无偿献血人群人类免疫缺陷病毒抗体和核酸检测分析

无偿献血人群人类免疫缺陷病毒抗体和核酸检测分析
欧阳玲;黄呈辉;陈汝光;黄建国
【期刊名称】《中华实验和临床病毒学杂志》
【年(卷),期】2000(014)003
【摘要】目的了解深圳地区无偿献血人群人类免疫缺陷病毒(HⅣ)感染流行情况。

方法采用酶联免疫吸附试验(ELISA)对无偿献血者进行抗-H1V-1／2筛查，对初查阳性标本用聚合酶链反应(PCR)方法进一步检测外周血单个核细胞(PMBCs)中HIV DNA和血浆中HIV RNA，并对2例无偿献血者PCR扩增阳性片段进行序列测定。

结果在46 095例无偿献血者中，发现抗-HIV1阳性7例，阳性率为
0.015％；在7例抗-HIV-1阳性献血者的PBMCs中均检出HIV DNA阳性，5例血浆中检测出HIV RNA阳性；2例献血者HIV DNA测序分析显示，感染的是
HIV-1 E亚型毒株。

结论深圳地区无偿献血者人群存在HIV E亚型毒株感染，对无偿献血者仍应加强HIV感染的筛查和监测。

%Objective To study the prevalent status of HIV - 1 in population of non - remunerated blooddonors in Shenzhen. Methods 46 095 non- remunerated blood donors were tested for anti- HIV- 1/2 byELISA. The donors of anti- HIV positive were further detected for HIV DNA from peripheral blood mononuclearcells (PMBCs) by nested - PCR and HIV RNA from plasma by RT - PCR. The partial genome of env of 2 blooddonors were sequenced. Results The anti - HIV - 1 was tested positive in 7 of 46 095 voluntary blood donors andthe positive rate was 0.015%. In these 7 non- remunerated blood donors of anti - HIV - 1 positive,7 were posi-tive for
HIV DNA in PMBCs and 5 positive for HIV RNA in plasma. The sequence analysis showed that 2 donorswere infected by HIV - 1 subtype E strains. Conclusion There exists HIV - 1 subtype E infection in population ofnon - remunerated blood donors in Shenzhen. It is essential to detect and monitor strictly the population of non - re-munerated blood donors.
【总页数】4页(P236-238,239)
【作者】欧阳玲;黄呈辉;陈汝光;黄建国
【作者单位】518101深圳市宝安区血站;518101深圳市宝安区血站;518101深圳市宝安区血站;518101深圳市宝安区血站
【正文语种】中文
【中图分类】R1
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