurementsAccuracyRS485InsulationConnection DiagramsFig. 1 ac input connection Fig. 2 dc input connectionFig. 3 Power supply Fig. 4 RS485Note for RS485:the serial output must be terminated on the last network device by means of a terminating unit according to Modbus standard; check grounding arrangements specification on the official Modbus documentation for proper grounding connections.Please check Multipoint System requirements at section 3.4 of the Modbus over serial line specification and implementation guide available at: /specs.phpReferencesFurther readingOrder codeCPA 050 1 L S1 XCARLO GAVAZZI compatible componentsCPA300DescriptionCPA300 is a power analyzer for dc or ac 1-phase applications.With a maximum current of 300 Aac/400 Adc and maximum installation voltage of 800 Vac/1000 Vdc, it is the ideal solution for monitoring medium/ large PV installations, industrial processes, battery charging systems.Main features• True RMS ac (from 1 to 400 HZ) and dc monitoring• Current sensing by Hall effect; range: 300 Aac/400 Adc • Voltage range: 800 Vac/1000 Vdc• RS485 Modbus output; variables: A, V, W, var, VA, kW, HZ, PF, THD• 33 mm hole diameter• Din rail or panel, vertical or horizontal mountingMain functions• Compatible with VMU-C EM• Configurable by means of UCS (Universal Configuration Software) solution• Hall effect sensingApplicationsCPA power analyzers are the ideal solution for those applications which are beyond standard ac monitoring. Given their capability of working both at different frequency ranges, they match the needs of dc applications (battery charging, photovoltaic monitoring), of ac applications with high crest factor (UPS, variable frequency drives) and standard 1-phase ac installations.StructureFeaturesGeneralPower SupplyEnvironmentalNote: R.H. < 90% non-condensing @ 40°C (104°F)Compatibility and conformityInputsMeasurementsAccuracyRS485InsulationConnection DiagramsFig. 5 ac input connection Fig. 6 dc input connectionFig. 7 Power supply Fig. 8 RS485Note for RS485:the serial output must be terminated on the last network device by means of a terminating unit according to Modbus standard; check grounding arrangements specification on the official Modbus documentation for proper grounding connections.Please check Multipoint System requirements at section 3.4 of the Modbus over serial line specification and implementation guide available at: /specs.phpReferencesFurther readingOrder codeCPA 300 1 L S1 XCARLO GAVAZZI compatible componentsCPA300VDescriptionCPA300V is a current transducer for dc or ac 1-phase applications.With a maximum current of 300 Aac/400 Adc and maximum installation voltage of 800 Vac/ 1500 Vdc, it is the ideal solution for monitoring medium/ large PV installations, industrial processes, battery charging systems.Main features• True RMS ac (from 1 to 400 HZ) and dc monitoring• Current sensing by Hall effect; range: 300 Aac/400 Adc • Maximum installation voltage: 800 Vac/1500 Vdc• RS485 Modbus output; variables: Amax, Amin, Ah• 33 mm hole diameter• Din rail or panel, vertical or horizontal mountingMain functions• Compatible with VMU-C EM• Configurable by means of UCS (Universal Configuration Software) solution• Hall effect sensingApplicationsCPA-300V is the ideal solution for those applications in which current only monitoring is required.Thanks to its high current range, its contactless Hall effect sensing and the maximum system voltage of 1500 Vdc, it fits perfectly the needs of medium to large size photovoltaic plant monitoring, where ease of installation and operation are mandatory requirements.StructureFeaturesGeneralPower SupplyEnvironmentalNote: R.H. < 90% non-condensing @ 40°C (104°F)Compatibility and conformityInputsOutputsMeasurementsAccuracyRS485InsulationConnection DiagramsFig. 9 ac input connection Fig. 10 dc input connection Fig. 11 Analogue outputFig. 12 Power supply Fig. 13 RS485Note for RS485:Please check Multipoint System requirements at section 3.4 of the Modbus over serial line specification and implementation guide available at: /specs.phpReferencesFurther readingOrder codeCPA 300 1 L S1 VCARLO GAVAZZI compatible componentsCOPYRIGHT ©2020Content subject to change. 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液晶弹性体
3. Actuators based on LCEs
3.1. Actuators based on thermally actuated LCEs
Fig 3. Micrometer-sized nematic LCE actuators consisting of a pillar array. (a) Experimental setup used to prepare the responsive pillars. (b) Top view (under an optical microscope) of the pillar pattern obtained by the imprint in the nematic liquid crystal elastomer. (Inset) Zoom on the structure (pillar diameter=20mm)[1]. [1 ]Buguin A, Li M H, Silberzan P, et al. Journal of the American Chemical Society, 2006, 128(4): 1088-1089.
4. Summary
1.Introduction
Smart materials:
There is a group of materials capable of responding to external stimuli with mechanical deformation.
Fig 1. The diferent kinds of actuator materials both in natural and synthetic systems
3. Actuators based on LCEs
LORD Corporation 产品说明书 - CHEMLOK 8560S-1
USA SAFETY DATA SHEET1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATIONProduct name: CHEMLOK 8560S-1Product Use/Class: Aqueous AdhesiveLORD Corporation111 LORD DriveCary, NC 27511-7923 USATelephone: 814 868-3180Non-Transportation Emergency: 814 763-2345 Chemtrec 24 Hr Transportation Emergency No.800 424-9300 (Outside Continental U.S. 703 527-3887)EFFECTIVE DATE: 12/20/20212. HAZARDS IDENTIFICATIONGHS CLASSIFICATION:Serious eye damage/eye irritation Category 2B Skin sensitization Category 1A Carcinogenicity Category 2Specific target organ systemic toxicity (single exposure) Category 2 blood system Specific target organ systemic toxicity (repeated exposure) Category 2 blood system Hazardous to the aquatic environment - acute hazard Category 2 Hazardous to the aquatic environment - chronic hazard Category 2GHS LABEL ELEMENTS:Symbol(s)Signal WordW ARNINGHazard StatementsCauses eye irritation.May cause an allergic skin reaction. Suspected of causing cancer.May cause damage to organs.(blood system)May cause damage to organs through prolonged or repeated exposure.(blood system) Toxic to aquatic life.Toxic to aquatic life with long lasting effects.Precautionary Statements PreventionObtain special instructions before use.Do not handle until all safety precautions have been read and understood. Wear protective gloves.Use personal protective equipment as required. Do not breathe dust/fume/gas/mist/vapors/spray. Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Contaminated work clothing should not be allowed out of the workplace. Avoid release to the environment.Product: CHEMLOK 8560S-1, Effective Date: 12/20/2021ResponseGet medical advice/attention if you feel unwell.Call a POISON CENTER or doctor/physician if exposed or you feel unwell.Specific treatment (see supplemental first aid instructions on this label).IF ON SKIN: Wash with plenty of soap and water.If skin irritation or rash occurs: Get medical advice/attention.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.If eye irritation persists: Get medical advice/attention.Wash contaminated clothing before reuse.Collect spillage.StorageStore locked up.Disposal:Dispose of contents/container in accordance with waste/disposal laws and regulations of your country or particular locality.Other Hazards:This product contains component(s) which have the following warnings; however based on the GHS classification criteria of your country or locale, the product mixture may be outside the respective category(s).Acute: May cause mild skin irritation. In elevated-temperature applications, product may release vapors that may produce cyanosis in the absence of sufficient ventilation or adequate respiratory protection. 4,4'-Diphenylmethane bis-maleimide is harmful by inhalation. Avoid breathing sanding dust from this product. May be harmful ifswallowed. Ingestion is not an expected route of entry in industrial or commercial uses.Chronic: Prolonged or repeated contact may result in dermatitis. The nitrogen substituted aromatic in this product gave positive results for mutagenicity in an Ames Assay study while two other mutagenicity studies proved negative.IARC has designated carbon black as Group 2B - inadequate evidence for carcinogenicity in humans, but sufficient evidence in experimental animals. In 2006 IARC reaffirmed its 1995 finding that there is "inadequate evidence"from human health studies to assess whether carbon black causes cancer in humans. Further, epidemiologicalevidence from well-conducted investigations has shown no causative link between carbon black exposure and the risk of malignant or non-malignant respiratory disease in humans. IARC has identified the proprietary curative in this product as an "animal suspected" carcinogen, Group 3, which downgrades a previous NCI report of it as an "animal positive" carcinogen.3. COMPOSITION/INFORMATION ON INGREDIENTSChemical Name CAS Number RangeNitrogen substituted aromatic PROPRIETARY15 - 20%Zinc compound PROPRIETARY 5 - 10%4,4'-Diphenylmethane bis-maleimide13676-54-5 1 - 5%Carbon black1333-86-4 1 - 5%Nonylphenol ethoxylate compound PROPRIETARY0.9 - 1%Curative PROPRIETARY0.1 - 0.9%Any "PROPRIETARY" component(s) in the above table is considered trade secret, thus the specific chemical and its exact concentration is being withheld.4. FIRST AID MEASURESFIRST AID - EYE CONTACT: Flush eyes immediately with large amount of water for at least 15 minutes holding eyelids open while flushing. Get prompt medical attention.FIRST AID - SKIN CONTACT: Flush contaminated skin with large amounts of water while removing contaminated clothing. Wash affected skin areas with soap and water. Get medical attention if symptoms occur.FIRST AID - INHALATION: Move person to fresh air. Restore and support continued breathing. If breathing is difficult, give oxygen. Get immediate medical attention.Product: CHEMLOK 8560S-1, Effective Date: 12/20/2021FIRST AID - INGESTION: If swallowed, do not induce vomiting. Call a physician or poison control center immediately for further instructions. Never give anything by mouth if victim is rapidly losing consciousness, unconscious or convulsing.5. FIRE-FIGHTING MEASURESSUITABLE EXTINGUISHING MEDIA: Carbon Dioxide, Dry Chemical, Foam, Water FogUNSUITABLE EXTINGUISHING MEDIA: Not determined for this product.SPECIFIC HAZARDS POSSIBLY ARISING FROM THE CHEMICAL: Keep containers tightly closed. Closed containers may rupture when exposed to extreme heat. Use water spray to keep fire exposed containers cool. WARNING: Due to the combustible nature of the dried film of this product and the potential for smoldering or fire, the accumulation and buildup of the dried film on spray booth walls and floors, spindles, fixtures and other surfaces should be avoided, and any buildup should be removed. Keep the dried film accumulations away from sparks, friction, impact, high heat (>235 F/>112 C) or other sources of ignition. These conditions could cause the dried film to ignite very readily and quickly, and the resulting smoldering or fire may be difficult to extinguish. During removal of accumulation/buildup of this product, take precautions to avoid heat, friction and impact during the cleaning process. Use paint stripper, brass brush, or plastic scraper for cleaning. In the event of smoldering or a fire involving the dried product, Cold Fire®** fire suppressing agent is preferred as the extinguishing medium. If Cold Fire® is not available, use water spray as the extinguishing medium. Take efforts to ensure that these agents reach the base of the smoldering or fire. Parker-LORD Corporation will not be responsible for personal injuries, property damage or any other damages arising from the accumulation (buildup, cleaning/removal or any related smoldering or fire) resulting from the use of this product. Refer to the Chemlok® Safe Handling Guide for additional information. **NOTE: Parker-LORD Corporation has determined Cold Fire® fire suppressing agent to be effective in extinguishing fires involving dried Chemlok® adhesives. Parker-LORD does not recommend any particular equipment or system for use in delivering or applying Cold Fire® products. Customer is responsible for determining that Cold Fire® products and any delivery equipment or system is appropriate and effective for customer's specific needs. During a fire, irritating and/or toxic gases and particulate may be generated by thermal decomposition or combustion. SPECIAL PROTECTIVE EQUIPMENT AND PRECAUTIONS FOR FIRE-FIGHTERS: Wear full firefighting protective clothing, including self-contained breathing apparatus (SCBA). If water is used, fog nozzles are preferable.6. ACCIDENTAL RELEASE MEASURESPERSONAL PRECAUTIONS, PROTECTIVE EQUIPMENT AND EMERGENCY PROCEDURES: Avoid breathing vapors. Avoid contact. Use appropriate respiratory protection for large spills or spills in confined area. See Section 5 for cautionary information on the dried residue of this product.ENVIRONMENTAL PRECAUTIONS: Do not contaminate bodies of water, waterways, or ditches, with chemical or used container.METHODS AND MATERIALS FOR CONTAINMENT AND CLEANUP: Notify appropriate authorities if necessary. Contain and remove with inert absorbent material. Avoid contact. Keep non-essential personnel away from spill area. Before attempting cleanup, refer to hazard caution information in other sections of this Safety Data Sheet.7. HANDLING AND STORAGEHANDLING: Keep closure tight and container upright to prevent leakage. Avoid skin and eye contact. Wash thoroughly after handling. Do not handle until all safety precautions have been read and understood. Empty containers should not be re-used. Use with adequate ventilation. See Section 5 for cautionary information on handling of the dried residue of this product. Avoid breathing sanding dust from this product.STORAGE: Store only in well-ventilated areas. Keep from freezing. Keep container closed when not in use. INCOMPATIBILITY: Strong acids, bases, and strong oxidizers.8. EXPOSURE CONTROLS/PERSONAL PROTECTIONCOMPONENT EXPOSURE LIMIT Chemical Name ACGIH TLV-TWA ACGIH TLV-STELOSHA PEL-TWAOSHA PEL-CEILINGSkinProduct: CHEMLOK 8560S-1, Effective Date: 12/20/2021Nitrogen substituted aromatic N.E.N.E.N.E. N.E. N.A. Zinc compound2 mg/m3 10 mg/m3 5 mg/m3 N.E. N.A. 4,4'-Diphenylmethane bis-maleimide N.E. N.E. N.E. N.E. N.A. Carbon black3 mg/m3 N.E. 3.5 mg/m3 N.E. N.A. Nonylphenol ethoxylate compound N.E. N.E. N.E. N.E. N.A. CurativeN.E.N.E.N.E.N.E.N.A.N.A. - Not Applicable, N.E. - Not Established, S - Skin DesignationEngineering controls: Sufficient ventilation in pattern and volume should be provided in order to maintain air contaminant levels below recommended exposure limits.PERSONAL PROTECTION MEASURES/EQUIPMENT:RESPIRATORY PROTECTION: Use a NIOSH approved chemical/mechanical filter respirator designed to remove a combination of particulates and organic vapor if occupational limits are exceeded. For emergencysituations, confined space use, or other conditions where exposure limits may be greatly exceeded, use an approved air-supplied respirator. For respirator use observe OSHA regulations (29CFR 1910.134) or use in accordance with applicable laws and regulations of your country or particular locality.SKIN PROTECTION: Use neoprene, nitrile, or rubber gloves to prevent skin contact.EYE PROTECTION: Use safety eyewear including safety glasses with side shields and chemical goggles where splashing may occur.OTHER PROTECTIVE EQUIPMENT: Remove and wash contaminated clothing before reuse.HYGIENIC PRACTICES: Wash hands before eating, smoking, or using toilet facility. Do not smoke in any chemical handling or storage area. Food or beverages should not be consumed anywhere this product is handled or stored. Wash thoroughly after handling.9. PHYSICAL AND CHEMICAL PROPERTIESTypical values, not to be used for specification purposes.ODOR:Odorless VAPOR PRESSURE:N.D.APPEARANCE: Green/BlackVAPOR DENSITY:Heavier than Air PHYSICAL STATE:Thixotropic liquid LOWER EXPLOSIVE LIMIT: Not Applicable FLASH POINT: ≥ 201 °F, 93 °C Setaflash Closed Cup UPPER EXPLOSIVE LIMIT:Not Applicable BOILING RANGE:100 °CEVAPORATION RATE: Slower than n-butyl-acetateAUTOIGNITION TEMPERATURE: N.D. DENSITY:1.2 g/cm3 (10.00 lb/gal) DECOMPOSITION TEMPERATURE:N.D. VISCOSITY, DYNAMIC: ≥50 mPa.s @ 25 °C ODOR THRESHOLD: N.D.VISCOSITY, KINEMATIC: ≥42 mm2/s @ 25 °C SOLUBILITY IN H2O:Water Dispersible VOLATILE BY WEIGHT: 56.93 % pH:6.0 VOLATILE BY VOLUME:66.72 % FREEZE POINT:N.D. VOC CALCULATED: 0.02 lb/gal , 3 g/l COEFFICIENT OF WATER/OILDISTRIBUTION:N.D.LEGEND: N.A. - Not Applicable, N.E. - Not Established, N.D. - Not Determined10. STABILITY AND REACTIVITYHAZARDOUS POLYMERIZATION: Hazardous polymerization will not occur under normal conditions.STABILITY: Product is stable under normal storage conditions.Product: CHEMLOK 8560S-1, Effective Date: 12/20/2021CONDITIONS TO AVOID: High temperatures.; For dried product issues, refer to Section 5 of the (M)SDS.; DO NOT ALLOW THIS MATERIAL TO DRY OUT. As a solid, p-benzoquinone dioxime is flammable, and it may explode if exposed to shock, friction or heat.INCOMPATIBILITY: Strong acids, bases, and strong oxidizers.HAZARDOUS DECOMPOSITION PRODUCTS: Decomposition due to high temperatures or a fire causes the formation of irritating and/or toxic gases, organic vapors or fumes., May contain CO, CO2, oxides of nitrogen, oxides of sulfur, halogenated by-products, Carbon dioxide, carbon monoxide, chlorine, hydrogen chloride, Phosgene, Metal oxides11. TOXICOLOGICAL INFORMATIONEXPOSURE PATH: Refer to section 2 of this SDS.SYMPTOMS: Refer to section 2 of this SDS.TOXICITY MEASURES:Chemical NameLD50/LC50 Nitrogen substituted aromatic Oral LD50: rat 1,100 mg/kgZinc compoundOral LD50: Rat > 5,000 mg/kg Dermal LD50: Rat > 2,000 mg/kgGHS LC50 (vapour): Acute toxicity point estimate 55 mg/l Inhalation LC50: Rat > 5,700 mg/m3 /4 h 4,4'-Diphenylmethane bis-maleimide Oral LD50: Rat > 5 g/kgDermal LD50: rat > 5,400 mg/kgGHS LC50 (dust and mist): rat 0.52 mg/l Inhalation ATE: 11 mg/l Carbon blackOral LD50: Rat > 15,400 mg/kg Dermal LD50: Rabbit > 3 g/kgGHS LC50 (vapour): Acute toxicity point estimate 55 mg/l : Nonylphenol ethoxylate compound N.D.CurativeOral LD50: Rat 464 mg/kgGHS LC50 (vapour): Acute toxicity point estimate 55 mg/l Inhalation LC50: Rat > 5 mg/l /4 hGerm cell mutagenicity: No classification proposedCarcinogenicity: Category 2 - Suspected of causing cancer. Components contributing to classification: Curative.Reproductive toxicity: No classification proposed12. ECOLOGICAL INFORMATIONECOTOXICITY:Chemical NameEcotoxicityNitrogen substituted aromatic N.D. Zinc compoundN.D.4,4'-Diphenylmethane bis-maleimide Fish: Oncorhynchus mykiss (rainbow trout) > 0.145 mg/l96 hCarbon blackN.D. Nonylphenol ethoxylate compound N.D.CurativeFish: Danio rerio (zebra fish) 24 mg/l96 h Static Invertebrates: Daphnia magna (Water flea) 3.5 mg/l48 h StaticPERSISTENCE AND DEGRADABILITY: Not determined for this product.BIOACCUMULATIVE: Not determined for this product.MOBILITY IN SOIL: Not determined for this product.OTHER ADVERSE EFFECTS: Not determined for this product.13. DISPOSAL CONSIDERATIONSDISPOSAL METHOD: Disposal should be done in accordance with Federal (40CFR Part 261), state and local environmental control regulations. If waste is determined to be hazardous, use licensed hazardous waste transporter and disposal facility. Waste streams, including the dried adhesive residue, resulting from the use of this product should be tested for RCRA characteristics, including ignitability, to determine any applicable waste classifications.14. TRANSPORT INFORMATIONUS DOT RoadProper Shipping Name: Environmentally hazardous substances, liquid, n.o.s.Hazard Class: 9SECONDARY HAZARD: NoneUN/NA Number: 3082Packing Group: IIIEmergency Response Guide Number: 171For US DOT non-bulk road shipments this material may be classified as NOT REGULATED. For the mostaccurate shipping information, refer to your transportation/compliance department regarding changes inpackage size, mode of shipment or other regulatory descriptors.IATA CargoPROPER SHIPPING NAME: Environmentally hazardous substance, liquid, n.o.s.Hazard Class: 9HAZARD CLASS: NoneUN NUMBER: 3082PACKING GROUP: IIIEMS: 9LIMDGPROPER SHIPPING NAME: Environmentally hazardous substance, liquid, n.o.s.Hazard Class: 9HAZARD CLASS: NoneUN NUMBER: 3082PACKING GROUP: IIIEMS: F-AThe listed transportation classification applies to non-bulk shipments. It does not address regulatory variations due to changes in package size, mode of shipment or other regulatory descriptors. For the most accurate shipping information, refer to your transportation/compliance department.15. REGULATORY INFORMATIONU.S. FEDERAL REGULATIONS: AS FOLLOWS:SARA SECTION 313This product contains the following substances subject to the reporting requirements of Section 313 of Title III of the Superfund Amendment and Reauthorization Act of 1986 and 40 CFR part 372.:Chemical Name CAS Number Weight % Less ThanZinc compound PROPRIETARY10.0%TOXIC SUBSTANCES CONTROL ACT:INVENTORY STATUSThe chemical substances in this product are on the active TSCA Section 8 Inventory or exempt.EXPORT NOTIFICATIONThis product contains the following chemical substances subject to the reporting requirements of TSCA 12(B) if exported from the United States:None16. OTHER INFORMATIONUnder HazCom 2012 it is optional to continue using the HMIS rating system. It is important to ensure employees have been trained to recognize the different numeric ratings associated with the HazCom 2012 and HMIS schemes.HMIS RATINGS - HEALTH: 2* FLAMMABILITY: 1 PHYSICAL HAZARD: 0* - Indicates a chronic hazard; see Section 2Revision: New GHS SDS FormatEffective Date: 12/20/2021DISCLAIMERThe information contained herein is, to the best of our knowledge and belief, accurate. However, since the conditions of handling and use are beyond our control, we make no guarantee of results, and assume no liability for damages incurred by use of this material. It is the responsibility of the user to comply with all applicable federal, state and local laws and regulations.。
Seaaonality and infestation pattern of epibiosis in the beach mysid Archaeomysis articulata
122Figure2.Map showing study area.123124125126(water temperature,salinity,dissolved oxygen,etc.). Primary productivity is apparently higher in boreal waters than in the southern Kuroshio region in general (e.g.Terazaki,1990).Moreover,the biomass of beach mysids is greater in the northern region(Hanamura, pers.observ.),where the epibiontic association thrives. High levels of infestation in the entire population of A. articulata(100%or close to this level)may be due to the epibiont re-attaching to the new substratum shortly after moulting occurs.The intensity of infestation differed between adult males and adult females,and the latter,usually ovi-gerous,were recognized to be a better substratum than males.A similar pattern has been found in epibiosis of some decapod crustaceans(Abelló&Macpherson, 1992;Maldonado&Urtiz,1992).The difference in intensity between males and females is explained by the difference in behaviour between the sexes,as well as the difference in intermoult duration.Hanamura& Nagasaki(1996)indicated that the infestation of cili-ates on Archaeomysis is most successful in the zone closest to the shoreline.In contrast,incidence in the infralittoral mysid Archaeomysis japonica Hanamura et al.,1996,remains at lower levels,even along the northern coasts.Takahashi&Kawaguchi(1997) demonstrated that in Archaeomysis kokuboi Ii,1964, the species most closely affiliated with A.articulata, mature males swim actively at night while brooding females remain in the near shore habitat all day.There-fore,it is likely that the ciliates benefit from brooding females spending more time resting in the intertidal zone.Comparison of epibiont prevalence among the size categories showed that the small-sized group(<5mm) was less infested than the larger groups(>5mm). Periodic moults in juveniles probably contribute to the reduced epibiont incidence.Similarly,the density of epibionts in stage1brooding females was consist-ently lower than the density of epibionts in advanced brooding females,resulting from an initial phase of re-colonisation on the new substratum after the copu-lation moult.Our results indicate that the prevalence and degree of infestation are,to some extent,related to body length and the time elapsed since moulting.Epibionts could attach to embryos in the female marsupium.However,infestation does not begin until the stage III embryo(about1.5mm or slightly larger in total length),since epibionts were observed only on embryos of this stage.The overall prevalence averaged 34.3%.In contrast,stage I and II embryos were totally devoid of the epibiont.A short intermoult period of the embryo alone is not sufficient to explain this res-ult,since the incubation period of spring embryos in Ishikari Bay is about1.5months(Hanamura,1999). There is no data on the moult period of embryos of A.articulata.Matsudaira et al.(1952)showed that A. kokuboi(as Gastrosaccus vulgaris)embryos remain for about2–7days at each instar stage at about11◦C. These facts suggest that early stage larvae may have a defensive ability against fouling by epibionts(see also Wahl,1989;Becker,1996).It has been reported that microbial fouling causes mortality of eggs and larvae of some decapod crustaceans(cf.Fisher&Wickham, 1976;Harper&Talbot,1984).Unfortunately,no at-tempt to evaluate this possibility could be carried out, so the effect of epibionts on last stage embryos of A. articulata deserves future investigation.It is possible that greater epibiont infestation causes some damage to the mysid population.A pre-liminary analysis to test the impact of epibionts on the population dynamics of A.articulata showed that greater epibiont infestation was not strongly correlated with mysid abundance.The results,therefore,imply that ciliate infestation did not influence the popula-tion dynamics of A.articulata,as has been suggested previously by Hanamura&Nagasaki(1996). AcknowledgementsI am indebted to Dr Olivie Decamp(formerly in our institution)for reading the manuscript.N.Ohtake and M.Iwabuchi helped with thefield work. ReferencesAbelló,P.&E.Macpherson,1992.Epibiosis and rhizocephalan infestation patterns in relation to the reproductive biology of Lithodes ferox(Filhol,1885)(Anomura:Lithodidae).J.crust.Biol.12:561–570.Abelló,P.,R.Villanueva&G.M.Gili,1990.Epibiosis in deep-sea crab populations as indicator of biological and behavioural characteristics of the host.J.mar.biol.Ass.U.K.70:687–695. Allen,Y.C.,B.T.De Stasio&C.W.Ramcharan,1993.Indi-vidual and population level consequences of an algal epibiont on Daphnia.Limonol.Oceanogr.38:592–601.Becker,K.,1996.Epibionts on carapaces of some malacostracans from the Gulf of Thailand.J.crust.Biol.16:92–104. Botton,M.L.&J.W.Ropes,1988.An indirect method for estimat-ing longevity of the horseshoe crab(Limulus polyphemus)based on epifaunal slipper shells(Crepidula fornicata).J.Shellfish Res.7:407–412.Chiavelli,D.A.,ls&S.T.Threlkeld,1993.Host pref-erence,seasonality and community interactions of zooplankton epibionts.Limonol.Oceanogr.38:574–583.127Couch,J.A.,1983.Diseases caused by Protozoa.In Provienzano,A.J.,Jr.(ed.),The Biology of Crustacea,vol.6,Pathobiology.Academic Press,New York:79–111.Evans,M.S.,L.M.Sicko-Goad&M.Omair,1979.Seasonal oc-currence of Tokophyra quadripartita(Suctoria)as epibionts on adult Limonocalanus macrurus(Copepoda:Calanoida)in south-eastern Lake Michigan.Trans.am.microsc.Soc.98:102–109. Evans,M.S.,D.W.Sell&A.M.Beeton,1981.Tokophyra quadri-partita and Tokophyra sp.(Suctoria)associations with crustacean zooplankton in the Great Lakes region.Trans.am.microsc.Soc.100:384–391.Fisher,W.S.&D.E.Wickham,1976.Mortalities and epibiotic fouling of eggs from wild populations of the dungeness crab, Cancer magister.Fish.Bull.74:201–207.Gili,J.-M.,P.Abelló&R.Villanueva,1993.Epibionts and in-termoult duration in the crab Bathynectes piperitus.Mar.Ecol.Prog.Ser.98:107–113.Green,J.,1974.Parasites and epibionts of Cladocera.Trans.zool.Soc.Lond.32:417–515.Hanamura,Y.,1997.Review of the taxonomy and biogeography of shallow-water mysids of the genus Archaeomysis(Crustacea: Mysidacea)in the North Pacific Ocean.J.nat.Hist.31:669–711. Hanamura,Y.,1999.Seasonal abundance and life cycle of Archae-omysis articulata(Crustacea:Mysidacea)on a sandy beach of western Hokkaido,Japan.J.nat.Hist.33:1811–1830. Hanamura,Y.&K.Nagasaki,1996.Occurrence of the sandy beach mysids Archaeomysis spp.(Mysidacea)infested by epibiontic peritrich ciliates(Protozoa).Crust.Res.25:25–33.Harper,R.E.&P.Talbot,1984.Analysis of the epibiotic bacteria of lobster(Homarus)eggs and their influence on the loss of eggs from the pleopods.Aquaculture36:9–26.Henebry,M.S.&B.T.Ridgeway,1979.Epizoic ciliated Protozoa of planktonic copepods and cladocerans and their possible use as indicators of organic water pollution.Trans.am.microsc.Soc.98:495–508.Hudson,D.A.&R.J.G.Lester,1994.Parasites and symbionts of wild mud crabs Scylla serrata(Forskal)of potential significance in aquaculture.Aquaculture120:183–199.Lindley,J.A.,1978.Continuous plankton records:the occurrence of apostome ciliates(Protozoa)on Euphausiacea in the North Atlantic Ocean and North Sea.Mar.Biol.46:131–136.Lópes,C.,E.Ochoa,R.Páez&S.Theis,1998.Epizoans on a tropical freshwater crustacean assemblage.Mar.Freshwat.Res.49:271–276.Maldonado,M.&M.J.Uriz,1992.Relationship between sponges and crabs:patterns of epibiosis on Inachus aguiarii(Decapoda: Majidae).Mar.Biol.113:281–286.Matsudaira, C.,T.Kariya&T.Tsuda,1952.The study on the biology of a mysid Gastrosaccus vulgaris Nakazawa.Tohoku J.agricul.Res.3:155–174.Mauchline,J.,1980.The biology of mysids and euphausiids.Adv.mar.Biol.18:1–676.Nagasawa,S.,1986.The peritrich ciliate Zoothamnium attached to the copepod Centropages abdominalis in Tokyo Bay waters.Bull.mar.Sci.38:553–558.Nicol,S.,1984.Ephelota sp.,a suctorian found on the euphausiid Meganyctiphanes norvegica.Can.J.Zool.62:744–746. Svavarsson,J.&B.Davídsdóttir,1994.Foraminiferan(Protozoa) epizoites on Arctic isopods(Crustacea)as indicators of isopod behaviour.Mar.Biol.118:239–246.Takahashi,K.&K.Kawaguchi,1997.Diel and tidal migrations of the sand-burrowing mysids,Archaeomysis kokuboi,A.japonica and Iiella ohshimai,in Otsuchi Bay,northern Japan.Mar.Ecol.Progr.Ser.148:95–107.Terazaki,M.,1990.Plankton and productivity around Japan.In Coastal Oceanography Research Committee,The Oceanograph-ical Society of Japan(ed.),Coastal Oceanography of Japanese Islands.Supplementary volume,Tokai University Press,Tokyo: 265–281.Turner,J.T.,M.T.Postek&S.B.Collard,1979.Infestation of the estuarine copepod Acartia tonsa with the ciliate Epistylis.Trans.am.microsc.Soc.98:136–138.Xu,Z.&C.W.Burns,1991.Effects of the epizoic ciliate,Epistylis daphniae,on growth,reproduction and mortality of Boeckella triarticulata(Thomson)(Copepoda:Calanoida).Hydrobiologia 209:183–189.Wahl,M.,1989.Marine epibiosis.I.Fouling and antifouling:some basic aspects.Mar.Ecol.Prog.Ser.58:175–189.Weisman,P.,D.J.Lonsdado&J.Yen,1993.The effect of peritrich ciliates on the production of Acartia hudsonica in Long Island Sound.Limnol.Oceanogr.38:613-622.Wiktor,K.&A.Krajewska-Soltys,1994.Occurrence of epizoic and parasitic protozoans on Calanoida in the southern Baltic.Bull.Sea Fish.Inst.2(132):13–25.Willey,R.L.,P.A.Cantrell&S.T.Threlkeld,1990.Epibi-otic euglenoidflagellates increase the susceptibility of some zooplankton tofish predation.Limnol.Oceanogr.35:952–959.。
AW00136801000_Getting_Started_with_pylon5_and_OpenCV
GetenCV
1
AW00136801000
Basler Application Note
1 Introduction
OpenCV is an open-source computer vision library that allows you to perform image processing on Basler Machine Vision cameras. This application note provides information on how to install and use OpenCV in combination with Basler’s pylon Camera Software Suite in Microsoft Visual Studio on Windows operating systems. OpenCV does not support Machine Vision standards such as USB3 Vision, GigE Vision, or IEEE 1394 (FireWire). Therefore, it is not recommended to grab images using OpenCV API functions. Instead, Basler recommends using the pylon Camera Software Suite SDK to grab images and convert them to OpenCV images. This document provides further information on the integration of OpenCV functions for image display, image saving, and video recording into your pylon source code.
Matrox Odyssey Xpro与IMPERX IPX-2M30H-L相关的Camera In
IMPERX IPX-2M30H-L June 8, 2009 Basics about thecameraCamera Descriptions▪ Effective resolution: 1920 ⨯ 1080 ⨯ 12-bit @ 32 fps.▪ Camera Link BASE interface (Dual tap).▪ Progressive scan.▪ Internal sync.▪ External or internal exposure control.▪ 40 MHz pixel clock rate.Mode of operations as per Matrox Imaging (in parentheses as per camera manufacturer)Interface Mode▪ Continuous▪ Pseudo-continuous (CC Expose Control = Computer) ▪ Asynchronous reset (CC Expose Control = Computer)Basics about theinterface modesCamera Interface BriefsMode 1: Continuous▪ 1920 ⨯ 1080 ⨯ 12-bit @ 32 fps.▪ Camera Link BASE interface (Dual tap).▪ Progressive scan.▪ Matrox Odyssey Xpro receiving LVAL, FVAL, PCLK and video fromcamera.▪ DCF used: 2M30HL_1920x1080_12bitCon.DCFMode 2: Pseudo-Continuous▪ 1920 ⨯ 1080 ⨯ 12-bit.▪ Camera Link BASE interface (Dual tap).▪ Progressive scan.▪ Matrox Odyssey Xpro sending TIMER1 OUT (CC1) signal to camera toinitiate and control the exposure.▪ Matrox Odyssey Xpro receiving LVAL, FVAL, PCLK and video fromcamera.▪ DCF used: 2M30HL_1920x1080_12bitPcon.DCFIMPERX IPX-2M30H-L June 8, 2009Basics about theinterface modes Camera Interface Briefs (cont.)Mode 2: Pseudo-ContinuousMode 3: Asynchronous reset ▪ 1920 ⨯ 1080 ⨯ 12-bit.▪ Camera Link BASE interface (Dual tap).▪ Progressive scan.▪ Matrox Odyssey Xpro receiving external trigger signal.▪ Matrox Odyssey Xpro sending TIMER1 OUT (CC1) signal to camera toinitiate and control the exposure.▪ Matrox Odyssey Xpro receiving LVAL, FVAL, PCLK and video fromcamera.▪ DCF used: 2M30HL_1920x1080_12bitAsync.DCFSpecifics aboutthe interface modes Camera Interface DetailsMode 1: Continuous▪ Frame Rate: Matrox Odyssey Xpro receives the continuous video fromthe camera at 32 frames per second.▪ Exposure time: Exposure time is set using the Imperx CameraConfiguration Utility. Refer to the camera manual for commandsdescription and usage.Continued…IMPERX IPX-2M30H-L June 8, 2009 Specifics aboutCamera Interface Details (cont.)the interface modesMode 1: Continuous▪Camera Configuration: The camera mode is set as follows using theImperx Camera Configuration Utility. Refer to the camera manual forcommands description and usage.Mode SettingBit Depth 12 bitsOutput Mode Dual TapsTrigger OFFMode 2: Pseudo-Continuous▪Frame rate: The frame rate is determined by the frequency of theTIMER1 OUT (CC1) signal.▪Exposure time: The exposure is determined by the active duration ofTIMER1 OUT (CC1), which can be modified in the DCF using MatroxIntellicam, ONL imCamControl() or imDigControl() function, or with theMIL MdigControl() function. Consult the respective manual for moreinformation.▪Camera Configuration: The camera mode is set as follows using theImperx Camera Configuration Utility. Refer to the camera manual forcommands description and usage.Mode SettingBit Depth 12 bitsOutput Mode Dual TapsTrigger CCMode StandardCC Expose Control ComputerMode 3: Asynchronous Reset▪Frame rate: The frame rate is determined by the frequency of theexternal trigger signal.▪Exposure time: Same as in Mode 2: Pseudo-continuous.▪Camera Configuration: Same as in Mode 2: Pseudo-continuous.Cabling details for theCabling Requirementsinterface modesModes 1 and 2: Continuous, Pseudo-continuous▪Cable and Connection: Standard Camera Link cable.Matrox Odyssey XproCamera Interface Application NoteIMPERX IPX-2M30H-L June 8, 2009 Cabling details for theCabling Requirements (cont.)interface modesMode 3: Asynchronous reset▪Cable and Connection: Standard Camera Link.▪External trigger: External trigger should be connected to the OPTOTRIG input of the 9-pin connector (pins 7 and 2) on the Expanded I/Oadapter bracket.EXPANDED I/O BRACKET(9-pin connector) External Trigger SourceOPTOTRIG + 07 ←LINE SIGNAL --OPTOTRIG - 02 ←LINE GROUND --The DCFs mentioned in this application note are also attached (embedded) to this PDF file – use the Adobe Reader’s View File Attachment to access the DCF files. The information furnished by Matrox Electronics System, Ltd. is believed to be accurate and reliable. Please verify all interface connections with camera documentation or manual. Contact your local sales representative or Matrox Sales office or Matrox Imaging Applications at 514-822-6061 for assistance. © Matrox Electronic Systems Ltd, 2009-2011.Matrox Electronic Systems Ltd.1055 St. Regis Blvd.Dorval, Quebec H9P 2T4CanadaTel: (514) 685-2630Fax: (514) 822-6273。
各类质粒载体图谱
pGADT7
Vector Information
as a fusion to a hemagglutinin (HA) epitope tag. HA-tagged proteins can be identified with antibodies raised to this common epitope, eliminating the need to generate specific antibodies to new proteins. The T7 promoter is used for in vitro transcription and translation of the epitope tagged fusion protein and also provides a binding site for sequencing using the T7 Sequencing Primer. Note that the AD is not expressed during the in vitro transcription and translation reactions. The Nco I and Pst I sites may be used to shuttle inserts from pGADT7 into pGBKT7, the MATCHMAKER Two-Hybrid System 3 DNA-BD Vector. The MCS in pGADT7 is compatible with those in pMyc-CMV and pHA-CMV, CLONTECH's epitope tagged mammalian expression vector set (#K6003-1). As a result, the target gene can be shuttled into these vectors in order to confirm protein interactions in vivo. Location of features: • Full-length S. cerevisiae ADH1 promoter (PADH1): 7–1479 • GAL4 AD polypeptide with SV40 Nuclear Localization Signal (NLS) NLS: 1501–1557 GAL4 amino acids 768–881: 1561–1899 • T7 RNA polymerase promoter: 1905–1927 • HA epitope tag: 1942–1968 • Multiple Cloning Sites: 1969–2041 • Transcription termination signal Fragment carrying the S. cerevisiae ADH1 terminator (TADH1): 2280–2605 • LEU2 coding sequences: 3814–2723 • pUC plasmid replication origin: 4581–5418 • Ampicillin resistance gene: 6432–5575 • Yeast 2 µ replication origin: 6998–7988 Location of primers: • T7 Sequencing Primer: 1905–1925 • 3' AD Sequencing Primer: 2102–2083 • MATCHMAKER 5' AD LD-Insert Screening Amplimer (#9103-1): 1858–1889 • MATCHMAKER 3' AD LD-Insert Screening Amplimer (#9103-1): 2078–2046 Propagation in E. coli: • Suitable host strains: DH5α, DH10 & other general purpose strains • Selectable marker: plasmid confers resistance to ampicillin (100 µg/ml) to E. coli hosts • E. coli replication origin: pUC • Copy number: ~500 • Plasmid incompatibility group: pMB1/Col E1 Propagation in S. cerevisiae: • Suitable host strains: Y187(α), Y190(a), SFY526(a), CG1945(a), HF7c(a), or AH109(a) • Selectable marker: LEU2 • S. cerevisiae origin: 2 µ Reference:
罗克威自动化有限公司 GuardLogix 5580 控制器和 ControlLogix 安全 I
Confirmation of Product Type ApprovalCompany Name:ROCKWELL AUTOMATION,INC.Address:1ALLEN-BRADLEY DR.OH44124United StatesProduct:Computers and Programmable Logic Controllers(PLCS)Model(s):GuardLogix5580Controllers and ControlLogix Safety I/O ModulesCertificate Type Certificate Number Issue Date Expiry DateProduct Design Assessment(PDA) Manufacturing Assessment(MA) Product Quality Assurance(PQA)20-2006633-PDA17-C3320064NA23-JUN-202024-APR-2017NA22-JUN-202523-APR-2022NATier3Intended ServiceMarine and Offshore Applications-Programmable Logic Controllers and I/O Modules for Monitoring and Control Systems(acceptable for vessels with DPS,ACC&ACCU Notation vessels)DescriptionComponents comprise of programmable logic controllers and I/O Modules.The GuardLogix controller is made up of a primary controller and a safety partner,for SIL3applications, that function together in a1oo2architecture.Controllers Catalog numbers:1756-L81ES,1756-L82ES,1756-L83ES,1756-L84ESSafety Partner Catalog number:1756-L8SPControlLogix Safety I/O Modules:1756-IB16S:This module is a16-point sinking safety input module.1756-OBV8S:This module is an8-point,non-isolated digital safety output module that provides sourcing outputs and bipolar type outputs.Products listed above may also include suffix"K"which denotes conformal coating.Firmware Rev.1.XXXRatingsGuardlLogix5580Controllers:Power Supply:1.2A@5.1VDC,5mA@1.2VDCOperating Temperature:0°C to60°C(for Series C Chassis)Note:If operating above+55°C(+131°F),modules greater than6.2W shall not be installed in slots adjacent to the controller.ControlLogix Safety I/O Modules:Power Supply:1756-IB16S-Back Plane:280mA,5.1VDC;Field:18-32VDC,1.8A SELV;In:10-32VDC,2.5mA;To:18-32VDC,200mA1756-OBV8S-Back Plane:280mA,5.1VDC;Field:18-32VDC,1.8A SELV;Out:18-32V DC,1A; Operating Temperature:0°C to60°CService RestrictionsA.Where the product is used in a Category II or III Computer Based Systems as described in Section4-9-3/Table1of the Marine Vessel Rules,the complete assembly unit or subassembly unit is to be tested at the manufacturer’s shop in the presence of the Surveyor to verify the tests in4-9-9/15.7Table 2.Otherwise,unit certification is not required.B.If the manufacturer or purchaser request an ABS Certificate for compliance with a specification or standard,the specification or standard,including inspection standards and tolerances,must be clearly defined.C.Tests and approvals are for hardware only.D.Each particular application is to be specifically approved.E.An external enclosure of suitable ingress protection rating is to provided in accordanace with ABS MVR4-8-3/Table2.F.The products have not been tested for installation in weather exposed areas(Salt mist test as per ABS Marine Vessels Rules4-9-9/Table1:Test no.10has not been conducted).G.The subject controllers and I/O modules are powered by a dedicated and regulated power supply unit (not in the scope of this certifcate)via a proprietary backplane.The power supply variation test as per ABS Marine Vessels Rules4-9-9/Table1,Test no.1has not been conducted on the subject products. This test is to be witnessed by the attending surveyor as per Service restriction"A".CommentsA.The Manufacturer has provided a declaration about the control of,or the lack of Asbestos in this product.B.Duplicate PDA resides with Rockwell Automation-Twinsburg,OHC.The Conducted Low Frequency test per ABS Marine Vessels Rules4-9-9/Table1:Test no.13has not been performed on the subject controllers and I/O modules based on IEC61131-2standard,as this test is applicable to power ports.D.The Conducted Emission test per ABS Marine Vessels Rules4-9-9/Table1:Test no.18,has not been performed on the subject I/O modules as these modules donot have user accessible ports and power ports.Notes,Drawings and DocumentationRockwell Automation Publication1756-IN048B-EN-P-Feb2018,Installation Manual-GuardLogix5580 Controllers,Pgs:10;Rockwell Automation Publication1756-IN079B-EN-P-Oct2019,Installation Manual-1756ControlLogix 16-point Sinking Safety Input Module,Pgs:12;Rockwell Automation Publication1756-IN081B-EN-P-Oct2019,Installation Manual-1756ControlLogix 8-point Safety Bipolar/Sourcing Output Module,Pgs:12;Rockwell Automation Publication1756-UM543J-EN-P-Oct2019,User Manual-ControlLogix5580and GuardLogix5580Controllers Bulletin1756,Pgs:278;Rockwell Automation Publication1756-UM013B-EN-P-Oct2019,User Manual-1756ControlLogix Digital Safety I/O Modules,Pgs:136;Rockwell Automation Test Report no.951047,Technical Report-EMC Evaluation,date:26May2020, Pgs:29;Rockwell Automation Test Report no.MAY18-12M,rev.0,Technical Report-Environmental Test1756-IB16S,date:10-4-2018,Pgs:10;Rockwell Automation Test Report no.MAY18-13M,rev.0,Technical Report-Environmental Test1756-OBV8S/A,date:7Jan2019,Pgs:10;Rockwell Automation Test Report no.4009921,Technical Report-Environmental Test1756-OBV8S/A, date:10Oct2019,Pgs:73;Rockwell Automation Test Report no.74094,Technical Report-Environmental Test1756-L8zS,date:14 Oct2019,Pgs:115;Rockwell Automation Test Report no.902381,Technical Report-Environmental Test1756-IB16S,date:3 Dec2019,Pgs:83;Report No.F2LQ10272-02E,F2Labs EMC Test Report for GuardLogix5580Safety Controller,Issue date:17Nov2017,Pgs:55;Report No.F2P21798E-02E,F2Labs EMC Test Report for ControlLogix Safety I/O module,Issue date: 13Sep2019,Pgs:22;Report No.F2P21798E-01E,F2Labs EMC Test Report for ControlLogix Controller,Issue date:13Sep 2019,Pgs:32;Report No.F2P21798E-02E,F2Labs EMC Test Report for ControlLogix Controller,Issue date:13Sep 2019,Pgs:45;Ref.Email Correspondence regarding Multiple Unit Testing,Pgs:5;Ref.Response to comments in ABS Letter dated18Feb2020,Ref.Task no.T1951381,Pgs:4;Dwg.10001621409,ver.02,1756-L8zE5Schematic Drawing,pgs:18;Doc.1756Construction Overview,Pgs:6;Dwg.10001621417,ver.04,PCB ASM,1756-L8z E5PCB Assembly Drawing,Pgs:4;Dwg.10002950966,ver.04,PCB-SCH,1756-OBV8SI/O BRD E4.0Schematic Drawing,Pgs:27;Dwg.10002950988,ver.03,PCB ASM,1756-OBV8SI/O BRD3.0PCB Assembly Drawing,Pgs:2;Dwg.10003071310,ver.05,PCB SCH,1756-OBV8S MBE2Schematic Drawing,Pgs:42;Dwg.10003071319,ver.02,PCB ASM,1756-OBV8S MBE2PCB Assembly Drawing,Pgs:2;Dwg.10003323584,Assembly Readiness Checklist Safety IO,Pgs:2;Dwg.10003632086,Assembly Readiness Checklist L8zS,Pgs:2;Dwg.10003938061,1756-IB16S Main Board E1Schematic Drawing,ver.01,Pgs:47;Dwg.10003938063,PCB Documentation1756-IB16S Main Board E1PCB Assembly Drawing,ver.01, Pgs:2;Dwg.10003938069,ver.01,1756-IB16S I/O Board E1Schematic Drawing,Pgs:38;Dwg.10003938072,ver.01,PCB Documentation1756-IB16S E1I/O PCB Assembly Drawing,Pgs:1;Marine Test Plan1756-L84ES and L8SP,Pgs:1;GuardLogix Marine Test Plan20200204,Pgs:1;Doc.1756-L8z Master list20200204,Master list,pgs:1;A2LA Cert no.0793.02,F2Labs Scope of Accreditation to ISO/IEC17025:2017,Rev.date:01-02-2020, Pgs:5;UL File E65584,Letter Report for12mm Flame testing of Type1756case material,date:3Feb2020, Pgs:1;UL File E65584,Laboratory Data Package,Pgs:13;NVLAP Certificate of Accreditation to ISO/IEC17025:2017,Rockwell Automation-Mayfield Heights, Pgs:1;NVLAP Scope of Accreditation to ISO/IEC17025:2017,Rockwell Automation EMC TestLaboratory-Mayfield Heights,Pgs:4;IECEE Certificate of Acceptance to UL Northbrook,date of issue:2019-01-21,Pgs:2;Term of ValidityThis Product Design Assessment(PDA)Certificate remains valid until22/Jun/2025or until the Rules and/or Standards used in the assessment are revised or until there is a design modification warranting design reassessment(whichever occurs first).Acceptance of product is limited to the"Intended Service"details prescribed in the certificate and as per applicable Rules and Standards.This Certificate is valid for installation of the listed product on ABS units which exist or are under contract for construction on or previous to the effective date of the ABS Rules and standards applied at the time of PDA e of the Product for non-ABS units is subject to agreement between the manufacturer and intended client.ABS RulesRules for Conditions of Classification,Part1-2020Marine Vessels1-1-4/7.7,1-1-A3,1-1-A4,which covers the following:Marine Vessel Rules2020:4-9-9/3,4-9-9/15.7(Table1,Table2)International StandardsNAEU-MED StandardsNANational StandardsNAGovernment StandardsNAOther StandardsEU RO Mutual Recognition Technical Requirements for Computers and Programmable logic Controllers (PLCS)Version0.6,May2020.Corporate ABS ProgramsAmerican Bureau of ShippingPrint Date and Time:15-Sep-20201:40 ABS has used due diligence in the preparation of this certificate,and it represents the information on the product in the ABS Records as of the date and time the certificate is printed.If the Rules and/or standards used in the PDA evaluation are revised or if there is a design modification(whichever occurs first), a PDA revalidation may be necessary.The continued validity of the MA is dependent on completion of satisfactory audits as required by the ABS Rules.The validity of both PDA and MA entitles the product to receive a Confirmation of Product Type Approval.Acceptance of product is limited to the“Intended Service”details prescribed in the certificate and as per applicable Rules and Standards.This Certificate is valid for installation of the listed product on ABS units which exist or are under contract for construction on or prior to the effective date of the ABS Rules and standards applied at the time of PDA issuance.ABS makes no representations regarding Type Approval of the Product for use on vessels,MODUs or facilities built after the date of the ABS Rules used for this evaluation.Type Approval requires Drawing Assessment,Prototype Testing and assessment of the manufacturer's quality assurance and quality control arrangements.The manufacturer is responsible to maintain compliance with all specifications applicable to the product design assessment.Unless specifically indicated in the description of the product,certification under type approval does not waive requirements for witnessed inspection or additional survey for product use on a vessel,MODU or facility intended to be ABS classed or that is presently in class with ABS.Due to wide variety of specifications used in the products ABS has evaluated for Type Approval,it is part of our contract that; whether the standard is an ABS Rule or a non-ABS Rule,the Client has full responsibility for continued compliance with the standard.Questions regarding the validity of ABS Rules or the need for supplemental testing or inspection of such products should,in all cases,be addressed to ABS.。
DRAFT NIST Special Publication 800-38B DRAFT Recommendation for Block Cipher Modes of Opera
NIST Special Publication 800-38BDRAFTRecommendation for Block Cipher Modes of Operation: The RMAC Authentication ModeMethods and TechniquesMorris DworkinNovember 4, 2002AbstractThis Recommendation defines an authentication mode of operation, called RMAC, for a symmetric key block cipher algorithm. RMAC can provide cryptographic protection of sensitive, but unclassified, computer data. In particular, RMAC can provide assurance of the authenticity and, therefore, of the integrity of the data.KEY WORDS: Authentication; block cipher; cryptography; encryption; Federal Information Processing Standard; information security; integrity; mode of operation.Table of Contents1PURPOSE (5)2AUTHORITY (5)3INTRODUCTION (5)4DEFINITIONS, ABBREVIATIONS, AND SYMBOLS (6)4.1D EFINITIONS AND A BBREVIATIONS (6)4.2S YMBOLS (7)4.2.1Variables (7)4.2.2Operations and Functions (8)5PRELIMINARIES (9)5.1T HE U NDERLYING B LOCK C IPHER A LGORITHM (9)5.2E LEMENTS OF RMAC (9)5.3E XAMPLES OF O PERATIONS AND F UNCTIONS (10)6RMAC SPECIFICATION (10)6.1M ESSAGE F ORMATTING (10)6.2P ARAMETER S ETS (10)6.3MAC G ENERATION (11)6.4T AG G ENERATION AND V ERIFICATION (12)APPENDIX A: SECURITY CONSIDERATIONS (13)A.1E XHAUSTIVE K EY S EARCH (13)A.2G ENERAL F ORGERY (13)A.3E XTENSION F ORGERY B ASED ON A C OLLISION (13)A.4S UMMARY OF S ECURITY P ROPERTIES OF P ARAMETER S ETS (14)APPENDIX B: THE GENERATION OF RMAC PARAMETERS (15)B.1 D ERIVATION OF RMAC KEYS FROM A M ASTER K EY (15)B.2 S ALT G ENERATION (15)APPENDIX C: EXAMPLE VECTORS FOR THE MAC GENERATION FUNCTION (16)C.1RMAC-AES128 E XAMPLE V ECTORS (16)C.1.1RMAC-AES128-I (16)C.1.2RMAC-AES128-II (17)C.1.3RMAC-AES128-III (18)C.1.4RMAC-AES128-IV (19)C.1.5RMAC-AES128-V (20)C.2RMAC-AES192 E XAMPLE V ECTORS (21)C.2.1RMAC-AES192-I (21)C.2.2RMAC-AES192-II (22)C.2.3RMAC-AES192-III (23)C.2.4RMAC-AES192-IV (24)C.2.5RMAC-AES192-V (26)C.3RMAC-AES256 E XAMPLE V ECTORS (27)C.3.1RMAC-AES256-I (27)C.3.2RMAC-AES256-II (28)C.3.3RMAC-AES256-III (29)C.3.4RMAC-AES256-IV (30)C.3.5RMAC-AES256-V (32)C.4RMAC-TDES112 E XAMPLE V ECTORS (33)C.5RMAC-TDES168 E XAMPLE V ECTORS (33)APPENDIX D: REFERENCES (34)Table of FiguresFigure 1: The RMAC MAC Generation Function (12)1 PurposeThis publication is the second part in a series of Recommendations regarding modes of operation of symmetric key block cipher algorithms.2 AuthorityThis document has been developed by the National Institute of Standards and Technology (NIST) in furtherance of its statutory responsibilities under the Computer Security Act of 1987 (Public Law 100-235) and the Information Technology Management Reform Act of 1996, specifically 15 U.S.C. 278 g-3(a)(5). This is not a guideline within the meaning of 15 U.S.C. 278 g-3 (a)(5).This Recommendation is neither a standard nor a guideline, and as such, is neither mandatory nor binding on federal agencies. Federal agencies and nongovernment organizations may use this Recommendation on a voluntary basis. It is not subject to copyright.Nothing in this Recommendation should be taken to contradict standards and guidelines that have been made mandatory and binding upon federal agencies by the Secretary of Commerce under statutory authority. Nor should this Recommendation be interpreted as altering or superseding the existing authorities of the Secretary of Commerce, the Director of the Office of Management and Budget, or any other federal official.Conformance testing for implementations of the modes of operation that are specified in this Recommendation will be conducted within the framework of the Cryptographic Module Validation Program (CMVP), a joint effort of NIST and the Communications Security Establishment of the Government of Canada. An implementation of a mode of operation must adhere to the requirements in this Recommendation in order to be validated under the CMVP. The requirements of this Recommendation are indicated by the word “shall.”3 IntroductionThis Recommendation specifies an algorithm, RMAC [1], that can provide assurance of data origin authentication and, hence, assurance of data integrity. In particular, RMAC is an algorithm for generating a message authentication code (MAC) from the data to be authenticated and from an associated value called the salt, using a block cipher and two secret keys that the parties to the authentication of the data establish beforehand. One party generates the MAC and provides the MAC and the associated salt as the authentication tag; subsequently, any party with access to the secret keys may verify whether the received MAC was generated from the received data and the received salt. Successful verification of the MAC provides assurance of the authenticity of the data, i.e., that it originated from a source with access to the secret keys. Consequently, successful verification of the MAC also provides assurance of the integrity of the data, i.e., that it was not altered after the generation of the MAC.A MAC is sometimes called a cryptographic checksum, because it is generated from a keyed cryptographic algorithm in order to provide stronger assurance of data integrity than an ordinary checksum. The verification of an ordinary checksum or an error detecting code is designed to reveal only accidental modifications of the data, while the verification of a MAC is designed to reveal intentional, unauthorized modifications of the data, as well as accidental modifications. Because RMAC is constructed from a block cipher algorithm, RMAC can be considered a mode of operation of the block cipher algorithm. The block cipher algorithm shall be approved, i.e., specified or adopted in a Federal Information Processing Standard (FIPS) or a NIST Recommendation; for example, FIPS Pub. 197 [2] specifies the AES algorithm, and FIPS Pub. 46-3 [3] adopts the Triple DES algorithm.FIPS Pub. 198 [4] specifies a different MAC algorithm, called HMAC, that is also appropriate for the protection of sensitive data. Because HMAC is constructed from a hash function rather than a block cipher algorithm, RMAC may be preferable for application environments in which an approved block cipher is more convenient to implement than an approved hash function.4 Definitions, Abbreviations, and Symbols4.1 Definitions and AbbreviationsApproved FIPS approved or NIST recommended: an algorithm or technique thatis either 1) specified in a FIPS or NIST Recommendation, or 2) adoptedin a FIPS or NIST Recommendation.Authenticity The property that data indeed originated from its purported source. Authentication Mode A block cipher mode of operation that can provide assurance of theauthenticity and, therefore, the integrity of data.Authentication Tag (Tag) A pair of bit strings associated to data to provide assurance of its authenticity: the salt and the message authentication code that is derived from the data and the salt.Bit A binary digit: 0 or 1.Bit String An ordered sequence of 0s and 1s.Block A bit string whose bit length is the block size of the block cipheralgorithm.Block Cipher See forward cipher function.Block Cipher Algorithm A family of functions and their inverses that is parameterized by cryptographic keys; the functions map bit strings of a fixed length to bit strings of the same length.Block Size The number of bits in an input (or output) block of the block cipher. Cryptographic Key A parameter used in the block cipher algorithm that determines theforward cipher function.Data Integrity The property that data has not been altered by an unauthorized entity. Exclusive-OR The bitwise addition, modulo 2, of two bit strings of equal length. FIPS FederalInformationProcessing Standard.Forward Cipher Function One of the two functions of the block cipher algorithm that is determined by the choice of a cryptographic key.Initialization Vector(IV)A data block that some modes of operation require as an initial input.Message Authentication Code (MAC) A cryptographic checksum on data that is designed to reveal both accidental errors and intentional modifications of the data.Mode of Operation (Mode) An algorithm for the cryptographic transformation of data that features a symmetric key block cipher algorithm.Most Significant Bit(s) The left-most bit(s) of a bit string.Nonce A value that is used only once within a specified context.RMAC The name of the authentication mode that is specified in thisRecommendation.Salt A parameter of an algorithm whose role is to randomize the value ofanother parameter.4.2 Symbols4.2.1 Variablesb The block size, in bits.k The key length for the block cipher.m The bit length of the RMAC MAC.n The number of data blocks in the padded message.r The bit length of the salt.CNST j The j th fixed, i.e., constant, block.K A block cipher key.K1 The first RMAC key.K2 The second RMAC key.K3 A key that is derived from the second RMAC key and the salt.M The message.Mlen The bit length of the message.M j The j th block in the partition of the padded message.j th output block.O j ThePAD The padding that is appended to the message.salt.R The4.2.2 Operations and Functions0s The bit string consisting of s ‘0’ bits.X || Y The concatenation of two bit strings X and Y.X ⊕Y The bitwise exclusive-OR of two bit strings X and Y of the same length.CIPH K(X) The forward cipher function of the block cipher algorithm under the key K applied to the data block X.MSB s(X) The bit string consisting of the s most significant bits of the bit string X.RMAC(R,M) The RMAC message authentication code for message M with salt R.5 Preliminaries5.1 The Underlying Block Cipher AlgorithmThe RMAC algorithm specified in this Recommendation depends on the choice of an underlying symmetric key block cipher algorithm; the RMAC algorithm is thus a mode of operation (mode, for short) of the symmetric key block cipher. The underlying block cipher algorithm must be approved, and two secret, random keys for the block cipher algorithm shall be established. The keys regulate the functioning of the block cipher algorithm and, thus, by extension, the functioning of the mode. The specifications of the block cipher algorithm and the mode are public, so the security of the mode depends, at a minimum, on the secrecy of the keys.For any given key, the underlying block cipher algorithm of the mode consists of two processes that are inverses of each other. As part of the choice of the block cipher algorithm, one of the two processes of the block cipher algorithm is designated as the forward cipher function. The inverse of this process is called the inverse cipher function. Because the RMAC mode does not require the inverse cipher function, the forward cipher function in this Part of the Recommendation is simply called the block cipher.5.2 Elements of RMACThe block cipher keys that are required for the RMAC mode are bit strings, denoted K1and K2, whose bit length, denoted k, depends on the choice of the block cipher algorithm. The keys shall be random or pseudorandom, distinct from keys that are used for other purposes, and secret. The two keys shall each be established by an approved key establishment method, or the keys shall be derived from a single key K, which is established by an approved key establishment method.A method for deriving K1and K2 from a single, master key K is given in Appendix B.1.The block cipher is a function on bit strings of a fixed bit length. The fixed bit length of the bit strings is called the block size and is denoted b; any bit string whose bit length is b is called a (data) block. Under a key K, the block cipher function is denoted CIPH K.For the AES algorithm, b=128 and k=128, 192, or 256; for Triple DES, b=64 and k=112 or 168. The data to be authenticated is one input to the RMAC MAC generation function; the data in this context is called the message, denoted M.Another input to the MAC generation function is a parameter associated with the message called the salt, denoted R. The role of the salt in the MAC generation function is to randomize (i.e., “flavor”) the second key, K2. The bit length of the salt, denoted r, is determined by the choice of a parameter set that is specified in Section 6.2. The use of the salt is optional in the sense that a parameter set may be chosen in which r=0. When r1234567487569 4 9 4 7 7 5 45674 54 6 4 ensure that the expected probability of repeating the salt for different messages is negligible. The generation of the salt is discussed further in Appendix B.2.The RMAC MAC generation function is denoted RMAC, so that the output of the function, the MAC, is denoted RMAC(R,M). The bit length of the MAC, denoted m, is determined by thechoice of a parameter set that is specified in Section 6.2. The authentication tag to the message is the ordered pair (R, RMAC(R,M)); thus, the tag consists of one part, the salt, that may be independent of the message and a second part, the MAC, that depends on both the salt and the message. The total number of bits in the tag is r+m.5.3 Examples of Operations and FunctionsFor a nonnegative integer s, the bit string consisting of s ‘0’ bits is denoted 0s.The concatenation operation on bit strings is denoted ||; for example, 001 || 10111 = 00110111.Given bit strings of equal length, the exclusive-OR operation, denoted ⊕, specifies the addition, modulo 2, of the bits in each bit position, i.e., without carries. Thus, 10011 ⊕ 10101= 00110, for example.The function MSB s returns the s most significant bits of the argument. Thus, for example, MSB4(111011010) = 1110.6 RMAC Specification6.1 Message FormattingThe first steps of the MAC generation function are to append padding to the message and to partition the resulting string into complete blocks. The padding, denoted PAD, is a single ‘1’ bit followed by the minimum number of ‘0’ bits such that the total number of bits in the padded message is a multiple of the block size. The padded message is then partitioned into a sequence of n complete blocks, denoted M1, M2, …, M n. Thus,M || PAD = M1 || M2 ||…|| M n .If the bit length of M is a multiple of the block size, then PAD = 1 || 0b-1, i.e., a complete block.6.2 Parameter SetsA parameter set is a pair of values for the bit lengths r and m of the two parts of the authentication tag, the salt and the MAC. The parameter sets for RMAC depend on the block size of the underlying block cipher algorithm. A parameter set shall be chosen from Table 1 below; five parameter sets are given for the 128 bit block size, and two for the 64 bit block size. Although parameter set I offers the shortest authentication tags, it is not recommended for general use. The decision to use parameter set I requires a risk-benefit analysis of at least three factors: 1) the relevant attack models, 2) the application environment, and 3) the value and longevity of the data to be protected. In particular, parameter set I shall only be used if the controlling protocol or application environment sufficiently restricts the number of times that verification of an authentication tag can fail under any given pair of RMAC keys. For example,the short duration of a session, or, more generally, the low bandwidth of the communication channel may preclude many repeated trials.Parameter sets II, III, IV, and V are appropriate for general use.Table 1: Parameter Setsb=128 b=64Parameter Set r m r m32I 03264 6464II 0n/a80III 16n/a96IV 64V 128 128 n/aSome of the security considerations that underlie the selection of a parameter set are summarized in Appendix A. The expected work factors for important aspects of the attacks that are discussed in the appendix are summarized for each parameter set in Table 2 in Section A.4.6.3 MAC GenerationThe following is a specification of the RMAC MAC generation function:Input:block cipher CIPH;block cipher keys K1 and K2 of bit length k;parameter set (r, m);message M;salt R of bit length r.Output:message authentication code RMAC(R, M) of bit length m.Steps:toM the padding string PAD, as described in Section 6.1.1. Append2.Partition M || PAD into n blocks M1, M2, …, M n, as described in Section 6.1.3.O1 =CIPH K1(M1).j = 2 to n, do O j= CIPH K1(M j⊕O j-1).4. Forr=0, then K3=K2; else K3 = K2 ⊕ (R || 0k-r).5. If6.Return RMAC(R, M) = MSB m(CIPH K3(O n)).The calculations in Steps 3 and 4 are equivalent to encrypting the padded message using the cipher block chaining (CBC) mode [5] of the block cipher, under the first RMAC key, with the zero block as the initialization vector. However, unlike CBC encryption, in which every output block from Steps 3 and 4 is part of the encryption output (i.e., the ciphertext), in RMAC, the output blocks in Steps 3 and 4 are intermediate results. In Step 6, the block cipher under a newkey is applied to the final output block from Step 4, and the result is truncated as specified in the parameter set. The new key for this final application of the block cipher is obtained in Step 5 by exclusive-ORing the salt into the most significant bits of the second RMAC key.The RMAC MAC generation function is illustrated in Figure 1.6.4 Tag Generation and VerificationThe prerequisites for the authentication process are the establishment of an approved block cipher algorithm, two secret RMAC keys, and a parameter set1 among the parties to the authentication of the data.To generate an authentication tag on a message M, a party shall determine an associated salt R in accordance with Appendix B, generate RMAC(R,M), as specified in Section 6.3, and provide the authentication tag (R, RMAC(R,M)) to the data.To verify an authentication tag (R', MAC'), a party shall apply the RMAC MAC generation function, as specified in Section 6.3, to the received message M' and the received salt R' within the tag. If the computed MAC, i.e., RMAC(R',M'), is identical to the received MAC, i.e., MAC', then verification succeeds; otherwise, verification fails, and the message should not be considered authentic.1 For tag verification, the parameter set is implicit in the bit length of the tag.Appendix A: Security ConsiderationsThe submitters of RMAC present a security analysis of RMAC in [6]. In this appendix, three types of attacks on general MAC algorithms are summarized, and discussed with respect to RMAC: exhaustive key search, general forgery, and extension forgery based on birthday collisions.A.1 Exhaustive Key SearchIn principle, given sufficiently many valid message-tag pairs, an unauthorized party can exhaustively search, off-line, every possible key to the MAC generation algorithm. After recovering the secret key, by this method or any other method, the unauthorized party could generate a forgery, i.e., a valid authentication tag, for any message.The number of RMAC keys is so large that exhaustive key search of RMAC is impractical for the foreseeable future. In particular, for the key size k, which is at least 112 bits for the approved block cipher algorithms, the exhaustive search for the two RMAC keys would be expected to require the generation of 22k-1 MACs. Even if the two RMAC keys are derived from a single master key, as discussed in Appendix B.1, the exhaustive search for the master key would be expected to require the generation of 2k-1 MACs.ForgeryA.2 GeneralThe successful verification of a MAC does not guarantee that the associated message is authentic: there is a small chance that an unauthorized party can guess a valid MAC of an arbitrary (i.e., inauthentic) message. Moreover, if many message forgeries are presented for verification, the probability increases that, eventually, verification will succeed for one of them. This limitation is inherent in any MAC algorithm.The protection that the RMAC algorithm provides against such forgeries is determined by the bit length of MAC, m, which in turn is determined by the choice of a parameter set. The probability of successful verification of an arbitrary MAC with any given salt on any given message is expected to be 2-m; therefore, larger values of m offer greater protection against general forgery.A.3 Extension Forgery Based on a CollisionThe underlying idea of extension forgery attacks is for the unauthorized party to find a collision, i.e., two different messages with the same MAC (before any truncation). If the colliding messages are each concatenated with a common string, then, for many MAC algorithms, including RMAC, the two extended messages have a common MAC. Therefore, the knowledge of the MAC of one extended message facilitates the forgery of the other extended message. The unauthorized party can choose the second part of the forged message, i.e., the common string, but generally cannot control the first part, i.e., either of the original, colliding messages.In principle, collisions may exist, because there are many more possible messages than possible MACs. A collision may be detected by the collection and search of a sufficiently large set of message-MAC pairs. By the so-called “birthday surprise” (see, for example, [7]), the size of this sufficiently large set is expected to be, approximately, the square root of the number of possible MAC strings, before any truncation.For RMAC, the extension forgery requires that the salt values, R, are the same for the two colliding messages, as well as the untruncated MACs, i.e., CIPH K3(O n) in the specification of Section 6.3. Therefore, larger values of the block size, b, and the salt size, r, provide greater protection against extension forgery. In particular, the unauthorized party would have to collect at least 2(b+r)/2 message-tag pairs in order to expect to detect a collision.Moreover, if a parameter set is chosen in which m<b, i.e., if CIPH K3(O n) is truncated to produce the MAC, then the discarded bits may be difficult for an unauthorized party to determine, so collisions may be difficult to detect. Parameter sets in which m<b may also provide some protection against other types of attacks.A.4 Summary of Security Properties of Parameter SetsIn Table 2, the expected work factors for the important aspects of the attacks discussed in Sections A.1-A.3 are summarized for the RMAC parameter sets. The values for exhaustive key search are given for the case in which the two RMAC keys are generated from a single master key as discussed in Section B.1.Table 2: Expected Work Factors for Three Types of Attacks on RMACRMAC Parameter Set Exhaustive Key Search(MAC GenerationOperations)General Forgery(Success Probabilityfor a Single Trial )Extension Forgery(Message-Tag Pairs)I 2k-12-32232 (b=64) or 264 (b=128) II 2k-12-64264III 2k-12-80272IV 2k-12-96296V 2k-12-1282128Appendix B: The Generation of RMAC ParametersB.1 Derivation of RMAC keys from a Master KeyThe two secret RMAC keys, K1 and K2, may be derived from a single master key, K, in order to save bandwidth or storage, at the cost of extra invocations of the block cipher to set up the RMAC keys. For example, let CNST1, CNST2, CNST3, CNST4, CNST5, and CNST6 be constants, i.e., fixed, distinct blocks, and let k and b be the key length and block length of the approved block cipher, as before. If k 4 b, then K1 and K2 may be derived from the set of constants as follows:K1=MSB k(CIPH K(CNST1) || CIPH K(CNST3) || CIPH K(CNST5))K2=MSB k(CIPH K(CNST2) || CIPH K(CNST4) || CIPH K(CNST6)).If k=b, then this definition reduces to K1=CIPH K(CNST1) and K2=CIPH K(CNST2), and thus only two constants are actually required.Similarly, if b<k≤2b, then the definition becomes K1= MSB k(CIPH K(CNST1) || CIPH K(CNST3)) and K2=MSB k(CIPH K(CNST2) || CIPH K(CNST4)), and thus only four constants are required.B.2 Salt GenerationThe salt values associated with messages shall repeat with no more than negligible probability. In particular, the expected probability that the same salt will be associated with two different messages that are authenticated under the scope of any pair of RMAC keys shall be no greater than for random values of salt. Therefore, one approach to meeting the requirement is to generate the salt by an approved deterministic random number generator.Another approach is to ensure that the probability of associating the same salt to different messages is zero, in other words, to generate a nonce to be the salt. For example, the salt may be a counter or a message number.Appendix C: Example Vectors for the MAC Generation FunctionIn this appendix, examples vectors are provided for the RMAC MAC generation function with either the AES algorithm or Triple DES as the underlying block cipher. For each allowed key size of the underlying block cipher, MACs are generated on three messages for each parameter set. The lengths of the three messages, denoted Mlen , are 128 bits, 384 bits, and 400 bits. In addition to the MAC for the given input values, intermediate results are provided. All strings are represented in hexadecimal notation.C.1 RMAC-AES128 Example VectorsC.1.1 RMAC-AES128-I RMAC-AES128, r =0, m =32, Mlen =128 M : 000102030405060708090a0b0c0d0e0f K 1: 000102030405060708090a0b0c0d0e0f K 2: 0f0e0d0c0b0a09080706050403020100 R : n o n e M || PAD : 000102030405060708090a0b0c0d0e0f 80000000000000000000000000000000 O_1: 0a940bb5416ef045f1c39458c653ea5a O_n : 3a3807ffe3cb3e978953017210335f0f K 3: 0f0e0d0c0b0a09080706050403020100 CIPH_K 3(O_n ): bfc3c92e04100777be98f7a93e178381 RMAC (R ,M ): bfc3c92e RMAC-AES128, r =0, m =32, Mlen =384 M : 000102030405060708090a0b0c0d0e0f 101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f K 1: 000102030405060708090a0b0c0d0e0f K 2: 0f0e0d0c0b0a09080706050403020100 R : n o n e M || PAD : 000102030405060708090a0b0c0d0e0f 101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f 80000000000000000000000000000000 O_1: 0a940bb5416ef045f1c39458c653ea5a O_2: 3cf456b4ca488aa383c79c98b34797cb O_3: 7e163e30ea49d32152a51a08a10ec02d O_n : c5b089e3e4710856581f28b42824c651 K 3: 0f0e0d0c0b0a09080706050403020100 CIPH_K 3(O_n ): a3c33ae5f5d19094c5f65faa4ee60696 RMAC (R ,M ): a3c33ae5 RMAC-AES128, r =0, m =32, Mlen =400 M : 000102030405060708090a0b0c0d0e0f101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f 3031 K 1: 000102030405060708090a0b0c0d0e0f K 2: 0f0e0d0c0b0a09080706050403020100 R : n o n e M || PAD : 000102030405060708090a0b0c0d0e0f 101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f 30318000000000000000000000000000 O_1: 0a940bb5416ef045f1c39458c653ea5a O_2: 3cf456b4ca488aa383c79c98b34797cb O_3: 7e163e30ea49d32152a51a08a10ec02d O_n : 6a83b72738a946e319702dfd323fae52 K 3: 0f0e0d0c0b0a09080706050403020100 CIPH_K 3(O_n ): 4577d30eac2b9a438e507ecf22cc5fbd RMAC (R ,M ): 4577d30eC.1.2 RMAC-AES128-IIRMAC-AES128, r =0, m =64, Mlen =128 M : 000102030405060708090a0b0c0d0e0f K 1: 000102030405060708090a0b0c0d0e0f K 2: 0f0e0d0c0b0a09080706050403020100 R : n o n e M || PAD : 000102030405060708090a0b0c0d0e0f 80000000000000000000000000000000 O_1: 0a940bb5416ef045f1c39458c653ea5a O_n : 3a3807ffe3cb3e978953017210335f0f K 3: 0f0e0d0c0b0a09080706050403020100 CIPH_K 3(O_n ): bfc3c92e04100777be98f7a93e178381 RMAC (R ,M ): bfc3c92e04100777 RMAC-AES128, r =0, m =64, Mlen =384 M : 000102030405060708090a0b0c0d0e0f 101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f K 1: 000102030405060708090a0b0c0d0e0f K 2: 0f0e0d0c0b0a09080706050403020100 R : n o n e M || PAD : 000102030405060708090a0b0c0d0e0f 101112131415161718191a1b1c1d1e1f 202122232425262728292a2b2c2d2e2f 80000000000000000000000000000000 O_1: 0a940bb5416ef045f1c39458c653ea5a O_2: 3cf456b4ca488aa383c79c98b34797cb O_3: 7e163e30ea49d32152a51a08a10ec02d O_n : c5b089e3e4710856581f28b42824c651 K 3: 0f0e0d0c0b0a09080706050403020100 CIPH_K 3(O_n ): a3c33ae5f5d19094c5f65faa4ee60696。
EARLY DETECTION OF PROSTRATE CANCER (CAP) BY DETER
专利名称:EARLY DETECTION OF PROSTRATE CANCER (CAP) BY DETERMINING A RATIOINVOLVING PROSTRATE SPECIFIC ANTIGEN(PSA) AND HUMAN GLANDULARKALLIKREIN (HGK-1) CONCENTRATIONS发明人:LILJA, Hans,Lundwall, Ake,Lövgren, Janita申请号:EP96902292.0申请日:19960214公开号:EP0811164A1公开日:19971210专利内容由知识产权出版社提供摘要:The invention relates to a bioaffinity assay of prostate- specific antigen (PSA) comprising the measurement of either the concentration of total PSA (PSA-T), the concentration of free form of PSA (PSA-F) or the concentration of PSA complexed to alpha-1-antichymotrypsin (PSA-ACT), PSA- T being the sum of PSA-F and PSA-ACT. According to the invention, additionally the concentration of human glandular kallikrein (hGK-1) is measured. The concentrations of PSA-T and hGK-1 can be measured in one single assay or in separate assays. The sum of the concentrations of PSA- T and hGK-1 is used to determine the ratio a) PSA- F/(PSA-T+hGK-1) and/or b) PSA-ACT/(PSA-T+hGK-1). Both of these ratios are shown to have clinical utility for the discrimination of prostate cancer and benign prostatic hyperplasia.申请人:LILJA, Hans,Lundwall, Ake,Lövgren, Janita地址:Holländarevägen 28 236 00 Höllviken SE,Mellanvangsvägen 5 223 55 Lund SE,Valtaojantie 34 20810 Turku FI国籍:SE,SE,FI代理机构:Öhman, Ann-Marie, et al 更多信息请下载全文后查看。
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ing. Massimiliana Carelloi M i ili C llMechanical and Aerospace Engineering Department e‐mail: massimiliana.carello@polito.itAutomotive Engineering 2014/2015Part Brake Systems1–systems have the following tasks:vehiclethe vehicle speedto keep a halted vehicle stationary2equipment’s functions can be divided system BBA(or footbrake)system HBA(or auxiliary)system FBA(or handbrake)(h db k)system allows to reduce,directlyduring normal driving or todevice.It supplies,regulates and,if necessary, braking.It terminates at the point where energy source is that part of an energymay be located remotely(compressedforce of the driver.control signal can be conveyed withinhydraulic or electrical means,includingforce.The control force is directly applied:force by the driver by hand or foot;driver or without any action(only pressure in a connection line,or the electricenergy brake systemspassenger cars and two‐wheeled vehicles.Thepedal or hand lever is transmitted to the brakesBowden cable)or hydraulic(master cylinder,wheelassisted or energy assisted brake systemspassengers cars and light commercial vehicles.Itunit)to supplement muscular force with energyhydraulic pressureor non‐muscular energy systemsII variant(a)Separate circuit for the front and rear axlesX variant(b)Diagonal circuit patternHI variant(c)One circuit for the front and a second forfront and rear wheels6Motor vehicle must be equipped with two separate brake system,one of which must be lockable.The brake system must have separate control devices.In the event of a fault in the service brake system,it must still be possible for at least two wheels(not at the same side)to be braked.Brake systems in motor vehicles consist of the following assemblies,which offer in B k t i t hi l i t f th f ll i bli hi h ff i design depending on whether the system is hydraulic or pneumatic:‐energy supply,‐control devices,‐transmission devices,‐control facilities,‐wheel brakes,8Brake‐pressureregulating valves Front direction910brake booster is an enhanced master cylinder setup usedthe amount of pedal pressure needed for braking.employs a booster set up to act with the master cylinder hydraulic pressure to the brakes and/or lower force applied pedalbrake pedal.brake booster usually uses vacuum from the engine intakethe force applied by the pedal to the master cylinder,an extra vacuum pump to enable it.the engine running the brakeineffective on the braking capability.braking process is initiated and controlled through the master cylinder.For the car where it is mandatory two separate brake circuits is used a master cylinder designed as a tandem unit.12force applied to the brake pedal act directly on the push‐rod piston(14),whichmoving on the left.As it does so,it passes over the snifter bore(12)and the fluid pressure chamber(21)can press the float piston(6)to the left as well.valve pin(18)is no longer up against the slotted spring pin(7)as soon as thehas moved about1mm to the left.The valve seal(16)presses against the float (6)to seal and insulate the pressure chamber(3)from the intermediate chamber(8).The pressure in both chamber(3and21)will now increase in response to any additional force at the pedal.At the same time both pistons(6and14)will react to decreasing foot pressure by shifting to the right until the snifter bore(12)is exposed or until the valve pin (18)contacts the slotted spring pin(7)and lifts the valve seal(16)from the float piston(6). This allows the brake fluid to return to the supply reservoir,releasing the pressure in the brake system.131415 between the master cylinder and the rest of the brakecomponent is the key to safe, reliable brake system operationconditions.in dynamic forces from rear to front accompanies thebraking‐process braking.However this rear to front weight shift is not a linear process,but its magnitude increases as a function of deceleration.Thus the need for a supplementary means of reducing the brakepressure at the rear wheels relative to that at the front wheels.This function is assumed by the brake‐pressure regulating valve, that(as a function of manufacturer and vehicle) it willy gusually conform to one of three designs:‐load‐sensitive pressure‐regulating valve,‐pressure‐sensitive pressure‐regulating valve,‐deceleration‐sensitive pressure‐regulatingvalve.16It is required for the vehicles(such as stationwagons)in which high load factors lead topronounced inter‐axle force shifts duringbraking.gThe pressure regulator is attached to thevehicle body,and connected to the rearsuspension via mechanical linkage.The relative displacement from suspensionand body is relayed to a piston located withinthe pressure‐regulating valve’s housing.The piston responds to variations insuspension compression rates by contracting ai i t b t tispring to modify the cycle point.This system adapts the pressure at the rearbrakes to compensate the load changingconditions.1718It is also known like pressure limiters.pIt is employed on vehicles in which thepotential for inter‐axle load shifts isrestricted by limited cargo capacity and alow center of gravity(i.e.sports cars).19It is used in a wide variety ofapplications.The cycle point in these devises,determined by the vehicle’s rate ofdeceleration,that is generally0.3g.The valve incorporates compensationproperties.2021AccumulatorPumpElectrovalve22The EHB is a braking system that combines the functions of a brake servo unit and the ABS equipment including ESP.Mechanical operation of the brake pedal is detected by the actuation unit by means of electronic sensor with redundant backup and a signal is transmitted to the ECU.23。